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Lab Report 3 - 2023

This document outlines a lab report assignment on polymerase chain reaction (PCR) and Western blot techniques. Students must investigate the theoretical concepts and practical applications of PCR and Western blot for evaluating DNA and protein damage caused by exposure to a selected pollutant. The report should include sections on introduction, applications of the techniques, references, and organization. The introduction covers theoretical concepts and required instrumentation/reagents for PCR, electrophoresis, and Western blot. The applications section addresses general uses/limitations and how the techniques can study cell alterations from pollutant exposure. References must be in APA style from reliable sources.

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María Fernanda Ruiz Guerra
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38 views2 pages

Lab Report 3 - 2023

This document outlines a lab report assignment on polymerase chain reaction (PCR) and Western blot techniques. Students must investigate the theoretical concepts and practical applications of PCR and Western blot for evaluating DNA and protein damage caused by exposure to a selected pollutant. The report should include sections on introduction, applications of the techniques, references, and organization. The introduction covers theoretical concepts and required instrumentation/reagents for PCR, electrophoresis, and Western blot. The applications section addresses general uses/limitations and how the techniques can study cell alterations from pollutant exposure. References must be in APA style from reliable sources.

Uploaded by

María Fernanda Ruiz Guerra
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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SD1003B Cellular and Molecular Mechanisms

Lab Report
Practice 3. Polymerase Chain Reaction (PCR) and Western Blot

Challenge:
How can you make society aware of the effect that exposure to diverse pollutants
has on their health and life quality?

Aim:
To know the theoretical fundaments and the practical application of PCR and
Western Blot for the evaluation of DNA & protein damages caused by the exposure
to the selected pollutant for the proposed challenge.

1. General Information
1.1. Date of the practice: 24/08/2023
1.2. Information about the team
Group: 3
Team number:
Selected pollutant:

1.3. Team members:

Full Name Student ID (A0******)


SD1003B Cellular and Molecular Mechanisms

1.4. Name of the Professor: Dra. Mirna A. González González


2. Introduction (one page maximum)
2.1. Theoretical concepts of PCR, electrophoresis & Western Blot……...……………...10
pts.
2.2. Instrumentation and reagents required for the performance of PCR, electrophoresis
and Western Blot…………………………………………………………………….……15
pts.

3. Applications of the technique (one page maximum)


3.1. General applications and drawbacks of PCR & Western Blot……………………....25
pts.
3.2. Application of PCR & Western Blot for the study of specific cell alterations caused by
the exposure of the selected pollutant……………………………………………..25
pts.
3.3. Critical self-reflection about the ethical implications that PCR & Western Blot may have
in research and/or its benefit for society……………………………………………..5
pts.

4. References (APA style)………...……………………………...……………………….10


pts.
4.1. There are different online resources to automate the process of generating the
references in APA style. Example: https://www.scribbr.es/detector-de-plagio/generador-apa/
4.2. At least 5 reliable sources should be consulted. Scientific journals, institutional
websites, books, thesis, academic databases are considered reliable sources. Do
not use information from commercial websites (or justify their use), divulgation
websites such as Wikipedia, as well as websites that promote bad academic
practices such as “Buenas Tareas” and “Rincón del Vago”.

5. Organization, writing and spelling………..………………….…………….10 pts.

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