2.8 Cell Respiration

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Topic 2.

8: CELL RESPIRATIOn
Cell Respiration ATP

Cell respiration is the controlled release of energy ATP (adenosine triphosphate) is a molecule that functions as an
from organic compounds to produce ATP immediate source of energy when hydrolysed (to form ADP)

The main organic compounds used are carbohydrates Pi


(i.e. glucose), but lipids or proteins may also be used +
ATP ADP
• Different organic compounds will have distinct
breakdown pathways and so have varied ATP yields High-energy bond Free energy

Glycolysis Anaerobic versus Aerobic Respiration

Cell respiration begins with the break down of glucose Pyruvate (from glycolysis) will follow one of two pathways:
via a process called glycolysis (occurs in the cytosol)
Anaerobic Respiration
• Glucose is broken down into pyruvate (×2)
• Occurs in the cytosol and does not require oxygen
• There is a small ATP yield (net gain = 2 ATP)
• Results in a small energy yield (2 ATP from glycolysis)
• Requires the reduction of NAD+ (to form NADH)
• Forms lactic acid (animals) or ethanol and CO2 (plants / yeast)
• Also known as fermentation and is reversible
2× ATP NAD+ ADP
Aerobic Respiration
• Occurs in the mitochondria and requires oxygen
Pyruvate • Results in a large energy yield (~36 ATP per glucose)
Glucose ADP NADH 4× ATP
(×2) • Forms carbon dioxide and water
INVEST PAYOFF • Uses hydrogen carriers to make ATP (oxidative phosphorylation)

Fermentation Respirometry

Fermentation is a reversible anaerobic process that allows A respirometer determines an organism’s respiration rate by
ATP production to continue in the absence of oxygen measuring either carbon dioxide production or oxygen uptake
• Commonly used for invertebrates or germinating seeds
Fermentation restores NAD+ stocks (needed in glycolysis)
to ensure a continued production of ATP (by glycolysis) A simple respirometer may involve the use of a manometer:
• An organism is sealed in a container with a CO2 absorbant
Fermentation in animals produces lactic acid, and is used
• Oxygen uptake creates a pressure change which displaces
to maximise muscle contractions when oxygen is limited
the fluid in the manometer (allowing for quantitation)
• This reaction can be reversed when oxygen is restored

Fermentation in plants and yeast produce ethanol and


CO2 gas which can be used in baking (leavening dough)
• Also for the production of alcohol, yogurts and cheese

GLYCOLYSIS FERMENTATION

glucose lactate ethanol + CO2


NAD+
ATP
NADH Platform A: Manometer Platform B:
Specimen and pressure change Equivalent
pyruvate CO2 absorbant moves the water volume / mass

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