CHAPTER 3.1.5.

CRIMEAN–CONGO HAEMORRHAGIC FEVER

SUMMARY
Crimean–Congo haemorrhagic fever virus (CCHFV) of the genus Orthonairovirus of the family
Nairoviridae causes a zoonotic disease in many countries of Asia, Africa, the Middle East and south-
eastern Europe. As the distribution of CCHFV coincides with the distribution of its main vector, ticks
of the genus Hyalomma, the spread of infected ticks into new, unaffected areas facilitates the spread
of the virus. The virus circulates in a tick–vertebrate–tick cycle, but can also be transmitted
horizontally and vertically within the tick population. Hyalomma ticks infest a wide spectrum of
different wildlife species, e.g. deer and hares, and free-ranging livestock animals, e.g. goat, cattle, and
sheep. Many birds are resistant to infection, but ostriches appear to be more susceptible. Viraemia in
livestock is short-lived, and of low intensity. These animals play a crucial role in the life cycle of ticks,
and in the transmission and amplification of the virus and are, therefore, in the focus of veterinary
public health. As animals do not develop clinical signs, CCHFV infections have no effect on the
economic burden regarding livestock animal production. In contrast to animals, infections of humans
can result in the development of a severe disease, Crimean–Congo haemorrhagic fever (CCHF).
Every year, more than 1000 human CCHF cases are reported with case fatality rates of 5–80%
depending on the virus strain and other local factors. The pathogenesis of the disease in humans is
not well understood. Most people become infected by tick bites and by crushing infected ticks, but
infection is also possible through contact with blood and other body fluids of viraemic animals, for
example in slaughterhouses. As CCHFV also has the potential to be transmitted directly from human-
to-human, nosocomial outbreaks have been reported.
There is no approved CCHF vaccine available and therapy is restricted to treatment of the symptoms.
Health education and information on prevention and behavioural measures are most important in
order to enhance public risk perception and, therefore, decrease the probability of infections. Thus
the identification of endemic areas is crucial for focused and targeted implementation of public health
measures. Serological screening of ruminants allows CCHFV-affected areas to be identified, as
antibody prevalence in animals is a good indicator of local virus circulation. Treatment with tick
repellents can be quite effective in reducing the tick infestation of animals. To protect laboratory staff,
handling of CCHFV infectious materials should only be carried out at an appropriate biocontainment
level.
Detection and identification of agent: Only a single virus serotype is known to date although
sequencing analysis indicates considerable genetic diversity. CCHFV has morphological and
physiochemical properties typical of the family Nairoviridae. The virus has a single-stranded,
negative-sense RNA genome consisting of three segments: L (large), M (medium) and S (small), each
of which is contained in a separate nucleocapsid within the virion. The virus can be isolated from
serum or plasma samples collected during the febrile or viraemic stage of infection, or from liver of
infected animals. Primary isolations are made by inoculation of several tissue cultures, commonly
African green monkey kidney (Vero) cells. For identification and characterisation of the virus,
conventional and real-time reverse transcription polymerase chain reaction (PCR) can be used. As
infections of animals remain clinically unapparent, the likelihood of isolating virus from a viraemic
animal is very low.
Serological tests: Type-specific antibodies are demonstrable by indirect immunofluorescence test
or by IgG-sandwich and IgM-capture enzyme-linked immunosorbent assay. Commercial test
systems are available for animal health; in addition a few in-house systems have been published or
kits are used replacing the conjugate provided in kit with one that is suitable for the animal species to
be screened for CCHFV-specific antibodies.
Requirements for vaccines: There is no vaccine available for animals.

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 Chapter 3.1.5. – Crimean–Congo haemorrhagic fever

A. INTRODUCTION

Crimean-Congo haemorrhagic fever (CCHF) is a zoonotic disease caused by a primarily tick-borne CCHF virus
(CCHFV) of the genus Orthonairovirus of the family Nairoviridae, order Bunyavirales. CCHFV possesses a negative-
sense RNA genome consisting of three segments, L (large), M (medium) and S (small) each contained in a separate
nucleocapsid within the virion. All orthonairoviruses are believed to be transmitted by either ixodid or argasid ticks,
and only three are known to be pathogenic to humans, namely CCHF, Dugbe and Nairobi sheep disease viruses
(Swanepoel & Burt, 2004; Swanepoel & Paweska, 2011; Whitehouse, 2004). CCHFV can be grown in several tick cell
lines derived from both a natural vector (Hyalomma anatolicum) and other tick species not implicated in natural
transmission of the virus (Bell-Sakyiet al., 2012).

The virus from an outbreak of “Crimean haemorrhagic fever” in the Crimean Peninsula in 1944 was not isolated or
characterised until 1967. “Congo haemorrhagic fever” virus, isolated from a patient in the former Zaire (now
Democratic Republic of the Congo) in 1956, was shown in 1969 to be the same virus. As a consequence the names
of both countries have been used in combination to describe the disease (Hoogstraal, 1979). Distribution of the virus
reflects the broad distribution of Hyalomma ticks, the predominant vector of the virus (Avsic-Zupanc, 2007; Grard
et al., 2011; Papa et al., 2011; Swanepoel & Paweska, 2011).

The natural cycle of CCHFV includes transovarial and transstadial transmission among ticks and a tick-vertebrate-
tick cycle involving a variety of wild and domestic animals. Infection can also be transferred between infected and
uninfected ticks during co-feeding on a host; so called ‘non-viraemic transmission’ phenomenon. Hyalomma ticks
feed on a variety of domestic ruminants (sheep, goats, and cattle), and wild herbivores, hares, hedgehogs, and
certain rodents. CCHFV infection in animals was reviewed by Nalca & Whitehouse (2007). Experimental infections
of wild animals and livestock with CCHFV were reviewed by Spengler et al. (2016). Although animal infections are
generally subclinical, the associated viraemia levels are sufficient to enable virus transmission to uninfected ticks
(Swanepoel & Burt, 2004; Swanepoel & Paweska, 2011). Many birds are resistant to infection, but ostriches appear
to be more susceptible than other bird species (Swanepoel et al., 1998). Although they do not appear to become
viraemic, ground feeding birds may act as a vehicle for spread of CCHFV infected ticks. Results from serological
surveys conducted in Africa and Eurasia indicate extensive circulation of the virus in livestock and wild vertebrates
(Swanepoel & Burt, 2004).

Humans acquire infection from tick bites, or from contact with infected blood or tissues from livestock or human
patients. After incubation humans can develop a severe disease with a prehaemorrhagic phase, a haemorrhagic
phase, and a convalescence period. Haemorrhagic manifestations can range from petechiae to large haematomas.
Bleeding can be observed in the nose, gastrointestinal system, uterus and urinary tract, and the respiratory tract,
with a case fatality rate ranging from 5% to 80% (Ergonul, 2006; Yen et al., 1985; Yilmaz et al., 2008).The severity of
CCHF in humans highlights the impact of this zoonotic disease on public health. Although CCHFV has no economic
impact on livestock animal production, the serological screening of animal serum samples for CCHFV-specific
antibodies is very important. As seroprevalence in animals is a good indicator for local virus circulation, such
investigations allow identification of high-risk areas for human infection (Mertens et al., 2013). Slaughterhouse
workers, veterinarians, stockmen and others involved with the livestock industry should be made aware of the
disease. They should take practical steps to limit or avoid exposure of naked skin to fresh blood and other animal
tissues, and to avoid tick bites and handling ticks. Experiences from South Africa demonstrated that the use of
repellents on animals before slaughter could reduce the numbers of infected slaughterhouse workers (Swanepoel
et al., 1998). The treatment of livestock in general can reduce the tick density among these animals and thus reduce
the risk of tick bite in animal handlers (Mertens et al., 2013). Such tick control by the use of acaricides is possible to
some extent, but may be difficult to implement under extensive farming conditions. Inactivated mouse brain
vaccine for the prevention of human infection has been used on a limited scale in Eastern Europe and the former
USSR (Swanepoel & Paweska, 2011). Progress in CCHFV vaccine development is being made with several different
approaches trialled to overcome current challenges (Dowall et al., 2017).

Infectivity of CCHFV is destroyed by boiling or autoclaving and low concentrations of formalin or beta-
propriolactone. The virus is sensitive to lipid solvents. It is labile in infected tissues after death, presumably due to
a fall in pH, but infectivity is retained for a few days at ambient temperature in serum, and for up to 3 weeks at 4°C.
Infectivity is stable at temperatures below –60°C (Swanepoel & Paweska, 2011). CCHFV should be handled with
appropriate biocontainment measures determined by risk analysis as described in Chapter 1.1.4 Biosafety and
biosecurity: Standard for managing biological risk in the veterinary laboratory and animal facilities (Palmer, 2011;
Whitehouse, 2004).

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 Chapter 3.1.5. – Crimean–Congo haemorrhagic fever

B. DIAGNOSTIC TECHNIQUES

Table 1. Diagnostic test formats for Crimean-Congo haemorrhagic fever virus infections in animals

Purpose

Population Individual animal Contribute Confirmation Immune status in

Method Prevalence of
freedom freedom from to of clinical individual animals or
infection –
from infection prior to eradication cases in populations post-
surveillance
infection movement policies animals vaccination

Detection and identification of the agent(a)

Real-time
– +++ – +++(c) +(b) –
RT-PCR

Virus isolation in
– – – +(c) – –
cell culture

Detection of immune response

IgG ELISA +++ + – + +++ –

Competitive
+++ + – + +++ –
ELISA

IgM ELISA – ++ – ++ – –

Key: +++ = recommended for this purpose; ++ recommended but has limitations;
+ = suitable in very limited circumstances; – = not appropriate for this purpose.
RT-PCR = reverse-transcription polymerase chain reaction; ELISA = enzyme-linked immunosorbent assay.
(a)
A combination of agent identification methods applied on the same clinical sample is recommended.
(b)
RT-PCR is used for the screening of tick populations in the context of surveillance studies.
(c)
Molecular testing/isolation can be used to confirm acute infection in rare cases in animals showing
clinical signs as viraemia tends to be transient.

CCHFV infection causes only a mild fever in domestic and wild vertebrate animals with a detectable viraemia of up
to 2 weeks (Gonzalez et al., 1998; Gunes et al., 2011). Similarly infected ostriches develop only low and short-lived
viraemia and no clinical signs (Swanepoel & Burt, 2004). Therefore, recent infections in animals are rarely
diagnosed and methods such as polymerase chain reaction (PCR), virus isolation in cell culture and IgM detection
by enzyme-linked immunosorbent assay (ELISA) are mainly used in human CCHF diagnostics or in the special case
that an animal has to be classified as CCHFV free. For prevalence analysis and for determination of whether CCHFV
is circulating in a country, methods for the detection of IgG antibodies are preferred (Table 1). If there is any
possibility or suspicion that diagnostic samples could be contaminated with CCHFV, they should be handled under
an adequate biosafety level and all persons dealing with those samples should be aware of the possible risk and
should use personal protective equipment to avoid human infections.

1. Detection and identification of the agent

For testing animals for viraemia, rapid diagnosis can be achieved by detection of viral nucleic acid in serum or
plasma using conventional (Burt et al., 1998) or real-time reverse transcription (RT-) PCR (Drosten et al., 2002; Duh
et al., 2006; Koehler et al., 2018; Negredo et al., 2017; Sas et al., 2018; Wolfel et al., 2007), or by demonstration of viral
antigen (Shepherd et al., 1988). Specimens to be submitted for laboratory confirmation of CCHF include blood and
liver samples. Because of the risk of laboratory-acquired infections, work with CCHFV should be conducted in
appropriate biosafety facilities.

The virus can be isolated from serum and organ suspensions in a wide variety of cell cultures, including Vero, LLC-
MK2, SW-13, BSR-T7/5, CER and BHK21 cells, and identified by immunofluorescence using specific antibodies.
Isolation and identification of virus can be achieved in 1–5 days, but cell cultures lack sensitivity and usually only
detect high concentrations of virus present in the blood.

1.1. Virus isolation in cell culture

CCHFV can be isolated in mammalian cell cultures. Vero cells are commonly used, usually yielding an
isolate between 1 and 5 days post-inoculation (p.i). CCHFV is poorly cytopathic and thus infectivity is

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 Chapter 3.1.5. – Crimean–Congo haemorrhagic fever

titrated by demonstration of immunofluorescence in infected cells (Shepherd et al., 1986). SW-13 cell line
has also been used extensively for virus isolation, producing plaques within 4 days (p.i.). Identification of
a CCHFV isolate has to be confirmed by immunofluorescence or molecular techniques (Burt et al., 1998;
Shepherd et al., 1986).

1.1.1. Test procedure

i) Susceptible cell lines include Vero-E6, BHK-21, LLC-MK2 and SW-13 cells. Inoculate 80%
confluent monolayers of the preferred cell line with the specimen. The volume of specimen
to be used depends on the size of the culture vessel (i.e. 25 cm2 culture flask or 6- or 24-well
tissue culture plate). The specimen volume should be sufficient to cover the cell monolayer.
Samples of insufficient volumes can be diluted with tissue culture medium to prepare
sufficient inoculation volume.
ii) Adsorb the specimen for 1 hour at 37°C.
iii) Remove inoculum. Add fresh tissue culture medium containing 2% fetal calf serum and
other required additives, as per specific medium and cell line requirements.
iv) Incubate at 37°C and 5% CO2 for 4–7 days.
v) Test supernatant for presence of CCHFV viral RNA using real-time RT-PCR as described
below, or perform immunofluorescence assay on cell scrapings.
vi) Isolates of CCHFV from clinical specimens cause no microscopically recognisable
cytopathic effects (CPE) in most of these cell lines.

1.2. Nucleic acid detection

Molecular-based diagnostic assays, such as RT-PCR, serve as the front-line tool in the diagnosis of CCHF,
as well as other viral haemorrhagic fevers (Drosten et al., 2003). The benefit of molecular diagnostic assays
is their rapidity compared to virus culture, often allowing a presumptive diagnosis to be reported within a
few hours after receiving a specimen (Burt et al., 1998). The RT-PCR is a sensitive method for diagnosis, but
because of the genetic diversity of CCHFV, there might be some challenges with regard to design of
primers or probes that allow detection of all circulating strains of the virus. Indeed, based on geographical
origin and phylogenetic analyses of the S gene segment, CCHFV has previously been classified into nine
geographical clades – four predominantly diffused in Africa, three in Europe, and two in Asia. Several real-
time RT-PCR assays that detect strains from different geographical locations have been evaluated (Gruber
et al., 2019). While some assays have been shown to be highly sensitive, detecting as little as 10 viral RNA
copies per ml of plasma, it is necessary to combine at least two molecular assays to ensure detection of
the different CCHFV clades (Gruber et al., 2019). The best assay combination(s) with the best detection
efficacy for each CCHFV clade, on the basis of all CCHFV sequences known at the time of the study, are
shown in Table 2. In addition, a low-density macroarray has been extensively validated in clinical specimens
collected from confirmed cases of CCHF over 20 years by a WHO reference laboratory. It was shown to
detect as few as 6.3 genome copies per reaction (Wolfel et al., 2009).

Table 2. Molecular assay combinations for the detection of CCHFV-specific nucleic acid

Clade Molecular assay combinations Primer and probe names (5’ → 3’ sequence)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Africa 1 Real-time RT-PCR Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Real-time RT-PCR
Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)
Africa 2
Fwd CCHF-SF2 (GGA-VTG-GTG-VAG-GGA-RTT-TG)
Real-time RT-PCR
Rev CCHF-SR2 (CAD-GGT-GGR-TTG-AAR-GC)
Fwd CCHF-N2 (CAA-RGG-CAA-RTA-CAT-MAT)

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 Chapter 3.1.5. – Crimean–Congo haemorrhagic fever

Clade Molecular assay combinations Primer and probe names (5’ → 3’ sequence)

Fwd CCHF1 (CTG-CTC-TGG-TGG-AGG-CAA-CAA)

Rev CCHF2_5 (TGG-GTT-GAA-GGC-CAT-GAT-GTA-T)
Nested Fwd CCHFn15 (AGG-TTT-CCG-TGT-CAA-TGC-AAA)
Nested RT-PCR
Nested Rev CCHFn25 (TTG-ACA-AAC-TCC-CTG-CAC-CAG-T)
Africa 3
Fwd CrCon1+ (RWA-AYG-GRC-TTR-TGG-AYA-CYT-TCA-C)
Nested RT-PCR
Rev CrCon1– (TRG-CAA-GRC-CKG-TWG-CRA-CWA-GWG-C)
Nested Fwd CriCon2+ (ART-GGA-GRA-ARG-AYA-TWG-GYT-TYC-G)
Nested Rev CriCon2– (CYT-TGA-YRA-AYT-CYC-TRC-ACC-ABT-C)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Real-time RT-PCR Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)
Africa 4
Fwd CCHF-III (CAA-GAG-GTA-CCA-AGA-AAA-TGA-AGA-AGG-C)
Real-time RT-PCR Rev CCHF-III-r (GCC-ACG-GGG-ATT-GTC-CCA-AAG-CAG-AC)
Probe CCHFprobe-1 (ATC-TAC-ATG-CAC-CCT-GCY-GTG-YTG-ACA)
Probe CCHFprobe-2 (TTC-TTC-CCC-CAC-TTC-ATT-GGR-GTG-CTC-A)

Fwd CCF-115F (AAR-GGA-AAT-GGA-CTT-RTG-GA)

Fwd CCF-131F (TGG-AYA-CYT-TCA-CAA-ACT-CC)
Nested PCR Rev CCF-759R (GCA-AGG-CCT-GTW-GCR-ACA-AGT-GC)

Fwd CC1a_for (GTG-CCA-CTG-ATG-ATG-CAC-AAA-AGG-ATT-CCA-TCT)

Asia 1 Real-time RT-PCR Rev CC1a_rev (GTG-CCA-CTG-ATG-ATG-CAC-AAA-AGG-ATT-CCA-TCT)
Probe CCHF-01 (CAA-CAG-GCT-GCT-CTC-AAG-TGG-AG)

Real-time RT-PCR Fwd CCHF-SF2 (GGA-VTG-GTG-VAG-GGA-RTT-TG)

Rev CCHF-SR2 (CAD-GGT-GGR-TTG-AAR-GC)
Probe CCHF-N2 (CAA-RGG-CAA-RTA-CAT-MAT)

Fwd CCF-115F (AAR-GGA-AAT-GGA-CTT-RTG-GA)

Fwd CCF-131F (TGG-AYA-CYT-TCA-CAA-ACT-CC)
Nested PCR
Rev CCF-759R (GCA-AGG-CCT-GTW-GCR-ACA-AGT-GC)

Fwd (GAT-GAG-ATG-AAC-AAG-TGG-TTT-GAA-GA)
Asia 2 Sybrgreen Real-time RT-PCR
Rev (GTA-GAT-GGA-ATC-CTT-TTG-TGC-ATC-AT)

Fwd CCS (ATG-CAG-GAA-CCA-TTA-ART-CTT-GGG-A)

RT-PCR
Rev 1 CCAS1 (CTA-ATC-ATA-TCT-GAC-AAC-ATT-TC)
Rev 2 CCAS2 (CTA-ATC-ATG-TCT-GAC-AGC-ATC-TC)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Real-time RT-PCR
Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)
Europe 1
Fwd CCF-115F (AAR-GGA-AAT-GGA-CTT-RTG-GA)
Nested RT-PCR
Fwd CCF-131F (TGG-AYA-CYT-TCA-CAA-ACT-CC)
Rev CCF-759R (GCA-AGG-CCT-GTW-GCR-ACA-AGT-GC)

Fwd CrCon1+ (RWA-AYG-GRC-TTR-TGG-AYA-CYT-TCA-C)

Rev CrCon1– (TRG-CAA-GRC-CKG-TWG-CRA-CWA-GWG-C)
Europe 2 Nested RT-PCR
Fwd CriCon2+ (ART-GGA-GRA-ARG-AYA-TWG-GYT-TYC-G)
Rev CriCon2– (CYT-TGA-YRA-AYT-CYC-TRC-ACC-ABT-C)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Europe 3 Real-time RT-PCR Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)

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 Chapter 3.1.5. – Crimean–Congo haemorrhagic fever

Clade Molecular assay combinations Primer and probe names (5’ → 3’ sequence)

Fwd CCRealP1 (TCT-TYG-CHG-ATG-AYT-CHT-TYC)

Rev CCRealP2 (GGG-ATK-GTY-CCR-AAG-CA)
Real-time RT-PCR
Probe (ACA-SRA-TCT-AYA-TGC-AYC-CTG-C)

Fwd CrCon1+ (RWA-AYG-GRC-TTR-TGG-AYA-CYT-TCA-C)

Rev CrCon1– (TRG-CAA-GRC-CKG-TWG-CRA-CWA-GWG-C)
Nested RT-PCR
Rev Fwd CriCon2+ (ART-GGA-GRA-ARG-AYA-TWG-GYT-TYC-G)
Nested Rev CriCon2– (CYT-TGA-YRA-AYT-CYC-TRC-ACC-ABT-C)

Fwd CCHF-SF2 (GGA-VTG-GTG-VAG-GGA-RTT-TG)

All Real-time RT-PCR
Rev CCHF-SR2 (CAD-GGT-GGR-TTG-AAR-GC)
Probe CCHF-N2 (CAA-RGG-CAA-RTA-CAT-MAT)

Fwd CCS (ATG-CAG-GAA-CCA-TTA-ART-CTT-GGG-A)

RT-PCR
Rev 1 CCAS1 (CTA-ATC-ATA-TCT-GAC-AAC-ATT-TC)
Rev 2 CCAS2 (CTA-ATC-ATG-TCT-GAC-AGC-ATC-TC)

Fwd CC1a_for (GTG-CCA-CTG-ATG-ATG-CAC-AAA-AGG-ATT-CCA-TCT)

Real-time RT-PCR
Rev CC1a_rev (GTG-CCA-CTG-ATG-ATG-CAC-AAA-AGG-ATT-CCA-TCT)
Probe CCHF-01 (CAA-CAG-GCT-GCT-CTC-AAG-TGG-AG)

(Data and table modified from Gruber et al. 2019)

2. Serological tests

Virus neutralisation assays, generally considered to be highly specific, are rarely used for CCHFV diagnosis.
Members of the Orthonairovirus genus generally induce a weaker neutralising antibody response than members of
other genera in the family Nairoviridae. Another drawback is the necessity to perform this assay in high biosafety
containment because it uses live virus (Burt et al., 1994; Rodriguez et al., 1997).

Currently, there are only a few CCHFV commercial kits for IgM or IgG by ELISA or immunofluorescence (IFA). These
are all designed for the human diagnostic market. However, it is possible to adapt these commercial ELISAs and
IFAs for serological testing in animals. In addition, some in-house ELISAs have been published for the detection of
CCHFV-specific antibodies in animals.

Diagnostic performance for humans have been compared between the methods using sensitivity, specificity,
concordance and degree of agreement with particular focus on the phase of the infection (Emmerich et al., 2021).
Available serological test systems detect anti-CCHFV IgM and IgG antibodies accurately, but their diagnostic
performance varies with respect to the phase of the infection. In the early and convalescent phases of infection, the
sensitivity for detecting specific IgG antibodies differed for the ELISA. Both test systems based on
immunofluorescence showed an identical sensitivity for detection of anti-CCHFV IgM antibodies in acute and
convalescent phases of infection.

IgM antibodies in livestock (sheep, goat and cattle) can be detected by using an IgM-capture ELISA. IgG antibodies
can be detected by an IgG-sandwich or indirect ELISA, and total antibodies can be detected by competition ELISA.
The benefit of competitive ELISA is the capacity to investigate different animal species, because they are host
species independent. Commercial kits for the detection of CCHFV-specific antibodies or the detection of viral
antigen are available. The limiting factor for the replication of these protocols in other laboratories is the availability
of antigens and (where relevant) specified monoclonal antibodies. Most of the tests described for livestock and wild
animals have not undergone a formal validation process (Mertens et al., 2013). One of the biggest challenges for
such validation studies is the availability of an adequate number of positive well characterised control samples.

For information on the availability of reference reagents for use in veterinary diagnostic laboratories, contact the
WOAH Collaborating Centres for Zoonoses in Europe and in Asia-Pacific.

C. REQUIREMENTS FOR VACCINES

There is no vaccine available for animals.

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*
* *

NB: At the time of publication (2023) there was no WOAH Reference Laboratory for Crimean–Congo
haemorrhagic fever (please consult the WOAH Web site:
https://www.woah.org/en/what-we-offer/expertise-network/reference-laboratories/#ui-id-3).

NB: FIRST ADOPTED IN 2014. MOST RECENT UPDATES ADOPTED 2023.

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