B I O -V I S I ON © S O F T WA R E

USER & SERVICE MANUAL - V 18.0 1

I N F I N I T Y CX5 E D G E
VILBER is pleased to announce the release of a new Western
Blot immunodetection process to enhance our current Western

Q U A N T U M CX5 E D G E
Blot and fluorescent documentation product portfolio, E-Box
Purity.

This Sales Manual is intended to provide a thorough collection of

B I O -P R I N T CX4 E D G E
product information for sales and training support.

E -B O X CX 5 E D G E

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 I NFINI TY © CX5 E DGE
Q UANTUM © CX5 E DGE
B I O -P RI NT © CX4 E DGE
E-B OX © CX5 E DGE
USER & SERVICE MANUAL – SOFTWARE V18.01
Thank you
Dear Customer,

On behalf of Vilber Lourmat, we would like to thank you for choosing our imaging system.

In order to learn the capabilities of your imaging system, we kindly ask you to read this manual. This manual details
how to install and to operate the hardware and the software components.

Vilber Lourmat is dedicated to your satisfaction and we will be pleased to answer any question you may have. We
are also very receptive to your suggestions. Many of the new features and enhancements in this system are a direct
result of conversations with our customers. Please do not hesitate to contact us to let us know what you would like
to see in the next version of this system.

You can contact us at the following addresses:

 Email: [email protected]

 Worldwide: Vilber Lourmat SAS

BP31 - ZAC de Lamirault- Collegien - F-77601 Marne-la-Vallée Cedex 3 - France
T.: +33 (0) 1 60 06 07 71

 Germany only: Vilber Lourmat Deutschland GmbH

Wielandstrasse 2 - D-88436 Eberhardzell - Deutschland
T.: +49 (0) 7355 931 380

 China only: Vilber China

Room 127 Building A - N° 111 Yuquangying - Fengtai District – Beijing - China
T.: + 86 1361 1131 545

Do not hesitate to visit our website at www.vilber.com

Rev. D – June-2018

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Table of contents
Notice................................................................................................................................................................ 10
Trademarks ....................................................................................................................................................... 10
Introduction ...................................................................................................................................................... 11
Using this manual ........................................................................................................................................................ 11
Prerequisites ............................................................................................................................................................... 11
Warranty ..................................................................................................................................................................... 11
Safety & regulatory compliance .......................................................................................................................... 13
Introduction ................................................................................................................................................................ 13
Safety notices .............................................................................................................................................................. 13
General precaution ..................................................................................................................................................... 14
Emergency procedure ................................................................................................................................................. 16
Important UV safety information ............................................................................................................................... 17
Important electrical power safety information .......................................................................................................... 19
Instrument serial number and manufacturing information ....................................................................................... 21
CE conformity .............................................................................................................................................................. 21
International standards............................................................................................................................................... 21
Recycling information ........................................................................................................................................ 22
Decontamination ........................................................................................................................................................ 22
Recycling of UV lamps ................................................................................................................................................. 22
Disposal of electrical components .............................................................................................................................. 22
E-BOX CX5 EDGE ................................................................................................................................................ 23
About the E-BOX CX5 EDGE imaging system .............................................................................................................. 23
E-BOX CX5 EDGE system installation ................................................................................................................... 23
Unpacking the system ................................................................................................................................................. 23
Protect the environment! ........................................................................................................................................... 24
Installing the hardware ............................................................................................................................................... 26
Environmental conditions ....................................................................................................................................... 26
Imager back side ..................................................................................................................................................... 27
E-BOX CX5 EDGE hardware operating instruction ................................................................................................ 28
Warning while using the darkroom ............................................................................................................................ 28
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 Imager rear side ...................................................................................................................................................... 29
Imager front side – Door open ................................................................................................................................ 29
Optional PadBox and Pad system ........................................................................................................................... 30
Filter wheel ............................................................................................................................................................. 32
E-BOX CX5 EDGE software interface operation .................................................................................................... 33
Introduction ................................................................................................................................................................ 33
Start the E-BOX CX5 EDGE system & software ........................................................................................................... 34
Capture an image ........................................................................................................................................................ 35
Fluorescence image acquisition .............................................................................................................................. 35
Image capture workflow ......................................................................................................................................... 35
Choose the application type ................................................................................................................................... 36
Acquire the image ................................................................................................................................................... 37
Save or print the image ........................................................................................................................................... 42
Inverse ................................................................................................................................................................. 43
Adjust the image display ..................................................................................................................................... 44
The Application protocol .................................................................................................................................... 46
What is an Application protocol?................................................................................................................................ 46
How to access the application protocol? .................................................................................................................... 46
Advanced exposure – Exposure predefined set-up ................................................................................................ 47
Preview – Live preview pre-defined parameters .................................................................................................... 51
Display – Image display pre-defined parameters ................................................................................................... 52
Printing – Image printing pre-defined parameters ................................................................................................. 54
General – Image file pre-defined parameters ........................................................................................................ 55
The Apps studio....................................................................................................................................................... 56
The System settings ........................................................................................................................................... 58
The general system settings........................................................................................................................................ 59
Language selection...................................................................................................................................................... 59
Mail to ......................................................................................................................................................................... 61
Windows parameters .................................................................................................................................................. 62
Lock / unlock the E-Box system .............................................................................................................................. 62
Add a printer ........................................................................................................................................................... 62
Set the network parameters ................................................................................................................................... 63
Connect to the E-Box from your computer .......................................................................................................... 65
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 Connect using your internet browser ......................................................................................................................... 65
Connect using the Bio-Vision software ....................................................................................................................... 65
How to map a network drive .............................................................................................................................. 69
Update the E-Box firmware ................................................................................................................................ 73
Export system .................................................................................................................................................... 74
Run TeamViewer for premium service support ................................................................................................... 74
INFINITY CX5 ...................................................................................................................................................... 75
About the INFINITY CX5 EDGE imaging system........................................................................................................... 75
INFINITY CX5 EDGE system installation ............................................................................................................... 75
Unpacking the system ................................................................................................................................................. 75
Installing the hardware ............................................................................................................................................... 77
Environmental conditions ....................................................................................................................................... 77
Imager back side ..................................................................................................................................................... 78
INFINITY CX5 EDGE hardware operating instruction ............................................................................................ 79
Warning while using the darkroom ............................................................................................................................ 79
Imager front side – Door open ................................................................................................................................ 79
Optional PadBox and Pad system ........................................................................................................................... 80
Filter wheel ............................................................................................................................................................. 81
QUANTUM CX5 .................................................................................................................................................. 82
About the QUANTUM CX5 imaging system ................................................................................................................ 82
QUANTUM CX5 system installation .................................................................................................................... 82
Unpacking the system ................................................................................................................................................. 82
Installing the hardware ............................................................................................................................................... 84
Environmental conditions ....................................................................................................................................... 84
Imager back side ..................................................................................................................................................... 85
QUANTUM CX5 hardware operating instruction ................................................................................................. 85
Warning while using the darkroom ............................................................................................................................ 85
Imager front side – Door open ................................................................................................................................ 86
Optional PadBox and Pad system ........................................................................................................................... 87
Filter wheel ............................................................................................................................................................. 88
BIO-PRINT CX4 ................................................................................................................................................... 89
About the BIO-PRINT CX4 EDGE imaging system ........................................................................................................ 89

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BIO-PRINT CX4 EDGE system installation ............................................................................................................ 89
Unpacking the system ................................................................................................................................................. 89
Installing the hardware ............................................................................................................................................... 91
Environmental conditions ....................................................................................................................................... 91
Imager back side ..................................................................................................................................................... 92
BIO-PRINT CX4 EDGE hardware operating instruction ......................................................................................... 92
Warning while using the darkroom ............................................................................................................................ 92
Imager front side – Door open ................................................................................................................................ 93
BIO-VISION software – PC version ...................................................................................................................... 94
BIO-VISION software installation ........................................................................................................................ 94
Minimum computer configuration ............................................................................................................................. 94
How to enable the XHCI Bios option ................................................................................................................... 95
Prerequisite ................................................................................................................................................................. 96
Bio-Vision software installation .................................................................................................................................. 96
Trouble shooting – Windows device manager ......................................................................................................... 102
Configure your PC to improve the system performance .......................................................................................... 104
Bios settings – Disable the CPU EIST function....................................................................................................... 104
USB Advanced Power options ............................................................................................................................... 104
USB Hub Advanced Power options ....................................................................................................................... 106
BIO-VISION - Image Capture Operation ........................................................................................................... 107
Introduction .............................................................................................................................................................. 107
Start the imaging system & software ....................................................................................................................... 108
Capture an image ...................................................................................................................................................... 109
Fluorescence image acquisition ............................................................................................................................ 109
Image capture workflow ....................................................................................................................................... 109
Choose the application type ................................................................................................................................. 110
Acquire the image ................................................................................................................................................. 111
Adjust the zoom and focus ............................................................................................................................... 112
Display the saturation ....................................................................................................................................... 113
Display the 3D image ........................................................................................................................................ 114
Display the inversed image ............................................................................................................................... 114
Save or print the image ......................................................................................................................................... 116

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BIO-VISION - The Display Menu ...................................................................................................................... 119
Adjust the image display range ......................................................................................................................... 119
Display the saturation ....................................................................................................................................... 120
Display the 3D image ........................................................................................................................................ 121
Display the inversed image ............................................................................................................................... 122
Autofit or zoom in / out on the image .............................................................................................................. 122
Access the Good Laboratory Practice (GLP) file ................................................................................................ 123
Display the image using pseudo colors ............................................................................................................. 123
BIO-VISION - The Edit Menu............................................................................................................................ 125
Text ........................................................................................................................................................................... 125
Crop the image .......................................................................................................................................................... 130
Rotation..................................................................................................................................................................... 131
Background subtraction ............................................................................................................................................ 133
Flat field .................................................................................................................................................................... 134
Artifact correction ..................................................................................................................................................... 135
Multiplexing .............................................................................................................................................................. 135
BIO-VISION - The Application Protocol ............................................................................................................ 139
What is an Application protocol?.............................................................................................................................. 139
How to access the application protocol? .................................................................................................................. 139
Advanced exposure – Exposure predefined set-up .............................................................................................. 140
Preview – Live preview pre-defined parameters .................................................................................................. 144
Display – Image display pre-defined parameters ................................................................................................. 145
Printing – Image printing pre-defined parameters ............................................................................................... 147
General – Image file pre-defined parameters ...................................................................................................... 148
BIO-VISION - The System Settings ................................................................................................................... 150
The general system settings.................................................................................................................................. 150
Language selection................................................................................................................................................ 151
CFR21 mode activation ......................................................................................................................................... 152
Export system........................................................................................................................................................ 152
Run TeamViewer for premium service support .................................................................................................... 153
BIO-VISION - The Analysis Menu ..................................................................................................................... 155
Access the analysis software..................................................................................................................................... 155

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Analysis software interface ....................................................................................................................................... 155
Print ................................................................................................................................................................... 157
Black / White bands .......................................................................................................................................... 157
Copy to clipboard .............................................................................................................................................. 158
Display the molecular weight............................................................................................................................ 159
Image display parameter .................................................................................................................................. 159
Molecular weight analysis module ........................................................................................................................... 165
Detect – Edit lane .................................................................................................................................................. 165
Detect – Edit bands ............................................................................................................................................... 168
Detect – Add marker values .................................................................................................................................. 171
Molecular weight results ...................................................................................................................................... 175
Return to the image acquisition module .............................................................................................................. 176
Quantification analysis module ................................................................................................................................ 177
Detect – Lane definition ........................................................................................................................................ 177
Background subtraction ........................................................................................................................................ 180
Spot separation ..................................................................................................................................................... 184
Spot quantification................................................................................................................................................ 186
Quantity of reference............................................................................................................................................ 187
Return to the image acquisition module .............................................................................................................. 190
Colony counting analysis module ............................................................................................................................. 192
Objectives and output........................................................................................................................................... 192
Automatic counting............................................................................................................................................... 193
Detection........................................................................................................................................................... 193
Return to the Capt acquisition module ............................................................................................................. 194
Manual counting ................................................................................................................................................... 195
Return to the Capt acquisition module ............................................................................................................. 196
Distance calculation module ..................................................................................................................................... 197
Objectives and output........................................................................................................................................... 197
1-Detect................................................................................................................................................................. 197
A – Edit lane ...................................................................................................................................................... 197
B – Edit band ..................................................................................................................................................... 200
C – Distance ....................................................................................................................................................... 204
2- Analyse – Distance ............................................................................................................................................ 207
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A – Distance results ........................................................................................................................................... 207
Return to the Capt acquisition module ............................................................................................................. 208

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Notice
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical,
including photocopy, recording, or any information storage or retrieval system, without permission in writing from
Vilber Lourmat.

Vilber Lourmat reserves the right to modify its products and services at any time without prior notice.

The contents of this user guide are subject to change without notice. This user guide has been prepared with utmost
care. Vilber Lourmat assumes no liability for errors or omissions, or for any damage resulting from the application or
use of this information. If you have any questions of find errors, omissions, etc., please contact us.

This instrument is suitable for research use only. Some techniques or reagents for generating and / or detecting light
in biological samples are patented and may require licenses from third parties. Users have to independently
determine for themselves whether their activities infringe any valid patent. Vilber Lourmat cannot be held
responsible for patent infringement resulting by the inappropriate user activities.

All goods and services are sold subject to the terms and conditions of sale of Vilber Lourmat SAS. A copy of these
terms and conditions is available on request.

Copyright © 2004-2018 by Vilber Lourmat SAS. All rights reserved.

Trademarks
Windows, Windows 10, Windows 8, Windows 7, Windows XP, Windows Vista, Excel, Microsoft, and Power Point are
trademarks of Microsoft Corporation.
Intel Pentium, Intel Core are trademarks of Intel Corporation.
FireWire, Mac, and Mac OS X are trademarks of Apple Inc.
Coomassie is a trademark of BASF Aktiengesellschaft.
Amersham, Cy and ECL Plex are trademarks of General Electric Company.
Alexa Fluor, Coomassie Fluor, Q-dot, SYBR Safe, SYBR Green, SYBR Gold, and SYPRO are trademarks of Life
Technologies Corporation.
Gel-Green and Gel-Red are trademarks of Biotium, Inc.
Dy-Light and Krypton are trademarks of Thermo Fisher Scientific Inc.

Other brands or product names are trademarks of their respective holders.

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Introduction
Using this manual
This manual contains software and hardware instructions for the following imaging systems:
• Infinity CX5 Edge
• Quantum CX5 Edge
• E-Box CX5 Edge
• Bio-Print CX4 Edge
Please refer to the specific pages of your instrument.

This manual is part of the product. Please read this manual in full before using your imaging system
• Store this manual at an easily accessible location
• The device may only be transferred with the operating manual
• If the operation manual is lost, replace it immediately. Please contact us for further details.

Prerequisites
The User Guide provide you with the instructions needed to operate, and maintain your imaging system in a safe
way.

In order to operate your imaging system in the appropriate way, the following prerequisites must be fulfilled:
• You have read and understood the safety instructions in this User Guide, particularly in regards of UV danger.
• You should be qualified to use general laboratory equipment and to handle biological materials.
• You should use the instrument only for the intended purpose of gel and blot image acquisition in research
laboratories.
• You accept the terms and conditions of the end user license agreement
• The instrument is connected to a grounded power source and to a circuit breaker.
• The instrument is installed by a Vilber Lourmat representatives.
• This instrument is suitable for research use only and shall not be used in clinical procedures, or for diagnostic
purposes.
• Some techniques or reagents for generating and / or detecting light in biological samples are patented and may
require licenses from third parties. Users should independently determine for themselves whether their activities
infringe any valid patent before operating your imaging system.
• Some configurations involve UV illumination. This instrument should be used only by trained personnel who know
the health risks associated with the UV radiation normally associated with this instrument. Users should be trained
on the appropriate personal protection gear for working with UV light to minimize UV exposure.

Warranty
Our imaging system is warranted against faulty construction or defective material for a period of two years from the
Vilber Lourmat invoicing date. If any defect occurs in the instrument during this warranty period, Vilber Lourmat will
repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically
excluded:
• Defects caused by improper operation, incorrect use or bad maintenance
• Repair or modification done by anyone other than Vilber Lourmat or the company’s authorized agent
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• Use of spare parts supplied by anyone other than Vilber Lourmat.
• Damage caused by accident, misuse or disaster
• Corrosion caused by improper solvents or samples

This instrument should not be modified or altered in any way. Modification or alteration of this instrument will:
1. Void the manufacturer’s warranty.
2. Void the conformity certifications.
3. Create a potential safety hazard.

The light sources (LED panel, UV tubes …), the filters, the power supplies, the batteries, and the consumables are not
covered by our warranty. The use of consumable products or non-original spare parts not recommended by our
service department is at the user's own risk and therefore automatically invalidates the warranty.

We reserve the right to decide where the faulty goods will be repaired (in our workshop or elsewhere), and whether
or not the faulty part is to be replaced; all other freight charges incurred being at the cost of the purchaser. Returned
goods will not be accepted for repair unless previous written authorization is obtained from our service department.
A request for authorization must be accompanied by an itemized list of products, model numbers and the
corresponding invoice numbers under which they were originally shipped. All returned goods should have a
certificate of decontamination. The Buyer must bear all costs and risks incurred during the transportation of the
goods from their collection at Vilber Lourmat factory. In the case Vilber Lourmat incorporates some devices or
equipment from another supplier in the manufacture of its products, the extent and the duration of the warranty
will be those conceded by the suppliers or sellers.

Vilber Lourmat cannot be held responsible for any loss, bodily injury or material accident incurred by any failure of
this supply, whatever the origin of this failure may be. Vilber Lourmat is not responsible for any injury or damage
caused by use of this instrument for purposes other than those for which it is intended, or by modifications of the
instrument not performed by Vilber Lourmat. The responsibility of Manufacturer is strictly limited to its staff and to
its own supplies. In the case of dispute, only the commercial court of Meaux (FRANCE) shall be competent, even in
third party claims proceedings or when there are several co-defendants.

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Safety & regulatory compliance
Introduction

PLEASE READ CARFULLY THESE INSTRUCTIONS BEFORE OPERATING THE INSTRUMENT.

Your imaging system is powered by mains voltage, may be equipped with a UV light source, and is used to capture
images of samples that may be hazardous. Before installing, operating or maintaining the instrument, you must be
aware of the hazards described in the user documentation. Follow the instructions provided to avoid personal injury
or damage to the instrument.

Safety notices
This user documentation contains safety notices (WARNING and UV WARNING) concerning the safe use of the
product. See definitions below.

WARNING
Whenever you find this pictograph, be sure to refer to this Manual.

WARNING indicates an imminently hazardous situation which, if not avoided, could result in
death or serious injury. It is important not to proceed until all stated conditions are met and
clearly understood.

UV WARNING
Whenever you find this pictograph, be sure to refer to this Manual.

UV WARNING indicates an imminently hazardous UV radiation situation which, if not avoided,

could result in serious injury. It is important not to proceed until all stated conditions are met
and clearly understood.

NOTICE
NOTICE indicates instructions that must be followed to avoid damage to the instrument or
other equipment or to ensure the instrument is properly working.

13
General precaution

WARNING
Do not operate your imaging system in any other way than described in the User Manual.

WARNING
Use of this instrument in other ways than those specified in the user documentation may
result in physical damage because of exposure to irradiated light or electric shock or injury
from touching an operating part.

WARNING
Do not use your imaging system in dangerous atmosphere or with dangerous materials for
which your imaging system has not been designed for.

WARNING
Do not use the instrument if smoke, atypical noises or odors can be perceived, or if the
instrument becomes unusually hot as this may result in fire or electric shock. Stop using the
instrument immediately, turn off the power switch, and unplug the instrument from the
power outlet. Contact Vilber Lourmat representative to request repair.

WARNING
To prevent fire or shock hazard, do no expose the unit to rain or moisture.

Do not pour liquids directly on or inside the instrument.

Switch off all the lights immediately after use.

Clean the transilluminator platen after use.

14
WARNING
Ensure that all the ventilation-opening systems are not obstructed. The obstruction of the air
admission grids may affect the performance of the system and cause operational failure. To
ensure adequate cooling ensure there is at least 40 cm of free space in front of the instrument
and at least 10 cm on all other sides to walls or other equipment.

WARNING
The use of accessories not supplied by Vilber Lourmat can damage the system or create safety
hazard.

WARNING
When using hazardous chemicals, take all suitable protective measures, such as wearing
protective glasses and gloves resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of the system.

WARNING
Do not use the instrument within or near a sink, or in humid (above 70% RH) or dusty
environments. This can result in fire or electric shock.

WARNING
As the equipment is heavy, it cannot be carried by one person. Two service person are
necessary when moving or relocating safely the main body of the equipment. If the
equipment drops, it may be broken, or you may get seriously injured if it drops on your foot.

WARNING
Do not touch the light source in the instrument. The light source may be hot.

WARNING

• Do not place objects on top of the instrument.

• Use the handle to open or close the instrument door.

15
 • Handle sample trays with care to avoid dropping them.

• Do not leave samples in the instrument after exposure. If left, these may degrade and cause
damage to the instrument.

• Do not disable the interlock of the equipment as UV light can be emitted if the door is
opened.

• Do not open the instrument door during exposure. This will stop the exposure and image
data will be corrupted.

• Do not use abrasive cleaning materials for cleaning. This may cause damage to the
instrument.

• Do not use excessive amounts of liquids for cleaning

Emergency procedure

WARNING

In case of emergency:
• Turn off the power by pushing the Power switch to the O position on the top rear side of the
instrument.
• Disconnect the power cord from the AC voltage outlet.

Access to power switch and power cord with plug:

For your security, do not block the access to the power switch and to the power cord. The
power switch must always be easy to access and the power cord must always be easy to
disconnect.

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Important UV safety information

UV WARNING
In any UV WARNING situation, the operator should wear appropriate safety glasses or a
protective mask and gloves. UV radiation can be dangerous for unprotected eyes and skin;
therefore, we recommend the user to wear UV protective goggles (LP-70) or face-shield (MP-
80 or MP-800). Protect all skin surfaces (including the neck, ears, and hands).

Use of the UV transilluminator acrylic protective screen does not guarantee the user
protection from UV radiation. The use of protective goggles, mask, and/or gloves is strongly
recommended

This instrument should be used only by trained personnel who know the health risks
associated with the UV radiation normally associated with this instrument. Users should be
trained on the appropriate personal protection gear for working with UV light to minimize UV
exposure.

In its lowered position, the UV-Pad or UV transilluminator’s acrylic shield provides UV

protection. However, it does not provide complete protection to the user. In its raised
position, the acrylic shield does not protect others who are standing in the area around the
imager.

UV WARNING
Do not touch the UV unit after UV exposure. There is a risk of skin burn.

UV WARNING
The instrument is equipped with a safety interlock. If the interlock is out of order or has been
tampered with, UV and visible light may be emitted, which may cause skin burn and impair
vision.
Do not open the instrument door during imaging operation.

WARNING: The non-respect of these instructions can cause very serious burns to the user when the instrument is in
use.
WARNING: Ultra Violet light is dangerous for unprotected eyes and skin, therefore the user must wear UV
protective goggles (Ref. LP70) or a face-shield (Ref.MP-80 or MP-800)
SAVE THESE INSTRUCTIONS
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 AVISO: Durante el uso del instrumento, no seguir las instrucciones puede causar quemaduras graves al usuario.
PRECAUCION: La radiación ultravioleta puede ser peligrosa para los ojos y la piel expuestos sin protección. Para
protegerse, es impérativo usar gafas o una máscara.
CONSERVAR CUIDADOSAMENTE ESTAS INSTRUCCIONES.
AVERTISSEMENT: Lors de l’utilisation de cet appareil, le non-respect des instructions peut provoquer de graves
brûlures à l’utilisateur.

ATTENTION : Le rayonnement ultraviolet est dangereux pour les yeux et la peau exposée sans protection, il est
impératif de porter des lunettes (Réf. LP70) ou un masque de protection (Réf. MP-80 ou MP-800).

CONSERVER SOIGNEUSEMENT CES INSTRUCTIONS

AVVERTIMENTO : Durante l'utilizzo di questo apparecchio, il non rispetto delle istruzioni puo provocare
bruciature gravi all'utilizzatore.

ATTENZIONE : I raggi Ultra Violetti essendo pericolosi per gli occhi e la pelle esposti senza protezione, è
obbligatorio portare gli occhiali (Ref. LP 70) oppure una maschera di protezione (Ref. MP-80 o MP-800).

CONSERVARE CON CURA QUESTE ISTRUZIONI.

WARNUNG: während der Benutzung dieses Gerätes kann die Missachtung der Anleitung schwere Verbrennungen
an Personen hervorrufen.
ACHTUNG: Ultraviolettes Licht ist gefährlich für ungeschützte Augen und die Haut. Der Benutzer ist daher
angehalten, eine UV-Schutzbrille (Art. LP-70) oder ein Schutzschild (Art. MP-80 oder MP-800) zu tragen.
BEWAHREN SIE DIESE ANLEITUNG SORGFÄLTIG AUF

警告：使用此组件时，若不遵从使用指导将导致严重烧伤！

警告：紫外线对眼和皮肤有害，操作者必须佩戴紫外防护眼镜（货号：LP70）或面罩（货号 MP-80 或 MP-

800)

请保存使用指导！

警告：機器の使用に際して、説明書の指示に従わない場合、使用者が重大な熱傷を
こうむる場合があります。
警告：紫外線は裸眼や保護されていない皮膚にとって危険です。使用者はUV保護眼鏡 （Ref. LP70）または
フェースシールド（Ref. MP-80またはMP-800）を装着する必要が あります。
将来のためにこれらの説明書を保管しておいてください

18
Important electrical power safety information

WARNING
Electrical requirements are located on the imager label on the rear panel of the imager.
Please ensure these electrical requirements are compatible with your AC voltage outlet.

Connect the imaging system to an appropriate AC voltage outlet that is properly grounded
and protected by a circuit breaker.

Connect the imager power cord to the rear left side panel of the system and plug into a
grounded power outlet. Connecting to ground constitutes an obligatory protection.

WARNING
Power down the system and disconnect the AC main from the unit before performing any
disassembly or repair the instrument.

Before plugging or unplugging the camera cables, ensure the system is off and disconnect
your imaging system from the AC voltage outlet.

The system must be unplugged from the AC voltage outlet if it is not intended to use it before
a long time.

WARNING
Do not damage the power supply cord by bending, twisting, heating or allowing them to
become pinned under the equipment. Using damaged power cords could result in fire or
electric shock. If the power supply cords are damaged, contact us for replacements.

Never pull the cable itself. Disconnect the cable only by grasping the plug.

WARNING
Do not block access to the power switch and power cord. The power switch must always be
easy to access. The power cord with plug must always be easy to disconnect.

19
WARNING
The imaging system has serviceable fuses which are located on the rear panel and are a part
of the power entry module. See the Maintenance chapter of this guide for information about
replacing the fuses.

WARNING
All the equipment connected to this instrument shall be certified according to IEC 60950
standard.

WARNING
In case of thunder, do not touch the power supply plug, as this can result in electric shock.

WARNING
Turn off the power switch before cleaning the inside of the instrument.

WARNING
Do not turn off power during image acquisition as this can cause loss of data and damage the
instrument. Only turn off power in an emergency situation.

WARNING - Electrical shock hazard.

All instrument repairs or modifications should be performed by service personnel authorized
by Vilber Lourmat. Do not open any covers or replace parts unless specifically stated in the
user documentation.

20
Instrument serial number and manufacturing information
The instrument serial number is found on the label located on the back of the instrument. The two first digit refer to
the manufacturing year. For instance, the serial number 17-123456 has been manufactured in 2017.

The instrument manufacturer name is found on the label located on the back of the instrument.

Manufacturer name:
Vilber Lourmat SAS
BP31 - ZAC de Lamirault- Collegien
F-77601 Marne-la-Vallée Cedex 3
France
www.vilber.com

Vilber Lourmat is ISO-9001 certified since 1994.

CE conformity
This system complies with the requirements of the EC Directive 2004/108/EEC, 2006/95/EEC and EN 61010-1,
relating to Electro-magnetic compatibility and low voltage.
The Electro-magnetic susceptibility has been chosen at a level that gains proper operation in residential areas, on
business and light industrial premises and on small-scale enterprises, inside as well as outside of the buildings. All
places of operation are characterized by their connection to the public low voltage power supply system.

The CE marking and the corresponding EC Declaration of Conformity is only valid for the instrument when it is:
• used as a stand-alone unit, or
• connected to other products recommended or described in the user documentation, and
• used in the same state as it was delivered from Vilber Lourmat, except for alterations described in this user
documentation.

International standards
This product fulfills the requirements of the following standards:
IEC 61010 -Safety requirements for electrical equipment for measurement, control and laboratory use
EN 61326-1 - Electrical equipment for measurement, control, and laboratory use - EMC requirements

21
Recycling information
This product fulfills the requirements of the following standards:
2011/65/EU Annex II – Restriction of Hazardous Substances (RoHS) Directive
2012/19/EU – Waste Electrical and Electronic Equipment (WEEE) Directive

Decontamination
The equipment and the accessories must be clean from contaminants before decommissioning and all local
regulations must be followed with regard to waste disposal. Samples are to be disposed of according to local
regulations.

Please always enclose the Decontamination Declaration form for product return. We require the fully completed
decontamination declaration for accepting and processing the return. If it is not enclosed with the return, we will
return the product at your expense.

Recycling of UV lamps
The transilluminator and EPI UV lamps must be recycled or disposed of in a manner compliant with national and
local environmental regulations.

France only:
The buyer ensures and finances the decontamination, the collection and the disposal of waste electrical and
electronic equipment (WEEE) under the conditions provided in the Articles 21 and 22 of the Decree No. 2005-829
dated of 20 July 2005. In France, for tubes recycling, contact the Recylum, www.recylum.com Improper disposal may
be harmful to the environment and human health.

Disposal of electrical components

Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be
collected separately.
When taking the instrument out of service, the different materials must be separated and recycled according to
national and local environmental regulations.

22
E-BOX CX5 EDGE

About the E-BOX CX5 EDGE imaging system

The E-Box imaging system is a scientific instrument designed to capture fluorescence or colorimetric gel images. The
E-Box system uses the embedded version of the Bio-Vision software to control image capture and optimization for
selected applications

A scientific camera is used to capture high resolution digital images of protein and DNA bands in gels obtained by
electrophoresis separation methods. The instrument can capture images of fluorescent, and colorimetric samples,
depending on the system configuration.
The instrument can be used for research purposes in the academia and life sciences industry. The instrument cannot
be used for diagnostic purpose.

E-Box offers exquisite precision and resolution, which mean reliable results for both quantification and
documentation. The advanced imaging electronics has been developed by our experts especially for your scientific
applications. This association of our exclusive electronic, high-quality optics and advanced software delivers
outstanding performance. With E-Box, you simply reach the lowest limits of detection on all of your samples.

The E-Box system is supply with the PC version of the Bio-Vision software for image quantification and FTP access to
the E-Box system.

E-BOX CX5 EDGE system installation

Unpacking the system

WARNING
The E-Box imaging system is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before installing,
operating or maintaining the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or damage to
the instrument.

Please, open the E-BOX CX5 EDGE box carefully and verify the contents:
E-Box CX5 1
Power cable 1
Instruction manual 1
Bio-Vision software CD-Rom inside the instruction manual 1

 Remove carefully each component from the box.

23
 To remove the E-BOX CX5 EDGE from the box, please use the belts which surround the system and designed for
this purpose
 Do not handle the system by the camera or the camera support.
 Remove their protective plastic cover.
 Place the darkroom at its permanent location, the door facing forward. The cabinet has to be placed near the
computer used for image acquisition.

Protect the environment!

Dispose of packaging properly, according to existing and applicable waste management rules and regulations.

WARNING
It is recommended that the E-BOX CX5 EDGE darkroom be carried by at least two people – one
on each side- holding the instrument from the bottom side. Be sure that the door is properly
closed when carrying the instrument.

The imager weighs 40kg. Using two people, place the imager onto the benchtop given proper
space, electrical and environmental requirements. Remove the outer packaging materials.

24
WARNING
Do not connect the power cable to a power source until all connections are made. The power
source has to be grounded and protected by a circuit breaker.

WARNING
The E-BOX CX5 EDGE system is designed to fit a specific voltage. Please, check the voltage to
ensure it corresponds to the E-BOX CX5 EDGE specifications.

WARNING
Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air
circulation for the system. The system should be located in an area free of excessive dust or
moisture, strong magnetic fields or ionising radiation. It is also recommended that the
ambient temperature be stable and within the range of 15°C to 25°C (20°C recommended)
and that the relative humidity not exceed 70%, non-condensing.

WARNING
Ensure that all of the systems ventilation openings are free of interference. Excessive heat
build-up in the instrument may effect performance or cause operational failure.

The E-BOX CX5 EDGE system should be located away from water, solvents, or a corrosive
material, on a bench top that is dry and stable. The system should be placed away from
interfering electrical signals and magnetic fields. A dedicated electrical outlet should be used
to eliminate electrical interference from other instrumentation in your laboratory

WARNING
Do not defeat any instrument interlocks; they are designed to prevent user injury.

It is compulsory to power down the system and disconnect the AC mains from the unit before
performing any disassembly or repair to the system.

25
 UV WARNING
The use of the E-BOX CX5 EDGE involves ultraviolet (UV) illumination. Proper precautions
must be taken to avoid eye and skin exposure to the UV light. This instrument is meant for use
only by specialised personnel that know the health risks associated with UV radiation and the
chemicals that are normally used with this instrument.

Installing the hardware

Environmental conditions

Placement Place the E-BOX CX5 EDGE on a level-working surface.

The imager requires a stable laboratory bench or table capable of supporting 80 kg.

Provide a minimum clearance of 8cm at the rear of the imager to allow adequate
ventilation for camera cooling and access to the power cord and switch. In addition, allow
sufficient space to open the front-facing imager door to place sample within the imager.

The E-BOX CX5 EDGE is intended for indoor use only.

Free space LCD panel operation free space required: 50 cm

Right, left, rear and top minimum free space required: 10 cm

Other conditions Do not install the equipment near a window and avoid direct sunlight. We recommend
attached blinds to nearby windows

Do not install the instrument where it may get wet or flooded

Do not install the instrument in a dusty environment

Do not install the instrument in a place constantly or excessively exposed to vibration or

impacts (next to a centrifuge or to a compressor for instance)

Remove all hot or heat source near the E-Box CX5, specifically from the air intake.
Do not place objects near the power outlet to ensure easy access to the power cord for
disconnection in case of emergency.

Environmental Temperature: 18°C to 25°C

conditions Humidity: 20% to 70% RH (no dew condensation)

26
Imager back side

3
2

The system power supply and main switch are positioned at the back side of the instrument.
Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded
circuit capable of delivering the appropriate current and voltage is required for installation. Electrical requirements
(3) is located on the imager label on the rear panel of the imager
Switch on the main switch (2) to position 1 – on.
If required, switch off the main switch (2) to position 0 – off.

The main switch provides power to all of the imager components.

The imager is operated by an integrated computer, which is accessed through the touchscreen. The integrated
computer mother board connectors can be accessed from the back side of the display. When delivered to you, the E-
BOX CX5 EDGE camera and the darkroom control board USB connections are already plugged to the build-in
computer mother board.

The camera USB cable is connected to the mother board USB-3 port.

27
Four alternate USB ports could be used. A mouse and a keyboard could also be connected to these ports

.
For network connection, a LAN network port is also available.

E-BOX CX5 EDGE hardware operating instruction

Warning while using the darkroom

UV WARNING
Switch off the transilluminator when gel is not present on the UV filter. If the filter is too hot,
it will damage your electrophoresis gel.

UV WARNING
Wait for at least 20 second in the "High" position before reducing the intensity selector to
"Low”.

UV WARNING
If one or several tubes are off or used, and in order to keep a better homogeneity, we
recommend to change the 6 tubes simultaneously.

28
Imager rear side

The USB port of the left hand side of the instrument (7) is designed to hold your USB key drive for image saving.
The imager on / off switch (8) start or stop the E-BOX CX5 EDGE activates the internal computer, touchscreen and
thermoelectric camera.

Imager front side – Door open

The E-BOX CX5 EDGE SmartCab intelligent darkroom technology allows:
• Software control of the lighting
• Automatic white light intensity adjustment according to the sample position
• Pre-calibrated focus for all defined zoom preset

29
When the door is open, you can access the slide-out transilluminator, the door UV security switch (9) and the on/ off
button (8) of the transilluminator.

8 9

Press twice the transilluminator on/off button (8) to switch on the transilluminator light while the door is opened.
The white light switch on automatically when the door is opened.

Optional PadBox and Pad system

The PadBox multimodal container could accommodate our UV, blue, or white light pad or your own hardware such
as heater, cooler, electrophoresis tank, special light source etc. The power socket inside the darkroom is switched on
and off through the E-Box software.

The PadBox can easily integrate one of the several available Application Pad.

The PadConnect technology allows an automatic recognition of the installed Application Pad. Imaging and software
options are then adjusted accordingly.

Several Application-Pad can be easily inserted or removed inside the E-Box CX5:
• UV-Pad
• Super-Bright-Pad
• Sky-Pad: LED blue light transilluminator, 470nm

All Application Pad can be used as a standalone illumination device outside the E-BOX CX5 EDGE system, with an
optional plug adaptor.

30
31
Filter wheel
The E-BOX CX5 EDGE filter wheel is located inside the darkroom.

The 10 positions filter wheel is supplied with the F590 M58 interferential filter supplied with the system for Ethidium
bromide application. The F590 Ethidium bromide filter is attached to the position 1.
To select the filters, turn the black handle and select the position of your choice.

To install a filter, select the filter position using the handle and screw the filter in its dedicated holder.

32
E-BOX CX5 EDGE software interface operation
Introduction
This chapter describes how to operate the E-BOX CX5 EDGE imaging system.

Before to operate the E-BOX CX5 EDGE system, it is important that you have read and understood the safety
information. Make sure to read this User Guide relevant safety instructions before you start to operate the system.

WARNING
Do not operate E-BOX CX5 EDGE system in any other way than described in the User Manual.

The E-BOX CX5 EDGE instrument is a high-end ultra-sensitive scientific camera platform, designed to extract the
lowest level of detection from your protein or DNA sample. Our superior sensitivity, resolution and dynamics provide
optimal performance across a large array of applications.

The standard imaging process is as follows:

Start the E-BOX CX5 EDGE system & software

Position your sample

Capture an image

Visualize your image

Save and print

33
Start the E-BOX CX5 EDGE system & software

Switch on the E-BOX CX5 EDGE system

On the right hand side of the imager, press the power button to the position I, to turn it on.

After the computer has booted up completely, the following messages are displayed until the E-BOX CX5 EDGE
system is ready for image acquisition:

When system is ready, the start menu appears on the display:

34
Capture an image
This chapter describes the steps to acquire an image.

Fluorescence image acquisition

Fluorescence is the main method used for gene expression and protein detection. It results from a process that
occurs in molecules known as fluorophores. The fluorophore absorbs the excitation light, reaching a higher energy
state. By returning to its former state, it emits fluorescent light.
The E-BOX CX5 EDGE system separates the emitted light from the excitation light in order to obtain an optimum
sample image. According to the configuration, the E-BOX CX5 EDGE can accommodate several excitation and
emission channels in the visible, RGB and UV area and is ideal for a large array of applications such as 1D DNA gel, 1D
protein colorimetric samples, multiplexing, stain free gel and blot.

Image capture workflow

The basic steps to acquire an image are:

Choose the application type

Acquire the image

Save or print the image

35
Choose the application type

The Application selector

The E-BOX CX5 EDGE runs with pre-defined image acquisition parameters to facilitate the use of the system for a
specific application. These pre-defined parameters are gathered in the E-BOX CX5 EDGE Application Protocol and can
be selected from the Application Selector:

The E-BOX CX5 EDGE Application protocol will ease the imaging workflow by automatizing the exposure parameters
adjustment. The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular
basis. It stores the following information:
• The image exposure pre-defined set-up;
• The live preview pre-defined parameters;
• The image display pre-defined set-up;
• The image printing pre-defined set-up;
• The image file pre-defined set-up.

The benefits of the Application protocol are as follows:

• Time saving
• Reproduction of image acquisition parameters
• Protocols are modifiable, allowing the user to maintain an original template while modifying it for a slightly
different result, with minimal effort

The factory settings include several pre-defined Application protocols. You can also create your own Application
protocol. Please refer to the Application protocol chapter of this User Guide to have more details on the way you can
create your own Application.

36
For a UV excited gel sample, select the UV fluorescence application from the Application Selector window:

For a colorimetric sample using white light conversion screen, select the Conversion screen application from the
Application Selector window:

For a colorimetric sample using epi white light (light from the top), select the White light application from the
Application Selector window:

Acquire the image

The E-BOX CX5 EDGE system has three exposure mode:
• The live mode
• The auto mode
• The start mode

The exposure modes are gathered in the exposure mode menu:

The Live mode

Live mode allows direct visualization of the image. This mode enables you to adjust the focus, and to position your
sample.

Note: A live image means the image displayed is refreshed every 1/20th of a second. This short exposure time (or
frame) is adequate for a variety of white light samples including protein gels and autoradiography. A live image,
however, is not sufficient for most samples, which are visualized and photographed over a relatively dim UV light
source. A feature called integration compensates the low light situation by allowing the camera to obtain a timed
exposure.

Note: After 2 minutes, the software will automatically stop the live preview.
Note: The Preview mode is designed to help for sample positioning and focusing. To this extend, the Preview mode
is always with white light EPI illumination.

The selection of the Live mode will open the following menu:

37
Enter the duration of the exposure.
Define your exposure time using the arrow buttons:

Modify the zoom and focus settings.

The Zoom in allows you to change the size of your sample, on the image. To proceed, click on the Arrow buttons. The
zoom automatically moves to the new value. The zoom control will modify the focus accordingly to keep a sharp
image.

The Focus is needed to adjust the sharpness of the image by changing the focal point of the lens. To proceed, click on
the Arrow buttons.
You can reset the values to the one saved in the user profile. You can also save the new values in your user profile.

The 3D Scan images your sample in real time and reconstructs the data to create live three dimensional model.

The 3D reconstruction provides direct information regarding the image dynamic, background level and protein or
DNA quantity. A little change of exposure time will refresh the 3D view automatically. The saturation effect can be
controlled live before the image is frozen.

38
Click on “Saturation” to displays the saturated pixels in red.

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.
A 16-bit image has a maximum grey level of 65535

Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image
Note: A saturated image creates quantification error when studied by an image analysis software. Decrease the
exposure time to avoid saturation.

Case 1: Case 2:

Original display Same image with the saturated pixels

indicated in red

39
Click on Stop to finalize the image acquisition process:

The Auto mode

The E-BOX CX5 EDGE system calculates automatically the optimum exposure time (Auto-exposure). When you select
the Auto-Exposure option, the system samples the light levels and uses the values to calculate the final exposure
time.
To set the auto-exposure mode, select Auto from the exposure mode menu:

The Start mode

The E-BOX CX5 EDGE system requires the user to specify the exposure time.
To set the exposure time, select Manual from the exposure mode menu:

The selection of the Start mode will open the following menu:

Enter the duration of the exposure.

Define your exposure time using the arrow buttons:

40
Note: The integration time increase or decrease by 40 milli-seconds, except for 10 and 20ms.

Note: With long integration time, a delay could be necessary before an image is displayed on the monitor (up to
twice the selected Exposure time).

Note: When the specified exposure time is reached, the last captured image is displayed. The camera continues to
integrate the image on the sensor, updating the display whenever the specified Exposure time is reached. The Stop
exposure button stops the exposure process. The last full exposure is displayed.

You can also modify the zoom and focus settings, display the 3D image and the saturation. More details are provided
in the Live mode chapter.

The image dynamic and the exposure time is displayed in the control bar on top of your sample image:

Image dynamic:
The image dynamic is crucial for quantitative accuracy. It should to be the largest possible within the gray scale of
the system.
 65 535 gray levels for 16-bit imaging

The gray scale is the total number of levels between white and black. The image dynamic range within this scale.
Adjustments are necessary to obtain an image with the broadest dynamic. With most of the gels, a real time image
has a limited dynamic. In this case, the exposure time enhances the image dynamic.

In order to obtain an optimum image, you can control the image dynamic by adjusting the exposure time.

Click on Stop to finalize the image acquisition process:

41
Save or print the image

Clicking on Save will start the saving of the captured image. The image is saved on the USB® key drive, according to
the default image format.

The E-Box has an internal memory, interrelated with the USB external saving drive. When pressing the “Save” key,
the image is saved at the same time both on the internal memory and on the USB key drive.

You can access the internal memory through the E-Box FTP server.

Note: The image file is named by the system. The default image name has 8 characters. The first 2 are IM. The last 6
are a number ascending from 000001 to 999999.

Note: The default file name is incremented by one each time an image is saved.

Note: The file extension varies according to the image file format:
 For TIFF, the extension is TIF
 For BMP file, the extension is BMP;
 For JPEG, the extension is JPG

42
To print, press the “Print” key:

The image is printed on the default printer, if defined.

Note: a printer message can appear on the display in the case of lack of ink or paper. In such case the message
should disappear after few seconds. If not, please press any touch from the panel and the message will disappear
after few seconds.

Inverse
Click on “Inverse” to inverse the grey level of the image. This makes a negative image.
Case 1: Case 2:

Positive image Inverted image - Negative image

(white spot, black background) (black spot, light background)

43
Adjust the image display

The Display option menu is helpful to enhance the image display by modifying the image greyscale selection to be
displayed. In standard, the E-BOX CX5 EDGE images have 16-bit format and Windows® can only display 8-bit images
(256 grey levels). Due to this limitation, the software handles two images:
• A “memory” image corresponding to the 16-bit format (65 536 grey levels)
• A “displayed image” corresponding to the image displayed on the screen (256 grey levels)

The easiest way to calculate the “display image” would be to translate the full grey scale each time an image is
acquired: the x grey levels values of the “memory” image corresponds to 256 values in the displayed image. In that
case, it won’t be possible to visualize faint spots on a dark image.

The software offers the possibility to select the grey level range to translate for the display image calculation. All the
grey levels under the “Min value” defined will be converted to 0 (Black) in the displayed image. All the grey levels
upper the “Max Value” defined will be set to 255 (White) in the displayed image. The grey levels between those two
limits will be converted in an intermediate grey level value following a linear rule.

You can select one from the 5 image display presets:

The image displays presets goes from the minimum image grey levels to 20%, 40%, 60% or 100% of the image depth.

44
 Case 1: Case 2:

Default optimum display Optimum display enhancement

The image appears brighter. The faint
bands are more visible.

Note: The Display function inform you of the minimum and of the maximum grey level obtained on the image.

Note: The Display has no impact on the analysis. Only the display of the image is modified.

45
The Application protocol
What is an Application protocol?
The software runs with pre-defined image acquisition parameters to facilitate the use of the system for a specific
application. These pre-defined parameters are gathered in the software Application protocol.

The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular basis. It
stores the following information:
- The image exposure pre-defined set-up;
- The live preview pre-defined parameters;
- The image display pre-defined set-up;
- The image printing pre-defined set-up;
- The image file pre-defined set-up.

The factory settings include several pre-defined Application protocols

You can also create your own Application protocol.

The benefits of the Application protocol are as follows:

- Time saving
- Reproduction of image acquisition parameters
- Protocols are modifiable, allowing the user to maintain an original template while modifying it for a slightly
different result, with minimal effort

How to access the application protocol?

Select an application protocol from the application menu. Click on the application name to open the application
protocol menu.

46
The Application protocol can either be displayed with a summary view or with a complete view. By default, the
application protocol is displayed with the summary view. Click on Advanced options to have access to all the
application protocol features.

Advanced exposure – Exposure predefined set-up

The Advanced Exposure folder contains the exposure pre-defined set-up:

Typical Exposure set-up for a fluorescence application

• Zoom options
The standard mode allows you to use the E-Box in a portrait mode.

47
You can select till 6 zoom preset positions. Position 1 correspond to a field of view of roughly.

Zoom options are as followed:

Approximate field of view according to the zoom mode:

Zoom options
1 260x200mm
2 200x170mm
3 170x130mm
4 140x110mm
5 100x80mm
6 80x55mm

• Lighting (Fluorescence mode only)

This option defines the lighting used for the image exposure. All E-Box systems are equipped with LED Visible light.
You can select between low and high intensity.

Note: The lighting options depend on your system configuration. UV, or blue lighting could be available. The
selection of these lighting options will be done from this menu.

• Extended resolution
The effective pixels technology allows the extension of the camera native image resolution by software
interpolation.

Note: This process requires important computer calculation and sometime could be necessary before the extended
image is displayed.

• Fast mode / Standard mode

The fast mode provides lower image resolution to speed-up the imaging process.

• Auto-exposure set-up

48
Test time
In order to calculate an ideal exposure time, the software needs to analyze a test image, taken in binning mode with
a test exposure time. Then it searches for the minimum and maximum acceptable grey levels and calculate the
exposure time so the highest grey levels values of the final image reach a maximum defined (Saturation Value).

Note: in the case the auto-exposure does not give you entire satisfaction for your specific application, we
recommend increasing the test time.

Auto mode
The Auto mode options allow you to select between a standard auto exposure time, an extended auto exposure
time or a reduced auto-exposure time.

Standard exposure is optimized for all bands.

Over exposure (faint band) results in a longer exposure where faint bands are more visible, but more prominent
bands may be over exposed.
Under exposure (bright band) results in a shorter exposure where bright bands are more visible, but more prominent
bands may be under exposed.
User defined allows you to decide of the over exposure or under exposure settings. For instance, 150% will increase
the standard exposure time by 1.5.

49
• Manual exposure set-up

Manual exposure set-up

This option defines the default imaging exposure time. The software has two exposure time scales:
- One for short times: 40 milli-second to 4 seconds
- One for long times: 4 sec to 1 minute

Note: With the short integration time scale, the integration time increases or decreases by 40milli seconds.
Note: With long integration time, a delay could be necessary before an image is displayed on the monitor (up to
twice the selected Exposure time).

50
Preview – Live preview pre-defined parameters
Preview folder contains the live review pre-defined set-up:

• Extended resolution
The effective pixels technology allows the extension of the camera native image resolution by software
interpolation.

Note: This process requires important computer calculation and sometime could be necessary before the extended
image is displayed.

• The lens prefixed zoom position table

The default positions are part of the system factory settings. Nevertheless, you can save your owned position using
the zoom menu you can access during image acquisition.

The lens prefixed position table summarizes the default focusing preset per zoom option for the concerned
Application protocol, when modified by the user.

51
Display – Image display pre-defined parameters
The Display folder contains the image display pre-defined set-up:

• Select display mode

The Select display mode options allow you to select between a standard display, an enhance display for the faint
band and an enhance display for the bright band.

Standard display is optimized for all type of image.

Enhance display for faint band ensure the faint bands are more visible, but more prominent bands will look very
strong.

Enhance display for bright band ensure the bright bands are more visible compared to their background. This is well
adapted for fluorescence sample.

• Set the gamma value

Gamma adjustment corrects an image by creating a new version of the original. To create the new image, the
Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the following
graph:

52
The above graph demonstrates the basic principles of gamma adjustment:
- Black (pixel value = 0) remains black at all gamma values.
- White (pixel value = full scale) remains white at all gamma values.
- Gamma values greater than one lift the darker areas of the original image into the brighter areas of the new image.

A gamma curve is smooth: there are no unexpected jumps or cut-offs. This means that when viewing a gamma
adjusted image, you will be able to see the details (intensity differences) in both the black and white areas of the
image.

When the bright areas of these types of images are correctly exposed, the darker areas can be so dark that they are
in effect invisible. Gamma Adjust can remedy this problem. The gamma adjustment results in a better display of
detail by lightening the darker areas without burning out bright areas or lightening black areas:

• Inverse
Select “Inverse” to inverse the grey level of the image. This makes a negative image.

• Display the saturation

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.

The maximum grey level depends of the bit-depth. For instance:

A 16-bit image has a maximum grey level of 65535

Select the saturation option and the saturated pixels are displayed in red:

Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image

Note: A saturated image creates quantification error when studied by an image analysis software. Gel-doc systems
have to indicate to the user if the image is saturated and if it is then necessary to modify the integration time.
53
• Select the color palette
The pseudo colors can display different types or levels of fluorescence in an image. It replaces the original grey levels
of the image by another palette color. The software has several predefined palette designs. Select your palette
design from the followings:

Printing – Image printing pre-defined parameters

The Printing folder contains the image printing pre-defined set-up:

• Print with image name

Print the image with its name.

• Print with date and time

Print the image with the computer time.

• Print out text color

Select the color of the text printing.

• Free text print-out

You can select a free text (for instance the name of your institute), the image name (if the image has been previously
saved) and date and time.
The text is then displayed below the image in the print out:

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TUESDAY, APRIL 27, 2017 – 7.05AM

General – Image file pre-defined parameters

The General folder contains the image file pre-defined set-up

•User name
You can define the name of a user for an application protocol. The user could be the name of a working group, the
name of a user or a subtitle to the name of the application.

•Protocol’s avatar
To simplify the reconnaissance of the Application’s protocol, an icon is provided to differentiate the application by a
color.

•Default file name / Image name prefix

The default file name could either be based on the Date and Hour the image has been taken or by a prefix. You can
decide of the default generic file name to simplify the file name generation. By default, the name is IM00000x. The
image could also be named according to the time and hour it has been taken.

•File format
Images can be saved in CompatibilityPlus TIFF scientific image format (16-bit format recommended), TIFF scientific
image format (16-bit format), BMP (8-bit format only), JPEG (compressed) or APX (proprietary file format – 16-bit
format). This option allows you to select the default file format.

55
•Preferred image saving directory
This option defines the directory where the image is saved by default
Alternatively, you can select to always open the last used directory by clicking on the following option:

•Autosave
Use the Image Auto-Save feature to automatically save images as part of the capture process, in the defined image
saving directory.

•Preferred settings saving directory

This option defines the directory where are saved the configuration files.

Select to automatically activate the post processing after the image acquisition.

Note: Please refer to the Processing menu to have more details on the post process working principles.

The Apps studio

The Apps Studio is an innovative library of applications which contains more than 40 different protocols for your
blot, gel and other bioluminescence samples. The protocols are easily accessible and gathered into four master
protocols: DNA/RNA gels, Protein gels, Protein blots, Bioluminescence and others.

The Studio contains the excitation and the emission spectra of the main fluorophores used in modern molecular
biology laboratory. It also suggests the best possible system configuration in terms of excitation light source,
emission filter and sensitivity level. The Apps Studio ensures reproducibility and one click image acquisition for the
best ease of use.

You can access the Apps Studio by clicking on the Apps Studio tab from the Application protocol menu:

56
The applications library is organized into 3 chapters:
- Fluorescence
- Chemiluminescence
- Bioluminescence

Select your chapter and then your application, you can then view the imaging application protocol and load the user
profile if you have the appropriate configuration.

57
The System settings
Settings feature allows the adjustment of the system settings such as the filter controls or the lens calibration. Click
on “Settings” from the menu barre:

A window displays the following menu:

The Settings menu contains the following features, related to the E-Box system:

58
 General Displays the E-Box information such as the system option, the software
version, the filters installed and the lens calibration state.

Language selection Select the language you want the software to run if the language pack is
installed in your system

Mail to Input the mail receiver address

Windows parameters Set the E-Box hardware and software parameters such as the network
address

Shutdown system Shutdown the system

The general system settings

The General header from the Settings menu summarizes the information concerning your system and its set-up. It
gives you access to the following information:
 Systems name & version
 Camera and darkroom state
 Options installed
 Calibration stat

To proceed, select the General header from the Settings menu.

Language selection
The software could run in English, in German, and in Chinese if the language pack is installed in your system. Select
your language from the list. To proceed, select the Language selection header from the Settings menu:

59
Then select the appropriate language from the pop-up windows:

You will be prompt to restart the software for the change to occur.

To close the Settings menu, click on or

60
Mail to
Manage the email receiver address from the Mail to screen.

You can either use the default system SMTP or your own SMTP settings.

In such case, you need to define the following parameters:

• “Your Name” is the name that other people will see when you send them e-mail. It should usually be your real
name, such as Bill Gates or Winston Churchill.
• Enter your “Email Address”. The address must be the e-mail address of the mailbox you created in your account
management control panel.
• Enter SMTP “Outgoing mail server” as well as the port you are using.
• Enter your e-mail address again as the “User Name”. (The user name must be all lowercase).
• Type your e-mail password into the “Password” box. This is the password you chose for this e-mail address
when you created the mailbox in your account management control panel. This password may be different from
your master “My Account” password with our company.
• Do not click “Test Account Settings” or “Next” yet; they will not work until you complete the next steps.

When finished, please test the account settings by using the Test parameters option.

61
Windows parameters
You can update or reset, lock or unlock the system and set its network address from the Windows Parameters
screen:

Lock / unlock the E-Box system

The E-BOX CX5 EDGE system is write-protected to prevents the installation of any none approved programs or files
such as malware or virus. Thanks to the write-protected function, all files that are not write-protected are removed
with a restart of the system. In other words, a simple reboot will remove any malware or virus that would have been
able to infect the E-Box system.

The Lock/Unlock function is designed to unlock the system to allow the installation of additional drivers (such as
printer drivers or wireless network device).

With the Unlock function, the write-protection is disabled after the system is restarted. At that time, you can install
the needed drivers. Upon reboot, the system will have the new drivers installed, and will be write-protected again.

Before to add a device, it is compulsory to scan for virus the USB key drive which will contain the driver to be
installed in the E-Box system.

Add a printer

Note: You need to unlock the system to add a printer

To proceed, select the Control Panel function:

62
Select control panel button, a password is required to access the function, please request it to the manufacturer

Select “Devices and printer” and then “Add a printer”. Then follow instructions displayed on screen.

Once the installation done, restart the system to switch it back to “Locked” mode

Set the network parameters

Note: You need to unlock the system to set the network parameter

The E-Box can be connected to your computer through the local area network (LAN). A LAN is a communication
network connecting a group of computers, printers, and other devices located within a relatively limited area (for
example a laboratory). A LAN allows any connected device to interact with any other on the local network. For
instance, any computer connected on the LAN could download an image.

Transmission Control Protocol/Internet Protocol (TCP/IP) is the most popular network protocol, and the basis for the
Internet. In Windows operating system, TCP/IP is automatically installed.

On a TCP/IP network, you must provide IP addresses to clients. This means, you need to provide an address to make
sure your E-Box is already properly connected to your LAN and that an IP address is assigned to the E-Box.

To proceed, select the Control Panel function:

63
By default, we recommend to select Automatic TCP/IP parameters (automatic IP address assignment from your
network):
Fill-in the E-Box IP address, subnet mask and gateway with the IP address defined in the E-BOX CX5 EDGE system.

Then, validate by clicking on OK.

64
Connect to the E-Box from your computer

Connect using your internet browser

Retrieve an image directly from your internet browser (FireFox, Explorer, Safari, …)
• The E-Box IP address parameter is set to the DNS mode:
If the E-Box is connected in the same IP domain as your computer, you can download your image directly from your
internet browser by using the following address: ftp://images

• DNS is not selected and you use a defined IP address

If the E-Box is connected in the same IP domain as your computer, you can download your image directly from your
internet browser by using the following address: ftp://YOUR DEFINED E-BOX IP ADRESS (for instance ftp://10.0.0.27).

Connect using the Bio-Vision software

The Bio-Vision software helps you to download your image from the E-Box system.

First, you need to activate the E-Box function from the Bio-Vision software. To proceed, select the Settings window
and activate the “Display connection to E-Box unit” option:

You can connect your E-Box to the local area network of your laboratory and download your gel image from the
comfort of your office.

65
The E-Box has a unique IP address which allows you to connect the system to your laboratory network for
downloading. The image acquisition is performed in your laboratory and the image is saved in the internal memory
of the E-Box. Then, from your office, you can easily download the image using your Bio-Vision software.
You access the Connect to E-Box function thanks to the Exposure menu:

Then, click on the Connect to E-Box button to access the function options:

To do so, you need first to set an IP address and a subnet address for your E-BOX CX5 EDGE system.

Connection parameters
Bio-Vision provides connectivity between your computer and the E-Box through the local area network (LAN) on
which is connected your E-Box system. A LAN is a communication network connecting a group of computers,
printers, and other devices located within a relatively limited area (for example a laboratory). A LAN allows any
connected device to interact with any other on the local network. For instance, any computer connected on the LAN
could download an image.

Transmission Control Protocol/Internet Protocol (TCP/IP) is the most popular network protocol, and the basis for the
Internet. In Windows XP TCP/IP is automatically installed.

On a TCP/IP network, you must provide IP addresses to clients. This means, you need to provide an address to make
sure your computer is already properly connected to your LAN and that an IP address is assigned to the E-Box.

The remote “connect to the E-Box” from your computer must be configured to permit users to request the specific
E-Box IP address. Connections are created and configured thanks to the Bio-Vision IP address.

66
For remote access to the E-Box from your computer, you need to set the E-Box IP address in the setup Bio-Vision
software. To proceed, click on Define connection parameters:

A pop-up window displays the following menu:

E-BOX CX5 EDGE IP address

By default, we recommend to select DNS (automatic IP address assignment from your network):

If this option is not working properly, unselect the DNS option:

Fill-in the E-Box IP address with the IP address defined in the E-BOX CX5 EDGE system.

Then, validate by clicking on OK.

E-Box need password

This option is not used with the E-Box CX5. The older E-Box system required a password for the ftp access. If you are
using an old version of the E-Box system, please select E-Box need password for your ftp access. The password is
then e-box.

Connect to the E-Box:

To download the image from the E-Box, click on Connect to the E-Box unit button:

A pop-up windows prompt you the system is connecting to the E-Box:

67
The list of the E-Box images appears in the table:

Select your image. An image thumbnail appears. Click on Download selected image(s) to download your image and
open it in the Bio-Vision software.

68
How to map a network drive
Note: Please do not create any other Windows user account in the system.

Note: You can connect a mouse and keyboard to facilitate the operations.

Note: You might need the system access password. Please contact your dealer to get this password.

Switch to “Unlock” mode

Go to settings, then select “Windows parameters” and click on “Lock/unlock windows” option

Once the system has rebooted in “unlock mode”, run the Windows Explorer: in Settings>Windows parameters, click
on “Show Window Explorer” button

Enter the system password and click on OK, to access the Widows file Explorer

Select “Map network drive” option

69
The following dialog box is displayed

Please insure that both “Reconnect at logon” and “connect using different credentials” options are checked.
Then click on “Browse” button to define the Network driver location

1- Browse in your network and define the correct emplacement (“Echanges” in this example), then click on OK

2- The network Login and password are then requested (this your Login parameters, not the E-Box’s one). The
E-Box will use those parameters to connect to the drive.

70
Click on OK to validate, the new drive is mapped

3- Close the Explorer Window, in order to insure that the parameters are kept in E-Box’s unit. Reboot the unit
in “unlocked” mode:
a) in “Settings” Window, select “Shutdown Window”.
b) Once the system is down, restart it with the on/off switch.
E-Box should restart in “unlocked” mode, insure that the network drive is still correctly mapped with
Windows Explorer (Step 1 to 3)

71
4- Once the mapping confirmed, click on “Restart in Standard mode” button to reboot the system and acquire
images.

72
Update the E-Box firmware
This procedure can only be done with the manufacturer approval. To proceed, you will need the firmware files from
Vilber.
To install, please unzip the Vilber file to have in the root directory of your USB stick the following files:
• Setup.exe
• Setup.zip
Plug the USB stick to the E-Box.
Then, follow the E-Box system update procedure using the settings function:
Configuration / Settings / Windows parameter / Software update
Select the USB stick directory and the Setup.exe file, then OK.

Do not remove the USB stick or switch off the system till the process is finalized. The process could last roughly 5
minutes.

73
Export system
You can export in a text file the system details. This could be necessary for service investigation.

Run TeamViewer for premium service support

TeamViewer is a proprietary computer software for remote control and file transfer between computers.
For customer support, under your instruction, TeamViewer generates a session password (one-time password). If
your tells us your password, we can connect to your computer with your invitation.
No one can access your computer without your invitation and without knowing the session password.

Note: Your computer need to be connected to the network.

Click on Run TeamViewer.

The TeamViewer window will open.

74
INFINITY CX5

About the INFINITY CX5 EDGE imaging system

The INFINITY imaging system is a scientific instrument designed to capture fluorescence or colorimetric gel images.
The INFINITY system uses the Bio-Vision software to control image capture and optimization for selected applications.

A scientific camera is used to capture high resolution digital images of protein and DNA bands in gels obtained by
electrophoresis separation methods. The instrument can capture images of fluorescent, and colorimetric samples,
depending on the system configuration.
The instrument can be used for research purposes in the academia and life sciences industry. The instrument cannot
be used for diagnostic purpose.

INFINITY offers exquisite precision and resolution, which mean reliable results for both quantification and
documentation. The advanced imaging electronics has been developed by our experts especially for your scientific
applications. This association of our exclusive electronic, high-quality optics and advanced software delivers
outstanding performance. With INFINITY, you simply reach the lowest limits of detection on all of your samples.

INFINITY CX5 EDGE system installation

Unpacking the system

WARNING
The INFINITY imaging system is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before installing,
operating or maintaining the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or damage to
the instrument.

Please, open the INFINITY CX5 EDGE box carefully and verify the contents:
INFINITY CX5 1
Power cable 1
USB cable 2
Instruction manual 1
Bio-Vision software CD-Rom inside the instruction manual 1

 Remove carefully each component from the box.

 To remove the INFINITY CX5 EDGE from the box, please use the belts which surround the system and designed for
this purpose
 Do not handle the system by the camera or the camera support.
 Remove their protective plastic cover.
 Place the darkroom at its permanent location, the door facing forward. The cabinet has to be placed near the
computer used for image acquisition.
75
WARNING
It is recommended that the INFINITY CX5 EDGE darkroom be carried by at least two people –
one on each side- holding the instrument from the bottom side. Be sure that the door is
properly closed when carrying the instrument.

The imager weighs 40kg. Using two people, place the imager onto the benchtop given proper
space, electrical and environmental requirements. Remove the outer packaging materials.

WARNING
Do not connect the power cable to a power source until all connections are made. The power
source has to be grounded and protected by a circuit breaker.

WARNING
The INFINITY CX5 EDGE system is designed to fit a specific voltage. Please, check the voltage
to ensure it corresponds to the INFINITY CX5 EDGE specifications.

WARNING
Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air
circulation for the system. The system should be located in an area free of excessive dust or
moisture, strong magnetic fields or ionising radiation. It is also recommended that the
ambient temperature be stable and within the range of 15°C to 25°C (20°C recommended)
and that the relative humidity not exceed 70%, non-condensing.

WARNING
Ensure that all of the systems ventilation openings are free of interference. Excessive heat
build-up in the instrument may affect performance or cause operational failure.

The INFINITY CX5 EDGE system should be located away from water, solvents, or a corrosive
material, on a bench top that is dry and stable. The system should be placed away from
interfering electrical signals and magnetic fields. A dedicated electrical outlet should be used
to eliminate electrical interference from other instrumentation in your laboratory

76
 WARNING
Do not defeat any instrument interlocks; they are designed to prevent user injury.

It is compulsory to power down the system and disconnect the AC mains from the unit before
performing any disassembly or repair to the system.

UV WARNING
The use of the INFINITY CX5 EDGE involves ultraviolet (UV) illumination. Proper precautions
must be taken to avoid eye and skin exposure to the UV light. This instrument is meant for use
only by specialised personnel that know the health risks associated with UV radiation and the
chemicals that are normally used with this instrument.

Installing the hardware

Environmental conditions

Placement Place the INFINITY CX5 EDGE on a level-working surface.

The imager requires a stable laboratory bench or table capable of supporting 80 kg.

Provide a minimum clearance of 8cm at the rear of the imager to allow adequate ventilation
for camera cooling and access to the power cord and switch. In addition, allow sufficient
space to open the front-facing imager door to place sample within the imager.

The INFINITY CX5 EDGE is intended for indoor use only.

Other conditions Do not install the equipment near a window and avoid direct sunlight. We recommend
attached blinds to nearby windows

Do not install the instrument where it may get wet or flooded

Do not install the instrument in a dusty environment

Do not install the instrument in a place constantly or excessively exposed to vibration or

impacts (next to a centrifuge or to a compressor for instance)

Remove all hot or heat source near the INFINITY CX5, specifically from the air intake.

Do not place objects near the power outlet to ensure easy access to the power cord for
disconnection in case of emergency.

77
 Environmental Temperature: 18°C to 25°C
conditions Humidity: 20% to 70% RH (no dew condensation)

Imager back side

The system power supply and main switch are positioned at the back side of the instrument.
Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded
circuit capable of delivering the appropriate current and voltage is required for installation.
Electrical requirements is located on the imager label on the rear panel of the imager
Switch on the main switch to position 1 – ON.
If required, switch off the main switch to position 0 – OFF.

The main switch provides power to all the imager components.

The darkroom control board USB cable goes from the darkroom to the computer USB port.

Computer rear side - Connection

Do not connect the camera USB cable and the darkroom camera cable before the software installation

78
INFINITY CX5 EDGE hardware operating instruction
Warning while using the darkroom

UV WARNING
Switch off the transilluminator when gel is not present on the UV filter. If the filter is too hot,
it will damage your electrophoresis gel.

UV WARNING
Wait for at least 20 second in the "High" position before reducing the intensity selector to
"Low”.

UV WARNING
If one or several tubes are off or used, and in order to keep a better homogeneity, we
recommend to change the 6 tubes simultaneously.

Imager front side – Door open

The INFINITY CX5 EDGE SmartCab intelligent darkroom technology allows:
• Software control of the lighting
• Automatic white light intensity adjustment according to the sample position
• Precalibrated focus for all defined zoom preset

When the door is open, you can access the slide-out transilluminator, the door UV security switch (9) and the on/ off
button (8) of the transilluminator.

79
 8 9

Press twice the transilluminator on/off button (8) to switch on the transilluminator light while the door is opened.
The white light switch on automatically when the door is opened.

Optional PadBox and Pad system

The PadBox multimodal container could accommodate our UV, blue, or white light pad or your own hardware such
as heater, cooler, electrophoresis tank, special light source etc. The power socket inside the darkroom is switched on
and off through the software.

The PadBox can easily integrate one of the several available Application Pad.

The PadConnect technology allows an automatic recognition of the installed Application Pad. Imaging and software
options are then adjusted accordingly.

Several Application-Pad can be easily inserted or removed inside the INFINITY CX5:
• UV-Pad
• Super-Bright-Pad
• Sky-Pad: LED blue light transilluminator, 470nm
• White-Light- Pad: LED white light transilluminator

All Application Pad can be used as a standalone illumination device outside the INFINITY CX5 EDGE system, with an
optional plug adaptor.

80
Filter wheel
The INFINITY CX5 EDGE filter wheel is located inside the darkroom.

The 4 positions filter wheel is supplied with the F590 M58 interferential filter supplied with the system for Ethidium
bromide application. The F590 Ethidium bromide filter is attached to the position 1.
To select the filters, turn the black handle and select the position of your choice.

To install a filter, select the filter position using the handle and screw the filter in its dedicated holder.

81
QUANTUM CX5

About the QUANTUM CX5 imaging system

The QUANTUM imaging system is a scientific instrument designed to capture fluorescence or colorimetric gel
images. The QUANTUM system uses the Bio-Vision software to control image capture and optimization for selected
applications.

A scientific camera is used to capture high resolution digital images of protein and DNA bands in gels obtained by
electrophoresis separation methods. The instrument can capture images of fluorescent, and colorimetric samples,
depending on the system configuration.
The instrument can be used for research purposes in the academia and life sciences industry. The instrument cannot
be used for diagnostic purpose.

QUANTUM offers exquisite precision and resolution, which mean reliable results for both quantification and
documentation. The advanced imaging electronics has been developed by our experts especially for your scientific
applications. This association of our exclusive electronic, high-quality optics and advanced software delivers
outstanding performance. With QUANTUM, you simply reach the lowest limits of detection on all of your samples.

QUANTUM CX5 system installation

Unpacking the system

WARNING
The QUANTUM imaging system is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before installing,
operating or maintaining the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or damage to
the instrument.

Please, open the QUANTUM CX5 box carefully and verify the contents:
QUANTUM CX5 1
Power cable 1
USB cable 2
Instruction manual 1
Bio-Vision software CD-Rom inside the instruction manual 1

 Remove carefully each component from the box.

 To remove the QUANTUM CX5 from the box, please use the belts which surround the system and designed for
this purpose
 Do not handle the system by the camera or the camera support.
 Remove their protective plastic cover.

82
 Place the darkroom at its permanent location, the door facing forward. The cabinet has to be placed near the
computer used for image acquisition.

WARNING
It is recommended that the QUANTUM CX5 darkroom be carried by at least two people – one
on each side- holding the instrument from the bottom side. Be sure that the door is properly
closed when carrying the instrument.

The imager weighs 40kg. Using two people, place the imager onto the benchtop given proper
space, electrical and environmental requirements. Remove the outer packaging materials.

WARNING
Do not connect the power cable to a power source until all connections are made. The power
source has to be grounded and protected by a circuit breaker.

WARNING
The QUANTUM CX5 system is designed to fit a specific voltage. Please, check the voltage to
ensure it corresponds to the QUANTUM CX5 specifications.

WARNING
Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air
circulation for the system. The system should be located in an area free of excessive dust or
moisture, strong magnetic fields or ionising radiation. It is also recommended that the
ambient temperature be stable and within the range of 15°C to 25°C (20°C recommended)
and that the relative humidity not exceed 70%, non-condensing.

WARNING
Ensure that all of the systems ventilation openings are free of interference. Excessive heat
build-up in the instrument may affect performance or cause operational failure.

The QUANTUM CX5 system should be located away from water, solvents, or a corrosive
material, on a bench top that is dry and stable. The system should be placed away from
interfering electrical signals and magnetic fields. A dedicated electrical outlet should be used
to eliminate electrical interference from other instrumentation in your laboratory

83
 WARNING
Do not defeat any instrument interlocks; they are designed to prevent user injury.

It is compulsory to power down the system and disconnect the AC mains from the unit before
performing any disassembly or repair to the system.

UV WARNING
The use of the QUANTUM CX5 involves ultraviolet (UV) illumination. Proper precautions must
be taken to avoid eye and skin exposure to the UV light. This instrument is meant for use only
by specialised personnel that know the health risks associated with UV radiation and the
chemicals that are normally used with this instrument.

Installing the hardware

Environmental conditions

Placement Place the QUANTUM CX5 on a level-working surface.

The imager requires a stable laboratory bench or table capable of supporting 80 kg.

Provide a minimum clearance of 8cm at the rear of the imager to allow adequate ventilation
for camera cooling and access to the power cord and switch. In addition, allow sufficient
space to open the front-facing imager door to place sample within the imager.

The QUANTUM CX5 is intended for indoor use only.

Other conditions Do not install the equipment near a window and avoid direct sunlight. We recommend
attached blinds to nearby windows

Do not install the instrument where it may get wet or flooded

Do not install the instrument in a dusty environment

Do not install the instrument in a place constantly or excessively exposed to vibration or

impacts (next to a centrifuge or to a compressor for instance)

Remove all hot or heat source near the QUANTUM CX5, specifically from the air intake.

Do not place objects near the power outlet to ensure easy access to the power cord for
disconnection in case of emergency.
84
 Environmental Temperature: 18°C to 25°C
conditions Humidity: 20% to 70% RH (no dew condensation)

Imager back side

The system power supply and main switch are positioned at the back side of the instrument.
Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded
circuit capable of delivering the appropriate current and voltage is required for installation.
Electrical requirements is located on the imager label on the rear panel of the imager
Switch on the main switch to position 1 – ON.
If required, switch off the main switch to position 0 – OFF.

The main switch provides power to all the imager components.

The darkroom control board USB cable goes from the darkroom to the computer USB port.

Computer rear side - Connection

Do not connect the camera USB cable and the darkroom camera cable before the software installation

QUANTUM CX5 hardware operating instruction

Warning while using the darkroom

UV WARNING
Switch off the transilluminator when gel is not present on the UV filter. If the filter is too hot,
it will damage your electrophoresis gel.

85
 UV WARNING
Wait for at least 20 second in the "High" position before reducing the intensity selector to
"Low”.

UV WARNING
If one or several tubes are off or used, and in order to keep a better homogeneity, we
recommend to change the 6 tubes simultaneously.

Imager front side – Door open

The QUANTUM CX5 SmartCab intelligent darkroom technology allows:
• Software control of the lighting
• Automatic white light intensity adjustment according to the sample position
• Precalibrated focus for all defined zoom preset

When the door is open, you can access the slide-out transilluminator, the door UV security switch (9) and the on/ off
button (8) of the transilluminator.

8 9

Press twice the transilluminator on/off button (8) to switch on the transilluminator light while the door is opened.
The white light switch on automatically when the door is opened.

86
Optional PadBox and Pad system
The PadBox multimodal container could accommodate our UV, blue, or white light pad or your own hardware such
as heater, cooler, electrophoresis tank, special light source etc. The power socket inside the darkroom is switched on
and off through the software.

The PadBox can easily integrate one of the several available Application Pad.

The PadConnect technology allows an automatic recognition of the installed Application Pad. Imaging and software
options are then adjusted accordingly.

Several Application-Pad can be easily inserted or removed inside the QUANTUM CX5:
• UV-Pad
• Super-Bright-Pad
• Sky-Pad: LED blue light transilluminator, 470nm
• White-Light- Pad: LED white light transilluminator

All Application Pad can be used as a standalone illumination device outside the QUANTUM CX5 system, with an
optional plug adaptor.

87
Filter wheel
The QUANTUM CX5 filter wheel is located inside the darkroom.

The 10 positions filter wheel is supplied with the F590 M58 interferential filter supplied with the system for Ethidium
bromide application. The F590 Ethidium bromide filter is attached to the position 1.
To select the filters, turn the black handle and select the position of your choice.

To install a filter, select the filter position using the handle and screw the filter in its dedicated holder.

88
BIO-PRINT CX4

About the BIO-PRINT CX4 EDGE imaging system

The BIO-PRINT imaging system is a scientific instrument designed to capture fluorescence or colorimetric gel images.
The BIO-PRINT system uses the Bio-Vision software to control image capture and optimization for selected
applications.

A scientific camera is used to capture high resolution digital images of protein and DNA bands in gels obtained by
electrophoresis separation methods. The instrument can capture images of fluorescent, and colorimetric samples,
depending on the system configuration.
The instrument can be used for research purposes in the academia and life sciences industry. The instrument cannot
be used for diagnostic purpose.

BIO-PRINT offers exquisite precision and resolution, which mean reliable results for both quantification and
documentation. The advanced imaging electronics has been developed by our experts especially for your scientific
applications. This association of our exclusive electronic, high-quality optics and advanced software delivers
outstanding performance. With BIO-PRINT, you simply reach the lowest limits of detection on all of your samples.

BIO-PRINT CX4 EDGE system installation

Unpacking the system

WARNING
The BIO-PRINT imaging system is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before installing,
operating or maintaining the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or damage to
the instrument.

Please, open the BIO-PRINT CX4 EDGE box carefully and verify the contents:
BIO-PRINT CX4 1
Power cable 1
USB cable 2
Instruction manual 1
Bio-Vision software CD-Rom inside the instruction manual 1

 Remove carefully each component from the box.

 To remove the BIO-PRINT CX4 EDGE from the box, please use the belts which surround the system and designed
for this purpose
 Do not handle the system by the camera or the camera support.
 Remove their protective plastic cover.

89
 Place the darkroom at its permanent location, the door facing forward. The cabinet has to be placed near the
computer used for image acquisition.

WARNING
It is recommended that the BIO-PRINT CX4 EDGE darkroom be carried by at least two people –
one on each side- holding the instrument from the bottom side. Be sure that the door is
properly closed when carrying the instrument.

The imager weighs 40kg. Using two people, place the imager onto the benchtop given proper
space, electrical and environmental requirements. Remove the outer packaging materials.

WARNING
Do not connect the power cable to a power source until all connections are made. The power
source has to be grounded and protected by a circuit breaker.

WARNING
The BIO-PRINT CX4 EDGE system is designed to fit a specific voltage. Please, check the voltage
to ensure it corresponds to the BIO-PRINT CX4 EDGE specifications.

WARNING
Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air
circulation for the system. The system should be located in an area free of excessive dust or
moisture, strong magnetic fields or ionising radiation. It is also recommended that the
ambient temperature be stable and within the range of 15°C to 25°C (20°C recommended)
and that the relative humidity not exceed 70%, non-condensing.

WARNING
Ensure that all of the systems ventilation openings are free of interference. Excessive heat
build-up in the instrument may affect performance or cause operational failure.

The BIO-PRINT CX4 EDGE system should be located away from water, solvents, or a corrosive
material, on a bench top that is dry and stable. The system should be placed away from
interfering electrical signals and magnetic fields. A dedicated electrical outlet should be used
to eliminate electrical interference from other instrumentation in your laboratory

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 WARNING
Do not defeat any instrument interlocks; they are designed to prevent user injury.

It is compulsory to power down the system and disconnect the AC mains from the unit before
performing any disassembly or repair to the system.

UV WARNING
The use of the BIO-PRINT CX4 EDGE involves ultraviolet (UV) illumination. Proper precautions
must be taken to avoid eye and skin exposure to the UV light. This instrument is meant for use
only by specialised personnel that know the health risks associated with UV radiation and the
chemicals that are normally used with this instrument.

Installing the hardware

Environmental conditions

Placement Place the BIO-PRINT CX4 EDGE on a level-working surface.

The imager requires a stable laboratory bench or table capable of supporting 80 kg.

Provide a minimum clearance of 8cm at the rear of the imager to allow adequate ventilation
for camera cooling and access to the power cord and switch. In addition, allow sufficient
space to open the front-facing imager door to place sample within the imager.

The BIO-PRINT CX4 EDGE is intended for indoor use only.

Other conditions Do not install the equipment near a window and avoid direct sunlight. We recommend
attached blinds to nearby windows

Do not install the instrument where it may get wet or flooded

Do not install the instrument in a dusty environment

Do not install the instrument in a place constantly or excessively exposed to vibration or

impacts (next to a centrifuge or to a compressor for instance)

Remove all hot or heat source near the BIO-PRINT CX4, specifically from the air intake.

Do not place objects near the power outlet to ensure easy access to the power cord for
disconnection in case of emergency.

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 Environmental Temperature: 18°C to 25°C
conditions Humidity: 20% to 70% RH (no dew condensation)

Imager back side

The system power supply and main switch are positioned at the back side of the instrument.
Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded
circuit capable of delivering the appropriate current and voltage is required for installation.
Electrical requirements is located on the imager label on the rear panel of the imager
Switch on the main switch to position 1 – ON.
If required, switch off the main switch to position 0 – OFF.

The main switch provides power to all the imager components.

The darkroom control board USB cable goes from the darkroom to the computer USB port.

Computer rear side - Connection

Do not connect the camera USB cable and the darkroom camera cable before the software installation

BIO-PRINT CX4 EDGE hardware operating instruction

Warning while using the darkroom

UV WARNING
Switch off the transilluminator when gel is not present on the UV filter. If the filter is too hot,
it will damage your electrophoresis gel.

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 UV WARNING
Wait for at least 20 second in the "High" position before reducing the intensity selector to
"Low”.

UV WARNING
If one or several tubes are off or used, and in order to keep a better homogeneity, we
recommend to change the 6 tubes simultaneously.

Imager front side – Door open

The BIO-PRINT CX4 EDGE SmartCab intelligent darkroom technology allows:
• Software control of the lighting
• Automatic white light intensity adjustment according to the sample position
• Precalibrated focus for all defined zoom preset

When the door is open, you can access the transilluminator, the door UV security switch (9) and the on/ off button
(8) of the transilluminator.

8 9

Press twice the transilluminator on/off button (8) to switch on the transilluminator light while the door is opened.
The white light switch on automatically when the door is opened.

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BIO-VISION SOFTWARE – PC VERSION

BIO-VISION software installation

Minimum computer configuration

Minimum requirements

Type Desktop computer format

Processor Intel Core i3 with Intel chipset – i5 recommended

AMD processor and chipset are not supported

Ram 4 Gb and above (8 Gb recommended)

Hard disk 200 Gb and above

At least 10% free disk space least in order to allow software installation and image storage

Monitor/ Video Minimum 1200 columns and 900 lines / 16 million color mode (24-bit).
card Upper resolutions supported. Video card with a refresh rate above 60Hz is recommended.

Operating Microsoft Windows 7 SP1 or Windows 10 SP1 (and upper). 64-bit only
system
At least two USB-3 ports available in the computer rear side for the INFINITY, QUANTUM and
USB Port BIO-PRINT systems

Please make sure to enable the “xHCI” BIOS option to fully activate the USB-3 ports
capabilities

A CD player is necessary to install the software

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How to enable the XHCI Bios option
eXtensible Host Controller Interface (xHCI) is a computer interface specification that defines a register-level
description of a host controller for Universal Serial Bus (USB), which is capable of interfacing with USB 1.x, 2.0, and
3.x compatible devices.
The main xHCI options are as followed:
• XHCI Mode = Disabled - The on-board USB 3.0 port functions like a 2.0 port
• XHCI Mode = Enabled - The on-board USB 3.0 port functions like a 3.0 port
• XHCI Mode = Auto - The on-board USB 3.0 port functions like a 2.0 port before OS USB 3.0 driver load. To
this extend, the Auto mode does not always ensure a proper use the 3.0 port.

You need to select the ENABLED mode to ensure your system is working properly. To proceed, you need to enter
your PC’s Bios settings. Entering BIOS is actually very easy once you determine which key or combination of keys on
your keyboard to press to access BIOS.

1. Restart your computer, or turn it on if it's already off.

Note: Accessing the BIOS is independent of any operating system (Windows 10, Windows 7, Windows whatever) on
your computer because the BIOS is part of your motherboard hardware. Any instructions for entering the BIOS setup
utility will be the same.

2. Watch for a "entering setup" message in the first few seconds after turning on your computer. This message varies
greatly from computer to computer and also includes the key or keys you need to press to enter BIOS.

Here are some common ways you might see this BIOS access message:
o Press [key] to enter setup
o Setup: [key]
o Enter BIOS by pressing [key]
o Press [key] to enter BIOS setup
o Press [key] to access BIOS
o Press [key] to access system configuration

3. Quickly press the key or keys instructed by the previous message to get into BIOS.

Note: You may need to press the BIOS access key several times to enter BIOS. Don't hold the key down or press it too
many times or your system may error or lock up. If that happens, just restart and try again.

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 NOTICE
Windows 7 and Windows 10 offer advanced control over your computer power options. No
BIOS or Windows energy savings should be active when using the instrument.
Please refer to the Trouble shooting section of this User Guide for more information on how
to access advanced Windows power options.

Windows 7 and Windows 10 must be installed in the computer intended to be used to control
the imaging system.

Please run completely the Windows Update before installing the software.

Prerequisite

WARNING
The imaging system should be switched off before the Bio-Vision software installation.
Do not switch on the system before the installation is fully completed.

NOTICE
The CD disk provided with your imaging system contains the Bio-Vision software and driver.

You must have WindowsTM administrator permission to install the software

Bio-Vision software installation

 Insert the CD-ROM in the CD-ROM drive
 Ensure that all other application programs are closed. Windows 7 and Windows 10 users should also ensure that
they are logged on with administrator privileges.
 If the Autorun option is set on your computer, the installation automatically starts. If not, double click on the
Autorun.exe file to start the installation of the software.

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 Please click on the run autorun.exe icon. The Setup window will appear, welcoming you to the Install Wizard for
Bio-Visions setup.

 Please click on the Install software icon.

A pop-up window displays the following Windows installation authorization message:

Click on the “Install” button. The setup wizard will then start to initialize. This could take up to 1 minute:
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The installation software will start to initialize. This could take up to 1 minute:

The setup wizard will then start the installation process:

Step 2.  The welcome screen is displayed, click on NEXT to continue

Click on NEXT to continue

 Select the Destination directory (“c:\Program Files (x86)\Vilber Lourmat\BioVision” by default).

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Then click on NEXT. Program files are installed in the specified directory and group created:

When the software installation is complete, click on Finish. The system will then start the driver installation process:
The setup wizard will then install the USB camera driver.

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Click on Install to install the system’s USB driver.

When finished, click on OK.

Click on OK.

A pop-up window displays the USB controller driver installation window:

Select the “Install” option

 Once the software is installed, the system is updated. This can take a few minutes, depending on the computer
speed. Switch on the imaging system. Keep the Bio-Vision CD-Rom in the CD-Rom drive of your computer.
The darkroom driver is then installed by Windows:

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This driver is related to the imaging system darkroom management.

Wait for 10 seconds and double click on the Bio-Vision icon: .

The camera driver is then installed by Windows.

You can now properly use the imaging system software and hardware.

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Trouble shooting – Windows device manager
In case of installation issue, we recommend to check the driver status using the Windows Device Manager.
To do so, from the Windows start menu, select “Control Panel”.

 The software opens the Windows setting options; For Windows 7 or Windows 10, select System and security, then
system, then Device Manager:

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The Device Manager should appear as below for the INFINITY CX5, QUANTUM CX5 and BIO-PRINT CX4 EDGE when
the systems are properly plugged and powered ON:

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Configure your PC to improve the system performance
This tech note outlines known good practices which are likely to improve camera performance.
Our imaging system is a real-time streaming device which compete with other computer devices for system
resources. The computer power efficiency management makes the CPU constantly changing its speed and
performance. This leads to image artifacts, camera timeouts, and other unwanted imaging errors. In order to
overcome these performance issues, certain settings must be adjusted to ensure the system performance is not
hindered.
The computer Bios and Windows offer advanced control over your computer power options. You can ensure
maximum real-time image streaming performance occurs throughout your image acquisitions, by using the
recommended power settings below.

Bios settings – Disable the CPU EIST function

On Intel Core i3/5/7 computers, the mother board BIOS has enabled CPU speed throttling and power saving by
default (called CPU EIST – Enhanced Intel Speed Test – or “SpeedStep” or “C State”). Depending on CPU loading, Intel
EIST technology can dynamically and effectively lower the CPU voltage and core frequency to decrease average
power consumption and heat production. These settings do not allow for stable image acquisition because the speed
of the CPU is constantly changing.

Follow the suggested settings below to ensure maximum performance. Note: please consult with your
computer/motherboard operating manual or ‘boot screen’ for instructions about how to access settings in the BIOS.

• Disable the Intel CPU EIST (or Intel SpeedStep or CPU C State).
This feature could generally be found in the following Bios menu: Advanced / CPU configuration / CPU Power
management configuration.
Some BIOS menus have grouped these settings into more generally-named options, set these sorts of power savings
options to ‘maximum power’/’least savings’ if there is any doubt.
In some Bios menu, you need to select the “Best performance” option in the advanced CPU configuration.
Please refer to your computer Bios manual for more detailed information.

USB Advanced Power options

Please ensure the camera and the system are connected to the USB rear port of your computer. The front ports are
usually not designed to support device powered by high voltage such as the imaging system darkroom and camera.

Windows tries to determine which USB devices are not actively in use and temporarily suspends them. This can
cause performance degradation for USB cameras and USB storage devices (which a user may use for storing images).
In the advanced power options, find and set:
• USB settings - USB selective suspend setting Setting: Disabled

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To access the power option, open the Windows control panel and select the Power Options

Then select High performance and Change plan settings:

Find and set • USB settings - USB selective suspend setting Setting: Disabled:

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USB Hub Advanced Power options
In the Windows Device Manager, each USB hub need to be set not to turn off.
To access the Windows Device Manager, open the Windows control panel and select the Power Options

Select a USB hub from the Universal Serial Bus controllers menu and right click to open its Properties.

From the Properties window, select Power Management and disable the “Allow the computer to turn off this device
to save power” option:

Repeat for all the USB Hub found in the Universal Serial Bus Controller menu of the Windows Device Manager.

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BIO-VISION - Image Capture Operation
Introduction
This chapter describes how to operate the Bio-Vision software which control the image acquisition from your
INFINITY CX5, QUANTUM CX5 or BIO-PRINT CX4.

Before to operate the imaging system, it is important that you have read and understood the safety information.
Make sure to read this User Guide relevant safety instructions before you start to operate the system.

WARNING
Do not operate your imaging system in any other way than described in the User Manual.

Your imaging instrument is a high-end ultra-sensitive scientific camera platform, designed to extract the lowest level
of detection from your protein or DNA sample. Our superior sensitivity, resolution and dynamics provide optimal
performance across a large array of applications.

The standard imaging process is as follows:

Start the imaging system & software

Position your sample

Capture an image

Visualize your image

Save and print

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Start the imaging system & software

Switch on the system

On the right-hand side of the imager, press the power button to the position I, to turn it on.

After the computer has booted up completely, the following messages are displayed until the system is ready for
image acquisition:

When system is ready, the start menu appears on the display:

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Capture an image
This chapter describes the steps to acquire an image.

Fluorescence image acquisition

Fluorescence is the main method used for gene expression and protein detection. It results from a process that
occurs in molecules known as fluorophores. The fluorophore absorbs the excitation light, reaching a higher energy
state. By returning to its former state, it emits fluorescent light.
The Imaging system separates the emitted light from the excitation light in order to obtain an optimum sample
image. According to the configuration, the Imaging system can accommodate several excitation and emission
channels in the visible, RGB and UV area and is ideal for a large array of applications such as 1D DNA gel, 1D protein
colorimetric samples, multiplexing, stain free gel and blot.

Image capture workflow

The basic steps to acquire an image are:

Choose the application type

Acquire the image

Save or print the image

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Choose the application type

The Application selector

The imaging system runs with pre-defined image acquisition parameters to facilitate the use of the system for a
specific application. These pre-defined parameters are gathered in the imaging system Application Protocol and can
be selected from the Application Selector:

The imaging system Application protocol will ease the imaging workflow by automatizing the exposure parameters
adjustment. The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular
basis. It stores the following information:
• The image exposure pre-defined set-up;
• The live preview pre-defined parameters;
• The image display pre-defined set-up;
• The image printing pre-defined set-up;
• The image file pre-defined set-up.

The benefits of the Application protocol are as follows:

• Time saving
• Reproduction of image acquisition parameters
• Protocols are modifiable, allowing the user to maintain an original template while modifying it for a slightly
different result, with minimal effort

The factory settings include several pre-defined Application protocols. You can also create your own Application
protocol. Please refer to the Application protocol chapter of this User Guide to have more details on the way you can
create your own Application.
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For a UV excited gel sample, select the UV fluorescence application from the Application Selector window:

For a colorimetric sample using white light conversion screen, select the Conversion screen application from the
Application Selector window:

For a colorimetric sample using epi white light (light from the top), select the White light application from the
Application Selector window:

Acquire the image

The imaging system has three exposure mode:
• The live mode
• The auto mode
• The start mode

The exposure modes are gathered in the exposure mode menu:

The Live mode

Live mode allows direct visualization of the image. This mode enables you to adjust the focus, and to position your
sample.

Note: A live image means the image displayed is refreshed every 1/20th of a second. This short exposure time (or
frame) is adequate for a variety of white light samples including protein gels and autoradiography. A live image,
however, is not sufficient for most samples, which are visualized and photographed over a relatively dim UV light
source. A feature called integration compensates the low light situation by allowing the camera to obtain a timed
exposure.

Note: After 2 minutes, the software will automatically stop the live preview.

Note: The Preview mode is designed to help for sample positioning and focusing. To this extend, the Preview mode
is always with white light EPI illumination.

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Note: The default Preview mode exposure time is 40 milli-second. For very bright sample, that could be reduced to
20ms. Exposure time could also be increased for dim sample.

The selection of the Live mode will open the following menu:

Adjust the zoom and focus

Modify the zoom and focus settings.

The Zoom in allows you to change the size of your sample, on the image. To proceed, click on arrow buttons. The
zoom automatically moves to the new value. The zoom control will modify the focus accordingly to keep a sharp
image.

The Focus is needed to adjust the sharpness of the image by changing the focal point of the lens. To proceed, click on
the arrow buttons.
You can reset the values to the one saved in the user profile. You can also save the new values in your user profile.

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Display the saturation
Click on “Saturation” to displays the saturated pixels in red.

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.
A 16-bit image has a maximum grey level of 65535

Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image

Note: A saturated image creates quantification error when studied by an image analysis software. Decrease the
exposure time to avoid saturation.

Case 1: Case 2:

Original display Same image with the saturated pixels

indicated in red

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Display the 3D image
The 3D Scan images your sample in real time and reconstructs the data to create live three-dimensional model.

The 3D reconstruction provides direct information regarding the image dynamic, background level and protein or
DNA quantity. A little change of exposure time will refresh the 3D view automatically. The saturation effect can be
controlled live before the image is frozen.

Display the inversed image

Click on the “Inverse” to inverse the grey level of the image. This makes a negative image.

Case 1: Case 2:

Original display Inverted image - Negative image

(black spot, light background)

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Click on Stop to finalize the image acquisition process:

The Auto mode

The imaging system calculates automatically the optimum exposure time (Auto-exposure). When you select the
Auto-Exposure option, the system samples the light levels and uses the values to calculate the final exposure time.
To set the auto-exposure mode, select Auto from the exposure mode menu:

The Start mode

The imaging system requires the user to specify the exposure time.
To set the exposure time, select Manual from the exposure mode menu:

The selection of the Start mode will open the following menu:

Enter the duration of the exposure.

Define your exposure time using the arrow buttons:

Note: The integration time increase or decrease by 40 milli-seconds except for 10 and 20ms.
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Note: With long integration time, a delay could be necessary before an image is displayed on the monitor (up to
twice the selected Exposure time).

Note: When the specified exposure time is reached, the last captured image is displayed. The camera continues to
integrate the image on the sensor, updating the display whenever the specified Exposure time is reached. The Stop
exposure button stops the exposure process. The last full exposure is displayed.

You can also modify the zoom and focus settings, display the 3D image and the saturation. More details are provided
in the Live mode chapter.

The image dynamic and the exposure time is displayed in the control bar on top of your sample image:

The image dynamic is crucial for quantitative accuracy. It should to be the largest possible within the gray scale of
the system.
 65 535 gray levels for 16-bit imaging

The gray scale is the total number of levels between white and black. The image dynamic range within this scale.
Adjustments are necessary to obtain an image with the broadest dynamic. With most of the gels, a real-time image
has a limited dynamic. In this case, the exposure time enhances the image dynamic.

In order to obtain an optimum image, you can control the image dynamic by adjusting the exposure time.

Click on Stop to finalize the image acquisition process:

Save or print the image

Clicking on Save will start the saving of the captured image.

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Note: The image file is named by the system. The default image name has 8 characters. The first 2 are IM. The last 6
are a number ascending from 000001 to 999999.
Note: The default file name is incremented by one each time an image is saved.
Note: The file extension varies according to the image file format:
 For TIFF, the extension is TIF
 For BMP file, the extension is BMP;
 For JPEG, the extension is JPG

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To print, press the “Print” key:

The image is printed on the default printer, if defined.

Note: a printer message can appear on the display in the case of lack of ink or paper. In such case the message
should disappear after few seconds. If not, please press any touch from the panel and the message will disappear
after few seconds.

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BIO-VISION - The Display Menu
The Display menu appears after an image acquisition is stopped

Adjust the image display range

The Display option menu is helpful to enhance the image display by modifying the image greyscale selection to be
displayed. In standard, the Imaging system images have 16-bit format and Windows® can only display 8-bit images
(256 grey levels). Due to this limitation, the software handles two images:
• A “memory” image corresponding to the 16-bit format (65 536 grey levels)
• A “displayed image” corresponding to the image displayed on the screen (256 grey levels)

The easiest way to calculate the “display image” would be to translate the full grey scale each time an image is
acquired: the x grey levels values of the “memory” image corresponds to 256 values in the displayed image. In that
case, it won’t be possible to visualize faint spots on a dark image.

The software offers the possibility to select the grey level range to translate for the display image calculation. All the
grey levels under the “Min value” defined will be converted to 0 (Black) in the displayed image. All the grey levels
upper the “Max Value” defined will be set to 255 (White) in the displayed image. The grey levels between those two
limits will be converted in an intermediate grey level value following a linear rule.

You can select one from the 5 image display presets:

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The image displays presets goes from the minimum image grey levels to 20%, 40%, 60%, 80% or 100% of the image
depth.

Case 1: Case 2:

Default optimum display Optimum display enhancement

The image appears brighter. The faint
bands are more visible.

Note: The Display function inform you of the minimum and of the maximum grey level obtained on the image.

Note: The Display has no impact on the analysis. Only the display of the image is modified.

Display the saturation

Click on “Saturation” to displays the saturated pixels in red.

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.
A 16-bit image has a maximum grey level of 65535

120
Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image

Note: A saturated image creates quantification error when studied by an image analysis software. Decrease the
exposure time to avoid saturation.

Case 1: Case 2:

Original display Same image with the saturated pixels

indicated in red

Display the 3D image

The 3D Scan images your sample in real time and reconstructs the data to create live three-dimensional model.

The 3D reconstruction provides direct information regarding the image dynamic, background level and protein or
DNA quantity. A little change of exposure time will refresh the 3D view automatically. The saturation effect can be
controlled live before the image is frozen.

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Display the inversed image
Click on the “Inverse” to inverse the grey level of the image. This makes a negative image.

Case 1: Case 2:

Original display Inverted image - Negative image

(black spot, light background)

Autofit or zoom in / out on the image

• Autofit
Click on the “Autofit” to resize the image to fit the size of the monitor.
The full resolution of the acquired may be larger than the screen resolution. The navigation requires the Windows
scroll bar. The Autofit allows you to view the whole image, regardless of the window size. Typically, reducing the size
of a window also cuts off part of the image. The Fit to Window option solves this problem by resizing the image so
that it is always the same size as the window.
The Autofit proportions the display of the image to the screen resolution.

• Zoom
Click on the arrows to zoom in or out the image

Case 1: Case 2:

100% 150%

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Access the Good Laboratory Practice (GLP) file
The Good Laboratory Practice (GLP) file is made to track all the image treatments performed with the software. Click
on the “GLP” icon. A pop-up window displays the following menu:

The GLP file has 4 different topics:

- the Image details which provide information on the way the image has been taken;
- the Application protocol which provide information on the Application protocol used to take the image;
- the System details which provide details on the system used to take the image;
- the Notes which provide you space to add information to your image.

Display the image using pseudo colors

The pseudo colors can display different types or levels of fluorescence in an image. It replaces the original grey levels
of the image by another palette color. The software has several predefined palette designs. Select your palette
design from the followings:

123
Original image GFP painted image Coomassie painted Multicolored Red painted image
image painted image

124
BIO-VISION - The Edit Menu

Text
Click on the “Text” from the Edit menu. A window displays the following menu:

You can annotate your image with text and arrows.

•Text
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Left click at the point where you want to insert the text. The Text window appears. Enter the text.
You can define the appearance of the text. Select the Modify font to modify the font, the font style and the text size.

You can modify the font color by selecting the color option:

You can select the text orientation by clicking on the corresponding button:

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•Object insertion

You can use an arrow or a rectangle to emphasize a particular area in an image. To proceed, click on the following
icon:

to draw an arrow
Note: You can turn the arrow direction by using the right click of your mouse.

to draw a rectangle

You can modify the object color by selecting the color option:

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You can insert symbol by clicking on the Symbol button. You can also add the following items to the image:
- Date. Add the current date or the GLP date to the image. The current date is the date set on the computer you are
using. The GLP date is the image acquisition date as recorded in the Good Laboratory Practice file (GLP).

- Time. Add the current time or the GLP time to the image. This current time is the time set on the computer you are
using. The GLP time is the image acquisition date as recorded in the Good Laboratory Practice file (GLP).

- Image name. Add the image title to the image. The full image name is the file name and location of the opened
image. The short image name is the file name of the opened image.

- Symbol. You can select a symbol from the symbol fonts and insert it on your image

• Text or objects position

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Select the objects that you want to align:

Use the “Select objects” button to select multiple objects of your choice and drag a dotted box over the objects
using the on the image
Use the Select all objects” button to selects all the text objects from your document

Note: the selected objects are surrounded by an orange frame:

Note: the master object is surrounded by a bold orange frame:

Then align the text objects according to your desired layout:

Align left
Align right
Align top
Align bottom

Arrange horizontally objects equal distances from each other . Select at least three text objects you want to
arrange and then click on Distribute Horizontally

Arrange horizontally objects equal distances from each other . Select at least three text objects you want to
arrange and then click on Distribute Vertically

Note: text objects are aligned or arranged according to the master object position.

Note: you can delete N object by selecting the object and clicking on the DEL key

• Template
The text can be saved as a template and re-used for further analysis to facilitate routine text addition.
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The template automates a task or set of tasks that you perform repeatedly or on a regular basis. It stores all the text
comments. You can save the template created on one image and / or load the template on another image.

The benefits of the template file are as follows:

- Time saving
- Reproduction of image analysis parameters
- Templates are modifiable, allowing the user to maintain an original template while modifying it for a slightly
different result, with minimal effort

Crop the image

You can cut out a portion of an image. Click on “Crop” from the Edit menu. A window displays the following menu:

A default area is displayed. Click on the tags surrounding the area to modify its size. Drag and drop the area to
modify its position.

Note: To delete a previously defined area, click once again on the function.

Click on the “OK” to crop the image:

Note: You can undo the cropping by clicking on “Undo”

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Note: Exit the Cropping function by clicking on “Ok” to validate the selection or “Cancel” to discard it and return to
the original image.
Note: the defined area can be moved with the keyboard arrows.

Rotation

You can rotate your image clockwise in 90° increments or mirror it vertically or horizontally. Click on “Rotation” from
the Edit menu. A window displays the following menu:

Click on the “Rotate” icon to rotate right the image. The image is rotated clockwise in 90° increments:

Before

After

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Click on the “Vertical mirror” icon to flip the image from top to bottom.

Before After

Click on the “HorizontalVertical mirror” icon to flip the image from right to left.

Before After

The “Rotate by angle” icon to rotate the image in increments other than 90°. Click on the rotation icon. A window
display the following menu:

You can also define the angle of rotation in degrees. To complete the rotation, click on the Rotate button. Click on
the Undo button to come back as previously.

Thanks to the axis, which appears on the image, you can also directly rotate the image using this overlay. To perform
the rotation, position the cursor on the green square and drag in the green arrow direction. As you drag, the arrow
will rotate and the angle in the box will change. Adjust the green lane to be as parallel as possible as the lanes. To
complete the rotation, click on the Rotate button.

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Adjust the green line to be as parallel as possible from the lane. Then, click on OK.

Background subtraction

The Subtract background feature allows to separates foreground objects from their background clutter. Image
background could interfere with quantification or signal visualization. Background could result from gel opacity,
random signal noise, opacity of the carrier medium (film, gel matrix, or blot matrix) or film fogging.

The Subtract background applies to the active image. Click on the “Subtract background” button. A window displays
the following menu:

Select the removal intensity and click on apply. The background is then removed from the entire image.

The intensity will determine how much background will be subtracted. A large intensity will more closely follow the
profile trace, removing more background. A too small intensity will result in poor Subtract background. A disk radius
that is too small may subtract actual data.

Note: few seconds could be necessary to perform the background subtraction.

Note: For a more accurate background subtraction, you can set the bands parameters: white bands / black
background / black bands, white background. You can select the following options:

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 White bands on dark background for images with white signals
and black background

Black bands on light background for images with black signals

and clear background

Example:

Original image Image with Subtract

background

Flat field

The Flat field feature correct for uneven illumination intensity for both the background and the signal.
Even under the best of imaging conditions, the illumination across a field of view isn’t perfectly uniform. This is due
to the light source imperfections (i.e. slight misalignments and additional physical properties) and to the optical
elements constraints (bulb, filters, mirrors, objectives) within a light path.
Flat Field Correction is a commonly used approach for this problem. This process will compensate for different
illumination intensity. In the flat fielded image, a uniform signal will create a fairly uniform output (hence flat-field).

The Flat field applies to the active image. Click on the “Flat field” button. A window displays the following menu:

Click on OK to get the flat fielded image.

Note: few seconds could be necessary to perform the flat fielding.

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Note: For a more accurate background subtraction, you can set the bands parameters: white bands / black
background / black bands, white background. You can select the following options:

White bands on dark background for images with white signals

and black background

Black bands on light background for images with black signals

and clear background

Artifact correction

The Remove artifacts function removes dust or small defect from an image

This function replaces a pixel by the median of the pixels in the surrounding. The Remove artifacts applies to the
active image. Click on the “Artifact correction” button. A window displays the following menu:

The radius defines the size of the artifacts to be removed. This value is given in pixel.
The threshold determines by how much the artifact pixel must deviate from the median to get replaced. This value is
given in grey levels.
The type of artifact determines whether the brighter pixels or the darker pixels than the surrounding should be
replaced.

Click on OK to remove the artifacts from the entire image.

Multiplexing

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The multiplexing function allows combining two or three different fluorescent channels into a new colored
composite image. The multiplexing option is used primarily in fluorescence imaging when specimens have been
stained with more than one dye.

Click on the “Multiplexing image” button. A window displays the following menu:

A list displays the image already opened. You can also add an image to the list by opening an image from your
computer. To proceed, click on the “From disk” button. A pop-up window displays the following menu:

Browse to specify the image directory

Double click on the image name you want to load

When all the multiplexing images are gathered in the list, click on the first image. A pop-up window invites you to
select the first color channel from blue, green, red:

When the first channel is selected, proceed in the same way for the one or two others channels. You will obtain an
image list codified by the color channel.

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Click on the “OK” button. A window displays the following menu:

A new color composite image appears in the main windows, gathering the multiplexing images, channel by channel.
Example of a composite image elaborates from 3 different channels:

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You can adjust the displays of the composite image. To proceed, click on the thumbnails you want to modify. The
Display Adjustment graph for the channel you selected will appear in the menu:

Use the Display Adjustments to optimize the display to enhance the features of interest in the image.
Adjust the display by moving the slider, or by typing a number in the value box.

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BIO-VISION - The Application Protocol
What is an Application protocol?
The software runs with pre-defined image acquisition parameters to facilitate the use of the system for a specific
application. These pre-defined parameters are gathered in the software Application protocol.

The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular basis. It
stores the following information:
- The image exposure pre-defined set-up;
- The live preview pre-defined parameters;
- The image display pre-defined set-up;
- The image printing pre-defined set-up;
- The image file pre-defined set-up.

The factory settings include several pre-defined Application protocols

You can also create your own Application protocol.

The benefits of the Application protocol are as follows:

- Time saving
- Reproduction of image acquisition parameters
- Protocols are modifiable, allowing the user to maintain an original template while modifying it for a slightly
different result, with minimal effort

How to access the application protocol?

Select an application protocol from the application menu. Click on the application name to open the application
protocol menu.

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The Application protocol can either be displayed with a summary view or with a complete view. By default, the
application protocol is displayed with the summary view. Click on Advanced options to have access to all the
application protocol features.

Advanced exposure – Exposure predefined set-up

Exposure folder contains the exposure pre-defined set-up:

Typical Exposure set-up for a fluorescence application

• Zoom options
The standard mode allows you to use the E-Box in a portrait mode.

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You can select till 6 zoom preset positions. Position 1 correspond to a field of view of roughly 27x20.

Zoom options are as followed:

Approximate field of view according to the zoom mode:

Zoom options
1 260x200mm
2 200x170mm
3 170x130mm
4 140x110mm
5 100x80mm
6 80x55mm

• Lighting (Fluorescence mode only)

This option defines the lighting used for the image exposure. All systems are equipped with LED Visible light. You can
select between low and high intensity.

Note: The lighting options depend on your system configuration. UV, or blue lighting could be available. The
selection of these lighting options will be done from this menu.

• Extended resolution
The effective pixels technology allows the extension of the camera native image resolution by software
interpolation.

Note: This process requires important computer calculation and sometime could be necessary before the extended
image is displayed.

• Auto-exposure set-up

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Test time
In order to calculate an ideal exposure time, the software needs to analyze a test image, taken in binning mode with
a test exposure time. Then it searches for the minimum and maximum acceptable grey levels and calculate the
exposure time so the highest grey levels values of the final image reach a maximum defined (Saturation Value).

Note: in the case the auto-exposure does not give you entire satisfaction for your specific application, we
recommend increasing the test time.

Auto mode
The Auto mode options allow you to select between a standard auto exposure time, an extended auto exposure
time or a reduced auto-exposure time.

Standard exposure is optimized for all bands.

Over exposure (faint band) results in a longer exposure where faint bands are more visible, but more prominent
bands may be over exposed.
Under exposure (bright band) results in a shorter exposure where bright bands are more visible, but more prominent
bands may be under exposed.
User defined allows you to decide of the over exposure or under exposure settings. For instance, 150% will increase
the standard exposure time by 1.5.

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• Manual exposure set-up

Manual exposure set-up

This option defines the default imaging exposure time. The software has two exposure time scales:
- One for short times: 40 milli-second to 4 seconds
- One for long times: 4 sec to 1 minute

Note: With the short integration time scale, the integration time increases or decreases by 40milli seconds.
Note: With long integration time, a delay could be necessary before an image is displayed on the monitor (up to
twice the selected Exposure time).

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Preview – Live preview pre-defined parameters
Preview folder contains the live review pre-defined set-up:

• Extended resolution
The effective pixels technology allows the extension of the camera native image resolution by software
interpolation.

Note: This process requires important computer calculation and sometime could be necessary before the extended
image is displayed.

• The lens prefixed position table

The lens prefixed position table summarizes the default focusing preset per zoom option for the concerned
Application protocol.

Note: The default positions are part of the system factory settings. Nevertheless, you can save your owned position
using the zoom menu you can access during image acquisition.

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Display – Image display pre-defined parameters
The Display folder contains the image display pre-defined set-up:

• Select display mode

The Select display mode options allow you to select between a standard display, an enhance display for the faint
band and an enhance display for the bright band.

Standard display is optimized for all type of image.

Enhance display for faint band ensure the faint bands are more visible, but more prominent bands will look very
strong.

Enhance display for bright band ensure the bright bands are more visible compared to their background. This is well
adapted for fluorescence sample.

• Set the gamma value

Gamma adjustment corrects an image by creating a new version of the original. To create the new image, the
Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the following
graph:

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The above graph demonstrates the basic principles of gamma adjustment:
- Black (pixel value = 0) remains black at all gamma values.
- White (pixel value = full scale) remains white at all gamma values.
- Gamma values greater than one lift the darker areas of the original image into the brighter areas of the new image.

A gamma curve is smooth: there are no unexpected jumps or cut-offs. This means that when viewing a gamma
adjusted image, you will be able to see the details (intensity differences) in both the black and white areas of the
image.

When the bright areas of these types of images are correctly exposed, the darker areas can be so dark that they are
in effect invisible. Gamma Adjust can remedy this problem. The gamma adjustment results in a better display of
detail by lightening the darker areas without burning out bright areas or lightening black areas:

• Inverse
Select “Inverse” to inverse the grey level of the image. This makes a negative image.

• Display the saturation

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.

The maximum grey level depends of the bit-depth. For instance:

A 16-bit image has a maximum grey level of 65535

Select the saturation option and the saturated pixels are displayed in red:

Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image

Note: A saturated image creates quantification error when studied by an image analysis software. Gel-doc systems
have to indicate to the user if the image is saturated and if it is then necessary to modify the integration time.
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• Select the color palette
The pseudo colors can display different types or levels of fluorescence in an image. It replaces the original grey levels
of the image by another palette color. The software has several predefined palette designs. Select your palette
design from the followings:

Printing – Image printing pre-defined parameters

The Printing folder contains the image printing pre-defined set-up:

• Print with image name

Print the image with its name.

• Print with date and time

Print the image with the computer time.

• Print out text color

Select the color of the text printing.

• Free text print-out

You can select a free text (for instance the name of your institute), the image name (if the image has been previously
saved) and date and time.
The text is then displayed on the image print out:

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General – Image file pre-defined parameters
The General folder contains the image file pre-defined set-up

•User name
You can define the name of a user for an application protocol. The user could be the name of a working group, the
name of a user or a subtitle to the name of the application.

•Protocol’s avatar
To simplify the reconnaissance of the Application’s protocol, an icon is provided to differentiate the application by a
color.

•Default file name / Image name prefix

The default file name could either be based on the Date and Hour the image has been taken or by a prefix. You can
decide of the default generic file name to simplify the file name generation. By default, the name is IM00000x. The
image could also be named according to the time and hour it has been taken.

•File format
Images can be saved in CompatibilityPlus TIFF scientific image format (16-bit format recommended), TIFF scientific
image format (16-bit format), BMP (8-bit format only), JPEG (compressed) or APX (proprietary file format – 16-bit
format). This option allows you to select the default file format.

•Preferred image saving directory

This option defines the directory where the image is saved by default
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Alternatively, you can select to always open the last used directory by clicking on the following option:

•Autosave
Use the Image Auto-Save feature to automatically save images as part of the capture process, in the defined image
saving directory.

•Preferred settings saving directory

This option defines the directory where are saved the configuration files.

Select to automatically activate the post processing after the image acquisition.

Note: Please refer to the Processing menu to have more details on the post process working principles.

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BIO-VISION - The System Settings
Settings feature allows the adjustment of the system settings such as the filter controls or the lens calibration. Click
on “Settings” from the menu barre:

A window displays the following menu:

The Settings menu contains the following features, related to the system:

General Displays the system information such as the system option, the software
version, the filters installed and the lens calibration state.

Language selection Select the language you want the software to run if the language pack is
installed on your system

CFR21 mode Set the CFR21 mode

The general system settings

The General header from the Settings menu summarizes the information concerning your system and its set-up. It
gives you access to the following information:
 Systems name & version
 Camera and darkroom state
 Options installed
 Calibration stat

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To proceed, select the General header from the Settings menu.

Language selection
The software could run in English, in German, and in Chinese if the language pack is installed on your system. Select
your language from the list. To proceed, select the Language selection header from the Settings menu:

Then select the appropriate language from the pop-up windows:

You will be prompt to restart the software for the change to occur.

To close the Settings menu, click on or

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CFR21 mode activation

The 21-CFR mode is a closed system which ensures data security. These features are provided to ensure protection
of records:
• The image data is saved in a document which contains all the actions taken to results in this image
• The image document is saved is a proprietary file format which can be open only with the Vilber’s software set
in the 21-CFR mode
• The data are saved with the user security of the signed-in user
• Records created on a 21 CFR 11 system cannot be opened on a non-21 CFR 11 system

To activate the 21-CFR mode, you will need the CFR21-Administrator software. Please contact your local dealer for
more information.

From the software, select the CFR21 mode header from the Settings menu and click on Activate the CFR21 mode:

To close the Settings menu, click on or

Export system
You can export in a text file the system details. This could be necessary for service investigation.

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Run TeamViewer for premium service support

TeamViewer is a proprietary computer software for remote control and file transfer between computers.
For customer support, under your instruction, TeamViewer generates a session password (one-time password). If
your tells us your password, we can connect to your computer with your invitation.
No one can access your computer without your invitation and without knowing the session password.

Note: Your computer need to be connected to the network.

Click on Run TeamViewer.

The TeamViewer window will open.

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Please send us the TeamViewer ID and password for remote access to your computer.

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BIO-VISION - The Analysis Menu
Access the analysis software
Access the analysis menu by clicking on the Analyse button:

Select the Molecular weight icon to open the molecular weight analysis (MW) module
Select the Optical density - 1D icon to open the optical density (OD) analysis module based on a 1D detection
Select the Bio-1D menu to open the image in the optional Bio-1D software.

Analysis software interface

The analysis module opens on the following window:

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The analysis software operating environment is organized into three areas:

The dash board is different for each analysis module. For molecular weight, it contains the following tabs:

1. Detect
2. Analyse molecular weight (MW)
3. Home

For the Quantification analysis module, the dash board contains three different tabs:

1. Window definition
2. Spot quantification
3. Home

The image window displays the active image. It also contains the image toolbar:

Print the image

Set the band parameters (white bands / black background ;

black bands/ white background)
Copy to clipboard

Display the molecular weight on the image

Change the image display parameters

Autoscale

Zoom in or out the image

Export the table to Microsoft Excel

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Print
The Print function prints a previously opened image as it appears in the image window.

Click on the “Print” icon. A pop-up window displays the following menu:

Click on Print to print as previewed.

Choose a printer
Click on Properties to modify the default setting of the printer, if necessary.
Select the number of copies.
Click on OK to validate your options.

Note: After you have installed and setup your printer, the procedure for setting up and configuring a printer is the
same as in other Windows program.

Black / White bands

This function set the bands parameters (white bands / black background / black bands, white background).
To proceed, click on the Black/White bands icon. A pop-up window displays the following menu:

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You can select the following options:

Automatic detection. Capt software will automatically detect

the bands color scheme according to the defined percentage
of tolerance

White bands on dark background for images with white

signals and black background

Black bands on light background for images with black

signals and clear background

Copy to clipboard
The Copy to clipboard function copies an image, a table or a graph onto the clipboard for insertion into another
program. This option is identical to the Windows® [Ctrl C] command.

To proceed, click on the Copy to clipboard icon. The image, the table or the graph is now ready to be pasted into
another application.

Open the application that you want to paste the image into, and select from the available pasting options ([Ctrl V]
command for Windows® software).

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Display the molecular weight
Click on the “Display the MW” to display the calculated molecular weight on top of the image.

Default display Molecular weight displayed on the image

Image display parameter

Click on the “Image display” to displays the Image Master data as well as the Image Display set-up.

The Image Master data refers to the dynamic range of grey levels in between the minimum and the maximum pixel
intensities obtained in an image.

Image depth is expressed as graduation level. In an image, the density range between white and black is divided into
a number of gradation levels. For instance, a 16-bit image has 65.384 gradation levels. The image dynamic refers to
the number of grey levels in between the minimum levels obtained and the maximum level obtained on a specific
image.

The Image Master data informs you of the obtained dynamic on your image compared to the potential image depth.

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Access the Image Display from the main software windows:

The optimum display window is helpful to enhance the image display by modifying the image greyscale selection to
be displayed.

In standard, the images have 16-bit format and Windows® can only display 8-bit images (256 grey levels). Due to this
limitation, the software handles two images:
A “memory” image corresponding to the 16-bit format (65 536 grey levels)
A “displayed image” corresponding to the image displayed on the screen (256 grey levels)

The easiest way to calculate the “Default” would be to translate the full grey scale each time an image is acquired:
the x grey levels values of the “memory” image corresponds to 256 values in the displayed image. In that case, it
won’t be possible to visualize faint spots on a dark image.

The software offers the possibility to select the grey level range to translate for the display image calculation. All the
grey levels under the “Min value” defined will be converted to 0 (Black) in the displayed image. All the grey levels
upper the “Max Value” defined will be set to 255 (White) in the displayed image. The grey levels between those two
limits will be converted in an intermediate grey level value following a linear rule.

For both values, you can:

• Edit the value in the corresponding field
• Select the value by dragging and dropping the arrow
• Click on the “optimum display” button: the software will then calculate the ideal values to be selected according to
the parameters defined

Note: The optimum display has no impact on the analysis. Only the display of the image is modified.

Case 1: Case 2:

Default optimum display Optimum display enhancement

The image appears brighter. The faint
bands are more visible.

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Click on “Default” to calculate the ideal pixels values to be displayed according to the defined parameters in the
application protocol.

Note: Click on Default to reset the display to the default display settings:

Click on “Inverse” to inverse the grey level of the image. This makes a negative image.

Case 1: Case 2:

Positive image Inverted image - Negative image

(white spot, black background) (black spot, light background)

Click on “Saturation” to displays the saturated pixels in red.

A saturated image is inappropriate for image quantification with image analysis software. The saturation option
allows you to visualize in red, pixels that have the maximum grey level in order to avoid flattened peaks.
The maximum grey level depends of the bit-depth. For instance:
A 16-bit image has a maximum grey level of 65535
A 8-bit image has a maximum grey level of 255.

Note: If an image is being acquired and the «Saturation» option is checked, the modification is applied to the current
acquired image

Note: A saturated image creates quantification error when studied by an image analysis software. Decrease the
exposure time to avoid saturation.

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 Case 1: Case 2:

Original display Same image with the saturated pixels

indicated in red

• Gamma

Gamma adjustment corrects an image display by creating a new version of the original. To create the new displays
range, the Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the
following graph:

The above graph demonstrates the basic principles of gamma adjustment:

- Black (pixel value = 0) remains black at all gamma values.
- White (pixel value = full scale) remains white at all gamma values.
- Gamma values greater than one lift the darker areas of the original image into the brighter areas of the new image.

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A gamma curve is smooth: there are no unexpected jumps or cut-offs. This means that when viewing a gamma
adjusted image, you will be able to see the details (intensity differences) in both the black and white areas of the
image.

When the bright areas of these types of images are correctly exposed, the darker areas can be so dark that they are
in effect invisible. Gamma Adjust can remedy this problem. The gamma adjustment results in a better display of
detail by lightening the darker areas without burning out bright areas or lightening black areas:

• Pseudo colors
The pseudo colors can display different types or levels of fluorescence in an image. It replaces the original grey levels
of the image by another palette color. The software has several predefined palette designs. Select your palette
design from the followings:

Original image GFP painted image Coomassie painted Multicolored Red painted image
image painted image

• Autofit
Click on the “Autofit” to resize the image to fit the size of the monitor.
The full resolution of the acquired may be larger than the screen resolution. The navigation requires the Windows
scroll bar. The Autofit allows you to view the whole image, regardless of the window size. Typically, reducing the size
of a window also cuts off part of the image. The Fit to Window option solves this problem by resizing the image so
that it is always the same size as the window.
The Autofit proportions the display of the image to the screen resolution.

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• Zoom
Click on the arrows to zoom in or out the image

Case 1: Case 2:

100% 150%

• Excel
This function transfers the results table to Microsoft ExcelTM.
To proceed, click on the “Excel”TM icon. The Excel software is automatically opened by the software and the table is
transferred to ExcelTM.

In order to authorize Excel to open the file, please follow the procedure below:
1. Open up Excel 2016
2. Click on File > Options > Trust Center > Settings > File Block Settings

3. Untick all
4. Select “open selected files in Protected View and allow editing”
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5. Click OK on both windows and restart Excel.

Molecular weight analysis module

The software Molecular Weight module features the calculation of electrophoretic distances according to markers or
standards:
in molecular weight (unit: KiloDalton)
in fragment sizes (unit: Kilobases)

A regression method is used to calculate the molecular weight/size of the unknown bands. The software will use the
molecular weight values of the marker (standard) bands to calculate the standard curve. This standard curve is then
used to calculate the values of the unknown bands.

At the end of the process, you can have the following outputs:
- Molecular weight marker’s profile and migration curve
- Molecular weight values

Detect – Edit lane

The molecular weight module opens on the Edit lane dashboard of the Detect process:

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The dashboard details the lane edition parameters:

• Area of interest
On the image, click and drag to define the analysis area and to overlap the lanes. You can easily adjust the size of the
area by clicking on the tags surrounding the area and drag the selected border to the requested size.

The lanes are defined by green lines, overlaid on the gel image. The gel area is surrounded by square anchors:

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To resize the entire lane frame, drag an anchor point in or out. The opposite anchor point will remain fixed while the
frame expands or contracts. The frame will expand or contract from the center.

To move the entire frame to a new position, position the mouse on the frame to obtain a cross cursor:

Click and drag the cursor to move the entire frame.

Note: it is not necessary to include the well line in the area of interest window. The calculation of molecular weight
does not require this reference line.

• Lane direction
Select the direction of the lanes from horizontal or vertical

The lane direction is automatically modified on the image.

• Number of lanes
Select the number of lanes:

The number of lane is automatically modified on the image.

• Gap between the lanes

Define the gap between the lanes:

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Short gap Large gap

The gap between the lanes is automatically modified on the image.

The “Reset” button restores the default lane detection parameters.

• Next
The “Next” button validates your parameter and opens the following analysis step.
A- Edit lane B – Edit band

• Help menu
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Detect – Edit bands

The Edit band process follows the lane definition:

The edit band process automatically identifies all the bands for the defined lanes. You can also manually mark the
bands on the image or on the lane’s profile. All bands will be automatically detected when you first access the band
detection process, based on default parameters.

The bands are marked by green lines, overlaid on the gel image.

Note: you can either access the lane definition menu by clicking on the next button of the lane definition or directly
on the band detection tab.

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The dashboard details the edit band parameters:

• Band detection on the image

You can add or remove bands by clicking directly on the image. Place the cursor at the chosen location and click. The
band is immediately added or removed. The red line allows you to check band alignment between lanes.

• Band detection on the profile

The profile is calculated based on the average intensity of each row of pixels across the specified width of the lane. A
lane profile provides a visualization of the intensity of the bands. Bands are represented by peaks.
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You can add or remove bands by clicking directly on the profile. To proceed, select the cursor type:

The linear cursor has the shape of an arrow ( )

The rectangular cursor has the shape of a square ( )
Place the cursor at the chosen profile location and click. The detection line is automatically added or removed.

Note: For arrow cursor, the band is added at the cursor position.
For rectangular cursor, the band is added at the highest position within cursor bounds.

• Profile visualization

In the profile parameter window, you can select the lanes’ profile to be displayed. To do so, just click on the profile
name to select or unselect the profiles.

You can reset the detection by clicking on the “Reset” button. The detection lines will then be removed.

You can re-detect the bands by clicking the “Re-detect” button. The detection is based on the default parameters.

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• Next
The “Next” button validates your parameter and opens the following analysis step.
B- Edit band C – Marker values

• Back
The “Back” button validates your parameter and opens the following analysis step.
B- Edit band A - Lane definition

• Help menu
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Detect – Add marker values

The marker values process follows the band detection. This function allows the assignment of the molecular weight
marker’s values to the bands of the marker lane.

Note: you can either access marker value menu by clicking on the next button of the band detection or directly on
marker value tab.

The dashboard details the marker values parameters:

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• Load a marker
Click on the “Load a marker” button to open a marker value file.

A pop-up window displays the following menu:

Browse to specify the marker directory. Double click on the file name you want to load

• Create a marker
Click on the “Create a marker” button to create marker’s values.

Type your values, band to band, in a descending order. The OK button validates your data.

Note: A minimum of four values is necessary to validate the data.

Note: if an automatic calculation with immediate application of the standard values is carried out, it is not necessary
to enter all the bands given by the manufacturer's specifications, but only those which are commonly found on the
lanes of the gel.

You can save your molecular weights data and create your own marker library; To proceed, click on the “Save “
button:

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A pop-up window displays the following menu:

Browse to specify the directory. Type the file name and click on Save.
Select the lane corresponding to the molecular weight marker:

The migration curve is automatically displayed next to the lane’s profile:

The migration curve allows to check the detection of value application errors, distortion errors, bad separation
between the bands, or the quality of the standard itself.

Note: To delete the wrong data, you can either place the cursor arrow on the wrong value itself and click on it, or go
for the manual assignment.

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Note: The displayed migration curve is of the cubic spline type and must then include a minimum of 4 values.

Note: The minimum MW indicates the minimum molecular weight, which can be calculated, based on the marker’s
value assignment.

Note: The maximum MW indicates the maximum molecular weight, which can be calculated, based on the marker’s
value assignment.

Assign the marker values to the band

Assign manually the marker values to the lane by selecting the appropriate option:

For manual assignment, click first on the molecular weight’s value to be assigned. The value is highlighted in red.
Then, click on the corresponding lane. The value is assigned to the lane:

• Next
The “Next” button validates your parameter and opens the following analysis step.
C – Add marker values 2- Analyse - MW
A- results

• Back
The “Back” button validates your parameter and opens the following analysis step.

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 C – Marker values B- Band detection

• Edit the marker values

Click on the “Edit the marker values” button.

A pop-up window displays the following menu on which you can modify the marker values:

You can add, remove, and save your marker values;

• Help

Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Molecular weight results

The molecular weight results process follows the marker’s value assignment.

Note: you can either access the molecular weight results by clicking on the next button of the marker’s value
assignment or directly by clicking on the Molecular weight tab of the 2-Analyse-MW folder.

The dashboard details the molecular weight results parameters:

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• Back
The “Back” button validates your parameter and opens the following analysis step.
2- Analyse – MW 1- Detect
A-Molecular weight C- Marker values

• Help

Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Return to the image acquisition module

To return to the image acquisition module, select Home from the dash menu.

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Quantification analysis module

The software Quantification module features the quantification of spot in volume, percentage.

At the end of the process, you can have the following outputs:
- Lane’s volume and concentration

Detect – Lane definition

The Quantification module opens on the Lane definition dashboard of the Window definition process:

The dashboard details the lane definition parameters:

• Define a new lane

On the image, click on the top left corner of the lane, then drag to define the size of the analysis area. You can easily
adjust the size of the area by clicking on the tags surrounding the area and drag the selected border to the requested
size.

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The lane is defined by green lines, overlaid on the image. The area is surrounded by square anchors:

To resize the entire lane frame, drag an anchor point in or out. The opposite anchor point will remain fixed while the
frame expands or contracts. The frame will expand or contract from the centre.

To move the entire frame to a new position, position the mouse on the frame to obtain a cross cursor: Click and drag
the cursor to move the entire frame.

• Copy a lane
To copy a lane, select the lane in the lane list:

Click on the Copy button. The lane is then duplicated:

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The number of lanes is automatically modified in the lane list.
You can move the lane frame to a new position. In order to do so, position the mouse on the. Click and drag the
cursor to move the frame.

• Delete a lane
To delete a lane, select the lane in the lane list:

Click on the Delete button. The lane is then deleted.

The number of lanes is automatically modified in the lane list.

You can move the lane frame to a new position. In order to do so, position the mouse on the. Click and drag the
cursor to move the frame.

• Next
The “Next” button validates your parameter and opens the following analysis step.
1 A – Lane definition 1 B – Background subtraction

• Help menu
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

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Background subtraction
The background subtraction process follows the lane definition.
Image background interferes with quantification and data analysis such. Background could results from gel opacity,
random signal noise, opacity of the carrier medium (film, gel matrix, or blot matrix) or film fogging.

As image background interferes with quantification and data analysis, we recommend performing a background
subtraction before any peak volume quantification. The software has several approaches for subtracting background
intensity from gel images. You can either subtract background for the whole image or for individual lanes.

Note: As background subtraction permanently changes the image, this is not possible to save the image with a
processed background subtraction. However, the process can be saved by saving the complete analysis through the
Save analysis process.

The dashboard details the noise subtraction parameters:

• Rolling ball
The rolling ball method is named for a hypothetical ball that rolls along underneath the lane profile, removing the
intensity levels along the length of the lane.
The rolling ball subtraction method removes background levels along the length of the lanes according to the size of
a virtual ball. The ball is rolled under each profile of the image so its movement varies along the image. The ball
follows the profile trace, touching fewer points along the trace and removing the lower part of the points which
defined the subtracted background.

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The centre of gravity of the ball describes a curve:
This curve represents the noise to be subtracted.
The curve depends on the size of the ball and on the size of the peaks.

The size of the ball affect the position and movements of the centre of gravity and thus it determined how much
background will be subtracted. A smaller disk will more closely follow the profile trace, removing more background.
A disk radius that is too large will result in poor Subtract background. A disk radius that is too small may subtract
actual data.

The software calculates automatically the ideal parameter for background subtraction. This could be manually
modified by adjusting the spot size:

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To process the rolling ball background subtraction, click on the “Do rolling ball” button:
The changes will be automatically applied to the image.

Note: few seconds could be necessary to perform the background subtraction.

• Linear approach
The linear approach is a lane-based background subtraction. It allows to manually define the level of noise to be
subtracted on the lane profile. This option gives you the opportunity to set different background subtraction levels
for each lane.

Click on the “Linear ” button:

It opens the lane profile window:

In the profile parameters window, select the lane to perform the linear approach:

On the profile, click to define the background linear level you want to remove:

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Then, click on Subtract noise:
The changes will be automatically applied to the image and to the profile:

The linear approach is a lane-based background subtraction. You can set the same subtraction level for all lanes or
specify an individual subtraction level for the selected lane. Any changes you make will be automatically applied to
the image.

To apply the same subtraction level for all lanes, click on the “Apply to all lanes” button:

• Next
The “Next” button validates your parameter and opens the following analysis step.
1B – Background 1C-Spot separation
subtraction
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• Back
The “Back” button validates your parameter and opens the following analysis step.
1B – Background 1A - Lane definition
subtraction

• Help
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Spot separation
In order to measure the volume of a particular spot, you need:
• To define the boundary around the spot;
• To compare the intensity data inside the boundary with the data of other spots or of a standard.

A volume is the sum of the pixel intensity inside a defined boundary. The purpose of the spot separation is to define
this boundary.
The spot separation process follows the background subtraction.

Note: you can either access the spot separation function by clicking on the next button of the background
subtraction or directly by clicking on the spot separation of the Window definition folder.

The dashboard details the spot separation parameters:

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• Modify the spot separation
The software proposes by default an automatic predefined spot separation based on the band detection. You can
modify the default spot separation by selecting the “Modify the spot separation” option.

The default separation is illustrated on the lane’s profile:

The brackets illustrate the bands boundaries:

You can easily reposition a band’s boundaries. In order to do so, click on the bracket and drag the cursor:

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Drag the cursor until the area of the band that you want to define has been completely enclosed.

Note: When you release the mouse button, the band’s volume is automatically recalculated to take into account the
new area of interest.

To add a separation or delete a separation, select “Add Separation” or “Delete separation”from the profile’s
parameter menu:

Then, click on the band where you want to add a separation or on a current separation you want to delete:

• Next
The “Next” button validates your parameter and opens the following analysis step.
1C- Spot separation 2A- Quantity of reference

• Back
The “Back” button validates your parameter and opens the following analysis step.
1C- Spot separation 1 B – Background subtraction

• Help menu
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Spot quantification
You can use the Volume tools to quantitate bands, spots, arrays, and other image data

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The images taken by the system are displayed by your computer screen in the form of grey scale images. Our
systems convert the light signals from your gel or membrane samples into digital data.
A digital image is a finite set of pixels. The digital image contains a fixed number of rows and columns of pixels. Pixels
are the smallest individual element in an image, holding quantized values that represent the light intensity at any
specific point. The light intensity is graded into a grey scale. The word pixel is based on a contraction of pix
("pictures") and el (for "element").

Volume is use in the band or spot quantification process. The volume is the sum of all the pixels intensities included
in the defined area (window + separation). To measure the quantity of a particular band or spot, you need to define
a band or a spot area.

Quantification is based on the image in pixels whose intensity is coded on a scale.

- The scale has 65 536 grey levels for a 16-bit image
- The scale has 256 grey levels for an 8-bit image

The quantity (or density) of a spot is calculated from its volume. This is made of the sum of all pixel intensities
composing the spot

In other words, the spot quantity then depends on:

- The number of pixels inside the area of the spot
- The intensities of these points

V =  ni Ii

Image analysis allows comparison in between concentrated intense spots and weaker but more diffused bands.

Results are given in volumes that may be recalculated according to an OD of reference or a concentration master-
curve.

To measure the amount of a particular spot, you need to define the boundary around the spot and compare the
intensity data inside the boundary with the data of other spots or of a standard.

Quantity of reference
A volume is the total signal intensity inside a defined boundary drawn on a lane. The purpose of the volume of
reference is to use volumes of known concentration to calculate the unknown concentrations.

The volume of reference process follows the spot separation.

Note: you can either access the volume of reference function by clicking on the next button of the background
subtraction or directly by clicking on the volume of reference of the 2-Spot Quantification folder.

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The dashboard details the quantity of reference parameters:

• Quantity of reference
The calculation of the unknown concentrations is based:
On the calculated volumes
On the known concentration. The known concentration is the quantity of reference.

The quantity of reference could correspond to one or several spots.

The purpose of the quantity of reference is to define the known concentration:

In the “Quantity of reference” edit field, type the quantity of known concentration you want to have as a reference:

• Lane of reference
The lane of reference defines the lane of the known concentration.
Select the lane of reference from the list:

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If a single lane is selected, only the volumes of this reference lane will be used to calculate the relationship between
the volume and the quantity. The other concentrations are calculated based on the concentration/volume
relationship of this specific lane.

Illustration 1: 100% / lane 1 / all bands. Total concentration lane 1= 100%

If “All lanes” is selected, for each lane a new relationship between volume and quantity will be recalculated,
according to the band’s lane selected. For instance, the defined parameters are 100% for all band all lanes; the
results table could be as follows. Lane by lane, the total band concentration is 100%:

Illustration 2: 100% / all lanes / all bands. Total concentration all lanes= 100%

• Spot(s) of reference
The quantity of reference could correspond to one or several spots of the selected lane.
Select one or several spots of the lane of reference from the list:

Example

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Let’s consider the known concentration is 100% contains in all the spots of lane A. The settings should then be as
follows:

The results table indicates the following for lane 3A:

• Results table
In the result parameter window, you can select the lanes and the values to be displayed in the results tables:
- Concentration
- Volume

Note: You can select or unselect the lanes to display in the results table.

Note: To reposition the 1D profile window, position your cursor at the top of the box. The cursor appearance will
change to a multidirectional arrow symbol. You can then drag the box to a new position.

• Back
The “Back” button validates your parameter and opens the following analysis step.
1C – Spot separation 2A – Quantity of
reference

• Help menu
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Return to the image acquisition module

To return to the image acquisition module, select Home from the dash menu.

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191
Colony counting analysis module

Objectives and output

The Colony counting module features the calculation of the number of colonies and their characteristics (Automatic
colony counting) or the manual counting of colonies with the mouse (Manual colony counting).

At the end of the process, you can have the following outputs:
- Number of colonies of type I (detected as green)
- Number of colonies of type II (detected as red)

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Automatic counting

Detection
The detection process automatically identifies all the colonies for a defined area of analysis lanes. The colonies will
be automatically detected when you first access the automatic counting.
Two types of colonies can be detected:
• Type A called green type, overlaid on green on the image.
• Type B, called red type, overlaid on red on the image.

The dashboard details the Definition parameters:

 Define the area of analysis

 Adjust the detection parameters

DEFINE THE AREA OF ANALYSIS

You could define a circular, a rectangular or a free form area of analysis:

To define a free form, click on the first point of the area on the image. Move the cursor to define one edge of the area. Validate
this edge by clicking once. Then, repeats this procedure as many times as necessary.
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Select the “Square” option to obtain a circle instead of an ellipse or a square instead of a rectangle.

ADJUST THE DETECTION PARAMETERS

The detection parameters are summarised in the following bar graph:

The green and red areas represent the grey level range used to determine both kinds of colonies.
Click on the colored triangle and drag them to a new place to modify the detection range. A preview of the detection is displayed
on the image.

Note: you could also type the grey level range in the detailed green and red parameters:

RESULT TABLE
In the result parameter window, you can select the type of colony (both, only red, only green).

OPTION FOLDER
The option folder gathers the following functions:
 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Return to the Capt acquisition module

To return to the Capt acquisition module, select Home Exit from the dash menu.

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Manual counting
The manual Colony counting opens on this screen:

The dashboard details the manual counting parameters:

 Left click for green type colonies

 Right click for red type colonies
 Reset counting

LEFT CLICK FOR GREEN TYPE COLONIES

Click on the left mouse button to count a green type colony. The colony is then added to the green type counter:

Note: you can cancel a counted colony by clicking once again with the left mouse button.

RIGHT CLICK FOR RED TYPE COLONIES

Click on the right mouse button to count a red type colony. The colony is then added to the red type counter:

Note: you can cancel a counted colony by clicking once again with the right mouse button.

RESET COUNTING
The “Reset” button restores the counters to zero.

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OPTION FOLDER

The option folder gathers the following functions:

 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Return to the Capt acquisition module

To return to the Capt acquisition module, select Home Exit from the dash menu.

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Distance calculation module
Objectives and output

The Distance module features the calculation of electrophoretic distances according to markers or standards:
• in R.F. values (values between 0 and 1)
• in isoelectric points (pH units)

This function allows the assignment of RF values to the detected bands. To do so, an origin line and a front line
must be defined. Usually the origin is set to value 0 and the front to 1, but the software allows you to set your own
values for origin and front lines.

At the end of the process, you can have the following outputs:
- Profile and migration curve
- Calculated distance in between a front line and an end line.

1-Detect
A – Edit lane
The distance module opens on the Edit lane dashboard of the Detect process:

The dashboard details the lane edition parameters:

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 Lane direction
 Number of lanes
 Gap between the lanes

AREA OF INTEREST
On the image, click and drag to define the analysis area and to overlap the lanes. You can easily adjust the size of the area by
clicking on the tags surrounding the area and drag the selected border to the requested size.

The lanes are defined by green lines, overlaid on the gel image. The gel area is surrounded by square anchors:

To resize the entire lane frame, drag an anchor point in or out. The opposite anchor point will remain fixed while the frame
expands or contracts. The frame will expand or contract from the center.

To move the entire frame to a new position, position the mouse on the frame to obtain a cross cursor:

Click and drag the cursor to move the entire frame.

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Note: it is not necessary to include the well line in the area of interest window. The calculation of molecular weight does not
require this reference line.

LANE DIRECTION
Select the direction of the lanes from:
 Horizontal
 Or vertical

The lane direction is automatically modified on the image.

NUMBER OF LANES
Select the number of lanes:

The number of lane is automatically modified on the image.

GAP BETWEEN THE LANES

Define the gap between the lanes:

Short gap Large gap

The gap between the lanes is automatically modified on the image.

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RESET
The “Reset” button restores the default lane detection parameters.

NEXT

The “Next” button validates your parameter and opens the following analysis step.

A- Edit lane B – Edit band

OPTION FOLDER

The option folder gathers the following functions:

 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

B – Edit band

The Edit band process follows the lane definition:

The edit band process automatically identifies all the bands for the defined lanes. You can also manually mark the
bands on the image or on the lane’s profile. All bands will be automatically detected when you first access the band
detection process, based on default parameters.

The bands are marked by green lines, overlaid on the gel image.

Note: you can either access the lane definition menu by clicking on the next button of the lane definition or directly
on the band detection tab.

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The dashboard details the edit band parameters:

 Profile lane
 Arrow or square cursor detection

BAND DETECTION ON THE IMAGE

You can add or remove bands by clicking directly on the image. Place the cursor at the chosen location and click. The band is
immediately added or removed. The red line allows you to check band alignment between lanes.

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 BAND DETECTION ON THE PROFILE
The profile is calculated based on the average intensity of each row of pixels across the specified width of the lane. A lane profile
provides a visualisation of the intensity of the bands. Bands are represented by peaks.

You can add or remove bands by clicking directly on the profile. To proceed, select
the cursor type:

 The linear cursor has the shape of an arrow ( )

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 The rectangular cursor has the shape of a square ( )

Place the cursor at the chosen profile location and click. The detection line is automatically added or removed.

Note: For arrow cursor, the band is added at the cursor position.
For rectangular cursor, the band is added at the highest position within cursor bounds.

VISUALISATION OF THE PROFILES

In the profile parameter window, you can select the lanes’ profile to be displayed. To do so, just click on the profile name to
select or unselect the profiles.

You can also define the way the profiles are displayed:
- Only the profile
- Only the image
- Both the profile and the image
- In an horizontal way
- In a vertical way
- Stacked the profiles of the selected lanes

RESET
You can reset the detection by clicking on the “Reset” button. The detection lines will then be removed.

RE-DETECT
You can re-detect the bands by clicking the “Re-detect” button. The detection is based on the default parameters.

NEXT
The “Next” button validates your parameter and opens the following analysis step.

B- Edit band

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C – Distance

BACK
The “Back” button validates your parameter and opens the following analysis step.

B- Edit band

A - Lane definition

OPTION FOLDER
The option folder gathers the following functions:
 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

C – Distance
The Distance process follows the band detection.

This function allows the assignment of RF values to the detected bands. To do so, an origin line and a front line
must be defined. Usually the origin is set to value 0 and the front to 1, but the software allows you to set your own
values for origin and front lines.

Note: you can either access marker value menu by clicking on the next button of the band detection or directly on
marker value tab.

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The dashboard details the marker values parameters:

 Define the reference value

 Define the front value

DEFINE THE REFERENCE AND THE FRONT VALUES

An origin and a front line are displayed on the image:

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Click on the first line, keep pressed the left mouse button, and move it to the location for the origin line. Then, release the button.
Click on the second line and move it to the location for the migration front. Then, release the button. The R.F. values are assigned
to the bands.

Select the value for the origin and the value the end.

NEXT
The “Next” button validates your parameter and opens the following analysis step.

C – Distance

2- Analyse
A- results

BACK
The “Back” button validates your parameter and opens the following analysis step.

C - Distance

B- Band detection

OPTION FOLDER

The option folder gathers the following functions:

 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

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2- Analyse – Distance
A – Distance results

The Distance results process follows the marker’s value assignment.

Note: you can either access the molecular weight results by clicking on the next button of the marker’s value
assignment or directly by clicking on the Molecular weight tab of the 2-Analyse-MW folder.

The dashboard details the molecular weight results parameters:

BACK
The “Back” button validates your parameter and opens the following analysis step.

2- Analyse – MW
A-Molecular weight

1- Detect
C- Distance

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OPTION FOLDER

The option folder gathers the following functions:

 Help

HELP MENU
Click on the “Help” button. You automatically access the user manual at the chapter corresponding to the function

Return to the Capt acquisition module

To return to the Capt acquisition module, select Home Exit from the dash menu.

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