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Transcription Worksheet

This document contains questions about transcription and related concepts like RNA, RNA polymerase, initiation and termination of transcription, splicing, genetic disorders related to transcription, drugs targeting transcription, DNA/RNA/tRNA/amino acid sequences, melting temperatures, promoters, and reverse transcription. It asks the learner to identify key elements of transcription like promoter regions, start sites, primer sequences for reverse transcription, and differences between pre-mRNA and mature mRNA transcripts.

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Gilbert
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25 views4 pages

Transcription Worksheet

This document contains questions about transcription and related concepts like RNA, RNA polymerase, initiation and termination of transcription, splicing, genetic disorders related to transcription, drugs targeting transcription, DNA/RNA/tRNA/amino acid sequences, melting temperatures, promoters, and reverse transcription. It asks the learner to identify key elements of transcription like promoter regions, start sites, primer sequences for reverse transcription, and differences between pre-mRNA and mature mRNA transcripts.

Uploaded by

Gilbert
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Cell and Molecular Biology

Transcription Worksheet

1. What is transcription and how does it relate to the central dogma of molecular biology?

2. What are the main types of RNA involved in transcription, and what are their function

3. What role does RNA polymerase play in transcription?

4. How does RNA polymerase know where to start transcribing a gene?

5. Outline the key steps of transcription, from initiation to termination.

6. How does termination of transcription differ between prokaryotes and eukaryotes?

7. Why is splicing necessary for many eukaryotic genes, and how is it accomplished?

8. Discuss one genetic disorders related to transcriptional abnormalities. How do these disorders

manifest at the molecular level?

9. Provide examples of drugs that target transcriptional processes for therapeutic purposes.

10. For the following sequences, fill in the DNA, the mRNA sequence, the tRNA anticodons, or the
amino acid sequences that have been left blank. If several sequences might work choose any one.

DNA ___

mRNA A U G A C U A G C U G G G G G U A U U A C U U U U A G

tRNA ___

AA ___

11. Below is a non-template (coding region) DNA strand sequence of Gene X.

3’-… CAA ACG GAG GAG CTT TAA CCT TAA…-5’

What is the mRNA sequence? Provide and label the 5’ to 3’ orientation of the transcript.
12. The base composition of a virus was found to be 11% A, 32% G, 18% U and 39% C. It this

a DNA or RNA virus? How can you tell? Is it single-stranded or double-stranded? How can you

tell?

13. Which of these DNA fragments will have a higher melting temperature? (circle one)

A) GCATTGACCGGAGGGACT B)GGATTTCAATTACTTAAT

CGTAACTGGCCTCCCTGA CCTAAAGTTAATGAATTA

14.Below is a portion of a bacterial chromosome that contains a gene. The promoter region and the +1
base pair are indicated, as well as the polarity of the two DNA strands.

a)Identify the i) promoter ii) transcription start site, iii) sequences downstream and upstream of the
start site

b. Write the first 6 nucleotides of the RNA molecule transcribed from this gene. Be sure to indicate
the 5’ and 3’ ends of the strand.

15.How does a pre-mRNA transcript differ from a mature mRNA transcript?

16.
Enzyme X

17. i)Name the process illustrated above. Where does it take place?

ii)What is enzyme X?

iii)Explain why the primer sequence is made up of oligo dTs (thymines nucleotides)?

End.

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