Dresscher & Mark. 1976

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Hydrobiologia vol. 48, 3, pag .

199-201, 1976

A SIMPLIFIED METHOD FOR THE BIOLOGICAL ASSESSMENT OF THE QUALITY OF


FRESH AND SLIGHTLY BRACKISH WATER

Th . G . N . DRESSCHER* & H . van der MARK**

*Prinses Marijkelaan i, Wezep . The Netherlands


**Roerstraat 5, Emmeloord . The Netherlands

Received April 22, 1974

Keywords : Saprobic index. Biological quality assessment

Abstract mented and modified by Liebmann (1962), Slade6ek


(1963) and other workers . The two first mentioned re-
A simplified method for the biological assessment of the searchers called their method for the determination of the
saprobic quality of surface water is described . The method degree of pollution 'Saprobiensystem' . Although this
has the advantage that it is unnecessery to determine accu- system still lacks a fundamental basis, it is in fact used in
rately the species of organisms present in the sample, many forms for the assessment of the quality of water .
which must be done when existing saprobic systems are
used . The method described here cannot be used when
there is a great abundance of a single species and a very Description of the simplified method
strong environmental disturbance must be assumed .
The above-mentioned evaluation systems require a thor-
ough knowledge of the organisms present in plankton . W e
Introduction have, however, assumed that the saprobic degree may also
be determined by examining the proportion between the
Over the years a number of systems have been designed numbers of species of single groups of microorganisms,
to assess the pollution of fresh water (Bick, 1963 ; Slade6ek, in other words, that the extent of pollution may manifest
1973), including the various saprobic systems in which the itself biologically in the numerical proportion between the
degree of water pollution is expressed in terms of saprobic heterotrophic, mixotrophic and autotrophic organisms
degrees . present in surface water at a given moment . It was for this
The saprobic degree is a measure for the phase in which reason that we chose the following groups :
the process of conversion of biologically decomposable Ciliata, indicating polysaprobity group A
substances has taken place in surface water . Determina- Euglenophyta, indicating a-mesosaprobity group B
tion of these phases, which merge imperceptibly into one Chlorococcales + Diatomeae, indicating
another, can be made by examining the population of 13-mesosaprobity group C
living organisms present in the water, since experience has Peridineae + Chrysophyceae + Conjugatae,
shown that some organisms are able to survive in a partic- indicating oligosaprobity group D
ular phase of the conversion process . The organisms that
more or less clearly restrict themselves to a certain phase To determine the numerical proportion between the spe-
are used to mark that phase . Kolkwitz & Marsson (i9o8, cies found within these groups, we began by using plankton
1909) listed groups of organisms, which were later supple- material concentrated from one or more litres of water .
The number of species of Ciliata may only be determined
on the day when samples are taken and in unfixed samples
Dr . W. Junk b .v. Publishers - The Hague, The Netherlands of water, i .e . with living organisms . In other cases, micro-

199
scopic examination may be carried out on fixed material . tem . Experience has shown that the number of these spe-
The number of forms is recorded for each group . The cies has no significant effect on the results obtained with
proportion between the numbers found constitutes the the formula recommended . For instance, figure r gives
basis for an assessment . When one group clearly predom- data on net plankton recorded at Lobith (The Nether-
inates, the saprobic degree may be stated at once . lands) between the years 1968 and 1971 (Government
In view of the fact that the indicating importance of the Institute for Sewage Purification, unpublished data) pro-
groups are not quite the same and the overall picture of the cessed by the assessment method proposed by the authors
figures more clearly reflects the transition from one phase and the widely used Pantle & Buck method (1955) . The
to another, a formula has been devised to simplify assess- similarity of the results obtained with the two systems is
ment and to give the possibility to represent the results very clear . It should be pointed out, however, that our
graphically: method cannot be used when there is a great abundance
C+3D-B-3A of a single species ('water bloom') which virtually elimi-
X = ; this gives a single figure, the sa- nates the other species . In such cases we must assume that
A+ B +C+ D we are dealing with a very strong environmental distur-
probic quotient X . The letters indicate the number of bance and, as we stated above, the saprobic quotient
forms found for each group . The limits for this formula cannot be determined .
are - 3 (polysaprobic) to +3 (oligosaprobic) . Zero is there-
fore at the borderline between the situation a-meso-
saprobic//3-mesosaprobic and /3-mesosaprobic/a-meso- References
saprobic . This method has the advantage that an accurate
species determination, which is otherwise necessary for a Bick, H . 1963 . A review of central European methods for the bio-
logical estimation of water pollution levels .-Bull. World
biological analysis, is not compulsory . Classification of Health Org . 29 : 401-413 .
forms into the groups and counting the forms per group Kolkwitz, R. & M . Marsson. 19o8 . Oekologie der pflanzlichen
is sufficient . Table 1, may be useful for assessing water Saprobien .-Ber . Deutsch . Bot . Ges . 26a : 505-519 .
quality . In this table the various saprobic quotients are Kolkwitz, R . & M . Marsson. 1909 . Oekologie der tierischen
Saprobien .-Int . Revue Ges . Hydrobiol. Hydrogr . 2 : 126-152 .
compared with the degree of pollution and the saprobic Liebmann, H . 1962 . Handbuch der Frischwasser- und Abwasser-
phases . Biologie, Band I . Oldenbourg, MUnchen : 1-588 .
Pantle, R . & H . Buck. 1955 . Die biologische Ueberwachung der
Gewasser and die Darstellung der Ergebnisse . -Gas-, Wasser-
fach 96 : 604 .
Discussion Sladedek, V . 1963 . A guide to limnosaprobical organisms, -Sci .
Papers Inst . Technol . Prague, Technol . Water 7 : 543-612
Certain species of organisms which do not belong to the Sladedek, V . 1973 . System of water quality from the biological
saprobic systems will be found in the groups of our sys- point of view .-Ergebn. Limnol . 7 : 1-218 .

Table i .

load pollution saprobic phases saprobic quotient

many organic very severe polysaprobic -3 / -2


substances poly/a-mesosaprobic -2 / -1'/2

considerable a-meso/polysaprobic -1'/2 / -I


a-mesosaprobic -I / - '/2

organic and moderate a//3-mesosaprobic - '/2 / 0


inorganic substances /3/ a-mesosaprobic 0 / + /' 2

slight /3-mesosaprobic + '/2 / +1


/3-meso/oligosaprobic +1 / +1'/2

few organic and very slight oligo//3-mesosaprobic +1'/2 / +2


inorganic substances oligosaprobic +2 / +3

200
WATEROUALITY OF THE RIVER RHINE 1968-1971 SA PLING POINT LOBITH
OLIGOSAPROBI C

P-MESOSAPROBIC
V
4 -o--a -d

G-MESOSAPROBIC
A

POLYSAPROBIC
1968 I 1969 1 1970 1 1971

B S I = SYSTEM PANTLE & BUCK


0---o---0 = SYSTEM DRESSCHER 6 VAN DER MARK

FIGURE I

20 1

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