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    AVIAVetHowTo02 TakeFTACardSamples EN15

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    Tanika dennie
    FTA cards are used to collect and transport biological samples for disease identification. They contain chemicals that lyse cells and stabilize DNA without risk of contamination. Samples can be taken via tissue impressions, swabs of organs or fluids, placed on the cards, and sent to a lab for PCR or genetic testing. Proper handling and labeling of FTA cards is required to ensure sample integrity. Common diseases and recommended sample types are listed.

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    © All Rights Reserved
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    AVIAVetHowTo02 TakeFTACardSamples EN15

    Uploaded by

    Tanika dennie
    33 views7 pages
    FTA cards are used to collect and transport biological samples for disease identification. They contain chemicals that lyse cells and stabilize DNA without risk of contamination. Samples can be taken via tissue impressions, swabs of organs or fluids, placed on the cards, and sent to a lab for PCR or genetic testing. Proper handling and labeling of FTA cards is required to ensure sample integrity. Common diseases and recommended sample types are listed.

    Original Description:

    Aviagen FTA Card Fact Sheet

    Original Title

    AVIAVetHowTo02-TakeFTACardSamples-EN15

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    FTA cards are used to collect and transport biological samples for disease identification. They contain chemicals that lyse cells and stabilize DNA without risk of contamination. Samples can be taken via tissue impressions, swabs of organs or fluids, placed on the cards, and sent to a lab for PCR or genetic testing. Proper handling and labeling of FTA cards is required to ensure sample integrity. Common diseases and recommended sample types are listed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    33 views7 pages

    AVIAVetHowTo02 TakeFTACardSamples EN15

    Uploaded by

    Tanika dennie
    FTA cards are used to collect and transport biological samples for disease identification. They contain chemicals that lyse cells and stabilize DNA without risk of contamination. Samples can be taken via tissue impressions, swabs of organs or fluids, placed on the cards, and sent to a lab for PCR or genetic testing. Proper handling and labeling of FTA cards is required to ensure sample integrity. Common diseases and recommended sample types are listed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 7

    How To...

    Take FTA Card Samples

    What is an FTA card?


    02
    • FTA (Flinders Technology Associates) cards are cotton-
    based, cellulose paper containing chemicals that burst cells,
    denature proteins and protect DNA, leaving a sample suitable for
    molecular identification without the risk of disease contamination.

    • They are a user-friendly way to send samples from the field to the
    laboratory for the identification of avian pathogens or biological analysis.

    What is an FTA card?

    01
    How to...
    Take FTA Card Samples

    02
    What are the uses of FTA cards?
    • FTA cards are used to confirm the presence of a disease/etiologic agent and allow the
    appropriate diagnosis and treatment.
    • Samples can be used for Polymerase Chain Reaction (PCR) testing, Real-time Polymerase Chain
    Reaction (RT-PCR) testing, and genetic sequencing allowing the rapid identification of suspected
    poultry diseases. Samples are in a format that is easy to use, store and ship to a laboratory.
    • There is no need for the use of phenol (a fixative used to preserve samples) or refrigeration,
    and there is no risk of contamination.

    Samples that can be taken with FTA cards


    • Blood
    • Cell culture
    • Allantoic fluid
    • Tissue impressions or scrapings
    • Tissue swabs
    • Bacterial culture solution
    What are the uses of FTA cards?

    02
    How to...
    Take FTA Card Samples

    Procedure 02

    Types of FTA cards


    • FTA cards are available in either white (classic) or pink (indicating) formats.
    • Although both classic and indicating cards can be used for sampling, it is recommended
    that classic FTA cards be used only for blood, as other samples may not show up very well
    on the white background. Indicating cards may be used for all types of samples as they
    contain a color-changing component that allows the user to see exactly where the sample
    was placed.
    • FTA cards come in a variety of configurations, allowing multiple options for the user.
    • Some of the most commonly used FTA cards have a 4 circle configuration.

    Examples of types and configurations of FTA cards.

    Examples of the most commonly used FTA cards, both classic and indicating.

    Care and handling of FTA cards


    • Unused FTA cards should be stored at room temperature (20-24°C, 68-75°F) in a zip-
    sealed (zip-lock) plastic bag if possible. Cards should be protected from light to ensure that
    the chemicals contained within are not damaged.
    • Always wear gloves when handling FTA cards to ensure that contaminants from human
    hands are not transferred onto the card.
    Procedure

    03
    How to...
    Take FTA Card Samples

    Important notes when taking FTA samples 02


    • Use fresh tissues for sampling and avoid using dead birds found in the pen when
    possible, as nucleic acid quality could be compromised.
    • Use ethanol to disinfect handling equipment between samples.
    • Clearly label the card/and or application circles with the identity of the sample.
    • Use only a pencil to write on FTA cards as ink from a pen may run, possibly contaminating
    the sample.

    Equipment needed for sampling


    • Scissors
    • Scalpel
    • Forceps
    • Gloves
    • Sterile swabs in individual containers
    • FTA Card
    • Pencil for identifying sample
    • Syringe for liquid samples
    Procedure

    04
    How to...
    Take FTA Card Samples
    Suspected diseases and recommended
    samples to confirm diagnosis
    02
    Suspected Disease Recommended Samples
    Tracheal scraping / impressions
    Kidney impressions and tracheal
    Infectious Bronchitis (IB) scraping (renal)
    Cecal tonsils impressions
    Allantoic fluid
    Tracheal and eyelid scraping /
    Infectious Laringotracheitis (ILT) impressions
    Lung impressions
    Tracheal scraping and cecal tonsils
    Newcastle Disease (ND)
    impressions
    Infectious Bursal Disease (IBD) Bursal impressions
    Reovirus (Reo) Synovial fluid, liver and heart impressions
    Malabsorption Syndrome / Runting Gut scraping of duodenum, ileum and /
    Stunting Syndrome (RSS) or jejunum
    Avian Pneumovirus / Turkey
    Rhinotracheitis (TRT) / Swollen Head Turbinate impressions / scraping (acute)
    Syndrome (SHS)
    Inclusion Body Hepatitis
    Liver impressions from affected birds
    Avian Adenovirus
    Gut scraping of the duodenum, ileum and
    Enteric Viruses
    / or jejunum
    Thymus impressions, bone marrow
    Chicken Anemia Virus
    impressions
    Apply culture with loop or swabs or
    culture can be diluted to 0.5 McFarland
    Salmonella
    before placing 50 microliters or two
    drops of the solution in the card
    Mycoplasma Tracheal swab, impressions or scraping
    Marek’s Disease Virus (MDV) Tissue impressions / feather pulp sample
    Reticuloendotheliosis Virus (REV) Tissue impressions
    Avian Leukosis Virus (ALV) Tissue impressions
    Procedure

    05
    How to...
    Take FTA Card Samples

    Procedure for taking tissue impressions 02


    Step 1 Cut the organ open so that either the mucosa or the
    internal tissue is exposed.
    Step 2 Using a scalpel and forceps, cut a sample of tissue
    and make a generous impression on the FTA card
    Step 1
    by pressing and rubbing the cut tissue onto the
    application circle.
    Step 3 Remove the cut tissue from the card, leaving behind
    the impression.
    Step 4 Let the impression air dry on the card for at least 30
    Step 2-3
    minutes, keeping the card away from extreme heat,
    humidity or direct sunlight.

    Procedure for taking swab impressions


    Step 1 Cut the internal organ open so that either the mucosa
    or internal tissue is exposed.
    Step 2 Thoroughly swab the sampling area with a sterile
    swab.
    Step 2
    Step 3 Transfer the contents of the swab onto the FTA card
    by pressing it onto the application circle. It may be
    best to use an indicating FTA card to ensure that
    Step 2
    the sample can be identified within the circle.
    Step 4 Let the impression air dry on the card for at least 30 minutes, keeping the card
    away from extreme heat, humidity or direct sunlight.
    Note - Swab samples from live bird orifices can be used for FTA, however these samples
    should be collected humanely and by a trained individual.

    Procedure for taking liquid impressions


    Step 1 Using a graduated pipet or syringe, remove 5-50
    microliters (or two drops) of liquid (whole blood,
    allantoic fluid, etc.) from the sampling area.
    Step 2 Place the sample within the application circle on the
    Step 1
    FTA card.
    Step 3 Let the impression air dry on the card for at least
    30 minutes, keeping the card away from extreme
    heat, humidity or direct sunlight.
    Note - Blood samples from live bird veins may be used for FTA, Step 2
    however these samples should be collected humanely and by
    a trained individual.

    Correct storage of FTA cards


    • After the FTA card is dry, put it into an air-tight, zip-sealed (zip-loc) plastic bag, ensuring
    the card is kept as dry as possible.
    Procedure

    • The FTA card is now ready to be sent to a laboratory that is properly equipped to run the
    desired analyses.
    06
    How to...
    Take FTA Card Samples

    Sending FTA cards to the laboratory 02


    • Due to the biological hazard with sending tissue samples through the mail,
    you must obtain the proper documentation from the receiving laboratory before
    sending samples.
    • When sending FTA cards, you must have the following:
    o An updated import permit (should be sent from the receiving laboratory in the case of
    the U.S.A).
    o Correctly filled-in laboratory submission form.
    o Proper customs declaration form (for international shipments) from the relevant
    authorities.

    Sending FTA cards to the laboratory

    07

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