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    MT1 - BIOCHEM - LAB - MT - EXP7 - Test Summary PDF

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    12 ABMJonica Iris Bolina
    This document summarizes several experiments conducted to analyze coconut oil and glycerol. It describes tests for solubility, pH, formation of translucent spots, emulsification, double bonds, unsaturation, glycerol esters, and primary/secondary alcohols. For each test, it lists the purpose, materials used, and potential positive and negative results observed.

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    MT1 - BIOCHEM - LAB - MT - EXP7 - Test Summary PDF

    Uploaded by

    12 ABMJonica Iris Bolina
    18 views2 pages
    This document summarizes several experiments conducted to analyze coconut oil and glycerol. It describes tests for solubility, pH, formation of translucent spots, emulsification, double bonds, unsaturation, glycerol esters, and primary/secondary alcohols. For each test, it lists the purpose, materials used, and potential positive and negative results observed.

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    MT1_BIOCHEM_LAB_MT_EXP7_Test%20Summary.pdf

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    This document summarizes several experiments conducted to analyze coconut oil and glycerol. It describes tests for solubility, pH, formation of translucent spots, emulsification, double bonds, unsaturation, glycerol esters, and primary/secondary alcohols. For each test, it lists the purpose, materials used, and potential positive and negative results observed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    18 views2 pages

    MT1 - BIOCHEM - LAB - MT - EXP7 - Test Summary PDF

    Uploaded by

    12 ABMJonica Iris Bolina
    This document summarizes several experiments conducted to analyze coconut oil and glycerol. It describes tests for solubility, pH, formation of translucent spots, emulsification, double bonds, unsaturation, glycerol esters, and primary/secondary alcohols. For each test, it lists the purpose, materials used, and potential positive and negative results observed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    EXPERIMENT 7

    Test Purpose Materials Used Positive Result Negative Result Picture

    Test for Reagents Formation of one layer Formation of two layers


    Solubility in the sol’n in the sol’n
    - water
    - diluted HCl
    - diluted NaOH - coconut oil + water (H2O)
    - hot, cold alcohol - coconut oil + dil.
    - determines how much - coconut oil + ether (C4H10O)
    Solubility Test - ether hydrochloric acid (HCl)
    solute is or can be - coconut oil + chloroform
    (for coconut oil) - CHCl3 - coconut oil + dil. sodium
    dissolved in a solvent (CHCl3)
    - CCl4 hydroxide (NaOH)
    at equilibrium - coconut oil + carbon
    - coconut oil + cold alcohol
    - nonpolar attracts tetrachloride (CCl4)
    Reactants (C2H5OH)
    nonpolar, polar attracts - glycerol + water (H2O)
    - coconut oil + hot alcohol
    polar - glycerol + alcohol (C2H5OH)
    - coconut oil (nonpolar) (C2H5OH)
    - glycerol (polar) - glycerol + ether (C4H10O)

    Test for Reagents Blue-Black coloration No change in coloration


    pH of the sol’n with Congo Red of the sol’n with Congo Red

    - congo red
    (C32H22N6Na2O6S) - Coconut oil
    - litmus paper - Rancid oil
    (red)

    - fresh oil is neutral but Reactants


    Reaction towards Blue coloration No change in coloration
    when exposed to an
    Indicators of the Red Litmus Paper of the Red Litmus Paper
    open environment, it
    undergoes oxidation
    called rancidity - Coconut oil
    - (-OH) in oils will turn - Rancid oil
    to (COOH) - fresh coconut oil
    - rancid oi Red coloration No change in coloration
    of the Blue Litmus Paper of the Blue Litmus Paper

    - rancid oil - coconut oil

    Test for Presence of Material Translucent Spot Formation No Translucent Spot


    Translucent Spot in the paper Formation in the paper
    - piece of paper

    Reactants
    Formation of - essential oils
    Translucent Spot (coconut oil) wouldn’t
    leave translucent spots - coconut oil
    unless they’re actual
    oils - coconut oil

    Test for Reagents Piercing Acrid/Burnt


    Acrolein Formation Fat/Burnt Yeast odor

    - process: dehydration - KHSO4


    - acrolein is the
    Acrolein Test
    simplest unsaturated
    - coconut oil
    aldehyde caused by
    Reactants - glycerol (C3H8O3)
    dehydration of glycerol
    - KHSO4 as
    dehydrating agent - coconut oil
    - glycerol

    Test for Reagents White ppt No ppt


    Miscibility in clear sol’n in clear sol’n
    - water
    - water and soap sol’n
    - water and albumin - coconut oil + water and soap
    - water and sodium sol’n (permanent emulsion) add another picture for reference
    Emulsification - combining 2 liquids carbonate - coconut oil + water and kay wala nakoy lain sources :((
    that do not usually mix albumin (temporary emulsion)
    - coconut oil + water (H2O)
    together with the use of Reactants - coconut oil + water and
    an emulsifying agent sodium carbonate
    (H2O + Na2CO3)
    - coconut oil (temporary emulsion)

    Test for Reagents Brown coloration No coloration


    Double Bonds (unsaturated) in clear sol’n (saturated) in clear sol’n
    - Hubl’s I2 Solution
    a.) HgCl2 in 95%
    C2H5OH
    b.) I2 in 95%
    Test for C2H5OH
    - fats that contain - stearic acid (lightest)
    Unsaturation
    unsaturated fatty acids - oleic acid
    Reactants
    will have a positive - coconut oil
    result - linseed oil (darkest)
    - oleic acid
    - stearic acid
    - coconut oil
    - linseed oil

    Test for the presence Reagents Green flame on the tip Red-Orange flame on the tip
    of Glycerol Esters of the Nichrome Wire of the Nichrome Wire
    - Powdered Borax
    a.) Na2B4O7(H2O)10
    Flame Test
    (for glycerol) - A green flame is Reactants
    produced if glycerol is - glycerol (C3H8O3)
    fused with boric acid
    - glycerol

    Test for Reagents Blue coloration


    1o and 2o Alcohols of the sol’n
    - conc. HNO3
    - K2CrO4
    Nitro-Chromic - detection of primary Reactants
    Acid Test and secondary alcohol
    - the hydroxyl group - glycerol (C3H8O3)
    (-OH) of glycerol reacts
    with the reagents - glycerol

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