1) The document provides measurements of 5 cells at different magnifications, including width, distance measured, and time measured over 5 trials to calculate the rate of streaming.
2) It notes that 200 μM of cytochalasin C dissolved in DMSO was added in a volume of 40 microL to examine the effect on rate of streaming after 10 minutes.
3) The rate of streaming was measured again 10 minutes after adding cytochalasin, showing a decrease, and recovery would be measured at 5, 10, and 20 minutes.
1) The document provides measurements of 5 cells at different magnifications, including width, distance measured, and time measured over 5 trials to calculate the rate of streaming.
2) It notes that 200 μM of cytochalasin C dissolved in DMSO was added in a volume of 40 microL to examine the effect on rate of streaming after 10 minutes.
3) The rate of streaming was measured again 10 minutes after adding cytochalasin, showing a decrease, and recovery would be measured at 5, 10, and 20 minutes.
1) The document provides measurements of 5 cells at different magnifications, including width, distance measured, and time measured over 5 trials to calculate the rate of streaming.
2) It notes that 200 μM of cytochalasin C dissolved in DMSO was added in a volume of 40 microL to examine the effect on rate of streaming after 10 minutes.
3) The rate of streaming was measured again 10 minutes after adding cytochalasin, showing a decrease, and recovery would be measured at 5, 10, and 20 minutes.
1) The document provides measurements of 5 cells at different magnifications, including width, distance measured, and time measured over 5 trials to calculate the rate of streaming.
2) It notes that 200 μM of cytochalasin C dissolved in DMSO was added in a volume of 40 microL to examine the effect on rate of streaming after 10 minutes.
3) The rate of streaming was measured again 10 minutes after adding cytochalasin, showing a decrease, and recovery would be measured at 5, 10, and 20 minutes.
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Take a photo of your prepared sample slide, indicating the location of your five cells:
At 1x magnification (without the microscope)
At 40x magnification At 100x magnification right most middle top/right size masurements Cell 1 SW 2 SE 3 NE 4 NW 5 S of NW width (epd) 9 15 7 17 8 width (um) 90.909 151.515 70.707 171.717 80.808 r.o.s before adding cytochalasin distance measured 202.02 303.03 303.03 808.08 303.03 time measured rep 1 6 14 11 18 9 time measured rep 2 6 20 11 18 7 time measured rep 3 5 15 10 29 7 time measured rep 4 6 15 11 23 10 time measured rep 5 6 14 10 18 10 rate of streaming rep 1 33.67 21.645 27.54818 44.89333 33.67 rate of streaming rep 2 33.67 15.1515 27.54818 44.89333 43.29 rate of streaming rep 3 40.404 20.202 30.303 27.86483 43.29 rate of streaming rep 4 33.67 20.202 27.54818 35.13391 30.303 rate of streaming rep 5 33.67 21.645 30.303 44.89333 30.303 adding cytochalsin Which cytochalasin did you add? C What was its concentration? 200 μM What was it dissolved in? DMSO (Dimethyl sulphoxide) How much did you add? 40 microL r.o.s 10min after adding cytochalasin distance measured 232.323 353.535 191.919 595.959 353.535 time measured 5 10 10 20 10 rate of streaming 46.4646 35.3535 19.1919 29.79795 35.3535 recovery at 5 min distance measured time measured rate of streaming recovery at 10 min distance measured time measured rate of streaming recovery at 20 min distance measured time measured rate of streaming
Thesis Presentation - ISOLATION AND TESTING FOR THE DISEASE-REDUCING EFFECTS AGAINST RICE BACTERIAL LEAF BLIGHT OF SOIL BACTERIA FROM CHỢ GẠO, TIỀN GIANG