Sustrato (mM) Velocidad (mMol/min) 1/mM 1/V

3 10.4 0.333 0.096

5 14.5 0.200 0.069
10 22.5 0.100 0.044
30 33.8 0.033 0.030
90 40.5 0.011 0.025

Cinética Enzimática
45
40
35
30
V mM/ min

25
20
15
10
5
0
0 10 20 30 40 50 60 70 80 90 100
[S] uM

Column C
 Línea Inversa
0.120

0.100
f(x) = 0.223988316800025 x + 0.0224053322144047
0.080 R² = 0.998814996980906
1/mM

0.060

0.040

0.020

0.000
80 90 100 0.000 0.050 0.100 0.150 0.200 0.250 0.300 0.350
Axis Title

Column E Linear (Column E)

 Velocidad sin Inhibidor (mMol/min) 1/mM 1/V
0.71 2 1.4085
1.07 1 0.9346
1.5 0.5 0.6667
1.8 0.2857 0.5556
1.88 0.2 0.5319

Cinética enzimatica
1.6
Velocidad sin Inhibidor (mMol/min)

2
1.8 1.4
f(x) = 0.4939444
1.6 1.2 R² = 0.99913031
1.4 1
1.2

1/mM
0.8
1
0.8 0.6
0.6 0.4
0.4 0.2
0.2
0
0 0 0.5
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Concentración M (10-4)

Column B
 Línea inversa
1.6
1.4
f(x) = 0.493944489191598 x + 0.425717089885809
1.2 R² = 0.999130312508754
1
1/mM

0.8
0.6
0.4
0.2
0
0 0.5 1 1.5 2 2.5
1/V

Column D Linear (Column D)

 Concentracion M (10-4) Velocidad con Inhibidor (mMol/min) 1/mM
0.5 0.43 2
1 0.71 1
2 1.05 0.5
3.5 1.41 0.2857
5 1.6 0.2

Cinética enzimatica
1.8 2.5
1.6 f(x) = 0.940629705
2 R² = 0.9992020775
1.4
1.2 1.5

1/ mM
1 1
0.8
0.5
0.6
0.4 0
0 0.5
0.2
0
0 1 2 3 4 5 6

Column O Colu
 1/V
2.3256
1.4085
0.9524
0.7092
0.625

Linea inversa
2.5
f(x) = 0.940629705375399 x + 0.454326436657055
2 R² = 0.999202077569824

1.5
1/ mM

0.5

0
0 0.5 1 1.5 2 2.5
1/V

Column R Linear (Column R)

Concentracion (mM/L) Velocidad sin Inhibidor (mg/min) 1/mM 1/V
1.5 0.21 0.6667 4.7619
2 0.25 0.5 4
3 0.28 0.3333 3.5714
4 0.33 0.25 3.0303
8 0.44 0.125 2.2727
16 0.43 0.0625 2.3256

Cinetica enzimática L
0.5
5
0.45
4.5 f(x) = 4.22238943238182
0.4 R² = 0.978290080223525
4
0.35
3.5
0.3
3
0.25
2.5
0.2
2
0.15
1.5
0.1
1
0.05
0.5
0
0 2 4 6 8 10 12 14 16 18 0
0 0.1 0.2
Column B
Co
 Cinetica
Línea inversa
0.2
0.18
f(x) = 4.22238943238182 x + 1.96350341246004 0.16
R² = 0.978290080223525
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
0 2 4 6 8
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

Column F Linear (Column F)

 Concentracion (mM/L) Velocidad con Inhibidor (mg/min) 1/mM
1.5 0.09 0.6667
2 0.1 0.5
3 0.12 0.3333
4 0.13 0.25
8 0.16 0.125
16 0.18 0.0625

Cinetica Enzimatica L
0.2 12
0.18 f(x) = 9.61730616689917 x +
0.16 10 R² = 0.9962963337322

0.14
8
0.12
0.1 6
0.08
0.06 4
0.04
2
0.02
0 0
0 2 4 6 8 10 12 14 16 18 0 0.1 0.2 0.3

Column P Colum
 1/V
11.3636
10
8.3333
7.6923
6.25
5.5556

Linea Inversa

f(x) = 9.61730616689917 x + 5.09354488360548

R² = 0.9962963337322

0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

Column U Linear (Column U)