Shaqlawa Technical College, Erbil Polytechnic University

Medical Laboratory Technology Department

First Stage, First Semester
2023-2024
Pr. General Chemistry
Lecturer: Hardi Rafat Baqi (MSc. Biochemistry)
E-mail: [email protected]
&
Lecturer: Karwan Hasan Azeez (BSc. Chemistry- MSc. Student)
E-mail: [email protected]
Basic laboratory operations in chemistry (Volume,
Mass, and Temperature measurements)
Units of Measurement
• The metric system of weights and measures is used by scientists of all
fields, including chemists.
• This system uses the base 10 for measurements; for conversions,
measurements may be multiplied or divided by 10.

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Units of Measurement
• The measures of length, volume, mass, energy, and temperature are
used to evaluate our physical and chemical environment.
• The table below compares the metric system with the more recently
accepted SI system (International System of Units).
• The laboratory equipment associated with obtaining these measures
is also listed.

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Length: use of the meterstick (or
metric ruler)
• The meterstick is used to measure length.
• One side of the meterstick has its divisions in inches (in.) with
subdivisions in sixteenths of an inch; the other side is in centimeters
(cm) with subdivisions in millimeters (mm).
• Some useful conversion factors are listed below.

• The meterstick can normally measure to 0.001 m, 0.1 cm, or 1 mm.

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Length: use of the meterstick (or
metric ruler)
1- With your meterstick (or metric ruler), measure the length and width
of this laboratory manual.
2- Take the measurements in inches (to the nearest sixteenth of an
inch) and in centimeters (to the nearest 0.1 cm). Record your response
on the Report Sheet.
3- Convert the readings in cm to mm and m.
4- Calculate the area of the manual in in2, cm2, and mm2.

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Volume: use of a graduated cylinder, an
Erlenmeyer flask, a beaker, and a burette
• Volume in the metric system is expressed in liters (L) and
milliliters (mL).
• Another way of expressing milliliters is in cubic centimeters (cm3
or cc).
• Several conversion factors for volume measurements are listed
below:

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Volume: use of a graduated cylinder
• The graduated cylinder is a piece of glassware used for measuring the volume of a liquid.
• Graduated cylinders come in various sizes with different degrees of accuracy.
• A convenient size for this experiment is the 100-mL graduated cylinder.
• Note that this cylinder is marked in units of 1 mL; major divisions are of 10 mL and
subdivisions are of 1 mL.
• Estimates can be made to the nearest 0.1 mL.
• When a liquid is in the graduated cylinder, you will see that the level in the cylinder is
curved with the lowest point at the center.
• This is the meniscus, or the dividing line between liquid and air.
• When reading the meniscus for the volume, be sure to read the lowest point on the curve
and not the upper edge.
• To avoid errors in reading the meniscus, the eye’s line of sight must be perpendicular to
the scale.

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Volume: use of a graduated cylinder

In next steps, use the graduated cylinder to see how well

the marks on an Erlenmeyer flask and a beaker measure
the indicated volume.

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Volume: use of an Erlenmeyer flask
1- Take a 50-mL graduated Erlenmeyer flask and fill with water to
the 50 mL mark.
2- Transfer the water, completely and without spilling, to a 100-
mL graduated cylinder.
3- Record the volume on the Report Sheet to the nearest 0.1 mL;
convert to L.

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Volume: use of a beaker
1- Take a 50-mL graduated beaker, and fill with water to the 40-
mL mark.
2- Transfer the water, completely and without spilling, to a dry
100-mL graduated cylinder.
3- Record the volume on the Report Sheet to the nearest 0.1 mL;
convert to L.

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Volume: comparison of measurements
• What is the error in mL and in percent (%) for obtaining 50.0 mL
for the Erlenmeyer flask and 40.0 mL for the beaker?
• Which piece of glassware will give you a more accurate
measure of liquid: the graduated cylinder, the Erlenmeyer flask,
or the beaker?

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Volume: use of burette and graduated
pipette
• In Burette and Pipettes it is common to measure the volume
delivered.
• This means that two volume measurements will be made.
• The volume delivered is the difference in these two volumes,
Vfinal -Vinitial.
• Make multiple measurements of volumes using burette and
graduated pipette.

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Mass: use of the laboratory balance
• Mass measurements of objects are carried out with the laboratory
balance.
• Many types of balances are available for laboratory use.
• The proper choice of a balance depends upon what degree of
accuracy is needed for a measurement.
• The standard units of mass are the kilogram (kg) in the SI system
and the gram (g) in the metric system.
• Some conversion factors are listed below:

• Make multiple measurements of mass using digital balances in lab.

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Temperature: use of the thermometer
• Routine measurements of temperature are done with a
thermometer.
• Thermometers found in chemistry laboratories may use either
mercury
ο
or a colored fluid as the liquid, and degrees Celsius
( C) as the units of measurement.
• The fixedο reference points on this scale are theο freezing point of
water, 0 C, and the boiling point of water, 100 C.
• Between these two reference points, ο
the scale is divided into
100 units, with each unit equal to 1 C.
ο
• Temperature can be estimated to 0.1 C.

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Temperature: use of the thermometer
• Other thermometers use either the Fahrenheit (F) or the Kelvin
(K) temperature scale and use the same reference points, that
is, the freezing and boiling points of water.
• Conversion between the scales can be accomplished using the
formulas below.

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Temperature: use of the thermometer
1- Use the thermometer in your kit and record to the nearest 0.1 οC the
temperature of the laboratory at room temperature.
• Use the Report Sheet to record your results.
2- Record the temperature of boiling water.
• Set up a 250-mL beaker containing 100 mL water, and heat on a hot
plate until boiling.
• Hold the thermometer in the boiling water for 1 min. before reading
the temperature (be sure not to touch the sides of the beaker).
ο
• Using the Report Sheet, record your results to the nearest 0.1 C.

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Temperature: use of the thermometer
3- Record the temperature of ice water.
• Into a 250-mL beaker, add enough crushed ice to fill halfway.
• Add distilled water to the level of the ice.
• Stir the ice water gently with a glass rod for 1 min. (use caution;
be careful not to hit the walls of the beaker) and then read the
ο
thermometer to the nearest 0.1 C.
• Record your results on the Report Sheet.
4- Convert your answers to questions 1, 2, and 3 into F and K.

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