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    Genetics Laboratory 1 and 2

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    cherrylynmanzano
    This document provides instructions for a lab experiment examining onion and cheek cells under a microscope. For the onion cell lab, students prepare a wet mount slide of onion skin and observe the cells under low and high power magnification both with and without iodine staining. They document their observations through drawings and answer questions about cell structure. For the cheek cell lab, students prepare a slide by scraping the inside of their cheek and staining with methylene blue. They observe the cheek cells under different magnifications and document their observations through drawings and responses to conclusion questions about cell structure and function.

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    © All Rights Reserved
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    Genetics Laboratory 1 and 2

    Uploaded by

    cherrylynmanzano
    21 views7 pages
    This document provides instructions for a lab experiment examining onion and cheek cells under a microscope. For the onion cell lab, students prepare a wet mount slide of onion skin and observe the cells under low and high power magnification both with and without iodine staining. They document their observations through drawings and answer questions about cell structure. For the cheek cell lab, students prepare a slide by scraping the inside of their cheek and staining with methylene blue. They observe the cheek cells under different magnifications and document their observations through drawings and responses to conclusion questions about cell structure and function.

    Original Description:

    Animal and Plant Cell Observation

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    This document provides instructions for a lab experiment examining onion and cheek cells under a microscope. For the onion cell lab, students prepare a wet mount slide of onion skin and observe the cells under low and high power magnification both with and without iodine staining. They document their observations through drawings and answer questions about cell structure. For the cheek cell lab, students prepare a slide by scraping the inside of their cheek and staining with methylene blue. They observe the cheek cells under different magnifications and document their observations through drawings and responses to conclusion questions about cell structure and function.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    21 views7 pages

    Genetics Laboratory 1 and 2

    Uploaded by

    cherrylynmanzano
    This document provides instructions for a lab experiment examining onion and cheek cells under a microscope. For the onion cell lab, students prepare a wet mount slide of onion skin and observe the cells under low and high power magnification both with and without iodine staining. They document their observations through drawings and answer questions about cell structure. For the cheek cell lab, students prepare a slide by scraping the inside of their cheek and staining with methylene blue. They observe the cheek cells under different magnifications and document their observations through drawings and responses to conclusion questions about cell structure and function.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 7

    PRINCIPLES IN GENETICS GEN211

    Course/Block: ______________________ Date: / __/ __ /


    Member:
    1. _____________________
    2. _____________________
    3. _____________________
    4. _____________________
    5. _____________________
    6. _____________________
    7. _____________________

    Lab #1: The Onion Cell


    Materials (What exactly do you need to do this experiment?)
    Whole red onion Iodine/Gram Stain Kit
    Microscopes Dropper
    Microscope slides Cover slips
    Method/Procedure (A list of all the steps that you take to do this experiment)

    Preparing the wet mount slide of onion skin


    1. Break an onion slice in two.
    2. Carefully pull the slice apart.
    3. Use tweezers to pull off a very thin piece of onion
    skin (the thinner, the better)
    4. Place the skin in the center of the slide. Try to keep
    it from folding; get it as flat as possible.
    5. Add a drop of water to the onion skin and cover with
    a cover slip.
    6. Press the cover slip down carefully to remove any air
    bubbles.
    7. Place the slide on the stage of the microscope, set it
    to low power, adjust the focus so the onion slice is
    clear. Draw four or five cells as seen. Label the cell
    walls.
    8. Switch to higher power and try to identify the cell
    membrane, nucleus, and cytoplasm.

    Staining the onion cells


    1. Lift up the cover slip and add one or two drops of
    iodine/Gram Stain to the slide.
    2. Lower the cover slip and examine the cells on higher
    power.
    3. Iodine stains different parts of the cells so that
    different organelles can be seen. Draw and label the
    cell wall, cell membrane, cytoplasm, and nucleus.

    Observations (What do you see in words?)


    Onion Cell Drawings

    Remember:
    Use pencil only
    No sketchy lines
    No shading
    Include magnification

    Conclusions & Discussion


    1. What is the general shape of the onion cells?

    2. Describe what you saw without the stain.

    3. Why is it easier to see the onion cells after they are stained?

    4. All plant cells have cell walls. What is the function of the cell wall?

    5. Count the number of cells that are seen in the field of view under low power magnification and
    also under higher magnification. Compare the number of cells observed in each field of view.
    What difference do you see?

    6. What is the job of the nucleus? Were you able to see the cell nuclei?

    7. What would you do differently if you were to do this experiment again? What mistakes will you
    make?
    Lab #2: Cheek Cell
    Materials (What exactly do you need to do this experiment?)

    Sterilised toothpick , watch glass, slide, coverslip, needle, forceps, brush dropper, water, glycerine,
    methylene-blue stain, microscope, blotting/filter paper,

    Method/Procedure (A list of all the steps that you take to do this experiment)

    1. Put a drop of methylene blue on a slide. Caution: methylene blue will stain clothes and
    skin.
    2. Gently scrape the inside of your cheek with the flat side of a toothpick. Scrape lightly.
    3. Stir the end of the toothpick in the stain and throw the toothpick away.
    4. Place a coverslip onto the slide.
    5. Use the SCANNING objective first and use the coarse adjustment knob to bring the slide
    into focus. Cells will appear very tiny at this magnification.
    6. Switch to LOW POWER and use the coarse adjustment knob. Cells should be visible, but
    they will be small and look like nearly clear purplish blobs. If you are looking at something
    very dark purple, it is probably not a cell.
    7. Once you think you have located a cell, switch to HIGH POWER and focus using the fine
    adjustment knob. (Remember, do NOT use the coarse adjustment knob at this point.)
    Observations (What do you see in words?)
    Cheek Cell Drawings

    Remember:
    Use pencil only
    No sketchy lines
    No shading
    Include magnification

    Conclusions & Discussion


    1. Why is methylene blue necessary?

    2. The light microscope used in the lab is not powerful enough to view other organelles in the
    cheek cell. What parts of the cell were visible?

    3. List 2 organelles that were NOT visible but could be found in cells if you had a microscope
    with a better magnification.

    4. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your saliva
    starts the process of breaking down the food you eat. Keeping this in mind, what organelle do
    you think would be numerous inside the cells of your mouth?

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