Cambridge International AS & A Level: Biology 9700/13

Download as pdf or txt
Download as pdf or txt
You are on page 1of 20

Cambridge International AS & A Level

BIOLOGY 9700/13
Paper 1 Multiple Choice May/June 2023
1 hour 15 minutes

You must answer on the multiple choice answer sheet.


*8864591307*

You will need: Multiple choice answer sheet


Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
• There are forty questions on this paper. Answer all questions.
• For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
• Follow the instructions on the multiple choice answer sheet.
• Write in soft pencil.
• Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
• Do not use correction fluid.
• Do not write on any bar codes.
• You may use a calculator.

INFORMATION
• The total mark for this paper is 40.
• Each correct answer will score one mark.
• Any rough working should be done on this question paper.

This document has 20 pages. Any blank pages are indicated.

IB23 06_9700_13/5RP
© UCLES 2023 [Turn over
2

1 Which feature is visible with a light microscope using a natural light source?

A DNA molecule of diameter 2 nm

B Paramecium cell of diameter 200 µm


C phospholipid bilayer of width 8 nm
D ribosome of diameter 20 nm

2 The electron micrograph shows a structure found in the cytoplasm of an animal cell.

Which biological molecules are found in this structure?

1 nucleic acids
2 proteins
3 phospholipids

A 1 and 3 B 1 only C 2 and 3 D 2 only

3 Which cell structures contain nucleic acid?

1 cytoplasm
2 lysosomes
3 mitochondria

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 3 only

4 Mitochondria are thought to have evolved from prokaryotic cells that were ingested by an
ancestral cell.

Which feature have prokaryotes lost during their evolution into mitochondria?

A cell wall
B circular chromosome
C endoplasmic reticulum
D ribosomes

© UCLES 2023 9700/13/M/J/23


3

5 Which polymers are present in all viruses, all prokaryotes and all eukaryotes?

1 polynucleotides
2 polypeptides
3 polysaccharides

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

6 How many of the listed structures typically contain genetic material that has telomeres?

• bacterial cell

• chloroplast

• mitochondrion

• nucleus

A 1 B 2 C 3 D 4

7 Steps 1, 2, 3 and 4 are used to test for a non-reducing sugar.

1 Put 5 cm3 of solution into a test-tube.


2 Add a few drops of acid.
3 Neutralise with alkali.
4 Add 6 cm3 of Benedict’s solution.

When is the solution heated or boiled?

A between steps 1 and 2


B between steps 2 and 3, and after step 4
C between steps 2 and 3 only
D after step 4 only

8 Which features contribute to the function of a cellulose molecule?

1 Long chains of glucose molecules coil into a helix.


2 Many hydrogen bonds form between adjacent chains.
3 It is insoluble in water.

A 1, 2 and 3 B 1 and 3 only C 2 and 3 only D 2 only

© UCLES 2023 9700/13/M/J/23 [Turn over


4

9 What correctly describes triglycerides?

A non-polar molecules that are soluble in ethanol


B non-polar molecules that are soluble in water
C polar molecules that are soluble in ethanol
D polar molecules that are soluble in water

10 Cocoa butter contains three different triglycerides. These triglycerides are made from the fatty
acids:

• oleic acid (O)

• palmitic acid (P)

• stearic acid (S).

The three triglycerides found in cocoa butter are POS, SOS and POP.

The chemical structure of the triglyceride POS is shown next to a diagrammatic representation of
POS.

H O H H H H H H H H H H H H H H H

H C O C C C C C C C C C C C C C C C C H G P

H H H H H H H H H H H H H H H L
Y
O H H H H H H H H H H H H H H H H H
C
H C O C C C C C C C C C C C C C C C C C C H O
E
H H H H H H H H H H H H H H H
R
O H H H H H H H H H H H H H H H H H O

H C O C C C C C C C C C C C C C C C C C C H L S

H H H H H H H H H H H H H H H H H H

Which statement is correct?

A Triglyceride POP contains two unsaturated fatty acids joined by ester bonds to glycerol.
B Triglyceride POS contains two less carbon atoms than triglyceride POP.
C Triglyceride SOS contains four more carbon atoms than triglyceride POP.
D Triglyceride SOS contains two saturated fatty acids joined by hydrolysis to glycerol.

© UCLES 2023 9700/13/M/J/23


5

11 Which molecules contain at least four double bonds?

unsaturated
triglyceride

C A
D

haemoglobin collagen
B

12 The diagram shows the amino acid glutamic acid.

COOH

H C CH2 CH2 COOH

NH2

What is the R group for glutamic acid?

A NH2
B H
C COOH
D CH2 CH2 COOH

© UCLES 2023 9700/13/M/J/23 [Turn over


6

13 The diagram shows three interactions that hold protein molecules in shape.

N
H
S NH3+
interaction 1 S
O
interaction 3
C
COO–

S
S
interaction 2

Which row identifies these interactions?

interaction 1 interaction 2 interaction 3

A hydrogen bond disulfide bond hydrophobic interaction


B hydrogen bond covalent bond ionic bond
C hydrophobic interaction ionic bond hydrogen bond
D ionic bond disulfide bond peptide bond

14 Some animals produce antimicrobial proteins which protect them from pathogens. These proteins
could be used to kill human pathogens, however when used as a medicine they are broken down
by protein-digesting enzymes.

Replacing one of the amino acids found in the protein with an amino acid that had been
synthesised in the laboratory resulted in a modified protein that was not broken down.

What could explain why this modified protein was not broken down by the protein-digesting
enzymes?

1 The modified protein has a different tertiary structure to the original protein.
2 The modified protein is not complementary in shape to the enzyme’s active site.
3 The modified protein is unable to induce a fit with the protein-digesting enzyme.

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

© UCLES 2023 9700/13/M/J/23


7

15 A student investigated the effect of substrate concentration on the rate of an enzyme-catalysed


reaction. A graph was plotted to show the relationship between these two variables. The
student was asked to take readings from the graph that could be used to determine the
Michaelis–Menten constant, Km, for this enzyme.

J, K, L and M show points read from the graphs which the student could use to determine the
value of Km.

J
rate of rate of
reaction reaction

0 0
0 0 K
concentration concentration
of substrate of substrate

rate of rate of
reaction reaction
L

0 0
0 0 M
concentration concentration
of substrate of substrate

Which two readings must the student use to determine the value of Km?

A J and K B J and M C K and L D L and M

© UCLES 2023 9700/13/M/J/23 [Turn over


8

16 The end-product of a metabolic pathway can act as a competitive inhibitor. This is called
end-product inhibition and allows a cell to control a metabolic pathway.

The diagram shows a metabolic pathway where the end-product could act as an inhibitor of
enzyme W.

substrate

enzyme W

intermediate 1

enzyme X

intermediate 2

inhibition enzyme Y

intermediate 3

enzyme Z

end-product

What would be the effect if enzyme Z was inhibited by the end-product instead of enzyme W?

quantity of quantity of
intermediate 1 end-product

A increase decrease
B increase unchanged
C decrease decrease
D decrease unchanged

© UCLES 2023 9700/13/M/J/23


9

17 Which row shows the distribution of cholesterol and the carbohydrate chains of glycolipids and
glycoproteins in a cell surface membrane?

carbohydrate chains carbohydrate chains


cholesterol
of glycolipids of glycoproteins
A between phospholipid mainly on the mainly on the
heads only outer surface inner surface
B between phospholipid mainly on the mainly on the
heads only inner surface outer surface
C between phospholipids mainly on the mainly on the
inner surface inner surface
D between phospholipids mainly on the mainly on the
outer surface outer surface

18 Which row correctly describes all the possible relative concentrations of a substance when the
substance is moved by endocytosis or exocytosis?

endocytosis exocytosis
A concentrations equal concentrations equal
B concentrations equal, concentrations equal,
greater inside or greater inside or
greater outside greater outside

C concentrations equal concentrations equal


or greater outside or lower outside
D concentrations equal concentrations equal
or lower outside or greater outside

19 Which statement about simple diffusion is correct?

A It requires specific molecules in the cell surface membrane.


B It is a passive mode of transporting substances.
C It always requires a membrane for transport of substances.
D It only happens in the cells of prokaryotes and eukaryotes.

© UCLES 2023 9700/13/M/J/23 [Turn over


10

20 Plant cells with the same water potential in their cytoplasm were each put into one of three
different concentrations of sugar solution, 10%, 5% and 2.5%.

The cells were left for 50 minutes and then observed using a light microscope.

cell X cell Y cell Z


vacuole

Which statements are correct?

1 Cell Y had a lower water potential than the sugar solution it was put into.
2 Cell Z was put into the 10% sugar solution.
3 Cell Z had a less negative water potential than the sugar solution it was put into.

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

21 Which row describes some properties of stem cells?

able to divide
able to
by mitosis to able to repair
differentiate into
produce more damaged cells
specialised cells
stem cells

A    key
B    = is a property
C    = is not a property
D   

© UCLES 2023 9700/13/M/J/23


11

22 The mitotic index is a measure of the proportion of cells that are undergoing mitosis in an area of
tissue. It is calculated using the formula shown.

mitotic index = (number of cells undergoing mitosis ÷ total number of cells) × 100

A scientist calculated the mitotic index of areas of onion root at different distances from the tip of
the root.

The results are shown.

14

12

10

8
mitotic
index
6

0
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0

distance from tip of root / mm

Which statement is correct?

A No cell division occurs further than 1.4 mm from the tip of the root.
B The rate of cell division decreases as the distance from the root tip decreases.
C Most of the cells undergoing cell division are closer to the tip of the root.
D For a sample of 200 cells 0.2 mm from the tip of the root, 6 would be undergoing mitosis.

23 Which molecules make up the structure of ATP?

1 adenine
2 thymine
3 deoxyribose
4 phosphate
5 ribose

A 1, 2 and 3 B 1, 3 and 4 C 1, 4 and 5 D 2, 4 and 5

© UCLES 2023 9700/13/M/J/23 [Turn over


12

24 A short piece of DNA, 19 base pairs long, was analysed to find the number of nucleotide bases
in each of the polynucleotide strands. Some of the results are shown.

number of nucleotide bases


A C G T

strand 1 4
strand 2 7 5

How many cytosines were in strand 1?

A 2 B 3 C 5 D 7

25 Which statement about the role of DNA polymerase in the process of semi-conservative
replication of DNA is correct?

A DNA polymerase forms the hydrogen bonds between complementary base pairs.
B DNA polymerase moves along the lagging strand in the 3′ to 5′ direction.
C DNA polymerase joins new bases to the leading strand only.
D DNA polymerase moves along leading and lagging strands in the 5′ to 3′ direction.

26 The diagram shows the process of translation occurring at a ribosome.

ribosome

S
C A U U C G C U A A U G

What is the base sequence at S?

A CUA B CAT C GAU D GAT

© UCLES 2023 9700/13/M/J/23


13

27 Which row correctly explains how the structures of phloem sieve tube elements and xylem vessel
elements are related to their functions as transport systems?

phloem sieve tube elements xylem vessel elements

A contain mitochondria to make ATP cell walls are thickened and


for the active loading of sucrose contain lignin which makes
into the phloem xylem vessels waterproof

B contain no nucleus so that end walls between cells are


dissolved solutes can move easily perforated so that water can move
through cells easily through xylem vessels

C end walls between cells are lignin in cell walls gives strength so
perforated so that dissolved solutes that xylem vessels do not collapse
can move between cells due to transpiration pull

D no end walls between cells so that contain no cytoplasm so that a


sucrose solution can move up and continuous column of water can
down the phloem move up the xylem vessels

28 Which component of plants is used by the apoplast pathway as water is moved from the soil to
the xylem?

A Casparian strip
B cellulose
C endodermis
D suberin

29 Which statements correctly describe transport pathways in dicotyledonous plants?

1 In the symplast pathway, water may move through intercellular spaces.


2 The symplast pathway may be blocked by the tonoplast.
3 In the apoplast pathway, water does not move through plasmodesmata.
4 The apoplast pathway may be blocked by the Casparian strip.

A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4

30 Plants, such as the tobacco plant, retain very little of the water they take in and the volume of
water lost during transpiration is very high. This is because these plants can only absorb
carbon dioxide through open stomata.

Plants use carbon dioxide to synthesise glucose molecules. It is estimated that 400 molecules of
water are lost for each carbon dioxide molecule gained.

How many water molecules are lost for a plant to synthesise one molecule of glucose?

A 800 B 1200 C 2000 D 2400

© UCLES 2023 9700/13/M/J/23 [Turn over


14

31 Which changes occur as sucrose is transferred from leaves into phloem sieve tubes to be
transported to a sink?

water potential volume of liquid


in phloem sieve in phloem
tubes becomes sieve tubes

A less negative decreases


B less negative increases
C more negative decreases
D more negative increases

32 The graph shown does not have any axis labels.

0
0

Which row shows appropriate labels for the axes that would explain mass flow in phloem?

x-axis y-axis

A distance from sink / m hydrostatic pressure / kPa


B hydrostatic pressure / kPa distance from sink / m
C distance from source / m hydrostatic pressure / kPa
D hydrostatic pressure / kPa distance from source / m

33 What is present in the blood in human veins?

1 chloride ions
2 carbonic anhydrase
3 oxyhaemoglobin

A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

© UCLES 2023 9700/13/M/J/23


15

34 The photomicrograph shows two blood vessels P and Q.

Both these blood vessels are part of a network which transports blood around the body.

Which row is correct for blood vessel P and blood vessel Q?

blood vessel P blood vessel Q

A carries blood into arterioles carries blood into arterioles


B carries blood into arterioles carries blood from venules
C carries blood from venules carries blood into arterioles
D carries blood from venules carries blood from venules

© UCLES 2023 9700/13/M/J/23 [Turn over


16

35 The diagram shows a blood capillary and the tissue fluid which surrounds it.

tissue fluid
W X

arteriole venule
Z blood capillary Y
end end

tissue fluid

Tissue fluid is formed when fluid and solutes from blood plasma pass through tiny gaps in the
capillary wall. Most tissue fluid is then returned to the blood in the capillary.

Which pressures will be needed at points W, X, Y and Z so that this system can function?

pressure at W pressure at X pressure at Y pressure at Z


/ mm Hg / mm Hg / mm Hg / mm Hg

A 18 26 36 26
B 26 26 18 36
C 26 36 26 18
D 36 18 26 26

36 What happens during ventricular systole in a mammalian heart?

A Aortic pressure increases.


B Atrioventricular valves open.
C Semilunar valves close.
D Ventricular pressure decreases.

© UCLES 2023 9700/13/M/J/23


17

37 The photomicrograph shows a part of the human gas exchange system with one tissue
labelled P.

Which row is correct?

part of gas
function of tissue P
exchange system

A bronchus regulates the amount of air reaching the lungs


B bronchus provides support and prevents collapse
C bronchiole regulates the amount of air reaching the lungs
D bronchiole provides support and prevents collapse

38 How many times must a molecule of oxygen pass through a cell surface membrane as it diffuses
from the airspace inside an alveolus, through a cell in the capillary wall, to bind to a molecule of
haemoglobin?

A 3 B 4 C 5 D 10

39 A student wrote four statements about cholera.

Which statement is not correct?

A It can be controlled by vaccination.


B It is caused by bacteria spread by ingestion.
C HIV/AIDS increases the risk of infection.
D It may be transmitted by animal vectors.

© UCLES 2023 9700/13/M/J/23 [Turn over


18

40 Monoclonal antibodies are produced using the hybridoma method.

What are removed from the spleen of a mouse to produce monoclonal antibodies?

A antigens
B clones
C lymphocytes
D myeloma cells

© UCLES 2023 9700/13/M/J/23


19

BLANK PAGE

© UCLES 2023 9700/13/M/J/23


20

BLANK PAGE

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.

To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.

Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.

© UCLES 2023 9700/13/M/J/23

You might also like