Plesiomonas shigelloides

Citrobacter Klebsiella Enterobacter Serratia Proteus Providencia

Cronobacter sakazakii

Pantoea agglomerans

Yersinia enterocolitica
Ewingella americana

Morganella morganii
(was Enterobacter)
Edwardsiella tarda

C. koseri (formerly
Escherichia coli

Shigella sonnei

K. pneumoniae

S. marcescens
Other Shigella

E. aerogenes
S. enteritidis

S. odorifera
*oxidase +

P. mirabilis
K. oxytoxa

E. cloacae

P. vulgaris
C. freundii

P. rettgeri
C. braakii

P. stuartii
biotype 2
diversus)
H. alvei

S. typhi
Indole + – – + – V – – + – –(v) + – + – – – –(v) – V + – + + + V
Methyl red + + –(v) V + + + + + + + + V –(v) – – – V V +(v) + + + + + +
Voges Proskauer – + +(v) – – – – – – – – – + + + + + +(v) + + – V – – – –
Simmons citrate – + + – – – + – – + +(v) + + + + + + V + + –(v) +(v) – + + –
Hydrogen Sulfide (TSI) – – – – – – +(v) +w + + +(v) – – – – – – – – – + + – – – –
Urea – – – – – – – – – –(v) –(v) +(v) + + +(v) – – –(v) –(v) – + + + + –(v) +
Motility V +(v) + + – – + + + + + + – – + + + + + + + + V + +(v) –
Lysine decarboxylase +(v) – + + – – + + + – – – + + – + – – + + – – – – – –
Arginine dihydrolase –(v) – – + – V +(v) – – +(v) + + – – + – + – – – – – – – – –
Ornithine decarboxylase +(v) – + + + – + – + – + + – – + + + – + – – + + – – +
Phenylalanine deaminase – – – – – – – – – – – – – – – – +(v) –(v) – – + + + + + –
Gas from D–glucose + – + – – – + – + + + + + + + + + –(v) – – + + + – – –
Lactose + +(v) – V – – – – – +(v) + V + + + + + –(v) – + – – – – – –
Sucrose V – – – – – – – – +(v) – –(v) + + + + + +(v) + – + – – – V +
D–Mannitol + + + – + + + + – + + + + + + + + + + + – – – + –(v) +
Adonitol – – – – – – – – – – – + + + –(v) + – – –(v) +(v) – – – + – –
Inositol – – – + – – – – – – – – + + –(v) + + –(v) V + – – – + + –
D–Sorbitol +(v) – – – – V + + – + + + + + + + – –(v) + + – – – – – +

CHAPTER 19 Enterobacteriaceae
L–Arabinose + – + – + V + – – + + + + + + + + + – + – – – – – +
Raffinose V – – – – V – – – –(v) _ – + + + + + –(v) – – – – – – – –
L–Rhamnose – –(v) + – +(v) –(v) + – – + + + + + + + + + – + – – – +(v) – –
KCN, growth in – – + – – – – – – + + – + + + + + –(v) + – + + + + + –
Gelatin (22°C) – – – – – – – – – – – – – – – – – – –(v) + + + – – – –
DNase – – – – – – – – – – – – – – – – – – + – – – – – – –

• Figure 19-4 Biochemical differentiation of representative Enterobacteriaceae. 1(v), greater probability

for positive reaction .50%; 2(v), greater probability for negative reaction .50%; (1), positive .80%; (2),
negative .80%; V, variability can be equally either positive or negative. The pink squares indicate a pattern
useful for preliminary recognition. The green squares indicate a key characteristic for biochemical identifi-
cation.

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