DNA Structure

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DNA Structure and Replication

How is genetic information stored and copied?

Why?
Deoxyribonucleic acid or DNA is the molecule of heredity. It contains the genetic blueprint for life. For
organisms to grow and repair damaged cells, each cell must be capable of accurately copying itself. So how
does the structure of DNA allow it to copy itself so accurately?

Model 1 – The Structure of DNA


Ladder Model of DNA Helix Model of DNA
Nucleotide
C G
Phosphate

T A
Deoxyribose Nitrogen-
sugar containing
A T
base

Nitrogen Bases

Adenine
G C
Thymine
CG

I
Guanine

Cytosine

1. Refer to the diagram in Model 1.


a. What are the three parts of a nucleotide?
sugar t phosphate base
b. What kind of sugar is found in a nucleotide?
deoxyribose
c. Which nucleotide component contains nitrogen?

bases CAT461
d. Name the four nitrogen bases shown in Model 1.
Adenine
A ThymineCtl CytosineCal Guanine
2. DNA is often drawn in a “ladder model.” Locate this drawing in Model 1.
a. Circle a single nucleotide on each side of the ladder model of DNA.

DNA Structure and Replication 1


b. What part(s) of the nucleotides make up the rungs of the “ladder”?

bases
c. What parts of the nucleotides make up the sides (backbone) of the “ladder”?

sugar phosphate
d. Look at the bottom and top of the “ladder” in Model 1. Are the rungs parallel (the ends of
the strands match) or antiparallel (the ends of the strands are opposites)?

one side ends in sugar the other ends


in phosphate
3. On the ladder model of DNA label each of the bases with the letter A, T, C or G.

A F T G G E C FE
4. Refer to Model 1. When one nucleotide contains adenine, what type of base is the adenine
attached to on the opposite nucleotide strand?

Thymine
5. The two strands of DNA are held together with hydrogen bonds between the nitrogen bases.
These are weak bonds between polar molecules. How many hydrogen bonds connect the two
bases from Question 4?

two FEET dashes


6. Refer to Model 1. When one nucleotide contains cytosine, what type of base is the cytosine
attached to on the opposite nucleotide strand?

guanine
7. How many hydrogen bonds connect the two bases from Question 6?
3 dashes
8. With your group, use three
DIE
a complete sentence to write a rule for how the bases are arranged in the
ladder model of DNA.
The adenine t thymine bases always pairtogether Guanine t cytosine also pair together
Read This!
Erwin Chargaff (1905–2002), an Austrian-American biochemist, investigated the ratio of nucleotide bases
found in the DNA from a variety of organisms. From his research, as well as research by Rosalind Frank-
lin and Maurice Wilkins, Watson and Crick developed the complementary base-pair rule during their
race to discover the structure of DNA. The complementary base-pair rule states that adenine and thymine
form pairs across two strands, and guanine and cytosine form pairs across two strands.
9. Fill in the complementary bases on the strand below according to the base-pair rule.
A T C C A G
T A G G T C
10. The ladder model of DNA is a simplified representation of the actual structure and shape of a
DNA molecule. In reality, the strands of DNA form a double helix. Refer to the double helix
diagram in Model 1 and describe its shape using a complete sentence.
A double helix is two twisted back bones made of alternating sugar t phosphate
The basepairs connect the backbones
in themiddle of the double helix
2 POGIL Activities for High School Biology

Model 2 – DNA Replication

Direction of DNA
helicase

Free Nucleotides

DNA helicase

11. Examine Model 2. Number the steps below in order to describe the replication of DNA in a cell.

4
______ Hydrogen bonds between nucleotides form.
______ Hydrogen bonds between nucleotides break.

d3
______ Strands of DNA separate.
______ Free nucleotides are attracted to exposed bases on the loose strands of DNA.
12. Locate the DNA helicase on Model 2.
a. What type of biological molecule is DNA helicase?

enzyme
b. What is the role of DNA helicase in the replication of DNA?

unzip the double helix


13. What rule is used to join the free nucleotides to the exposed bases of the DNA?

14. This type of replication is pairing


rule
base called semi-conservative replication. Considering the meaning of
these words (semi—half; conserve—to keep), explain why DNA replication is called
semi-conservative.

The new dna is built off of the


old half
DNA Structure and Replication 3
15. DNA molecules can be tens of thousands of base pairs in length. Mistakes in DNA replication
lead to mutations, which may or may not be harmful to an organism. How does semi-conservative
replication help prevent mutations during DNA replication?

The old DNA serves as a guide or template


so that the Dna is copiedexactly by base pairing
16. The proportions of the bases are consistent within a species; however they do vary between
species. Using the base-pair rules, complete the following table to show the percentage of each
type of base in the five different organisms.

Percentage of each type of base


Organism
Adenine Guanine Cytosine Thymine
Human 31 19 19
31
Cow 28 22
22 28
Salmon
2927 21
21 29
Wheat
23 23 27
Yeast 31 19
19 31

4 POGIL™ Activities for High School Biology


Extension Questions
Model 3 – Timing of DNA Replication
If the chromatin were
condensed, replicated
chromosomes would
be visible. (End of S)

S
Chromosomes are condensed.
G2 Matching sisgter chromatids are
connected by a centromere.
If the chromatin were (Beginning of M)
condensed, homologous
chromosomes would
be visible. (End of G1)
M

G1

17. According to Model 3, what term refers to loose DNA inside of a nucleus?

chromatin
18. During what part of the cell cycle is the DNA in a cell’s nucleus replicated?

s phase
19. During what part of the cell cycle is the DNA in a cell condensed into chromosomes?

m phase
20. Replicated chromosomes are often illustrated as an X shape to match how they look in real life
just before cell division.
a. According to Model 3, which of the following diagrams correctly show an original set of
homologous chromosomes (grey) and their sister chromatids (black)—the replicated portion?

0
b. What structure holds the two sister chromatids together as they prepare for cell division?

centromere
DNA Structure and Replication 5

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