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    The document appears to be a quiz on factors affecting microbial growth and microbial control. It contains 8 multiple choice questions testing knowledge of concepts like phenol coefficient testing of disinfectants, how osmotic pressure and temperature affect microbial growth, and mechanisms of different chemical agents used to control microbes.

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    The document appears to be a quiz on factors affecting microbial growth and microbial control. It contains 8 multiple choice questions testing knowledge of concepts like phenol coefficient testing of disinfectants, how osmotic pressure and temperature affect microbial growth, and mechanisms of different chemical agents used to control microbes.

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    Quiz-Factors-affecting-Mic.-Growth-and-Microbial-Control-S-BIOL317LA-BSY23-Gen.-Microbiology-Lab.-2nd-Sem-SY2022-2023-Quiz-Factors-affecting-Mic.-Growth-and-Microbial-Control-DLSU-D-College_G

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    The document appears to be a quiz on factors affecting microbial growth and microbial control. It contains 8 multiple choice questions testing knowledge of concepts like phenol coefficient testing of disinfectants, how osmotic pressure and temperature affect microbial growth, and mechanisms of different chemical agents used to control microbes.

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    S-BIOL317LA BSY23 Gen. Microbiology Lab. 2nd Sem SY2022-2023


    Quiz - Factors affecting Mic. Growth and Microbial Control

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    Finished

    Question 1
    Using Staphylococcus aureus as the test organism, the following results in a phenol coefficient test have been
    obtained.

    Test disinfectant (5 Test disinfectant (10


    Phenol (5 mins) Phenol (10 mins)
    mins) mins)
    1:80 - - - -
    1:90 - - - -
    1:100 + - - -
    1:125 + + + -
    1:150 + + + +

    PC = A / B
    Identify the value of A. _____

    Identify the value of B. _____

    What is the phenol coefficient of the test disinfectant? _____

    Is the test disinfectant effective? _____

    Response: 1:125

    Response: 1:100

    Response: 1.25

    Response: yes

    Question 2
    _____ affects the amount of water availability and further affects the survival of the microorganism.

    Response: Osmotic Pressure

    Question 3
    Bacteria pH 3.5 pH 7.0 pH 9.0 4 oC 10 oC 30 oC 40 oC 60 oC
    _____ + + -
    _____ - + -
    _____ + + + + -
    _____ + + - - -

    Identify the group of bacteria that exemplifies the results in the table above (+ growth, - no growth).

    Response: Acidophile

    Response: Neutrophile

    Response: Mesophile

    Response: Psychrophile

    Question 4
    Match the mechanism of action with the chemical agent used to control microbial growth .

    Response: Surfactants in soaps => Breakdown oily substances and mechanically remove microbes through
    scrubbing.

    Response: Disinfectant such as Lysol => Act by denaturing the proteins and disrupting cell membranes.

    Response: Halogens such as Iodine => Inhibits protein function and is a strong oxidizing agent.
    Response: 70 % Ethyl Alcohol => Act by denaturing the proteins and disrupting cell membranes.

    Response: Silver nitrate => Denaturation of enzymes and other essential protein.

    Response: Chlorhexidine => Disruption of plasma membrane.

    Question 5

    Bacteria 0% salt 7.5% salt 15% salt 30% salt

    _____ - + + +

    _____ + + - -

    Identify the group of bacteria that exemplifies the results in the table above (+ growth, - no growth)

    Response: Halophile

    Response: Halotolerant

    Question 6
    This group have membrane lipids that are more saturated and have higher melting points – remain intact at higher
    temps.
    _____

    Response: Thermophiles

    Question 7
    The enzymes, transport systems and protein synthetic mechanisms of this group function well at low temp.
    _____

    Response: Psychrophiles

    Question 8
    In testing the effectivity of a disinfectant, to which substance do we compare the test chemical?
    _____

    Response: Phenol

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