INSTRUCTION MANUAL

Quick-DNA™ Plant/Seed Miniprep Kit

Catalog No. D6020

Highlights
• Simple method for the isolation of inhibitor-free, PCR-quality DNA (up to 25 µg/prep) from a variety
of plant and seed samples in as little as 20 minutes.

• The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays,
endonuclease digestion, etc.

• State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.

Contents
Product Contents & Specifications ....................... 1
Product Description….………………….……….. 2-3
Protocol………………………………….……………4
Supplementary MicroPrep Protocol ...................... 5
Ordering Information ............................................ 6

For Research Use Only Ver. 2.2.0

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
Page 1

Product Contents
Satisfaction of all Zymo
Research products is
Quick-DNA™ Plant/Seed Miniprep Kit D6020 Storage
guaranteed. If you should (Kit Size) (50 preps.) Temperature
be dissatisfied with this
product, please call ZR BashingBead™ Lysis Tubes (2.0 mm) 50 Room Temp.
1-888-882-9682.
BashingBead™ Buffer 40 ml Room Temp.
Genomic Lysis Buffer1 100 ml Room Temp.
DNA Pre-Wash Buffer2 15 ml Room Temp.
g-DNA Wash Buffer 50 ml Room Temp.
DNA Elution Buffer 10 ml Room Temp.
Prep Solution 30 ml Room Temp.
Zymo-Spin™ III-F Filters 50 Room Temp.
Zymo-Spin™ III-HRC Filters 50 Room Temp.
Zymo-Spin™ IICR Columns 50 Room Temp.
Collection Tubes 200 Room Temp.
Instruction Manual 1 -
Note - Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested
on a lot-to-lot basis to ensure they provide maximal performance and reliability.
1
For optimal performance, add beta-mercaptoethanol to 0.5%(v/v) i.e., 500 µl per 100 ml.
2
A precipitate may have formed in the DNA Pre-Wash Buffer during shipping. To completely resuspend the buffer,
incubate the bottle at 30 – 37 ºC for 30 minutes and mix by inversion. DO NOT MICROWAVE.

Specifications
• Format – Bead Beating, Spin Column.

• Sample Sources – Up to 150 mg that include leaves, stems, buds, flowers, fruit,
seeds, etc.
Note - ™ Trademarks of Zymo
Research Corporation. This
• DNA Yield – Typically 20-80 ng DNA/mg plant material.
product is for research use only
and should only be used by • DNA Purity – High quality, inhibitor-free DNA is eluted with DNA Elution Buffer and
trained professionals. It is not
intended for use in diagnostic is suitable for PCR amplification (A260/A280 > 1.8).
procedures. Some reagents
included with this kit are irritants.
Wear protective gloves and eye • DNA Size Limits – Capable of recovering genomic DNA up to and above 40 kb. In
protection. Follow the safety most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
guidelines and rules enacted by
your research institution or
facility. Disruptor Genie™ is a • DNA Recovery – Typically, up to 25 µg total DNA is eluted into 100 µl
trademark of Scientific
Industries, Inc. and FastPrep® is (50 µl minimum) DNA Elution Buffer per sample.
a registered trademark of
Qbiogene, Inc.
• Equipment – Microcentrifuge, vortex, cell disrupter/pulverizer (recommended).

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
 Page 2

Product Description

The Quick-DNA™ Plant/Seed Miniprep Kit is designed for the simple, rapid
isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources
including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can
be completed in as little as 15 minutes: plant samples (≤150 mg each) are added
directly to a ZR BashingBead™ Lysis Tube (2.0 mm) and rapidly and efficiently
lysed by bead beating without the use of organic denaturants or proteinases.
Polysaccharides and polyphenols/tannins are removed from the DNA using our Zymo-
Spin™ Technology, which includes the Zymo-Spin™ III-HRC filter, a PCR inhibitor
removal column. The eluted DNA is ideal for downstream molecular-based
applications including PCR, arrays, etc. A schematic of the Quick-DNA™ Plant/Seed
Miniprep Kit procedure is shown below.

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
Page 3

Milkweed Leaflet

Flowering Bud
Milkweed Leaf

Milkweed Pre-
A. thaliana

Juniper
Corn Sunflower
For Technical Assistance,
please contact those at M M Kernel Seed
Zymo Research’s
Technical Department at
1-888-882-9682 or E-mail to
[email protected].

Plant Seed
Comparison of DNA yields from various plant and seed samples using the Quick-DNA™
Plant/Seed Kit. Equivalent amounts of plant materials were processed with equal volumes of
eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size
marker (Zymo Research).

w/o w/
Zymo-Spin™ Zymo-Spin™
III-HRC III-HRC
M 0 0.1 0.01 0.001 0 0.1 0.01 0.001

PCR of diluted DNA (0 to 0.001) isolated with the Quick-

DNA™ Plant/Seed Kit from Arabidopsis thaliana leaf
samples demonstrates the effectiveness of the Zymo-
Spin™ III-HRC Column at removing PCR inhibitors from
the DNA. The arrow shows the relative migration of a
~700 bp amplicon from Chromosome 1 in a 0.8% (w/v)
agarose/ethidium bromide gel. M is a 100 bp DNA size
marker (Zymo Research Corp.)

M 0 0.1 0.01 0.001

PCR of diluted DNA (0 to 0.001) isolated with the Quick-

DNA™ Plant/Seed Kit from corn kernels. The arrow
shows the relative migration of a ~450 bp amplicon from
mitochondrial DNA in a 0.8% (w/v) agarose/ethidium
bromide gel. M is a 100 bp DNA size marker (Zymo
Research Corp.)

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
 Page 4

Protocol

For optimal performance, add beta-mercaptoethanol (user supplied) to the Genomic Lysis
Buffer to a final dilution of 0.5%(v/v) i.e., 250 µl per 50 ml or 500 µl per 100 ml.

1. Add up to 150 mg of finely cut plant or seed sample1 to a ZR BashingBead™ 1

Dried samples may be
Lysis Tube (2.0 mm). Add 750 µl BashingBead™ Buffer to the tube and cap hydrated in water prior to
processing to improve DNA
tightly. extraction efficiency.

2. Secure in a bead beater fitted with a 2 ml tube holder assembly and process at
maximum speed for ≥ 5 minutes.
Note: Processing time will vary based on sample input and bead beater. Times may be as little
as 5 minutes when using high-speed cell disrupters (FastPrep® -24) or as long as 20 minutes
when using lower speeds (e.g., Disruptor Genie).

3. Centrifuge the ZR BashingBead™ Lysis Tube (2.0 mm) in a microcentrifuge at

≥10,000 x g for 1 minute.

4. Transfer up to 400 µl supernatant to a Zymo-Spin™ III-F Filter in a Collection

Tube and centrifuge at 8,000 x g for 1 minute. Discard the Zymo-Spin™ III-F Filter.

5. Add 1,200 µl of Genomic Lysis Buffer to the filtrate in the Collection Tube from
Step 4. Mix well.

6. Transfer 800 µl of the mixture from Step 5 to a Zymo-Spin™ IICR Column2 in a 2

The Zymo-Spin™ IICR
Collection Tube and centrifuge at 10,000 x g for 1 minute. Column has a maximum
capacity of 800 µl.
7. Discard the flow through from the Collection Tube and repeat Step 6.

8. Add 200 µl DNA Pre-Wash Buffer to the Zymo-Spin™ IICR Column in a new
Collection Tube and centrifuge at 10,000 x g for 1 minute.

9. Add 500 µl g-DNA Wash Buffer to the Zymo-Spin™ IICR Column and centrifuge
at 10,000 x g for 1 minute.

10. Transfer the Zymo-Spin™ IICR Column to a clean 1.5 ml microcentrifuge tube and
add 100 µl (50 µl minimum) DNA Elution Buffer directly to the column matrix.
Centrifuge at 10,000 x g for 30 seconds to elute the DNA.

11. Place a Zymo-Spin™ III-HRC Filter in a clean Collection Tube and add 600 µl
Prep Solution. Centrifuge at 8,000 x g for 3 minutes.

12. Transfer the eluted DNA to a prepared Zymo-Spin™ III-HRC Spin Filter in a clean
1.5 ml microcentrifuge tube and centrifuge at exactly 16,000 x g for 3 minutes.

The filtered DNA is now suitable for PCR and other downstream applications.

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
Page 5

Ordering Information

Product Description Catalog No. Kit Size

Quick-DNA™ Plant/Seed DNA Kit D6020 50 preps.
Quick-DNA™ Plant/Seed DNA Kit D6021 2x96 preps.

For Individual Sale Catalog No. Amount

ZR BashingBead™ Lysis Tubes S6003-50 50

BashingBead™ Buffer D6001-3-40 40 ml

Genomic Lysis Buffer D3004-1-100 100 ml

DNA Pre-Wash Buffer D3004-5-15 15 ml

g-DNA Wash Buffer D3004-2-50 50 ml

DNA Elution Buffer D3004-4-10 10 ml

Prep Solution D6035-1-30 30 ml

Zymo-Spin™ III-F Filters C1057-50 50

Zymo-Spin™ IICR Columns C1078-50 50

OneStep™ PCR Inhibitor Removal Kit D6030 50

C1001-50 50
Collection Tubes C1001-500 500
C1001-1000 1,000

Lysis Instruments

Description Cat. No. Amount

Disruptor Genie™, 120V w/ 2 ml tube

S6001-2-120 1 unit
holder assembly.
Disruptor Genie™, 240V w/ 2 ml tube
S6001-2-240 1 unit
holder assembly.
TurboMix Attachment, 2 ml
Permanently mounts to most existing
S6004-2 1 unit
Vortex Genie™ mixers converting them
to a Disruptor Genie™.

The Disruptor Genie™ with 2 ml tube holder assembly from Scientific Industries, Inc.
(Cat. No. S6001-2-120 from Zymo Research Corp.)

ZYMO RESEARCH CORP.

Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com