File No.

AHF02/12/2021-EPIDEMOLOGY SEC-DAH

GOVERNMENT OF ANDHRA PRADESH

DIRECTORATE OF ANIMAL HUSBANDRY::A.P::VIJAYAWADA
Cir.Memo.Roc.No.AHF02/12/2021-EPID SEC-DAH,Dt:31.10.2023

Sub Animal Husbandry Department–Brucella Vaccination Programme III rd

: round under National Animal Disease Control Programme (NADCP)-
Phase II - Scheduled from 10.11.2023 to 30-11-2023 in all districts
in the state–Targets and Sero monitoring study plan recommended by
NIVEDI, Bengaluru for implementation- Guidelines for implementation–
Issued–Regarding.
Ref: 1. T.O Memo.roc.no.AHF02/12/2021/2-EPID SEC-DAH,Dated:20-06-
2023.
2. PME NUMBER: F.NO.13/NIVEDI/PMEC/EM & TB/2021-22/2324-01.
3. T.O. Cir.Memo.Roc.No.AHF02/12/2021-EPID SEC-DAH,
Dt:26.06.2023 &
21.07.2023
*****
The attention of the DAHOs of all districts in the state is invited to
the subject & references cited and informed that the Second Phase of
Brucellosis Vaccination programme under NADCP in the FY 2023-24 is
scheduled from 10.11.2023 to 30-11-2023 for which the vaccine,
logistics are already positioned at divisional level. The targets for Phase II
Brucella Control Programme are as below.

Total
Calves
Targets Female Targets
Born from Cumulative
S.No District for Calves for
Apr'2023 Target
Phase I @50% Phase II
to
July'2023
1 Srikakulam 28747 30830 15415 13874 42621
2 Vizianagaram 23998 27330 13665 12299 36297
Parvathipuram
6751
3 Manyam 8370 4185 3767 10518
Alluri Sitarama
10430
4 Raju 451 226 203 10633
5 Visakhapatanam 4633 5381 2691 2421 7054
6 Anakapalli 22511 22390 11195 10076 32587
7 Kakinada 11486 14021 7011 6309 17795
Dr.B.R.Ambedkar
11291
8 Konaseema 13228 6614 5953 17244
9 East Godavari 12538 10437 5219 4697 17235
10 West Godavari 13653 16092 8046 7241 20894
11 Eluru 24354 21596 10798 9718 34072
12 Krishna 24205 21022 10511 9460 33665
13 NTR 16490 14349 7175 6457 22947
14 Guntur 16250 16773 8387 7548 23798
15 Bapatla 21597 19529 9765 8788 30385
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16 Palnadu 21946 19376 9688 8719 30665

17 Prakasam 22045 25484 12742 11468 33513
18 SPSR Nellore 20210 20872 10436 9392 29602
19 Tirupati 17743 25066 12533 11280 29023
20 Chittoor 41502 51314 25657 23091 64593
21 Annamayya 20986 28680 14340 12906 33892
22 YSR 14816 16919 8460 7614 22430
23 Sri Satyasai 14217 20280 10140 9126 23343
24 Anantapur 9046 11366 5683 5115 14161
25 Kurnool 9628 10827 5414 4872 14500
26 Nandyal 14241 15324 7662 6896 21137
Grand Total 455314 487307 243654 219290 674604

All the DAHOs are requested to implement the Brucellosis Control

Programme as per the guidelines enclosed as annexure.

Encl: as above

DIRECTOR

To,
All the District Animal Husbandry Ofcers for information and with a
request to communicate the same to the Divisional DDs (AH), ADs of I/c
Area VH , DD / AD I/c ADDL’s/RADDLs in the state.
The CEO, APLDA, Guntur with a request to communicate the same to the
Eos DLDAs in the state.
Copy to the Joint Director (AH), VBRI AP, Vijayawada for information &
necessary action.
Copy submitted to the Special chief Secretary to Government, Animal
Husbandry, DD & F Department, A.P. Secretariat, Velagapudi, Amaravathi.
Copy to SF / Spare
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ANNEXURE
BCP III rd ROUND (2023-24) – PHASE II - GUIDELINES
(Scheduled from 10-11-2023 to 30-11-2023)
The following guidelines are herewith communicated for efective
implementation of Brucella vaccination programme in the districts.
OBJECTIVES OF THE PROGRAMME: The overall aim of the
National Animal Disease Control Programme for Brucellosis (NADCP) is
to control Brucellosis by 2025 with vaccination and its eventual
eradication by 2030 by Vaccinating the 100% Bovine female calves (4-8
months) population of bovines once in a life time.
Major activities of the programme :
• Identification of target animals by ear-tagging, registration and
uploading the data in the animal health module of Bharat Pashudhan
Application ( Care should be taken, so that the vaccinated calf
details must be entered after entry of calf details in NAIP only, NAIP
entry date should be 4 months prior to vaccination date)

• Uploading the vaccination data in the Bharat Pashudhan

• Maintaining record of vaccination through Animal Health cards.

• Post vaccination Sero monitoring of animal population.

• Recording/Regulation of animal movement through temporary

quarantine/ check- posts

• Regular monitoring by Departmental Ofcers.

• Evaluation through Telephonic Survey/CALL CENTRE to measure the

impact of the programme.

Guidelines:
• Prepare Village wise action plan along with the vaccination teams
before start of the program.

• One needle shall be used for one animal one syringe for 5 calves
may be used. Logistics supplied during previous round should be
utilized for this purpose.

• Vaccinate 100% bovine female calves (4-8 months) through S/C

route with 2ml dose once in a life time.

• Deworming of calves is mandatory before vaccination.

• Ensure cold chain (2-8o C) is maintained throughout the programme.

During the vaccination campaign, it should be ensured that vaccine
bottles are continuously kept in vaccine carriers so that cold- chain is
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maintained.

• Vaccination program shall start by 6.30 AM on the scheduled dates.

• Empty vaccine vials shall be handed over to concerned AVH Ads.

• Vaccinators shall take care to avoid spillage of vaccine during filling

up the syringe.

• Wastage of vaccine should be minimized and it should not be more

than 2-3% under any circumstances.

• Vaccinators shall ensure that vaccine bottles once opened

(punctured) should be used on the same day itself to avoid
deterioration of potency / quality of vaccine.

• Before opening the vaccine bottle it shall be ensured that availability

of sufcient number of animals for vaccination to ensure maximum
utilization of vaccine. Un-necessary discard of vaccine should be
discouraged.

• Brucella vaccination shall be conducted as per the 20 th Quinquennial

livestock census.

• Vaccinator shall hand over the vaccination card, duly signed by the
Veterinary Ofcer, to every animal owner after vaccinating the
particular animal.

• All vaccinators/Team members shall register in Bharat Application

app.

• All vaccinations shall be entered in Bharat Application application.

• False reporting of vaccination should be avoided. If any false

reporting is resorted to, disciplinary action will be initiated.

PRECAUTIONS:
• The dose of vaccine used shall be as per the manufacturer’s
instructions which are at present 2 ml for each calf.

• Route — subcutaneous (Care must be taken not to rupture bigger

blood vessels that may cause emboli in the blood stream).

• Brucella vaccine shall be stored under temperature between 2°C and

8°C .

• The vaccine shall neither be frozen nor be exposed to temperature

higher than 8°C.

• The teams should carry emergency / supportive medicines with

them (to meet emergencies if any).

• Usage of vaccine before the expiry date should be ensured.

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• Proper sterilization procedure & proper cold chain precautionary

measures have to be maintained while conducting Brucella
Vaccinations.

• While collection of serum sample, use only disposable needles and

proper sterilization of the site of collection & maintain proper cold
chain from collection to the storage of the samples.

• Reconstitute the freez dried vaccine with given 10 ml diluent and

vaccinate (5) calves, Dose: 2 ml, Route: Sub cutaneously.

• Use diluents provided with the vaccine for re constitution.

Functions of the District Monitoring Unit:-

District monitoring unit shall be constituted with the following members.
1. Collector and District Magistrate - Chairman

2. District Animal Husbandry Ofcer - Member / Convener

3. Divisional Deputy Directors - Members

4. Deputy / Assistant Directors, I/c ADDLs - Member

• The DMUs shall monitor and co-ordinate with field level staf and
ensure implementation of all the above activities in their respective
Districts.

• The DMU shall be the main executive unit in the entire

implementation of the programme in the District. DMU shall be
responsible in ensuring the required vaccines and all logistics
required for vaccination including man-power for carrying out
vaccination, cold chain cabinets and vaccine carriers are in place
well before the start of the vaccination programme.
• DMU shall ensure execution of vaccination in the entire district as
per schedule and prescribed procedures, collection of sera samples
for sero-monitoring, animal identification and documentation.

• The DMU shall be responsible for surveillance within the district

during the entire project period and shall investigate any suspected
outbreaks of Brucellosis and arrange for sending specimen for
laboratory confirmation.

• DMU shall be responsible for training of staf engaged in vaccination

programme well before the start of the programme and should
prepare calendar of operation with the help of District Animal
Husbandry ofcer and Block Ofcers.

• DMU shall ensure mass education / awareness campaign on

Brucellosis amongst the livestock farmers giving emphasis on
economic impact of the disease and benefits likely to accrue due to
preventive vaccination of their animals and timely reporting of the
disease in case of its suspicion/occurrence. All forms of print and
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electronic media to be considered while disseminating the

information.

• DMU shall ensure working of the cold room / cabinets required for
storage and cold chain maintenance of the vaccine at district head
quarter.

• In case of failure of electricity, generators shall be made available to

maintain the cold chain.

• At the time of supply of vaccine by suppliers at district level,

maintenance of cold chain for vaccine shall be ensured and
temperature monitor card and Vaccine Vial Monitors(VVMs) should
also be inspected.

• DMU shall supervise vaccination programme and provide all

necessary required infrastructure facilities like aprons, disposable
syringes, needles, biological waste deposit bags, transportation
arrangement, vaccine containers etc. Logistics supplied during
previous round should be utilized for this purpose.

• DMU shall maintain the bufer stock of vaccines as per the

requirement to ensure prompt delivery.

• DMU shall compile epidemiological information and data of

vaccination programme and various reports and submit to SMU as
per the schedule.

• DMU shall co-ordinate with the respective Disease Investigation

Laboratories to collect post-vaccination sera samples for
seromonitoring by ICAR/ICAR-DFMD laboratories, etc.

• For proper identification of vaccinated animals, DMU shall have ear-

tags, tagging machines, etc. readily available before start of
vaccination. DMU shall ensure that vaccinated animals are
necessarily ear-tagged and registered in the INAPH animal health
module database.

• DMU shall ensure training of technicians involved in the process of

ear-tagging, entry of details required and uploading in the data in
the Bharat Pashudhan server. Simultaneous recording details of
vaccinated animals in animal health cards may also be ensured.

• DMU shall be responsible for ‘Master’ creation as well as vaccination

camp creation at district level in the animal health module of Bharat
Pashudhan App.

• DMU shall liaise with other agencies, Co-operative department,

Panchayati Raj Institutions, women Self-Help groups and Youth
organizations for efective implementation of the programme.

• DMU shall supervise proper disposal of bio-medical waste generated

during vaccination programme
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• Conduct surprise visits in the field as per the action plan.

Responsibilities Divisional DDs:

• The Divisional DDs shall be the main executive unit in the entire
implementation of the programme in the Division. DDs shall be
responsible in ensuring the required vaccines and all logistics
required for vaccination including man-power for carrying out
vaccination, cold chain cabinets and vaccine carriers are in place
well before the start of the vaccination programme.

• Divisional DDs shall supervise 100% Brucella vaccination with

tagging of all eligible female valves in their jurisdiction. If any OBRs
reported in those concerned areas, due to insufcient coverage of
vaccination, action will be taken accordingly.

• All the Divisional Deputy Directors should coordinate with field staf,
in motivation of vaccinators, and deploy sufcient man power for
vaccination.

• Shall ensure availability of adequate vaccine stock as per eligible

animal population and ensure maintenance of cold chain & shall also
have knowledge of vaccine vial monitors (VVM) and how the same
are to be interpreted. This information should also be given to the
vaccinator.

• Shall ensure all pre-requisite of the vaccination programme like

trained manpower, vaccine carriers, syringes, needles, biological
waste deposit bags, personnel protective equipment (apron, hand
glove, plain protecting glass), ear- tags, ear-tagging machines,
transport of vaccine, etc., before starting actual vaccination in the
block.

• Conduct surprise visits in the field as per the action plan.

• Divisional DDs shall supervise proper disposal of bio-medical waste

generated during vaccination programme.

• Divisional DDs shall co-ordinate with the respective Disease

Investigation Laboratories to collect post-vaccination sera samples
for seromonitoring by ICAR/ICAR-DFMD laboratories, etc.

• At the time of supply of vaccine divisional level, maintenance of cold

chain for vaccine shall be ensured and temperature monitor card
and Vaccine Vial Monitors(VVMs) should also be inspected

• Divisional DDs shall ensure mass education / awareness campaign

on Brucellosis amongst the livestock farmers giving emphasis on
economic impact of the disease and benefits likely to accrue due to
preventive vaccination of their animals and timely reporting of the
disease in case of its suspicion/occurrence. All forms of print and
electronic media to be considered while disseminating the
information.
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• Divisional DDs shall ensure execution of vaccination in the entire

district as per schedule and prescribed procedures, collection of sera
samples for sero-monitoring, animal identification and
documentation.

Responsibilities of Block Monitoring Unit/Assistant Director

(AH) I/c AVH’s:
• Responsible for monitoring of vaccination program in their AVH
jurisdiction.

• Supervision and coordination of VAS’s and Vaccinators regarding

vaccine supply, Cold chain maintenance and other logistics in their
jurisdiction.

• Responsible for data purity in Bharat Pashudhan applications. Many

vaccinators are entering the invalid mobile numbers (i.e., wrong
mobile numbers, 9 digit mobile numbers, mobile numbers starting
with 9 and 9 zeros, multiple farmers with one mobile number and
same mobile number for vaccinator and farmer) which is hampering
the evaluation through telephonic survey. Vaccination details with
such false mobile numbers will be treated as “not vaccinated” and
total number of such vaccinations will not considered for
achievement of target.

• Responsible for online data entry of physical vaccinations done on

real time Basis.

• Shall ensure that separate disposable needle is used for vaccination

of each animal.

• Shall ensure that technicians involved in ear-tagging are aware of

the procedure and enter relevant details required as per the animal
health module of Bharat Pashudhan App.

• Shall ensure that animal health cards with recorded details of

vaccinated animals are issued to animal owners. Each animal shall
be issued a Vaccination card and shall ensure that these cards have
been handed over to the animal owner.

• Shall help in collecting post- vaccinated sera samples for laboratory

analysis in coordination with concerned ADDL.

• Shall provide publicity material at local level such as leafets,

pamphlets, posters etc., to village panchayat, cattle market
authorities etc.

• Shall take support of Block Development Ofcer, local leaders and

key person(s) in the area, women self-help groups, youth
organizations, etc., for efective implementation of the programme.

• Ensure that all the vaccinators including AHAs, Gopalamitras and

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Paravets may be imparted one day practical training on vaccinations

and online uploading of the data in Bharat Pashudhan App.

Responsibilities of Veterinary Assistant Surgeon:

• The Ofcer in-charge of Veterinary Institutes with his team shall
vaccinate all eligible animals as per programme norms.

• VAS, i/c. Concerned VD is the Team leader.

• Concerned Veterinary Assistant Surgeons I/c Veterinary Dispensary

shall prepare village wise micro action plan for completion of
Brucella vaccination program within 15 days basing on coverage of
100% bovine female calves (4-8 months age) population coverage.

• Instruct the AHAs to conduct door to door Survey in advance to

ascertain the actual population available and one to one motivation.

• Ensure that all the vaccinators including newly recruited AHAs,

Gopalamitras and Paravets registered in Bharat Pashudhan App.
• Guide the field staf to vaccinate the animal at proper site and
through proper route ( S/C care must be taken not to vaccinate the
rump region of animal)

• Guide the field staf/vaccinator to correct the data i.e., mobile

numbers of farmers and any instance of invalid numbers in Bharat
Pashudhan App will be viewed seriously.

• Empty vaccine vials should be handed over to concerned AVH ADs.

• The farmers shall be kept informed well in advance about the

Brucella Vaccination Programme in their respective villages without
giving scope for any criticism.
Sero-Monitoring:
• No Sero-surveillance/pre vaccination sample collection is required for
Brucella vaccination

• NIVEDI, Bengaluru has issued sero-monitoring plan for Brucellosis

Control Programme Second Round for which 145 villages are
selected for collection of 1794 post vaccination serum samples, No
need to collect Pre-vaccination samples.

• The district nodal ofcers shall ensure to follow the SOPs

communicated by GOI for collection of post vaccinations sera
samples, Animal identification and documentation.

• Ofcers nominated for each district shall take up surprise visits and
submit their detailed report to the Head Ofce about the
implementation of the programme.

• Concerned Assistant Directors I/c. AVH, VAS shall monitor the

vaccination programme personally in the villages where post sera
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samples are collected for sero conversion studies. Any laxity shall be
viewed seriously.

• Hence all the departmental staf are requested to scrupulously follow

the BCP guidelines both in 100% vaccination coverage & collection
of post vaccination sera samples.

• Sero-monitoring plan is enclosed herewith

The Deputy Director (AH) /Assistant Director (AH), Animal Disease

Diagnostic Laboratory of the concerned district will be the Coordinating
Ofcer for this Programme and he / she will coordinate post vaccination
Sera sample collection for sero monitoring studies.

The Joint Director (AH), VBRI, Vijayawada shall monitor entire

vaccination programme through surprise visits to all 26 districts of State .
Hence, the District Animal Husbandry Ofcers are requested to
follow the above guidelines / precautions including Government of India
guidelines scrupulously for efective implementation of Brucella
Vaccination Programme under NADCP and submit District consolidated
report after completion of vaccination Programme (Both Hard & soft copy),
along with photographs, press clippings & success stories without fail.