Cambridge International AS & A Level: Biology 9700/13
Cambridge International AS & A Level: Biology 9700/13
Cambridge International AS & A Level: Biology 9700/13
BIOLOGY 9700/13
Paper 1 Multiple Choice May/June 2023
1 hour 15 minutes
INSTRUCTIONS
• There are forty questions on this paper. Answer all questions.
• For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
• Follow the instructions on the multiple choice answer sheet.
• Write in soft pencil.
• Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
• Do not use correction fluid.
• Do not write on any bar codes.
• You may use a calculator.
INFORMATION
• The total mark for this paper is 40.
• Each correct answer will score one mark.
• Any rough working should be done on this question paper.
IB23 06_9700_13/5RP
© UCLES 2023 [Turn over
2
1 Which feature is visible with a light microscope using a natural light source?
2 The electron micrograph shows a structure found in the cytoplasm of an animal cell.
1 nucleic acids
2 proteins
3 phospholipids
1 cytoplasm
2 lysosomes
3 mitochondria
4 Mitochondria are thought to have evolved from prokaryotic cells that were ingested by an
ancestral cell.
Which feature have prokaryotes lost during their evolution into mitochondria?
A cell wall
B circular chromosome
C endoplasmic reticulum
D ribosomes
5 Which polymers are present in all viruses, all prokaryotes and all eukaryotes?
1 polynucleotides
2 polypeptides
3 polysaccharides
6 How many of the listed structures typically contain genetic material that has telomeres?
• bacterial cell
• chloroplast
• mitochondrion
• nucleus
A 1 B 2 C 3 D 4
10 Cocoa butter contains three different triglycerides. These triglycerides are made from the fatty
acids:
The three triglycerides found in cocoa butter are POS, SOS and POP.
The chemical structure of the triglyceride POS is shown next to a diagrammatic representation of
POS.
H O H H H H H H H H H H H H H H H
H C O C C C C C C C C C C C C C C C C H G P
H H H H H H H H H H H H H H H L
Y
O H H H H H H H H H H H H H H H H H
C
H C O C C C C C C C C C C C C C C C C C C H O
E
H H H H H H H H H H H H H H H
R
O H H H H H H H H H H H H H H H H H O
H C O C C C C C C C C C C C C C C C C C C H L S
H H H H H H H H H H H H H H H H H H
A Triglyceride POP contains two unsaturated fatty acids joined by ester bonds to glycerol.
B Triglyceride POS contains two less carbon atoms than triglyceride POP.
C Triglyceride SOS contains four more carbon atoms than triglyceride POP.
D Triglyceride SOS contains two saturated fatty acids joined by hydrolysis to glycerol.
unsaturated
triglyceride
C A
D
haemoglobin collagen
B
COOH
NH2
A NH2
B H
C COOH
D CH2 CH2 COOH
13 The diagram shows three interactions that hold protein molecules in shape.
N
H
S NH3+
interaction 1 S
O
interaction 3
C
COO–
S
S
interaction 2
14 Some animals produce antimicrobial proteins which protect them from pathogens. These proteins
could be used to kill human pathogens, however when used as a medicine they are broken down
by protein-digesting enzymes.
Replacing one of the amino acids found in the protein with an amino acid that had been
synthesised in the laboratory resulted in a modified protein that was not broken down.
What could explain why this modified protein was not broken down by the protein-digesting
enzymes?
1 The modified protein has a different tertiary structure to the original protein.
2 The modified protein is not complementary in shape to the enzyme’s active site.
3 The modified protein is unable to induce a fit with the protein-digesting enzyme.
J, K, L and M show points read from the graphs which the student could use to determine the
value of Km.
J
rate of rate of
reaction reaction
0 0
0 0 K
concentration concentration
of substrate of substrate
rate of rate of
reaction reaction
L
0 0
0 0 M
concentration concentration
of substrate of substrate
Which two readings must the student use to determine the value of Km?
16 The end-product of a metabolic pathway can act as a competitive inhibitor. This is called
end-product inhibition and allows a cell to control a metabolic pathway.
The diagram shows a metabolic pathway where the end-product could act as an inhibitor of
enzyme W.
substrate
enzyme W
intermediate 1
enzyme X
intermediate 2
inhibition enzyme Y
intermediate 3
enzyme Z
end-product
What would be the effect if enzyme Z was inhibited by the end-product instead of enzyme W?
quantity of quantity of
intermediate 1 end-product
A increase decrease
B increase unchanged
C decrease decrease
D decrease unchanged
17 Which row shows the distribution of cholesterol and the carbohydrate chains of glycolipids and
glycoproteins in a cell surface membrane?
18 Which row correctly describes all the possible relative concentrations of a substance when the
substance is moved by endocytosis or exocytosis?
endocytosis exocytosis
A concentrations equal concentrations equal
B concentrations equal, concentrations equal,
greater inside or greater inside or
greater outside greater outside
20 Plant cells with the same water potential in their cytoplasm were each put into one of three
different concentrations of sugar solution, 10%, 5% and 2.5%.
The cells were left for 50 minutes and then observed using a light microscope.
1 Cell Y had a lower water potential than the sugar solution it was put into.
2 Cell Z was put into the 10% sugar solution.
3 Cell Z had a less negative water potential than the sugar solution it was put into.
able to divide
able to
by mitosis to able to repair
differentiate into
produce more damaged cells
specialised cells
stem cells
A key
B = is a property
C = is not a property
D
22 The mitotic index is a measure of the proportion of cells that are undergoing mitosis in an area of
tissue. It is calculated using the formula shown.
mitotic index = (number of cells undergoing mitosis ÷ total number of cells) × 100
A scientist calculated the mitotic index of areas of onion root at different distances from the tip of
the root.
14
12
10
8
mitotic
index
6
0
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0
A No cell division occurs further than 1.4 mm from the tip of the root.
B The rate of cell division decreases as the distance from the root tip decreases.
C Most of the cells undergoing cell division are closer to the tip of the root.
D For a sample of 200 cells 0.2 mm from the tip of the root, 6 would be undergoing mitosis.
1 adenine
2 thymine
3 deoxyribose
4 phosphate
5 ribose
24 A short piece of DNA, 19 base pairs long, was analysed to find the number of nucleotide bases
in each of the polynucleotide strands. Some of the results are shown.
strand 1 4
strand 2 7 5
A 2 B 3 C 5 D 7
25 Which statement about the role of DNA polymerase in the process of semi-conservative
replication of DNA is correct?
A DNA polymerase forms the hydrogen bonds between complementary base pairs.
B DNA polymerase moves along the lagging strand in the 3′ to 5′ direction.
C DNA polymerase joins new bases to the leading strand only.
D DNA polymerase moves along leading and lagging strands in the 5′ to 3′ direction.
ribosome
S
C A U U C G C U A A U G
27 Which row correctly explains how the structures of phloem sieve tube elements and xylem vessel
elements are related to their functions as transport systems?
C end walls between cells are lignin in cell walls gives strength so
perforated so that dissolved solutes that xylem vessels do not collapse
can move between cells due to transpiration pull
28 Which component of plants is used by the apoplast pathway as water is moved from the soil to
the xylem?
A Casparian strip
B cellulose
C endodermis
D suberin
30 Plants, such as the tobacco plant, retain very little of the water they take in and the volume of
water lost during transpiration is very high. This is because these plants can only absorb
carbon dioxide through open stomata.
Plants use carbon dioxide to synthesise glucose molecules. It is estimated that 400 molecules of
water are lost for each carbon dioxide molecule gained.
How many water molecules are lost for a plant to synthesise one molecule of glucose?
31 Which changes occur as sucrose is transferred from leaves into phloem sieve tubes to be
transported to a sink?
0
0
Which row shows appropriate labels for the axes that would explain mass flow in phloem?
x-axis y-axis
1 chloride ions
2 carbonic anhydrase
3 oxyhaemoglobin
Both these blood vessels are part of a network which transports blood around the body.
35 The diagram shows a blood capillary and the tissue fluid which surrounds it.
tissue fluid
W X
arteriole venule
Z blood capillary Y
end end
tissue fluid
Tissue fluid is formed when fluid and solutes from blood plasma pass through tiny gaps in the
capillary wall. Most tissue fluid is then returned to the blood in the capillary.
Which pressures will be needed at points W, X, Y and Z so that this system can function?
A 18 26 36 26
B 26 26 18 36
C 26 36 26 18
D 36 18 26 26
37 The photomicrograph shows a part of the human gas exchange system with one tissue
labelled P.
part of gas
function of tissue P
exchange system
38 How many times must a molecule of oxygen pass through a cell surface membrane as it diffuses
from the airspace inside an alveolus, through a cell in the capillary wall, to bind to a molecule of
haemoglobin?
A 3 B 4 C 5 D 10
What are removed from the spleen of a mouse to produce monoclonal antibodies?
A antigens
B clones
C lymphocytes
D myeloma cells
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