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Problema 7 y 8

Escherichia coli are grown aerobically in batch culture with glucose to produce recombinant porcine growth hormone. Cell and substrate concentrations are measured over time, with the maximum growth rate (μmax) calculated from this data. The observed biomass yield from substrate (Y'XS) is determined and whether it remains constant is examined. Similarly, Atropa belladonna hairy roots are grown in batch culture. Biomass and sugar concentrations are measured periodically and used to analyze the maximum growth rate, specific sugar uptake rate, and observed biomass yield from sugar (Y'XS) and whether it is constant.

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Angie Ramirez
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249 views1 page

Problema 7 y 8

Escherichia coli are grown aerobically in batch culture with glucose to produce recombinant porcine growth hormone. Cell and substrate concentrations are measured over time, with the maximum growth rate (μmax) calculated from this data. The observed biomass yield from substrate (Y'XS) is determined and whether it remains constant is examined. Similarly, Atropa belladonna hairy roots are grown in batch culture. Biomass and sugar concentrations are measured periodically and used to analyze the maximum growth rate, specific sugar uptake rate, and observed biomass yield from sugar (Y'XS) and whether it is constant.

Uploaded by

Angie Ramirez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Growth parameters for recombinant E.

coli
Escherichia coli is being used for production of recombinant porcine growth hormone. The bacteria are
grown aerobically in batch culture with glucose as growth-limiting substrate. Cell and substrate
concentrations are measured as a function of culture time; the results are listed below.

Time Cell concentration, x Substrate concentration, s


(h) (kg/m3) (kg/m3)

0.0 0.20 25.0


0.33 0.21 24.8
0.5 0.22 24.8
0.75 0.32 24.6
1.0 0.47 24.3
1.5 1.00 23.3
2.0 2.10 20.7
2.5 4.42 15.7
2.8 6.9 10.2
3.0 9.4 5.2
3.1 10.9 1.65
3.2 11.6 0.2
3.5 11.7 0.0
3.7 11.6 0.0

(a) Plot 𝜇𝜇 a as a function of time.


(b) What is the value of 𝜇𝜇𝑚𝑚𝑚𝑚𝑚𝑚 ?
(C) What is the observed biomass yield from substrate? Is 𝑌𝑌′𝑋𝑋𝑋𝑋
constant?

Growth parameters for hairy roots


Hairy roots are produced by genetic transformation of plants using Agrobacterium rhizogenes. The following
biomass and sugar concentrations were obtained during batch culture of Atropa belladonna hairy roots in a
bubble-column fermenter.

Time, (d) Biomass concentration Sugar concentration


(g /l dry weight) (g /1)
0 0.64 30.0
5 1.95 27.4
10 4.21 23.6
15 5.54 21.0
20 6.98 18.4
25 9.50 14.8
30 10.3 13.3
35 12.0 9.7
40 12.7 8.0
45 13.1 6.8
50 13.5 5.7
55 13.7 5.1

(a) Plot 𝜇𝜇 as a function of culture time. When is the growth rate maximum?
(b) Plot the specific rate of sugar uptake as a function of time.
(c) What is the observed biomass yield from substrate? Is 𝑌𝑌′𝑋𝑋𝑋𝑋 constant?

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