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GPC QuickGuide

1. The document describes the process of creating a calibration curve for gel permeation chromatography (GPC) using Shimadzu's LabSolutions GPC software. 2. The process involves adding standard sample data files, setting peak integration and GPC calculation parameters, and generating a calibration curve that is saved in a method file. 3. Once a calibration curve has been created, it can be used to perform GPC calculations and analyze unknown samples, with results that can be viewed in the GPC Data Analysis and GPC Data Comparison windows.

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Hamza Mohammed
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151 views

GPC QuickGuide

1. The document describes the process of creating a calibration curve for gel permeation chromatography (GPC) using Shimadzu's LabSolutions GPC software. 2. The process involves adding standard sample data files, setting peak integration and GPC calculation parameters, and generating a calibration curve that is saved in a method file. 3. Once a calibration curve has been created, it can be used to perform GPC calculations and analyze unknown samples, with results that can be viewed in the GPC Data Analysis and GPC Data Comparison windows.

Uploaded by

Hamza Mohammed
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 14

223-60672A

Nov. 2015

Shimadzu High Performance Liquid Chromatography

LabSolutions GPC

Quick Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
© 2014-2015 Shimadzu Corporation. All rights reserved.
Original version is approved in English.
1 Flow of Analysis Operation

1 Flow of Analysis Operation


In this chapter, the operation flow of LabSolutions GPC is explained as shown in the following chart.
In LabSolutions GPC, adding to the parameters for LabSolutions, you set parameters for calibration curve,
molecular weight calculation, etc. in a method file. You can create calibration curves in the [GPC Postrun]
program, and once you have set all these parameters, you can perform GPC calculation, such as
molecular weight calculation, while performing data acquisition by using the method file. This Quick
Manual describes the procedure for creating a calibration curve, a process inherent to GPC. For other
operations, refer to the "LabSolutions Operation Manual" or the "LabSolutions GPC Instruction Manual".

Setting the analysis conditions


1
LabSolutions
[Realtime Analysis] - [Data Acquisition]
LabSolutions Method file (.lcm)

Analyzing standard sample


LabSolutions [Realtime Analysis] - [Data
Acquisition] or [Batch Processing]
LabSolutions Data file (.lcd), Batch file (.lcb)

Setting peak integration parameters


[GPC Postrun] - [GPC Data Analysis]
Modifying the method in the data file (.lcd)
Can modify the method file (.lcm)
Creating reports
accordingly
[GPC Postrun] - [Report Generator]
Creates a report format file (.lsr) for GPC
Creating calibration curves Postrun data.
[GPC Postrun] - [GPC Calibration Curve]
Creates a calibration curve in a method file
(.lcm) using data files (.lcd) of standard
samples.

Analyzing unknown sample


LabSolutions [Realtime Analysis] - [Data
Acquisition] or [Batch Processing]
Data file (.lcd), and Batch file (.lcb)

Viewing results
[GPC Postrun] - [GPC Data Analysis], and
[GPC Data Comparison]
Data file (.lcd)

Reanalyzing the results


[GPC Postrun] - [GPC Data Analysis] or
[Batch Processing]
Data file (.lcd)

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2 Creating Calibration Curve
 Opening a File for Editing

1 Display the [LabSolutions Main] window.

2 Click (Postrun).

3 Double-click (GPC Postrun).

The [GPC Postrun] program will start.

4
2 Creating Calibration Curve

4 Click (GPC Calibration Curve) from the [Main] assistant bar in the [GPC
Postrun] program.

The [GPC Calibration Curve] window appears.

5 Click [Open Method File] from the [File] menu.

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6 Select the method file and click [Open].

NOTE
You can select either LabSolutions method files or those from older software versions.

 Adding Data Files for Use in Creating a Calibration Curve

1 Click [Data File List] from the [Data] menu.

2 Click [Add].

6
2 Creating Calibration Curve

3 Select the standard sample data you wish to use for creating calibration curve, and click
[Open].

4 Verify that the data file of the standard sample is displayed in the data file list, and click
[OK].

5 Click [Edit] in the [Calibration Table View] screen.

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6 Click [Pick up] in the [Calibration Table View] screen.

7 Click [All peaks] or [Select peaks].


Select [All peaks] when importing data of the whole retention time, and select [Select peaks] when
importing data for a certain time range. When [Select peaks] is selected, use the mouse to specify the
range on the chromatogram.

8 Click [New] or [Add].


Select [New] when importing peak data after deleting an existing calibration curve. Select [Add] when
adding peak data to existing calibration points.

8
2 Creating Calibration Curve

9 Set the parameters not yet defined in the calibration curve table, such as molecular
weight values.

9
 Setting Data Processing Parameters

1 Select [Data Processing Parameters] from the [Method] menu.

2 Click [Advanced] in the [Integration] tab.

3 Enter the number of slices in [Max.Slices].


Set the maximum value: [50000].

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2 Creating Calibration Curve

4 Check [GPC Calculation] in the [GPC Calculation] tab.

5 Select the [Curve] type in the [GPC Calibration Curve] tab, and click [OK].

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6 Verify the calibration curve created in the [Calibration Curve View] screen.

NOTE
To modify the calibration curve, configure the settings again in the [Data Processing Parameters] -
[GPC Calibration Curve] tab.

7 Click [Save Method File] from the [File] menu.

The calibration curve is saved in the method file.

A method file with the calibration curve configured has been created. By using this method file to analyze
an unknown sample, GPC calculations can be performed. (This can also be carried out online in
conjunction with data acquisition.)

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3 Checking the Results

3 Checking the Results


The GPC calculation results can be checked by opening the data file from the [GPC Data Analysis] window
or the [GPC Data Comparison] window.

 [GPC Data Analysis] window

To display the calculation results, select the appropriate item from the [View] menu.

1 Click the appropriate item from the [View] menu.

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2 Click the appropriate tab in the [Result Display] screen to check the contents.

 [GPC Data Comparison] window

Multiple data results can be checked in this window.

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