Republic of the Philippines

Department of Education
Region XI- Davao Region
Schools Division of Panabo City
PANABO CITY NATIONAL HIGH SCHOOL

PROJECT TITLE: The Effectiveness of Pomelo (Citrus maxima) Peel,

Lanzones (Lancium domesticum) Peel Extracts as Repellents Against
Aedes egypti
NAMES OF PROJECT PROPONENT/S: Avila, Axl Rotsen T. , Malubay,
Prince Joshua O., and Zafra, Keisha DeAnne U.
PROJECT DURATION: 1 month GRADE LEVEL: 9
EMAIL: [email protected],
[email protected]
[email protected]
CONTACT NUMBER: 09121280627

CATEGORY OF RESEARCH THEME

_____ Physical Science _____ Food Safety
__/_ _ Life Science _____ Water Conservation
__ _ Robotics and Intelligent Machines _____ Renewable Energy
_____ Mathematics and _____ Cyber Security
Computational Science ____ Traffic/Road Congestion
_/___ Health
_____ Individual _____ Disaster Mitigation
__/__ Team ____ Agriculture and Environment
____Others, specify: __________

EXECUTIVE SUMMARY
The global occurrence of dengue fever has significantly risen in recent decades.
A large proportion of cases show no symptoms or only mild symptoms and are
managed by the individuals themselves. Consequently, the actual number of dengue
cases remains unreported. Additionally, numerous cases are misdiagnosed as other
fever-related illnesses. Based on modeling estimates, it is believed that there are
approximately 390 million dengue virus infections each year, with a 95% confidence
interval ranging from 284 million to 528 million. Among these infections, around 96
million cases (ranging from 67 million to 136 million) exhibit clinical manifestations,
irrespective of their severity. Another study examining dengue prevalence indicated
that approximately 3.9 billion individuals are at risk of contracting the dengue virus.
Despite the risk being present in 129 countries, approximately 70% of the actual
burden is concentrated in Asia.

I. INTRODUCTION

Mosquitoes remain a global concern, with their presence spanning across nearly every
continent except Antarctica. The seriousness of the mosquito problem is multifaceted.
These insects are notorious for transmitting deadly diseases such as malaria, dengue
fever, Zika virus, West Nile virus, and various encephalitides, posing significant
public health challenges in affected regions. Beyond disease transmission, mosquito
bites can cause allergic reactions, leading to itching and skin irritation. Furthermore,
some mosquito species target livestock and poultry, causing economic losses in
agriculture and livestock industries. In regions where mosquito-borne diseases are
prevalent, tourism can also be impacted as travelers may be deterred by the risk of
contracting these diseases. The severity of the mosquito problem varies by location
and environmental factors, with tropical and subtropical regions facing year-round
challenges, while temperate areas experience seasonal mosquito nuisances during
warmer months. Addressing these issues requires ongoing efforts in mosquito control,
including the use of insecticides, mosquito nets, repellents, and public health
campaigns, along with research into innovative control strategies. For the latest
information on the current status of mosquito-related problems in specific regions,
consulting local health authorities and recent news sources is recommended.

Repellent such us OFF lotion is great example of initiatives undertaken to solve the
problem because it is easy to apply and can protect you anywhere.

There are disadvantages/weakness of OFF lotion. Although light and fast absorbing is
intense to the skin and Off Lotion is made up of poison which is its main ingredient to
repel mosquitos. The very reason why I felt it harsh on parts
of my body which is sensitive, and it sometimes gives you that humid feeling in your
skin. It is also the reason why there was discoloration noticed in the sachet.
The researchers' solution presents a distinctive approach. First, one fruit peel is
subjected to a drying process, while the other requires a specialized method for
collecting its extract. Subsequently, the researchers conduct tests on mosquitoes in a
transparent enclosure, systematically assessing the efficacy of the fruit peel extract as
a mosquito repellent. This method involves observing how the extracted substance
interacts with the mosquitoes and whether it effectively deters them from the enclosed
area.

This study aims to find an effective mosquito repellent with the use of lanzones peels
and pomelo peels. The fruit peels will be tested one by one and also together, hence, it
can determine how effective it is compared to each other.

The significance of this study is to make individuals' evironment mosquito-free. This

aid the Republic Act No. 10043,an act establishing Malaria, tropical and infectious
diseases research and treatment.

The incidence of dengue fever has increased sharply worldwide over the past few
decades. This means that the increase in dengue cases has been observed not only in
specific regions but across the globe. The majority of cases are either asymptomatic or
mild and self-managed, and therefore the true number of dengue cases is not reported
globally. Many cases are also misdiagnosed as other febrile diseases in various parts
of the world. Modeling estimates indicate that there are approximately 390 million
dengue virus infections per year on a global scale, with a 95% confidence interval
ranging from 284 million to 528 million. Out of these cases, around 96 million (with a
range of 67-136 million) are clinically manifest, regardless of severity. Another study
on the prevalence of dengue estimated that a staggering 3.9 billion people around the
world were at risk of being infected with the dengue virus. Despite the risk of
infection existing in 129 countries, 70% of the actual disease burden is concentrated
in the Asian region.1

1
World Health Organization: WHO and World Health Organization: WHO. “Dengue and
Severe Dengue.” Www.Who.Int, March 17, 2023. https://www.who.int/news-room/fact-
sheets/detail/dengue-and-severe-dengue.
 In the city of Mati in the eastern province of Davao in the southern Philippines,
city health officials issued a dengue fever advisory due to an increase in mosquito-
borne viral disease. In January 2022, according to Dr. Ben Hur Catbagan Jr., Mati
City Health Officer, 29 cases of dengue fever were reported, of which 5 were positive
for COVID-19. Nine of the 26 barangays (villages) are affected by dengue: Brgy.
Center, Brgy. Side by side, Brgy. Ghé thăm, Brgy. Don Enrique Lopez, Brgy. Don
Martin Marundan, Brgy. Don Salvador Lopez, Brgy. Macambol, Brgy. Matiao and
Brgy. Sainz.2

Barangay New Visayas had the highest number of dengue cases with eight,
followed by San Vicente with six cases, while New Pandan, Gredu, Cagangohan, Sto.
Niño all have three cases each. Two cases were recorded in Barangays San Francisco
and San Pedro, and one case each for Barangays Dapco, Katipunan, JP Laurel,
Tibungol, Manay, and Quezon. “Gitutokan sa Panabo City Health Office ang kaso sa
Dengue sa siyudad, karong tuiga posibleng mag-taas kini tungod sa three-year dengue
cycle (The Panabo City Health Office is monitoring the cases of Dengue in the city
which may increase due to the three-year dengue cycle),” the Panabo LGU said in a
statement published online on Monday, February 21. In 2019, the city recorded 933
cases of dengue, which dropped to 299 cases in 2020 and further declined to 285
cases in 2021. The Panabo LGU said the CHO continues to provide intervention
activities against dengue such as misting, indoor residual spraying, vertor
surveillance, and the 4-S campaign of the Department of Health which stands for
search and destroy mosquito-breeding sites, secure self-protection measures, seek
early consultation, and support fogging and spraying in hotspot areas.3
Aedes aegypti was formerly established in southern Europe from the late 18th to
mid-20th centuries. It is know for spreading victor borne diseases like yellow fever,
chikungunya, zike fever and more. Us researchers noticed that most of the fruit peels
are thrown away in the trash so we found a way to utilize this problem. The
researchers also noticed that the victor borne diseases victim rates has gone up so we
2
Press Release. “Philippines: Dengue Advisory in Mati City - Outbreak News Today.”
Outbreak News Today, February 12, 2022. https://outbreaknewstoday.com/philippines-
dengue-advisory-in-mati-city-78511/.

3
Mendoza, Iona Finlay C. “Panabo Records 36 Dengue Cases since January.” SUNSTAR,
February 22, 2022. https://www.sunstar.com.ph/article/1921725/davao/local-news/panabo-
records-36-dengue-cases-since-january.
tried to find a way to solve both these problem together. The researchers picked
lanzones (Lancium domesticum) peels, and pomelo (Citrus maxima) peels because
these fruits waste a lot of peel after eaten.

The goal of mosquito repellent operations is to prevent any adult dengue

mosquitoes that may be carrying the dengue virus from biting people. Mosquitoes
become infected with the virus after biting and sucking the blood of a person with
dengue fever. This study will benefit the public, parents, and children by providing
more information regarding the effects of Pomelo (Citrus Maxima), and Lanzones
(Lancium Domesticum) peels extract as mosquito repellent. The results of this study
will also help other researchers to do more research about natural ways to prevent
mosquitoes.

II. RATIONALE/SIGNIFICANCE

RATIONALE

The viral infection transmitted by aedes aegypti, mosquito-borne disease,

affect almost 700 million people each year and about more than a million people
resulted to dying. It is rapidly expanding disease in the tropics of the world, and it has
become the most important arbovirus disease to people over 20 years period. There
are over 2 billion people at risk of infection, and millions of cases occur each year.
Authorities reported roughly 226,497 cases of dengue fever in the Philippines in 2022,
roughly 78,208 cases in 2021, and roughly 90,135 cases in 2020. And so our study
aims to reduce the risk of people contracting this terrible disease with the use of fruit
peels extract as a repellent for Aedes aegypti. The finding of this study will help assist
society and people’s welfare as we concentrate on producing repellents from fruit
peels. In addition, it will help poor families or families from remote areas to fight off
the risk of Aedes aegypti.4

SIGNIFICANCE
The findings of this study provide empirical evidence in favor of the Mosquito
Control Act 1980 (No. 63 of 1980), a legislative framework aimed at strengthening
4
Crisis. “Philippines: Elevated Dengue Fever Activity Reported Nationwide through
February.” Philippines: Elevated Dengue Fever Activity Reported Nationwide Through
February | Crisis24, March 6, 2023. https://crisis24.garda.com/alerts/2023/03/philippines-
elevated-dengue-fever-activity-reported-nationwide-through-february.
the enforcement of mosquito control measures within the jurisdiction of Niue. The
primary objective of this act is to safeguard public health by mitigating the risks posed
by mosquito-borne diseases. To achieve this, the legislation imposes a range of
specific obligations on property owners and premises occupiers, mandating them to
undertake defined measures that effectively prevent mosquito breeding and the
proliferation of these disease vectors.

III. SCIENTIFIC BASES/THEORETICAL FRAMEWORK

 Lanzones Peels Effective Natural

d Pomelo Peels t
Test and Assessment Plant Based
repellent.

Figure 1. Theoretical Framework

POMELO (Citrus Maxima) PEEL

Pomelo (Citrus maxima) shows a great mosquito repellent activity due to its
major active constituent, Limonene. In this study, the researchers formulate mosquito
repellent spray from pomelo (Citrus maxima) peel extract in two concentrations
(100% and 75%).
Pesticide products containing limonene are used for flea and tick control on pets, as
an insecticide spray, an outdoor dog and cat repellent, a fly repellent tablecloth, a
mosquito larvicide, and an insect repellent for use on humans.5

A study about Pomelo which utilized the experimental and quantitative research
design which involved the preparation of five identical mosquito traps with the
dimensions of 18x 15x 8 - inches covered with iron sheets for trapping of the
mosquitoes. One for the control (distilled water), one for 100% treatment of pomelo
peeling extract, another for 75% treatment of pomelo peeling extract and lastly, one
for the commercial repellant. These were placed in a Styrofoam container where it
was put in each of the mosquito traps containing inside the attractant was composed
of two (2) tablespoons of sugar and 1 ½ tablespoon of yeast with one hundred fifty
(150) Ml of distilled hot water. The treatments were put at 6o'clock in the afternoon
until the next day after twenty-four (24) hours of observation. Analysis of Variance
(ANOVA), and Duncan Multiple Range Test (DMRT) were used for the statistical
treatment. The research findings showed the effects of the different treatments in
repelling mosquitoes are highly significant. Furthermore, the 100% pomelo peeling
extract concentration and commercial mosquito repellant are not significantly
different from each other. It concluded that the different treatments of the pomelo

5
Poral, Mylyn L. “The Efficacy of Mosquito Repellent Spray from Pomelo Peel Extract on
New Zealand Albino Rabbits (Oryctolagus Cuniculus).” BAHÁNDÌAN, Institutional
Repository of Central Philippine University, 2020.
https://repository.cpu.edu.ph/handle/20.500.12852/1850.
peeling extract concentration can repel mosquitoes. Moreover, the 100% pomelo
peeling extract concentration is as effective as the commercial mosquito repellant in
repelling mosquitoes. Further studies to use other extraction techniques such as
percolation and decoction are recommend. Also, to maximize the other plant such as
pomelo juice, leaves, roots and bark can be used. There should be succeeding studies
to compare the repellence of anopheles gambiae and aedes aegypti using pomelo
peeling extracts. Moreover, other plants similar to pomelo which is abundant to
locality can be studied as mosquito repellant.6

LANZONES (Lancium Domesticum) PEEL

Lanzones fruit were taxonomically and thoroughly washed before it was

extracted on the day of the experiment. Results showed that the lanzones peelings'
extract had a mean protection percentage of 93.33% while the positive control had a
mean protection percentage of 96.19%. This means that the lanzones peelings' extract
showed a 2.86% difference from that of the positive control containing 25%
Diethyltoulamide (DEET) which indicates a very promising result as it showed no
significant difference from the commercial insect repellent. Since the average
protection rate of the experimental set-up (93.33%) is much closer to the value of the
positive control (96.19%) than of the negative control (86.67%), researchers conclude
that the extract is positively effective as a repellent. Also, the positive control used in
the experiment, which is a commercial mosquito repellent containing 25% DEET,
showed a high percentage protection and is therefore an effective mosquito repellent.
Likewise, the negative control also showed a repellent characteristic, though not that
high compared to the extract.7

IV. OBJECTIVES OF THE STUDY

6
Almas, Jochel. “Pomelo (Citrus Maxima) as Natural Mosquito Repellant.,” n.d.
https://www.herdin.ph/index.php?view=research&cid=68907.

7
Acapulco, Don Francis. “The Effectivity of Lanzones (Lansium Dometicum) Peelings’
Extract as Mosquito Repellant.,” n.d. https://www.herdin.ph/index.php?
view=research&cid=60694.
General Objective:

The objective of this study is to produce an effective and cheap fruit base
mosquito repellent so that those who live in rainy places such as the Philippines and
other countries that share the same climate.

Specific Objectives:

1. To determine the effects of different concentration of Pomelo (Citrus maxima)

Peel Extract as repellents against Aedes egypti.
2. To determine the effects of different concentration of Lanzones (Lancium
domesticum) Peel extracts as repellents against Aedes egypti.
To compare which among the three extracts is efficient in repelling Aedes

V. REVIEW OF RELATED LITERATURE

Aedes aegypti

Aedes aegypti, the yellow fever mosquito, is a mosquito that can spread dengue
fever, chikungunya, Zika fever, Mayaro and yellow fever viruses, and other disease
agents. The mosquito can be recognized by black and white markings on its legs and a
marking in the form of a lyre on the upper surface of its thorax. This mosquito
originated in Africa, but is now found in tropical, subtropical and temperate regions
throughout the world.The Aedes aegypti mosquito can transmit the viruses that cause
dengue fever. The female mosquito lays eggs in containers with water and plants near
the home. It bites people and animals. This species can survive year-round in tropical
and subtropical climates.

Pomelo (Citrus maxima) Peel

Pomelo peel as a by-product from pomelo consumption is rich in various

nutrients and functional compounds, while most of the by-product is disposed as
wastes. The utilization of pomelo peels could not only result in valued-added
products/ingredients, but also reduce the environmental threats. By mainly reviewing
the recent articles, pomelo peels could be directly used to produce candied pomelo
peel, tea, jams, etc. Additionally, functional components (essential oils, pectin,
polyphenols, etc.) could be extracted from pomelo peels and applied in food,
pharmaceutical and chemical fields. The extraction methods exerted important
influences on the composition, physicochemical properties, bioactivities and
structures of the resultant fractions. Furthermore, pomelo peel was exploited to make
adsorbents, bioethanol, etc. For the future investigations, the functionality- or
bioactivity-oriented regimes to recovery valuable components from pomelo peel
should be developed in an economic, effective and eco-friendly way and their
applicability in large-scale production should be addressed.8
8
Xiao, Li, Fayin Ye, Yun Zhou, and Guohua Zhao. “Utilization of Pomelo Peels to
Manufacture Value-Added Products: A Review.” Food Chemistry 351 (February 15, 2021):
129247. https://doi.org/10.1016/j.foodchem.2021.129247.
 Related research about pomelo (Citrus maxima): Water distillation was used
in extracting Pomelo (Citrus maxima) peel oil. 100% pomelo (Citrus maxima) peel
extract mosquito repellent spray contains pure essential oil. Coconut oil was used to
dilute 75% pomelo (Citrus maxima) peel extract mosquito repellent spray
formulation. Mosquito larvae for the repellency testing were collected from stagnant
waters and were put in different containers provided with a small screen bond on top,
where it was left to develop into pupae and become adult mosquitoes. The test
subjects used in the repellency testing were rabbits, where the rabbit’s skin was
shaved and divided into four (4) quadrants. The formulated concentration, together
with the positive control (no touch mosquito repellent spray) and negative control
(mineral oil) were applied on different quadrants on the rabbit’s shaved skin. The
rabbits were then exposed to mosquitoes for fourteen (14) hours and were checked
every 2 hours for mosquito bites. The gathered data and results showed that there
were only one to none mosquito landings and mosquito bites on the tested area. The
data analyzed and compared using ANOVA showed a significant difference existed in
the repellency activities of 100% formulation and 75% formulation of pomelo peel
spray to Positive control (No touch mosquito repellent spray) and Negative control
(Mineral oil).9

Lanzones (Lancium domesticum) peel

Lanzone fruit is a sweet, delicious, round to oval berry native to Malayan
Peninsular tropical fruiting trees in the Mahogany family. Its refreshingly sweet and
tangy flavor is appreciated by many fruit lovers in the Southeast Asian countries.
Lanzones are known for its sweet and sour taste, but it becomes bitter if you taste its
seed. They are available from August to December.
Lanzones peels can be used as natural mosquito repellents. Drying and burning
the rind produces a smell that repels mosquitoes (Nutrition and you, 2022).

Related research about lanzones (Lancium domesticum) peel

Naturally breed adult mosquitoes were tested in a mosquito cage by the time
they reached 4-5 days old and were lured using extract from lanzones fruit peeling at
the experimental group, lactic acid as our positive control, and distilled water as the
negative group. Lanzones fruit were taxonomically and thoroughly washed before it
was extracted on the day of the experiment. Three (3) trials were made for each set-up
and results of the lanzones peelings' extract were compared to the control of this
experiment. Results showed that the lanzones peelings' extract had a mean protection
percentage of 93.33% while the positive control had a mean protection percentage of
96.19%. This means that the lanzones peelings' extract showed a 2.86% difference
from that of the positive control containing 25% Diethyltoulamide (DEET) which
indicates a very promising result as it showed no significant difference from the
commercial insect repellent. Since the average protection rate of the experimental set-
up (93.33%) is much closer to the value of the positive control (96.19%) than of the
negative control (86.67%), researchers conclude that the extract is positively effective

9
Poral, Mylyn L. “The Efficacy of Mosquito Repellent Spray from Pomelo Peel Extract on
New Zealand Albino Rabbits (Oryctolagus Cuniculus).” BAHÁNDÌAN, Institutional
Repository of Central Philippine University, 2020.
https://repository.cpu.edu.ph/handle/20.500.12852/1850.
as a repellent. Also, the positive control used in the experiment, which is a
commercial mosquito repellent containing 25% DEET, showed a high percentage
protection and is therefore an effective mosquito repellent. Likewise, the negative
control also showed a repellent characteristic, though not that high compared to the In
the interest of forthcoming studies, the researchers would like to endorse the
following ideas: Use other species of mosquito aside from Aedes aegypti as testing
subject. Use other positive and negative control in performing the experiment. More
information in attracting different types of mosquitoes. Isolation of volatile
components of the extract that may contribute to greater efficacy in repelling
mosquitoes. Qualitatively identify chemicals found in the extract that may harm
humans and animals. Use readily, standardized equipment or reagent in performing
the.10

Mosquito-Borne Diseases
Mosquito-borne diseases and illnesses are caused by bacteria, viruses, or
parasites transmitted by mosquitoes. They can transmit diseases without being
affected themselves and are responsible for over 1 million deaths each year. Nearly
700 million people get a mosquito-borne illness each year. The most prominent
mosquito-borne diseases include malaria, West Nile virus, yellow fever, dengue,
chikungunya, and Zika virus. In the United States, the instances of mosquito-borne
diseases have skyrocketed in the past years:

VI. METHODOLOGY

This study will undergo 7 phases namely; Phase I: Collection of Materials,

Phase II: Preparation of fruit peels extract, Phase III: Extraction of fruit peels, Phase
IV: Collection of mosquitoes, Phase V: Phytochemical screening, Phase VI:
Treatments, Phase VII: Risk, safety, and disposal.

10
Don Francis P. Acapulco, Jay Rendel A. Cesa, Chona G. Mandajoyan, Ray Benedict M. Pasaol,
Sharlaine Ruth Seguerra, Vitaliano V. Fernandez.” The effectivity of Lanzones (Lansium dometicum)
peelings' extract as mosquito repellant.” HERDIN, 2015. https://www.herdin.ph/index.php?
view=research&cid=60694.
 Phase III: Phase II:
Phase I: Collection
Extraction of fruit Preparation of
of material
peels fruits Peels Extract

Phase IV: Phase V:

Phase VI:
Collection of Phytochemical
Treatments
mosquitoes screening

Phase VII: Spray Phase VIII: Risk,

test safety, and disposal

Figure 1. The 7 Phases

PHASE 1: COLLECTION OF MATERIALS

The researchers will buy fruits from the Fruit Stand and as for the cage that the
researchers will use is that they will buy from a hardware store.
Figure 2, Pictures of Durian Rind (left ), Pomelo Peel (centre), Lanzones Peel (right)

PHASE 2: PREPERATIONS OF FRUIT PEELS EXTRACT

Pomelo Peel
With a fresh pomelo, cut off the end of the pomelo where the stem emerges,
slicing about 0.5 inches into the rind. Then, cut the rind into 7-8 vertical sections with
the use of bare hands.

Lanzones Peel
Preparing the a fresh and clean Lanzones, peel it with the use of knife. Make
sure the lanzones peelings were sun dried for a few days. When it is fully dried,
pulverized using a mortar and pestle. After that, mix ana equal amount of starch and
pulverized lanzones peelings. Lastly, add water to make a paste-like consistency.

For this preparation, the researchers will need the following materials: Mortar, pestle,
2L of water,1kg Lanzones (Lancium domesticum) peel, and Pomelo (Citrus maxima)
peel from 2 pomelo fruit.

PHASE 3: EXTRACTION OF FRUIT PEELS

Extracting pomelo (Citrus maxima) peel

Using the squeezing method, the fresh pomelo peel is placed into a screw
squeezer after being soaked with lime water, meanwhile an appropriate amount of
spray water is used to elute the essential oil from the oil cell tissue, and then the
essential oil is filtered with screen cloth, settles, and is separated by a high-speed
centrifuge so as obtain a crude product of the essential oil, which then undergoes
solvent extraction and vacuum fractionation to get terpenoids eliminated.

Extracting lanzones (Lancium Domesticum) peel

The process for extracting Lanzones (Lancium domesticum) is different from the
other two because instead of squeezing it, the researchers will dry it. The researchers
will do it by putting the Lanzones peels that has been cut under the sun for 24 - 48
hours. After that, the researchers will pulverize the dried peels into powder.

PHASE 4: COLLECTION OF MOSQUITOES

The easiest way to catch Mosquitoes is to use a net designed for baby brine
shrimp. Despite living underwater, mosquito larvae breathe air. And to get it, they
need to come up to the surface. It is likely that the mosquito larvae will wriggle away
when you stand over the top of your container. After the researcher obtain a specific
amount of mosquito larvae, the researchers then have to take care of it for 7 days or
until they grow into adult mosquitoes.

PHASE 5: PHYTOCHEMICAL SCREENING

Phytochemical screening is the analysis of plant extracts and it shows the

existence of various beneficial substances such as alkaloids, tannins, saponins,
flavonoids, steroids, terpenoids, resins, cardiac glycosides, phenolic compounds, and
coumarins. All the methanol/DCM extracts from the plants examined displayed
encouraging antimycobacterial effects. Most of the selected plant extracts
demonstrated minimal levels of acute toxicity, except for A. coriaria, suggesting they
could be safely used in the development of herbal products.11

Phase 7: Test
The test will be conducted in Panabo City National High School, LRC building,
Chemistry lab. Before test, a 1 liter of water, lanzones peel extract, Pomelo peel
extract and mosquitoes should be prepared.
Caging the mosquitoes in a box and the researcher will

Figure 4. Test

11
Oloya, Benson, Jane Namukobe, Willy Ssengooba, Mathias Afayoa, and Robert Byamukama.
“Phytochemical Screening, Antimycobacterial Activity and Acute Toxicity of Crude Extracts
of Selected Medicinal Plant Species Used Locally in the Treatment of Tuberculosis in
Uganda.” Tropical Medicine and Health 50, no. 1 (February 17, 2022).
https://doi.org/10.1186/s41182-022-00406-7.
PHASE 6: TREATMENTS
To – commercial like deet idk
T1 – 1 ml + 10 ml water
T2 – 2 ml + 10 ml water
T3 – 3 ml + 10 ml water
T4 – 4 ml + 10 ml water
T5 – 5 ml

Figure 3. Treatments

Pomelo (Citrus maxima) peel Lanzones (Lancium domesticum)

peel
T0 100ml water 100ml water
T1 1ml pomelo peel extract + 10ml 1ml lanzones peel extract + 10ml
water water
T2 2ml pomelo peel extract + 10ml 2ml lanzones peel extract + 10ml
water water
T3 3ml pomelo peel extract + 10ml 3ml lanzones peel extract + 10ml
water water
T4 4ml pomelo peel extract + 10ml 4ml lanzones peel extract + 10ml
water water
T5 5ml pomelo peel extract 5ml lanzones peel extract

Phase 8: safety, risks, and disposal

 DISPOSAL

Organic solvents should be collected in a container with a tight-fitting lid

and labeled as hazardous waste. Corrosive liquids, such as strong acids and bases,
should be disposed of as hazardous waste. However, if appropriately diluted to a
pH between 5.5 and 10.5, these can go down the drain.

RISKS

The risk of this experiment is not a lot when performing, because using
hazardous chemicals are not required in this experiment. But one of the risk
when performing this experiment when the mosquitoes escaped from their cage
where they will be kept.

SAFETY

Safety is one of the most important rules while conducting an experiment.

First of all, wear safety clothing to avoid spilling chemicals into your body, such
as lab coat, goggles, and safety gloves. Secondly, avoid eating and drinking while
conducting the experiment, in a lab full chemical, it is not safe to eat and drink.
Do not taste or sniff chemicals inside the lab. Behave when conducting an
experiment to avoid breaking fragile materials and ruining the experiment. And
lastly, dispose waste properly to avoid harm.

VII. EXPECTED OUTPUTS AND POTENTIAL IMPACTS

The researchers expect this study to be successful. It will help a lot of

people and or economy because dengue. According to official’s data released,
372 cases of the mosquito-borne viral infection were reported from January 1 to
July 30, 2022. This compared to 65 cases reported during the same period in
2021.

VIII. WORKPLAN AND TARGET DELIVERABLES

DURATION START & MATERIALS
NEEDED
(Days or TASK TARGET END
Weeks) DATES (include prices)

Collecting the Cage for the arm-in-

Collecting peels and the cage test,
2 week
materials cage for cage mosquitoes, peel
test extracts.
3 week Collecting Our target is Brine shrimp net
mosquito larvae to get as many
 mosquito
larvae as
possible using
a brine
shrimp net
To identify
which among
Extracts of Pomelo
the Pomelo
peel (Citrus
peel (Citrus
Maxima) and
Maxima), and
Lanzones peel
Lanzones peel
1 day Testing (Lansium
(Lansium
Paraticum), Cage
paraticum)
design for mosquito
extracts is
tests and adult
effective as
mosquitoes
mosquito
repellent.
COST
3k Pesos
ESTIMATES
IX. REFERENCES:

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