Sartorius Liquid Handling Products

2 Table of Contents

Table of Contents
⹅⹅

⹅⹅
About Sartorius Liquid Handling ⹅⹅
Pipette Tips
  5 About Us 54 Pipette Tips
  7 Responsible Manufacturing 56 Packaging Options
8 Outstanding Product Quality 58 Optifit Tips
10 Ergonomics, Design and Reliability 60 SafetySpace Filter Tips
61 Low Retention Tips

⹅⹅
Pipette Selection Guide 62 Ordering Information
12 Electronic or Mechanical Pipette
⹅⹅
Maxi-volume Liquid Handling
68 Midi Plus Pipetting Controller
⹅⹅
Electronic Pipettes
70 Proline® Prospenser Bottle-top Dispenser
17 Picus® and Picus® NxT
71 Prospenser Bottle-top Dispenser
22 eLINE®
72 Biotrate Digital Burettes
25 Pipette Tip Selection Guide
73 Ordering Information and
Performance Specifications
⹅⹅
Mechanical Pipettes
28 mLINE®
32 Proline® Plus ⹅⹅
Pipetting Academy
76 Pipetting Academy
38 Proline®
78 Pipetting Recommendations
42 Pipette Tip Selection Guide

⹅⹅
Stands & Accessories ⹅⹅
Calibration and Maintenance Services
46 Pipette Stands 82 Pipette Calibration and Maintenance Services

47 Elbow Pad 88 Pipette Decontamination Procedure

48 Safe-Cone Filters 89 Autoclaving Instructions

50 Reagent Vessel 90 Troubleshooting Guide

50 Calibration Tool|Tube Opener &

Colour-coding Caps
3
4 About Sartorius Liquid Handling About Us

Kajaani, Finland
Helsinki, Finland
St. Petersburg, Russia

Nieuwegein, Netherlands
Taastrup, Denmark
Vilvoorde, Belgium
Aachen, Germany
Dublin, Ireland Hamburg, Germany
Stonehouse, UK Goettingen, Germany
Epsom, UK Poznan, Poland
Paris, France Guxhagen, Germany
Aubagne, France Moscow, Russia
Lourdes, France Budapest, Hungary
Madrid, Spain
Vienna, Austria
Mississauga, Ontario, Canada
Tagelswangen, Switzerland Beijing, China
Denver, CO, USA Bohemia, NY, USA
Florence, Italy Seoul, South Korea
Milan, Italy Tokyo, Japan
Suzhou, China Shanghai, China
Hong Kong, China
Mexico City, Mexico Hanoi, Vietnam
Yauco, Puerto Rico Mohamdia, Tunisia
Bangkok, Thailand
Bangalore, India Makati City, Philippines

Kuala Lumpur, Malaysia

Singapore, Singapore

São Paulo, Brazil

Buenos Aires, Argentina

Melbourne, Australia

Sales
Production
 About Us About Sartorius Liquid Handling 5

About Us
⹅⹅

Sartorius Liquid Handling business unit is a leading, global provider of

electronic and mechanical pipettes, disposable pipette tips and related
services, for pharmaceutical and chemical industry, clinical laboratories,
research institutes and universities.

Known as the forerunner in developing ergonomic pipettes with user comfort

and health in mind, Sartorius is a trusted supplier to laboratory professionals,
who want to reduce their risk of work-related injuries. Strong technical
innovations and the use of the newest technologies both in design and
manufacturing ensure the highest reliability and quality of Sartorius’ liquid
handling products. Sartorius offers pipette maintenance, repair and calibration
services globally through its certified service centres.

Sartorius’ liquid handling competence center is based in Helsinki, Finland,

while the products are manufactured both in Finland and in China. The sales
network of Sartorius is global, covering 110 countries.

Sartorius is one of the world’s leading providers of laboratory and process

technologies and equipment, covering the segments of Bioprocess Solutions
and Lab Products & Services. Founded in 1870, the company, based in
Göttingen, Germany, currently employs more than 5 500 people around the
world. Sartorius has its own production facilities in Europe, Asia and America.
6 About Sartorius Liquid Handling Responsible Manufacturing

Responsible Manufacturing
⹅⹅

We pay particular attention to the environmental impact of our operations.

We aim to minimize the environmental load of our products throughout their
lifecycle.

Environmentally Friendly Design and Manufacturing

Sartorius complies with ISO 14001 environmental standards. Already at the
design stage, we look into ways of reducing the usage of hazardous substances
and materials. Our products are also designed to minimise waste during
manufacturing and use. For example, the tip refill system reduces waste up to
61% compared to racked tips, and the FlexiBulk tip pack up to 50%, compared
to traditional bulk tip packages. We have invested in production technologies
that generate less waste. We also use environmentally friendly carbon-free
electricity at our production facility in Kajaani, Finland.

Recyclable Materials
The plastic materials used for the products and their packaging are suitable
for use in waste-to-energy facilities. For example, the tips and the tip racks
made of 100% polypropylene (PP) can be fully recycled as energy waste – PP
could also be reused. The cardboard packages can be recycled (reused).
 Responsible Manufacturing About Sartorius Liquid Handling 7

⹅⹅
In Practice
‒‒ Package materials are suitable for recycling either as materials or as energy
waste

‒‒ Package sizes are minimised in order to use less material and to ensure
efficient logistics

‒‒ Pipette tips and racks are 100% recyclable as energy waste or as material

‒‒ Cadmium-free plastics have been used since 1994

‒‒ Cadmium-free batteries have been used since 1996

‒‒ mLINE® and Proline® Plus mechanical pipettes are more than 90% recyclable
as energy waste

‒‒ Electronic pipettes are WEEE/RoHS compliant, which means controlled

recycling by national authorities

‒‒ Environmentally friendly carbon-free electricity is used at our production

facility in Kajaani, Finland

‒‒ Minimum possible amount of paper and energy is used in our offices

‒‒ Environmentally friendly paper is used for printing

8 About Sartorius Liquid Handling Outstanding Product Quality

Outstanding Product Quality

⹅⹅

Sartorius’ products are developed and manufactured according to the

requirements of the ISO 9001, ISO 13485 and ISO 14001 quality and
environmental standards. Tip production also follows the ISO 14644-1
standard, in order to fulfil ISO class 8 cleanroom conditions. Our accredited
pipette calibration follows the ISO 17025 standard. Our pipettes are supplied
with individual quality control certificates.

We continuously develop our products and processes in order to meet, and

often exceed, the demands of regulatory authorities, environmental bodies,
and most importantly, our customers.

Sartorius’ products are developed and manufactured according to the

requirements of the ISO 9001, ISO 13485 and ISO 14001 quality and
environmental standards. Tip production also abides by the ISO 14644-1
standard, in order to fulfil ISO Class 8 Cleanroom conditions. ISO 13485 is a
specific standard for medical device quality systems, and supplementing the
more generic ISO 9001 standard, which applies to many industries.

The Finnish national accreditation body operates independently as part of

the Measurement Technology Centre (MIKES). Accredited pipette calibration
laboratories in Finland, Germany, France, UK, China and Japan calibrate
pipettes according to precise technical requirements. Our calibration
laboratories in Finland, Germany, France, UK, China and Japan have been
granted this status by their national accreditation bodies.

Sartorius offers a 2-year warranty for all mechanical and electronic pipettes.
The low lifetime cost and environmental friendliness of our products, which
have long warranty periods, give a high return on investment.

We follow these manufacturing quality standards

⹅⹅
ISO 9001 ∙ ISO 13485 ∙ ISO 14001 ∙ ISO 17025
Outstanding Product Quality About Sartorius Liquid Handling 9

The ergonomic design label indicates products, which Sartorius has designed
specifically to reduce the risk of work-related hand, arm and shoulder
disorders, such as RSI (Repetitive Strain Injury).

The Optiload tip loading mechanism developed by Sartorius in mLINE®,

Proline® Plus, eLINE®, Picus® and Picus® NxT pipettes allows tips to be loaded
with constant force. This secures optimal tip sealing and minimum tip
ejection force.

Every lot of Sartorius Single Tray, Refill Pack and FlexiBulk tips are certified
to be free of DNase, RNase and endotoxins, for the protection of samples
from contamination. This certificate can be downloaded from www.sartorius.
com, in the Liquid Handling section. Sartorius’ tip production is ISO 8
cleanroom classified, which ensures a contamination-free manufacturing
environment, and products.

Most Sartorius liquid handling products are autoclavable. Please see details in
the following product specific chapters..
10 About Sartorius Liquid Handling Ergonomics, Reliability and Design

⹅⹅ Ergonomics, Design and Reliability

Three key factors – ergonomics, design and reliability – form the cornerstone
of our product development. The newest family member, the Picus® electronic
pipette, is an excellent example of combining all of these aspects in one
product. It is the lightest and smallest electronic pipette on the market
reducing the risk of strain injury. Its high reliability and ease of use make it a
valued instrument for professionals, who strive for high quality results.
Thanks to its functional and well-rounded design suitable for laboratory
setting, it has also received multiple design awards. All Sartorius Liquid
Handling products are designed in Finland, where our R&D team is constantly
seeking solutions to further improve liquid handling instruments to make lab
life easier.

“
Designing products that people work with on a daily basis is always
challenging. Many users are interviewed and multiple aspects need to be
taken into account, to combine excellent ergonomics and easy usability with
today’s technology and features. To solve this puzzle and come up with a
great product is an exciting, but sometimes tough, journey. However, it is
always rewarding in the end.”

– Ville Hintikka, Chief Designer at Sartorius

⹅⹅
Ergonomics
When designing a pipette, we always consider the shape and function of the
human hand. As we understand the risks of repetitive pipetting, we emphasise
ergonomic design in every product we make. Simply put, this means products
that you can use in a comfortable posture with minimum muscle power.
Our pipettes and dispensers are designed for both right- and left-handed
users. Their operating buttons are located sufficiently close together, within
ergonomic reach of the thumb.

⹅⹅
Design
We provide products with a timeless and light, yet practical, design, suitable
for laboratory settings and pleasing to the eye of the user. The newest
member of our product family, the Picus® electronic pipette, won the Red Dot
design award and the Fennia Prize Honorary Mention in 2012, as well as the
German Design Award in 2014. Our other electronic pipette family, eLINE®,
was given an honorary mention in the Pro Finnish Design competition at the
time of its launch.
 Ergonomics, Reliability and Design About Sartorius Liquid Handling 11

⹅⹅
Reliability
For us, reliability has many aspects, the most important being
the accuracy and precision of the results and the secured
purity.

The core of a pipette lies in its accuracy and precision. For this
reason, we have used the newest technologies together with
in-house innovations, to achieve even more reliable pipetting
results. Our electronic brake, piston control system and plate
tracker for electronic pipettes are our latest innovations.
They increase accuracy, precision and reliability of the device.
Another important factor in achieving reliable results is the
optimal tip fit, which we can guarantee by designing and
producing the tips ourselves, to perfectly match our pipettes.

Because purity is the key concern in many laboratories, we

offer special Safe-Cone Filters for our pipettes, to prevent
them from being contaminated by aspirated samples. We
strive to produce as many autoclavable products as possible,
both pipettes and tips. Our pipette tips are manufactured in
ISO Class 8 Cleanroom conditions. We test every certified tip
lot for DNase, RNase and endotoxins at an external laboratory.
We also offer an innovative SafetySpace filter tip range for
safer and contamination-free pipetting.
12 Pipette Selection Guide Electronic or Mechanical Pipette

Pipette Selection Guide

⹅⹅

⹅⹅
Electronic or Mechanical Pipette

Are you looking for a pipette for sterile work, or one you could easily calibrate
yourself? Or are you seeking for a really light and ergonomic solution?
Perhaps you need a pipette with a certain pipetting mode to speed up your
work? By consulting the tables below, you can choose the instrument that
best suits your needs.

Electronic or Mechanical Pipette

Features Electronic Pipettes Mechanical Pipettes

Highest ergonomics 
Fastest pipetting 
User-independent results 
Multiple pipetting modes 
Fully autoclavable 
Calibration by user 1
 

1) Picus® and Picus® NxT only

Mechanical Pipettes

Features mLINE® Proline® Plus Proline®

Most ergonomic 
1
Pipetting forces 12 N 15 N 20 N
Fully autoclavable  
Optiload tip loading all models multi-channels
mechanism only
Weight1 77 g 82 g 84 g
Safe-Cone Filters   
Filter ejector 
Volume locking  click stops click stops
Colour-coding on pipette  
Colour-coding caps 
ID tags 
Warranty for 2 years   

1) 1000 µl 1-channel models

 Electronic or Mechanical Pipette Pipette Selection Guide 13

Electronic Pipettes

Features Picus® NxT Picus® eLINE®

Most ergonomic  
1
Weight 100 g 100 g 170 g
Length1 210 mm 210 mm
Volume range, single-channels 0.2 µl -10 ml 0.2 µl -10 ml 0.1 µl - 5 ml
Volume range, multi-channels 0.2 µl -1.2 ml 0.2 µl -1.2 ml 0.2 µl -1.2 ml
Language options2 5 5 1
Pipetting modes 8 8 7
Advanced functions 7 6 3
Repeated blow-out (advanced function) 
Microwell plate tracker  
Memory places (for storing programs) 10 10 6
Information on service & calibration intervals  
Password protection 
Certificate of accredited 3-point calibration 
Electronic tip ejection   
Calibration adjustment by user  
Hot key for stored programs  
Safe-Cone Filters   
Autoclavable lower parts   
Optiload in multi-channels   
Colour-coding on pipette   
Warranty for 2 years   

1) 300 µl 1-channel models

2) English, French, German, Chinese and Russian
 Table of Contents Electronic Pipettes 15

Electronic Pipettes
⹅⹅
Table of Contents

17 Picus® and Picus® NxT

22 eLINE®
25 Pipette Tip Selection Guide
 Picus® & Picus® NxT Electronic Pipettes 17

Picus® and Picus® NxT Electronic Pipettes

⹅⹅
The Most Sophisticated and Ergonomic Pipettes Ever!

The award-winning Picus® is designed the unique plate tracker increases

to revolutionize pipetting. Being reliability in microwell plate
the smallest and lightest electronic dispensing, by guiding the steps to
pipette on the market, it eases pipette into the correct microplate
the user’s workload and provides wells.
protection from repetitive strain
injury (RSI). Its new generation Safe-Cone Filters, inserted in
technology, electronic brake and the tip cone, reduce the risk of
piston control system guarantee contamination cost-effectively.
accurate and precise pipetting
results. By guiding pipetting steps, Fast Execution Of Pipetting Tasks
the unique plate tracker increases The unique adjustment wheel of
reliability in microwell plate work. Picus® pipettes offers extremely fast
volume setting and menu navigation.
These same qualities can also be The user can perform pipetting
found in Picus® Nxt with added tasks quickly and easily with the
features for enhanced reliability in extensive range of pipetting modes,
strictly regulated laboratories. from diluting and titrating to serial
dispensing.
Picus® and Picus® NxT are available
in single-channel models, covering a The pipetting functions are quick
volume range of 0.2-10 000 μl and in to learn, using the intuitive user
multi-channel models from 0.2 μl to interface, available in a choice of
1200 μl. language options: English, French,
German, Russian and Chinese.
Superior Ergonomics
The uniquely lightweight and Picus® NxT for Highly Regulated
streamlined design of the Picus® Laboratories
pipettes ensures effortless pipetting The following features are only
experience. The comfortable handle available with Picus® NxT pipettes,
and the finger hook enable the which are especially intended to
pipette to rest lightly in the user’s conform to the strictest laboratory
hand, with minimum effort. regulations and requirements:

The conveniently located soft-touch ‒‒ Certificate of accredited 3-point

operating button and electronic tip calibration (per ISO 17025 and
ejection help minimize muscle strain, ISO 8655)
further enhancing the ergonomics of ‒‒ Password functionality protects the
the pipette. stored programs from unauthorized
editing
Reliable Results ‒‒ Repeated blow-out function helps
The electronic brake and piston to dispense the very last droplets
control system guarantee accurate of liquid, ensuring complete sample
and precise pipetting results, recovery
independent of the user. Using
18 Electronic Pipettes Picus® & Picus® NxT

⹅⹅
Features and Benefits
Ergonomic Design for Reduced Risk
of Strain Injuries
‒‒ Extremely compact and light
(down to only 100 g) design
maximizes user comfort
‒‒ Conveniently located, soft-touch
operating button and electronic
tip ejection help minimize muscle
strain and reduce the risk of
repetitive strain injuries (RSI)
‒‒ Comfortable handle design and
finger hook allow the pipette to
rest effortlessly in hand

Innovative Technology for

Reliable Results
‒‒ Enhanced DC-motor concept
provides outstanding accuracy and
repeatability of results
‒‒ Electronic brake stops piston
movement rapidly and precisely,
ensuring high precision, especially
in serial dispensing
‒‒ Optical sensor controls and
monitors piston movement in real
time, ensuring unbeatable accuracy
and reliability
‒‒ With Picus® NxT only: Repeated
blow-out function helps dispense
the very last droplets of liquid,
ensuring complete recovery

Intuitive User-Interface for Ease

of Use
‒‒ Available with five language
options: English, Chinese, French,
German and Russian

Comprehensive Range of Pipetting

Modes for Speed of Use
‒‒ The 8 main modes combined with
Advanced functions enable fast
and handy execution of various
pipetting tasks

Autoclavable
lower parts
 Picus® & Picus® NxT Electronic Pipettes 19

Handy Adjustment Wheel for Fast

and Effortless Operation
‒‒ Offers extremely fast volume
setting and easy menu navigation
‒‒ Enables ergonomic one-handed
operation
‒‒ Controls accurately, manual
pipetting and titrating speed, with
just a light touch of the thumb

Microwell Plate Tracker for

Fast charging, here with Charging Adjustment wheel for quick Improved Efficiency and Reliability
Carousel, for example single-handed operation and ‒‒ Unique built-in tracker for 96 and
speed control
384 well plates guides the user to
pipette into the correct wells
‒‒ Improves work efficiency and
reliability of results

Optiload for Perfect Tip Sealing

‒‒ Allows tip loading with an equal
constant force onto every channel
‒‒ Enables perfect tip sealing onto
every individual tip cone
‒‒ Is available in multi-channel
models

Safe-Cone Filters for insertion Optiload for a tight fit and equal Safe-Cone Filters to Prevent
into every channel’s tip cone, to sealing on every channel’s tip Contamination
prevent contamination of pipette cone ‒‒ Are available in all models > 10 μl

Autoclavable Lower Parts for Easy

Sterilisation
‒‒ Enable easy cleaning for reduced
risk of contamination (excl.
1200 μl models)

Calibration Adjustment
‒‒ Enables better accuracy of the
results e.g. with non-aqueous
liquids (viscous, volatile)
‒‒ The adjustment can be made in
Calibration Adjustment in use as
indicated by ADJ1
1, 2 or 3 points by the user.

Fast Charging
‒‒ Li-Polymer battery enables
charging time of approx. 1 hour

Microwell plate tracker in use

20 Electronic Pipettes Picus® & Picus® NxT

⹅⹅
Pipetting Modes
Picus® & Picus® NxT

Pipetting Modes Advanced Functions*

Tracker Mixing Counter Excess Volume Auto- Repeated Fast
Adjustment dispensing Blow-out** Dispensing
Pipetting     
Reverse Pipetting    
Multi-dispensing    
Manual Pipetting  
Diluting   
Sequential Dispensing  
Multi-aspiration  
Titrate  

* Advanced functions are used in conjunction with the pipetting mode.

** Only available in Picus® NxT models
 Picus® & Picus® NxT Electronic Pipettes 21

⹅⹅
Ordering Information
Picus® & Picus® NxT

Picus® Picus® NxT Channels Volume Colour- Increment Test Systematic Random Safe-Cone Filters
Range (µl) Coding (µl) Volume (µl) Error* (%) Error* (%) Standard Plus
735021 LH-745021 1 0,2-10  0,01 10 0,90 0,40 – –
5 1,00 0,70
1 2,50 1,50
735041 LH-745041 1 5-120  0,10 120 0,40 0,15 721008 721018
60 0,60 0,20
12 2,00 1,00
735061 LH-745061 1 10-300  0,20 300 0,40 0,15 721007 721017
150 0,60 0,20
30 1,50 0,80
735081 LH-745081 1 50-1000  1,00 1000 0,40 0,15 721006 721016
500 0,60 0,20
100 1,50 0,50
735101 LH-745101 1 100-5000  5,00 5000 0,50 0,15 721005 721015
2500 0,80 0,20
500 1,00 0,40
735111 LH-745111 1 500-10000  10,00 10000 0,60 0,20 721005 721015
5000 1,20 0,30
1000 3,00 0,60
735321 LH-745321 8 0,2-10  0,01 10 0,90 0,50 – –
5 1,50 0,80
1 4,00 3,00
735341 LH-745341 8 5-120  0,10 120 0,50 0,20 721008 721018
60 0,70 0,30
12 2,00 1,50
735361 LH-745361 8 10-300  0,20 300 0,50 0,20 721007 721017
150 0,70 0,30
30 2,00 1,00
735391 LH-745391 8 50-1200  1,00 1200 0,50 0,20 721006 721016
600 1,00 0,30
120 2,50 1,00
735421 LH-745421 12 0,2-10  0,01 10 0,90 0,50 – –
5 1,50 0,80
1 4,00 3,00
735441 LH-745441 12 5-120  0,10 120 0,50 0,20 721008 721018
60 0,70 0,30
12 2,00 1,50
735461 LH-745461 12 10-300  0,20 300 0,50 0,20 721007 721017
150 0,70 0,30
30 2,00 1,00
735491 LH-745491 12 50-1200  1,00 1200 0,50 0,20 721006 721016
600 1,00 0,30
120 2,50 1,00

* The listed systematic and random error values are valid for Pipetting (P) mode only and are achieved under strictly controlled conditions during
type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error values may change
without prior notice.
All pipettes are supplied with a universal charger (EU, UK, US | JPN, KOR, AUS and CHN plugs)
22 Electronic Pipettes eLINE®

eLINE® Electronic Pipettes

⹅⹅
Proven Convenience, Reliability and Speed

The electronic eLINE® pipette family the easy-to-reach operating buttons,

offers a great price-performance allow for convenient single-handed
ratio for any user looking for a cost- operation for both right and left-
effective companion for repetitive handed users. The convenient finger
pipetting, where convenience, hook provides proper support to
accuracy and speed are mission- minimize grip force.
critical.
Reliable Results
eLINE®’s ergonomic design eases the Electronic piston control guarantees
user’s workload. The fully electronic accurate and reliable pipetting
operation with electronic piston results, independent of the user. The
control secures accurate and precise Optiload feature in multi-channel
Optiload for a tight fit and equal
sealing on every channel’s tip results independent of the user. models permits tip loading with
cone. perfect tip sealing onto every tip
eLINE® is available as single-channel cone for secured accuracy. Safe-
models, covering a volume range of Cone Filters prevent the risk of
0.1 to 5000 μl, and as multi-channel contamination cost-effectively.
No rocking needed
models from 0.2 to 1200 μl.
Speed
Convenience The versatile pipetting modes
Fully electronic operation minimizes reduce the work stages needed,
the force needed to operate the enabling significantly faster liquid
pipette and the electronic tip ejection handling than a mechanical pipette.
ensures light removal of tips also Electronic multi-channel models
with multi-channel models. The enable pipetting on microwell plates
ergonomic design of the handle, and considerably faster than mechanical
With Optiload, no rocking is
needed when attaching tips.
ones.
⹅⹅
Features and Benefits
Ergonomic Design for Reduced Risk Wide Selection of Pipetting Modes
of Strain Injuries for Speed of Use
‒‒ Conveniently located, soft-touch ‒‒ The 7 pipetting modes enable fast
operating button and electronic and handy execution of various
tip ejection help minimize muscle pipetting tasks
strain and reduce the risk of
repetitive strain injuries (RSI) Optiload for Perfect Tip Sealing
‒‒ Comfortable handle design and ‒‒ Allows tip loading with an equal
finger hook allow the pipette to constant force onto every channel
rest effortlessly in hand ‒‒ Enables perfect tip sealing onto
The increased liquid dispensing every individual tip cone
speed of the eLINE® 0.1-5µl pipette Innovative Technology for
with Super Pipetting feature ‒‒ Is available in multi-channel
enables liquid dispensing in the Reliable Results models
air, emptying the tip completely, ‒‒ Enhanced DC-motor concept
without a droplet remaining. provides outstanding accuracy and Safe-Cone Filters to Prevent
repeatability of the results Contamination
‒‒ Electronic brake stops piston ‒‒ Are available in all models > 10 μl
movement rapidly and precisely,
ensuring high precision, especially Autoclavable Lower Parts for Easy
in serial dispensing Sterlisation
‒‒ Optical sensor controls and ‒‒ Enable easy cleaning for reduced
monitors piston movement in real risk of contamination
time, ensuring unbeatable accuracy (excl. 1200 μl models)
and reliability
 eLINE® Electronic Pipettes 23

Autoclavable
lower parts

⹅⹅
Pipetting Modes
Main Mode Advanced Functions
Mixing Counter Auto-dispensing (Timed)
Pipetting   
Reverse Pipetting 
1
Manual Pipetting 
Multi-dispensing  
Diluting  
Sequential Dispensing2 
Multi-aspirating 
Super Pipetting  Only available in eLINE® 0,1-5 µl

1) Not available in eLINE® multi-channel pipettes

2) Not available in eLINE® 0,1-5 µl
24 Electronic Pipettes eLINE®

⹅⹅
Ordering Information
eLINE®

Order Channels Volume Colour- Increment Test Systematic Random Safe-Cone Filters
Code Range (µl) Coding (µl) Volume (µl) Error* (%) Error* (%) Standard Plus
730011 1 0,1-5  0,05 5 1,20 0,70 - -
2,5 2,00 1,20
0,5 12,00 8,00
730021 1 0,2-10  0,05 10 1,00 0,40 - -
5 1,40 0,70
1 4,00 2,30
730041 1 5-120  0,50 120 0,60 0,20 721008 721018
60 0,90 0,20
12 3,00 1,00
730061 1 10-300  1,00 300 0,60 0,15 721007 721017
150 0,60 0,20
30 1,50 0,80
730081 1 50-1000  5,00 1000 0,40 0,15 721006 721016
500 0,60 0,20
100 1,60 0,50
730101** 1 100-5000  10,0 5000 0,50 0,17 721006 721016
2500 0,80 0,20
500 1,60 0,40
730321 8 0,2-10  0,05 10 0,90 0,60 - -
5 1,50 0,90
1 4,00 3,50
730341 8 5-120  0,50 120 0,80 0,30 721008 721018
60 0,80 0,35
12 3,00 1,50
730361 8 10-300  1,00 300 0,70 0,20 721007 721017
150 0,90 0,30
30 3,00 1,20
730391 8 50-1200  5,00 1200 0,90 0,30 721006 721016
600 1,20 0,30
120 2,50 0,80
730421 12 0,2-10  0,05 10 0,90 0,60 - -
5 1,50 0,90
1 4,00 3,50
730441 12 5-120  0,50 120 0,80 0,30 721008 721018
60 0,80 0,35
12 3,00 1,50
730461 12 10-300  1,00 300 0,70 0,20 721007 721017
150 0,90 0,30
30 3,00 1,20
730491 12 50-1200  5,00 1200 0,90 0,30 721006 721016
600 1,20 0,30
120 2,50 0,80

All pipettes are supplied with a universal charger (EU, UK, US | JPN, AUS, KOR and CHN plugs)
* The listed systematic and random error values are valid for Pipetting (P) mode only and are achieved under strictly controlled conditions
during type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error values may
change without prior notice.
** NOTE: Minimum volume in P-mode is 500 µl. 100 µl is possible in multi-dispensing (d) mode.
 Pipette Tip Selection Guide Electronic Pipettes 25

⹅⹅ Pipette Tip Selection Guide

Optifit Tips, Non-filter* SafetySpace Filter Tips*
Electronic Pipettes

1000 WB

1200 Ext

10 Ext**
10 000
10 Ext

1000

1200

5000

1000
1200
10***

200
350

120
200
300
10*

20
Colour Volume
Code Range (µl)
Picus® &  0,2-10    
Picus® NxT 5-120
1-channel     
 10-300  
 50-1000   
 100-5000 
 500-10000 
®
Picus &  0,2-10    
Picus® NxT 5-120
8-channel    
 10-300  
 50-1200   
Picus® &  0,2-10    
Picus® NxT 5-120
12-channel    
 10-300  
 50-1200   
®
eLINE  0,1-5    
1-channel 0,2-10
    
 5-120   
 10-300  
 50-1000   
 100-5000 
eLINE®  0,2-10    
8-channel 5-120
   
 10-300  
 50-1200   
eLINE®  0,2-10    
12-channel 5-120
   
 10-300  
 50-1200   
* Note: Low Retention Tips are available in volumes up to 1200 µl.
** Filter tip with regular air gap
*** Extended inaccuracy and imprecision with pre-sterilized 10 µl tips
 Table of Contents Mechanical Pipettes 2727

Mechanical Pipettes
⹅⹅
Table of Contents

28 mLINE®
32 Proline® Plus
38 Proline®
42 Pipette Tip Selection Guide
28 Mechanical Pipettes mLINE®

mLINE® Mechanical Pipettes

⹅⹅
Effortless Accuracy

Sartorius’ most advanced mechanical Optiload- Loading Tips with

pipette family – the mLINE® – offers Minimum Force
excellent ergonomics, performance mLINE® pipettes have spring-loaded
and safety in manual pipetting. It is tip cones - the Optiload mechanism
designed to maintain high accuracy – on both single and multi-channel
and precision in repetitive and long- models. Optiload secures even tip
lasting manual pipetting. Its excellent sealing onto every individual tip
ergonomics minimize the risk of cone, and allows tips to be loaded
work related hand, arm and shoulder and ejected with minimum force.
mLINE®’s low pipetting forces disorders and Repetitive Strain Injury This is an advantage especially when
protect laboratory workers (RSI). working with multi-channel models
from injury. that otherwise would require more
It covers the full volume range of force for tip loading and ejection
0.1 µl to 10 ml and is available in than a single-channel model.
No rocking needed single- and multi-channel models.
Safe-Cone Filters Protect the
Excellent Ergonomics with Pipette
Low Pipetting Forces The replaceable Safe-Cone Filter
Excellent ergonomics and low located inside the tip-cone prevents
pipetting forces protect laboratory aerosols and fluids from penetrating
workers from injury. mLINE® has the pipette, also in cases of over-
exceptionally light pipetting and tip aspiration. The use of Safe-Cone
ejection forces due to its patented filters lengthens the maintenance
Optiload secures even tip spring mechanism. The light pipetting interval of the pipette.
sealing onto every individual
tip cone, and allows tips to force improves pipetting precision in
be loaded and ejected with long pipetting series. Safe-Cone Filters are available for all
minimum force. mLINE® models greater than 10 μl.
The starting forces in mLINE® pipettes They must be replaced regularly, and
are always constant, regardless of the always in cases of over-aspiration.
set volume. This improves pipetting Safe-Cone filters can easily and safely
results especially for small volumes. be ejected by removing the colour
cap and pressing down the operating
button.

Safe-Cone filters protect the

pipette from contamination,
and should be changed
regularly.
 mLINE® Mechanical Pipettes 29

⹅⹅
Features and Benefits
‒‒ Low pipetting forces that prevent ‒‒ Volume adjustment locking for ‒‒ Fully autoclavable without
RSI and improve results in long preventing accidental volume disassembly
pipetting series changes ‒‒ Simple to clean and maintain with
‒‒ Ergonomic finger support ‒‒ Easy-to-read volume display only three parts to disassemble
minimizes the grip force needed to ‒‒ Colour-coding of volumes to ease ‒‒ Easy user calibration e.g. for
hold the pipette the selection of corresponding different liquids
‒‒ Optiload mechanism in both pipette tips ‒‒ Materials have high chemical and
single- and multi-channel models ‒‒ Safe-Cone Filters available for UV-resistance to ensure a long life
for easy and light tip loading with models > 10 μl with convenient span for the pipette
perfect tip sealing filter ejection mechanism
‒‒ Single and multi-channel models
30 Mechanical Pipettes mLINE®

⹅⹅
Ordering Information
mLINE®

Order Channels Volume Colour- Increment Test Systematic Random Safe-Cone Filters
Code Range (µl) Coding (µl) Volume (µl) Error* Error* Standard Plus
(%) (%)
725010 1 0,1-3  0,002 3 1,30 0,80 - -
1,5 2,40 1,60
0,3 10,00 6,00
725020 1 0,5-10  0,01 10 1,00 0,60 - -
5 1,50 1,00
1 2,50 1,50
725030 1 2-20  0,02 20 0,90 0,40 721014 -
10 1,20 1,00
2 3,00 2,00
725050 1 10-100  0,10 100 0,80 0,15 721008 721018
50 1,00 0,40
10 2,00 1,00
725060 1 20-200  0,20 200 0,60 0,15 721007 721017
100 0,80 0,30
20 2,30 0,90
725070 1 100-1000  1,00 1000 0,70 0,20 721006 721016
500 0,70 0,20
100 2,00 0,50
725080 1 500-5000  10,0 5000 0,50 0,20 721005 721015
2500 0,60 0,30
500 2,00 0,60
725090 1 1-10 ml  20,0 10000 0,60 0,20 721005 721015
5000 1,20 0,30
1000 3,00 0,60
725120 8 0,5-10  0,01 10 1,50 1,00 - -
5 2,50 2,50
1 4,00 4,00
725130 8 5-100  0,10 100 0,70 0,25 721008 721018
50 1,00 0,70
10 3,00 1,50
725140 8 30-300  0,20 300 0,60 0,25 721007 721017
150 1,00 0,50
30 2,00 1,00
725220 12 0,5-10  0,01 10 1,50 1,00 - -
5 2,50 2,50
1 4,00 4,00
725230 12 5-100  0,10 100 0,70 0,25 721008 721018
50 1,00 0,70
10 3,00 1,50
725240 12 30-300  0,20 300 0,60 0,25 721007 721017
150 1,00 0,50
30 2,00 1,00

* The listed systematic and random error values are valid for pipetting mode only and are achieved under strictly controlled conditions
during type tests per ISO 8655.
Due to the continuous product development by Sartorius, the systematic and random error values may change without prior notice.
 mLINE® Multipacks Mechanical Pipettes 31

⹅⹅
mLINE® Multipacks
mLINE® Multipacks offer a set of our The Multipacks allow the affordable
premium mechanical pipettes in an renewal of existing pipettes, or to
affordable package, where a Linear set up new workstations, with a set
Stand and racks of matching tips are of highly ergonomic pipettes with
included. excellent accuracy and precision.

mLINE® Multipacks come

with a linear stand.

⹅⹅
Ordering Information
Order Code Item
LH-725661 mLINE® Pipette 3-pack 10
-- mLINE® pipettes: 0,5-10 µl, 10-100 µl and 100-1000 µl
-- Linear Stand
-- Matching Optifit Tip Single Tray rack (96 tips)
LH-725662 mLINE® Pipette 3-pack 20
-- mLINE® pipettes: 2-20 µl, 20-200 µl and100-1000 µl
-- Linear Stand
mLINE® Multipacks include
matching tips in single tray -- Matching Optifit Tip Single Tray rack (96 tips)
racks.
LH-725663 mLINE® Pipette 4-pack
-- mLINE® pipettes: 0,5-10 µl, 10-100 µl, 20-200 µl and
100-1000 µl
-- Linear Stand
-- Matching Optifit Tip Single Tray rack (96 tips)
LH-725664 mLINE® Pipette 5-pack
-- mLINE® pipettes: 2-20 µl, 10-100 µl, 20-200 µl,
100-1000 µl and 500-5000 µl
Linear Stand
-- Matching Optifit Tip Single Tray rack (96 tips)
LH-725665 mLINE® Pipette 3+1 -pack
mLINE® Pipette 5-pack
-- mLINE® pipettes: 0,5-10 µl, 20-200 µl, 100-1000 µl and
8-channel 30-300 µl
-- Linear Stand
-- Matching Optifit Tip Single Tray rack (96 tips)

mLINE® Pipette 3-pack 20

32 Mechanical Pipettes Proline® Plus

Proline® Plus Mechanical Pipettes

⹅⹅
Dependable Durability

Sartorius’ mechanical pipette Proline® Optiload allows tips to be loaded and

Plus is designed to offer comfort ejected with minimum force, which
and quality to manual pipetting. reduces the risk of hand injuries.
While it shares many of the excellent Moreover, it secures even tip sealing
features of mLINE®, it has a personal onto every individual tip cone.
design and a robust feel for heavier
use. In addition, it has the widest Protect the Pipette with
pipette range, including fixed volume Safe-Cone Filters
pipettes, for when volumes need to The replaceable Safe-Cone Filter
be ready-set to avoid errors. Proline® located inside the tip-cone prevents
Plus pipettes are an excellent choice aerosols and fluids from penetrating
for both experienced laboratory the pipette, also in case of over-
Proline® Plus has a
comfortable handle and an professionals and students. aspiration. The use of Safe-Cone
ergonomic finger support for Filters lengthens the maintenance
effortless pipetting. Ergonomic Design interval of the pipette. Safe-Cone
Proline® Plus has low pipetting Filters are available for all Proline®
forces, a comfortable handle and Plus models greater than 10 μl. They
an ergonomic finger support for must be replaced regularly, and
effortless pipetting. Good fit in hand always in case of over-aspiration.
minimizes the grip force needed to
hold the pipette, thereby reducing Easy Maintenance and Calibration
the risk of strain injury. No opening tools are needed for
cleaning and maintaining Proline®
Loading Tips on Multi-channel Plus pipettes, and only three parts
Pipettes with Minimum Force need to be cleaned. These pipettes
Loading and ejecting tips with multi- are also easy to calibrate using the
Safe-Cone filters protect the channel pipettes requires relatively calibration tool that is provided with
pipette from contamination,
high forces. Proline® Plus multi- the pipette.
and should be changed
regularly. channel pipettes have spring loaded
tip cones – the Optiload mechanism.

Proline® Plus pipettes have

only three parts that need
cleaning and maintenance .

Fully autoclavable
 Proline® Plus Mechanical Pipettes 33

⹅⹅
Features and Benefits
‒‒ Low pipetting forces that prevent ‒‒ Optiload mechanism in multi- fixed) to 10 ml
RSI and improve results in long channel models for easy and light ‒‒ Easy volume setting with click stop
pipetting series tip loading with perfect tip sealing mechanism
‒‒ Ergonomic finger support ‒‒ Wide variety of adjustable single ‒‒ Easy-to-read volume display
minimizes the grip force needed to and multi-channel models as well ‒‒ Colour-coding of different volumes
hold the pipette as fixed single-channel models to ease the selection of matching
‒‒ Volume range from 3 µl (5 µl for pipette tips
‒‒ Safe-Cone Filters available for
models > 10 μl
‒‒ Fully autoclavable without
disassembly
‒‒ Simple to clean and maintain with
only three parts to disassemble
‒‒ Easy user calibration e.g. for
different liquids
‒‒ Materials have high chemical and
UV-resistance to secure long life-
time of the pipette
34 Mechanical Pipettes Proline® Plus

⹅⹅
Ordering Information
Proline® Plus

Order Channels Volume Colour- Increment Test Systematic Random Safe-Cone Filters
Code Range (µl) Coding (µl) Volume (µl) Error* Error* Standard Plus
(%) (%)
728010 1 0,1 – 3  0,002 3 1,30 0,80 - -
1,5 2,40 1,60
0,3 10,00 6,00
728020 1 0,5 – 10  0,01 10 1,00 0,60 - -
5 1,50 1,00
1 2,50 1,50
728030 1 2 – 20  0,02 20 0,90 0,40 721014 -
10 1,20 1,00
2 3,00 2,00

728040 1 5 – 50  0,10 50 1,00 0,30 721008 721018

25 1,40 0,50
5 3,00 1,50
728050 1 10 – 100  0,10 100 0,80 0,15 721008 721018
50 1,00 0,40
10 2,00 1,00
728060 1 20 – 200  0,20 200 0,60 0,15 721007 721017
100 0,80 0,30
20 2,30 0,90
728070 1 100 – 1000  1,00 1000 0,70 0,20 721006 721016
500 0,70 0,20
100 2,00 0,50
728080 1 500 – 5000  10,0 5000 0,50 0,20 721005 721015
2500 0,60 0,30
500 2,00 0,60
728090 1 1 – 10 ml  20,0 10000 0,60 0,20 721005 721015
5000 1,20 0,30
1000 3,00 0,60
728120 8 0,5 – 10  0,01 10 1,50 1,00 - -
5 2,50 2,50
1 4,00 4,00
728130 8 10 – 100  0,10 100 0,70 0,25 721008 721018
50 1,00 0,70
10 3,00 1,50
728140 8 30 – 300  0,20 300 0,60 0,25 721007 721017
150 1,00 0,50
30 2,00 1,00
728220 12 0,5 – 10  0,01 10 1,50 1,00 - -
5 2,50 2,50
1 4,00 4,00
728230 12 10 – 100  0,10 100 0,70 0,25 721008 721018
50 1,00 0,70
10 3,00 1,50
728240 12 30 – 300  0,20 300 0,60 0,25 721007 721017
150 1,00 0,50
30 2,00 1,00

* The listed systematic and random error values are valid for pipetting mode only and are achieved under strictly controlled conditions
during type tests per ISO 8655.
Due to the continuous product development by Sartorius, the systematic and random error values may change without prior notice.
 Proline® Plus Mechanical Pipettes 35

⹅⹅
Ordering Information

Proline® Plus FIXED Volume, Single-Channel

Order Channels Volume Colour- Test Systematic Random Safe-Cone Filters

Code (µl) Coding Volume Error* Error* Standard Plus
(µl) (%) (%)
728515 1 5  5 1,30 1,20 – –
728520 1 10  10 0,80 0,80 – –
728530 1 20  20 0,60 0,50 721014 –
728535 1 25  25 0,50 0,30 721008 721018
728545 1 50  50 0,50 0,30 721008 721018
728550 1 100  100 0,50 0,30 721008 721018
728560 1 200  200 0,40 0,20 721007 721017
728565 1 250  250 0,40 0,20 721006 721016
728567 1 500  500 0,30 0,20 721006 721016
728570 1 1000  1000 0,30 0,20 721006 721016
728575 1 2000  2000 0,30 0,15 721005 721015
728580 1 5000  5000 0,30 0,15 721005 721015
728590 1 10 ml  10000 0,60 0,20 721005 721015

* The listed systematic and random error values are valid for pipetting mode only and are achieved under strictly controlled
conditions during type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error
values may change without prior notice.
36 Mechanical Pipettes Proline® Plus

⹅⹅
Proline® Plus Starter Kits and 5-Pack
Proline® Plus Starter Kit offers an The Proline® Plus 5-Pack is an
opportunity to test and get started affordable choice for renewing
with Proline® Plus. You can choose mechanical pipettes or setting up
between four Starter Kits, with two new workstations. The pack includes
or three single-channel Proline® Plus five pipettes, a Carousel Stand, 200
pipettes. All kits include a range of µl Optifit Tips (11 x 96 tips) and an
useful accessories, such as a pipette Elbow Pad – all ready for work to
holder, and calibration tool that also begin.
The Proline® Plus 5-Pack
comes with a Carousel Stand acts as a tube opener.

⹅⹅
Ordering Information
Proline® Plus Starter Kits and 5-Pack

Order Code Item

728650 Proline® Plus Starter Kit 1
-- Proline® Plus pipettes: 0,1-3 µl and 0,5-10 µl
-- Matching Optifit Tip Single Tray rack (96 tips)
-- Pipette Holder + 2
-- Calibration Tool | Tube Opener + 2
The Proline® Plus Starter Kits
and 5-Pack include matching 728651 Proline® Plus Starter Kit 2
tips in single tray racks. -- Proline® Plus pipettes: 0,5-10 µl, 10-100 µl and 100-1000 µl
-- Matching Optifit Tip Single Tray rack (96 tips)
-- Pipette Holder x 3
-- Calibration Tool | Tube Opener + 3
728652 Proline® Plus Starter Kit 3
-- Proline® Plus pipettes: 2-20 µl, 20-200 µl and 100 1000 µl
-- Matching Optifit Tip Single Tray rack (96 tips)
-- Pipette Holder x 3
-- Calibration Tool | Tube Opener + 3
728653 Proline® Plus Starter Kit 4
-- Proline Plus pipettes: 500-5000 µl and 1000-10000 µl
-- Matching Optifit Tips: 5 ml Bulk (100 tips), 10 ml Bulk (50 tips)
-- Pipette Holder + 2
Pipette Holder -- Calibration Tool | Tube Opener + 2
LH-728654 Proline® Plus 5-Pack
-- Proline Plus pipettes: 0,5-10 µl, 10-100 µl, 20-200 µl,
100-1000 µl and 500-5000 µl
-- Carousel Stand
-- Elbow Pad
-- Matching Optifit Tip Single Tray rack (96 tips), and
Refill Tower (10 x 96 tips)

Calibration Tool or Tube

Opener
Proline® Plus Mechanical Pipettes 37
38 Mechanical Pipettes Proline®

Proline® Mechanical Pipettes

⹅⹅
Affordable Reliability

The fact that Proline®, our first Due to its relatively light weight, high
mechanical pipette, is still in use accuracy and precision, it is also used
in many laboratories by over 200 by many professionals.
000 users, testifies to its timeless,
practical design and reliability. Proline® can be used with many
Being the most affordable pipette universal tip brands and the range
in Sartorius’ range of mechanical includes also fixed volume models,
pipettes, it is ideal for universities and which makes it a flexible tool for
colleges, or any laboratory seeking a various laboratories.
cost-efficient liquid handling tool.

⹅⹅
Features and Benefits
‒‒ Wide range of pipettes, both
adjustable and fixed volume
‒‒ Volume range from 2,5 µl (5 µl for
fixed) to 5 ml
‒‒ Easy volume setting with click stop
mechanism
‒‒ Safe-Cone Filters available for
models > 10 μl
‒‒ Easy user calibration e.g. for
different liquids
39
40 Mechanical Pipettes Proline®

⹅⹅
Ordering Information
Proline®

Order Code Channels Volume Colour- Increment Test Systematic Random Safe-Cone Filters
Range (µl) Coding (µl) Volume (µl) Error* Error* Standard Plus
(%) (%)
720010 1 0,1 – 2,5  0,05 2,5 2,50 2,00 – –
1,25 3,00 3,00
0,25 12,00 6,00
720015 1 0,5 – 10  0,10 10 1,00 0,80 – –
5 1,50 1,50
1 2,50 1,50
720080 1 2 – 20  0,50 20 0,90 0,40 721008 721018
10 1,20 1,00
2 3,00 2,00
720025 1 5 – 50  0,50 50 0,60 0,30 721008 721018
25 0,90 0,60
5 2,00 2,00
720050 1 10 – 100  1,00 100 0,80 0,20 721007 721017
50 1,00 0,40
10 3,00 1,00
720070 1 20 – 200  1,00 200 0,60 0,20 721007 721017
100 0,80 0,30
20 2,50 0,80
720060 1 100 – 1000  5,00 1000 0,60 0,20 721006 721016
500 0,70 0,25
100 2,00 0,70
720110 1 1000 – 5000  50,0 5000 0,50 0,20 721005 721015
2500 0,60 0,30
1000 0,70 0,30
720210 8 0,5 – 10  0,10 10 1,50 1,50 – –
5 2,50 2,50
1 4,00 4,00
720220 8 5 – 50  0,50 50 1,00 0,50 721014 –
25 1,50 1,00
5 3,00 2,00
720240 8 50 – 300  5,00 300 0,70 0,25 721014 –
150 1,00 0,50
50 1,50 0,80
720310 12 0,5 – 10  0,10 10 1,50 1,50 – –
5 2,50 2,50
1 4,00 4,00
720320 12 5 – 50  0,50 50 1,00 0,50 721014 –
25 1,50 1,00
5 3,00 2,00
720340 12 50 – 300  5,00 300 0,70 0,25 721014 –
150 1,00 0,50
50 1,50 0,80

* The listed systematic and random error values are valid for pipetting mode only and are achieved under strictly controlled conditions
during type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error values may
change without prior notice.
 Proline® FIXED Volume Mechanical Pipettes 41

⹅⹅
Ordering Information
Proline® FIXED Volume

Order Code Channels Volume Colour- Test Systematic Random Safe-Cone Filters
(µl) Coding Volume (µl) Error* Error* Standard Plus
(%) (%)
722001 1 5  5 1,30 1,20 – –
722004 1 10  10 0,80 0,80 – –
722010 1 20  20 0,60 0,50 721008 721018
722015 1 25  25 0,50 0,30 721008 721018
722020 1 50  50 0,50 0,30 721008 721018
722025 1 100  100 0,50 0,30 721007 721017
722030 1 200  200 0,40 0,20 721007 721017
722035 1 250  250 0,40 0,20 721006 721016
722040 1 500  500 0,30 0,20 721006 721016
722045 1 1000  1000 0,30 0,20 721006 721016
722050 1 2000  2000 0,30 0,15 721005 721015
722055 1 5000  5000 0,30 0,15 721005 721015

* The listed systematic and random error values are valid for pipetting mode only and are achieved under strictly controlled
conditions during type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random
error values may change without prior notice.
42 Mechanical Pipettes Pipette Tip Selection Guide

Pipette Tip Selection Guide

⹅⹅

Optifit Tips, Non-filter* SafetySpace Filter Tips*

Mechanical Pipettes

1000 WB

10 Ext**
10 000
10 Ext

1000

5000

1000
10***

10***
200

350

120

200

300
20
Colour Code µl
mLINE®  0,1-3    
1-channel 0,5-10
    
 2-20  
 10-100   
 20-200    
 100-1000   
 500-5000 
 1-10 ml 
mLINE®  0,5-10    
8-channel 5-100
   
 30-300  
mLINE®  0,5-10    
12-channel 5-100
   
 30-300  
Proline® Plus  0,1-3    
1-channel 0,5-10
    
 2-20  
 5-50  
 10-100   
 20-200    
 100-1000   
 500-5000 
 1-10 ml 
Proline® Plus  0,5-10    
8-channel 10-100
   
 30-300  
Proline® Plus  0,5-10    
12-channel 10-100
   
 30-300  
* Note: Low Retention Tips are available in volumes up to 1200 µl.
** Filter tips with regular air gap
*** Extended inaccuracy and imprecision with pre-sterilized 10 μl tips
 Pipette Tip Selection Guide Mechanical Pipettes 43

Optifit Tips, Non-filter* SafetySpace Filter Tips*

Mechanical Pipettes

1000 WB

10 Ext**
10 000
10 Ext

1000

5000

1000
10***

10***
200

350

120

200

300
20
Colour Code µl
Proline® Plus  5    
Fixed Volume 10
    
1-channel
 20  
 25  
 50  
 100   
 200    
 250   
 500   
 1000   
 2000 
 5000 
 10 ml 
Proline®  0,1-2,5    
1-channel 0,5-10
      
 2-20   
 5-50   
 10-100     
 20-200    
 100-1000   
 1000-5000 
Proline®  0,5-10    
8-channel 5-50
     
 50-300  
Proline®  0,5-10    
12-channel 5-50
     
 50-300  
Proline®  5      
Fixed Volume 10
      
1-channel
 20    
 25   
 50   
 100     
 200    
 250   
 500   
 1000   
 2000 
 5000 
 Table of Contents Stands & Accessories 4545

Stands & Accessories

⹅⹅
Table of Contents

46 Pipette Stands
47 Elbow Pad
48 Safe-Cone Filters
50 Reagent Vessel
50 Calibration Tool|Tube Opener &
Colour-coding Caps
46 Stands & Accessories Pipette Stands

Pipette Stands
⹅⹅

When the pipette is not in The Linear Stand is designed for all
use, it should be stored in an Sartorius mechanical and electronic
upright position in order to avoid pipettes, particularly for mLINE®,
contamination from work surfaces. Proline® Plus and Proline® mechanical
Sartorius provides stands for all devices. This stand is also compatible
of its pipettes. It is recommended with a wide range of other pipette
that electronic pipettes be stored makes.
and charged on a charging stand
whenever they are not in use. In this The simplest of all are the pipette
Charging Carousel Stand way, their batteries always remain holders which are attached to the
charged for when work begins. front edge of a shelf. These are
suitable for mechanical pipettes.
Compact carousel stands are ideal for
saving bench space. There is one for
mechanical pipettes, and a charging
carousel stand for electronic pipettes.

⹅⹅
Ordering Information
Pipette Stands

Charging Stand
Order Code Item
730981 Charging Stand for one electronic pipette*
730991 Charging Carousel for 4 electronic pipettes*
725620 Linear Stand for all Sartorius pipette models
725600 Carousel Stand for 6 mechanical pipettes
725610 Holder for one mLINE® | Proline® Plus pipette
721259 Holder for Proline® pipette

* Supplied with a universal charger with EU, UK, US|JPN, AUS, KOR and CHN plugs.

Linear Stand (non-charging)

Non-charging Carousel Stand Pipette Holder Pipette Holder

-mLINE® | Proline® Plus Pipettes -Proline® Pipettes
 Elbow Pad Stands & Accessories 47

Elbow Pad
⹅⹅

The Elbow Pad provides comfort ⹅⹅

Features and Benefits
while pipetting. The visco-elastic
‒‒ Improves pipetting ergonomics
material of the pad relieves contact
‒‒ Conforms to any elbow size
stress, pain and discomfort under the
or shape
elbow.
‒‒ Coating is pleasant to the skin
‒‒ The compact size takes up little
The Elbow Pad is Ideal for
bench space
‒‒ long periods of pipetting
‒‒ Very durable
‒‒ work requiring high concentration,
‒‒ Easy to clean with washing up
e.g. micro plate work
liquid, or ethanol (70%)
‒‒ any work where a cushion beneath
‒‒ Not autoclavable
the elbow or wrist is needed

⹅⹅
Ordering Information
Elbow pad

Order Code Item Qty

723103 Elbow Pad 1
48 Stands & Accessories Safe-Cone Filters

Safe-Cone Filters
⹅⹅

Why Should You Use Safe-Cone How Often Should You Change?
Filters? The interval of changing the
These unique and replaceable filter depends completely on the
polyethylene (PE) filters act as a final application and the sample. However,
barrier to prevent any fluids and according to studies, the filter is
liquid vapours from reaching the recommended to be changed daily
internal components of the pipette. (after 50 to 250 pipettings) and
immediately in case of over-aspi­
‒‒ Protect the pipette and sample ration.
from contamination
Tweezers for exchanging ‒‒ Prolong the pipette’s lifetime How to Change?
filters in pipettes are ‒‒ Reduce maintenance intervals
supplied with all pipettes To ensure that the user is protected
excluding mLINE®. ‒‒ Are cost-effective compared to from contamination, tweezers should
filter tips be used when removing used filters
from the pipette tip cone. The mLINE®
When Should You Use them? also features a built-in filter ejector.
The ultimate pipette protectors are In addition, the tip cone should be
available in two types: cleaned with ethanol (70%) prior to
the insertion of a new filter.
Plus Filter
For more demanding applications
such as radioactive work, cell culture,
bacterial and virological work and
molecular biology.
Built-in filter ejector Standard Filter
in mLINE® For general applications. Can be used
in same type of work as the Plus
filter, but needs to be changed more
frequently.
 Safe-Cone Filters Stands & Accessories 49

⹅⹅
Contamination in Pipette Barrel

Bacterial Growth

Number of pipettings
Pipette contamination in pipette barrel when pipetting liquid culture of
bacteria Micrococcus Luteus.

⹅⹅
Ordering Information
Safe-Cone Filters

Order Code Item Qty/Unit

721008 Standard d 2,51 mm PE 50
721007 Standard d 3,15 mm PE 50
721006 Standard d 5,33 mm PE 50
721005 Standard d 6,73 mm PE 50
721014 Standard d 1,83 mm PE 50
721018 Plus d 2,51 mm PE 50
721017 Plus d 3,15 mm PE 50
721016 Plus d 5,33 mm PE 50
721015 Plus d 6,73 mm PE 50

PE = polyethylene
50 Stands & Accessories Reagent Vessel | Calibration Tool

Reagent Vessel
⹅⹅

Made from polypropylene, the

autoclavable and durable reagent
vessel is chemically resistant to all
common reagents.

Fully autoclavable

⹅⹅
Ordering Information
Reagent Vessel

Order Code Item Qty

783500 Reagent Vessel (capacity 120 ml) 16

Calibration Tool|Tube Opener & Colour-coding Caps

⹅⹅

The Calibration tool|Tube opener

is used for calibrating mLINE® and
Proline® Plus pipettes.

The Colour-coding Caps can be used

for personalizing the mLINE® pipette. Fully autoclavable

⹅⹅
Ordering Information
Calibration Tool|Tube Opener and Colour-coding Caps

Order Code Item Qty

726203 Calibration Tool | Tube Opener 1
726001 Colour-coding Caps for mLINE® 5
 Table of Contents Pipette Tips 5353

Pipette Tips
⹅⹅
Table of Contents

54 Pipette Tips
56 Packaging Options
58 Optifit Tips
60 SafetySpace Filter Tips
61 Low Retention Tips
62 Ordering Information
54 Pipette Tips Optifit Tips and SafetySpace Filter Tips

Pipette Tips
⹅⹅
The Perfect Match for Your Pipette

In liquid handling, accurate pipetting The unique Optiload mechanism of

results are not dependent on our pipettes allows tip attachment
the pipette or the tip alone, but and loading with reduced force, but
a combination of these and the with complete sealing, which is vital
comfort of the user. Our non-filter to get accurate results.
Optifit Tips and SafetySpace filter tips
are designed and manufactured as a Sartorius tips correspond with
perfect fit for our pipettes, enabling the colour-coding of Sartorius
maximal accuracy, precision and pipettes, to allow easy matching of
ergonomics. Moreover, well-matching corresponding volumes.
tips protect the pipette tip cone from
Optiload for a tight fit and wear and tear.
equal sealing on every
channel’s tip cone.

⹅⹅
Premium Quality and Purity
Manufacturing the tips in our own management system follows not only
production facility allows us to ISO 9001 and ISO 14001, but also
maintain the highest quality and ISO 13485. Tip production also abides
purity standards, by selecting the by the ISO 14644-1 standard, in
best plastic materials and controlling order to fulfil ISO Class 8 cleanroom
the manufacturing process from conditions for secured tip purity.
beginning to end. Our quality

The factory in Kajaani,

Finland.

⹅⹅
Contamination Free Tips
To avoid contamination through Additionally, our highly experienced
human contact, we have automated and trained personnel are equipped
the entire tip manufacturing process. with specially designed coveralls,
Pure virgin polypropylene (PP) masks, hair nets and gloves, in
plastic is automatically fed from silos order to further diminish risks of
into moulding machines. Moulding contamination.
machines and robots located in
isolated clean cells, load the tips An independent laboratory checks
automatically into tip trays and each Single Tray and Refill Pack tip
packaging. HEPA filters and higher lot for RNase, DNase and endotoxins.
air pressure are applied for purity Lot-specific purity certificates can
within the cell. All Sartorius Single be downloaded from www.sartorius.
Tray tip racks, Single Refill Packs and com (select from the navigation bars:
FlexiBulk packs are individually and -> Liquid Handling -> Tip Purity
Lot-specific purity certificate automatically packed in air-tight Certificates).
plastic, in order to rule out any
danger of contamination.
 Optifit Tips and SafetySpace Filter Tips Pipette Tips 55 55

Definitions:

DNase A deoxyribonuclease (DNase) is any enzyme that catalyzes the

degradation of DNA. The absence of DNase is tested by using
fluorometric assay. The detection level of the assay is 6,25*
10-5 U/µl when DNase I is used as a standard.
RNase Ribonuclease (RNase) is an enzyme that catalyzes the
degradation of RNA into smaller components and can be
generally found from organisms. The absence of RNase is tested
by using fluorometric assay. The detection level of the assay is
3,125* 10-9 U/µl, when RNase A is used as a standard.
Endotoxins Endotoxins are lipopolysaccharides found in the Gram-negative
bacteria and can cause several serious health effects in humans
and animals. Limulus Amebocyte Lysate (LAL) Gel Clot method
is used to detect the presence of endotoxins on the pipette tips.
The detection level of the LAL assay is 0.03 IU/ml (EU/ml).
Sterilization The destruction of all microbial life, including bacterial
endospores. Can be accomplished e.g. using steam, heating,
chemicals, or radiation. We use e-beam irradiation.

⹅⹅
Features and Benefits
Best Fit – Highest Possible Accuracy Premium Quality and Purity:
‒‒ Perfect fitting and sealing with ‒‒ Strict quality standards, ISO 9001
Sartorius pipettes secure the and ISO 13485, are followed from
highest possible accuracy and R&D to production and delivery
precision ‒‒ Manufactured in ISO 8 classified
‒‒ Compatible with Optiload feature clean room conditions
in Sartorius Picus® NxT, Picus®, ‒‒ Manufacturing process free of
eLINE®, mLINE® and Proline® Plus DNase, RNase and endotoxins:
pipettes enabling ergonomic and Single Trays,Refill Packs and
light tip attachment and ejection FlexiBulk packs certified pure by lot
‒‒ Colour-coding of tip trays number
allows easy matching with a ‒‒ Pre-sterilised tips are e-beam
corresponding colour-coded irradiated
Sartorius pipette ‒‒ All tip packages, including
‒‒ Compatible with most other individual racks, are lot numbered
pipette makes for full traceability
‒‒ The highest quality virgin
polypropylene used as raw material
⹅⹅
Tip Selection Guide per Application
Tip Type Optifit Tips SafetySpace Low
Filter Tips Retention
Tips
Purity Standard Free of DNase, Pre-sterilized Pre-sterilized & free
RNase & & free of of DNase, RNase and
endotoxins DNase, RNase endotoxins
and endotoxins
Regular pipetting applications 
Applications where prevention
  
of cross-contamination is vital
Pipetting liquids with low
surface tension 
(e.g. detergents, solvents)
56 Pipette Tips Packaging Options

Packaging Options
⹅⹅

⹅⹅
Racked Tips
Single Tray Rack ‒‒ Air-tight plastic wrapping around
‒‒ 96 tips in convenient and reusable the rack secures purity during
tray racks (sales unit contains transport and storage (wrapping is
10 tray racks, total 960 tips) regular waste)
‒‒ Certified free of DNase, RNase and ‒‒ Tip trays are colour-coded to
endotoxins indicate the matching, colour-
‒‒ e-beam pre-sterilized option coded Sartorius pipette
available ‒‒ Covers a large range of tip volumes
‒‒ Lot-specific purity certificates can from 10 μl to 5 ml
be downloaded from ‒‒ Fully autoclavable at 121°C for
www.sartorius.com 20 minutes
‒‒ Informative rack labelling: ‒‒ Tray racks can be easily reloaded
volume, product number, lot with Refill tips
number improves tip identification ‒‒ Racks, trays and tips are 100%
Single tray racks and traceability recyclable polypropylene (PP)

⹅⹅
Refill Tips
Refill Tower ‒‒ Covers the most widely used tip
‒‒ Space-saving with 10x96 tips in sizes:
one tower 10 μl, 200 μl and 350 μl
‒‒ Tip trays are compatible with ‒‒ Trays and tips are fully
Single Tray racks for convenient autoclavable at 121°C for 20
use minutes
‒‒ Trays are colour-coded to indicate ‒‒ 100% recyclable cardboard
the matching, colour-coded packaging, and plastic (PP) trays
Sartorius pipette and tips

Refill towers
 Packaging Options Pipette Tips 57

Single Refill Packs ‒‒ Informative rack labelling: volume,

‒‒ 10, 15 or 20 trays of 96 tip trays, product number, lot number
depending on tip volume improves tip identification and
‒‒ Certified free of DNase, RNase and traceability
endotoxins ‒‒ Tip trays are colour-coded to
‒‒ e-beam pre-sterilized option indicate the matching, colour-
available coded Sartorius pipette
‒‒ Lot-specific purity certificates ‒‒ Covers a large range of tip volumes
can be downloaded from www. from 10 μl to 1200 μl
sartorius.com ‒‒ Trays and tips are fully
Single refill packs ‒‒ Individually packed air-tight tip autoclavable at 121°C for 20
trays for maximum purity with less minutes
packaging material compared to ‒‒ 100% recyclable trays and tips (PP).
racked tips Container lid is regular waste.
‒‒ Tip trays are compatible with
Single Tray racks for convenient
use

⹅⹅
Bulk Tips
FlexiBulk ‒‒ Certified free of DNase, RNase and
‒‒ Tips made to the Sartorius quality endotoxins
standard in economical packaging ‒‒ Lot-specific purity certificates
‒‒ Packed orderly in compact re- can be downloaded from www.
sealable plastic packages (480 or sartorius.com
960pcs depending on tip volume) ‒‒ Tips are fully autoclavable at 121°C
‒‒ Covers a large range of tip volumes for 20 minutes
from 200 μl to 1200 μl ‒‒ 100% recyclable tips (PP) and
package (PET)

FlexiBulk

Bulk in a Box ‒‒ Covers tip volumes 10 µl, 5 ml and

‒‒ Tips made to the Sartorius quality 10 ml
standard in economical packaging ‒‒ Tips are fully autoclavable at 121°C
‒‒ Packed in re-sealable bags in for 20 minutes
cardboard boxes (100, 250 or ‒‒ 100% recyclable tips and package
1000 pcs depending on tip
volume)

Bulk in a box
58 Pipette Tips Optifit Tips

Optifit Tips
⹅⹅
Standard Tips for Various Needs

Sartorius Optifit tips are an excellent The Single Tray tip racks are
choice for various laboratories and ideal for easy tip loading and for
pipetting tasks with their wide contamination-free pipetting. In
packaging and purity options. The order to reuse the empty tip racks
Optifit tips are packed in single and to create less waste, you may
tray racks, refill towers, single refill fill the empty racks with Refill tips,
packs, and bulk packages. Optifit either using the refill tower or purity
tips are available DNase, RNase and certified refill pack tips. The FlexiBulk
endotoxin free, as well as e-beam tips are the choice, if you need a
pre-sterilized. cost-effective, yet purity certified
solution, in bulk tip format.

⹅⹅
Available Packaging Options
‒‒ Single tray rack
‒‒ Refill tower
‒‒ Refill pack
‒‒ FlexiBulk pack
‒‒ Bulk in a box

See details on packaging options on

pages 56 and 57.

⹅⹅
Features and Benefits
‒‒ Standard non-filter tips made to ‒‒ Wide selection of packaging and
Sartorius quality standards purity options
‒‒ Low Retention Tip range also ‒‒ Available as DNase, RNase and
available for liquids with low endotoxin-free
surface tension ‒‒ e-beam pre-sterilized packaging
‒‒ Perfect fitting and sealing with options available
Sartorius Picus® NxT, Picus®, eLINE®, ‒‒ Full traceability
mLINE®, Proline® Plus, and Proline® ‒‒ Colour-coded trays to match with
pipettes corresponding Sartorius pipettes
‒‒ Wide tip volume range from 10 µl ‒‒ Fully autoclavable at 121°C for
to 10 ml 20 minutes
Optifit Tips Pipette Tips 59
60 Pipette Tips SafetySpace Filter Tips

SafetySpace Filter Tips

⹅⹅
Protect Valuable Samples

SafetySpace filter tips, made of virgin The unique feature with SafetySpace
polypropylene, feature filter barriers Filter Tips is the additional space
that effectively capture solid and left between the sample and the
liquid aerosol particles. The filter filter that conventional filter
is made of polyethylene without tips do not have. This extra space
self-sealing additives to avoid any prevents the liquid from touching,
interference with the sample and and permeating, the filter and thus
results. The filter protects the sample guarantees the pipetting accuracy.

] Safety air
gap
against contamination. In addition, it
protects the pipette and prolongs the
maintenance interval of the pipette.
Any liquid types and pipetting
techniques can be applied without
the risk of the liquid permeating the
filter.
The SafetySpace Filter Tips are Ideal
for The Extra Space is Particularly
‒‒ molecular biology Useful in the Following
‒‒ microbiology Applications:
‒‒ cell culture applications ‒‒ pipetting foaming liquids such as
‒‒ radioactive work buffers and proteins
‒‒ pipetting solvents
‒‒ multiple dispensing functions of
electronic pipettes
‒‒ reverse pipetting

⹅⹅
Available Packaging Options
‒‒ Single tray rack

⹅⹅
Features and Benefits
‒‒ Filter minimizes the risk of aerosol ‒‒ e-beam pre-sterilized
contamination ‒‒ Full traceability
‒‒ Large empty volume between the ‒‒ Colour-coded trays indicate the
sample and filter prevents liquid matching colour-coded Sartorius
from contacting the filter pipette
‒‒ Covers tip volumes from 10 μl to
1200 μl
‒‒ Certified free of DNase, RNase and
endotoxins
 Low Retention Tips Pipette Tips 61

Low Retention Tips

⹅⹅
Ensure Optimal Sample Recovery

Pipetting liquids that contain Low Retention Tips maximize the

detergents can be problematic when sample recovery, when handling
using standard pipette tips. Some detergent containing or other
liquid residue often remains in the tip liquids with low surface tension.
due to differences in surface energies Better reproducibility in pipetting
between the plastic pipette tip is especially beneficial in sensitive
and the sample. The residue causes molecular biology applications, where
imprecision in pipetting and loss of reagents often contain detergents,
valuable samples or reagents. for example in:
‒‒ PCR, real-time PCR
We use an advanced technology to ‒‒ Cloning, sequencing and other DNA
The four tips on the right are manufacture Low Retention Tips & RNA techniques
low retention tips, providing
maximum sample recovery. that have an extremely even and ‒‒ SDS-PAGE and other protein
durable hydrophobic surface. Unlike analysis methods
some other hydrophobic tips on the ‒‒ Protein purification techniques
market, our low retention tips do not
contain any leachables that might
risk your sample.

⹅⹅
Available Packaging Options
‒‒ Single tray rack
‒‒ Refill tower

⹅⹅
Features and Benefits
Packaging options for Low ‒‒ Extremely hydrophobic tips surface packaging options available
Retention tips. ‒‒ Maximum sample recovery for ‒‒ e-beam pre-sterilized packaging
fluids with low surface tension options available
‒‒ Durable, high chemical resistance, ‒‒ Full traceability
ention
no leachables ‒‒ Colour-coded trays indicate the
Ret
‒‒ Covers tip volumes from 10 μl to matching colour-coded Sartorius
Low

Tip

1200 μl pipette
s

LowRet
‒‒ Filter (SafetySpace) and non-filter ‒‒ Non-filter tips are fully
Sa (Optifit) tip options are available autoclavable at 121°C for
rt o rius
‒‒ DNase, RNase and endotoxin-free 20 minutes
62 Pipette Tips Ordering Information

⹅⹅
Ordering Information

Optifit Tips

Volume Range Length Packaging Low Purity Level Tips/Unit Order Code
Retention
Free of Pre-
RNase, steri-
DNase, lised
endotoxins
 0,1-10 µl 31,5 mm Single Tray • 10 x 96 790010
Single Tray • • 10 x 96 LH-L790010
Single Tray • • 10 x 96 790011
Refill Tower 10 x 96 790012
Refill Tower • 10 x 96 LH-L790012
Refill Pack • • 20  x  96 790013
Bulk in Box 1000 790014
 0,1-10 µl Extended 46 mm Single Tray • 10 + 96 783210
Single Tray • • 10 + 96 783211

 0,5-200 µl 51 mm Single Tray • 10 x 96 790200

Single Tray • • 10 x 96 LH-L790200
Single Tray • • 10 x 96 790201
Refill Tower 10 x 96 790202
Refill Tower • 10 x 96 LH-L790202
Refill Pack • • 15 x 96 790203
FlexiBulk • 960 LH-B790204
 5-350 µl 54 mm Single Tray • 10 x 96 790350
Single Tray • • 10 x 96 LH-L790350
Single Tray • • 10 x 96 790351
Refill Tower 10 x 96 790352
Refill Tower • 10 x 96 LH-L790352
Refill Pack • • 15 x 96 790353
FlexiBulk • 960 LH-B790354
 10-1000 µl 71,5 mm Single Tray • 10 x 96 791000
Single Tray • • 10 x 96 LH-L791000
Single Tray • • 10 x 96 791001
Refill Pack • 10 x 96 791002
Refill Pack • • 10 x 96 791003
FlexiBulk • 480 LH-B791004

For your guidance the tips are illustrated in the actual size.

Empty Tip Boxes for Refill System (Tips and Trays are Not Included)

Item Tip Type (Non-filter Tips) Racks/Unit Order Code

Empty Tip Box for Refill System 10, 200, 350 µl 10 790910
Empty Tip Box for Refill System 1000, 1200 µl 10 790920
 Ordering Information Pipette Tips 63

Volume Range Length Packaging Low Purity Level Tips/Unit Order Code
Retention
Free of Pre-
RNase, steri-
DNase, lised
endotoxins
 10-1000 µl Wide bore tip 68,5 mm Single Tray • 10 x 96 791020
Single Tray • • 10 x 96 791021
FlexiBulk • 480 LH-B791024

 50-1200 µl 71,5 mm Single Tray • 10 x 96 791200

Single Tray • • 10 x 96 LH-L791200
Single Tray • • 10 x 96 791201
Refill Pack • 10 x 96 791202
Refill Pack • • 10 x 96 791203
FlexiBulk • 480 LH-B791204

 50-1200 µl Extended 90 mm Single Tray • 10 x 96 791210

Single Tray • • 10 x 96 LH-L791210
Single Tray • • 10 x 96 791211
Refill Pack • 10 x 96 791212
Refill Pack • • 10 x 96 791213

 100-5000 µl 150 mm Single Tray • 50 780304

Single Tray • • 50 780305
Bulk in Box 100 780300
Bulk in Carton 1000 780308

 1-10 ml 155 mm Bulk in Box 250 LH-780316

Note: The ordering information for 10 ml tip for MidiPlus can be found on page 69.

NOTE! Tip compatibility with pipettes are shown in the Tip Selection Guides at
Electronic Pipettes, page 25, and Mechanical Pipettes, pages 42 - 43.
64 Pipette Tips Ordering Information

SafetySpace Filter Tips

Volume Range Length Packaging Low Purity Level Tips/Unit Order Code
Retention
RNase, Pre-
DNase, steri-
endotoxin- lised
free
0,1-10 µl 31,5 mm Single Tray • • 10 x 96 790011F

Single Tray • • • 10 x 96 LH-LF790011

 0,5-20 µl 51 mm Single Tray • • 10 x 96 790021F

Single Tray • • • 10 x 96 LH-LF790021

 2-120 µl 51 mm Single Tray • • 10 x 96 790101F

Single Tray • • • 10 x 96 LH-LF790101

 5-200 µl 52,5 mm Single Tray • • 10 x 96 790201F

Single Tray • • • 10 x 96 LH-LF790201

 5-300 µl 52,5 mm Single Tray • • 10 x 96 790301F

Single Tray • • • 10 x 96 LH-LF790301

 50-1000 µl 78 mm Single Tray • • 10 x 96 791001F

Single Tray • • • 10 x 96 LH-LF791001

 50-1200 µl 90 mm Single Tray • • 10 x 96 791211F

Single Tray • • • 10 x 96 LH-LF791211

For your guidance the tips are illustrated in the actual size.
Filter tips are not recommended to be used simultaneously with Safe-Cone Filters
 Ordering Information Pipette Tips 65

Filter Tips with Regular Air Gap

Volume Range Length Packaging Low Purity Level Tips/Unit Order Code
Retention
RNase,
DNase, Pre-
endotoxin- steri-
free lised
 0,1-10 µl 46 mm Single Tray • • 10x96 783201

NOTE! Tip compatibility with pipettes are shown in Tip Selection Guides at
Electronic Pipettes, page 25, and Mechanical Pipettes, pages 42 - 43.
 Table of Contents Maxi-volume Liquid Handling 67 67

Maxi-volume Liquid Handling

⹅⹅
Table of Contents

68 Midi Plus Pipetting Controller

70 Proline® Prospenser Bottle-top Dispenser
71 Prospenser Bottle-top Dispenser
72 Biotrate Digital Burettes
73 Ordering Information and Performance Specifications
68 Maxi-volume Liquid Handling Midi Plus

Midi Plus Pipetting Controller

⹅⹅
Excellent Performance and Ergonomics

The Midi Plus is a lightweight into a culture media dish can be

electronic cordless pipetting performed carefully, drop by drop,
controller, which allows aspiration without breaking the fine surface of
from bottles and tubes, without the the medium.
arm and hand elevations required in
the case of serological or volumetric ‒‒ Stepless speed control
pipettes. It fits all commonly used ‒‒ Hydrophobic autoclavable filter
1 - 100 ml glass or plastic pipettes, protects the device in case of
but can also be used with Sartorius over-aspiration
5 ml and 10 ml disposable tips. The ‒‒ Convenient fold-out bench stand Stepless speed control
speed can be fine-tuned by applying supports the unit and pipette when ‒‒ Allows the aspiration and
varying finger pressure to the not in use* dispensing speed to be adjusted
operating buttons. ‒‒ Low battery warning easily and precisely to suit either
large or small volume pipettes.
The Midi Plus is ideal, for example,
in microbiological work: dispensing
Fold-out stand*
‒‒ Prevents pipette contamination
‒‒ Saves space
‒‒ Allows the unit to be rested on a
table with a pipette attached

Hydrophobic autoclavable
filter prevents over-
aspiration

Autoclavable pipette
adapter set

Fits all commonly used

1-100 ml glass or plastic
pipettes

Operating buttons

*Not available in units sold in the United States

 Midi Plus Maxi-volume Liquid Handling 69

⹅⹅
Features
Pipette types Plastic or glass 1– 100 ml
Pasteur pipettes
5 ml and 10 ml Sartorius pipette tips
Rechargeable during use Yes
Speed control Stepless adjustable control
Gravity dispensing Yes
Stand Attached support
Weight 207 g
Low battery indicator Yes
Autoclavable parts Nose cone, pipette holder and filter

⹅⹅
Ordering Information
Order Code Item Qty/Unit
710931 Midi Plus Pipetting Controller with 1
Universal Charger1
LH-7129120 Replacement filter, 0,45 µm, non-sterile, autoclavable 5
LH-7129130 Replacement filter, 0,2 µm, sterile 5
LH-711019 Adapter set (standard), autoclavable, including 1
nose-cone and silicone adapter
LH-711017 Adapter set for 5 ml pipette tip, autoclavable, 1
including nose-cone and silicone adapter
LH-711018 Adapter set for 10 ml pipette tip, autoclavable, 1
including nose-cone and silicone adapter
780300 Optifit Tip 5 ml (length 150 mm) 100
780308 Optifit Tip 5 ml (length 150 mm) 1000
780310 Midi Plus Tip 10 ml (length 242 mm) 100

1) Supplied with a universal charger (incl. EU, UK, US | JPN, AUS, KOR and CHN plugs)
70 Maxi-volume Liquid Handling Proline® Prospenser

Proline® Prospenser
⹅⹅
Easy-to-use Bottle-top Dispenser

Proline® Prospenser has been designed for trouble-free and reliable bottle-top
dispensing of liquids, including concentrated acids, bases, saline solutions, as
well as many organic solvents.

‒‒ Dispensing directly from the supply ‒‒ Wide range of adapters included to

bottle fit the most common bottle sizes
‒‒ Fully autoclavable at 121˚C, 2 bar, ‒‒ Optional flexible dispense tube
20 minutes extension (max length 800 mm)
‒‒ Accuracy of delivery within ± 0.5% with safety handle enables fast
Volume setting easy
‒‒ Chemically resistant fluid pathway and precise dispensing even into
to adjust
‒‒ Anti-drip closure cap included narrow tubes
‒‒ Easy-to-use volume adjustment for ‒‒ Each unit is supplied with
reproducible dispensings performance certificate and tested
‒‒ Easy to dismantle for cleaning and according to ISO 8655
maintenance

Borosilicate glass
barrel protected
with a transparent
polypropylene sleeve

Anti-drip
closure cap

Wide range of
bottle adapters
included

Chemically
resistant liquid
pathway

Optional flexible dispense

tube extension
‒‒ Coiled
‒‒ Length 800 mm

Fully autoclavable
 Prospenser Maxi-volume Liquid Handling 71

Prospenser
⹅⹅
Bottle-top Dispenser with Anti-drip Valve

The Prospenser bottle-top dispenser delivers safe and precise liquid Easy-to-adjust
dispensing, including of strong acids, bases and solvents. Special calibration
features include an easy-to-adjust calibration mechanism and mechanism.
precision valve for enhanced accuracy and usability.

‒‒ Dispensing directly from the supply ‒‒ Wide range of adapters

bottle included to fit the most
‒‒ Easy-to-adjust calibration common bottle sizes
mechanism ‒‒ Optional dispense tube
‒‒ Fully autoclavable at 121˚C, 2 bar, extension allows fast and safe
20 minutes dispensing even into narrow
‒‒ Accuracy of delivery within ± 0.3% tubes
‒‒ Chemically resistant fluid pathway ‒‒ Manufactured to ISO9002
‒‒ Anti-drip precision valve standards, each unit is
mechanism ensures easy priming supplied with an individual Borosilicate glass
and minimum waste with no calibration certificate barrel protected
leakage back into the reservoir with a transparent
‒‒ Easy-to-use volume adjustment for polypropylene sleeve
reproducible dispensing
‒‒ Unlike other bottle-top dispensers, Easily removable PTFE
Prospenser’s glass barrel can be piston for cleaning and
disassembled from the pedestal for smooth action
thorough cleaning

Chemically resistant
liquid pathway

Precision valve
mechanism ensures that
the Prospenser stays fully
Bubble free primed all day
dispensing

Anti-drip
tap valve
Set of adaptors
available

Optional dispense tube extension

‒‒ Allows fast and safe dispensing
into narrow tubes

Fully autoclavable
72 Maxi-volume Liquid Handling Biotrate

Biotrate Digital Burettes

⹅⹅
Ranges of 0 – 30 ml and 0 – 50 ml

The streamlined Biotrate digital burette and dispenser

delivers accurate, precise and convenient bottle-top titration,
Rotating head and
as well as optimum operator safety. Due to its life-long
clear numbers on the
battery concept, there is no need for an electrical outlet.
display increase working
This feature makes Biotrate ideal for both laboratory and
reliability
field-based analyses, where it can be easily moved from one
place to another.

‒‒ Chemically resistant and autoclavable liquid-path

construction is made of borosilicate glass, PTFE, PVDF, FEP
and ceramic components Chemically resistant and
‒‒ Based on positive displacement principle for maximum autoclavable liquid pathway
accuracy
‒‒ An automatic low battery indication is shown on the large
easy-to-read LCD display
‒‒ Simple for user to calibrate and restore factory calibration
‒‒ With a zero reset feature, it is easy to move from one
titration to another

Simple user calibration

and factory reset
for ISO 9000 and GLP
requirements

Visible borosilicate glass

barrel protected with a
transparent polypropylene
sleeve allows inspection of
liquid before dispensing

Anti-drip tap

Bubble-free
dispensing

Fully autoclavable
 Ordering Information Maxi-volume Liquid Handling 73

⹅⹅
Ordering Information and Performance Specifications

Proline® Prospenser

Order Code Item Increment Max Systematic Random

Volume Error (%) Error (%)
723045 Proline® Prospenser 0.5 – 5 ml 0,1 ml 5 ml 0,5 0,1
(with 25, 28, 32, 38 and 40 mm adaptors)
723046 Proline® Prospenser 1 – 10 ml 0,2 ml 10 ml 0,5 0,1
(with 25, 28, 32, 38 and 40 mm adaptors)
723047 Proline® Prospenser 2.5 – 25 ml 0,5 ml 25 ml 0,5 0,1
(with 32, 38 and 40 mm adaptors)
723048 Proline® Prospenser 5 – 50 ml 1,0 ml 50 ml 0,5 0,1
(with 32, 38 and 40 mm adaptors)
721633 Flexible tube extension for 5 and 10 ml Proline® Prospensers
721634 Flexible tube extension for 25 and 50 ml Proline® Prospensers

Prospenser

Order Code Item Increment Max Systematic Random

Volume Error (%) Error (%)
723049 Prospenser 0.01 – 2.5 ml (with 38, 40 and 45 mm adaptors) 0,05 ml 2,5 ml 0,3 0,1
723050 Prospenser 0.1 – 5 ml (with 38, 40 and 45 mm adaptors) 0,1 ml 5 ml 0,3 0,1
723051 Prospenser 0.2 – 10 ml (with 38, 40 and 45 mm adaptors) 0,2 ml 10 ml 0,3 0,1
723052 Prospenser 1 – 30 ml (with 38, 40 and 45 mm adaptors) 1,0 ml 30 ml 0,3 0,1
723053 Prospenser 1 – 50 ml (with 38, 40 and 45mm adaptors) 1,0 ml 50 ml 0,3 0,1
721998 Dispense tube extension

Biotrate Digital Burettes 0 – 30 ml and 0 – 50 ml

Order Code Item Increment Systematic Random

Error (%) Error (%)
723054 Biotrate 0 – 30 ml (with 33, 38 and 45 mm adaptors) 0,01 ml 0,2 0,1
723055 Biotrate 0 – 50 ml (with 33, 38 and 45 mm adaptors) 0,01 ml 0,2 0,1
721998 Expandable delivery jet
 Table of Contents Pipetting Academy 75 75

Pipetting Academy
⹅⹅
Table of Contents

76 Pipetting Academy
78 Pipetting Recommendations
76 Pipetting Academy Pipetting Academy

Pipetting Academy
⹅⹅
Training for Better Performance, Ergonomics and Safety

Are you concerned about the results What Will You Learn?
Seminars include of your work due to poor pipetting ‒‒ Get hands-on training in pipetting
both theory and practices or RSI (Repetitive Strain techniques that will help you and
practice! Injury)? your co-workers to obtain more
accurate and precise results in the
Videos and Have you considered that the pain in lab
animations support your hand or arm may be related to ‒‒ Gain a better understanding of the
learning. the instruments or techniques you influence of pipetting techniques
use? and environmental factors on
testing results
Do you know which pipetting ‒‒ Be guided through the essentials of
technique to use with different types laboratory ergonomics
of liquids? ‒‒ Gain a better understanding of the
ergonomic risks in the laboratory
Do your results vary between users? environment and liquid handling in
particular
The Pipetting Academy seminar ‒‒ Learn how to avoid these risks by
offers a comprehensive tutorial choosing the most appropriate
package, developed to answer working postures, liquid handling
these questions together with you. devices and accessories
During the seminars, you will learn ‒‒ Appreciate how you can help make
to recognise pipetting-related risk savings in both direct and indirect
factors and increase your knowledge costs due to bad ergonomics
of ergonomics, safety and pipetting ‒‒ Become able to instruct your
techniques, in order to avoid these employees on all of these issues,
risks in your daily work. making work more efficient and
enjoyable.
 Pipetting Academy Pipetting Academy 77

⹅⹅
Build Your Own Seminar
Pipetting Academy offers you various ergonomically and efficiently. Pipette Service
seminars for different purposes. Understand the risks and learn about Learn which aspects to consider to
the solutions. keep your pipette in good working
You May Choose from condition to deliver accurate results
Pipetting Techniques repeatedly, year after year.
Ergonomics Master your working tool. Handle the
Learn about the optimal posture pipette correctly. Be guided through
for pipetting and become familiar the many techniques of which your
with tools that can help you work pipette is capable.

⹅⹅
How to Sign Up for Seminars?
‒‒ To sign up for the seminar, contact Gain access to educational material, through reverse pipetting, diluting
your local Sartorius representative videos and animations and all other pipetting modes, to
‒‒ The seminar will be held in the Once you have signed up and make your work easier and more
location most suitable to you and participated in the seminar, you efficient
your colleagues will automatically gain access to ‒‒ A Certified Professional Ergonomist
‒‒ The trainer will be certified to hold educational videos, animations will guide you through the
Pipetting Academy seminars and presentations on ergonomics, essentials of ergonomics
‒‒ Each participate will receive a pipetting techniques and calibration. ‒‒ Presentations with explanations
certificate of participation after and illustrations on calibration and
the seminar ‒‒ Through these animations, you quality standards, are also available
will be guided step by step in the
correct handling of the pipette and See You at the Pipetting Academy!
78 Pipetting Academy Pipetting Recommendations

Pipetting Recommendations
⹅⹅

Preparations Before Pipetting While Pipetting

‒‒ Use the tip specified by the ‒‒ Hold the pipette in a vertical
manufacturer. position. Tilting the pipette at an
‒‒ Ensure that the pipette and the angle causes a volume greater than
tip have been tested according the set volume of liquid to enter
to ISO 8655 and the tip is seated the tip.
correctly. ‒‒ In most cases, pre-rinsing of the
‒‒ Make sure pipettes have been tip is recommended, to achieve
correctly calibrated. accurate results. Do not pre-rinse
‒‒ Check that the pipette, tip the tip, if the temperature of the
and liquid are all at the same liquid is different to the ambient
temperature. temperature.
‒‒ When pipetting liquids with ‒‒ When aspirating fluid, the pipette
temperatures different to the tip should normally be immersed to
ambient temperature, do not pre- a depth of 2-3 mm.
Hold the pipette in a vertical rinse the tip. Change the tip after ‒‒ When using a mechanical pipette,
position during aspiration each pipetting. operate the piston with a smooth
‒‒ Ensure that any fluid viscosity and consistent thumb action, for
variations have been accounted repeated results without foaming
for and the correct technique is or bubbles.
employed, i.e. reverse pipetting. ‒‒ You should pipette against the
‒‒ If handling infectious or inside wall of the receiving vessel.
radioactive agents make sure Remove the tip by drawing it
appropriate shielding and other upwards against the inside wall.
precautions protect the operator. ‒‒ Ensure that the pipette blow out
‒‒ Use Safe-Cone Filter in the tip action is fully activated.
cone whenever possible. ‒‒ Ensure that the volume is still set
at the required position. A pipette
Avoid contamination with with a volume locking mechanism
Safe-Cone Filters is recommended, in order to avoid
accidental volume change during
pipetting.
‒‒ Avoid leaving the pipette on its
side
with liquid in the tip, which may
seep back into the mechanism.

mLINE® volume lock prevents

volume changes during
pipetting
 Pipetting Recommendations Pipetting Academy 79

Other Precautions ‒‒ Avoid exposing the unit to extreme

‒‒ Store the pipette on a stand temperature changes, humidity
when not in use – see pages 46- and
47, on pipette stands, for more dust (operating temperature from
information. Electronic pipettes 15°C to 40°C).
should be returned to their ‒‒ Service the pipette regularly.
charging stands. ‒‒ Clean the pipette thoroughly
‒‒ Avoid dropping the pipette or before sending it in for service.
allowing contact with dirt or Decontaminate the pipette with
Charging while pipetting is grease. 70% ethanol. Notify the service
possible with Sartorius ‒‒ Change the Safe-Cone Filter personnel of the purpose for
electronic pipettes regularly (recommendation after which the instrument has been
50 to 250 pipetting cycles), and in used. Postal services may refuse
every case of over-aspiration. to deliver instruments used for
‒‒ Never strike the tip cone against hazardous materials. Make sure
the tip tray when loading the tip, that a qualified person services the
as this can damage the pipette. pipette.

Load the tip onto the pipette

carefully and take advantage
of the Optiload tip loading
mechanism

Clean the pipette before

sending it to service
 Table of Contents Calibration & Maintenance Services 8181

Calibration and Maintenance

⹅⹅
Services
Table of Contents

82 Pipette Calibration and Maintenance Services

86 Pipette Decontamination Procedure
87 Autoclaving Instructions
87 Troubleshooting Guide
82 Calibration & Maintenance Services Service

Pipette Calibration and Maintenance Services

⹅⹅

Why is Calibration and Maintenance Sartorius Service Centres

Needed?
It is vitally important to Sartorius
All pipette manufacturers that our customers receive world
recommend that regular maintenance class service and support, from the
and calibration is applied to maintain first phone call to the moment the
reliable pipetting results and to engineer leaves, with the customer’s
maximize the lifetime of the pipette. equipment in perfect working
condition.
Pipette calibration is a fundamental
part of Good Laboratory Practice Sartorius provides a global network
(GLP) and quality systems and must of service centers for calibration of
be considered a vital part of any all makes and models of pipettes,
laboratory regime where precise burettes, bottle top dispensers and
volumes of fluid need transferring other liquid handling instruments.
or diluting. Pipette performance is With over 20 years of experience
measured as accuracy and precision in pipettes and liquid handling
or how close the dispensed volumes instrument services, the global
are to the target and how close the organization provides world class
results are to each other. services tailored to customers’ needs,
simultaneously fulfilling standards
and regulatory body requirements.
With the Sartorius concept of “all
makes and models liquid handling
services” you can be certain that your
instruments are handled according
to the international ISO 8655
standard defining the use and
calibration of pipettes and other
liquid handling instruments. Sartorius
provides reliability of operation,
reduced instrument downtime
and confidence, so that your
work is according to the strictest
requirements.
 Service Calibration & Maintenance Services 83

⹅⹅
Maintenance, Repair and Calibration Services

Maintenance consists of the cleaning ‒‒ Pipette calibration done according

and lubrication of the piston and to ISO 8655 standard by our
tip cone(s) of the pipette, and the network of ISO 17025 accredited
replacment of parts prone to wear, pipette calibration laboratories
in order to guarantee a long lifetime around the world
and consistently accurate and precise ‒‒ Pipette maintenance and
results. calibration services available
either as mail-in service at service
As with all mechanical devices, centers or as on-site service at the
pipettes may need repairs. It is customer’s laboratory
usually worth the expense to ‒‒ Repair services and spare parts for
maintain and repair the pipettes all makes and models of pipettes
rather than to dispose of them. This available
is also an ecologically sustainable ‒‒ Extended Warranty for an
choice. Sartorius provides spare parts additional 12 months
and repairs for any make and model.
If the pipettes are beyond economical Benefits
repair, then we offer replacements. ‒‒ Maximum reliability and lifetime
with regular maintenance and
Calibration and maintenance can be original spare parts
done either at a dedicated calibration ‒‒ Confidence in the quality of
laboratory, or at the customer site. your work provided by regular
Laboratory conditions can provide a calibration
smaller uncertainty of measurement ‒‒ All documentation needs met by
in calibration, but the quickest our detailed service and calibration
turnaround time is often achieved documentation, including
by performing maintenance and ISO 17025 accredited calibration
calibration on-site. certificates with measurement
results traceable to international
Services Provided standards
‒‒ Maintenance and calibration for all ‒‒ Efficient and time saving services
makes and models of pipettes and provided either at your site or as
other liquid handling instruments, a mail-in service, which includes
including cleaning, relubrication courier collection and delivery
and adjustment of instruments ‒‒ Affordable, planned warranty of
your equipment
84 Calibration & Maintenance Services Service

⹅⹅
Accreditation and Calibration Standards

Good Laboratory Practices (GLP), Recognition Agreements (MLA)

accredited processes and quality ensuring that accredited pipette
systems require traceability of calibration certificates are accepted
measurement and compliance with around the world.
a multitude of standards. Sartorius
operates a global network of Ask your local Sartorius
accredited calibration laboratories, representative for more information
providing services around the world. on accredited pipette calibration
services available for you.
What is ISO 17025?
The ISO 17025 standard specifies What is ISO 8655?
the general requirements for The ISO 8655 standard specifies the
the competence of calibration requirements for piston operated
laboratories to carry out tests and volumetric apparatus (pipettes) and
calibrations. A pipette calibration pipette calibration laboratories,
laboratory with an ISO 17025 providing detailed requirements
accreditation provides the highest for procedures and equipment
level of reliability and confidence in used in pipette calibration. Pipettes
pipette calibration, proven with a calibration, according to ISO 8655,
Measurement Uncertainty Certificate is done in a carefully controlled
provided with each calibration, when environment, with no drafts or
required. Gaining and maintaining vibrations, using repeatable and
an ISO 17025 accreditation requires reliable measurement technology.
extensive reviewing and development Sartorius balances meet such
of personnel, procedures and specifications and calibration is
facilities, audited annually by the always done according to the
accreditation body. strictest, repeatable procedures.

Sartorius provides ISO 17025 Making sure your pipettes are

accredited pipette calibration services calibrated according to ISO
in various countries, for example 8655, Sartorius provides the best
DAkkS in Germany, Cofrac in France, confidence in the reliability of results.
UKAS in the United Kingdom, A-Class Sartorius pipette calibration is always
in the USA, JCSS in Japan, and FINAS done according to ISO 8655.
in Finland. All European accreditation
bodies are members of the European
Co-operation for Accreditation (EA)
and signatories of the Multilateral
 Service Calibration & Maintenance Services 85

⹅⹅
In Conclusion
Adopting a regular calibration and maintenance routine for your pipette has
the following benefits:

Confidence
Your pipettes are operating correctly with the accuracy and precision you
know.

Reliability
With maintenance and calibration and operational qualification you are able
to trust the instrument’s operational capability.

Efficiency and Effectiveness

With properly working pipettes you can work uninterrupted and be more
efficient.

Sustainability
Extending the lifespan of your pipette reduces waste, and consequently is an
environmentally considerate choice.

⹅⹅
Frequently Asked Questions
Question: What makes and models laboratory site. Ask for on-site
of pipettes does Sartorius service? services, especially if you need your
pipettes every day.
Answer: Sartorius provides
calibration and maintenance services Question: Why should I get a
for all manufacturers’ pipettes, Service Contract?
the most common being Sartorius
(Biohitfamily), Gilson, Eppendorf, Answer: Often the most economical
and Rainin. With over 20 years of and easiest way to handle your
experience in the field we have the calibration and maintenance needs,
skill and ability to service any pipette. is to enter into a contract with a
service provider. This saves time,
Question: What is pipette and is usually the most economical
calibration? choice. Sartorius service technicians
are experts in pipettes and also offer
Answer: It is when a pipette is training in the use of instruments.
tested for inaccuracy, imprecision They can also offer replacements for
and linearity to determine the worn out pipettes.
uncertainties of measurement
for each volume calibrated. Test Question: How often should my
volumes and data points are pipettes be calibrated?
selected according to the customer
requirements. Answer: The ISO 8655 standard
states that pipettes should be
Question: Can I have my pipettes calibrated regularly, at least annually,
calibrated in my laboratory, as I or more often, for example every 3-6
need them every day? months. It depends on the accuracy
requirements of your work as well as
Answer: Yes. Sartorius offers the nature of liquids used - corrosive
calibration and maintenance services or volatile liquids create more wear
both at our workshops and at your and tear on your pipettes.
86 Calibration & Maintenance Services Pipette Decontamination Procedure

Pipette Decontamination Procedure

⹅⹅

Mechanical Pipettes (mLINE® and Proline® Plus) Electronic Pipettes (Picus®, Picus® NxT and eLINE®)

1. Unscrew the tip ejector collar counter clockwise and remove it. 1. Unscrew the tip ejector collar counter clockwise and remove it.

2. Unscrew the tip cone holder counter clockwise and carefully 2. Unscrew the tip cone holder counter clockwise and carefully
remove it along with the tip cone. Remove the Safe-Cone Filter remove the tip cone holder, tip cone and spring.Remove the
if fitted. Safe-Cone Filter if fitted.

3. Unscrew the piston counter clockwise from the pipette. 3. Unscrew the piston counter clockwise from the pipette.

4. Place the tip ejector collar, tip cone holder, tip cone and tip 4. Place the tip cone, tip cone holder, tip ejector collar, piston and
cone cylinder into a beaker containing 70% ethanol and leave spring into a beaker containing 70% ethanol and leave for at
for at least 30 minutes. least 30 minutes.
5. After performing the procedure described above, remove the 5. After performing the procedure described above, remove the
components from the beaker and rinse them with distilled components from the beaker and rinse them with distilled
water, then dry preferably with warm air, for at least an hour. water, then dry preferably in warm air, for at least an hour.
6. Re-grease the piston as described in the instruction manual. 6. Re-grease the piston as described in the instruction manual.
Replace all components including new filter if fitted. Replace all components including the new filter if fitted.

Note: When performing the decontamination procedure, as a matter of routine the o-ring seal should be checked for possible wear on
every sixth occasion, and replaced if necessary. Calibration should also be checked at the same time.
 Autoclaving Instructions Calibration & Maintenance Services 87

Autoclaving Instructions
⹅⹅

mLINE® and Proline® Plus as individual parts. It is also possible

Mechanical Pipettes to clean the parts and grease the
The entire mLINE® and Proline® piston prior to autoclaving.
Fully autoclavable
Plus mechanical pipette can be ‒‒ Remove the Safe-Cone Filter
steam sterilized by autoclaving at (if fitted).
121°C (252°F), 1 bar (15 p.s.i.) for ‒‒ Put the dispensing head into the
20 minutes. The dispensing head of sterilisation bag and place it into
the multi-channel pipettes must be the autoclave
Autoclavable
lower parts unscrewed 360° counter clockwise ‒‒ After autoclaving the parts must
before autoclaving. be cooled down and left to dry
before use
‒‒ Remove the Safe-Cone Filter
(if fitted) It is recommended that you check
‒‒ Put the pipette into the the performance of the pipette after
Autoclavable liquid
pathway sterilisation bag and place it into every autoclaving, and grease the
the autoclave piston | seal of the pipette after every
‒‒ After autoclaving the pipette must 10th autoclaving.
be cooled down and left to dry
overnight before use Tips and Tip Boxes
‒‒ Place the bulk tips into the
It is recommended that you check sterilisation bag and the tip tray as
the performance of the pipette after such in the autoclave
every autoclaving, and grease the ‒‒ Autoclave for 20 minutes at a
piston | seal of the pipette after every temperature of 121°C under 1 bar
10th autoclaving. (15 p.s.i.)
‒‒ Cool before use
Picus®, Picus® NxT and eLINE®
Electronic Pipettes Lower Parts Note:
The dispensing head (tip ejector ‒‒ Excessive heat or length of time
collar, tip cone holder, tip cone, may damage the products. Never
spring and piston) of the single- place the handle part of the Picus®,
channel and multi-channel models Picus® NxT or eLINE® into the
(except for multi-channel 1200 µl) autoclave
can be autoclaved (121°C, 1 bar for ‒‒ The lower ends of multi-channel
20 minutes). These parts can be pipettes are not interchangeable
autoclaved as one unit or separately between 8 and 12-channel pipettes
‒‒ The cover of the tip tray should be
closed during autoclaving
88 Calibration & Maintenance Services Troubleshooting Guide

Troubleshooting Guide
⹅⹅

Problem Cause Solution

Droplets left inside the tip Unsuitable tip Use original Sartorius tips
Non-uniform wetting of the tip plastic Attach new tip and pre-wet it
Optiload not fully utilised and thus tip Pick up the tip so that it strikes the lower
does not fit very well (All mLINE® edge of the tip ejector collar
models and Proline® Plus MC-models
and 2ml, 5ml and 10ml SC-models have
Optiload)
Leakage or pipetted volume Tip incorrectly attached Attach firmly
too small Unsuitable tip Use original Sartorius tips
Tip is leaking and | or Optiload not Replace a new tip or see above for Optiload
fully utilized
Plunger movement not uniform, It is very important that the plunger
constant or balanced movement is slow and that this is always done
in the same way during pipetting cycles.

Volumes 1ml and greater: if plunger is

released too quickly upon aspirating, it will
affect the amount of liquid drawn.
Test results are incorrect Improper maintenance of lower parts Clean and re-grease lower parts as per
and/or results are non-linear instructions in the pipette’s manual, only
applies to single-channel pipettes
Piston or tip cone damaged/non-linear Replace part with new one, only applies to
single-channel pipettes
Dirt on stop surfaces/inside the Clean the stop surfaces/interior of the
machinery machinery as per instructions
Uneven piston movement |inconsistent It is very important that the plunger
pipetting technique/rhythm movement is slow and that this is always done
in the same way during pipetting cycles.

Volumes 1ml and greater: if the plunger is

released too quickly upon aspirating, it will
affect the amount of liquid drawn.
Display not sitting properly Calibration wheel (726066) not properly Remove display and push calibration wheel
(in particular the lower edge in place down
of the display does not fit)
Counter reading does not The wings on the lead screw have come Return the wings to the grooves
make sense and /or counter out of the grooves on the machinery
feels very slack The wings on the lead screw have been Replace machinery with new parts
broken
There is something broken inside the Replace machinery with new parts
machinery
Multi-channel pipettes: Tip cone (bajonet connection) has Refit tip cone
tip cone(s) does not accidentally come off
draw liquid Piston(s) is not connected to the piston Refit piston(s) so that they connect to the
support plate piston support plate properly
Piston/tip cone damaged Replace parts with new ones
Improper maintenance Maintain lower parts as per instructions
 Troubleshooting Guide Calibration & Maintenance Services 89

Problem Cause Solution

Pipette does not draw liquid Magnet holder/magnet is disconnected Remove the dispensing head and refit it so
at all from the lower part that the magnet holder/magnet is properly
connected to the dispensing head
Multi-channel pipettes: Tip ejection bar has come off Make sure that the tip ejection bar is
Tip ejection does not work/ (the snap fit is disengaged) properly connected
tip does not fit
Electronic pipettes: Error inDischarged battery/defective battery Recharge battery/replace battery
the display and motor is Actuator rod jammed Clean and lubricate actuator rod
unable to start Penetration of solvent vapours and thus Clean tip ejection mechanism and clean/
actuator rod/tip ejection mechanism lubricate actuator rod
jammed
Failure in the handle parts (upper body Check error messages
parts)
Electronic pipette can start Discharged battery/defective battery Recharge battery/replace battery
but is unable to complete Tip ejector mechanism jammed/ Clean tip ejection mechanism and
self test when switched on contaminated clean/lubricate actuator rod
(error blinking on the Failure in the handle parts Check error messages
display) (upper body parts)
Internal error has occurred Check error messages and proceed accoringly
Tip ejector jammed or moves Ejector mechanism contaminated Clean lower parts of pipette
erratically Ejector mechanism damaged Replace damaged parts
Tip ejector feels slack Ejector mechanism damaged Replace damaged parts
Fading display and/or Display damaged or incorrectly attached Open handle cover and check the display
segment missing to the PCU-board/defective PCU board
Autotest (a test programmed Discharged battery/defective battery Replace a battery
by service engineer) failed Failure in the handle parts Check error counters and autotest counters
(upper body parts) and proceed accordingly
Pipette not charging Pipette incorrectly positioned in the Check the position of the pipette
charging stand/carousel
AC-adaptor damaged Replace AC-adaptor
Charging carousel/stand damaged Open the bottom, check wires and replace
the unit if needed
Battery contacts inside the battery Clean battery contacts
compartment damaged/oxidated/
flattened
Battery damaged Replace battery
Reduced operating time with Batteries damaged Replace battery
fully charged batteries
90 Sales and Service Contacts Europe

Sales and Service Contacts

⹅⹅
For further contacts, visit www.sartorius.com

⹅⹅
Europe
Germany Hungary Russian Federation
Sartorius Lab Instruments Sartorius Hungária Kft. LLC “Sartorius ICR” and LLC “Biohit”
GmbH & Co. KG Kagyló u. 5. Uralskaya str. 4, Lit. B
Weender Landstrasse 94–108 2092 Budakeszi 199155, Saint-Petersburg
37075 Goettingen Phone +3623.457.227 Phone +7.812.327.5.327
Phone +49.551.308.0 Fax +3623.457.147 Fax +7.812.327.5.323
Fax +49.551.308.3289
Ireland Spain & Portugal
France & Suisse Romande Sartorius Ireland Ltd. Sartorius Spain, S.A.
Sartorius France Unit 41, The Business Cen-tre Avda. de la Industria, 32
2, rue Antoine Laurent de Lavoisier Stadium Business Park Edificio PAYMA
ZA de la Gaudrée Ballycoolin Road 28108 Alcobendas (Madrid)
91410 Dourdan Dublin 11 Phone Spain +34.902.123.367
Phone +33.1.70.62.50.00 Phone +353.1.8089050 Phone Portugal +351.800.855.800
Fax +33.1.64.59.76.39 Fax +353.1.8089388 Fax Spain +34.91.358.96.23
Fax Portugal +351.800.855.799
Austria Italy
Sartorius Austria GmbH Sartorius Italy S.r.l. Switzerland
Franzosengraben 12 Viale A. Casati, 4 Sartorius Mechatronics Switzerland AG
1030 Vienna 20835 Muggiò (MB) Ringstrasse 24a
Phone +43.1.7965760.0 Phone +39.039.4659.1 8317 Tagelswangen (ZH)
Fax +43.1.7965760.24 Fax +39.039.4659.88 Phone +41.44.746.50.00
Fax +41.44.746.50.50
Belgium Netherlands
Sartorius Belgium N.V. Sartorius Netherlands B.V. U.K.
Leuvensesteenweg, 248/B Phone +31.30.60.53.001 Sartorius UK Ltd.
1800 Vilvoorde Fax +31.30.60.52.917 Longmead Business Centre
Phone +32.2.756.06.71 [email protected] Blenheim Road, Epsom
Fax +32.2.253.45.95 Surrey KT19 9QQ
Poland Phone +44.1372.737159
Finland & Baltics Sartorius Poland sp.z o.o. Fax +44.1372.726171
Sartorius Biohit Liquid Handling Oy ul. Wrzesinska 70
Laippatie 1 62-025 Kostrzyn Ukraine
00880 Helsinki Phone +48.61.6473830 LLC “Biohit”
Phone +358.9.755.951 Fax +48.61.6473839 Post Box 440 “B”
Fax +358.9.755.95.200 01001 Kiev, Ukraine
Phone +380.44.411.4918
Fax +380.50.623.3162
 America and Asia Pacific Sales and Service Contacts 91

⹅⹅
America ⹅⹅
Asia | Pacific
USA Australia Malaysia
Sartorius Corporation Sartorius Australia Pty. Ltd. Sartorius Malaysia Sdn. Bhd
5 Orville Drive, Suite 200 Unit 5, 7-11 Rodeo Drive Lot L3-E-3B, Enterprise 4
Bohemia, NY 11716 Dandenong South Vic 3175 Technology Park Malaysia
Phone +1.631.254.4249 Phone +61.3.8762.1800 Bukit Jalil
Toll-free +1.800.635.2906 Fax +61.3.8762.1828 57000 Kuala Lumpur, Malay-sia
Fax +1.631.254.4253 Phone +60.3.8996.0622
China Fax +60.3.8996.0755
Argentina Sartorius Scientific
Sartorius Argentina S.A. Instruments (Beijing) Co., Ltd. Singapore
Int. A. Ávalos 4251 33 Yu An Road, Airport Industrial Park Sartorius Singapore Pte. Ltd
B1605ECS Munro Zone B, 1 Science Park Road,
Buenos Aires Shunyi District, Beijing 101300, The Capricorn, #05-08A,
Phone +54.11.4721.0505 P.R.China Singapore Science Park II
Fax +54.11.4762.2333 Phone +86.10.8042.6300 Singapore 117528
Fax +86.10.8042.6486 Phone +65.6872.3966
Brazil Fax +65.6778.2494
Sartorius do Brasil Ltda Hong Kong
Avenida Senador Vergueiro 2962 Sartorius Hong Kong Ltd. South Korea
São Bernardo do Campo Unit 1012, Lu Plaza Sartorius Korea Ltd.
CEP 09600-000 - SP- Brasil 2 Wing Yip Street 8th Floor, Solid Space B/D,
Phone +55.11.4362.8900 Kwun Tong PanGyoYeok-Ro 220, Bun-Dang-Gu
Fax + 55.11.4362.8901 Kowloon, Hong Kong SeongNam-Si, GyeongGi-Do, 463-400
Phone +852.2774.2678 Phone +82.31.622.5700
Canada Fax +852.2766.3526 Fax +82.31.622.5799
Sartorius Canada Inc.
2179 Dunwin Drive #4 India Thailand
Mississauga, ON L5L 1X2 Sartorius Weighing India Pvt. Ltd. Sartorius (Thailand) Co. Ltd.
Phone +1.905.569.7977 #69/2-69/3, NH 48, Jak-kasandra, 129 Rama 9 Road,
Toll-Free +1.800.668.4234 Nelamangala Tq Huaykwang
Fax +1.905.569.7021 562 123 Bangalore, India Bangkok 10310
Phone +91.80.4350.5250 Phone +66.2643.8361-6
Mexico Fax +91.80.4350.5253 Fax +66.2643.8367
Sartorius de México S.A. de C.V.
Circuito Circunvalación Poniente Japan
No. 149 Sartorius Japan K.K.
Ciudad Satélite 4th Fl., Daiwa Shinagawa North Bldg.
53100, Estado de México 8-11, Kita-Shinagawa 1-chome
México Shinagawa-ku, Tokyo, 140-0001 Japan
Phone +52.5555.62.1102 Phone +81.3.3740.5408
Fax +52.5555.62.2942 Fax +81.3.3740.5406
92 Notes

Notes
⹅⹅
 Notes 93

Notes
⹅⹅
94 Notes

Notes
⹅⹅
Did You know …
… that you can order more lab catalogs?
Get an overview of our high-quality laboratory instruments, high-grade
consumables and excellent services.
Our product portfolio focuses on laboratory instruments, such as lab balances,
pipettes and laboratory water purification systems. Moreover, we offer
the widest range of consumables like laboratory filters and pipette tips.

Products for
Microbiological Control Laboratory Weighing Products Sartorius Liquid
Laboratory Handling
Weighing Products
Products

Products for Laboratory Weighing Sartorius Liquid Handling

Microbiological Control Products Products
Publication No.: SL-1530-e Publication No.: WL-0007-e Publication No.: SUL0002-e
Order No.: 85034-538-81 Order No.: 98649-018-13 Order No.: 85032-542-25

Laboratory Water
Laboratory Filtration Products Purification Products

Laboratory Filtration Laboratory Water

Products Purification Products
Publication No.: SLU0006-e Publication No.: SL-0002-e
Order No.: 85032-543-83 Order No.: 85037-548-54

Download our lab catalogs from our website:

www.sartorius.com/lab-catalogs
Contact Details
Sartorius Biohit Liquid Handling Oy
Laippatie 1
00880 Helsinki, Finland
Phone +358.9.755.951
E-mail: [email protected]

Sartorius Lab Instruments GmbH & Co. KG

Weender Landstrasse 94-108
37075 Goettingen, Germany
Phone +49.551.308.0
Fax +49.551.308.3289
www.sartorius.com

The status of the information, specifications and

illustrations in this catalogue is indicated by the date
given below.

Sartorius reserves the right to make changes to the

technology, features, specifications and design of the
equipment without notice.

All trademarks are Sartorius property unless otherwise

stated. Patents granted or pending.

Publication No. SUL0002-e150806 ∙ Order No. 85032-542-25 ∙ Ver. 08 | 2015© Sartorius Biohit Liquid Handling Oy

 www.sartorius.com