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    LAB 5 - Plant Cell Physiology

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    دوار الشمس
    This document describes a lab experiment on diffusion and osmosis in plant cells. Students use atsuete seeds and boat of Moses plant leaves to demonstrate and observe diffusion of pigments and osmosis. They observe cells under a microscope at different magnifications as they are placed in water, salt solution, and water again to see the effects on cell turgor pressure and shape. The objective is to demonstrate and understand diffusion and osmosis processes essential for plant cell function.

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    LAB 5 - Plant Cell Physiology

    Uploaded by

    دوار الشمس
    15 views3 pages
    This document describes a lab experiment on diffusion and osmosis in plant cells. Students use atsuete seeds and boat of Moses plant leaves to demonstrate and observe diffusion of pigments and osmosis. They observe cells under a microscope at different magnifications as they are placed in water, salt solution, and water again to see the effects on cell turgor pressure and shape. The objective is to demonstrate and understand diffusion and osmosis processes essential for plant cell function.

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    This document describes a lab experiment on diffusion and osmosis in plant cells. Students use atsuete seeds and boat of Moses plant leaves to demonstrate and observe diffusion of pigments and osmosis. They observe cells under a microscope at different magnifications as they are placed in water, salt solution, and water again to see the effects on cell turgor pressure and shape. The objective is to demonstrate and understand diffusion and osmosis processes essential for plant cell function.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    15 views3 pages

    LAB 5 - Plant Cell Physiology

    Uploaded by

    دوار الشمس
    This document describes a lab experiment on diffusion and osmosis in plant cells. Students use atsuete seeds and boat of Moses plant leaves to demonstrate and observe diffusion of pigments and osmosis. They observe cells under a microscope at different magnifications as they are placed in water, salt solution, and water again to see the effects on cell turgor pressure and shape. The objective is to demonstrate and understand diffusion and osmosis processes essential for plant cell function.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Name: ___________________________________________________________________ Rating: _______________________

    ___________________________________________________________________
    ___________________________________________________________________
    ___________________________________________________________________
    ___________________________________________________________________ Date Performed: _____________
    Course/Section: ______________________________________________________________ Date Submitted: _____________

    Activity No. _____


    PLANT CELL PHYSIOLOGY
    Diffusion and Osmosis

    I. Introduction
    The cell is the basic unit of function and structure in all living things. Cell functioning is very important
    especially its ability to absorb and release materials/substances from and outside the environment. Diffusion is
    the process in which there is movement of a substance from an area of high concentration of that substance to an
    area of lower concentration and is the simplest means of material transport. On the other hand, Osmosis is a
    special type of diffusion wherein molecules diffuse through a semipermeable membrane from a place of higher
    concentration to a place of lower concentration until the concentration on both sides is equal. Both mechanisms
    are essential to facilitate transport of substances necessary for various life functions.

    II. Objective
    At the end of the activity, you should be able to demonstrate diffusion process using selected plant pigments
    and osmosis.

    III. Materials
    Atsuete seeds distilled water vegetable oil
    Transparent disposable cups water bath weighing scale

    fresh Tradescantia spatheca Sw (boat of Moses) glass slides & cover slips
    Dissecting needle & razor blade dropper, tissue paper
    Compound microscope water, 5% salt solution

    IV. Methods/Procedures
    Diffusion:
    1. Put 1 g of atsuete seeds into each of four cups. Label them with numbers 1-4.
    2. Pour onto the cups the following:
    a) C1 - put 10ml distilled water
    b) C2 - put 10ml of recently boiled distilled water
    c) C3 - put 10ml vegetable oil
    d) C4 - put 10ml of heated vegetable oil
    3. Leave the cups for 30 minutes. Shake the cups after and observe the colors in each tube. Compare the
    color intensities. Indicate the darkest by +++, then next ++, the lightest with +. Fill in the table.

    Osmosis
    4. Turgidity
    a) Get fresh leaf of T. spathecea. Cut off a thin and small section of the lower epidermis.
    b) Make a fresh mount of this specimen and observe under low power objective.
    c) Look for purple colored cells. Their cell sap has dissolved anthocyanin pigment.
    d) Swing to the high power objective and examine the cells and the cell wall. Draw the cells.
    5. Plasmolysis
    a) Add salt solution to the fresh mount at the side of the cover slip.
    b) Study the changes in the cell upon addition of the solution. Observe under low and high power
    objectives and make an illustration of the cells.
    6. Deplasmolysis
    a) Draw out the salt solution by putting a small portion of tissue paper at one side of the cover.
    b) Add a drop of pure water on the mount. Study the changes in the cell wall and draw.

    V. Date and Results


    Table 3. Color intensities of cups containing atsuete soaked in different substances.
    Substance Color Intensity with explanation/description

    C1

    C2
    C3

    C4

    Paste pictures of the set up results below:

    Cell observations: Identify Total Magnification and label your observations.

    LPO LPO LPO

    HPO HPO HPO

    Normal cell in water Plasmolyzed cell in salt solution Deplasmolyzed cell in water
    (Turgid) (flaccid) (turgid)

    VI. Post Lab Questions:


    1. Which plant pigment traveled farthest? Why?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    2. What other factors affect rate of diffusion? Explain.
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    3. In a turgid cell, what accounts for water movement into the proptoplasm?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    4. Explain osmotic flow when a living plant cell is placed in a strong salt solution.
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    5. What is incipient plasmolysis?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    6. Is plasmolysis reversible? Why?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    7. What are the membranes that envelope the vacuole of a plant cell?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    8. What is found between the cell wall and cell membrane in a plasmolyzed cell?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    9. In areas where weeds are predominant, salt solutions are usually used to destroy the weeds. Why is this
    so?
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________
    _________________________________________________________________________________________________________________________

    VII. References. (Write down all references/literature/citations used for this exercise.

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