Chapter 4 Lesson 3
Chapter 4 Lesson 3
ACTIVITY
- Construct a flow chart showing the general process of producing GMOs or genetically
modified organisms.
1.Identify Desired Trait: The
first step is to identify the
specific trait that scientists
want to introduce or modify
in the target organism. This
trait can be related to
improved yield, resistance
to pests, tolerance to
environmental stress, or
other beneficial
characteristics.
2.Select Parental
Organisms: Researchers
choose the parent
organisms that possess the
desired trait or have the
potential to contribute to it.
These parent organisms will
serve as the source of the
genetic material to be
transferred.
3.Isolate Genes of Interest:
Scientists isolate the genes
responsible for the desired
trait from the DNA of the
parent organism. These
genes encode the specific
proteins or traits that are to
be Introduced into the
target organism.
4.Construct Recombinant
DNA: Recombinant DNA is
created by combining the
isolated genes with a vector,
often a plasmid or a virus.
The vector acts as a carrier
to deliver the genes into the
cells of the target organism.
5.Introduce Genes into
Target Organism: The
recombinant DNA
containing the desired
genes is introduced into the
cells of the target organism.
This can be done through
various techniques, such as
agrobacterium-mediated
transformation, gene gun,
or electroporation.
6.Incorporate Genes into
Genome: Once inside the
target organism’s cells, the
introduced genes become
part of the organism’s
genome. The genes will be
replicated and expressed
along with the organism’s
own genetic material.
7.Selection and Screening:
The transformed cells are
selected and screened to
identify those that have
successfully incorporated
the desired genes. Various
markers are used to identify
the transformed cells.
8.Regeneration: In the case
of plants, the transformed
cells are regenerated into
whole plants. This step
involves tissue culture
techniques and optimizing
conditions for growth.
9.Phenotypic and Molecular
Analysis: The developed
organisms are analyzed for
the expression of the
introduced trait at both the
molecular and phenotypic
levels to ensure the desired
changes are present.
10.Testing and Evaluation:
The GMOs undergo rigorous
testing and evaluation for
safety, efficacy, and
environmental impact. This
step includes laboratory
studies, greenhouse trials,
and field trials.
11.Regulatory Approval:
Depending on the country,
GMOs must undergo
regulatory scrutiny and
approval before they can be
commercially released or
used in agriculture.
ASSESSMENT
Try to envision the future of GMOs. If you were a certified scientist who can actually produce
GMOs, design a GMO with desirable characteristics that could be of great help to the society.
Sketch your GMOs inside the box below and describe it.
This is the first sketch that I will do, considering also this 6 steps
5. Gene Expression:
Add another arrow from the modified gene location to a nearby location outside the nucleus.
This represents the process of gene expression, where the inserted gene’s information is used to
produce specific proteins or traits in the organism.
6. Trait Expression:
Finally, draw an arrow from the gene expression location to the overall organism to indicate the
expression of the desired trait in the GMO. This trait could be enhanced yield, pest resistance,
or any other characteristic that the GMO has been engineered to possess.