Scribd Logo
Upload

Welcome to Scribd!

  • 166 views

    Lead Acetate Test GRP 7

    Uploaded by

    jeannikkav
    The lead acetate test is used to detect the presence of sulfur-containing amino acids like cysteine and cystine in a sample. When these amino acids are heated with a strong alkali and lead acetate under alkaline conditions, it forms a black precipitate of lead sulfide. A positive result is indicated by the black precipitate, showing the presence of cysteine or cystine, while a negative result has no precipitate, showing their absence. The test helps distinguish sulfur from non-sulfur containing amino acids.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Lead Acetate Test GRP 7

    Uploaded by

    jeannikkav
    166 views8 pages
    The lead acetate test is used to detect the presence of sulfur-containing amino acids like cysteine and cystine in a sample. When these amino acids are heated with a strong alkali and lead acetate under alkaline conditions, it forms a black precipitate of lead sulfide. A positive result is indicated by the black precipitate, showing the presence of cysteine or cystine, while a negative result has no precipitate, showing their absence. The test helps distinguish sulfur from non-sulfur containing amino acids.

    Original Description:

    Laboratory notes

    Original Title

    LEAD-ACETATE-TEST-GRP-7

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The lead acetate test is used to detect the presence of sulfur-containing amino acids like cysteine and cystine in a sample. When these amino acids are heated with a strong alkali and lead acetate under alkaline conditions, it forms a black precipitate of lead sulfide. A positive result is indicated by the black precipitate, showing the presence of cysteine or cystine, while a negative result has no precipitate, showing their absence. The test helps distinguish sulfur from non-sulfur containing amino acids.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    166 views8 pages

    Lead Acetate Test GRP 7

    Uploaded by

    jeannikkav
    The lead acetate test is used to detect the presence of sulfur-containing amino acids like cysteine and cystine in a sample. When these amino acids are heated with a strong alkali and lead acetate under alkaline conditions, it forms a black precipitate of lead sulfide. A positive result is indicated by the black precipitate, showing the presence of cysteine or cystine, while a negative result has no precipitate, showing their absence. The test helps distinguish sulfur from non-sulfur containing amino acids.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 8

    Lead Acetate

    Test
    By GROUP 7 - Team Leo
    What is Lead Acetate Test?
    Also known as Lead Sulfide Test is a test specific for sulphur containing amino
    acids (Cysteine, Cystine, and Methionine). Even though the test is specific for the
    detection of sulfur-containing amino acids, methionine doesn’t give a positive
    result in this test.
    Objectives of lead
    Acetate Test:
    To detect the presence of sulfur-
    containing amino acids in a sample.
    To detect protein-containing cysteine
    and cystine in a given sample.
    To distinguish between sulfur-containing
    and non-sulfur containing amino acids.
    Principle of Lead
    Acetate Test
    The sulfur-containing amino acid such as cysteine, cysteine, and
    methionine (sulfhydryl/thiol group) reacts with lead acetate under
    alkaline conditions to form a brown precipitate.

    When cysteine is heated with strong alkali like NaOH, some of the
    sulphur is converted to sodium sulphide (Na2S) which can be detected
    by precipitation as lead sulphide (PbS) from alkaline solution.
    sulfur in cystine + 10% NaOH → heating→ Na2S
    Na2S + Pb (CH3COO)2 →(black precipitate) PbS
    Result and Interpretation of
    Lead Sulfide Test

    Positive test: A positive test in the Lead


    sulfide test is represented by the formation
    of black precipitate at the bottom of the test
    tube. This indicates the presence of cysteine
    or cystine in the solution.

    Negative test: A negative result in the Lead


    sulfide test is represented by the absence of
    Note: The addition of excess lead acetate to the black residue in the test tube. This indicates
    solution might result in white-colored precipitation.
    the absence of cysteine or cystine
    References:
    Sapkota, A. (2022, June 23). Lead Sulfide Test- Definition, Principle,
    Procedure, Result, Uses. Microbe Notes. https://microbenotes.com/lead-
    sulfide-test/

    Lead acetate test (Lead sulfide test): Principle, Reaction, Reagents,


    Procedure and Result Interpretation | Online Biochemistry Notes. (2020,
    April 16). Online Biochemistry Notes | Biochemistry Notes by Anup Basnet.
    http://biocheminfo.com/2020/04/16/lead-acetate-test-lead-sulfide-test-
    principle-reaction-reagents-procedure-and-result-interpretation/

    Karki, G. (2018, April 25). Lead sulfide test: Detection of amino acid
    containing sulfhydral group (-SH) - Online Biology Notes. Online Biology
    Notes. https://www.onlinebiologynotes.com/lead-sulfide-test-detection-
    of-amino-acid-containing-sulfhydral-group-sh/
    Thank
    you!

    You might also like