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Proposal

This document outlines a study that will test the toxicity of Vitex parviflora (Molave) leaves extract on Danio rerio (zebrafish). Zebrafish are increasingly used as a toxicological model for rapid testing due to their robustness, high genetic homology to mammals, and visible phenotypes. The purpose of this study is to measure the toxicity of Vitex parviflora extract by analyzing its effects on zebrafish at different concentrations to determine a safe or toxic dosage level.

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Janisa Sacaria
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47 views2 pages

Proposal

This document outlines a study that will test the toxicity of Vitex parviflora (Molave) leaves extract on Danio rerio (zebrafish). Zebrafish are increasingly used as a toxicological model for rapid testing due to their robustness, high genetic homology to mammals, and visible phenotypes. The purpose of this study is to measure the toxicity of Vitex parviflora extract by analyzing its effects on zebrafish at different concentrations to determine a safe or toxic dosage level.

Uploaded by

Janisa Sacaria
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Toxicology testing on Danio rerio (zebrafish) using Vitex

parviflora (Molave) leaves extract

Introduction

Toxicology is the study of the adverse effects of chemical,


physical, or biological agents on living organisms and the ecosystem.
Toxicology testing is also known as safety assessment, or toxicity testing,
is the process of determining the degree to which a substance of interest
negatively impacts the normal biological functions of an organism, given a
certain exposure duration, route of exposure, and substance concentration

The hazard identification and dose-response assessment steps are


primarily based on a number of different tests where animals are exposed
to the chemical or test substance. A toxicity test, by extension, is designed
to generate data concerning the effects of a substance on human or animal
health, or the environment. Many toxicity tests examine specific types of
adverse effects, known as endpoints.

I. Background of the Study

Embryonic and larval Danio rerio (zebrafish) is increasingly used


as a toxicological model to conduct rapid tests and developmental toxicity
assays; the zebrafish features high genetic homology to mammals, robust,
and phenotypes.

In the early 1970s, the zebrafish was used as a model system, when
it was selected to develop the first vertebrate assay enabling forward
genetic screening. During the following 30 years, zebrafish were almost
always used to study organ developments. This resulted in the personation
of an exceptionally large number of genes involved in vertebrate
pathways, which contributed to the establishment of zebrafish as a
relevant model for human disease and pharmaceutical research. For
pharmacology an attractive feature of zebrafish assays is the prospective to
use them in medium-to-high-throughput screening mode, because the
zebrafish is a small and robust fish that it is easy to maintain thanks to
their high fertility
II. Statement of the Problem

Excessive absorption or use of the Vitex parviflora (Molave) could


cause virulent effects to organisms.

III. Significance of the Study

The importance of toxicity testing is to check the effect of the test


substances on laboratory animals and its direct toxic effect on humans and
secondly, the exposure of laboratory animals to high doses in order to
evaluate its possible hazard on humans that are exposed to much lower
dose.

Vitex parviflora (Molave) is known for being a remedy for


poisons, diarrhea, and kidney stones and in this study we are to examine at
what concentration of Vitex parviflora (Molave) is it safe or deadly to our
animal model, Danio rerio (Zebrafish).

IV. Objectives of the Study

The purpose of this study is to measure the toxicity in a Vitex


parviflora (Molave) by analysing the results that the exposure produces on
Danio rerio (Zebrafish).

Literature Cited
- Zebrafish as screening model for detecting toxicity and drugs
efficacy by Maria Virginia Caballero, Feb 2018
- Toxicity Studies of Plant Extracts on Insects and Fish by
Mohd. Aspollah, Shozo and Abd. Rahman, 1992
- Principles of Toxicology Testing 2nd Edition by Dr Frank A.
Barile, 2013
- Environmental Toxicology by Chris Theodorakis, in
Encyclopedia of Toxicology 2nd Edition, 2005
- Toxicology Testing and Evaluation by E. Zeiger, in
Comprehensive Toxicology, 2010

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