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 Help

Help Dipper and Mabel find out the truth

about Vertebrate Animal Rules.
Join us on a journey of detective work
and mystery.
Have fun!
 Group 3

Adoremos
Aguila
Quilatan
Amigo
Dacasin
Faigmani
Montero
Solano
 Yanna as Mabel Jojie as Stan Apple as Wendy
Adie as Dipper
Adie as Dipper Yanna as Mabel Jojie as Stan Apple as Wendy
Adie as Dipper Apple as Wendy
Yanna as Mabel
Jojie as Stan
Adie as Dipper Jojie as Stan
Yanna as Mabel
Apple as Wendy
 CHaRADES!
CHaRADES!

Each group will pick a representative. We

will tell the representative which word will
he act for his/her members to guess! You’ll
be given 30 seconds to guess.
CHaRADES!
CHaRADES!
The word is...

CHaRADES!
CHaRADES!

FISH
FISH
CHaRADES!
CHaRADES!
The word is...

CHaRADES!
CHaRADES!

FROG
FISH
CHaRADES!
CHaRADES!
The word is...

CHaRADES!
CHaRADES!

HORSE
FISH
CHaRADES!
CHaRADES!
 The word is...

CHaRADES!
CHaRADES!

OSTRICH
FISH
 4Rs
Decode: one of the
is...

-.. .- -.-. .
.-. . .--. .
These rules were made with both the subject
researchers’ and the vertebrate animal’s
well-being and safety in mind.
Replace
r

Reduce
Refine
Respect
Replace
Vertebrate animals with invertebrates,
lower life forms, tissue/cell cultures
and/or computer simulations where
possible.
Replace
Reduce
The number of animals without
compromising statistical validity.
Replace
Reduce
Refine
The experimental protocol to minimize
pain or distress to the animals.
Replace
Reduce
Refine
Respect
Animals and their contribution to
research.
 RULES FOR ALL
VERTEBRATE ANIMAL
STUDIES
 RULES FOR ALL
VERTEBRATE ANIMAL
STUDIES

Vertebrate Animal Rules apply

to studies involving usage of
vertebrate animals.
Vertebrate animals, as covered
by these rules, are defined as:
 RULES FOR ALL
VERTEBRATE ANIMAL
STUDIES
1) All nonhuman vertebrates (including fish)
at hatching or birth.
2) Live nonhuman vertebrate mammalian
embryos or foetuses
3) Tadpoles
4) Bird and reptile eggs starting three days
(72 hours) prior to hatching
 Guide
Not all studies may be considered a
vertebrate animal study under some
conditions (must abide by rules set for a
tissue study)
Animals may be classified vertebrate
animals if rules stated earlier are applicable
(with exemptions due to different
developmental processes that vary between
different species)
All vertebrate animal studies must include a
research plan that includes:
a) Justification of why animals must be used,
including the reasons for the choice of species, the
source of animals and the number of animals to be
used; description, explanation, or identification of
alternatives to animal use that were considered, and
the reasons these alternatives were unacceptable;
explanation of the potential impact or contribution
this research may have on the broad fields of
biology or medicine.
b) Description of how the animals will be used.
Include methods and procedures, such as
experimental design and data analysis; description
of the procedures that will minimize the potential for
discomfort, distress, pain, and injury to the animals
during the course of experimentation; identification of
the species, strain, sex, age, weight, source and
number of animals proposed for use.
All vertebrate animal studies must be reviewed and
approved before experimentation begins. An Institutional
Animal Care and Use Committee, known as an IACUC, is the
institutional animal oversight review and approval body for
all animal studies at a Regulated Research Institution. The
local or affiliated fair SRC serves in this capacity for
vertebrate animal studies performed in a school, home or
field. Any SRC serving in this capacity must include a
veterinarian or an animal care provider with training
and/or experience in the species being studied.
 Students performing vertebrate animal
research must satisfy US federal law as well
as local, state, and country laws and
regulations of the jurisdiction in which
research is performed.
Research projects which cause more than momentary or
slight pain or distress are prohibited. Any illness or
unexpected weight loss must be investigated and a
veterinarian consulted to receive required medical care. This
investigation must be documented by the Qualified Scientist or
Designated Supervisor, who is qualified to determine the
illness, or by a veterinarian. If the illness or distress is caused
by the study, the experiment must be terminated immediately.
 No vertebrate animal deaths due to the
experimental procedures are permitted in
any group or subgroup.
a) Studies that are designed or
anticipated to cause vertebrate
animal death are prohibited.
b) Any death that occurs must be
investigated by a veterinarian, the
Qualified Scientist or the Designated
Supervisor who is qualified to determine if
the cause of death was incidental or due to
the experimental procedures. The project
must be suspended until the cause is
determined and then the results must be
documented in writing.
c) If death was the result of the
experimental procedure, the study must
be terminated, and the study will not
qualify for competition.
Let’s see what you got!
All animals must be monitored for signs of distress. Because
significant weight loss is one sign of stress, weight must be
recorded at least weekly with 15% being the maximum
permissible weight loss or growth retardation (compared to
controls) of any experimental or control animal. If weighing of
animals cannot be done in a fashion that is safe for both the
researcher and the animal, then an explanation and approval
by an SRC or IACUC needs to be included in the research
plan, as well as an alternative method(s) to address signs of
distress.
Additionally, body conditioning scoring
(BCS) systems are available for most
species of animals utilized in research and
agriculture and are an objective method for
assessing the overall health status of the
research subject, with or without weight
loss. A BCS system should be included in
the design of any study utilizing live
vertebrate animals and results regularly
recorded.
Students are prohibited from designing or
participating in an experiment associated
with the following types of studies on
vertebrate animals:
a) Induced toxicity studies with known toxic
substances that could cause pain, distress, or
death, including but not limited to alcohol,
acid rain, pesticides, or heavy metals or
studies with the intent to study toxic effects of
a substance on a vertebrate animal.
b) Behavioural experiments using
conditioning with aversive stimuli,
mother/infant separation or induced
helplessness.
c) Studies of pain.
d) Predator/vertebrate prey experiments.
Justification is required for an experimental design that
involves food or fluid restriction and must be appropriate to
the species. If the restriction exceeds 18 hours, the project
must be reviewed and approved by an IACUC and
conducted at a Regulated Research Institution (RRI).
Animals may not be captured from or released into the wild
without approval of authorised wildlife or other regulatory
officials. All appropriate methods and precautions must be
used to decrease stress. Fish may be obtained from the wild
only if the researcher releases the fish unharmed, has the
proper licence, and adheres to state, local and national
fishing laws and regulations. The use of electrofishing is
permissible only if conducted by a trained supervisor;
students are prohibited from performing electrofishing.
A Qualified Scientist or Designated
Supervisor must directly supervise all
research involving vertebrate animals,
except for observational studies.
After initial SRC approval, a student with
any proposed changes in the Research
Plan/Project Summary of the project must
repeat the approval process before
laboratory experimentation/data collection
resumes.
Hey! Isn’t the topic getting
quite boring nowadays?
There’s more to come so
why don’t we play a game?
LET’S GO!
https://www.canva.com/design/DAFusRmHZsk/RIi8j7TrT9K7N0WKYCFrBQ/view
Section A
 Section A
Vertebrate animal studies may be
conducted at a home, school, farm, ranch,
in the field, etc. This includes:
 Section A
1) Studies of animals in their natural environment.
2) Studies of animals in zoological parks.
3) Studies of livestock that use standard agricultural
practices.
4) Studies of fish that use standard aquaculture
practices.
 Section A
These projects must be reviewed and
approved by an SRC in which one member
is either a veterinarian and/or an animal
care provider/expert with training and/or
experience in the species being studied.
These projects must adhere to BOTH of the
following guidelines:
a) The research involves only agricultural,
behavioral, observational, or supplemental
nutritional studies on animals.
b) The research involves only non-invasive
and non-intrusive methods that do not
negatively affect an animal’s health or well-
being.
 Guide

All vertebrate animal studies that do

not meet the criteria in Section A
must be conducted in a Regulated
Research Institution (RRI).
 SECTION A
1. These projects must adhere to BOTH of the
following guidelines:
a) The research involves only agricultural,
behavioral, observational, or supplemental
nutritional studies on animals.
b) The research
Let’s involves
see only
what non-invasive
you got! and
non-intrusive methods that do not negatively
affect an animal’s health or well-being.
Animals must be treated kindly and cared for properly.
Animals must be housed in a clean, ventilated, comfortable
environment appropriate for the species. They must be given
a continuous, clean (uncontaminated) water and food supply.
Cages, pens and fish tanks must be cleaned frequently.
Proper care must be provided at all times, including
weekends, holidays, and vacation periods. Animals must be
observed daily to assess their health and well-being.
A Designated Supervisor is required to oversee the
daily husbandry of the animals. Any of the following
U.S. documents provide further guidance for animal
husbandry:
Federal Animal Welfare Regulation
Guide for the Care and Use of Laboratory
Animals
Guide for the Care and Use of Agricultural
Animals in
Agricultural Research and Teaching (Ag-Guide)
Quality Assurance Manuals (for the appropriate
species)
The Local or Affiliated Fair Scientific Review Committee
must determine if a veterinarian’s certification of the
research and animal husbandry plan is required. This
certification, as well as SRC approval, is required before
experimentation and is documented on Vertebrate Animal
Form 5A. A veterinarian must certify experiments that
involve supplemental nutrition, administration of
prescription drugs, and/or activities that would not be
ordinarily encountered in the animal’s daily life.
If an illness or emergency occurs, the affected animal(s)
must receive proper medical or nursing care that is
directed by a veterinarian. A student researcher must stop
experimentation if there is unexpected weight loss or death
in the experimental subjects. The experiment can only be
resumed if the cause of illness or death is not related to the
experimental procedures and if appropriate steps are
taken to eliminate the causal factors. If death is the result of
the experimental procedure, the study must be terminated,
and the study will not qualify for competition.
The final disposition of the animals must be
conducted in a responsible and ethical
manner, and must be described on
Vertebrate Animal Form 5A.
Euthanasia for tissue removal and/or
pathological analysis is not permitted for a
project conducted in a school/home/field
Site.
Livestock or fish raised for food using
standard agricultural/aquacultural
production practices may be euthanized by
a qualified adult for carcass evaluation.
The following forms are required:
a) Checklist for Adult Sponsor (1), Student Checklist
(1A), Research Plan/Project Summary, and Approval
Form (1B)
b) Vertebrate Animal Form (5A)
c) Qualified Scientist Form (2), when applicable
SECTION b
The Institutional Animal Care and Use Committee (IACUC)
or the comparable animal oversight committee must
approve all student research projects before
experimentation begins. Such research projects must be
conducted under the responsibility of a principal
investigator. The local and affiliated fair SRCs must also
review the project to certify that the research project
complies with ISEF Rules. This local and regional SRC
review should occur before experimentation begins, if
possible.
Student researchers are prohibited from
performing euthanasia. Euthanasia at the
end of experimentation for tissue removal
and/or pathological analysis is permitted.
All methods of euthanasia must adhere to
current American Veterinary Medical
Association (AVMA) Guidelines.
Research projects that cause more than
momentary or slight pain or distress to
vertebrate animals are prohibited unless
mitigated by IACUC-approved anesthetics,
analgesics, and/or tranquilizers.
Research in nutritional deficiency or research involving
substances or drugs of unknown effect is permitted to the
point that any clinical sign of distress is noted. In the case
that distress is observed, the project must be suspended
and measures must be taken to correct the deficiency or
drug effect. A project can only be resumed if appropriate
steps are taken to correct the causal factors.
5. The following forms are required:
a) _______________ (1), ________________________ (1A),
________________________________________________________________________ (1B)
b) ____________________________________________ (1C)
c) _____________________________________ (2)
d) _______________________________________ (5B)
e) _________________________________(6A) – for all studies involving
tissues and body fluids.
f) ___________________________________________________ (6B) – for all
studies involving tissues and body fluids.
5. The following forms are required:
a) Checklist for Adult Sponsor (1), Student Checklist (1A),
Research Plan/Project Summary, and Approval Form (1B)
b) Regulated Research Institution Form (1C)
c) Qualified Scientist Form (2)
d) Vertebrate Animal Form (5B)
e) PHBA Risk Assessment Form (6A) – for all studies involving
tissues and body fluids.
f) Human and Vertebrate Animal Tissue Form (6B) – for all
studies involving tissues and body fluids.
exempt
studies
 exempt
studies
Studies involving behavioral observations of animals are
exempt from prior SRC review if ALL of the following apply:
a) There is no interaction with the animals being observed,
b) There is no manipulation of the animal environment in any
way; and
c) The study meets all federal and state agriculture, fish,
game and wildlife laws and regulations.
HULAAN NYO KUNG SINO GUMAWA hehe
MULTIPLE
CHOICE
MULTIPLE CHOICE
6 . In rule 3 of Section A, who will determine if a
veterinarian’s certification of the research and animal
husbandry plan is required

A. Scientific Review Committee

B. Institutional Review Board
C. Institutional Animal Care & Use Committee
D. International Animal Care & Use Committee
MULTIPLE CHOICE
6 . In rule 3 of Section A, who will determine if a
veterinarian’s certification of the research and animal
husbandry plan is required

A. Scientific Review Committee

B. Institutional Review Board
C. Institutional Animal Care & Use Committee
D. International Animal Care & Use Committee
MULTIPLE CHOICE
6 . In rule 5 of Rules for All I. Veterinarian
Vertebrate Animal Studies, II. Qualified Scientist
if an animal death occurs, III. Designated Supervisor
who can investigate?

A. I only
B. II and III
C. 1, II and III
D. I and II
MODIFIED
TRUE OR FALSE

PERO with a twist....

 INSTRUCTIONS
Write FALSE if the BOLD statement is True;
If the BOLD statement is false, write TRUE and change
the BOLD statement to make it true

FORMAT
IF FALSE:

TRUE - CORRECT ANSWER

MODIFIED T OR F
7 . All vertebrate animal studies that do not meet the
criteria in Section B must be conducted in a Regulated
Research Institution (RRI).
8 . In Section B, the 3rd rule states that Research projects
that cause more than momentary or slight pain or distress
to vertebrate animals are prohibited unless mitigated by
SRC-Approved anesthetics, analgesics, and/or
tranquilizers.
9 . Exempt studies are studies involving behavioral
observations of animals are exempt from prior IACUC
review.
MODIFIED T OR F
8 . In Section B, the 3rd rule states that Research projects
that cause more than momentary or slight pain or distress
to vertebrate animals are prohibited unless mitigated by
SRC-Approved anesthetics, analgesics, and/or
tranquilizers.

9 . Exempt studies are studies involving behavioral

observations of animals are exempt from prior IACUC
review.
MODIFIED T OR F
10 . In Replace, it states that Vertebrate animals with
invertebrates, higher life forms, tissue/cell cultures
and/or computer simulations where possible.
11 . In Rules for all Vertebrate Animal Studies, the 6th rule states
that Because significant weight loss is one sign of stress, weight
must be recorded at least weekly with 15% being the maximum
permissible weight loss or growth retardation

12 . In Rules for all Vertebrate Animal Studies, the 8th

rule states that If therestriction exceeds 24 hours, the
project must be reviewed and approved by an IACUC
and conducted at a Regulated Research Institution (RRI)
FILL IN THE
B L A N K S
FILL IN THE BLANKS

13 . AVMA stands for ____________________ ___________________

________________ ___________________.
14 . In Rules for all Vertebrate Animal Studies, the 8th rule
states that ________________ is required for an experimental
design that involves food or fluid restriction and must be
appropriate to the species.
15 . Vertebrate Form (6B) is needed for all studies involving
________________ and ____________________.
FILL IN THE BLANKS
16 . Livestock or fish raised for food using standard
agricultural/aquacultural production practices may be
________________ by a qualified adult for carcass evaluation.
17 . In Section A, the 5th rule states that the final disposition of
the animals must be conducted in a responsible and ethical
manner, and must be described on _____________________________.
18 . In Section A, the 1st rule states that The Institutional
Animal Care and Use Committee (IACUC) or the
________________________________ must approve all student
research projects before experimentation begins.
ENUMERATION
ENUMERATION
19 . Give us the 3rd rule of Vertebrate Animal Rules,
SECTION A.

20 . Give us the 11th rule of Vertebrate Animal Rules for

ALL STUDIES.
Group 3

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