B.SC.

,
MICROBIOLOGY

SYLLABUS

FROM THE ACADEMIC YEAR

2023-2024

TAMILNADU STATE COUNCIL FOR HIGHER EDUCATION,

CHENNAI – 600 005
Contents
i. PO and PSO Description
ii. Methods of Evaluation & Methods of Assessment

iii. Semester Index.

iv. Subjects – Core, Elective, Nonmajor, Skill Enhanced, Ability Enhanced, Extension
Activity, Environment, Professional Competency

1) Course Lesson Box

2) Course Objectives
3) Units
4) Learning Outcome
5) Reference and Text Books
6) Web Sources
7) PO Mapping tables
LEARNING OUTCOMES-BASED CURRICULUM FRAMEWORK GUIDELINES BASED REGULATIONS FOR UNDER
GRADUATE PROGRAMME
Programme: B.Sc. MICROBIOLOGY
Programme
Code:
Duration: 3 Years (UG)
Programme PO1: Disciplinary knowledge: Capable of demonstrating comprehensive knowledge and
understanding of one or more disciplines that form a part of an undergraduate Programme
Outcomes: of study
PO2: Communication Skills: Ability to express thoughts and ideas effectively in writing
and orally; Communicate with others using appropriate media; confidently share one’s
views and express herself/himself; demonstrate the ability to listen carefully, read and
write analytically, and present complex information in a clear and concise manner to
different groups.
PO3: Critical thinking: Capability to apply analytic thought to a body of knowledge;
analyse and evaluate evidence, arguments, claims, beliefs on the basis of empirical
evidence; identify relevant assumptions or implications; formulate coherent arguments;
critically evaluate practices, policies and theories by following scientific approach to
knowledge development.
PO4: Problem solving: Capacity to extrapolate from what one has learned and apply
their competencies to solve different kinds of non-familiar problems, rather than replicate
curriculum content knowledge; and apply one’s learning to real life situations.
PO5: Analytical reasoning: Ability to evaluate the reliability and relevance of evidence;
identify logical flaws and holes in the arguments of others; analyze and synthesize data
from a variety of sources; draw valid conclusions and support them with evidence and
examples, and addressing opposing viewpoints.
PO6: Research-related skills: A sense of inquiry and capability for asking
relevant/appropriate questions, problem arising, synthesising and articulating; Ability to
recognise cause-and-effect relationships, define problems, formulate hypotheses, test
hypotheses, analyse, interpret and draw conclusions from data, establish hypotheses,
predict cause-and-effect relationships; ability to plan, execute and report the results of an
experiment or investigation
PO7: Cooperation/Team work: Ability to work effectively and respectfully with diverse
teams; facilitate cooperative or coordinated effort on the part of a group, and act together
as a group or a team in the interests of a common cause and work efficiently as a member
of a team
PO8: Scientific reasoning: Ability to analyse, interpret and draw conclusions from
quantitative/qualitative data; and critically evaluate ideas, evidence and experiences
from an open-minded and reasoned perspective.

PO9: Reflective thinking: Critical sensibility to lived experiences, with self awareness and
reflexivity of both self and society.

PO10 Information/digital literacy: Capability to use ICT in a variety of learning situations,

demonstrate ability to access, evaluate, and use a variety of relevant information
sources; and use appropriate software for analysis of data.

PO 11 Self-directed learning: Ability to work independently, identify appropriate

resources required for a project, and manage a project through to completion.

PO 12 Multicultural competence: Possess knowledge of the values and beliefs of

multiple cultures and a global perspective; and capability to effectively engage in a
multicultural society and interact respectfully with diverse groups.
 PO 13: Moral and ethical awareness/reasoning: Ability toembrace moral/ethical values
in conducting one’s life, formulate a position/argument about an ethical issue from
multiple perspectives, and use ethical practices in all work. Capable of demonstratingthe
ability to identify ethical issues related to one‟s work, avoid unethical behaviour such as
fabrication, falsification or misrepresentation of data or committing plagiarism, not
adhering to intellectual property rights; appreciating environmental and sustainability
issues; and adopting objective, unbiased and truthful actions in all aspects of work.

PO 14: Leadership readiness/qualities: Capability for mapping out the tasks of a team or
an organization, and setting direction, formulating an inspiring vision, building a team
who can help achieve the vision, motivating and inspiring team members to engage with
that vision, and using management skills to guide people to the right destination, in a
smooth and efficient way.

PO 15: Lifelong learning: Ability to acquire knowledge and skills, including „learning how
to learn‟, that are necessary for participating in learning activities throughout life,
through self-paced and self-directed learning aimed at personal development, meeting
economic, social and cultural objectives, and adapting to changing trades and demands
of work place through knowledge/skill development/reskilling.
Programme On successful completion of Bachelor of Physics with Computer Applications
Specific programme, the student should be able to:
Outcomes: PSO1: Disciplinary Knowledge: Understand the fundamental principles,
concepts, and theories related to physics and computer science. Also, exhibit
proficiency in performing experiments in the laboratory.
PSO2: Critical Thinking: Analyse complex problems, evaluate information,
synthesize information, apply theoretical concepts to practical situations, identify
assumptions and biases, make informed decisions and communicate effectively
PSO3: Problem Solving: Employ theoretical concepts and critical reasoning
ability with physical, mathematical and technical skills to solve problems, acquire
data, analyze their physical significance and explore new design possibilities.
PSO4: Analytical & Scientific Reasoning: Apply scientific methods, collect and
analyse data, test hypotheses, evaluate evidence, apply statistical techniques and
use computational models.
PSO5: Research related skills: Formulate research questions, conduct literature
reviews, design and execute research studies, communicate research findings and
collaborate in research projects.
PSO6: Self-directed & Lifelong Learning: Set learning goals, manage their own
learning, reflect on their learning, adapt to new contexts, seek out new knowledge,
collaborate with others and to continuously improve their skills and knowledge,
through ongoing learning and professional development, and contribute to the
growth and development of their field.

PO/PSO PSO1 PSO2 PSO3 PSO4 PSO5 PSO6

PO1 
PO2 
PO3 
PO4 
PO5 
PO6 
2. Highlights of the Revamped Curriculum:

 Student-centric, meeting the demands of industry & society, incorporating industrial

components, hands-on training, skill enhancement modules, industrial project, project with
viva-voce, exposure to entrepreneurial skills, training for competitive examinations,
sustaining the quality of the core components and incorporating application oriented content
wherever required.
 The Core subjects include latest developments in the education and scientific front,
advanced programming packages allied with the discipline topics, practical training,
devising statistical models and algorithms for providing solutions to industry / real life
situations. The curriculum also facilitates peer learning with advanced statistical topics in
the final semester, catering to the needs of stakeholders with research aptitude.
 The General Studies and Statistics based problem solving skills are included as mandatory
components in the ‘Training for Competitive Examinations’ course at the final semester, a
first of its kind.
 The curriculum is designed so as to strengthen the Industry-Academia interface and provide
more job opportunities for the students.
 The Statistical Quality Control course is included to expose the students to real life problems
and train the students on designing a mathematical model to provide solutions to the
industrial problems.
 The Internship during the second year vacation will help the students gain valuable work
experience, that connects classroom knowledge to real world experience and to narrow
down and focus on the career path.
 Project with viva-voce component in the fifth semester enables the student, application of
conceptual knowledge to practical situations. The state of art technologies in conducting a
Explain in a scientific and systematic way and arriving at a precise solution is ensured. Such
innovative provisions of the industrial training, project and internships will give students an
edge over the counterparts in the job market.
 State-of Art techniques from the streams of multi-disciplinary, cross disciplinary and inter
disciplinary nature are incorporated as Elective courses, covering conventional topics to the
latest DBMS and Computer software for Analytics.
ValueadditionsintheRevampedCurriculum:

Semester NewlyintroducedComponents Outcome/ Benefits

I FoundationCourse  Instill
To ease the transition of
learningfrom higher secondary confidenceamongstude
to nts
highereducation,providinganove  Createinterestforthesu
rviewofthepedagogyoflearningLi
bject
teratureandanalysingtheworldth
roughtheliterarylens
givesrisetoanewperspective.
I,II,III,IV SkillEnhancementpapers(Discipl  Industry
ine centric readygraduates
/Generic/Entrepreneurial)  Skilledhumanresource
 Studentsareequippedw
ithessentialskillsto
makethememployable
 Trainingonlanguagean
dcommunicationskillse
nablethestudents gain
knowledge and
exposureinthecompeti
tiveworld.

 Discipline centric
skillwillimprovetheTec
hnical knowhow
ofsolvingreallife
problems.
III,IV,V& VI Electivepapers  Strengthening
thedomainknowledge
 Introducing
thestakeholders to
theState-of
Arttechniquesfrom the
streamsofmulti-
disciplinary,crossdiscipl
inaryandinterdisciplina
rynature
 Emerging topics
inhigher

education/industry/co
mmunicationnetwork/
healthsectoretc.areintr
oducedwith
hands-on-training.
IV ElectivePapers  Exposuretoindustrymo
uldsstudentsintosoluti
onproviders
 GeneratesIndustryread
ygraduates
 Employmentopportuni
tiesenhanced
VSemester Electivepapers  Self-learning
isenhanced
 Applicationoftheconce
pttorealsituationisconc
eivedresulting
intangibleoutcome
VISemester Electivepapers
 Enriches the
studybeyondthe
course.
 Developingaresearchfr
amework
and
presenting their
independent and
intellectual

ideaseffectively.
ExtraCredits:  Tocatertotheneedsofp
ForAdvancedLearners/Honorsdegree eerlearners/research
aspirants
SkillsacquiredfromtheCourses Knowledge, Problem Solving, Analytical
ability,ProfessionalCompetency,ProfessionalCo
mmunicationandTransferrable Skill
 Credit Distribution for UG Programmes
Sem I Credi H Sem II Credi H Sem III Credit H Sem IV Credi H Sem V Credit H Sem VI Credit H
t t t
Part 1. 3 6 Part..1. 3 6 Part..1. 3 6 Part..1. 3 6 5.1 Core 4 5 6.1 Core 4 6
Language – Language – Language – Language – Course –\ Course –
Tamil Tamil Tamil Tamil CC IX CC XIII
Part.2 3 6 Part..2 3 6 Part..2 English 3 6 Part..2 3 6 5.2 Core 4 5 6.2 Core 4 6
English English English Course – Course –
CC X CC XIV
1.3 Core 5 5 2..3 Core 5 5 3.3 Core Course 5 5 4.3 Core 5 5 5. 3.Core 4 5 6.3 Core 4 6
Course – CC I Course – CC – CC V Course – CC Course Course –
III VII CC -XI CC XV
Core Industry
Module
1.4 Core 5 5 2.4 Core 5 5 3.4 Core Course 5 5 4.4 Core 5 5 5. 4.Core 4 5 6.4 Elective 3 5
Course – CC Course – CC – CC VI Course – Course –/ -VII Generic/
II IV CC VIII Project Discipline
with viva- Specific
voce
CC -XII
1.5 Elective I 3 4 2.5 Elective II 3 4 3.5 Elective III 3 4 4.5 Elective 3 3 5.5 3 4 6.5 Elective 3 5
Generic/ Generic/ Generic/ IV Generic/ Elective V VIII
Discipline Discipline Discipline Discipline Generic/ Generic/
Specific Specific Specific Specific Discipline Discipline
Specific Specific
1.6 Skill 2 2 2.6 Skill 2 2 3.6 Skill 1 1 4.6 Skill 2 2 5.6 3 4 6.6 1 -
Enhancement Enhancement Enhancement Enhancement Elective Extension
Course Course Course SEC-4, Course VI Activity
SEC-1 SEC-2 (Entrepreneurial SEC-6 Generic/
Skill) Discipline
Specific
1.7 Skill 2 2 2.7 Skill 2 2 3.7 Skill 2 2 4.7 Skill 2 2 5.7 Value 2 2 6.7 2 2
Enhancement Enhancement Enhancement Enhancement Education Professional
-(Foundation Course –SEC- Course SEC-5 Course SEC-7 Competency
Course) 3 Skill
3.8 E.V.S. - 1 4.8 E.V.S 2 1 5.8 2
Summer
Internship
/Industrial
Training
23 30 23 30 22 30 25 30 26 3 21 30
0
Total – 140 Credits
 11

Choice Based Credit System (CBCS), Learning Outcomes Based Curriculum

Framework (LOCF) Guideline Based Credit and Hours Distribution System
for all UG courses including Lab Hours
First Year – Semester-I

Part List of Courses Credit No. of

Hours
Part-1 Language – Tamil 3 6
Part-2 English 3 6
Part-3 Core Courses & Elective Courses [in Total] 13 14
Skill Enhancement Course SEC-1 2 2
Part-4 Foundation Course 2 2
23 30

Semester-II

Part List of Courses Credit No. of

Hours
Part-1 Language – Tamil 3 6
Part-2 English 3 6
Part-3 Core Courses & Elective Courses including laboratory [in Total] 13 14
Part-4 Skill Enhancement Course -SEC-2 2 2
Skill Enhancement Course -SEC-3 (Discipline / Subject Specific) 2 2
23 30
Second Year – Semester-III

Part List of Courses Credit No. of

Hours
Part-1 Language - Tamil 3 6
Part-2 English 3 6
Part-3 Core Courses & Elective Courses including laboratory [in Total] 13 14
Part-4 Skill Enhancement Course -SEC-4 (Entrepreneurial Based) 1 1
Skill Enhancement Course -SEC-5 (Discipline / Subject Specific) 2 2
E.V.S - 1
22 30

Semester-IV

Part List of Courses Credit No. of

Hours
Part-1 Language - Tamil 3 6
Part-2 English 3 6
Part-3 Core Courses & Elective Courses including laboratory [in Total] 13 13
Part-4 Skill Enhancement Course -SEC-6 (Discipline / Subject Specific) 2 2
 12

Skill Enhancement Course -SEC-7 (Discipline / Subject Specific) 2 2

E.V.S 2 1
25 30
Third Year
Semester-V
Part List of Courses Credit No. of
Hours
Part-3 Core Courses including Project / Elective Based 22 26
Part-4 Value Education 2 2
Internship / Industrial Visit / Field Visit 2 2
26 30

Semester-VI

Part List of Courses Credit No. of

Hours
Part-3 Core Courses including Project / Elective Based & LAB 18 28
Part-4 Extension Activity 1 -
Professional Competency Skill 2 2
21 30

Consolidated Semester wise and Component wise Credit distribution

Parts Sem I Sem II Sem III Sem IV Sem V Sem VI Total

Credits
Part I 3 3 3 3 - - 12
Part II 3 3 3 3 - - 12
Part III 13 13 13 13 22 18 92
Part IV 4 4 3 6 4 1 22
Part V - - - - - 2 2
Total 23 23 22 25 26 21 140

*Part I. II, and Part III components will be separately taken into account for CGPA calculation
and classification for the under graduate programme and the other components. IV, V have
to be completed during the duration of the programme as per the norms, to be eligible for
obtaining the UG degree.
 13

MethodsofEvaluation
ContinuousInternalAssessmentTest
InternalE Assignments 25 Marks
valuation Seminars
AttendanceandClassParticipation
ExternalE
EndSemesterExamination 75 Marks
valuation
Total 100 Marks
MethodsofAssessment
Recall(K1) Simpledefinitions,MCQ,Recallsteps,Conceptdefinitions
Understand/ MCQ,True/False,Shortessays,Conceptexplanations,Shortsummaryor
Comprehend(K2) overview
Suggestidea/conceptwithexamples,Suggestformulae, Solveproblems,
Application (K3)
Observe,Explain
Analyze(K4) Problem-solvingquestions,Finishaprocedureinmanysteps,Differentiate
betweenvariousideas,Mapknowledge
Evaluate(K5) Longer essay/Evaluationessay,Critiqueorjustifywithprosandcons
Checkknowledgeinspecificoroffbeatsituations,Discussion,Debatingor
Create(K6)
Presentations
 14

FIRST SEMESTER

Sl.N Course Course Credit Overal Total Marks

O Category distribution l contact
Credits Hours/week CI ESE Total
A
L T P S
1 Part –I Language - Tamil L 3 6 25 75 100
2 Part –II English L 3 6 25 75 100
3 Part -III CC-1 L 4 5 25 75 100
4 Part -III CC-2 P 4 5 40 60 100
5 Part -III AL-1 L 3 4 25 75 100
6 Part –IV SEC-1 (NME) L 2 2 25 75 100
7 Part –IV FC L 2 2 25 75 100
Total 23 30

SECOND SEMESTER

Sl.N Course Course Credit Overall Total contact Marks

O Category distribution Credits Hours/week
CIA ESE Total

L T P S
1 Part –I Language L 3 6 25 75 100
- Tamil
2 Part –II English L 3 6 25 75 100
3 Part -III CC-3 L 4 5 25 75 100
4 Part -III CC-4 P 4 5 40 60 100
5 Part -III AL-2 L 4 4 25 75 100
6 Part –IV SEC-2 L 2 2 25 75 100
(NME)
7 Part –IV SEC-3 L 2 2 25 75 100
24 30
Total

THIRD SEMESTER

Sl.NO Course Course Credit Overall Total contact Marks

Category distribution Credits Hours/week
L T P S CIA ESE Total
1 Part –I Languag L 3 6 25 75 100
e-
Tamil
 15

2 Part –II English L 3 6 25 75 100

3 Part –III CC-5 L 4 5 25 75 100
4 Part –III CC-6 P 4 5 40 60 100
5 Part –III AL-3 L 3 3 25 75 100
6 Part –IV SEC-4 L 2 2 25 75 100
7 Part –IV SEC-5 L 2 2 25 75 100
9 Part –IV E.V.S L - 1 25 75 100
Total 23 30

FOURTH SEMESTER
Sl.NO Course Course Course Credit Overall Total contact Marks
Category Code distribution Credits Hours/week
L T P S CI ESE Total
A
1 Part –I Language L 3 6 25 75 100
- Tamil
2 Part –II English L 3 6 25 75 100
3 Part –III 22MBUG CC VII L 4 4 25 75 100
CT4
4 Part –III 22MBUG CC VIII P 4 4 40 60 100
CP4
5 Part –III 22MBUG AL IV L 3 4 25 75 100
DE4
6 Part –IV 22MBUGS SEC-6 L 2 2 25 75 100
EC6
7 Part –IV 22MBUGS SEC-7 L 2 2 25 75 100
EC7
9 Part –IV EVS L 2 2 25 75 100
Total 25 30
 16

FIFTH SEMESTER

Sl. Course Course Credit distribution Overall Total contact Marks

NO Category Credits Hours/week
L T P S CIA ESE Total
1 Part -III CC- IX L 4 5 25 75 100
2 Part –III CC –X L 4 5 25 75 100

3 Part -III CC- XI P 4 5 40 60 100

4 Part -III Core course/ 4 5 25 75 100
Project with
viva- voce-
XII
5 Part -III Elective-5 L 3 4 25 75 100
6 Part -III Elective-6 L 3 4 25 75 100
7 Part -IV Value 2 2 25 75 100
Education
8 Part -IV Internship/ 2 - 25 75 100
Industrial
visit/ Field
visit

Total
26 30
 17

SIXTH SEMESTER

Course Course Course Credit distribution Overall Total Marks

Category Code Credits contact
Hours/week
L T P S CIA ESE Total

1 Part -III CC-XIII L 4 6 25 75 100

2 Part -III CC-XIV L 4 6 25 75 100
3 Part -III CC-XV P 4 6 40 60 100
4 Part -III Elective-7 L 3 5 25 75 100
5 Part -III Elective-8 L 3 5 25 75 100
6 Part -IV Extension 1 - - - -
activity

7 Part -IV Professional L 2 2 25 75 100

competency
skill
Total 21 30
Credit Distribution for UG MICROBIOLOGY
S.No Part Course Details Credit
1 III Core(15x4) 60
2 Elective Generic/ Discipline Specific Elective(8x3=24) 24
3 I& II Language & English 24
(Lang - 4x3=12
Eng - 4x3=12)
4 NME(2x2) 4
5 EVS(1x2) 2
6 Value Education(1x2) 2
7 Extension Activity(1x1) 1
8  Ability Enhancement [AECC]- Soft Skill(4x2=8) 8
IV  Skill Enhancement Course [4 Courses x 2 credits 9
=8 credits ] SEC-4 – 1 Credit
 Summer internship/ Industrial training (2x1=2 2
credits)
 Foundation course 2
 Professional Competency Skill 2
141
Remarks: English Soft Skill Two Hours Will be handled by English Teachers
(4+2 = 6 hours for English).
 18

Subject Subject Name Category L T P S Cr Inst. Marks

Code edi Hours CIA Exter Total
ts nal
22MBUGC FUNDAMENTALS Core Y - - - 4 5 25 75 100
T1 OF Course –
MICROBIOLOGY 1
AND
MICROBIAL
DIVERSITY
Course Objectives
CO1 Learn the fundamental principles about different aspects of Microbiology including recent
developments in the area.

CO2 Describe the structural organization, morphology and reproduction of microbes.

CO3 Explain the methods of cultivation of microbes and measurement of growth.

CO4 Understand the microscopy and other basic laboratory techniques – culturing, disinfection
and sterilization in Microbiology.

CO5 Compare and contrast the different methods of sterilization.

UNIT Details No.of Course

Hour Objectives
s
I History and Evolution of Microbiology, Classification – Three 12 CO1
kingdom, five kingdom, six kingdom and eight kingdom.
Microbial biodiversity: Introduction to microbial biodiversity-
ecological niche. Basic concepts of Eubacteria, Archaebacteria
and Eucarya. Conservation of Biodiversity.
II General characteristics of cellular microorganisms (Bacteria, 12 CO2
Algae, Fungi and Protozoa) and acellular microorganisms -
(Viruses, Viroids, Prions), Differences between prokaryotic and
eukaryotic microorganisms. Structure of Bacterial cell wall, cell
membrane, capsule, flagella, pili, mesosomes, chlorosomes,
phycobilisomes, spores, and gas vesicles. Structure of fungi
(Mold and Yeast), Structure of microalgae.
III Bacterial culture media and pure culture techniques. Mode of 12 CO3
cell division, Quantitative measurement of growth. Anaerobic
culture techniques.
 19

IV Microscopy – Simple, bright field, dark field, phase contrast, 12 CO4

fluorescent, electron microscope – TEM & SEM, Confocal
microscopy, and Atomic Force Microscopy. Stains and staining
methods.
V Sterilization–moist heat - autoclaving, dry heat – Hot air oven, 12 CO5
radiation – UV, Ionization, filtration – membrane filter and
disinfection, antiseptic; Antimicrobial agents.
Total 60
Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Study the historical events that led to the discoveries and PO5, PO6, PO10
inventions and understand the Classification of
Microorganisms.
CO2 Gain Knowledge of detailed structure and functions of PO10
prokaryotic cell organelles.
CO3 Understand the various microbiological techniques, different PO11
types of media, and techniques involved in culturing
microorganisms.
CO4 Explain the principles and working mechanism of different PO4, PO11
microscopes/Microscope, their function and scope of
application.
CO5 Understand the concept of asepsis and modes of sterilization PO4, PO11
and disinfectants.

Text Books
Pelczar.M. J., Chan E.C.S. and Noel. R.K. (2007). Microbiology. 7 thEdition.,McGraw –
1
Hill, New York.
Willey J., Sherwood L., and Woolverton C. J., (2017). Prescott’s Microbiology. 10th
2
Edition., McGraw-Hill International edition.
Tortora, G.J., Funke, B.R., Case,C.L. (2013). Microbiology. An Introduction 11 thEdition.,
3
A La Carte Pearson.
Salle. A.J (1992). Fundamental Principles of Bacteriology. 7thEdition., McGraw Hill
4
Inc.New York.
Boyd, R.F. (1998). General Microbiology,2ndEdition., Times Mirror, Mosby
5
CollegePublishing, St Louis.
References Books
1 Jeffrey C. Pommerville., Alcamo’s Fundamentals of Microbiology (9 thEdition). Jones
&Bartlett learning 2010.
2 Stanier R.Y, Ingraham J. L., Wheelis M. L., and Painter R. R. (2010). General
 20

Microbiology, 5thEdition., MacMillan Press Ltd

3 Tortora, G.J., Funke, B.R. and, Case, C.L (2013). Microbiology-An Introduction,
11thEdition., Benjamin Cummings.
4 Nester E., Anderson D., Roberts C. E., and Nester M. (2006). Microbiology-A Human
Perspective, 5thEdition., McGraw Hill Publications.
5 Madigan M.T., Martinko J.M., Stahl D.A, and Clark D. P. (2010). Brock - Biology of
Microorganisms, 13th Edition Benjamin-Cummings Pub Co.
Web Resources
https://www.cliffsnotes.com/study-guides/biology/microbiology/introduction-to-
1
microbiology/a-brief-history-of-microbiology
2 https://www.keyence.com/ss/products/microscope/bz-x/study/principle/structure.jsp
3 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604941/#
4 https://bio.libretexts.org/@go/page/9188
https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-
5
nutrition/
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
 21

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M M M
CO2 M M
CO3 S
CO4 M S
CO5 M S

Subject Subject Name Category L T P S Cr Inst. Marks

Code edi Hou CIA External Total
ts rs
22MBU PRACTICAL I - Core - - Y - 4 5 40 60 100
GCP1 FUNDAMENTAL Course
S OF II-
MICROBIOLOG Practical
Y AND I
MICROBIAL
DIVERSITY

Course Objectives
CO1 Acquire knowledge on Cleaning of glass wares, GLP and sterilization.

CO2 Gain knowledge on media preparation and cultural characteristics.

CO3 Learn the pure culture technique

CO4 Learn the microscopic techniques and staining methods.

CO5 Acquire knowledge on stain and staining methods

 22

UNIT Details No.of Course

Hours Objectives
I Cleaning of glass wares, Microbiological good laboratory 12 CO1
practice and safety. Sterilization and assessment of sterility–
Autoclave, hot air oven, and membrane filtration.
II Media preparation: liquid media, solid media, semi-solid 12 CO2
media, agar slants, agar deeps, agar plates.
III Preparation of basal, differential, enriched, enrichment, 12 CO3
transport, and selective media preparation- quality control
of media, growth supporting properties, sterility check of
media.
Pure culture techniques: streak plate, pour plate, decimal
dilution.
IV Culture characteristics of microorganisms: growth on 12 CO4
different media, growth characteristics, and description.
Demonstration of pigment production.
Microscopy: light microscopy and bright field microscopy.
V Staining techniques: smear preparation, simple staining, 12 CO5
Gram’s staining and endospore staining.
Study on Microbial Diversity using Hay Infusion Broth-Wet
mount to show different types of microbes, hanging drop.
Total 60
Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Practice sterilization methods; learn to prepare media and their
PO4, PO7, PO8,
quality control. PO9, PO11
CO2 Learn streak plate, pour plate and serial dilution and pigment
PO4, PO7, PO8,
production of microbes. PO9
CO3 Understand Microscopy methods, different Staining PO4, PO7, PO8,
techniques and motility test. PO9, PO11
CO4 Observeculture characteristics of microorganisms. PO4, PO7, PO8,
PO9
CO5 Study on Microbial Diversity using Hay Infusion Broth-Wet PO4, PO7, PO8,
mount PO9
Text Books

James G Cappucino and N. Sherman MB(1996). A lab manual Benjamin Cummins,

1
New York 1996.
2 Kannan. N (1996). Laboratory manual in General Microbiology. Palani Publications.
 23

3 Sundararaj T (2005). Microbiology Lab Manual (1st edition) publications.

Gunasekaran, P. (1996). Laboratory manual in Microbiology. New Age International
4
Ld., Publishers, New Delhi.
R C Dubey and D K Maheswari (2002). Practical Microbiology. S. Chand
5
Publishing.
References Books
1 Atlas.R (1997). Principles of Microbiology, 2nd Edition, Wm.C.Brown publishers.
Amita J, Jyotsna A and Vimala V (2018). Microbiology Practical Manual. (1 st
2
Edition). Elsevier India
3 Talib VH (2019). Handbook Medical Laboratory Technology. (2nd Edition). CBS
Wheelis M, (2010). Principles of Modern Microbiology, 1st Edition. Jones and
4
Bartlett Publication.
5 Lim D. (1998). Microbiology, 2ndEdition, WCB McGraw Hill Publications.
Web Resources
http://www.biologydiscussion.com/micro-biology/sterilisation-and-disinfection-
1
methods-and-principles-microbiology/24403.
2 https://www.ebooks.cambridge.org/ebook.jsf?bid=CBO9781139170635
3 https://www.grsmu.by/files/file/university/cafedry//files/essential_microbiology.pdf
4 https://microbiologyinfo.com/top-and-best-microbiology-books/
https://www.cliffsnotes.com/studyguides/biology/microbiology/introduction-to-
5
microbiology/a-brief-history-of-microbiology
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
 24

Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M L M L M
CO2 S L L L
CO3 S M M L M
CO4 S M L L
CO5 S M L L

Subject Subject Category L T P S Cre Inst. Marks

Code Name dits Hour CI Exter Total
s A nal
22MBUGDE1 BASIC AND Elective Y - - - 3 4 25 75 100
CLINICAL Generic /
BIOCHEMI Discipline
STRY Specific
Elective-I

Course Objectives
CO1 Attain thorough knowledge on carbohydrates and lipids, their characteristic properties
and organization in carrying out all the living functions which constitute the life.

CO2 Explain the biological activity of amino acids and proteins.

CO3 Identify the metabolic errors in enzymes of carbohydrates and lipids.

CO4 Describe the disorders in amino acid metabolism.

CO5 Interpret the consequences, biochemical, clinical features, diagnosis and treatment of
metabolic diseases of day today life.

UNIT Details No.of Course

Hours Objectives
I Biomolecules -Carbohydrate – General properties, function, 12 CO1
 25

structure, classification– monosaccharides (Glucose, Fructose,

Galactose), Oligoaccharides (Sucrose, Maltose, Lactose) and
polysaccharides (Starch, Glycogen,) and biological
significance. Lipids – General properties, functions, structure,
classification (Simple, Derived and Complex), Cholesterol,
LDL, HDL – biological significance.
II Biomolecules - Amino acids – General properties, functions, 12 CO2
structure, classification and biological significance. Proteins–
General structure, Properties, functions, classification and
biological significance.
III Disorders of Metabolism: Disorders of carbohydrate 12 CO3
metabolism: diabetes mellitus,ketoacidosis, hypoglycemia,
glycogen storage diseases, galactosemia and lactose
intolerance. Disorders of lipid metabolism:
hyperlipidemia, hyperlipoproteinemia, hypercholesterolemia,
hypertriglyceridemia,sphingolipidosis.
IV Disorders of Metabolism: Disorders of amino acid 12 CO4
metabolism:alkaptonuria, phenylketonuria, phenylalaninemia,
homocystineuria, tyrosinemia, aminoacidurias.
V Evaluation of organ function tests: Assessment and clinical 12 CO5
manifestations of renal, hepatic, pancreatic, gastric and
intestinal functions.
Diagnostic enzymes: Principles of diagnostic enzymology.
Clinical significance of aspartate aminotransferase, alanine
aminotransferase, creatine kinase, aldolase and lactate
dehydrogenase.

Total 60
 26

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Explain the structure, classification , biochemical functions PO1
and significance of carbohydrates and lipids
CO2 Differentiate essential and non-essential amino acids, PO1
biologically important modified amino acids and their
functions, Illustrate the role, classification of Proteins and
recognize the structural level organization of proteins, its
functions and denaturation.
CO3 Assess defective enzymes and Inborn errors. Recognize PO4, PO5, PO6
diseases related to carbohydrate and lipid metabolism.
CO4 Discuss and evaluate the pathology of aminoacid metabolic PO4, PO5, PO6
disorders.
CO5 Appraise the imbalances of enzymes in organ function and PO5, PO6, PO9
relate the role of Clinical Biochemistry in screening and
diagnosis.

Text Books
Satyanarayana, U. and Chakrapani, U(2014).Biochemistry,4th Edition, Made Simple
1 Publisher.

Jain J L, Sunjay Jain and Nitin Jain (2016).Fundamentals of Biochemistry, 7th Edition,
2 S Chand Company.

AmbikaShanmugam’s (2016). Fundamentals of Biochemistry for Medical Students, 8 th

3 Edition. Wolters Kluwer India Pvt Ltd.

Vasudevan. D.M.Sreekumari.S, Kannan Vaidyanathan (2019). Textbook Of

4 Biochemistry For Medical Students. Kindle edition, Jaypee Brothers Medical
Publishers
Jeremy M. Berg,LubertStryer, John L. Tymoczko, Gregory J. Gatto (2015).
5
Biochemistry, 8th edition. WH Freeman publisher.
References Books
AmitKessel&Nir Ben-Tal (2018). Introduction to Proteins: structure, function and
1
motion. 2ndEdition, Chapman and Hall.
David L. Nelson and Michael M. Cox (2017).Lehninger Principles of Biochemistry,
2
7thEdition W.H. Freeman and Co., NY.
LupertStyrer, Jeremy M. Berg, John L. Tymaczko, Gatto Jr., Gregory J (2019).
3
Biochemistry. 9thEdition ,W.H.Freeman& Co. New York.
4. Donald Voet, Judith Voet, Charlotte Pratt (2016). Fundamentals of Biochemistry: Life
 27

at the Molecular Level, 5th Edition, Wiley.

Joy PP, Surya S. and AswathyC (2015). Laboratory Manual of Biochemistry, Edition
5.
1.,Publisher:Kerala agricultural university.
Web Resources
1 https://www.abebooks.com › plp
2 https://kau.in/document/laboratory-manual-biochemistry
3 https://metacyc.org
4 https://www.medicalnewstoday.com
5 https://journals.indexcopernicus.com
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehen MCQ, True/False, Short essays, Concept explanations, Short summary or overview
d (K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyze Problem-solving questions, Finish a procedure in many steps, Differentiate between
(K4) various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M
CO2 M
CO3 S S S
CO4 S S S
CO5 S S S
 28

Subject Subject Category L T P S Cre Inst. Marks

Code Name dits Hour CI Exter Total
s A nal
22MBUGSEC1 Social and Skill Y - - - 2 2 25 75 100
Preventive enhance
medicine ment
Course
SEC - 1
(NME)
Course Objectives

CO1 Describe the concepts of health and disease and their social determinants

CO2 Summarize the health management system

CO3 Know about the various health care services

CO4 Outline the goals of preventive medicine

CO5 Gain knowledge about alternate medicine

UNIT Details No.of Course

Hours Objectives
I Introduction to social medicine: 6 CO1
History of social medicine-concepts of health and disease-
social determinants of health and disease-Health and quality
of life-Health information system- measures of population
health-health policies.
II Health management: 6 CO2
Applications of behavioral sciences and psychology in health
management- nutritional programs for health management-
water and sanitation in human health-national programs for
communicable and non-communicable diseases-
environmental and occupational hazards and their control.
III Health care and services: 6 CO3
Health care of the community-information, education,
communication and training in health-maternal & child
health-school health services- Geriatrics-care and welfare of
the aged-mental health-health services through general
practitioners.
 29

IV Preventive medicine: 6 CO4

Introduction- role of preventive medicine- levels of
prevention-Risk assessment in communities and vulnerable
population –surveillance, monitoring and reporting of disease
outbreaks - forecasting and control measures in community
setting – early detection methods.
V Prevention through alternate medicine: 6 CO5
Unani, Ayurveda, Homeopathy, Naturopathy systems in
epidemic and pandemic outbreaks. International health
regulations. Infectious disease outbreak case studies and
precautionary response during SARS and MERS coronavirus,
Ebola and novel SARS-COV2 outbreaks.
Total 30

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Identify the health information system PO1,PO5, PO6
CO2 Associate various factors with health management system PO1,PO2, PO3,PO5,
PO6, PO9
CO3 Choose the appropriate health care services PO1,PO5, PO6
CO4 Appraise the role of preventive medicine in community PO4,PO5, PO6
setting
CO5 Recommend the usage of alternate medicine during PO1,PO5, PO6
outbreaks
Text Books
1. Park.K (2021). Textbook of preventive and social medicine, 26th edition.
BanarsidasBhanot publishers.

2. Mahajan& Gupta (2013). Text book of preventive and social medicine, 4thedition.
Jaypeebrothers medical publishers.

3. Chun-Su Yuan, Eric J. Bieber, Brent Bauer (2006). Textbook of Complementary and
Alternative Medicine. Second Edition. Routledge publishers.
4. Vivek Jain (2020). Review of Preventive and Social Medicine: Including Biostatics.
12th edition, Jaypee Brothers Medical Publishers.
5. Lal Adarsh Pankaj Sunder (2011). Textbook of Community Medicine: Preventive and
Social Medicine, CBS publisher.
References Books
1 Howard Waitzkin, Alina Pérez, Matt Anderson (2021). Social Medicine and the
coming Transformation. First Edition. Routledge publishers.
 30

2 GN Prabhakara (2010). Short Textbook of Preventive and Social Medicine. Second

Edition. Jaypee publishers.

3 Jerry M. Suls, Karina W. Davidson, Robert M. Kaplan (2010).Handbook of Health

Psychology and BehavioralMedicine.Guilford Press.

4 Marie Eloïse Muller, Marie Muller, MarthieBezuidenhout, KarienJooste (2006).Health

Care Service Management. Juta and Company Ltd.

5 Geoffrey Rose (2008).Rose's Strategy of Preventive Medicine: The Complete.OUP

Oxford.
Web Resources
1 https://www.omicsonline.org/scholarly/social--preventive-medicine-journals-articles-
ppts-list.php
2 https://www.teacheron.com/online-md_preventive_and_social_medicine-tutors
3 https://www.futurelearn.com
4 https://www.healthcare-management-degree.net
5 https://www.conestogac.on.health-care-administration-and-service-management
Methods of Evaluation

Continuous Internal Assessment Test

Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
 31

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S
CO2 S S M S S M
CO3 M S S
CO4 S S S M
CO5 S S S

SEMESTER II

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CI Exter Total
s A nal
22MBU MICROBIAL Core Y - - - 4 5 25 75 100
GCT2 PHYSIOLOGY Course III
AND
METABOLISM

Course Objectives
CO1 Study the basic principles of microbial growth.

CO2 Understand the basic concepts of aerobic and anaerobic metabolic pathways.

CO3 Analyze the role of individual components in overall cell function.

CO4 Provide information on sources of energy and its utilization by microorganisms.

CO5 Study the different types of metabolic strategies.

Unit Details No.of Course

Hours Objectives
I Physiology of microbial growth: Batch – continuous - synchronous 12 CO1
cultures; Growth Curve and measurement method (turbidity,
biomass, and cell count). Control of microbial growth.
 32

II Nutrition requirements - Photoautotrophs, Photoorganotrophs, 12 CO2

Chemolithotrophs (Ammonia, Nitrite, Sulfur, Hydrogen, Iron
oxidizing Bacteria), Chemoorganotrophs. Nutrition transport
mechanisms – Passive diffusion and Active transport. Factors
affecting microbial growth.
III An overview of Metabolism - Embden Meyerhof Pathway, Entner- 12 CO3
Doudoroff Pathway, Pentose Phosphate Pathway, Tricarboxylic
Acid Cycle. Electron Transport Chain and Oxidative
Phosphorylation. ATP synthesis. Fermentation-Homolactic
Fermentation, Heterolactic Fermentation, Mixed Acid Fermentation,
Butanediol Fermentation.
IV Photosynthesis - An Overview of chloroplast structure. 12 CO4
Photosynthetic Pigments, Light Reaction-Cyclic and non-cyclic
Photophosphorylation. Dark Reaction - Calvin Cycle.
V Bacterial reproduction - Binary fission, Budding, Reproduction 12 CO5
through conidia, cyst formation, endospore formation. Fungi asexual
and sexual reproduction, Microalgae reproduction. Asexual and
sexual reproduction of protozoa.
Total 60

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Describe microorganisms based on nutrition. PO6, PO9

CO2 Know the concept of microbial growth and identify the factors PO6, PO7, PO9
affecting bacterial growth.

CO3 Explain the methods of nutrient uptake. PO6, PO9

CO4 Describe anaerobic and aerobic energy production. PO6, PO9

CO5 Elaborate on the process of bacterial photosynthesis and PO6, PO9

reproduction.

Text Books
Schlegal, H.G. (1993). General Microbiology.,7 th Edition, Press syndicate of the
1 University of Cambridge.

2 RajapandianK.(2010). Microbial Physiology, Chennai: PBS Book Enterprises India.

 33

MeenaKumari. S. Microbial Physiology, Chennai 1st Edition MJP Publishers 2006.

3

Dubey R.C. and Maheswari, S. (2003). A textbook of Microbiology, New Delhi: S.

4 Chand & Co.

S. Ram Reddy, S.M. Reddy (2008). Microbial Physiology. Anmol Publications Pvt Ltd.
5

References Books

Robert K. Poole (2004). Advances in Microbial Physiology, Elsevier Academic Press,

1 New York, Volume 49.

Kim B.H., Gadd G.M. (2008). Bacterial Physiology and Metabolism. Cambridge
2 University Press, Cambridge.

Daniel R. Caldwell. (1995). Microbial Physiology & Metabolism Wm.C. Brown

3
Communications, Inc. USA.
Moat, A.G and J.W Foaster (1995). Microbial Physiology, 3 rd edition. Wiley – LISS, A
4 John Wiley & Sons. Inc. Publications.

BhanuShrivastava. (2011). Microbial Physiology and Metabolism: Study of Microbial

5
Physiology and Metabolism. Lambert academic Publication.

Web Resources

https://sites.google.com/site/microbial physiologyoddsem/teaching-contents
1

https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-Nutrition
2

https://onlinecourses.swayam2.ac.in/cec20_bt14/preview
3

http://web.iitd.ac.in/~amittal/2007_Addy_Enzymes_Chapter.pdf
4

5 https://www..frontiersin.org.microbial-physiology-and-metabolism
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
 34

External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M M
CO2 M L M
CO3 M M
CO4 M M
CO5 M M

Subject Subject Name Catego L T P S Cre Inst. Marks

Code ry dits Hours CIA Exter Total
nal
22MBU CCIV- - - Y - 4 5 40 60 100
GCP2 MICROBIAL CORE
PHYSIOLOGY PRAC
AND TICAL
METABOLISM II
 35

Course Objectives
CO1 Understand the principles of motility test.

CO2 Understand the basic concepts of staining methods.

CO3 Learn the bacterial count using different methods and anaerobic culture.

CO4 Study the morphological demonstration of microorganisms and identification.

CO5 Study the biochemical identification of the bacteria.

UNIT Details No.of Course

Hours Objectives
I Motility demonstration: hanging drop, wet mount preparation, 12
semi-solid agar, Craigie’s tube method. Staining techniques: CO1
Smear preparation, permanent specimen preparation, Capsular,
and Acid-fast staining
II Direct counts – Direct cell count (Petroff-Hausser counting 12 CO2
chamber), Turbidometry. Viable count - pour plate, spread plate.
Bacterial growth curve.
III Anaerobic culture methods. Antibiotic sensitivity testing: Disc 12 CO3
diffusion test- quality control with standard strains.
IV Morphological variations in algae, fungi and protozoa. 12 CO4
Micrometry: Demonstration of the size of yeast, fungal filaments
and protozoa.
V Methods of bacterial identification- morphological, 12 CO5
physiological, and biochemical methods - IMViC test, H2S, TSI,
Oxidase, catalase, urease test, and Carbohydrate fermentation
test.Maintenance of pure culture, paraffin method, stab culture,
maintenance of mold culture.
Total 60
Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Describe hanging drop, wet mount preparation, semi-solid agar, PO6, PO7, PO8, PO9,
Craigie’s tube method. PO11

CO2 Demonstrate Smear preparation, permanent specimen PO6, PO7, PO8, PO9,
preparation, Capsular, and Acid-fast staining. PO11
 36

CO3 Explain antibiotic sensitivity testing: Disc diffusion test- quality PO6, PO7, PO8, PO9,
control with standard strains. PO11

CO4 Describe demonstration of the size of yeast, fungal filaments and PO6, PO7, PO8, PO9,
protozoa. PO11

CO5 Elaborate on the bacterial identification- morphological, PO6, PO7, PO8, PO9,
physiological, and biochemical methods. PO11

Text Books
James G Cappucino and N. Sherman MB (1996). A lab manual Benjamin Cummins, New
1
York .
Kannan. N (1996).Laboratory manual in General Microbiology. Palani Publications.
2

Sundararaj T (2005). Microbiology Lab Manual (1st edition) publications.

3

Gunasekaran. P (2007). Laboratory manual in Microbiology. New age international

4
publisher.
Elsa Cooper (2018). Microbial Physiology: A Practical Approach. Callisto Reference
5
publisher.
References Books
DavidWhite., James Drummond., Clay Fuqua (2012) Physiology and Biochemistry of
1 Prokaryotes. 4th Ed. Oxford University Press, New York.

Robert K. Poole (2004). Advances in Microbial Physiology, Elsevier Academic Press,

2 New York, Volume 49.

Kim B.H., Gadd G.M. (2008). Bacterial Physiology and Metabolism. Cambridge
3 University Press, Cambridge.

Dawes, I.W and Sutherland L.W (1992). Microbial Physiology (2 nd edition), Oxford
4 Blackwell Scientific Publications.

Moat, A.G and J.W Foaster, (1995). Microbial Physiology, 3 rd edition. Wiley – LISS, A
5 John Wiley & Sons. Inc. Publications.

Web Resources
https://sites.google.com/site/microbial physiologyoddsem/teaching-contents
1

2 https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-Nutrition
 37

3 https://onlinecourses.swayam2.ac.in/cec20_bt14/preview
4 https://www.studocu.com/microbial-physiology-practicals
5 https://www.agr.hokudai.ac.jp/microbial-physiology
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
40 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 60 Marks
Evaluation
Total 100 Marks
Methods of Assessment

Simple definitions, MCQ, Recall steps, Concept definitions

Recall (K1)

Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6) Presentations.

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M L M L M
CO2 M M L M L
CO3 L M M L M
CO4 L M M M M
CO5 M M M M M
 38

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CI Exter Total
s A nal
 39

22MBUGDE BIO Elective Y - - - 3 4 25 75 100

2 INSTRUMENTA Generic /
TION Discipline
Specific
Elective II
Course Objectives

CO1 Understand the analytical instruments and study the basic principles in the field of
sciences.

CO2 To gain knowledge about principles of spectroscopy

CO3 Understand the analytical techniques of Chromatography and electrophoresis

CO4 To understand the principle of different types of scans used in medical diagnosis

CO5 To gain information about the principles of radioactivity and its measurements

Unit Details No.of Course

Hours Objectives
I Basicinstruments:pH meter, Buffer of biological importance, 12 CO1
Centrifuge- Preparative, Analytical and Ultra, Laminar Air
Flow, Autoclave, Hot Air Oven and Incubator. Biochemical
calculations-preparations of Molar solutions - Buffers-
Phosphate, Acetate, TE, TAE- calculation of Normality ,PPM-
Ammonium sulphate precipitation.
II Spectroscopic Techniques:Spectroscopic Techniques: 12 CO2
Colorimeter, Ultraviolet and visible, Infra red and Mass
Spectroscopy.
III Chromatographic and Electrophoresis 12 CO3
Techniques:Chromatographic Techniques: Paper, Thin Layer,
Column, HPLC and GC. Electrophoresis Techniques: Starch
Gel, AGE, PAGE.
IV Imaging techniques:Principle, Instrumentation and application of 12 CO4
ECG, EEG, EMG, MRI, CT and PET scan radioisotopes.
V Fluorescence and radiation based techniques:Spectrofluorimeter, 12 CO5
Flame photometer, Scintillation counter, Geiger Muller counter,
Autoradiography.
Total 60
 40

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Gain knowledge about the basics of instrumentation. PO1,PO4,PO11
CO2 Exemplify the structure of atoms and molecules by using the PO4,PO10,PO11
principles of spectroscopy.
CO3 Evaluate by separating and purifying the components. PO4,PO7,PO11
CO4 Understand the need and applications of imaging techniques. PO7,PO8,PO11
CO5 Categorize the working principle and applications of PO10,PO11
fluorescence and radiation.

Text Books
1. Jayaraman J (2011). Laboratory Manual in Biochemistry, 2 nd Edition. Wiley Eastern
Ltd., New Delhi .
2. Ponmurugan. P and Gangathara PB (2012). Biotechniques.1stEdition. MJP publishers.
3 Veerakumari, L (2009).Bioinstrumentation- 5 thEdition -.MJP publishers.
4 Upadhyay, Upadhyay and Nath (2002). Biophysical chemistry – Principles and
techniques 3rd Edition. Himalaya publishing home.
5 Chatwal G and Anand (1989). Instrumental Methods of Chemical Analysis. S.Himalaya
Publishing House, Mumbai.
References Books
1 Rodney.F.Boyer (2000). Modern Experimental Biochemistry, 3 rd Edition. Pearson
Publication.
2 SkoogA.,WestM (2014). Principles of Instrumental Analysis – 14 th Edition
W.B.SaundersCo.,Philadephia.

3 N.Gurumani. (2006). Research Methodology for biological sciences- 1st Edition – MJP
Publishers .
4 Wilson K, and Walker J (2010). Principles and Techniques of Biochemistry and
Molecular Biology.7thEdition. Cambridge University Press .
5 Webster, J.G. (2004). Bioinstrumentation- 4th Edition - John Wiley & Sons (Asia)
Pvt.Ltd,Singapore.

Web Resources
1 http://www.biologydiscussion.com/biochemistry/centrifugation/centrifugeintroduction-
types- uses-and-other-details-with-diagram/12489

2 https://www.watelectrical.com/biosensors-types-its-working-andapplications/

3 http://www.wikiscales.com/articles/electronic-analytical-balance/ Page 24 of 75
 41

4 https://study.com/academy/lesson/what-is-chromatography-definition-typesuses.html

5 http://www.rsc.org/learn-chemistry/collections/spectroscopy/introduction

Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 L M S

CO2 L M S

CO3 L M S

CO4 S S S

CO5 M S
 42

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CI Exter Total
s A nal
22MBUGS Nutrition & Skill Y - - - 2 2 25 75 100
EC2 Health Hygiene Enhance
ment
Course -
SEC-2
(NME)
Course Objectives
CO1 Learn about nutrition and their importance

CO2 Make student understand thenutritional facts fora better life.

CO3 Learn information to optimize our diet

CO4 Impart knowledge on different health care programs taken up by India

CO5 Learn knowledge on different health indicators and types of hygiene methods

Unit Details No.of Course

Hour Objectives
s
I Nutrition – definition, importance, Good nutrition, and mal nutrition; 5 CO1
Balanced Diet: Basics of Meal Planning. Carbohydrates, Lipids,
Proteins and Vitamins –functions, dietary sources, effects of
deficiency. Macro and micro minerals –functions, effects of
deficiency; food sources of Calcium, Potassium, and Sodium; food
sources of Iron, Iodine, and Zinc. Importance of water– functions,
sources, requirements and effects of deficiency
II Nutrition for Life Cycle: Balanced diet - Normal, Pregnant, lactating 5 CO2
women, Infancy, young children Adolescents, Adults, and the Elderly;
Diet Chart; Nutritive value of Indian foods.
III Improper diets: Definition, Identification, Signs and Symptoms - 5 CO3
 43

malnutrition, under-nutrition, over-nutrition, Protein Energy

Malnutrition, obesity; Nutritional Disease and Disorder - hypertension,
diabetes, anemia, osteomalacia, cardiovascular disease.
IV Health - Determinants of health, Key Health Indicators, Environment 5 CO4
health & Public health; Health-Education: Principles and Strategies.
Health Policy & Health Organizations: Health Indicators and National
Health Policy of Govt. of India; Functioning of various nutrition and
health organizations in India.
V Hygiene – Definition; Personal, Community, Medical and Culinary 5 CO5
hygiene; WASH (Water, Sanitation and Hygiene) programme. Rural
Community Health: Village health sanitation & Nutritional committee.
Community & Personal Hygiene: Environmental Sanitation and
Sanitation in Public places.
Total 25
Course Outcomes

Course On completion of this course, students will;

Outcome
s
CO1 Learn the importance of nutrition for a healthy life PO5, PO6, PO7,
PO8, PO10
CO2 Study the nutrition for life cycle PO5, PO6, PO7,
PO8, PO10
CO3 Know the health care programmes of India PO5, PO6, PO7,
PO8, PO10
CO4 Learn the importance of community and personal health & hygiene PO5, PO6, PO7,
measures PO10
CO5 Create awareness on community health and hygiene PO5, PO6, PO7,
PO10
 44

Text Books
1. Bamji, M.S., K. Krishnaswamy& G.N.V. Brahmam (2009) Textbook of Human
Nutrition(3rd edition) Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi
2. Swaminathan (1995)Food &Nutrition(Vol I, Second Edition) The Bangalore Printing
&Publishing Co Ltd., , Bangalore
3 SK. Haldar(2022). Occupational Health and Hygiene in Industry. CBS Publishers.
4 Acharya, Sankar Kr, Rama Das, Minati Sen (2021). Health Hygiene and Nutrition Perception
and Practices.Satish Serial Publishing House
5 Dass (2021).Public Health and Hygiene, Notion Press
References Books

1 VijayaKhader (2000)Food, nutrition & health, Kalyan Publishers, New Delhi

2 Srilakshmi, B., (2010)Food Science, (5th Edition) New Age International Ltd., New Delhi

3 Arvind Kumar Goel (2005). A College Textbook of Health & Hygiene,ABD Publishers
4 Sharma D. (2015).Textbook on Food Science and Human Nutrition.Daya Publishing
House.
5 Revilla M. K. F., Titchenal A. and Draper J. (2020).Human Nutrition.
University of Hawaii, Mānoa.
Web Resources
1 National Rural Health Scheme:
https://nhm.gov.in/index1.php?lang=1&level=1&sublinkid=969&lid=49

2 National Urban Health Scheme:

https://nhm.gov.in/index1.php?lang=1&level=1&sublinkid=970&lid=137

3 Village health sanitation & Nutritional committee

https://nhm.gov.in/index1.php?lang=1&level=1&sublinkid=149&lid=225

4 Health Impact Assessment - https://www.who.int/hia/about/faq/en/

5 Healthy Living https://www.nhp.gov.in/healthylivingViewall

Methods of Evaluation
Continuous Internal Assessment Test 25 Marks
Internal Assignments
Evaluation Seminars
Attendance and Class Participation
External End Semester Examination 75 Marks
 45

Evaluation
Total 100 Marks
Methods of Assessment

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate
between various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons

Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or

Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S M M M S
CO2 S M M M S
CO3 S M M M S
CO4 S S L S
CO5 S S M S

Subject Subject Category L T P S Cre Inst. Marks

Code Name dits Hour CI Exter Total
s A nal
22MBUGSE SERICULT Skill Y - - - 2 2 25 75 100
C3 URE Enhanceme
nt Course -
SEC-3
 46

Course Objectives

CO1 Acquire knowledge on the concepts of origin, growth and study of Sericulture as science
and scientific approach of mulberry plant.
CO2 Describe the morphology and physiology of silkworm.

CO3 Discuss effective management of silkworm diseases.

CO4 Demonstrate field skills in mulberry cultivation and silkworm rearing with an emphasis
on technological aspects.

CO5 Demonstrate entrepreneurship abilities, innovative thinking, planning, and setting up

small-scale enterprises.

Unit Details No.of Course

Hours Objectives
I General introduction to Sericulture, its distribution in India.
Botanical distribution and taxonomical characters of mulberry
5 CO1
varieties and species.Biology of Mulberry plant and Mulberry
crop cultivation and protection.
II Silkworm- biology-morphology of silkworm. Life cycle of
silkworm- egg, larva, pupa, and moth. 5 CO2

III Silkworm pathology: Introduction to Parasitism, Commensalism,

Symbiosis and Parasite relationship - Mulberry Silkworm
Diseases: Introduction, types, Pebrine, Grasserie, Muscardine,
Flacherie, Symptoms and Pathogens, Mode of Infection,
5 CO3
Prevention and Control -Non – mulberry silkworm diseases:
Pebrine, Bacterial and viral diseases. Brief Account of Pests and
Predators of Silkworms, Nature of damage and control
measures.
IV Rearing of silkworm. Cocoon assessment and processing
5 CO4
technologies. Value added products of mulberry and silkworms.
V Entrepreneurship and rural development in sericulture:Planning
for EDP, Project formulation, Marketing, Insectary facilities and
equipments: Location, building specification, air conditioning 5 CO5
and environmental control, furnishings and equipment,
sanitation and equipment, subsidiary facilities.
Total 25
 47

Course Outcomes

Course On completion of this course, students will;

Outcomes

CO1 Discuss the overall aspects of Sericulture and the biology and PO1,PO5,PO7
varieties of mulberry plant.Creates awareness among students
about the economic importance and suitability of Sericulture in
Indian conditions.

CO2 Familiarize with the lifecycle of silk worm. PO1, PO2

Explain common diseases of silkworm encountered during
CO3 rearing, sources of infection, disease symptoms, pre-disposing PO1, PO5
factors and their management practices.
Attain thorough knowledge about the cultivation of mulberry,
CO4 maintenance of the farm, seed technology, silkworm rearing, PO7, PO8, PO10
post cocoon techniques like stifling, reeling, and utilization of
by-products.
Plan the facilities required for establishment of insectary.
CO5 Competent to transfer the knowledge and technical skills to the PO5, PO7, PO8
Seri-farmers.Analyze the importance of sericulture in
entrepreneurship development and emerge as potential
entrepreneur.
Text Books

1 Ganga, G. and Sulochana Chetty (2010). Introduction to Sericulture,, J., Oxford and IBH
Pub. Co. Pvt. Ltd., New Delhi.
2 Dr. R. K. Rajan&Dr. M. T. Himantharaj(2005). Silkworm Rearing Technology, Central
Silk Board, Bangalore.
3 Dandin S B, Jayant Jayaswal and Giridhar K (2010). Handbook of Sericulture
technologies,Central Silk Board, Bangalore.
4 M. C. Devaiah, K. C. Narayanaswamy and V. G. Maribashetty(2010). Advances in
Mulberry Sericulture,,CVG Publications, Bangalore
5 T.V.SatheandJadhav.A.D.(2021). Sericulture and Pest Management, Daya Publishing
House.
References Books

1 S. Morohoshi (2001). Development Physiology of Silkworms 2ndEdition, Oxford & IBH

Publishing Co. Pvt. Ltd. New Delhi
2 Hamamura, Y (2001). Silkworm rearing on Artificial Diet. Oxford & IBH publishing
 48

Co., Pvt. Ltd. NewDelhi.

3 M.Johnson, M.Kesary (2019).Sericulture, 5th.Edition.Saras Publications.
4 Manisha Bhattacharyya (2019).Economics of Sericulture, Rajesh Publications.
5 Muzafar Ahmad Bhat, Suraksha Chanotra, Zafar Iqbal Buhroo, Abdul Aziz and
Mohd.Azam (2020).A Textbook on Entrepreneurship Development Programme in
Sericulture, IP Innovative Publication.
Web Resources

1 https://egyankosh.ac.in › bitstream
2 https://archive.org › details › SericultureHandbook
3 https://www.academic.oup.com
4 https://www.sericulture.karnataka.gov.in
5 https://www.silks.csb.gov.in
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S
 49

CO2 M S
CO3 S S
CO4 S S S
CO5 S S S

SEMESTER III

Subject Subject Name Category L T P S Credits Inst. Marks

Code Hours
CIA External Total
22MBUGCT Molecular Biology Core 4 1 - - 4 5 25 75 100
3 and Microbial Course V
Genetics -Theory
Learning Objectives
CO1 Provide knowledge on structure and replication of DNA.
CO2 Illustrate the significance and functions of RNA in protein synthesis.
CO3 Explain the cause and types of DNA mutation and DNA repair mechanisms.
CO4 Outline the role of plasmids and phages in genetics.
CO5 Examine mechanisms of gene transfer and recombination.
Unit Details No. of Course
Hours Objectives
I DNA Structure - Salient features of double helix, forms of DNA. 15 CO1
Denaturation and renaturation. DNA topology – Supercoiling,
linking number, topoisomerases. DNA organization in
prokaryotes, viruses, eukaryotes. Replication of DNA in
prokaryotes and eukaryotes - Bidirectional and unidirectional
replication, semi-conservative and semi-discontinuous
replication. Mechanism of DNA replication – enzymes involved
– DNA polymerases, DNA ligase, primase. DNA replication
modes - rolling circle, D-loop modes.
II Transcription in Prokaryotes. Concept of transcription. RNA 15 CO2
Polymerases - prokaryotic and eukaryotic. General transcription
factors in eukaryotes. Distinction between transcription
processes in prokaryotes versus eukaryotes. Translation in
prokaryotes and eukaryotes - Translational machinery -
 50

ribosome structure in prokaryotes and eukaryotes, tRNA

structure and processing. Inhibitors of protein synthesis in
prokaryotes and eukaryotes. Overview of regulation of gene
expression - lac, trp and ara operons as examples. Regulation of
gene expression by DNA methylation.
III Mutation - Definition and types - base substitutions, frame 15 CO3
shifts, deletions, insertions, duplications, inversions. Silent,
conditional, and lethal mutations. Physical and chemical
mutagens. Reversion and suppression. Uses of mutations. Repair
Mechanisms - Photoreactivation, Nucleotide Repair, Base
Excision Repair, Methyl Directed Mismatch Repair and SOS
Repair.
IV Plasmid replication and partitioning, host range, plasmid 15 CO4
incompatibility, plasmid amplification, regulation of plasmid
copy number, curing of plasmids. Types of plasmids – R
Plasmids, F plasmids, colicinogenic plasmids, metal resistance
plasmids, Ti plasmid, linear plasmids, yeast 2µ plasmid.
Bacteriophage-T4, Virulent Phage – Structure and lifecycle.
Lambda phage-Structure, Lytic and Lysogenic cycle.
Applications of Phages in Microbial Genetics.
V Gene Transfer Mechanisms- Conjugation and its uses. 15 CO5
Transduction - Generalized and Specialized, Transformation -
Natural Competence and Transformation. Transposition and
Types of Transposition reactions. Mechanism of transposition:
Replicative and non- replicative transposition. Transposable
elements - Prokaryotic transposable elements – insertion
sequences, composite, and non-composite transposons. Uses of
transposons.
Total 75
 51

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Analyze the significance of DNA and elucidate the PO4, PO5, PO7,PO9
replication mechanism.
CO2 Illustrate the types of RNA and protein synthesis PO4, PO7,PO9
machinery.
CO3 Infer the causes and types of DNA mutation and PO5, PO7,PO9
summarize the DNA repair mechanisms.
CO4 Evaluate the importance of plasmids and phages in PO7,PO9
genetics.
CO5 Analyze gene transfer and recombination methods. PO5, PO6, PO7,PO9
Text Books
1. Malacinski G.M. (2008). Freifelder’s Essentials of Molecular Biology. 4th Edition.
Narosa Publishing House, New Delhi.
2. Gardner E. J. Simmons M. J. and SnustedD.P.(2006). Principles of Genetics. 8th
Edition. Wiley India Pvt. Ltd.
3. Trun N. and Trempy J. (2009). Fundamental Bacterial Genetics. 1st Edition. Blackwell
Science Ltd.
4. Brown T. A. (2016). Gene Cloning and DNA Analysis- An Introduction. (7th Edition).
John Wiley and Sons, Ltd.
5. Dale J. W., Schantz M.V. and Plant N. (2012). From Gene to Genomes – Concepts and
Applications of DNA Technology. (3rd Edition). John Wileys and Sons Ltd.
References Books
1. Glick B. R. and Patten C.L. (2018). Molecular Biotechnology – Principles and
Applications of Recombinant DNA. 5th Edition. ASM Press.
2. Russell P.J. (2010). iGenetics - A Molecular Approach, 3rd Edition., Pearson New
International edn.
3. Nelson, D.L. and Cox, M.M. Lehninger(2017). Principles of Biochemistry. 7 th Edition,
W.H. Freeman.
4. Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics of
Bacteria, 4th Edition, ASM Press Washington-D.C. ASM Press.
5. Primrose S.B. and Twyman R. M. (2006). Principles of Gene Manipulation and
Genomics. (7th Edition). Blackwell Publishing
Web Resources
1. [PDF] Lehninger Principles of Biochemistry (8th Edition) By David L. Nelson and
Michael M. Cox Book Free Download - StudyMaterialz.in
2. https://microbenotes.com/gene-cloning-requirements-principle-steps-applications/
3. https://courses.lumenlearning.com/boundless-biology/chapter/dna-replication/
 52

4. Molecular Biology Notes - Microbe Notes

5. Molecular Biology Lecture Notes & Study Materials | Easy Biology Class
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S M S M S M
CO2 S M M S M S L
CO3 M S M S M S L
CO4 M M M S M S L
CO5 M S S S M S L

Subject Subject Name Category L T P S Credits Inst. Marks

Code Hours
CIA Extern Total
al
22MBU Molecular Biology Core - - Y - 4 5 40 60 100
GCP3 Course –VI
and Microbial
– Practical
Genetics III
 53

Learning Objectives

CO1 Provide knowledge on structure and replication of DNA.

CO2 Elucidate the methods of Genomic and Plasmid DNA isolation.
CO3 Explain methods of protein separation.
CO4 Explain artificial transformation method.
CO5 Outline the role of phages in genetics.
Unit Details No. of Course
Hours Objecti
ves
I Study of different types of DNA and RNA using micrographs 15 CO1
and model / schematic representations.
Study of semi-conservative replication of DNA through
micrographs / schematic representations.
II Isolation of Genomic and Plasmid DNA from E. coli and 15 CO2
Analysis by Agarose gel electrophoresis.
Estimation of DNA using colorimeter (diphenylamine reagent),
UV spectrophotometer (A260 measurement).
III Resolution and visualization of proteins by polyacrylamide gel 15 CO3
electrophoresis (SDS-PAGE) – Demonstration.
UV induced auxotrophic mutant production and isolation of
mutants by replica plating technique – Demonstration.
IV Perform artificial Transformation in E. coli. 15 CO4
Isolation of antibiotic resistant mutants by gradient plate method.
- Demonstration
V Screening and isolation of phages from sewage. 15 CO5
Perform RNA isolation.
Estimate RNA.
Total 75
 54

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Illustrate different types of DNA and RNA. PO4, PO7, PO9, PO11
CO2 Utilize hands-on training in isolation of genomic and PO4, PO7, PO9, PO11
plasmid DNA.
CO3 Analyze importance of experimental microbial genetics. PO4, PO7, PO9, PO11
CO4 Apply the knowledge of molecular techniques in various PO4, PO7, PO9, PO11
fields.
CO5 Investigate the significance of Phages. PO4, PO7, PO9, PO11

Text Books

1. Crichton. M. (2014). Essentials of Biotechnology. Scientific International Pvt

Ltd.New Delhi.
2. Sambrook J. and Russell D.W. (2001). Molecular Cloning - A Laboratory Manual –
7th Edition. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press.
3. Dale J. W., Schantz M. V. and Plant N. (2012). From Gene to Genomes – Concepts
and Applications of DNA Technology. (3rd Edition). John Wileys and Sons Ltd.
4. Gunasekaran P. (2007). Laboratory Manual in Microbiology. New Age International.
5. James G Cappucino. and Natalie Sherman. (2016). Microbiology – A laboratory
manual. (5th Edition). The Benjamin publishing company. New York.
References Books
1 Glick B. R. and Patten C.L. Molecular Biotechnology – Principles and Applications
of Recombinant DNA. 5th Edition. ASM Press. 2018.
2 Russell P.J. (2010). iGenetics - A Molecular Approach, 3 rd Edition., Pearson New
International edn.
3 Nelson, D.L. and Cox, M.M. Lehninger(2017). Principles of Biochemistry. 7 th
Edition, W.H. Freeman.
4 Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics
of Bacteria, 4th edition, ASM Press Washington-D.C. ASM Press.
5 Brown T.A. (2016). Gene Cloning and DNA Analysis. (7th Edition). John Wiley and
Jones, Ltd.
Web Resources
1 https://www.molbiotools.com/usefullinks.html
2 (PDF) Molecular Biology Laboratory manual (researchgate.net)
3 https://www.molbiotools.com/usefullinks.html
4 https://geneticgenie.org3.
5 https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpet.5
Methods of Evaluation
Continuous Internal Assessment Test
Internal
Assignments 25 Marks
Evaluation
Seminars
 55

Attendance and Class Participation

External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks

Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S L M S M S M S
CO2 S L M S M S M S
CO3 S L M S M S M S
CO4 S L M S M S M S
CO5 S L M S M S M S
 56

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CIA Exte Total
s rnal
 57

22MBUGD CLINICAL ELECTIVE Y - - - 3 4 25 75 100

E3 GENERIC/D
LABORATOR
ISCIPLINE
Y SPECIFIC
ELECTIVE
TECHNOLOG
-III
Y

Learning Objectives

CO1 Demonstrate ethical and professional conduct with patients, laboratory personnel, health-
care professionals, and the public.
CO2 Explain how accurate and reliable information might be obtained about proper procurement,
storage, and handling of laboratory specimens.
CO3 Develop a sound scientific knowledge foundation that prepares them to interpret, analyze
and evaluate scientific knowledge in clinical practice.
CO4 Perform a full range of laboratory tests with accuracy and precision.
CO5 Establish quality assurance principles and practices to ensure the accuracy and reliability of
laboratory information.
Unit Details No.of Course
Hours Objectives
I Introduction to Clinical Laboratory Science: Basic laboratory 12 CO1
principles - Code of conduct for medical laboratory personnel -
Organization of clinical laboratory and role of medical laboratory
technician - Safety measures. Assessment of a patient and brief
history of collection. Maintenance of Hygiene & Infection Control
Practices.
II Specimen collection and processing - Blood, urine, stool, sputum 12 CO2
CSF, amniotic fluid and bile. Separation of serum and plasma,
Handling of specimens for testing, preservation of specimens,
transport of specimens and factors affecting the clinical results.

III Introduction to histopathology-Methods of examination of tissues 12 CO3

and cells, Fixation of tissues: Classification and properties of
 58

fixatives. Tissue processing - Collection of specimens, Labeling and

fixation, Dehydration, Clearing, Impregnation, Embedding - Paraffin
block making, Section Cutting, Microtomes – types and mounting of
sections.
IV Introduction to Haematology- Laboratory methods used in the 12 CO4
investigation of coagulation disorders - coagulation tests , Routine
coagulation tests, (prothrombin time , plasma recalcification
time,partial thromboplastin time , activated partial thromboplastin
time, thrombin time), Laboratory diagnosis of bleeding disorders.
Estimation of fibrinogen, Assay of coagulation factors.
V Quality Standards in Health Laboratories – Development and 12 CO5
implementation of standards, Accreditation Boards –NABL, ISO,
CAP, COLA, Performing quality assessment - pre-analytical,
analytical, and post-analytical phases of testing.
Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Describe characteristics of laboratory organizations and demonstrate PO3, PO11
professionalism by displaying professional conduct, model ethical
behavior and operate as a vital member of the medical lab team.
Practice safety or infection control procedures in the clinical
laboratory, properly use safety equipment and maintain a clean, safe
work environment.
CO2 Accurately collect specimens for various purposes. Determine PO5, PO6, PO11
appropriate tests based on test request, Maintain standard and
transmission-based precautions, Engage in the scientific process
by understanding the principles and practices of clinical study
 59

design, implementation, and dissemination of results.

CO3 Identify the basic structure of cells, tissues and organs and describe PO6, PO8, PO9,
PO11
their contribution to normal function. Interpret light and electron
microscopic histological images and identify the tissue source and
structures. Relate and recognize the histological appearance of
affected tissues to the underlying pathology.

CO4 Recognize the pathologies behind benign and malignant disorders of PO5, PO6, PO9,
PO11
erythrocytes, leucocytes, thrombocytes and familiar with the
diagnosis, evaluation, and management of hematologic malignancies.
CO5 Interpret, implement, and complying with laws, regulations and PO1,PO10
accrediting standards and guidelines of relevant governmental and
non-governmental agencies.
Text Books

1. Mukharji,K.L. (2000).Medical Laboratory Techniques, Vol - I, II & III, 5 th Edition. Tata

McGrawHill, Delhi.

2. Ochei,A., Kolhatkar.A. (2000).Medical Laboratory Science: Theory and Practice,

McGraw Hill Education.

3 RamnikSood (2015).Concise Book of Medical Laboratory Technology:Methods and

Interpretation, 2ndEdition, Jaypee Brothers Medical Publishers, NewDelhi.

4. S. Ramakrishnan, KN Sulochana(2012). Manual of Medical Laboratory

Techniques,Jaypee Brothers Medical Publishers Pvt. Ltd
5. Talib V.H. (2019).Handbook Medical Laboratory Technology, 2ndEdition, Directorate
of health services, Government of India.
References Books

1 Rutherford, B.H. Gradwohl , A.C. Sonnenwirth L. Jarett. Gradwohls. (2000). Clinical

Laboratory Methods and Diagnosis, Vol-I, 8th edition, Mosby.
2 Baker, F.J., Silverton, R.E., and Pallister,.J. (1998). An Introduction to Medical
Laboratory Technology, 7thEdition, CBS Publishers and Distributors Pvt. Ltd.
3 Godkar (2021).Textbook of Medical Laboratory Technology, 3rdEdition,Bhalani
Publishing House.
4 M.N.Chatterjee and RanaShinde.(2008). Textbook of Medical Biochemistry, 7 thEdition,
Jaypee Brothers Medical Publishers Pvt. Limited.
5 James G Cappucino. and Natalie Sherman. (2016). Microbiology – A laboratory
 60

manual.(5th Edition).The Benjamin publishing company. New York.

Web Resources

1 https://www.jaypeedigital.com › book
2 https://www.pdfdrive.com › wintrobes-clinical-hematology
3 https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpet.5
4 https://vlab.amrita.edu/index.php?sub=3&brch=272
5 https://nptel.ac.in/courses/102105087
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 M S
CO2 M S S
CO3 S S S S
CO4 M S S S
CO5 M M
Subject Subject Name Category L T P S Cred Inst. Marks
Code its Hours
CIA Exter Total
nal
 61

22MBUGS ORGANIC SKILL Y - - - 1 1 25 75 100

EC4 FARMING & ENHANC
BIOFERTILISER EMENT
TECHNOLOGY COURSE
– SEC -4
(ENTREP
RENEUR
IAL
SKILL)
Learning Objectives

CO1 Impart knowledge about the significance of organic farming and strategies to increase
the yield to conserve environment.
CO2 To encourage organic farming in urban areas.
CO3 Comprehensive knowledge about bacterial biofertilizers, its advantages and future
perspective.
CO4 Structure and characteristic featuresof Cyanobacterial and fungal biofertilizer
CO5 Develop the knowledge and skill to produce, analyze the quality of packaging, storage
and assess the shelf life and bioefficacy of biofertilizers.
Unit Details No.of Course
Hours Objectiv
es
I Principle of organic farming: principles of health, fairness, 6 CO1
ecological balance, and care.Environmental benefits of organic
farming: sustainability- reduces non-renewable energy by
decreasing agrochemical need. Biodiversity-crop rotation, inter-
cropping. Ecological services – biological control, soil formation
and nutrient cycling.
II Organic farming for urban space; Create a Sustainable Organic 6 CO2
Garden (Backyard- Square Foot Gardening, Small Space
Gardening, Mini Farming) Composting, Vermicomposting
III Biofertilizers: Introduction, advantages and future perspective. 6 CO3
Structure and characteristic features of bacterial biofertilizers-
Azospirillum, Azotobacter, Bacillus, Pseudomonas, Rhizobium and
Frankia
IV Structure and characteristic features 6 CO4
ofCyanobacterialbiofertilizers- Anabaena, Nostoc ;Structure and
characteristic features offungal biofertilizers- AM mycorrhiza
 62

V Production of Rhizobium, Azotobacter, Anabena;Biofertilizers - 6 CO5

Storage, shelf life, quality control and marketing
Total 30

Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Become an Entrepreneur with wide knowledge about farming and PO1, PO2, PO7,
sustainable resources. PO8, PO10
CO2 Implement organic farming in urban areas with knowledge on PO1, PO5, PO10
compost.
CO3 PO1, PO5, PO7,
Gain knowledge about the bacterial biofertilizers and its PO8, PO10
advantages
CO4 PO1, PO5, PO7,
Understand the significance about Cyanobacterial and fungal PO8, PO10
biofertilizers
CO5 PO1, PO5, PO7,
Understand and implement the use of bio fertilizers. PO8, PO10
Text Books

1. A.K. Sharma (2006). Hand book of Organic Farming

2. A.C.Gaur (2017). Hand book of Organic Farming and Biofertilizers

3. N.S. Subbarao (2017). Bio-fertilizers in Agriculture and Forestry (4 th Edition) Med

tech publisher

4. SubbaRao, N. S. (2002). Soil Microbiology. Soil Microorganisms and Plant Growth.

(4th Edition), Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.

5. Dubey, R. C. (2008). A Textbook of Biotechnology. S. Chand & Co., New Delhi.

References Books
1 Masanobu Fukuoka, Frances Moore Lappe Wendell Berry (2009). The One-Straw
Revolution: An Introduction to Natural Farming, 1st edition, YRB Classics.
2 SujitChakrabarty(2018). Organic Home Gardening Made Easy, 1st Edition,
3 Singh and Purohit (2008). Biofertilizer technology. Agrobios, India.
4 Bansal M (2019). Basics of Organic Farming CBS Publisher.
5 Hurst, C.J., Crawford R.L., Garland J.L., Lipson D.A., Mills A.L. and Stetzenbach
L.D. (2007). Manual of Environmental Microbiology. (3rd Edition). American
Society for Microbiology.
 63

Web Resources

1. https://agritech.tnau.ac.in/org_farm/orgfarm_introduction.html
2. https://www.fao.org/organicag/oa-faq/oa-faq6/en/
3. https://www.india.gov.in/topics/agriculture/organic-farming
4. https://agriculture.nagaland.gov.in/bio-fertilizer/
5. https://vlab.amrita.edu/index.php?sub=3&brch=272
Methods of Evaluation

Continuous Internal Assessment Test

Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment

Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions

Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

CO/PO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S S S
CO2 S S S
CO3 S S S S S
CO4 S S S S S
CO5 S S S S S

Subject Subject Name Cate L T P S Credit Inst. Marks

 64

Code gory s Hour CI Exter Tota

s A nal l
22MBUGS AQUACULTURE Skill Y - - - 2 2 25 75 100
EC5 Enha
ncem
ent
Cour
se -5
Learning Objectives

CO1 Provide a deeper knowledge in aquaculture systems and methods.

CO2 Explain the significance and functions of design, types and construction of
aquaculture ponds.
CO3 Demonstrate the biological characteristics of various aquaculture species.
CO4 Discuss the methods involved in post stocking management.
CO5 Illustrate major cultivatable species for aquaculture.
Unit Details No. of Course
Hours Objecti
ves
I Aquaculture Systems and Methods - Scope and definition. 6 CO1
Traditional, extensive, semi - intensive and intensive culture.
Monoculture, polyculture, composite culture, mixed culture,
mono-sex culture, cage culture, pen culture, raft culture, race way
culture.
II Aquaculture Engineering - Design and construction of pond, lay- 6 CO2
out and design of aquaculture farm, construction, water intake
system, drainage system - aeration and aerators. Ponds - Types of
ponds.
III Selection of Species - Biological characteristics of aquaculture 6 CO3
species; economic and market considerations; seed resources,
collection and transportation. Pre-Stocking Management-Sun
drying, ploughing / tilling, desilting, liming and fertilization,
eradication of weed fishes. Stocking - Acclimatization of seed
and release - species combinations - stocking density and ratio.
IV Post Stocking Management - Water and soil quality parameters 6 CO4
 65

required for optimum production, control of aquatic weeds and

aquatic insects, algal blooms and microorganisms. Food
conversion ratio (FCR). Growth - Measurement of growth, length
- weight relationship.
V Major cultivable species for aquaculture –Culture of Indian Major 6 CO5
Carps. Culture of Giant fresh water prawn,
Macrobrachiumrosenbergii - seed collection formation sources.
Hatchery management. Culture of tiger shrimp, Penaeusmonodon
and LitopenaeusVannamei. Culture of pearl oysters. Culture of
sea weeds. Methods of Crab culture. Culture of ornamental
fishes. Culture of Molluscs.

Total 30
Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Analyze the significance and importance of aquaculture PO4, PO5,
PO7,PO9
CO2 Illustrate the types and construction of aquaculture ponds PO4, PO7,PO9
CO3 Analyze the biological characteristics of species and choose the PO5, PO7,PO9
best species for aquaculture.
CO4 Follow methods involved for optimal growth of aquaculture PO7,PO9
species
CO5 Summarize major species suitable for aquaculture in a particular PO5, PO6,
PO7,PO9
environment
Text Books
1. Santhanam, R. Velayutham, P. Jegatheesan, G. A (2019).Manual of Freshwater
Ecology: An Aspect of Fishery Environment. Daya Publishing House, New Delhi.
2. Stickney, R.R. (2016). Aquaculture: An Introductory Text. 3rd Edition. Centre for
Agriculture and Bioscience International Publishing.
3. Ackefors H., Huner J and Konikoff M. (2009). Introduction to the General Principles
of Aquaculture. CRC Press.
4. Mushlisin Z. A. (2012). Aquaculture. In Tech.
5. Akpaniteaku R.C. (2018).Basic Handbook of Fisheries and Aquaculture.AkiNik
Publications.

References Books
 66

1. Arumugam N. (2014). Aquaculture. Saras Publication.

2. Pillay T. V. R. and Kutty M.N. (2005). Aquaculture : Principles and Practices.
2ndEdition. Wiley India Pvt. Ltd.
3. Tripathi S. D., Lakra W.S. and Chadha N.K. (2018). Aquaculture in India. Narendra
Publishing House.
4. Rath R.K.(2011). Fresh Water Aquaculture. 3rdEdition. Scientific Publishers.
5. Lucas J. S., Southgate P.C. and Tucker C.S. (2019). Aquaculture: Farming Aquatic
Animals and Plants. Wiley Blackwell.
Web Resources

1. Aquaculture: Types, Benefits and Importance (Fish Farming) - Conserve Energy

Future (conserve-energy-future.com)
2. Fisheries Department - Tamil Nadu (tn.gov.in)
3. Aquaculture - Google Books
4. aquaculture | Definition, Industry, Farming, Benefits, Types, Facts, & Methods |
Britannica
5. Fisheries & Aquaculture (investindia.gov.in)
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

 67

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S M S M S M
CO2 S M M S M S L
CO3 M S M S M S L
CO4 M M M S M S L
CO5 M S S S M S L

SEMESTER VI
Subject Subject Name Category L T P S Credit Inst. Marks
Code s Hours
CIA Exter Total
nal
22MBUGC IMMUNOLOG CORE Y - - - 4 5 25 75 100
T4
Y AND COURSE

IMMUNOTECH – VII

NOLOGY

Course Objectives

CO1 To gain knowledge about immune system, organs of immunity and cells involved.

CO2 To distinguish the types of antigens and antibodies; their properties.

CO3 To provide in-depth knowledge on immuno-techniques.

CO4 To discuss the role of MHC system in transplantation; functions of Tumor specific
antigens.

CO5 To impart knowledge on immunological disorders.

Unit Details No.of Course

Hours Objectives
I Organs and Cells in Immune System and Immune 12 CO1
Response:Primary lymphoid organs, secondary lymphoid organs,
and lymphoid tissues; T – cell and B –cell membrane bound
receptors – apoptosis; T - cell processing, presentation and
regulation; T –cell subpopulation, properties, functions and T –
 68

cell suppression; Physiology of immune response- innate, humoral

and cell mediated immunity; Immunohematology.
II Antigen and Antibody:Antigens - Properties of haptens, epitopes, 12 CO2
adjuvants, and cross reactivity; Antibodies- structure, properties,
classes; Antigen and Antibody Reactions: precipitation,
agglutination, complement fixation, opsonization, neutralization;
Vaccines – active and passive immunization; Classification of
vaccines; Other approaches to new vaccines; Types of vaccine -
antibacterial, antiviral; Vaccination schedule.
III Immunoassay and Immunotechniques - Preparation and 12 CO3
standardization of bacterial antigens; Raising of monoclonal and
polyclonal antibodies; Purification of antibodies.
Immunotechniques - RIA, RAST, ELISA, Immuno fluorescence
techniques and Flow cytometry
IV Transplantation and TumorImmunology - MHC Antigens - 12 CO4
structure and function; HLA system - Regulation and response to
immune system; Transplantation immunology - tissue
transplantation and grafting; Mechanism of graft acceptance and
rejection; HLA typing; Tumor specific antigens; Immune response
to tumors; Immune diagnosis; cancer immune therapy.
V Immunological disorders and diseases - Hypersensitivity reactions 12 CO5
(Type I, II, III and IV); acquired immunodeficiency syndrome;
Auto immune disorders and diseases: organ specific and non-
organ specific.

Total 60
Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Assess the fundamental concepts of immunity, contributions of the PO1, PO4, PO6,
PO9,
organs and cells in immune responses.
CO2 Investigate the structures of Ag and Ab; Immunization. PO1, PO4, PO5, PO9
 69

CO3 Justify the Immunoassay and Immunotechniques. PO1, PO4, PO5, PO7
CO4 Explain about the immunologic processes governing graft PO1, PO3, PO4,
PO5, PO9
rejection and therapeutic modalities for immunosuppression in
transplantation
CO5 Analyze the overreaction by our immune system leading to PO1, PO4, PO5, PO6
hypersensitive conditions and its consequences.

Text Books
1. Richard Coico, Geoffrey Sunshine, Eli Benjamini. (2003). Immunology – A Short
Course. 5thEdition., Wiley-Blackwell, New York.

2. Judith A.Owen, Jenni Punt, Sharon A. Stranford, Janis Kuby. (2013). Immunology,
7thEdition., W. H. Freeman and Company, New York.

3. Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai. (2021). Cellular and Molecular
Immunology, 10thEdition.,Elsevier.

4. Robert R. Rich, Thomas A. Fleisher, William T. Shearer, Harry Schroeder, Anthony J.

Frew, Cornelia M. Weyand. (2018).Clinical Immunology: Principles and Practice, 5 th
Edition. Elsevier.

5. Pravash Sen. Gupta. (2003). Clinical Immunology. Oxford University Press.

References Books
1 Janeway Travers. (1997). Immunobiology- the immune system in health and disease.
Current Biology Ltd. London, New York. 3rd Edition.
2 Peter J. Delves, Seamus Martin, Dennis R. Burton, Ivan M. Roitt. (2006). Roitt’s
Essential Immunology, 11thEdition.,Wiley-Blackwell.

3 William R Clark. (1991). The Experimental Foundations of Modern Immunology.

3rdEdition. John Wiley and Sons Inc. New York.
4 Frank C. Hay, Olwyn M. R. Westwood. (2002). Practical Immunology, 4thEdition.,
Wiley-Blackwell.

5 Noel R. Rose, Herman Friedman, John L. Fahey. (1986). Manual of Clinical

Laboratory Immunology. ASM.3rd Edition.
Web Resources
1 https://www.ncbi.nlm.nih.gov/books/NBK279395/
2 https://med.stanford.edu/immunol/phd-program/ebook.html
 70

3 https://ocw.mit.edu/courses/hst-176-cellular-and-molecular-immunology-fall-2005/pages/
lecture-notes/
4 Immunology Overview - Medical Microbiology - NCBI Bookshelf (nih.gov)

5 Immunology - an overview | ScienceDirect Topics

Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9

CO1 S M S M
CO2 S M M M
CO3 S S S S
CO4 S M S S M
CO5 S S M M
 71

Subject Subject Name Categor L T P S Cre Inst. Marks

Code y dits Hou
CIA Exter Total
rs
nal
 72

22MBU IMMUNOLOGY - - Y - 4 5 40 60 100

GCP4 CORE
AND COUR
IMMUNOTECHNOL SE –
VIII-
OGY PRAC
TICAL
IV

Course Objectives

CO1 To gain hands-on knowledge to identify Blood group and typing.

CO2 To acquire adequate skill to perform latex agglutination reactions.

CO3 To analyze precipitation reactions in gels.

CO4 To investigate the antigen & antibody reactions in electrophoresis.

CO5 To familiarize with Separation of Lymphocytes.

Unit Details No.of Course

Hours Objectives
I Identification of blood group and typing. 12 CO1
Coomb’s test. TPHA
II T cell identification (Demonstration) 12 CO2
Latex Agglutination reactions- RF, ASO, CRP
III Ouchterlony’s Double Diffusion Method (antigen pattern). 12 CO3
Single Radial Immuno Diffusion Method.
IV Electrophoresis - Serum, Counter and Immuno. 12 CO4
V Separation of Lymphocytes by gradient centrifugation method. 12 CO5
ELISA: Hepatitis/ HIV
Total 60
Course Outcomes

Course On completion of this course, students will;

Outcome
s

CO1 Assess the blood groups and types PO1,PO5, PO6, PO7, PO8
CO2 Competently perform serological diagnostic tests such as PO4, PO5, PO6, PO7, PO8
RF, ASO, CRP
 73

CO3 Illustrate the antigen antibody reactions in gel. PO5, PO6, PO7, PO8, PO9
CO4 Compare & contrast antigens and antibodies in PO5, PO6, PO7, PO8, PO9
electrophoresis
CO5 Examine the concept of ELISA. PO5, PO6, PO7, PO8, PO9
Text Books

1. Talwar. (2006). Hand Book of Practical and Clinical Immunology, Vol. I, 2nd
edition, CBS.
2. Asim Kumar Roy. (2019). Immunology Theory and Practical, Kalyani Publications.
3. Richard Coico, Geoffrey Sunshine, Eli Benjamini. (2003). Immunology – A Short
Course. 5thEdition., Wiley-Blackwell, New York.

4. Judith A.Owen, Jenni Punt, Sharon A. Stranford, Janis Kuby. (2013). Immunology,
7thEdition., W. H. Freeman and Company, New York.

5. Pravash Sen. Gupta. (2003). Clinical Immunology. Oxford University Press.

References Books
1 Frank C. Hay, Olwyn M. R. Westwood. (2008).Practical Immunology, 4th Edition,
Wiley-Blackwell.

2 Wilmore Webley. (2016). Immunology Lab Manual, LAD Custom Publishing.

3 Rose. (1992). Manual of Clinical Lab Immunology, ASM.

4 Janeway Travers. (1997). Immunobiology- the immune system in health and disease.
Current Biology Ltd. London, New York. 3rd Edition.
5 Peter J. Delves, Seamus Martin, Dennis R. Burton, Ivan M. Roitt. (2006). Roitt’s
Essential Immunology, 11thEdition.,Wiley-Blackwell.

Web Resources
1 https://www.researchgate.net/publication/275045725_Practical_Immunology-
_A_Laboratory_Manual
2 https://www.urmc.rochester.edu/MediaLibraries/URMCMedia/labs/frelinger-lab/
documents/Immunology-Lab-Manual.pdf
3 https://webstor.srmist.edu.in/web_assets/downloads/2021/18BTC106J-lab-manual.pdf
4 Immunology Overview - Medical Microbiology - NCBI Bookshelf (nih.gov)

5 Immunology - an overview | ScienceDirect Topics

Methods of Evaluation
Internal Continuous Internal Assessment Test 25 Marks
 74

Assignments
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9

CO1 M S S S S
CO2 S M M S S
CO3 M S S S M
CO4 M M S S M
CO5 M M S S M
 75

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hours
CIA Exter Total
nal
22MBU FOOD ELECTIV Y - - - 3 4 25 75 100
GDE4 E
PROCESSING GENERIC/
TECHNOLOGY DISCIPLI
NE
SPECIFIC
ELECTIV
E -IV

Learning Objectives
CO1 To provide knowledge on objectives of food preservation.
CO2 To explain the freshness criteria and quality assessment of meat and fish.
CO3 To outline the methods of milk processing and fermented milk products.
CO4 To explain the importance of fat and oil processing.

CO5 To discuss the methods of microbiological examination of foods.

Unit Details No.of Course

Hours Objectives
I Introduction to food preservation –objectives and techniques of food 12 CO1
preservation. Preservation: principles of high temperature, low
temperature, radiation, chemical preservatives and bio preservatives.
II Freshness criteria and quality assessment of meat and fish –spoilage 12 CO2
and methods of preservation. Production of byproducts after
processing waste and their utilization. Role of packaging material,
types of packaging material.
III Composition of milk; assessment of milk, thermal processing of 12 CO3
fluid milk-pasteurization (LTH, HTST&UHT techniques).
Fermented milk products-cheese, Butter milk, Yogurt, Kumis, Kefir
and Acidophilus milk. Hygiene and sanitation requirement in food
processing and fermentation industries.
IV Importance of fats and oils in Food-Extraction of fats and Oils- 12 CO4
Rendering, pressing, solvent extraction, pressing of oil- degumming,
refining, bleaching, deodorization, fractionation, pyrolysis of fats,
toxicity of frying oil.

V Methods for the microbiological examination of foods. Food borne 12 CO5

 76

illness and diseases. Microbial cultures for food fermentation. Indian

Factories Act on safety, HACCP, Safety from adulteration of food.
Total 60
Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Assess the fundamental concepts of food preservation. PO1, PO3, PO5,PO6,
PO8
CO2 Investigate the quality assessment of meat and fish. PO1, PO5, PO6,
PO7, PO8
CO3 Design the processing of milk and milk quality assessment. PO1, PO5, PO6,
PO7, PO8
CO4 Explain about the importance of fats and oils. PO1, PO4, PO6,
PO7, PO8
CO5 Plan the food safety and adulteration detection. PO3, PO4, PO6,
PO7, PO8
Text Books

1. Avantina Sharma. (2006). Text Book of Food Science and Technology, International
Book
Distributing Co, Lucknow, UP.

2. Sivasankar. (2005). Food Processing and Preservation, 3rd Edition.,Prentice hall of

India Pvt Ltd,
NewDelhi.
3 Ramaswamy H & Marcotte M. (2006). Food Processing: Principles & Applications.
Taylor & Francis.

4 NIIR Board of Food and Technologist. (2005). Modern Technology of Food

Processing and Agrobased industries, National Institute of Industrial Research, Delhi.

5 Adams M.R. and Moss M. O (2007).Food Microbiology.New Age International.

Reference Books

1 Fellos PJ. (2005). Food Processing Technology: Principle &Practice 2ndEdition. CRC.

2 Peter Zeuthen and Leif Bogh-Sorenson. (2005). Food Preservation Techniques,

WoodlandPublishing Ltd, Cambridge, England.1

3 Gustavo V. Barbosa-Canovas, Maria S. Tapia, M. Pilar Cano. (2004). Novel Food

Processing Technologies, CRC.
4 Suman Bhatti, Uma Varma. (1995). Fruit and vegetable processing organizations and
 77

institutions, 1st Edition., CBS Publishing, New Delhi.

5 MirdulaMirajkar, Sreelatha Menon. (2002). Food Science and Processing Technology

Vol-2,Commercial processing and packaging, Kanishka publishers, New Delhi.

Web Resources
1 https://sites.google.com/a/uasd.in/ecourse/food-processing-technology
2 https://nptel.ac.in/courses/126105015
3 https://engineeringinterviewquestions.com/biology-notes-on-food-adulteration/
4 food processing | Definition, Purpose, Examples, & Facts | Britannica
5 Food Processing Technology | Food News & Views Updated Daily (foodprocessing-
technology.com)

Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
 78

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9

CO1 M M S M S
CO2 M S M S S
CO3 M S M S S
CO4 M S S S S
CO5 M M M S S

Subjec Subject Name Category L T P S Credi Inst. Marks

t ts Hou CI Extern Total
Code rs A al
22MB Vaccine Skill Y - - - 2 2 25 75 100
UGSE Enhancem
C6 Technology ent Course
SEC -6

Course Objectives

CO1 To provide knowledge on the basics of immunization and induction of immunity.

CO2 To learn the types of vaccines, its immunological effects and regulatory guidelines.
CO3 To learn the role of rDNA in vaccine technology.
CO4 To provide the knowledge on conventional to recent technology of vaccine
production
CO5 To learn about ethical issues and regulations in vaccine production and clinical trials
Unit Details No.of Course
Hours Objectives
I History of vaccination, Active and passive 3hrs CO1
immunization; requirements for induction of immunity,
Epitopes, linear and conformational epitopes,
characterization and location of APC, MHC and
immunogenicity,
II Viral/bacterial/parasite vaccine differences, methods of 6 CO2
vaccine preparation – Live, killed, attenuated, sub unit
vaccines;Licensed vaccines, Viral Vaccine - Poliovirus
 79

vaccine-inactivated & Live, Rabies vaccines, Hepatitis A

& B vaccines, Bacterial Vaccine - Anthrax vaccines,
Cholera vaccines, Diphtheria toxoid, Parasitic vaccine -
Malaria Vaccine.
III Vaccine technology- Role and properties of adjuvants, 5 CO3
recombinant DNA and protein-based vaccines, plant-
based vaccines, reverse vaccinology; Peptide vaccines,
conjugate vaccines. Recent advances in Malaria,
Tuberculosis, HIV.
IV Fundamental research to rational vaccine design. 5 CO4
Antigen identification and delivery, T-Cell expression
cloning for identification of vaccine targets for
intracellular pathogens,Rationale vaccine design based
on clinical requirements: Scope of future vaccine
strategies.
V Vaccine additives and manufacturing residuals, 5 CO5
Regulation and testing of vaccines, Regulation of
vaccines in developing countries, Quality control and
regulations in vaccine research, Animal testing, Rational
design to clinical trials, Large scale production,
Commercialization. Vaccine safety ethics and Legal
issues.
Total 24
Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Explain the significance of critical antigens, PO1,PO10
immunogens and adjuvants in developing effective
vaccines.
CO2 Understand the types of vaccines. PO5
CO3 Construct vaccine applying rDNA technology. PO7,PO10
CO4 Formulate the strategies for developing an innovative PO9,PO10
vaccine technology with different mode of vaccine
delivery.
CO5 Evaluate the regulatory issues and guidelines for the PO3,PO5
 80

management of vaccine production.

Text Books
1. Ronald W. Ellis.(2001). New Vaccine Technologies.Landes Bioscience.
2. Cheryl Barton. (2009). Advances in Vaccine Technology and Delivery.Espicom
Business Intelligence.
3 Male, David. Ed. (2007). Immunology. 7th Edition. Mosby Publication.
4 Kuby, RA Goldsby, Thomas J. Kindt, Barbara, A. Osborne. (2002). Immunology. 6 th
Edition, Freeman.
5 Brostoff J, Seaddin JK, Male D, Roitt IM. (2002). Clinical Immunology. 6 th Edition,
Gower Medical Publishing.
References Books

1 Stanley A. Plotkin, Walter Orenstein& Paul A. Offit.(2013). Vaccines, 6 th Edition.

BMA Medical Book Awards Highly Commended in Public Health. Elsevier
Publication.
2 Coico, R. etal. (2003). Immunology: A Short Course. 5th Edition, Wiley – Liss.
3 Parham, Peter.(2005). The Immune System. 2nd Edition, Garland Science.
4 Abbas, A.K. etal. (2007). The Cellular and Molecular Immunology. 6 th Edition,
Sanders / Elsevier.
5 Weir, D.M. and Stewart, John (2000). Immunology. 8th Edition, Churchill Pvt. Ltd.
Web Resources
1 https://www.slideshare.net/adammbbs/pathogenesis-3-rd-internal-updated-43458567
2 https://www.bio.fiocruz.br/en/images/stories/pdfs/mpti/2013/selecao/vaccine-
processtechnology.pdf
3 https://www.dcvmn.org/IMG/pdf/
ge_healthcare_dcvmn_introduction_to_pd_for_vaccine_
production_29256323aa_10mar2017.pdf
4 https://www.sciencedirect.com/science/article/pii/B9780128021743000059
5 https://www.researchgate.net/publication/
313470959_Vaccine_Scaleup_and_Manufacturing

Methods of Evaluation

Continuous Internal Assessment Test 25 Marks

Internal Assignments
 81

Evaluation Seminars
Attendance and Class Participation
External
Evaluation
End Semester Examination 75 Marks
Total 100 Marks
Methods of Assessment
Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions
Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application
(K3)
Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe, Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between variou
ideas, Map knowledge
Evaluate
(K5)
Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M M
CO2 S
CO3 M M
CO4 L M
CO5 L M

Subject Subject Name Category L T P S Credits Inst. Marks

Code Hours
CIA External Total
22MBU APICULTURE SKILL Y - - - 2 2 25 75 100
GSEC7 ENHANCEMENT
COURSE- SEC – 7

Course Objectives

CO1 To understand the biology of honey bees.

CO2 To study on honey bee colony establishment.

CO3 To develop knowledge on honey extraction.
CO4 To understand the diseases of honey bees and their control.
CO5 To gain information on financial assistance and funding agencies for bee keeping industry.
 82

Unit Details No.of Course

Hours Objectives

I Biology of Bees: Honeybee – Systematic position – Species of 6 CO1

Honey bees – Life history of Honey bee – behaviour – swarming –
Pheromone.

II Social life in Bees:Bee colony – Castes – natural colonies and their 6 CO2
yield – Types of bee hives – Structure – location, care and
management.

III Bee Rearing:Apiary – Care and Management – Artificial bee hives 6 CO3
– types – construction of spaceframes – Selection of sites –
Handling – Maintenance – Instruments employed in Apiary –
Extraction instruments.

IV Bee Economy: Honey – Composition – uses – Bee wax and its uses 6 CO4
– yield in national and international market – Diseases of honey
bees and their control methods. Economics of bee culture.

V Entrepreneurship: venture – Preparing proposals for financial 6 CO5

assistance and funding agencies – Bee Keeping Industry – Recent
Efforts, Modern Methods in employing artificial Beehives for cross
pollination in horticultural gardens.

Total 30

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Understand the systematic position and life history of honey bee. PO1, PO2, PO10

CO2 Reveal the different stages and types of bees and discuss about the PO1, PO2, PO4, PO5
care and management of apiculture.

CO3 Describe the practice of bee rearing process and analyze PO2,PO4, PO5, PO10,
instruments employed in apiary. PO11

CO4 Compare and contrast the composition of honey and bee wax and PO4, PO5, PO7, PO8,
interpret the yield in National and International markets. PO10

CO5 Clarify the proposal for financial assistance and funding agencies PO5, PO8, PO9, PO10,
and reveal the modern methods employed in artificial bee hives. PO11

Text Books
 83

1. Dewey M. Caron. (2013). Honey Bee Biology and Beekeeping. Revised Edition. Wicwas
Press, Kalamazoo. ISBN 10: 1878075292
2. R. A. Morse. (1993). Rearing queen honey bees. Wicwas press, NY. ISBN-10 :
1878075055
3. Ted Hooper. (2010). Guide to Bees & Honey: The World's Best Selling Guide to
Beekeeping. Northern Bee Books. Oxford. ISBN 10: 1904846513
4. Jayashree K. V., Tharadevi C.S. and Arumugam N. (2014) Apiculture. Saras Publication
5. Raj H. (2020).Vinesh Text Book of Apiculture. S. Vinesh and Co.

References Books

1 Dewey M. Caron. (2020). The Complete Bee Handbook: History, Recipes, Beekeeping
Basics, and More,Rockridge Press. ISBN-10 : 1646119878
2 Joachim Petterson. (2016). Beekeeping: A Handbook on Honey, Hives & Helping the Bees,
Weldon Owen.
3 Eva Crane. (1999). The World History of Beekeeping and Honey Hunting. Routledge.
India.ISBN-10 : 0415924677
4 Pagar B. S. (2016). Textbook Of Apiculture. Sahitya Sagar.

5 Sehgal P.K. (2018). Text Book of Sericulture, Apiculture and Entomology.Kalayani.

Web Resources

Bee Keeping Basics. Retrieved from:https://denton.agrilife.org/files/2013/08/beekeeping-

1 basics.pdf

Beekeeping as an Entrepreneurship, Retrieved from:

2 https://lupinepublishers.com/agriculture-journal/pdf/CIACR.MS.ID.000270.pdf

Raising Bumble Bees at Home: A Guide to Getting Started. Retrieved from:

3 https://www.ars.usda.gov/ARSUserFiles/20800500/BumbleBeeRearingGuide.pdf

4 Apiculture – Biology for Everybody (homeomagnet.com)

5 Apiculture: Introduction to Apiculture (iasri.res.in)

Methods of Evaluation
Internal Continuous Internal Assessment Test 25 Marks
Evaluation Assignments
Seminars
 84

Attendance and Class Participation

External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S
CO2 S S S S
CO3 S S M S S
CO4 S M S S M
CO5 S S S S S

V- SEMESTER
Subject Subject Name Category L T P S Credit Inst. Marks
Code s
Hour CI Exter Tota
s A nal l

22MBUGC BACTERIOLO Core Y - - - 4 5 25 75 100

T5
GY AND Course
MYCOLOGY IX

Course Objectives
 85

CO1 Understand the role of normal flora and pathogenic microbes of various diseases and
clinical microbiological techniques.

CO2 Basic knowledge about Gram positive pathogenic bacteria and their epidemiology

CO3 Acquire knowledge about Gram negative pathogenic bacteria and nosocomial
infections

CO4 Comprehensive knowledge about medically important, its classification and its
significance

CO5 Gain knowledge about the general characteristics and mode of action of various
antibacterial agents

Unit Details No.of Course

Hours Objecti
ves

I History, Classification of Medically Important Microbes, Koch’s, 12 CO1

and River’s postulates-A brief account on the normal microbial
flora of the healthy human body – Host-pathogen interactions:
Definitions of infection, invasion, primary and opportunistic
pathogens, pathogenicity, virulence, toxigenicity, carriers,
endemic, epidemic, pandemic diseases and epidemiology –
putative virulence factors of human pathogens –infectious disease
cycle. Collection and transport of clinical specimens for bacterial
and fungal infections.

II Medically important Gram Positive infections - Causative agent, 12 CO2

clinical symptoms, pathogenesis, mode of transmission,
prevention and treatment of the following bacterial diseases (a)
Streptococcal infections (Streptococcus pyogenes, Streptococcus
faecalis), (b) Staphylococcal infections (Staphylococcus aureus),
(c) Tetanus (Clostridium tetani)(d) Diphtheria
(Corynebacteriumdiphtheriae) (e) Anthrax (Bacillus anthracis) (f)
Tuberculosis (Mycobacterium tuberculosis), (g) Leprosy
 86

(Mycobacterium leprae).

III Medically important Gram-Negative infections - Causative agent, 12 CO3

clinical symptoms, pathogenesis, mode of transmission,
prevention, and treatment of the following bacterial diseases (a)
Meningitis (Streptococcus pneumoniae, Neisseria meningitidis)
(b) typhoid (Salmonella typhi, Salmonella paratyphi) (c) cholera
(Vibrio cholerae) (d) bacillary dysentery (Shigelladysenteriae);
Sexually Transmitted disease (syphilis–
Treponemapallidum.Gonorrhoea - Neisseria gonorrhoeae);
Nosocomial infections – definition, importance, and their control
(Pseudomonas aeruginosa).

IV Medically important Fungi - Classification of medically important 12 CO4

fungi; Superficial mycoses: PityriasisVersicolor; TineaNigra;
Piedra. Cutaneous mycoses:
Microsporumspps.,Trichophytonspps., and
Epidermophytonfloccosum. Subcutaneous mycoses:
Chromoblastomycosis; Sporotrichosis; Systemic Mycoses
- Blastomycosis; Histoplasmosis; Opportunistic Infections -
Candidiasis; Cryptococcosis; Zygomycosis; Mycotoxins:
Aflatoxin

V Antimicrobial agents -General characteristics and mode of action 12 CO5

of Antibacterial agents: Modes of action with an example for each:
Inhibitor of nucleic acid synthesis; Inhibitor of cell wall synthesis;
Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism Antifungal agents: Mechanism of action
of Amphotericin B, Griseofulvin.

Total 60

Course Outcomes
 87

Course On completion of this course, students will;

Outcomes

CO1 Understand the importance of normal flora of human body and PO1, PO3, PO5,
acquire knowledge on the process of infectious disease. PO7, PO10, PO11

CO2 Explain the various bacterial pathological events during the PO1, PO3, PO5,
progression of an infectious disease, and apply the underlying PO7, PO10, PO11
mechanisms of spread of disease and its control.

CO3 Compile a list of disease-causing bacteria and compare their PO1, PO3, PO5,
modes of infection, symptoms, diagnosis and treatment. PO7, PO10, PO11

CO4 Comprehend human-fungal interaction, which can be applied to PO1, PO3, PO5,
obtain in-depth knowledge on fungal diseases and the PO7, PO10, PO11
mechanism behind the disease process.

CO5 Explain the types of mycoses caused in humans and categorize PO1, PO3, PO4,
the modes of infection, pathogenesis, and treatment with PO5,PO6,
PO7,PO9, PO10
introduction to mycotoxins.

Text Books

Tom Parker, M. Leslie H. Collier. (1990). Topley&Wilson’s Principles of

1
Bacteriology, Virology and Immunity,8th Edition. London: Edward Arnold.
Greenwood, D., Slack, R.B. and Peutherer, J.F. (2012) Medical Microbiology,
2
18thEdition. Churchill Livingstone, London.
Finegold, S.M. (2000) Diagnostic Microbiology, 10th Edition. C.V. Mosby
3
Company, St. Louis.
Ananthanarayanan, R. and JayaramPanicker C.K. (2020) Text book of Microbiology.
4
Orient Longman, Hyderabad.
JagdishChander (2018). Textbook of Medical Mycology, 4th edition, Jaypeebrothers
5
medical publishers.
References Books

1 Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (Editions) (1994) Methods
for General and Molecular Bacteriology. ASM Press, Washington, DC.
 88

2 Kevin Kavanagh, (2018). Fungi Biology and Applications 3rd Edition. Wiley
Blackwell publishers.
3 C.J. Alexopoulos, C.W. Mims, M. Blackwell, (2007). Introductory Mycology, 4th
edition. Wiley publishers.
4 A.J. Salle (2007). Fundamental principles of bacteriology, fourth edition, Tata
McGraw-Hill Publications.
5 Christopher C. Kibbler ,Richard Barton,Neil A. R. Gow, Susan Howell,Donna M.
MacCallum, Rohini J. Manuel (2017). Oxford Textbook of Medical Mycology.
Oxford University Press.
Web Resources

1 http://textbookofbacteriology.net/nd
2 https://microbiologysociety.org/members-outreach-resources/links.html
3 http://mycology.cornell.edu/fteach.html
4 https://www.adelaide.edu.au/mycology/
5 https://www.isham.org/mycology-resources/mycological-links
Methods of Evaluation
Continuous Internal Assessment Tests 25 Marks

Internal
Evaluation
Assignments
Seminars
Attendance and Class Participitation
External End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions
Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Analyse Problem-solving questions, Finish a procedure in many steps, Differentiate
(K4) between various ideas, Map knowledge
Evaluate Longer essay/ Evaluation essay, Critique or justify with pros and cons
 89

(K5)
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations

Mapping with Programme Outcomes

CO/PO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S S M S
CO2 S S S S M S
CO3 S S S S M S
CO4 S S S S M S
CO5 S S M S M S S M

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits
Hour CI Exter Total
s A nal

22MBU VIROLOGY AND CORE Y - - - 4 5 25 75 100

GCT6 COURSE
PARASITOLOGY
X

Course Objectives

CO1 To gain knowledge on properties and classification of viruses and collection of relevant
clinical samples for diagnosing viral infections.

CO2 To understand pathogenic microorganisms of viruses and the mechanisms by which they
cause disease in the human body.

CO3 To gain knowledge about reemerging viral infections and develop diagnostic skills, including
the use and interpretation of laboratory test in the diagnosis of infectious diseases.

CO4 Understand the types of parasites causing infections in the intestine.

CO5 To develop skills in the diagnosis of parasitic infections.

 90

Unit Details No.of Course

Hours Objectives

I General Properties, replication and Classification of viruses 12

(Baltimore classification), Cultivation of viruses- in animals, CO1
embryonated eggs and tissue culture, Virus purification assays -
collection and transport of clinical specimens for viral infections.

II Viral diseases with reference to symptoms, pathogenesis, 12 CO2

transmission, prophylaxis and control – Arboviruses (Flavi virus),
Picorna viruses (Polio virus and Rhinovirus), Hepatitis viruses
(HAV, HBV, HCV, HDV, HEV), Rabies virus, Orthomyoviruses
(Influenza virus) and Paramyxoviruses (Mumps and Measles virus),
Pox viruses (Variola, Vaccinia), Herpes viruses (Herpes simplex,
Varicella zoster), Adeno viruses, Rota viruses and HIV viruses.
Oncogenic viruses (Human Papilloma virus): Introduction,
characteristics of transformed cells, mechanism of viral oncogenesis
and clinical manifestations.
III Emerging and reemerging viral infections (SARS, Swine flu, Ebola, 12 CO3
Dengue, Chikungunya- and Corona) – causes, spread and preventive
measures. Detection of viruses in clinical specimens – Serological
and Molecular diagnosis of virus infections – Antiviral agents,
Interferons and Viral Vaccines, Immunization schedules.
IV General introduction to Medical Parasitology, Classification of 12 CO4
medically important parasites. Morphology, life cycle, pathogenesis,
clinical features, laboratory diagnosis, prevention and treatment of
diseases caused by the following organisms: Entameobahistolytica,
flagellates (Giardia lamblia, Leishmaniadonovani), Sporozoa-
Plasmodiumspps.
V Introduction to Helminthes, Platyhelminthes – Taenia – Fasciola – 12 CO5
Paragonimus – Schistosomaspps.. Nemathelminthes – Ascaris–
Ankylostoma – Enterobius – Trichuris – Trichinella – Wuchereria –
Dracanculus. Collection, transport and examination of specimen
 91

Laboratory techniques in parasitology Examination of faeces for ova

and cyst by direct wet mount and iodine wet mount, Concentration
methods (Floatation and Sedimentation techniques), Examination of
blood for parasites. Cultivation of parasites.

Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes

CO1 Understand the structure and properties of viruses, cultivation PO5,PO10

methods and diagnosis of viral diseases.

CO2 Knowledge of basic and general concepts of causation of disease PO5,PO10

by the pathogenic microorganisms and various parameters of
assessment of their severity and the methods of diagnosis.
CO3 Insights to treatment options of viral diseases. PO5,PO10

CO4 Knowledge about the importance of protozoans in the intestine. PO5,PO10

CO5 Knowledge of Nematodes as infectious agent PO5,PO10

TEXT BOOKS

1. S., Rajan(2007). Medical microbiology, MJP publisher.

2. JeyaramPaniker, C.K. (2006). Text Book of Parasitology Jay Pee Brothers,NewDelhi.

3 AroraD.R. and AroraB. (2002). Medical Parasitology, 1stEdition CBS Publishers &
Distributors, New Delhi.

4 Chatterjee (1986). Medical Parasitology. Tata McGraw Hill, Calcutta.

5 Parija S. C. (1996). Text Book of Medical Parasitology.4th edition, Orient Longman,

AllIndia Publishers & Distributors.

References Books

1 Jawetz, E., Melnick, J.L. and Adelberg, E.A. (2000). Review of Medical Microbiology,
 92

19thEdition. Lange Medical Publications, U.S.A.

2 Ananthanarayan, R. and JeyaramPaniker, C.K. (2009). Text Book of Microbiology,

8thEdition. Orient Longman, Chennai .

3 Conrat HF, Kimball PC and Levy JA. (1988). Virology. II edition. Prentice Hall,

Englewood Cliff, New Jersey..

4 Topley& Wilsons’s (1990). Principles of Bacteriology, Virology and Immunity, 8 th

Edition, Vol. III Bacterial Diseases, Edward Arnold, London.

5 Finegold, S.M. (2000). Diagnostic Microbiology, 10th Edition. C.V. Mosby

Company,St.Louis.

Web Resources

1 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047123/

2 https://www.ncbi.nlm.nih.gov/pubmed/21722309

3 https://www.sciencedirect.com/science/article/pii/S2211753919300193

4 https://cmr.asm.org/content/30/3/811

5 https://www.nejm.org/doi/full/10.1056/NEJMoa1811400

Methods of Evaluation

Continuous Internal Assessment Test

Assignments
Internal Seminars 25 Marks
Evaluation
Attendance and Class Participation
External
Evaluation End Semester Examination 75 Marks

Total 100 Marks

Methods of Assessment
 93

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /

Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or

overview
(K2)

Application
Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate
between various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 M M

CO2 M M

CO3 M M

CO4 M M

CO5 M M

Subject Subject Name Categor L T P S Credit Inst. Marks

Code y s
Hour CIA Externa Total
s l

22MBUGCP PRACTICAL V Core Y - - - 4 5 40 60 100

5 course
XI

Course Objectives

CO1 Learning Objectives

 94

To familiarize students with medical microbiology techniques and technical knowledge

on collection and processing of clinical samples.

CO2 To learn the techniques for isolation and identification of bacterial pathogens.

CO3 To gain expertise in various techniques of clinically important viral pathogens and their
identification.

CO4 To get acquainted with medically important fungi and their metabolism.

CO5 To categorize parasites and understand their role in infections.

Unit Details No.of Course

Hours Objectives

I 1. Collection and Transport of Clinical specimens. 12

2. Simple, Differential and Special staining of Clinical CO1
materials.
3. Culture techniques used to isolate microorganisms.
II 4. Identification of bacterial pathogens by their biochemical 12 CO2
reactions.
5. Antimicrobial susceptibility testing by disc-diffusion
technique and determination of Minimum Inhibitory
Concentration.
III 6. Isolation of Bacteriophages from Sewage and other natural 12 CO3
sources.
7. Identification of Viruses in Slides/Smears/Spotters.
Demonstration of Negri bodies (Staining).
8. Cultivation of Viruses in Embryonated eggs – Amniotic,
Allantoic, Yolk sac routes and Chorio-allantoic membrane.
IV 9. Microscopic identification of medically important Fungi – 12 CO4
KOH and Lactophenol cotton Blue staining.
10. Slide culture techniques for fungal Identification
11. Identification of Dermatophytes.
12. Germ tube test, Carbohydrate fermentation and assimilation
 95

tests for Yeasts.

V 13. Direct Examination of Faeces – wet mount and Iodine mount 12 CO5
– Demonstration of Protozoan cysts and Helminthes eggs.
14. Concentration techniques of stool specimen – Floatation and
Sedimentation methods.
15. Examination of blood for Malarial parasites – thin and thick
smear preparations.
16. Identification of Medically important parasites in slides /
specimens as spotters.
Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Demonstrate methods to observe and measure microorganisms by PO4, PO5, PO7.
standard microbiological techniques
CO2 Identify pathogenic microorganisms in the laboratory set-up and PO4, PO5, PO7, PO8.
interpret their sensitivity towards commonly administered
antibiotics.
CO3 Understand experimental tools used to cultivate and characterize PO4, PO5, PO7, PO8.
clinically important viruses and bacteriophages
CO4 Elucidate clinically important fungi. PO4, PO5, PO7, PO8.

CO5 Investigate Parasites of medical importance and identify them PO4, PO5, PO7, PO8.
from clinical specimens.
Text Books

1. Dubey, R.C. and Maheswari, D.K. (2020). S. Chand Publishers. ISBN-13: 978-
8121921534, ISBN-10: 8121921538.

2. K.R. Aneja (2017). Experiments in Microbiology, Plant Pathology, Tissue Culture and
Microbial Biotechnology. 5th Edition. New Age International Publishers. ISBN-10:
9386418304, ISBN-13: 978-9386418302.
 96

3 Collee, J.G., Fraser, A.G., Marnion, B.P. and Simmons, A. (1996). Mackie & McCartney
Practical Medical Microbiology. 14th Edition. Elsevier. ISBN-10: 813120393X, ISBN-13:
978-8131203934.

4 Prince CP (2009). Practical Manual of Medical Microbiology, Ist edition, Jaypee digital
publishing.

5 James H. Jorgensen, Karen C. Carroll, Guido Funke, Michael A. Pfaller, Marie Louise
Landry, Sandra S. Richter, David W. Warnock (2015). Manual of Clinical Microbiology,
11th Edition, ASM press

References Books

1 Patricia M. Tille (2021). Bailey & Scott’s Diagnostic Microbiology, 15th Edition. Elsevier.
ISBN-10: 0323681050, ISBN-13: 978-0323681056.

2 Monica Cheesbrough (2006). District Laboratory Practice in Tropical Countries. Part 1.

2nd Edition. Cambridge University Press. ISBN-10: 0521171571, ISBN-13: 978-
0521171571.

3 Michael A. Pfaller (ed.) (2015). Manual of Clinical Microbiology. Vol. 1 and 2. 11th
Edition. ASM Press. ISBN-10: 9781555817374, ISBN-13: 978-1555817374.

4 Josephine A. Morello, Paul A. Granato and Helen EckelMizer (2002). Laboratory Manual
and Workbook in Microbiology. 7th Edition. The McGraw Hill Company. ISBN: 0-07-
246354-6.

5 Rowland, S.S., Walsh, S.R., Teel, L.D. and Carnahan, A.M. ((1994). Pathogenic and
Clinical Microbiology: A Laboratory Manual. Lippincott Williams & Wilkins. ISBN-10:
0316760498, ISBN-13: 9780316760492.

Web Resources

1 https://www.microcarelab.in/media/microcarelab.in/files/Sample-Collection-Manual.pdf

2 http://ssu.ac.ir/cms/fileadmin/user_upload/Daneshkadaha/pezeshki/microb/
file_amuzeshi/Lab_QA_Microbiology_QA.pdf

3 https://www.academia.edu/11977315/
Basic_Laboratory_Procedures_in_Clinical_Bacteriology

4 https://cmr.asm.org/content/31/3/e00062-17.full.pdf
 97

5 https://microbiologyinfo.com/techniques-of-virus-cultivation/

Methods of Evaluation

Continuous Internal Assessment Test 25 Marks

Internal
Evaluation Assignments

Seminars

Attendance and Class Participation

External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment

Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions

Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S M S
CO2 S S S L
CO3 S S S L
CO4 S S S L
CO5 S S S L

Subject Subject Name Category L T P S Credit Inst. Marks

 98

Code s Hour CI Exter Total

s A nal
22MBU GROUP Project - - - - 4 5 40 60 100
GCPR PROJECT with
Viva-
Voce
CC-XII

Group projects enable students to get hands-on training in microbiological techniques needed for

research. Thus the students can share diverse perspectives resulting in pooling of knowledge and

skills. Group work may approach tasks and solve problems in novel, interesting ways, thereby

converting established theoretical concepts to practical skills. If structured properly, it will

promote team work and collaboration. Group projects also will help students to choose a

research design, solve real life problems and benefit the society at large. Thus group project

facilitates the students to convert ideas to practice thereby creating a research culture among

students.

Guidelines for group project:

A research problem need to be selected based on creative ability and scientific thought.

A brief description of the problem needs to be given.

Hypothesis statement should be framed.

Objectives by which the project work is to be carried out should be clearly stated.

Methodology has to be designed to test the hypothesis.

Results obtained need to be replicable.

Documented report has to be submitted on completion of the project.

Subject Subject Name Category L T P S Credit Inst. Marks

Code s Hour CI Exter Total
 99

s A nal
22MBU RECOMBINANT ELECTI Y - - 3 4 25 75 100
GDE5 DNA VE
TECHNOLOGY GENERI
C/
DISCIP
LINE
SPECIFI
C
ELECTI
VE- V
Course Objectives

CO1 Understand the principles of rDNA technology.

CO2 Illustrate the molecular tools employed in gene cloning.

CO3 Discuss the importance of various molecular techniques and their importance in
Biotechnology.

CO4 Acquire knowledge about the concepts of tissue culture methods and transgenic
organisms.

CO5 Examine recent trends in genetic engineering and its application in human welfare.

Unit Details No. of Course

Hours Objectives
I MilestonesinrDNATechnology-GeneManipulation- 12
CO1
StepsinvolvedinGeneCloning.Isolation of Chromosomal and
Plasmid DNA. Restriction endonuclease - Discovery,
Types,Mode of action-Application of
Ligase,DNAPolymerase,DNA Modifying
enzymesandTopoisomerases.UseofLinkersandAdapters.
II ArtificialGeneTransfermethods- 12 CO2
CalciumChlorideInduction,Electroporation,Microinjection,
Biolistic method, Liposome and Viral-mediated
delivery.Cloning vectors –Properties and Applications -
Plasmid Based Vectors- Natural Vectors-pSC101 and
pMB1.Artificial Vectors- pBR322 and pUC.Phage Based
 100

Vectors- Lambda phage. Hybrid Vectors,Phagemid, Cosmid,

BAC and YAC.Screening of Recombinants.Genomic DNA
and cDNAlibrary-ConstructionandScreening.
III Molecular Tools- PCR- Types. Gel Electrophoresis- AGE 12 CO3
and PAGEBlottingTechniques-
Southern,Western&Northern.DNAsequencingmethods-
Sanger’sandAutomated method. Recent Trends in Genetic
Engineering- Targeted Genome Editing-
ZFNs,TALENs,CRISPRs.GeneTargeting-Knock-in &Knock-
outs.DNAFingerPrinting,
IV Plant Biotechnology – Media, Growth Regulators and 12 CO4
Equipment for Plant Tissue Culture-Explant Culture-
Micropropagation- Callus and Protoplast Culture-
Production of Bio-ActiveSecondary Metabolites by Plant
Tissue Culture -Agrobacterium and Crown Gall Tumors,
TiPlasmidandRiPlasmid-AnimalBiotechnology-
PrinciplesofAnimalCellCulture,MediaandEquipment for
Animal Cell Culture – Primary and Secondary Cultures-
Cell Lines-
Types,EstablishmentandMaintenanceofCellLines.
V Applications of Genetic Engineering - Transgenic Animals 12 CO5
– Mice and Sheep-RecombinantCytokines and their use in
the Treatment of Animal infections- Monoclonal Antibodies
inTherapy- Vaccines and their Applications in Animal
Infections - Human Gene Therapy-
GermlineandSomaticCellTherapy-Ex-vivoGeneTherapy-
SCID(SevereCombinedImmunoDeficiency) – In-vivo Gene
Therapy- CFTR (Cystic Fibrosis Transmembrane
Regulator) –Vectors inGeneTherapy-ViralandNon-
ViralVectors.TransgenicPlants– BtCotton,BtCorn,
RoundReadysoybean,FlavrSavrTomatoandGoldenRice.
Total 60
 101

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Illustrate the steps involved in introduction and expression PO4, PO6, PO7, PO9
of foreign DNA into bacteria, animal and plants cells and
their screening.

CO2 Discuss the various cloning vectors and their applications. PO4, PO6, PO7, PO9

CO3 Assess the usage and advantages of molecular tools. PO4, PO6, PO7, PO9

CO4 Explain plant and animal tissue culture protocols and gene PO4, PO6, PO7, PO9
transfer mechanism.

CO5 Elucidate and understand the application of genetic PO4, PO6, PO7, PO9
engineering and gene therapy.
Text Books

1. Brown T.A.(2016). Gene Cloning and DNA Analysis. 7thEdition . John Wiley and
Jones, Ltd.

2. Dale J. W., Schantz M.V. and Plant N. (2012). From Gene to Genomes – Concepts
and Applications of DNA Technology. 3rd Edition. John Wileys and Sons Ltd.

3. Keya Chaudhuri (2013). Recombinant DNA technology. The Energy and Resources
Institute
4. Siddra Ijaz, Imran UlHaq (2019). Recombinant DNA Technology. Cambridge
Scholars Publishing.
5. Monika Jain (2012). Recombinant DNA Techniques: A Textbook, I Edition,Alpha
Science International Ltd
References Books

1. Maloy S. R., Cronan J.E. Jr. and FreifelderD.(2011). Microbial Genetics. 2nd Edition.
Narosa Publishing Home Pvt Ltd.
2. Glick B. R. and Patten C.L.(2018). Molecular Biotechnology – Principles and
Applications of Recombinant DNA. 5th Edition. ASM Press.
3. Russell P.J. (2010). iGenetics - A Molecular Approach, 3 rd Edition. Pearson New
International Edition.

4. Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics
of Bacteria,4th Edition. ASM Press Washington-D.C. ASM Press.

5. James D.Watson, Michael Gilman, Jan Witkowski, Mark Zoller (1992). Recombinant
DNA. Scientific American Books
 102

Web Resources

1 https://www.britannica.com/recombinant-DNA-technology
2 https://www.byjus.com/recombinant-dna-technology
3 https://www..rpi.edu
4 https://www..ncbi.nlm.nih.gov
5 https://www.le.ac.uk/recombinant-dna-and-genetic-techniques
Methods of Evaluation
Continuous Internal Assessment Test 25 Marks
Internal Assignments
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate
between various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S L S S M S
CO2 S L S S M S
CO3 S L S S M S
CO4 S L S S M S
CO5 S L S S M S
 103

Subject Subject Category L T P S Cr Inst. Marks

Code Name edi
ts Hour CI Exter Total
s A nal

22MBUGD BIOSAFETY CORE Y - - - 3 4 25 75 100

E6
&BIOETHIC ELECTIV
E VI
S

Course Objectives

CO1 To create a research environment - encourage investigation, analysis and studying the
bioethical principles, values, concepts, and social and juridical implications contained in the
Universal Declaration on Bioethics and Human
CO2 Rights in order to assist their application and promotion in the areas of science,
biotechnology and medicine.
CO3 To discuss about various aspects of biosafety regulations, IPR and bioethics concerns arising
from the commercialization of biotech products.
CO4 To introduce fundamental aspects of Intellectual property Rights to students who are going to
play a major role in development and management of innovative projects in industries.
CO5 To understand the importance of IPR, Patents and Patent laws.

Unit Details No.of Course

Hours Objectives

I Basics of Biosafety - Laboratory Hazards and Hazard symbols. 12 CO1

Definitions on Biohazard, Biosafety and Biosecurity- Biohazard-
LAI, BP. Biohazard Classification. Biological Risk Groups. Need
and application of biosafety. Good Laboratory Practices (GLP),
Good Manufacturing Practices (GMP).

II Hazardous materials in Biotechnology - Categories of Waste in the 12 CO2

Biotechnology Laboratories, Biohazardous waste and their disposal
and treatments- issues in use of GMO’s, risk for animal/human/
agriculture and environment owing to GMO. Hazardous materials,
 104

Emergency response/ first aids in Laboratories.

III Biological Safety Containment in Laboratory - Primary and 12 CO3

secondary containments - Physical and biological containment.
Types of biosafety containments (level I, II, III), PPE, Biosafety
guidelines in India - Roles of Institutional Biosafety Committee,
RCGM, GEAC.

IV Introduction and need of Bioethics - its relationship with other 12 CO4

branches, Ethical implications of biotechnological products and
techniques. Ethical Issues involving human cloning, human genome
project, prenatal diagnosis, agriculture and animal rights, Social and
ethical implications of biological weapons.

V IPR, Patents and Patent laws - Intellectual property rights-TRIP- 12 CO5

GATT International conventions patents, Methods of application of
patents, Legal implications. Biodiversity and farmer rights,
Objectives of the patent system, Basic principles and general
requirements of patent law, Biotechnological inventions, and patent
law. Legal development-Patentable subjects and protection in
biotechnology. The patenting of living organisms.

Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes

CO1 Understand the control measures of laboratory hazards (chemical, PO1, PO2, PO3, PO7,
biological and physical) and to practice safety strategies and PO10
personal protective equipment
CO2 Develop stratagems for the use of genetically modified organisms PO1, PO3, PO4
and Hazardous materials
CO3 Develop skills of critical ethical analysis of contemporary moral PO1, PO6
problems in medicine and health care.
CO4 Analyze and respond to the comments of other students regarding PO3, PO4
 105

philosophical issues.

CO5 Pave the way for the students to catch up Intellectual Property(IP) as PO1, PO7, PO10
a career option a. R&D IP Counsel b. Government Jobs – Patent
Examiner c. Private Jobs d. Patent agent and Trademark agent e.
Entrepreneur
Text Books

1. Usharani .B, S Anbazhagi, C K Vidya, (2019). Biosafety in Microbiological Laboratories- 1 st

Edition, Notion Press, ISBN-101645878856

2. Satheesh.M.K.,(2009). Bioethics and Biosafety- 1st Edition, J. K International Publishing

House Pvt. Ltd: Delhi, ISBN :9788190675703

3 DeepaGoel and ShominiParashar, (2013). IPR, Biosaftey and Bioethics- 1 st Edition, Pearson
education: Chennai, ISBN-13: 978-8131774700

4 Rajmohan Joshi (2006). Biosafety and Bioethics. Gyan Books publisher.

5 Sateesh. M.K. (2013). Bioethics and Biosafety. i.K. International pvt,Ltd.

References Books

1 Nithyananda, K V. (2019). Intellectual Property Rights: Protection and Management, India,

IN: Cengage Learning India Private Limited, ISBN-10: 9386668572
2 Neeraj, P., &Khusdeep, D. (2014). Intellectual Property Rights, India, IN: PHI learning
Private Limited, ISBN : 9788120349896
3 Ahuja, V K. (2017). Law relating to Intellectual Property Rights, India, IN: Lexis Nexis,
ISBN-10: 8131251659.
4 Edited by Sylvia Uzochukwu, Nwadiuto (Diuto) Esiobu, Arinze Stanley Okoli, Emeka
Godfrey Nwoba, EzebuiroNwagboChristpeace, Charles OluwaseunAdetunji, Abdulrazak B.
Ibrahim, Benjamin Ewa Ubi (2022). Biosafety and Bioethics in Biotechnology-Policy,
Advocacy, and Capacity Building,1st edition. CRC Press
5 Sree Krishna. V (2007). Bioethics and Biosafety in Biotechnology. New age international
publishers.

Web Resources

1 Subramanian, N., &Sundararaman, M. (2018). Intellectual Property Rights – An Overview.

 106

Retrieved from http://www.bdu.ac.in/cells/ipr/docs/ipr-eng-ebook.pdf.

2 World Intellectual Property Organisation. (2004). WIPO Intellectual propertyHandbook.

Retrieved from https://www.wipo.int/edocs/pubdocs/en/intproperty/489/wipo_pub _489.pdf.

3 https://www..niehs.nih.gov/bioethics

4 https://www.sist.sathyabama.ac.in

5 https://www.longdom.org/bioethics-and-biosafety

Methods of Evaluation

Continuous Internal Assessment Test 25 Marks

Internal Assignments
Evaluation
Seminars

Attendance and Class Participation

External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks

Methods of Assessment

Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions

Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO PO7 PO8 PO9 PO10 PO11
 107

6
CO1 S S S M M
CO2 S S S
CO3 S S
CO4 S S
CO5 S M S

VI - SEMESTER

Subject Subject Name Cate L T P S Credit Inst. Marks

Code gory s Hour CI Exter Total
s A nal
ENVIRONMENTAL COR Y - - - 4 6 25 75 100
22MBU E
AND
GCT7 COU
AGRICULTURE RSE
–XIII
MICROBIOLOGY

Course Objectives

CO1 To discuss the distribution and association of microorganism in various ecosystems and to
know about the role of microorganism in water pollution and water quality.

CO2 To acquire knowledge about the role of microorganism in water pollution and water quality

CO3 Gain knowledge about microbes as biofertilizers and the aspects of application.

CO4 To learn about the process of solid waste management and sewage water treatment.

CO5 Gain knowledge on various plant diseases and pathogens

Unit Details No. of Course

Hours Objective
s
I Microorganisms and their Habitats: Structure and function of 12 CO1
ecosystems
Terrestrial Environment: Soil profile and soil microflora, Microbial
succession in decomposition of soil organic matter. Role of
 108

microorganisms in elemental cycles in nature: Carbon, Nitrogen.

Aquatic Environment: Microflora of fresh water and marine habitats,
factors influencing microbial growth in the aquatic environments.
Atmosphere: Aeromicroflora and dispersal of microbes, Assessment of
air quality, Enumeration of microorganism in air, Air sanitation.
Extreme Habitats: Extremophiles: Microbes thriving at high & low
temperatures, pH, high hydrostatic & osmotic pressures, salinity, &
low nutrient levels.
Predisposing factors for Environmental diseases – infectious (water and
air borne) and pollution related, spread and control of these diseases.
Environmental Protection Agency (EPA) - role in environmental
protection.
II Water potability: Sources and types of water surface, ground, stored, 11 CO2
distilled, mineral and de-mineralized water and their pollution,
biological indicators of water Pollution, Eutrophication. Conventional
Bacteriological standards of Water Quality, MPN index, coliform test,
Membrane filtration. BOD, COD. Advanced molecular methods for
water analysis. Water borne diseases. Central Pollution Control Board
(CPCB) standards.
III Microbial Interactions: Rhizosphere microflora. Concepts of Nitrogen 12 CO3
fixation – Symbiotic and asymbiotic nitrogen fixers.Brief account of
microbial interactions: Symbiosis, neutralism, commensalism,
competition, Ammensalism, Synergism, parasitism, and predation.
General account and Significance of Biofertilizers and biocontrol
agents – Bacterial, cyanobacterial, VAM. Mass production of
Rhizobialbiofertilizer. Biocontrol agents – Bacterial, viral, fungal.
IV Waste treatment and bioremediation: Solid waste management: 15 CO4
Sources and types of solid waste, composting, vermin composting,
production of biogas. Liquid waste management: Primary, secondary,
and tertiary sewage treatment. Bioremediation and waste management:
Need and scope of bioremediation. Degradation of hydrocarbons, oil
 109

spills, heavy metals – Chromium, lead, and xenobiotics – PCB.

V Plant pathology: Mode of entry of pathogens, Microbial enzymes, 10 CO5
toxins, growth regulators and suppressor of plant defense in plant
diseases. Plant defense mechanisms. Bacterial diseases – Citrus canker,
Blight of paddy. Viral disease – TMV, CMV. Fungal disease- red rot of
sugarcane, Tikka disease. Plant disease management.
Total 60
Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Describe about the structure and function of ecosystems and PO1
understand the role of microbes in various environments
CO2 Identify the cause of water pollution, and perform methods to assess PO4,PO5,PO6,PO7,
PO8
the quality of water.
CO3 Explain the productionof biofertilizers and biopesticides. PO1, PO7,PO8
CO4 Explainabout waste treatment process and microbial decomposition PO6
and bio-remediation process.
CO5 Describe about plant diseases caused by microbes and acquire a clear PO1,PO5
idea on plant pathogenic interaction
Text Books

1. Joseph C. Daniel. (2006). Environmental aspects of Microbiology 2nd Edition. BrightSun

Publications.

2. Pradipta. K.M. (2008). Textbook of Environmental Microbiology.I.K.Publishing. House.

3. Ramanathan, and Muthukaruppan SM. (2005). Environmental

Microbiology.OmSakthiPathipagam, Annamalai Nagar.

4. K. Vijaya Ramesh.(2004).Environmental Microbiology. 1st Edition. MJP Publishers.

5. SubbaRao.N.S.(2017). Soil Microbiology.4th Edition. Oxford and IBH Publishing Pvt.Ltd.

References Books

1 Dirk, J. Elasas, V., Trevors, J.T., Wellington, E.M.H. (1997). Modern Soil
 110

Microbiology, Marcel Dekker INC, New York, Hong Kong.

2 EcEldowney S, Hardman D.J., Waite D.J., Waite S.(1993). Pollution: Ecology and
Biotreatment – Longman Scientific Technical.

3 Mitchel, R.(1992). Environmental Microbiology. Wiley –John Wiley and Sons. Inc.
Publications, New York.
4 Clescri, L.S., Greenberg, A.E. and Eaton, A.D.(1998). Standard Methods for
Examination of Water and Wastewater, 20thEdition. American Public Health Association.
5 Atlas, R.M. and Bartha, R.(1992). Microbial Ecology: Fundamentals andApplications, 2nd
Edition. The Benjamin / Cummings Publishing Co.,Redwood City, CA.

Web Resources
1 https://nptel.ac.in/courses/126105016

2 https://www.classcentral.com/course/swayam-plant-pathology-and-soil-health-14236

3 https://www.wasteonline.org.uk/resources/InformationSheets/WasteDisposal.htm

4 https://plantpath.cornell.edu/labs/enelson/PDFs/Hill_et_al_2000.pdf

5 https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2389.2005.00781.x

Methods of Evaluation
Continuous Internal Assessment Test 25 Marks
Internal Assignments
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions
Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between
various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations
 111

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S
CO2 M S S S S
CO3 S S S
CO4 S
CO5 M M

Subject Subject Name Cate L T P S Cr Inst. Marks

Code gory edi Hour CI Exter Total
ts s A nal
22MBU FOOD, DAIRY COR Y - - - 4 6 25 75 100
GCT8 E
AND PROBIOTIC
COU
MICROBIOLOGY RSE
- XIV
Course Objectives

CO1 To impart current knowledge of basic and applied microbiological aspects of fluid milks
and dairy products for improved quality and food safety.
CO2 Gives an insight into various types of food borne diseases and their prevention
CO3 To gain information about microflora of milk

CO4 To study about the production of fermented dairy products

CO5 To impart current knowledge of probiotics, prebiotics and functional dairy foods for the
health benefits
To create a sustainable environmentally and technologically advanced dairy farm
UNIT Details No.of Course
Hours Objective
s
I Food as a substrate for micro organisms-.Micro organisms important 12
CO1
in food microbiology; Molds, yeasts and bacteria -General
Characteristics - Classification and importance. Principles of food
preservation - Asepsis - Removal of micro organisms, - High
temperature - Low temperature - Drying - Food additives.
 112

Nanoscience in food preservation; microencapsulation.

II Contamination and spoilage of food products -Food borne infections 15 CO2
(Bacillus cereus, ,Salmonellosis, Shigellosis, ,Listeria monocytogenes
and Campylobacter jejuni) and intoxications – (Staphylococcus
aureus, Clostridium botulinum ,Clostridium perfringens and
mycotoxins) Food borne disease outbreaks - newly emerging
pathogens. Conventional and Novel technology in control of food
borne pathogens and preventive measures - Food sanitation - plant
sanitation - Employees’ health standards. Regulatory Agencies
&criteria for food safety.
III Microflora of raw milk - sources of contamination. Spoilage and 15 CO3
preservation of milk and milk products. -antimicrobial systems in raw
milk. Importance of biofilms, their role in transmission of pathogens
in dairy products and preventive strategies.
IV Food fermentations: Indian Pickles Bread,vinegar, fermented 15 CO4
vegetables (sauerkraut), fermented dairy products (yoghurt, cheese,
AcidophilusMilk,Kefir,Koumiss). Oriental fermented foods-Miso –
Tempeh Ontjom . Natto, Idli Spoilage and defects of fermented dairy
products -. Functional fermented foods and nutraceuticals, bioactive
proteins and bioactive peptides, genetically modified foods.
V Probiotic microorganisms, concept, definition safety of probiotic 15 CO5
microorganisms, legal status of probiotics Characteristics of
Probiotics for selection: stability maintenance of probiotic
microorganisms. Role of probiotics in health and disease: Mechanism
of probiotics. Application of bacteriocins in foods.Biopreservation.
Prebiotics: concept, definition, criteria, types and sources of
prebiotics, prebiotics and gut microflora - Prebiotics and health
benefits: mineral absorption, immune response, cancer prevention,
elderly health and infant health, prebiotics in foods.

Total 72
 113

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Gain knowledge about food as a substrate for various microbes, PO7,PO8,PO10
Understand about the principles and application of different types
of food spoilage and preservation technique,
CO2 Acquire a thorough understanding of food borne diseases, testing PO5,PO10
methods, and preventive technique
CO3 Gain information about spoilage of milk and its products and its PO5,PO7
antimicrobial properties
CO4 Learn about the various fermented product and its various stage PO7,PO8,PO10
spoilage
CO5 Impart current knowledge of probiotics, prebiotics and functional PO5,PO6
dairy foods for the health benefits
Text Books

1. Frazier WC and West off DC. (2017). Food microbiology. 5 th Edition TATA McGraw
Hill Publishing Company Ltd. New Delhi.

2. Adams, M.R., Moss, M.O.(2018). Food Microbiology 1 stedition. New Age Publishers by
New Age International (P) Ltd., Publishers.

3 R.C. Dubey. (2014). Advanced Biotechnology. S. Chand publishers.

4 Banwart GJ. (1989). Basic food microbiology, Chapman & Hall, New York.
5 Sugumar D. (1997). Outlines of dairy technology, Oxford University press. 1997.
References Books
1 Jay JM, Loessner MJ and Golden DA.(2005). Modern Food Microbiology. 7th Edition
CBS Publishers and Distributors, Delhi, India.
2 Prescott, Harley and Klein Wim.(2008). Microbiology, 7 th Edition McGraw Hill
Publications.
3 Robinson, R. K.(2002). Dairy Microbiology Handbook - The Microbiology of Milk and
Milk Products (Third Edition), A John Wiley & Sons, Inc., New York.
 114

4 Yuankunlee,Sepposalminen. (2008). Handbook of probiotics and prebiotics Second

Edition. A John Wiley & Sons publication Inc.
5 DharumauraiDhansekaran, AlwarappanSankaranarayanan. (2021). Advances in Probiotics
Microorganisms in Food and Health 1st Edition. eBook ISBN:9780128230916.
WEB RESOURCES

1 https://www.researchgate.net/publication/
15326559_A_Dynamic_Approach_to_Predicting_BacterialGrowth_in_Food/link/
5a1d2e02aca2726120 b28eba/download
2 https://www.fda.gov/food/laboratory-methods-food/bam-foodsamplingpreparation-
sample-homogenate
3 https://www.researchgate.net/publication/243462186_Foodborne_diseases_in_India_-
_A_review
4 https://www.researchgate.net/publication/
228662659_Fermented_Dairy_Products_Starter_Cultures_and_Potential_Nutritional_Ben
efits/link/000084160cf23f86393d5764/
download
5 https://www.fda.gov/food

Methods of Evaluation

Continuous Internal Assessment Test 25 Marks

Assignments
Internal Seminars
Evaluation
Attendance and Class Participation
External
Evaluation End Semester Examination 75 Marks

Total 100 Marks

Methods of Assessment

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

 115

Understand /

Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)

Application
Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse
Problem-solving questions, Finish a procedure in many steps, Differentiate between
(K4) various ideas, Map knowledge
Evaluate
(K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons

Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or

Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S M
CO2 S M
CO3 S M
CO4 S S M
CO5 M M

Subject Subject Name Category L T P S Credit Inst. Marks

Code s Hour CI Exter Total
s A nal
22MBU PRACTICAL CORE Y - - - 4 6 25 75 100
GCP6 COURSE
VI
– XV-
PRACTI
CAL VI

Course Objectives

CO1 Toassess the water quality and potability.

 116

CO22M To acquire knowledge on enumeration of bacteria from milk and milk quality analysis
U2

CO3 To investigate various extracellular enzyme producers in soil and to gain knowledge on
preparation of biofertilizers

CO4 Improve knowledge on plant pathogens

CO5 To acquire knowledge on preparation of probiotics and prebiotics

Unit Details No.of Course

Hours Objective
s
I 1. Physical, chemical, and microbiological assessment of water and 12
CO1
potability test forwater.
o Physical a – Color, pH,
o Chemical - alkalinity, acidity, DO, BOD, COD
o Microbiological – MPN index (Presumptive, Completed and
Confirmatorytest)
2. Study of air microflora by settle plate method.

II 3. Isolation and identification of bacteria and fungi from fruits and 12 CO2
vegetables
4. Direct microscopic count of milk.
5. Methylene blue reductase test and Resazurin test
6. Microbiological examination of milk by SPC.
III 7. Isolation of extracellular enzyme producers –Amylase, protease, 12 CO3
lipase
8. Microbiological assay of antibiotics by cup plate method and other
methods
9. Isolation of Rhizobium/ Azotobacter/ phosphate solubilizing
organisms
 117

10. Preparation of biofertilizers – Demonstration

IV 11. Study of plant pathogens- Tikka Disease, Red rot of sugarcane, 10 CO4
Citrus canker, Blight of paddy.
12. Study of fungi - Mucor,Curvularia, Alternaria, Rhizopus,
Aspergillus
V 13. Isolation of constituent flora of fermented milk. 14
CO5
14. Growth of probiotic LAB in broth, milk and whey.
15. Preparation of probiotic fermented milks like dahi, yoghurt, lassi
and whey drink.
16. Effect of prebiotics on the growth of LAB in milk and broth.
17. Survivability of probiotic organisms in fermented milks.
18. Antimicrobial potential of the functional dairy products.
Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Assess the microbial quality of water and relate the experimental PO1,
PO4,PO5,PO6,
results to the prescribed standards by the statutory bodies
PO7, PO8

CO2 Evaluate the quality of milk and enumerate bacteria in milk by PO5,PO6, PO7,
PO8
standard plate count method
CO3 Identify extracellular enzyme producing and nitrogen fixing PO1,PO8
microorganism form soil and to prepare a biofertilizer.
CO4 Identifyvarious plant pathogenic bacteria PO1
CO5 Synthesize probiotic fermented milks using microorganisms PO1,PO7,PO8

Text Books
1. Cappucino J and Sherman N.(2010). Microbiology: A Laboratory Manual. 9th Edition.
Pearson Education Limited.

2. Kannan. N. (1996). Laboratory manual in General Microbiology. Palani Publications.

3. R C Dubey and D K Maheswari.(2002). Practical Microbiology. S. Chand Publishing.

 118

4. Neelima Garg, K.L. Garg, K.G. Mukerji (2010).Laboratory Manual of Food Microbiology,
Wiley publication
5. Aneja, KR.(2010). Experiments in Microbiology, Plant pathology and Biotechnology.
New Age International (P) Limited.
References Books

1 Christon J. Hurst Editor in Chief, Ronald L. Crawford, Jay L. Garland, David A. Lipson,
Aaron L. Mills, Linda D. Stetzenbach (2007). Manual of Environmental Microbiology,
Third Edition,Wiley publication.
2 James G Cappucino and Natalie Sherman.(2016). Microbiology – A laboratory
manual. 4thEdition. The Benjamin publishing company, New York.
3 Marylynn V. Yates, Cindy H. Nakatsu, Robert V. Miller, Suresh D. Pillai 2016). Manual of
Environmental Microbiology, 4th Edition,ASM press.
4 Burns, Richard G (2005). Environmental MicrobiologyA Laboratory Manual, 2 nd
Edition .Lippincott Williams & Wilkins, Inc.
5 Ian Pepper, Charles Gerba, Jeffrey Brendecke (2004). Environmental Microbiology-A
laboratory manual, Elsevier.
Web Resources

1 https://micobenotes.com/fields-of-microbiology/

2 https://bio.libretexts.org
3 https://www.google.com
4 https://www.sfamjournals.onlinelibrary.wiley.com
5 https://www.degruyter.com
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
100 Marks
Total
Methods of Assessment

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between
various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
 119

Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or

Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

CO1 S M S S S S

CO2 M M M M

CO3 M S

CO4 M

CO5 M S S

ELECTIVE GENERIC /DISCIPLINE SPECIFIC ELECTIVE- VIII- PHARMACEUTICAL

MICROBIOLOGY

Subject Subject Name Category L T P S Credit Inst. Marks

Code s
Hour CI Ext Total
s A ern
al

22MBUG PHARMACEUTICAL ELECTI Y - - - 3 5 25 75 100

DE7 MICROBIOLOGY VE
GENERI
C
/DISCIPL
INE
SPECIFI
C
ELECTI
VE- VII-
Course Objectives

CO1 To provide the knowledge on basics of chemotherapy

 120

CO2 To learn the assays and testing methods of antibiotics.

CO3 To gain information about spoilage of pharmaceutical products

CO4 To provide the knowledge on drug discovery and clinical trials

CO5 To learn about regulations in pharmaceutical industry

Unit Details No.of Course

Hours Objective
s

I Introduction to Pharmaceutical microbiology: Ecology of 12 CO1

microorganisms in pharmaceutical industry: Atmosphere, water, skin
and respiratory flora of workers, raw materials, packaging, building
and equipments and their control measures; Design and layout of
sterile manufacturing.

II Microbial contamination and spoilage of pharmaceutical products: 10 CO2

Microbial aspects of pharmaceutical products; Sterilization of
pharmaceutical products: Heat, gaseous, radiation and filtration;
Contamination and Spoilage of Pharmaceutical products: sterile
injectable and non-injectable, ophthalmologic preparation, implants.

III Production of antibiotics: Production of antibacterial – Penicillin, 12 CO3

Tetracycline; antifungal – Griseofulvin, Amphotericin; antiparasitic
agents – Artemesin, Metronidazole; Semi-synthetic antibiotics and
anticancerous agents; Additional application of microorganisms in
pharmaceutical sciences: Enzymes- Streptokinase, Streptodornase, L-
asperginase and clinical dextrin; Immobilization procedures for
pharmaceutical applications (liposomes); Biosensors in
pharmaceuticals.

IV Production of immunological products and their quality control: 16 CO4

Vaccines - DNA vaccines, synthetic peptide vaccines, multivalent
vaccines; Vaccine clinical trials; Immunodiagnostics - immuno sera
 121

and immunoglobulin; Quality control in Pharmaceutical: In – Process

and Final Product Control; Sterility tests.

V Quality Assurance and Validation:Good Manufacturing Practices 10 CO5

(GMP) and Good Laboratory Practices (GLP) in pharmaceutical
industry; Regulatory aspects of quality control; Quality assurance and
quality management in pharmaceuticals – BIS (IS), ISI, ISO, WHO
and US certification.

Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes

CO1 PO1,PO10
Learn the basics of chemotherapy and action of antibiotics
CO2 PO7
Carry out the microbiological assay of antibiotics
CO3 Analyse Microbiological standardization of Pharmaceuticals PO5,PO8,PO10
,sterility testing of pharmaceutical
productsApplysterilization in pharmaceutical industry

CO4 Evaluate the process and develop new strategies for rational PO9,PO10
drug design

CO5 Learn the Regulatory guidelines in pharmaceuticals product. PO3,PO5

Text Books

1. Chand Pasha Kedernath. (2021). Text book of Pharmaceutical Microbiology. Ramnath

Publisher.

2. Hugo WB and Russell AD. (2004).Pharmaceutical Microbiology VII edition. Blackwell

Scientific Publication, Oxford.

3 Franklin,DJ. and Snow, GA. (2013). Biochemistry of antimicrobial action.Chapman& Hall.

4 Kuntal Das (2019). Pharmaceutical Microbiology, second edition, NiraliPrakashan.

 122

5 PriyatamaPowar, Shital Nimbargi, VaijayantiSapre (2020). Pharmaceutical Microbiology, I

edition, Technical publications.

References Books

1 Handa, S.S. and Kapoor, V.K. (2022) .Pharamcognosy.

4thEdition.VallabhPrakashanPublishers,New Delhi.

2 Kokate, C.K., Durohit, A.P. and Gokhale, S.R.,(2002). Pharmacognosy. 12thedition

NiraliPrakasham Publishers, Pune.
3 S. P. Vyas & V. K. Dixit.(2003). Pharmaceutical Biotechnology. CBS Publishers &
Distributors, New Delhi.
4 Wallis, T.E. (2005). Text book of Pharmacognosy. 5 th edition. CBS publishers and
distributors, New Delhi.
5 Garrod, L.P., Lambert, HP. And C’Grady, F. (1973). Antibiotics and Chemotherapy. (eds).
Churchill Livingstone.
Web Resources

1 https://www.pharmapproach.com/introduction-to-pharmaceutical-microbiology/

2 https://www.iptsalipur.org/wp-content/uploads/2020/08/BP303T_PMB_UNIT_I.pdf

3 https://www.pharmanotes.org/2021/11/pharmaceutical-microbiology-b-pharma.html

4 https://snscourseware.org/snscphs/notes.php?cw=CW_604b15c6313c5

5 https://www.thermofisher.com

Methods of Evaluation

Continuous Internal Assessment Test

Assignments
Internal
Evaluation Seminars 25 Marks

Attendance and Class Participation

External
Evaluation End Semester Examination 75 Marks

Total 100 Marks

Methods of Assessment
 123

Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /

Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)

Application
Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between
various ideas, Map knowledge
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or
Presentations

Mapping with Programme Outcomes

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M M

CO2 M

CO3 S M M

CO L M
4
CO5 L M
 124

Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CI Exter Total
s A nal
22MB ENTREPRENE ELECTIVE Y - - - 3 5 25 75 100
UGDE GENERIC /
URSHIP AND
8 DISCIPLIN
BIO-BUSINESS E
SPECIFIC
ELECTIVE
- VIII
Course Objectives

CO1 Understanding basic concepts in the area of entrepreneurship, the role and importance
of entrepreneurship for economic development
CO2 Developing personal creativity and entrepreneurial initiative, adopting the key steps in
the elaboration of business idea.
CO3 Understanding the stages of the entrepreneurial process and the resources needed for
the successful development of entrepreneurial ventures.
CO4 Explain the central components of successful business strategies in biotechnology, and
create a business plan.
CO5 Understand the various funding resources and develop as Entrepreneur
Unit Details No.of Course
Hours Objective
s
I Bio Entrepreneurship: Introduction to bio-business, SWOT 12 CO1
analysis of bio-business. Ownership, Development of
Entrepreneurship; Stages in entrepreneurial process;
Government schemes and funding. Small scale industries:
Definition; Characteristics; Need and rationale.
II Entrepreneurship Opportunity in Agricultural Biotechnology: 12 CO2
Business opportunity, Essential requirement, marketing,
strategies, schemes, challenges and scope-with case study on
Plant cell and tissue culture technique, polyhouse culture. Herbal
bulk drug production, Nutraceuticals, value added herbal
products. Bioethanol production using Agricultural waste, Algal
 125

source. Integration of system biology for agricultural

applications. Biosensor development in Agriculture
management.
III Entrepreneurship Opportunity in Industrial Biotechnology: 12 CO3
Business opportunity, Essential requirement, marketing
strategies, schemes, challenges, and scope- Pollution monitoring
and Bioremediation for Industrial pollutants. Integrated compost
production- microbe enriched compost. Bio pesticide/ insecticide
production. Biofertilizer. Single cell protein.
IV Therapeutic and Fermented products: Stem cell production, stem 12 CO4
cell bank, production of monoclonal/polyclonal antibodies,
secondary metabolite production – antibiotics, probiotic and
prebiotics.
V Project Management, Technology Management and Startup 12 CO5
Schemes: Building Biotech business challenges in Indian
context-biotech partners (BIRAC, DBT, Incubation centers.
etc.,), operational biotech parks in India. Indian Company act for
Bio business-schemes and subsidies. Project proposal
preparation, Successful start-ups-case study.
Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Describe and apply several entrepreneurial ideas and business PO1, PO2, PO3,
PO4, PO5, PO6,
theories in practical framework.
PO7, PO8, PO9,
PO10, PO11, PO12,
PO13, PO14
CO2 Analyse the business environment in order to identify business PO2, PO5, PO7,
PO8, PO10, PO12,
opportunities, identify the elements of success of entrepreneurial
PO14
ventures, evaluate the effectiveness of different entrepreneurial
strategies and interpret their own business plan.
 126

CO3 Express the mass production of microbial inoculants used as PO4, PO6, PO9,
PO11
Biofertilizers and Bioinsecticides in response with field
application and crop response.
CO4 Analyze the application and commercial production of PO5, PO6, PO9,
PO11
Monoclonal antibodies, Cytokines. TPH and teaching kits.
CO5 Integrate and apply knowledge of the regulation of PO2,PO7, PO8
biotechnology industries, utilize effective team work skills
within an effective management team with a common objective,
and gain effective team work skills, with an awareness of
cultural diversity and social inclusiveness.
Text Books

1. Craig Shimasaki. (2014). Biotechnology Entrepreneurship: Starting, Managing, and

Leading Biotech Companies. Academic Press.

2. Ashton Acton, O. (2012). Biological Pigments– Advances in Research and Application

Scholorly Editions: Atlanta, Georgia.
3. Jennifer Merritt, Jason Feifer (2018). Start Your Own Business, 7th edition,
Entrepreneur Press publisher.
4. Peter F. Drucker (2006). Innovation and Entrepreneurship. Harper Business publisher.
5. Leah Cannon (2017). How to Start a Life Science Company: A Comprehensive Guide
for First-Time Entrepreneurs. International Kindle paperwhite.
References Books

1 Crueger, W, and Crueger. A.(2000). Biotechnology: A Text Book of

Industrialmicrobiology, 2nd Edition, Sinauer Associates: Sunderland.Mass.
2 Paul S Teng. (2008). Bioscience Entrepreneurship in AsiaWorld Scientific Publishing
Company.
3 Charles E. Bamford, Garry D. Bruton (2015). ENTREPRENEURSHIP: The Art,
Science, and Process for Success, 2nd Edition, McGraw Hill publisher.
4 Yali Friedman (2014). Building Biotechnology: Biotechnology Business, Regulations,
Patents, Law, Policy and Science 4th Edition, Logos press publication.
5 Stephanie A. Wisner (2022). Building Backwards to Biotech: The Power of
Entrepreneurship to Drive Cutting-Edge Science to Market, International Kindle
paperwhite.
Web Resources
 127

1 https://www.bio-rad.com/webroot/web/pdf/lse/literature/Biobusiness.pdf
2 https://www.crg.eu/biobusiness-entrepreneurship
3 https://www.entrepreneur.com
4 https://www.birac.nic.in
5 https://www.springer.com
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations

Mapping with Programme Outcomes:

CO/PO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11

CO1 S S S S S S S S S S S

CO2 S M S S M

CO3

CO4 S S S S

CO5 S S S
 128

PROFESSIONAL COMPETENCY SKILL- MICROBIAL QUALITY CONTROL

Subject Subject Name Categor L T P S Cre Inst. Marks

Code y dits Hour CI Exter Tota
s A nal l
22MBUGPC MICROBIAL PROFE Y - - - 2 2 25 75 100
S SSIONA
QUALITY
L
CONTROL COMPE
TENCY
AND
SKILL
TESTING
Course Objectives

CO1 To understand the use of various advanced techniques for application in the field of
quality control and quality assurance.
CO2 To cultivate skills involved execution of microbiological techniques and to develop
the good laboratory practices.
CO3 To ensure the food safety regulations and its standards.
CO4 To acquire knowledge on laboratory testing, Control & safety process.
CO5 To analyze microbial standards to establish the quality of food products.

Unit Details No. of Course

Hours Objecti
ves
I Microbial quality control: definition, history and introduction. 12 CO1
Standard Methods involved in assessment of microbial quality
control. Q.A and Q.C definitions and importance. Traditional
Microbiological Quality Controlling methods: Sampling
methods, TVC, APC and serial dilution techniques. Good
laboratory practices, Good microbiological practices.
II Instruments associated in QC & QA: Principle involved, 12 CO2
working conditions, uses and precautions of Laminar Air Flow
(LAF), Autoclave, Incubator, pH meter, Colony counter, Hot
air oven, Centrifuges, colorimeter/ spectrophotometer, ELISA
and storage devices. Methodology of Disinfection,
 129

Autoclaving & Incineration.

III Culture media used in QC and QA: Design of specialized 12 CO3
media for identification of pathogens. Good laboratory
practices in culture media preparation: raw material, water,
pH.Uses of media.Enrichment culture technique, Detection of
specific microorganisms - on XLD agar, Salmonella Shigella
Agar, Mannitol salt agar, EMB agar, McConkey Agar,
Saboraud Agar.
IV Determining Microbes in Pharmaceutical Samples: Sterility 12 CO4
testing for pharmaceutical products, Bioburden, pyrogen test,
inprocess and final process control, safety and sterility test.
V HACCP for Food Safety and Microbial Standards: Hazard 12 CO5
analysis of critical control point (HACCP) - Principles, flow
diagrams, limitations. Microbial Standards for Different Foods
and Water – BIS standards for common foods and drinking
water.Ascertaining microbial quality of milk by MBRT, Rapid
detection methods of microbiological quality of milk at milk
collection centers.

Total 60

Course Outcomes

Course On completion of this course, students will;

Outcomes
CO1 Understand the theoretical assessment of microbial quality PO1, PO5, PO6,
PO9, PO10
methods and its good laboratory practices.
CO2 Describe the microbiological aspects of quality control of food PO1, PO4, PO5,
PO6
and pharmaceutical products.

CO3 Explain the identification of pathogenic microorganisms and PO1, PO3, PO5,
PO6, PO9
good laboratory practices.
CO4 Acquire the knowledge of different sterility test for the PO1, PO4, PO5,
 130

pharmaceutical products. PO6

CO5 Illustrate the safety concern management and regulations of PO1,PO3, PO4,
PO5, PO6, PO9,
food and pharmaceutical industry and learn the basic standard
PO10
methods and procedures for the microbiological analysis of
food.
Text Books

1 W.B.Hugo&A.D.Russell. (1998). Pharmaceutical Microbiology.6thEdition.

Blackwell scientific Publications.
2 Kulkarni A. K. Bewoor V. A. ()Quality Control,Wiley India Pvt. Ltd,
3 Chandrakant Kokare (2016). Pharmaceutical Microbiology, 1st Edition, Nirali
Publication.
4
Brown.M.R.W. (2017).Microbiological Quality Assurance
A Guide Towards Relevance and Reproducibility of Inocula,1st Edition. CRC
press.
5
Dev Raj Rakesh Sharma And V K Joshi (2011).Quality Control For Value Addition
In Food Processing, New India Publishing Agency.
References Books

1 Rosamund M. Baird, Norman A. Hodges, Stephen P. Denyer. (2000). Handbook of

Microbiological Quality Control in Pharmaceuticals and Medical Devices. 1st
Edition, CRC Press.
2 Konieczka, (2012). Quality Assurance and Quality Control in the Analytical
Chemical Laboratory A Practical Approach (Hb), Routledge, Taylor and Francis
group.
3 Singh Gajjar, Budhrani, Usman. (2021). Quality Control And Quality Assurance
(M.Pharm)SVikas And Company.
4 David Roesti, Marcel Goverde (2019). Pharmaceutical Microbiological Quality
Assurance and Control: Practical Guide for Non-Sterile Manufacturing, Wiley
publication.
5 Amihud Kramer Bernard A. Twigg (2017). Quality Control For The Food Industry
Fundamentals & Applications (Vol.1) 3rd Edition, MEDTEC publication.
Web Resources
1 https://www.study.com/microbiology-quality-control-testing-definition-procedures.
2 https://www.sigmaaldrich.com
 131

3 https://www.coursera.org
4 https://www.atcc.org
5 https://www.fao.org
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total
100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehen
overview
d (K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Analyze Problem-solving questions, Finish a procedure in many steps, Differentiate
(K4) between various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
Mapping with Programme Outcomes:

CO/ PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
PO
CO1 S S S S S
CO2 S M M M
CO3 S M S S M
CO4 S S M M
CO5 S S M S S S S
