VSC 611 - Breeding of Vegetable Crops

VSC 611 BREEDING OF VEGETABLE
CROPS (2+1)
Course Content Prepared by
Dr. T.Uma Maheswari

Assistnat Professor
Department of Horticulture
Faculty of Agriculture
Annamalai University
&
Dr. K.Haripriya
Professor
Department of Horticulture
Faculty of Agriculture
Annamalai University
VSC 611 BREEDING OF VEGETABLE CROPS (2+1)
Objectives
To impart knowledge on principles and practices of breeding of

vegetable crops.
Theory
Origin- botany- taxonomy- cytogenetics- genetics- breeding

objectives- breeding methods (introduction- selection- hybridization-
mutation)- varieties and varietal characterization- resistance breeding for
biotic and abiotic stress- quality improvement- molecular markers-
genomics- marker assisted breeding and QTLs- biotechnology and their
use in breeding in vegetable crops- issue of patenting- PPVFR act of
the following crops:.
Unit I Breeding of solanaceous vegetables
Tomato, brinjal, chilli and sweet pepper.
Unit II Breeding of cucurbitaceous vegetables
Cucurbitaceous vegetables.
Unit III Cool season vegetables
Cabbage, cauliflower, carrot, beet root and radish.
Unit IV Bulb and tuber crops
Onion, garlic, potato, tapioca and sweet potato.
Unit V Greens and beans
Okra, moringa, peas, beans and amaranthus.
Practical
Modes of pollination and reproduction- pollen morphology and

viability- palanological studies- selfing and crossing techniques in
vegetable crops- assessment of variability- estimation of genetic
distance- techniques of hybridization in vegetable crops- emasculation
and hybridization- techniques of handling segregating progenies- D2
analysis- heterosis and combining ability- diallel and line x tester
analysis- assessment of character association- path analysis- study of
superior varieties and hybrids in vegetable crops- visit to vegetable
crops breeding centers and research institutes.
Lecture schedule
Origin- botany and taxonomy- genetics- cytogenetics- plant

genetic resources- anthesis- pollination- fertilization mechanism-
sterility and incompatability- constraints- breeding objectives- methods
and achievements of the following crops:
1. - 2. Tomato.
2.
3. - 4. Brinjal.
4.
5. - 6. Chilli.
6.
7. Sweet pepper.
8. Bitter gourd.
9. Ridge gourd.
10. Pumpkin.
11. Ash gourd.
12. Watermelon.
13. Muskmelon.
14. Cabbage.
15. Cauliflower.
16. Carrot.
17. Mid -semester examination
18. Beet root.
19. Radish .
20. - `21. Onion.
21.
22. Garlic .
23. - 24. Potato.
24.
25. Tapioca.
26. Sweetpotato.
27. - 28. Okra.
29. Moringa.
30. Peas.
31. Beans.
32. Amaranthus.
33. Issue of patenting, PPVFR act.
34. Marker assisted breeding and QTL.
Practical schedule
1. Study of pollination mechanisms, pollen morphology and viability

in solanaceous vegetable crops.
in cucurbits.
in crucifers.
4. Assessment of variability for vegetable improvement.
5. Estimation of genetic distance – D2 analysis
6. Estimation of heterosis and combining ability
7. Study of diallel and line x tester analysis
8. Study of correlation and path analysis
9. Floral biology and techniques of hybridization in solanaceous
vegetables.
10. Floral biology and techniques of hybridization in leguminous
vegetables.
11. Floral biology and techniques of hybridization in cucurbitaceous
vegetables.
12. Practices in breeding methods of cruciferous vegetable crops.
13. Practices in breeding methods of potato, tapioca and sweet potato.
14. Practices in breeding methods of root vegetables.
15. Practices in breeding methods of onion and garlic.
16. Practices in breeding methods of moringa and amaranthus.
17. Practices in breeding methods of okra.
Reference books
Dhillon, B.S., R.K. Tyagi., S. Saxena. & G.J. Randhawa. 2005. Plant Genetic
Resources: Horticultural Crops. Narosa Publ. House., New Delhi.
Fageria, M.S., P.S. Arya. & A.K. Choudhary. 2000. Vegetable Crops: Breeding and
Seed Production. Vol. I. Kalyani Publ.,New Delhi.
Gupta, S.K. 2000. Plant Breeding. Theory and Techniques. Vedam Publishers,
Solan.Harihar.
Ram. 2001. Vegetable Breeding. Kalyani Publishers, Ludhiana.
Kalloo, G. 1994. Vegetable Breeding – Combined Edition – Panima Book Publishers,
New Delhi.
Kumar, U. and M.J. Asija. 2004. Biodiversity: Principles and Conservation
Agrobios, Jodhpur.
Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of Vegetables,
Revised, ICAR, New Delhi.
Rai, N. and M. Rai. 2006. Heterosis Breeding in Vegetable Crops. New India Publ.
Agency, New Delhi.
Rana, M.K. 2011. Breeding and Protection of Vegetables. New India Publishing
Agency. New Delhi.
Singh, P.K., S.K. Dasgupta. and S.K. Tripathi. 2004. Hybrid Vegetable Development.
nternational Book Distributing Co., New Delhi.
Tomato L-1&2
Solanum lycopersicum
Solanaceae
2n= 2x =24
Tomato is the most widely grown fruit vegetable. It is marketed in fresh as well as
in processed form. It is a rich source of Vitamin A, Vitamin B, Vitamin C, Vitamin K and
minerals like Ca, P, K and Fe. They are the potential source of anti-oxidants like lycopene,
B-Carotene, Chlorogenic acid and the flavanoid naringenin present in fruit skin. The
meager amount of carbohydrate present is in the form of fibre. India ranks second in the
world production next to China with a productivity average of 21 t / ha. The demand for
this vegetable is constantly on increase as in recent times; focus on tomato based products
of food industry is catching up in Mediterranean and Arabian countries. Therefore the crop
is profitably cultivated throughout the year under protected conditions also.
Origin
The wild relatives of cultivated tomato are native of Western South America along
the Coast and High Andes from Central Equador, through Peru and Bolivia. Therefore,
South America is recognized as primary centre of origin. Domestication occurred in
Mexico of North America. One of the most dramatic changes through domestication is fruit
size. Wild tomato has tiny berries while modern tomato cultivars are large and succulent.
Mutations in about six Quantitative Trait Loci (QTLS) appear to be responsible for this
remarkable transformation in size. The gene loci ― fw 2-2‖ accounts for about 30 % of the
changes in fruit weight.
Related Genera
Capsicum
Cyphomandra
Related Species
It has been established through biosystematic studies that Lycopersicon is represented as a
‗Section‘ under the subgenus, Leptostemonum. Further clarity on gene substitutions has
lead to the fixation of recent changes in nomenclature as given below:
Solanum pimpinellifolium
S. lycopersicoides
S.pennellii
S.sitiens
S.habrochaites( L.hirsutum)
S. chmielewskii
S.peruvianum
S.cheesmaniae
S.neorichii ( L. parviflorum), S.rickii, S. juglandifolium, S. ochranthum
Solanum Chilense Solanum peruvianum S.Pennelli
Solanum pimpinellifolium S.cheesmaniae S.habrochaites
All these species of Solanum and a few others have either

through introgression or by gene transfer mechanisms has lead to modifications in present
day cultivated tomatoes (i.e) Improvement in
1) Resistance to pest
2) Resistance to Pathogens
3) Tolerance to humidity
4) Tolerance to drought
5) Tolerance to chilling temperature
6) Tolerance to high temperature
7) Tolerance to salinity
8) High Brix content
9) More of anti-oxidants
10) Thick pericarp
BOTANICAL VARIETIES
Solanum lycopersicum var commune Common tomato
Solanum lycopersicum var pyriforme Pear shaped tomato

Solanum lycopersicum var validum Upright tomato
Solanum lycopersicum var cerasiforme Cherry tomato
Solanum lycopersicum var Large leaved tomato plants
Grandifolium
Common Tomato Cherry Tomato
Habit
The crop is a short- lived perennial in its native habit, but grown as an
annual for production. A tomato breeder needs to be aware of the growth habit of
the crop to distinguish the variants. Besides, the objective determined for yield
characteristics fluctuates within certain limits based on the growth habit.
Growth Habit
The primary shoot of a young tomato plant produces 5- 10 leaves, after
which it produces a flower cluster. In indeterminate cultivars, the growth of this
primary shoot ends with the formation of first flower cluster. From the axils of last
leaf before the first flower cluster, a side shoot develops that grows upward. This
new shoot produces 3 more leaves before it ends in a flower cluster. This process of
new shoot development from the axils of last leaf initiated before every flower
cluster continues indefinitely, giving the appearance of a main stem with a flower
cluster between every three leaves.
In determinate cultivars, the process differs in that the upward growth above
the first flower cluster produces 0-2 leaves and a flower cluster but no further
vegetative shoots. But many side shoots arise from the main stem, giving the plant a
bushy appearance. Each of these secondary shoots eventually terminates in a flower
cluster.
Shoots of semi-determinate or intermediate plants produce several flower
clusters to the side of an apparent main stem, like indeterminates, but eventually the
shoot terminates in a flower cluster as in determinate plants.
Floral Morphology
Flowers are borne in three fashions (i.e) singles, in simple cyme and in
dichotomous cymes. Each flower cluster is called a ‘truss‘. Five to twelve flowers
are present in a truss. Individual flowers (each flower is borne on short pedicel
which is constricted at the middle point marking the position of abscission layer)
pendent, perfect and hypogynous, calyx or sepals are green, mostly six in number
that alternate with petals. They are persistent. Corolla or petals are rotate with a
short tube of six lobes and look yellow or pale yellow in colour. Stamens are also
six, each with two elongated comportments. Base is attached with corolla tube and
filaments are bright yellow joint at top forming a cone. There are six carpels that are
united and the basal ovary is typically six celled with a central fleshy placenta. The
style is first short and later elongates. Petioles, peduncles, stem, flower buds are
throughout covered with hairs.
Floral biology
The bottom most flower in a truss is the oldest and this will open first.
Anthesis is between 6 to 11 am. On the same day or the next day, anthers dehisce
longitudinally releasing pollen. This pollen easily get deposited over the stigma
placed in most varieties inside the anther cone. This structure of the flower by
nature favours self-pollination. Hence under green house condition mild shaking
of the truss is done artificially to improve the fruit set. The optimum temperature for
pollination is around 21oC.
Breeding objectives
The average productivity in India is 21t/ ha whereas the world productivity
is > 30 t / ha. We do have developed hybrids that yield around 100 t/ha. Any crop
improvement strategy adopted does not have choice of compromise on yield, which
is prioritized as main objective. Coupled with this trait is ‗earliness‘. Flowering
generally occurs within one month of transplanting. Further, ‗uniformity‘ in
flowering with genes for jointless pedicel is advantageous to facilitate harvesting
with less energy spent on labour or mechanical means. ‗Adaptation‘ to a particular
soil or climatic condition warranting tolerance to abnormal abiotic situations like
high or low temperature, humidity, drought, extended rainy days is advantageous.
(ie) varieties or hybrids with high ‗stability index‘ is preferred. Potential yield of
preferred cultivars are drastically brought down due to attack of pests including
nematodes and pathogens. Therefore development of high yielding varieties
exhibiting multiple disease resistance as well as tolerance to attack of common
pests including nematodes is popular among farmers.
Tomatoes are compulsorily subjected to either short or long distance
transportation. Hence, tough skinned types‘ possessing ripening inhibitors or non-
ripening genes is meritorious. Eye appeal by consumers rests with deep and
uniformly coloured, medium sized fruits having smooth surface. Those grown for
local consumption are preferred with good flavor and richness of vitamin C. Large,
thick fleshed, crispy textured fruits are procured by restaurants where salads,
display or food decorations are made with such types. Tomato plants meant for
processing sector are developed with good sugar: acid ratio, less seeds, more flesh
and good colour. Of late, purple skinned tomatoes have also been developed
holding nutraceutical value. Therefore a group of breeders or scientists work
together in the development of a variety.
Important tomato genes
CHARACTERS GEN
ES
Anthocyanin pigments Af
Potato leaved C
Elongated Fruit El
Exerted stigma Ex
Fascicated F
No fruit formation a,f,hl,

ms-31
High pigment Hp
Jointless pedicel J
Light green foliage Lg
Macro calyx Mc
Yellow flesh R
Compound inflorescence S
Tangerine T
Non ripening Nor
Aubergine Abg
Locules L
Breeding Methods
Breeding Methods
Conventional Breeding
Methods
 Introduction
 Selection
 Hybridization
 Pedigree method
 Bulk method
 Back cross method
 Heterosis
 Mutation
Non Conventional Breeding Methods
 Tissue culture
 Genetic engineering (Recombinant DNA technology)
1) INTRODUCTION:
 Seeds of improved varieties from one ecological area
are introduced into another and evaluated.
 Several introductions like Sioux, Roma, Marglobe,
Best of All and La Bonita became popular with farmers for
large scale cultivation
2) SELECTION:
 Mainly Individual Plant Selection is adopted where
chance variants, superior types from a mixed
homozygous population is selected.
 Effectively done to make maximum use of germplasm.
Source Selections
Meeruti Improved Meeruti
Local Cultivars Punjab Kesar , Angur Lata
Tip Top Arka Vikas
V-685 Arka Saurabh
Exotic Line HS 110
Ottawa-60 Arka Ahuti
UC 83 B Arka Aashish
Pearl Harbour (BWR 1) CO-1
AC 238 Pant Bahar
VC 8-1-2-1, CL 114-5-1-0 Arka Alok (BWR
5)
3) HYBRIDIZATION( Heterosis breeding)

 First tomato hybrid cultivar was ―Karnataka‖
introduced by Indo- American Hybrid Seed
Company (IAHS) in 1973.
Examples
 Arka Vishal (IHR-837 X IHR-932)
 Arka Vardan: IHR-550-3 X IHR-932
 Arka Shreshta: 15-SBSB X IHR-1614

 Arka Abhijit: 15-SBSB ×IHR-133
 COTH1: IIHR 709 x LE. 812
 COTH2: LCR 2x CLN 2123A
 TNAU Tomato Hybrid CO3: HN2x CLN 2123A
Varieties Parentage
Pusa Ruby Sioux X Improved Meeruti
Pusa Early Dwarf Improved Meeruti X Red Cloud
HS -101 Selection 2-3 X Exotic Cultivar
HS-102 S 12 X Pusa Early Dwarf
Punjab Chhuhara Punjab Tropic X EC 55055
Marglobe Marvel X Globe
Sel.1 Pusa Early Dwarf X HS 101
Sel.2 (HS 101 X Punjab Tropic) X (H-14
X Punjab Tropic)
Improved Cultivars developed by Interspecific Hybridization
Hisar Anmol S. esculentum X S. hirsutum
f.
Glabratum
Pusa Red Plum S. esculentum X S. pimpinellifolium
4) BACK CROSS METHOD

 Successfully used in breeding varieties resistant to
diseases such as Fusarium, Verticilium,
Stemphylium and Root Knot Nematode.
5) HYBRIDIZATION FOLLOWED BY PEDIGREE SELECTION

 Most common breeding method in tomato.
 Single plant selection is initiated in F2 and
continued through successive generations till pure
lines are obtained (generally till F6).
 Selection pressure is on heritable characters.
 EX : Pusa Ruby - Sioux X Improved Meeruti
6) HYBRIDIZATION FOLLOWED BY SINGLE SEED DESCENT
METHOD:
 SSD is a modification of bulk method.
 SSD is a system to rapidly develop pure
lines followed by selection among those pure
lines.
 This is classic procedure of having a single seed
in each plant, bulking the individual seeds, and
planting out the next generation.
 One or two seeds bulk are collected from each F2
plant and then F3 generation is grown.
 Now encouraged to be used by tomato breeder as
generation can be advanced in the off-season.
 Allows the maintenance of broad genetic base in
advanced generation Hybridization
7) MUTATION BREEDING
 PKM 1 -This is induced mutant from a local variety
called Annanji.
 CO3(Marutham)- It is an induced mutant from Co 1.
 Pusa lal Meeruti – Mutant from Improved Meeruti.
BREEDING FOR BIOTIC STRESSES
 Include disease and pest resistance.

 Existing old/new variety, land races and closely related species
provide genes for resistance.
 Genetics of resistance should be known before using a
particular breeding method.
 Emphasis should be given for development of complete resistance
by major genes.
 In tomato, the wild related species of Solanum are the
primary source of genes for resistance.
 Successful transfer of genes for resistance from related
species to the cultivated species is done by back
crossing and of late by genetic engineering.
Many wild species widely used as donors of genes for disease resistance
DISEASE RESISTANCE SOURCE RESISTANT VARIETIES
Buckeye rot S. pimpinellifolium Early Selection,KT-10,

KT-15, Flat Large Red,
Red Cherry
Fusarium Wilt S. hirsutum f. glabratum, Pant Bahar, BSS-20,
S.hirsutum, Roma, Meenakshi, Roza,
S. peruvianum, HS-110, Pan American for
S. pimpinellifolium race1 and Walter for race2
Leaf Curl Virus S. hirsutum f. glabratum, , H-24, H-36,Hissar
S. peruvianum, Gaurav, Hissar Anmol
S. pimpinellifolium
S. glandulosum
Spotted Wilt Virus S. hirsutum f. glabratum, , Pearl Harbour,
S. peruvianum, Red Currant
S. pimpinellifolium
Early Blight S. hirsutum f. glabratum, H-22, H-25, Solan Vajar,
S.hirsutum, Kalyanpur No.1
S. peruvianum,
S. pimpinellifolium
Late Blight S. pimpinellifolium Ottawa 30, Ottawa 31,

S. esculentum var. Red Cherry, Early Market
cerasifolrme
BREEDING FOR ABIOTIC STRESSES
Include environmental conditions such as;
 Low and high temperature

 Drought conditions
 Flooding
 Moisture stress
 Soil salinity
 Water logging conditions
A few species tolerant to abiotic stresses are
S. cheesamanii Salt tolerance, heat tolerance for

fruit
Set
S. pimpinellifolium Heat tolerance for fruit set, drought
Tolerance
S. chilense Drought tolerance, cold resistance
S. hirsutum Cold tolerance, chilling tolerance,
salt Tolerance
S. pennelii Drought tolerance, salt tolerance
S. lycopersicoides Cold tolerance
Abiotic stress resistant varieties
VARIETY FEATURES
Pusa hybrid-1 Fruit set upto 28o C (high) night
Temperature
Pusa Sadabahar Fruit set at both low (6o C) and high

(30o C) night temperature
Sabour Suphala Salt tolerant at seed germination stage
Arka Vikas Tolerant to moisture stress
Varietal Achievement
VARIETY ORGANIZATIO PARENTAGE FEATURES

N
Pusa Ruby IARI, New Delhi Sioux X Indeterminate, early,
Improved firm, medium size,
Meeruti uniform ripening
Pusa Early IARI , New Delhi Improved Meeruti X determinate Plants
Dwarf Red Cloud, dwarf, early maturing,
medium size
HS 101 HAU , Hisar Sel. 2-3 X An exotic A very promising

culture variety, determinate,
dwarf, fruit medium
size juicy, wide
stability
HS102 HAU ,Hisar S 12 X Pusa Extremely early,
Early Dwarf Fruits medium to
small, round
juicy, thin pericarp
HS110 HAU , Hisar Selection from an Determinate, late,
exotic line potato leaf type,
fruit large, highly
suitable for table
purpose.
Hisar Arun HAU , Hisar Pusa Early Dwarf Extremely early and
X K1 high yielding,plant
dwarf,
fruimedium to large
Hisar Lalima HAU , Hisar Pusa Early Dwarf Early, determinate,
X HS 101 fruits round, large,
high Yielding
Hisar Lalit HAU , Hisar HS 101 X Semi- determinate,
Resistant early, fruit round,
Bangalore medium to large,
resistant to root
knot nematode
Hisar Anmol HAU , Hisar Hisar Arun X L. Field resistance to
Hirsutum f. tomato leaf curl
glabratum virus, plant
determinate, fruit
medium size
PKM 1 TNAU, Mutant of Annanji Long distance
Coimbatore Transportation
CO 3 TNAU, A mutant of CO 1 Determinate, fruit red
Coimbatore round, medium size
COTH1 TNAU, Coimbatore IIHR 709 x LE. 812 Fruit are acidic
(0.61%)
High yielding
COTH2 TNAU, Coimbatore LCR 2x CLN 2123A Resistance to leaf curl
virus disease.
TNAU Tomato TNAU, Coimbatore HN2x CLN 2123A Suitable for high
Hybrid CO 3 density planting.
Resistant to leaf curl
virus diseases.
KS 2 Kalyanpur Determinate, early,
round, high yielding
Punjab PAU , Ludhiana EC 55OO5 X Determinate, dwarf,
chhuhara Punjab Tropic high yield, pear
shaped fruit, suitable
for long
transportation .
Sel .120 IARI , NewDelhi Semi- determinate,
late, fruit large, resistant
root knot nematode
Pant T3 GBPUA&T, Pure Line SelectionPlant semi-
Pantnagar determinate, fruit
medium round,
Smooth
Arka Vikas IIHR , Bangalore A selection from a Plant indeterminate,
variable population fruit medium large,
American variety Tip suitable for fresh
Top market
Arka Saurabh IIHR , Bangalore Selection from a Semi determinate,

line V-685, suitable for both
introduced from processing and fresh
Canada Market
Arka IIHR , Bangalore Triple disease
Rakashak resistance
to ToLCV, Bacterial
Wilt and early blight.
Arka Samrat IIHR ,Bangalore Triple disease
resistance
to TLCV, Bacterial
Wilt and early blight
Pusa Gaurav IARI , NewDelhi Fruit yellowish red,
good
for processing and
long distance
transportation
Pant Bahar GBPUA&T.Patna Bushy branched,

gar flattish round, medium
Fruit
Pusa Hybrid1 IARI , Newdelhi F1 hybrid Determinate
plant, prolific
bearing, round fruits
Pusa Hybrid 2 IARI, New delhi F1 hybrid Semi –
determinate, dwarf
plant, medium sized
fruits
IMPORTANT HYBRIDS AVAILABLE IN PRIVATE SECTOR
Century Century-12, Maitri, Rishi
Karnataka, Mangla, Vaishali,

Indo American
Rupali, Naveen, Rashmi, Sheetal
MTH-1, MTH-2, MTH-6, MTH-15, MTH-

Mahyco
16, S-28, Sonali
NS-386, NS-815, Summerset, Cross

Namdhari
B, Gotya
Nath NA-501, NA-601
Nijjar NH-15, NH-25, NH-38
Pioneer LIHB-230
Sandoz Learika, Rasika, Avinash 11
Sungrow Arjuna, Krishna, Bhim
Sutton Sutton Grom, Prolific
Beejo Sheetal BSS-39, BSS-20, BSS-40, BSS-90

18
VARIETIES OF TOMATO
COTH1 COTH2
Arka Abha Arka Abhijit
Arka Raskshak Arka Samrat

19
Arka Saurab Arka Vikas
Vaishali Naveen
TNAU Tomato Hybrid CO3 PKM 1
Technology of production of hybrid seed
Hand emasculation and hand pollination is to be practiced. Two parents having good
combining ability are selected. Varieties maintained by selfing can be taken as parents. In a
few cases 2-3 years selfing is done to maintain the purity. A large number of such parents
crossed in different combination and resultant hybrids evaluated at one or more location. In a
20
few cases sca is studied to select a potential hybrid. Practically, superiority is judged by per
se performance.
Hybrid seeds largely produced by hand emasculation and hand pollination. Seed
plants and pollen parents are grown under healthy condition in 12:1 ratio. Emasculation of
seed parent is taken 12-15 hr before anthesis, by forceps, needle or fingernail. Generally
emasculation is done in the afternoon and pollination is done in the following morning.
Pollen collected from anthers by needle, forceps or electric vibrator. Generally fresh pollen is
used. Pollen can be stored for 2-3 days under normal condition. Soon after pollination, 2-3
sepals of the pollinated flowers are removed for easy identification. (No need to put bag or
cotton). 4-5 days - ovary start swelling. More than 90% fruit set after hybridization.
Questions
1. Name botanical varieties of tomato
2. What are the breeding objectives in tomato breeding?
3. What is the chromosome number of tomato?
4. Name multiple resistant varieties in tomato
5. List out tomato varieties released from IARI.
References
Kalloo, G. 1994. Vegetable Breeding – Combined Edition – Panima Book Publishers, New Delhi.
Vegetable Breeding. Kalyani Publishers, Ludhiana.
L- 3&4
Brinjal
Solanum melongena
Solanaceae
2n=2x=24
Brinjal is proved to have originated in India with many diversified forms and China is
the secondary centre of diversity. In the south East Asian Countries the crop finds a place in
native medicine besides its versatility in consumption as vegetable as it is simple and
delicious. In addition to bringing in a unique texture and mild flavour to recipes brinjal brings
a load of health benefits. It is a nutrient dense food as it contains considerable amount of
Vit.C, Vit. K folate, Manganese and Potassium. It is high in fibre and polyphenols, solasodine
which helps in fighting against a type of cancer. Rich anthocyanin content also contributes to
this quality. It is reported that ‗Nasunin‘ is a type of antioxidant predominantly found in
fruits. Owing to such merits, the crop is widely cultivated in all tropical and subtropical parts
of the world. Moreover native cultivars respond to ratooning and give satisfactory yield.
Breeding objectives
21
Preference for fruit colour, shape and size determines market potential in any
particular region. Therefore development of high yielding varieties with preferred fruit
characteristics is given importance. Since drastic yield reduction occurs due to the major pest,
shoot and fruit borer, disease, little leaf; resistance breeding for incorporation of interested
specific genes in otherwise good cultivars are progressing. Further, development of tolerant
genotype for drought, salinity is advancing. Related rootstocks are also utilized in breeding
strategies. Since technique of grafting is successful in brinjal.
Related genera
Capsicum
Cyphomandra
Related species
Solanum sisimbrifolium- ancestral form, leaves are variegated.
S. aethiopicum
S. macrocarpum- leaves are edible
S. indicum
S. trilobatum
S. viarum
S. nigrum
S. xanthocarpum – all are used in native medicine
Botanical varieties:
S. melongena variety esculentum – bears round fruits
S. melongena variety serpentium- bear long fruits
S .melongena variety depressum – dwarf plant stature.

22
Solanum melongena var esculentum S .melongena var serpentinum
S. melongena var depressum
The crop is cultivated as annual and grows to a height of 1 to 2 m. Flowers are in cluster
Individual flowers are perfect. Corolla is purple mostly and white is also seen rarely. Anthers
are dark yellow arising from base of petals. They are lengthier than filaments. Ovary is
superior and length of style in individual flower varies rendering the chances of fruit set per
cent to vary accordingly. This phenomenon is called heterostyly where stigma either
coincides the tip of anthers or stays just below it or slightly protrudes making it visible
outside. The other two forms namely pseudo short and true short style flowers do not set
fruits whose style length is just the half or quarter of anther length. As the dehiscence of
anthers takes place in their tips( apical dehiscence) and they are arranged together in the top
portion, the dispersed pollen doesn‘t get deposited over the stigmatic lobes, which results in
no fruit set. Genetic as well as physiological factors are reasoned for distribution of varied
style length in a particular variety grown under specific climatic condition.
23
Long styled Medium styled Short styled
Floral biology
Corolla or petals are showy in purple colour. Calyx or sepals are 5 and united at the
base. They are persistent. Anthers are bilobed, dark yellow attached to base of petals by short
filaments.
Flowers open in morning hours from 6 am to 8 am under tropical climate. Anthers

dehisce through apical pores on the same day after an hour of anthesis. Stigma is receptive a
day before flower opening and on the day of anthesis. Stigmatic fluid dries up after bright sun
shine on the next day of flower opening.
Breeding methods
• Pure line selection
IIHR
• Arka Shirish-Pureline from IIHR-194.
• Arka Kusumakar-from IIHR- 193.
TNAU
• Co-1
• Co-2(from Varikathirikaai)
• MDU-1(From kallampatti local)
• VRM-1(From Elavambadi village of vellore)
• KKM-1(From Kulathur local)
• Pedigree method
Arka Nidhi
24
• Bulk method
• Modified pedigree (single seed descent)
• SINGLE LINE SELECTION
PPI(B)-From Karungal local type.
PLR-2 - From Sevanthampatti local).
• Combination of bulk and pedigree methods
• Backcrossing
• Heterosis breeding
• Mutation breeding
PKM-1(From Puzhudhikathiri).
 Heterosis is commercially exploited
• Hand emasculation and pollination is practiced.
Methods of crossing
• Extent of cross pollination is about 29 %.
• Hence , called as often cross pollinated or facultative cross pollinator.
• Out crossing by means of insects like bumble bees,wild bees & domestic
bees(entomophilous).
• Use of male sterility – ‗Blackey‘ – male sterile
• Inducing male sterility through growth regulators and gametocides is achieved.
Interspecific hybridization
• Related species of S. melongena for disease resistance
• Among 22 Indian species, 5 are closely related
• S. melongena,
• S. incanum (S. coagulans)
• S. xanthocarpum,
• S. indicum
• S. maccani
25
• S. melongena – readily crossable with S. melongena var. incanum
• Hybrid between S. melongena and S. sisymbrifolium – failed to produce viable

seeds
• Sources of resistance
Species Resistance
S. melongena var. incanum Bacterial wilt ( Pseudomonas

solanacearum)
S.xanthocarpum, S. sisymbrifolium, Fruit rot( Phomopsis vexanas)

S.nigrum
S. sisymbrifolium Root knot nematode
S. indicum Wilt ( Verticillium alboatrum)
S. torvum, S.mammosum, Spotted beetle( Epilachna

S.khasianum vigintioctapunctata)
• Intergeneric grafts
• Solanum, Capsicum and Lycopersicon are intergraftable
• Side grafting and patch budding – successful
• Problematic areas – one genus thrives well
Varieties and hybrids
Arka Anand
It is a high yielding F1 hybrid with resistance to bacterial wilt. Average fruit weight
is 50-55 g. Yields 60-65 t/ha in 140-150 days.
Arka Anand
Arka Neelkanth
26
Violet blue glossy fruit skin with green purple calyx. Resistant to bacterial
wilt. Duration 150 days. Yield 43 t/ha
Arka Neelkanth
ARKA NIDHI (BWR)
Resistant to bacterial wilt. Duration 150 days. Yield 48.5 t/ha.
Arka Nidhi
Arka Shirish
Tall plants with white flowers. Fruits green, extra long. Solitary bearing habit.
Arka Shirish
Some popular brinjal varieties and hybrids
Name Methods Organization Attributes
Arka Sheel PLS from Kodagu IIHR, Bangalore Plants well branched
local and erect, Fruits
medium long, tender,
deep purple. Less
seeds, yields 39t/ ha
in 110-120 days.
27
Arka PLS from Karnataka IIHR, Bangalore Dwarf plants, fruits

Kusumakar local selection small, Finger shaped,
light green in colour
and borne in clusters
of 5 to7. Yield 45 t/
ha in 110-120 days
Pusa Purple Selection from Batia IARI, New Delhi Semi erect, dwarf
Long , Punjab plants, 20-25 cm
long purple fruits,
suitable for autumn
and summer
planting, susceptible
to bacterial wilt.
Pusa Purple Selection from local Medium early, erect,
Cluster material IARI, New Delhi purple pigmentation
on stem, leaves
purple and non
spiny, fruits in
clusters, deep purple
, moderately resistant
to bacterial wilt.
Pusa Kranti (PPLxHyderpur)x IARI, New Delhi Plants medium tall,
Wynad Giant purple pigmentation
( Hybridization on young leaves, non
followed by spiny, fruits oblong,
Pedigree selection) thick, less seeded,
Pusa Bhairav Complex IARI, New Delhi Fruits dark purple,
non spiny, glossy,
resistant to
phomopsis fruit rot
MDU1 PLS from local ACRI, Madhurai Fruits round, bright
Kallampatti purple, less seeded,
yields 30 t /ha
CO2 PLS from local TNAU, Coimbatore Fruits oblong, with
variety, Varikathiri dark purple streaks
under pale green
background, without
spines on the calyx
surface. Yields 35t/
ha in 150 days.
28
PKM1 Induced mutant from HCRI, Periyakulam Fruits small, oblong

Puzhuthi Kathiri ovate with green
stripes, suitable for
rainfed cultivation.
PLR1 Selection from VRS, Palur(TNAU) Egg shaped fruits,
Nagpur ecotype bright glossy purple
colour, yields 25 t/
ha.
Pant Samrat Selection from local Pantnagar Medium long fruits
cultivar ,Agricultural in clusters,resistant
University to bacterial wilt,
tolerant to shoot and
fruit borer.
KKM1 Selection from local Agricultural White colured egg
type of Kulathur College, shaped fruits, cluster
Killikulam(TNAU) bearing (2-4) with
green calyx. Yields
36t/ ha.
PPI1 Single line selection Long plae green
from Karungal local fruits, less seeded,
type bitter less,yields
about 50 tonnes/ ha
in a crop duration of
185 days.
F1 hybrids
Pusa Anmol PPLx Hyderpur IARI, New Delhi Gives 80 % more

yield than PPL.
Azad Hybrid Azad b1x Kalyanpur C.S.Azad Agri Early fruiting, bright
3 University, purple coloured
Kaliyanpur, (UP) fruits, less prone to
fruit and shoot borer
infestation, yields 45
t/ha.
Arka Navneet IIHR 221x Supreme IIHR, Bangalore Fruits round to oval,
big sized, black
purple, yields 65-75t/
ha.
NDBH1 PBR 91-1xK202-14 Plant semi erect, non
spiny, round purple
fruit, soft textured,
less seeded.
29
Questions
1. ________________ is the origin of brinjal.
2. What is heterstyly?
3. What are the botanical varieties of brinjal ?
4. Name some brinjal varieties released from IIHR
5. Name some brinjal varieties resistant to bacterial wilt.
References
1. Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of Vegetables,

2. Kumar, N. 2006. Breeding of Horticultural crops: Principles and Pracitices.

New India Publishing Agency, Pitam Pura, NewDelhi.
30
L- 5&6
Chilli
Capsicum annuum L.
Solanaceae
2n=24
It is an important vegetable cum condiment. The pungent or hot types are
grouped under Red pepper or hot pepper (Capsicum annuum L.), whereas the less
pungent types are grouped under sweet pepper (Capsicum annuum var.annuum)
locally called as ‗Kudai Milagai‘, which are used as vegetable.Pungency of the chilli
is due to the presence of Capsaicin C18 H27 NO3 and the coouring principle is due to
the presence of Capsanthin.
ORIGIN & DISTRIBUTION
The domestication of Capsicum annuum L. first occurred in Central America,

most likely in Mexico where archaeological remains from almost 7000 B.C. indicate
that pepper were used by man even before the advent of Agriculture. Chillies were
introduced by Portuguese into South India probably in 16th century as evidenced by
the fact that there were three races of chillies being cultivated in India by 1542. By the
end of 19th century, its cultivation spread throughout India. Chilli is mainly cultivated
in Brazil, Mexico, Spain, South and Central America, China and India. In India,
Andhra Pradesh, Maharashtra, Karnataka, Tamilnadu and Himachal Pradesh grow the
crop extensively.
CULTIVATED SPECIES ARE:-

 C. annuum - Mexico
 C. frutescens - Amazonia
 C. chinense - Amazonia
 C. pendulum - Peru and Bolivia
 C. pubescens - Peru and Bolivia (Extinct)
 C. pendulum - C. baccatum var. pendulum ,
 C. microcarpum - C. baccatum var. baccatum
31
Capsicum pubescens
Capsicum baccatum- Bishop’s crown
Capsicum frutescens -Upright fruits
Capsicum chinense
32
Horticultural classification of Pepper

Groups Size (inch) Pungency of Common Uses
cultivars
Bell 3 to 5 X 2 to 4 California and Yolo Fresh market, salad, and canning

wonder (non & few)
Pimiento 2.5 to 5 X 2 to 3 Pimiento and pimsan Fresh market, salad, and canning
(non)
Squash 1 to 2 X 2 to 4 Yellow cheese, (non) Processing, canning, pickling
Ancho 4 to 6 X 2 to 3 Ancho, Mexican Powder, dried chilli

chilli(mild)
cayenne 5 to 10 X 0.5 to 1 Cayenne long red(high) Dried, powder, sauce, salads
Jalapeno 1.5 to 3 X .75 to 1 Jalapeno (high) Green,
Anahelm 5 to 8 X 0.75 Paprika (non pungent) Color, canning

to1.75
Cuban 3 to 6 X 0.5 to 2 Cuban, golden greek Salads, pickled

(mild)
Small 1. 5 to 3 X0 .25 to Fresno chilli, Serrano Seasoning and Sauce

hot 1 (high)
Cherry 0.5 to 2 Pungent Pickling
Short 2 to 3 X 1 to 2 Cascabella, floral gem Processing, pickling

wax
Morphology of fruit
 Fruit type is berry
 Capsaicin glands – between placenta and endocarp - produce capsaicin
 Calyx – Persistent calyx
 Seeds – rich in lecithin, capsaicin absent, hotness due to absorbance
 Placenta – metabolic exchange fruit to seed
 Chilli hotness measured by SHU (Scoville Heat Unit )
 Bhut jolokia – 1,001,304 SHU
 Naga morich – 1,598,227 SHU
33
Fruit structure
Flowers
 Flower - Actinomorphic, Bisexual, Pedicellate, Hypogynous
 Gynoecium - Superior ovary,
 Androecium - Stamens 5-6, Epipetalous
Flower structure
Floral Biology
 Both self and cross pollination occur, the latter being about 16% by bees, ants and
thrips. Flowering begins 1-2 months after planting and it takes another month for
fruiting.
 Flowers open in the morning between 5.00 a.m. to 6.00 a.m. Anthers normally
dehisce between 8.00 a.m. to 11.00 a.m..
 Flower opening and anther dehiscence depend on the weather conditions to a large
extent.
 During cool as well as cloudy days, the flower opening is delayed.
 Pollen is fertile on the day of anthesis and stigma is receptive for about 24 hours after
flower opening.
BREEDING OBJECTIVES:
1. Earliness
34
2. Desirable fruit shape and size (obovate and round fruit in bell pepper and long fruits in
chilli).
3.Superior fruit quality (pleasing flavour, high sugar/ acid ratio, high pigment content and
high capsaicin).
4. Resistance is to diseases (fruit rot, cercospora leaf spot, powdery mildew, bacterial leaf
spot, phytopthora root rot, root knot, common TMV).
5. Resistance to insects (thrips, mite, aphid, fruit borer)
6. Resistance or tolerance to abiotic stress (heat, water stress, salinity etc.)
BREEDING METHODS:
Pure line selection:
This method is applicable to land races or local cultivars being grown by farmers. Eg .,
G1, K1, CO.1, CO. 2, Sindhu, PLR. 1
Pedigree method:
It involves selection of superior plants following hybridization between superior cultivars.

Eg., Andhra jyothi(G5), pusa jawala, Punjab lal, Jawahar 218, K2, PKM.1, Pusa Sada Bahar
Back cross method:
It is used to transfer single gene or few genes from primitive cultivars or wild forms to
leading cultivars.
Heterosis breeding:
F1 hybrids are popular in USA and Europe and gaining popularity in India after the
initiation. A hybrid MS12x LLS has been identified for commercial production at PAU ,
Ludhiana.
F1 hybrids in chilli
Public sector
PAU(Ludhiana) : CH-1, CH-3
IIHR-: Arka Suphal, Arka Meghana, Arka Sweta, Arka Harita
Private Sector
Hung Nong : Delhi Hot, Hot green, Skyline
Mahyco: : Tejaswini
Sandoj : Agni
Scoul :Champion
35
Mutation breeding:
Found to be effective and efficient breeding tool in pepper. MDU-1 has been
developed at the Agriculture College, Madurai by treating the seeds of K.1 by gamma rays @
30 Kr. It posses compact plant type and determinate growth habit with fruits borne in clusters
of 4-9 at nodes as against single fruit borne at nodes in K.1 or K.2 varieties. Duration 205-
215 days with a dry pod yield of 1809 kg/ ha.
Sterility in chilli
First male sterile( ms) plant was isolated from an Indian accession.
GMS:
The ms-509 was renamed as ms-10. This ms-509 line (bell pepper type) of Dr.
Pochard was introgressed in 3 chilli genotype, viz., MS-12, MS-13 and MS-41. MS-12
Line has been developed by transferring ms-10 gene into cultivar ―punjab Lal‖ through
backcrossing. Using MS-12 line 3 chilli hybrid CH-1, CH-3 and CH-27 has been released
by PAU.
CGMS:
First reported in an introduction from India (PI-164835). Chilli CGMS lines (CCA-
4261) introduced at the IIVR from AVRDC is utilized to produce hybrid – Kashi Surkh
(CCH-2). IIHR, Bangalore has also developed CGMS based hybrids i.e. Arka Meghna
(MSH-172),Arka Harita (MSH-149) and Arka Sweta (MSH-96).
Genes considered too important for breeders
Important Capsicum genes
Gene symbol Phenotype
A Anthocyanin- basic gene for purple colour

in foliage, incompletely dominant
B Beta carotene-high in mature fruit
T High Beta carotene, complementary with B
Y Yellow or orange mature fruit colour
Ys Yellow spot on corolla of C.pendulum
Yt1. Yt2 Young expanding leaves are yellow
dw1, dw2 Dwarf plant

36
HL Hairless
Bs1 Bacterial Spot resistance
N Root knot nematode resistance to

M.incognita
O Oblate or round fruit shape dominant over

elongate
P Pointed fruit apex incompletely dominant

over blunt
ms Male sterile
Crossing technique:
Emasculation and pollination in chilli
Crossing technique
A bud one day prior to anthesis should be selected for emasculation.
Emasculation should be carried out without damaging female reproductive parts and bagged
it.
At a same time pollen should be collected from the fully opened male flowers.
Gently transfer the pollen to the stigma, bagging and tagging should be done simultaneously.
37
Varieties & Hybrids
Variety Institution Parentage Breeding Remarks

Methods
Arka Haritha IIHR, CMS F1 Green & dry;

Bangalore Resistant to
Powdery mildew &
virus
Arka Sweta CMS F1 Dual purpose
Arka Meghana CMS F1 Dual purpose;

tolerant to Powdery
mildew & virus
Arka Suphal CMS F1 Indeterminate, PM
PMR 57 resist, Tolerant to
virus
Arka Lohit Ihr324local PLS Tolerant to powdery
collection mildew
38
Arka Abhir Devanur PLS Paprika variety

dubha suitable for
extraction of colour
Kashi Anmol IIVR, IC-523656, Introduction Dwarf , Umbrella
Varanasi Sri Lanka type
Kashi Heterosis Tall indeterminate(

Surkh(CCH-2) breeding( F1) 110-120 cm)
Kashi Heterosis indeterminate( 80-

Early(CCH-3) breeding( F2) 100 cm)
Pant C1 GP Pant NP46Ax PLS Tolerant to virus,

Agricultural Kanthari erect bearing
Uinversity,
Pantnagar
Pant C2 NP46Ax PLS Pendant fruits

Kanthari
Pusa Jwala IARI, New NP46Ax Puri Pedigree Tolerant to thrips,

Delhi Red mites, aphids
Pusa Pusa Jwalax Pedigree Virus resistant

Sadabhahar Ic31339
CH.1 Punjab MS12xLLS GMS Tolerant to virus

Agricultural and fungi
University,
Ludhiana
CH.3 MS12xS- F1 Mild Pungent,

2530 Processing
Punjab Lal Perennial long Resistant to CMV,

Red TMV, LCV.
39
K1 K2
CO1 CO2
CO3 CO4
KKM1 PKM1
40
PKM2 Arka Harita
Arka Lohit Arka Meghana
Arka Suphal PMR 57 Arka Sweta
Questions
1. What is the pungency pricnciple of chilli ?
2. What is the origin of chilli?
3. What are the breeding objectives of chilli?
4. What are the related species of chilli?

41
5. What is the breeding system in chilli?

References
Ram. 2001. Vegetable Breeding. Kalyani Publishers, Ludhiana
CAPSICUM
L-7
Scientific name : Capsicum annuum var. grossum
Common name : Bell pepper, Sweet pepper.
Family : Solanaceae
Chromosome number : 2n=2x=24
Origin :South America
Importance of the crop:
 Bell pepper is grown as autumn- winter crop in the plains and summer crop in the hill
regions of India.
 It is consumed both in green mature and ripe form, salads, cooked, mixed and stuffed
vegetable.
 It is widely used in the preparations of pickles, sauces, soups and stews.
Floral biology and pollination:
Flower:
 The flowers of bell pepper are larger than chilli.

 They are bisexual located in the nodes of the stem.
Anthesis : 5:30 to 6:30 AM
Pollination : Self-pollinated crop; cross pollination occurs to an extent of

62 %. Insects are responsible for natural crossing. Therefore
treated as often cross pollinated crop
Stigma receptivity : 2 hrs before and 2 hrs after anthesis
Cytogenetics:
 Chromosome number : 2n=2x=24

 Somatic chromosome number : 2n= 24
42
 Polyploids - 2n=36
 Aneuploid -2n=25 has been reported.
Breeding objectives:
 High yielding and earliness.

 Good quality: high vitamin C content, high oleoresin content, pigment content,
pleasing flavour, shiny fruits, pungent and non-pungent.
 Desirable fruit shape and size: oblate or round.
 Resistance to diseases like fruit rot, cercospora leaf spot, powdery mildew, common
TMV, etc.
 Resistance to insects like thrips, mites, aphids, fruit borer.
 Resistance to abiotic stresses like heat, water stress, salinity.
Breeding methods
Conventional breeding methods:
Introduction, Selection, Pureline selection, Pedigree method, Backcross method,

Heterosis and Mutation breeding.
Non-conventional breeding methods:
Tissue culture and Genetic engineering.
Introduced varieties:
California Wonder, Yolo Wonder : Introduction from USA by IARI, New Delhi World
Beater, Chinese Giant : Introduced by IAHS, Bangalore.
Varieties developed by selection:
 Arka Mohini : Selection from Titan of USA by IIHR, Bangalore.

 Arka Gaurav : Selection from Golden California wonder of USA by IIHR,
Bangalore. Indeterminate variety tolerant to bacterial wilt.
 Arka Basant : Selection from Hungarian variety ―Soroksari‖ by IIHR, Bangalore.
Excellent keeping and cooking qualities.
 Nishant 1 : A selection from Capsicum Sel-2 (SKUAS&T, Srinagar)
Arka Mohini
43
Arka Basant
Arka Gaurav
44
California wonder
Pedigree method:
Selection of superior plants in the segregating generations. Hybridization is done between

superior cultivars along with maintenance of pedigree record.
Some of such cultivars are
 Spartan Garnet: (California Wonder × Dwarf Pimiento Selection from variety

Santanka)
 Spartan Emerald: (Morgold × California Wonder)
 Sonnette: An F2 line (Morgold × California Wonder) × Keystone Resistant Giant.
Backcross method:
 In bell pepper generally 4-5 backcrosses are required to produce near-isogenic lines
for the novel gene in the pure line.
 Backcrossing is used whenever disease resistance, improvement of male sterile line or
any other simply inherited morphological trait is to be transferred because its
application is convenient in bell pepper.
 Incorporation of resistance (powdery mildew) from C. baccatum var. pendulum.
Mutation breeding:
Direct mutant cultivars:
MDU 1 developed from TNAU by gamma rays induction inthe year 1976. Plants are
compact, high yield and capsaicin content.
Heterosis breeding:
First hybrid variety was Bharat from IAHS.
Private sector hybrids:
• Mahabarath
45
• Chocolate wonder
• Mamatha
• Lakshmi
• Super gold
Public sector hybrid:
 Pusa Deepti (Kt - 1): hybrid between Yolo Wonder and Reunion Yellow, released by
IARI Regional Station, Katrain (HP). High yielding, tolerant to bacterial leaf spot and
anthracnose.
 Solan Hybrid - 2: Released by YSPUHF, Solan. Resistant to fruit rot and virus.
 Solan Hybrid - 1: Released by YSPUHF, Solan.
 Solan Bharpur: Released by YSPUHF, Solan.
 KTCPH - 3: Yolo Wonder x HL-201, released by IARI Regional Station, Katrain
(HP).
 KTCPH - 5: Yolo Wonder x EC- 143570, released by IARI Regional Station, Katrain
(HP).
 Punjab - 27
Varieties / Hybrids Breeding methods Features

Sweet Banana - -
California Wonder Introduction from USA Plants vigorous, upright, prolific
bearer, medium thick flesh, Early
harvest in 70 days
Yolo Wonder Introduction from USA -
Arka Mohini Introduced from USA Determinate, medium large fruits,
dark green skins
Arka Gaurav Introduced from USA Dark green fruits, erect, 4-5 lobed
thick flesh, Tolerance to bacterial
wilt
Arka Basant Introduced from Hungary Proloific bearing,
good export potential, creamish
white colour
King of North - -
Pusa Deepthi Yolo Wonder x Russian F1 Hybrid
Yellow
Kt – 1 -
Bharath F1 hybrid 1st F1 Hybrid (1973)by IAHS,
Bangalore
Mahabharath Tolerant to TMV
Chocolate Wonder Dark brown colour fruit
Mamatha Red colour fruit
Lakshmi Red colour fruit
Super Gold Yellow colour fruit
46
Indam bharath Yolo wonder
Lakshmi
Super gold
47
Chocolate wonder
Mahabarath
Tissue culture:
Bulgarian cultivars
• Hebar
• Stryama
Indian cultivar
• Mathania
Important donors for resistance to disease and pests
Disease/ insect Resistant sources

Fruit rot C. chinense Accs 1555, Chinense Giant
Cercospora leaf spot California wonder, Hungarian Wax
Root Knot Santaka
Leaf curl Pant C1, Puri red, Puri Orange
Thrips Caleapon Red, Chamatkar
Mite Kalyanpur Red, Punjab Lal
Aphid Kalyanpur Red x 1068
48
Questions :
1.Name a F1 hybrid f Capsicum
2. -----------------is the resistance source for fruit rot.
3. What is the Chromosome number of Capsicum?
4. What is the mode of pollination in Capsicum?
5. What is the time of anthesis in capsicum?
References
Ram. 2001. Vegetable Breeding. Kalyani Publishers, Ludhiana
BITTER GOURD
L-8
Scientific name : Momordica charantia L.
Family : Cucurbitaceae
Origin : Indo-Burma region
Chromosome No : 2n=22
Importance
1. The vegetable Momordica charantia L. belongs to cucurbitaceae, is known variously

as bitter gourd, balsam pear, bitter melon, bitter cucumber and African cucumber.
2. It occupies first rank among the cucurbits in respect of iron (1.8mg/100g), vitamin C
(88mg/100g).
3. Alkaloid momordicasoides gives the bitter taste of the fruit.
4. Fruit is rich in P (55mg/100g), Ca (20mg/100g) and vitamin A (210IU/100g).
5. Fruit is wormicidal and cure stomach disorders and has beneficial effect on the
persons suffering from diabetes arthritis and asthmatic complaints.
6. Powder prepared from plants is useful against ulcers.
49
7. Protein of bitter gourd inhibit the growth of HIV 1 viruses in human cell cultures
Taxonomy
The genus Momordica has 45 species domesticated in Asia and Africa. The genus
Momordica has only six valid species in India, which can be grouped under two headings.
Monecious
M.charantia L.
M.balsamina L.
Dioecious
M.dioica
M.cochinchinensis
M. foetida
Indian bitter gourd is classified into two botanical varieties based on fruits, size,
shape, colour, and surface texture:
M.charantia var.charantia has large fusiform fruits,which do not taper at both ends
and posses numerous triangular tubercles giving the appearance of crocodiles back.
M.charantia var.muricata(wild) which develops small and round fruits with tubercles,
more or less tapering at ends.
M.dioica
50
M. foetida
Momordica cochinchinensis
51
Momordica balsamina
M. charantia is propagated through seeds and is monoecious. M. dioica and M.
cochinchinensis are propagated through underground tubers and are dioecious in nature.
Both species have small fruits covered with spines. Main differences between the two
species are as follows,

52
Kakrol or sweet gourd Kartoli or spine gourd
(Momordica cochinchinensis Roxb) (Momordica dioica Roxb)
Roots develop bigger tuber Roots develop small tuber.
Leaves are bigger. Leavers are small.
Flowers large and white to light yellow in Flowers small and yellow in colour.
colour.
There are three small circular dots at the base of No circular dot on the base of petals.
petals which are deep blue.
Anthesis during early morning (3.30-6.30 hours) Anthesis during evening (16.30-18.00
and flowers take 72 hours to open. hours) and flowers take 7-22 minutes to
open.
Fruits are large and oblong Fruits are small and round to oval.
Individual fruit weight is around 60-80g and Individual fruit weight is around 10-15 g
attains upto 500g. and attains upto 30g.
Fruit ripening starts from periphery to inner Fruit ripening starts from inner to
periphery.
Fruit light green to light yellow in colour Fruits dark green in colour.
Tough spines on fruit. Smooth and false spines on fruit.
It takes 26 days to reach edible maturity from It takes 20 days to reach edible maturity
days to bud formation from days to bud formation.
Short flowering and fruiting period Flowering and fruiting continue for long
period.
53
CYTOGENETICS
 Chromosome number is 2n=22, x=11
 Tetreploid lines with 2n=44, were obtained by treatment by seedlings with 0.2
% colchicines for 18 hr to the shoot tips and triploids by crossing 4x with 2x
plants.
 The natural triploid (2n=33) of the cultivated M.charantia was reported from
India.
Floral Biology
Bitter gourd is highly cross pollinated. Flowers are auxiliary with long pedicel and are
yellow in colour. Stamens are 5 in number with free filaments and united anthers.
Stigma is divided
Anthesis and Pollination:
The anthesis starts from 4am and extends till 9am.
Anthers dehisce from 5am to 7am.
Stigma is receptive 24hr before and extends for a day even after anthesis.
54
Female flower Male flower
BREEDING OBJECTIVES
1. Early fruiting.
2. High female to male sex ratio.
3. Whitish green to glossy green fruit colour depending upon consumer‘s
preference.
4. Less ridged fruit surface.
5. Immature seeds for longer period during edible stage.
6. Higher yield (number of fruit weight). Fruit sizevariation as per consumer‘s
preference (small 7.5-10cm long, medium long 10-15cm long 15-20cm and
extra long 20-40cm).
7. Resistance to red pumpkin beetle, fruit fly, downy mildew and mosaic virus.
55
BREEDING METHOD
Bitter gourd is highly cross pollinated in nature due to pre dominantly monoecious
sex form and entomophilous nature.
Selection
Single plant selection and mass selection methods were followed to develop high
yielding lines of bitter gourd in India.
Pusa Mousami, Priyanka, Konkan Tara, Arka Harit and Pusa Vishesh.
Heterosis Breeding
Heterosis in bitter gourd was investigated at the Indian Agricultural Research

Institute, New Delhi,
Pusa hybrid-1
RHR BGH-1
Mutation Breeding
One such bitter gourd cultivar, MDU 1, developed as a result of gamma radiation
(seed treatment) of the landrace cultivar MC 103, was found to posses improved
yield.
Hybridization: Major varieties developed through hybridization are
Pusa hybrid-1 : Pusa Do Mausami × Pusa Vishesh
Pusa hybrid-2 : S-63 × Pusa Do Mausami.
Urvasi : IC-85650B × IC-4435
Phule Green Gold : Green long × Delhi Local
BTH-7 : VRBT-3A × VBRT-82-1

BTH-165 : KB-1 × VRBT-36
Polyploidy Breeding:
Tetraploidy was induced by colchicines treatment. Soaked seeds(24hr) and sprouted

seeds were treated separately with 0.1 and 0.3 % colchicines for 6,12 and 24 hr.
Soaked seed treatment for 24 hr colchicines were effective.
Intergeneric hybrization:
56
M.charantia with Trichosanthes anguina was crossed. Over 50% of the pollinated
flowers developed into fruit. Of the seeds, 50% germinated well, producing a normal
crop. The floral and vegetative characters of M.charantia were dominated.
Resistance Varieties for biotic and Abiotic stress
Varieties Features
Kalayanpur Barahmasi Tolerant to fruit fly and mosaic virus
Phule BG4 Resistance to fruit fly
Kalyanpur Sona Tolerant to mosaic virus and fruit fly
Phule Green Gold Tolerant to downy mildew
BG 98 and BG 102 Resistant to red pumpkin beetle
IC 68275 BDMV Bitter gourd distortion mosaic

virus resistant
Pant karela-1 Highly resistant to red pumpkin beetle
BTH-7 Field tolerant to powdery mildew
Green rough, Green smooth,White Resistant to fruit fly

Rough and White smooth
BTH-165 Tolerant to powdery and downy mildew

57
VARIETIES AND HYBRIDS
Consumer preferences in bitter gourd vary from region to region depending on size,
colour, presence or absence of tubercles / ridges and bitterness of fruits. Accordingly a
number of varieties are developed in India and details are furnished below:
Breeding
Variety Organization Characteristics
method
Pusa Do Mousmi IARI, New Delhi Selection This variety has been
obtained through
selection from local
germplasm at IARI and
released by the same
organization. It is
suitable for both the
spring –summer and
rainy season. The fruit
are dark green long
(18cm), medium thick,
somewhat club shaped,
and 7-8 continuous ribs.
Fruit weight 100-120 g.
Yield 12-15 t/ha.
Pusa Hybrid 1 IARI, New Delhi Hybridization Fruits medium thick,
long and gloss green,
yield 20 t/ha in 120 days
Pusa Vishesh IARI, New Delhi Selection from a suitable for growing
local collection during summer. Fruits
glossy green medium
long and thick.
Priya (VK1)* KAU, Selection This variety came as a
Vellanikkara result of selection at
KAU,Vellanikkara in
the local germplasm. It
is characterized by
extra-long fruits (about
50 fruits/plant) Extra
long green spiny fruits
with white tinge at stylar
end, av. Fruit length 39
58
cm. av. Fruit weight 235

g. productivity 24.5 t ha -
1
Preethi( MC 4)* KAU, Selection High yielding variety

Vellanikkara released from the
KAU,Vellanikkara.
Medium sized greenish
white fruit, with spines,
av. Fruit length 30 cm,
av. Fruit girth 24 cm, av.
Fruit weight 0.31 kg.
Average yield is 10-
35t/ha. Relatively less
susceptible to fruit fly
attack. Suitable for
growing in
Thrisur,Palakkad and
Ernakulam districts.
Priyanka Large white spindle

shaped fruits with
smooth spines, thick
flesh and less seeds. Av.
Fruit length 25 cm. av.
Fruit girth 20 cm. av.
Fruit weight 0.30 kg.
Productivity 28.0 t/ha-1
Arka Harit IIHR, Bangalore Selection The fruit are short,
spindle shaped,
attractive, glossy green
with smooth regular ribs
and thick flesh with
moderate bitterness.
Yield 9-12 t/ha.
Pant Karela 1 GBPUAT, Selection This is a selection from

Pantnagar inbreds of indigenous
germplasm at
Pantnagar released in
59
1999 by UP State
variety release
committee. Fruits are
thick 15cm long, Yield
is about 150q/ha.
Coimbatore long TNAU, Selection It is a local type selected

Green Coimbatore at TNAU, Coimbatore.
Fruit are extra long, up
to 60cm, dark green
weighing 300-400g
yield potential is
180q/ha
CO.1 TNAU, Fruits dark green with

Coimbatore medium length (20-25
cm) and weight (100-
120 g). Yield 14 t/ha.
MDU.1 Tamil Nadu Fruit weight 300-450 g.
Agricultural yield 15-18 t/ha.
University Induced mutant with
long (30-40 cm)
greenish white fruits,
fruit length 30-40 cm,
yield 30-35 t/ha.
Coimbatore Long TNAU, Extra long fruits (60-65
White Coimbatore cm) with white colour,
yield 15 t/ha.
COBgH 1 TNAU, Hybridization F1 hybrid developed by
Coimbatore crossing MC 84 x
MDU1. Fruits are light
green in colour, plumpy
with more warts, each
weighs 200g.-300g.
Yields 44.40 t/ha in 115-
120 days. It is rich in
momordicin (2.99 mg
per 100g).
60
Phule Green Gold MPKV, Rahuri Hybridisation It has been developed by

pedigree method from a
cross of Green long ×
Delhi Local at MPKV,
Rahuri. Fruit is dark
green, 25-30cm long and
prickled. yield potential
is about 200q/ha in 150-
180 days, tolerant to
downy mildew.
Phule Priyanka MPKV, Rahuri Hybridisation Hybrid variety with dark

coloured fruits. Fruits
are 20-25cm long with
tubercles.
Phule Ujwala MPKV, Rahuri Selection Fruits 18-20cm long,

dark green in colour
with a few white
tubercles, average yield
30-35t/ha.
Hirkani MPKV, Rahuri. Fruits dark green, 15-20

cm long, spindle shaped
with warts and prickles,
yield 14 t/ha in 160
days.
Konkan Tara Konkan Krishi . Fruits green, prickly,
Vidya Peeth, medium long (15-16 cm)
Dapoli and spindle shaped.
Yield 24 t/ha
Punjab 14 Punjab Plants bushy and bear
Agricultural light green fruits with
University, average weight of 35 g.
Ludhiana Yield 14 t/ha.
Kalyanpur CSAUA&T. Fruits long (30-35 cm),
Baramasi Kanpur light green, thin and
tapering, tolerant to fruit
fly and mosaic, yield 20
t/ha in 120 days.
61
Arka Harit MDU1
Preeti
Pusa Do Mausami Pusa Vishesh

62
Co1
CoBgH1
Questions:
1.What are all the dioecius species of bittergurd?
2. Name some varietes released from TNAU
3. What are the breeding objectives in bittergurd?
4. What is the mode of pollination?
5. Name some F1 hybrids of bittergourd
References
Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of Vegetables, Revised,
ICAR, New Delhi.
Agency, New Delhi.
Agency. New Delhi.
63
RIDGE GOURD
L-9
Ridge Gourd (Luffa acutangula Roxb.)) (Hindi : Ghia tori)
Smooth Gourd (Luffa cylindrica Roem.) (Hindi: Kali tori)
Cucurbitaceae
CHROMOSOME NO : 2n=26
Ridge gourd can be grown throughout the year and are used as vegetables. It is more
popular vegetables in the south and east India.
ORIGIN: India
wild species
Luffa graveolens
Luffa echinata
 The wild species, Luffa graveolens is progenetor of cultivated species
Luffa acutangula
64
Luffa cylindrica
Importance of the crop
 Both ridge or ribbed gourd and smooth or sponge gourd contains a gelatinous
compound called luffein. It has lot of medicinal uses.
 Ridge gourd is very important for those who suffering from diabetes and
hypoglycemia.
 Ridge gourd is also a good anti-inflammatory agent thus helping with cardiovascular
disease, strokes, and cancer.
 The oils from the seeds are known to cure coetaneous complaints, the roots have
laxative effects.
 The juice from the leaves is used to cure granular conjunctivitis of the eye, adrenal
type diabetes and hemorrhoids.
 Genus name was derived from the product ―Loofah‖ used as bathing sponges,
scrubber pads, doormats, pillows, mattresses, cleaning utensils, etc.
Floral Morphology
 The sex form in ridge gourd is monoecious, gynomonoecious, andromonoecious and

hermaphrodite.
 Male inflorescence in raceme of several flowers.
 Female flower solitary, borne axillary as male flowers.
 The flowers are pale yellow to yellow in color.

65
 Calyx – 5 lobed, united at the base .
 Corolla – 5 petals, companulate, bright yellow in colour .
 Androecium – stamens are three attached to the calyx tube, anthers free.
 Gynoecium – ovary is inferior, three carpellary with one or three called with 3 parietal
placements.
Floral Biology
 ANTHESIS :
 Anthesis starts in the evening by 5.00 p.m. and continues uptop 8.00 p.m
 POLLINATION : cross pollination .
 ANTHER DEHISCENCE
 The anther dehiscence is seen immediately after anthesis.
 Pollens are fertile from the time of dehiscence till 2 to 3 days in winter and 1 to 5 days
in rainy season.
 The stigma is found to be receptive even 6 hrs before anthesis and continues to be
receptive till 84 hrs after anthesis.
Male flower Female flower
Breeding objectives
 Earlyness
 High fruit yield ( Arka swathi gives high yield 52t/ ha )
 Earlyness
 Uniform thick cylindrical fruits.
 Tender , non fibre fruits for longer lime.( eg for tender : Punjab Sadabahar)
66
 Resistance to diseases like powdery mildew (eg.Hisar Kalitori) , downey mildew ,

virus (eg. Gujarat Anand ridge gourd-1).
 Resistance to insects like red pumpkin beetle.
 Resistance to biotic and abiotic stress.
 FIBRE TYPE :
 L. aegyptiaca may be allowed to mature and use as a bath or kitchen sponge after
being processed to remove everything but the network of xylem fibers.
 BITTER TYPE : eg . Luffa amara
 NON BITTER TYPE: Eg. Pusa Nutan and other cultivable varieties.
Fibre type
Bitter type
Breeding objectives
Single plant selection :, co-1, co-2
 pedigree method
67
Useful to improve highly heritable characters interspecific hybridization.
Luffa actutangula × L.cylindrica
Luffa actutangula × L. graveolens
Luffa echinata × L. graveolens
Polyploidy breeding
 Colchicine induced tetra ploids of Taiwan variety to exhibited thicker and shorter
fruits lower fertility than corresponding dipliods.
 Breeding for resistance to diseases in heritance of ridge gourd resistant to downy

mildew using dialleles crosses among 6 cultivars.
 Hybridization
Major varieties developed through hybridization are
Arka Swathi : cross between Medium fruits at IIHR-54 × long fruit at IIHR- 18
Arka Sumeeth : cross between Early fruits at IIHR-54 × long fruit at IIHR-18
Maintanence of inbred lines
 Uniform inbreds of luffa could be developed with comparable vigor of open

pollinated varieties.
 They can be maintained through open pollination by growing them at proper isolation
distance.
 Isolation distance of 1000 m is quite appropriate.
 Inbreds can also be maintained by hand pollination.
Genetics of sex expression

Form Genotype Behavior on selfing
Monoecious AAGG Monoecious
Gynoecious A-gg Unstable and do not
breed true
Androecious aaGG
Andromonoecious a‘ a‘ GG Andromonoecious
Gynomonoecious AA g‘ g‘
Hermaphrodite aagg Hemaphrodite
a‘a‘ gg Hermaphrodite
aag‘ g‘ Hermaphrodite
a‘ a‘ g‘ g‘ Hermaphrodite
68
Varieties
Varieties Organization Breeding Features

methods
Pusa Nasdar IARI, New Delhi Selection Midseason,flowering in 50

days,15-20 fruits/vine, fruits
ridged,light green. More
suited for rainy season.
Pusa Nutan IARI, New Delhi selection Fruits are long (25-30cm),
straight attractive green,
average fruit wt.105g, flesh
tender
Hisar Kalitori HAU,Hisar, Selection Early long thin and straight
Haryana fruited to Powdery mildew,
suitable for rainfed areas.
GJRGH-1 – GAU, Gujarat
The fruits of GJRGH-1 are long in size with green colour.
Suitable for kharif season
Gujarat Anand Ridge gourd -1
AAU, ANAND, GUJARAT
This variety is medium sized and elliptical shape with green fruit skin colour. The
variety has less mosaic virus and downy mildew disease reaction.
Pant Toria-1
GBUA &T, Pantnagar
The main shoot is 5m long. Fruits are 15-20 cm long and club shaped. It takes
about 65 days to first harvest.
It is specifically suitable for rainy season.
CO1
TNAU, Coimbatore
Fruits are long (60-75cm) light green and weight 300 g each. Yield 14t/ha.
CO-2
Fruits are very long(1m) green & fleshy. Yield 28 t/ha.

69
Satputia
RAU Sobour Bihar
Vine produce bisexual flowers instead of male and female flowers separately.
Fruit borne in clusters.
Arka swathi
IIHR, Banglore
Cross between medium fruits at IIHR-54 and long fruited IIHR. Yield 50t/ha.
Arka sumeeth
 IIHR, Banglore
 It is a cross between early fruiting IIHR- 54 and long fruited IIHR.
 yield 50t/ha.
Punjab Sadabahar
 PAU, Ludhiana
 This varieties are medium sized with dark green leaves. The fruit is 3-5 cm long
thick,smooth green ridged, tender, slightly curved and rich in protein.
Pusa Nutan Gujarat Anand

70
CO -1 Satputia
GJRGH-1 Arka sumeet
Pusa Nasdar Pant Toria-1
Swarna Sawani(satputia 2013,IVRC)
 Pureline selection
 Plant vigorous with 3-4m vine length
 Early flowering and fruiting

71
 Suitable for rainy seasons
Swarna Uphar (2006,Gazette No.1422)
 Pedigree method
 Early flowering and fruiting
Arka Prasanth (IIHR)
 open pollinated ridge gourd
 Release by Institute VTIC during 2016
 Early flowering & first female flowers appears at 9th node from the base of the vine.
Arka Vikram F1 hybrid
 Early flowering & first female flower appear.
Swarana Sawani Swarna Uphar
Arka Prasanth Arka Vikram
Varieties – Sponge gourd

72
Variety Special features

Developing
institution
IARI, New Pusa Early variety. Fruits smooth dark green and cylindrical,
Delhi. Chikni 15-20 fruits / plant.
Pusa Fruits pale green, 15-20 cm long, straight and slightly
Supriya curved at stem end, pointed distal end with long peduncle,
average fruit weight 110 g at vegetable harvest stage.
Yield 10-11 t/ha.
Pusa Sneha Suitable for long distance transport, fruits dark green, 20-
25 cm long with hard skin and soft flesh. Yield 12 t/ha.
MPAU, Rahuri. Phule Fruits medium green with dark green sutures, yield 15
Prajakta t/ha.
BAC, RAU, Rajendra Fruits long, greenish white, smooth and thick, resistant to
Sabour, Bihar Nenua 1 fruit fly and fruit rot. Yield 25 t/ha.
Questions :
1.Ridge gourd varieties released from IIHR
2. What is the origin of Ridgegourd?
3. What is the mode of pollination ?
4. Ridgegourd variety released from TNAU
5. Name some hybrids of Ridgegourd.
References
73
PUMPKIN
L-10
 BOTANICAL NAME : Cucurbita moschata
 FAMILY : Cucurbitaceae
 CHROMOSOME NO : 2n=40
 ORIGIN: : South America
 FRUIT TYPE: : Pepo
Species
 C.moschata - Pumpkin
 C.pepo – Summer Squash
 C.maxima – Winter Squash
 C.ficifolia – Fig leaf gourd
 C.mixta
 C.foetidissima – Buffalo gourd
Diversity in size shape and colour:
 Mature and immatre fruit: vegetables,
 Large seed (Pumpkin nuts or pepitas)-rich in Zn,
 Seed : De - worming treatment,
 Nutritious,
 Diuretic.
74
 Leaves: Pain killer.
Seeds
Botany
 Monoceous, annual Herb, viny growth, Runner.
 Hairy trichomes, spring like long, branched tendrils.
 Leaves: Five-loabed, long petiole, alternately arranged on the stem.
 Flowers: Large, showy, yellow or creamy corolla.
Plant Flower
 Male flower: Near the centre of plant & long slender pedicels
 Female flower: Short, slender pedicels, Calyx and corolla companulate.
 3 Anther , thick style, stigma are three each are two loabed.
 FRUIT: Pepo
Floral biology:
 Anthesis, Stigma receptivity,
 Pollen dehiscence at early morning.

75
 Stigma receptivity til mid day.
 Flower open at temperature above 50o F.
Breeding objectives
 High yield
 Earliness, high female to male ratio.
 Brta carotine (yellow) rich,
 Non ridged rind, Thick flesh and narrow seed cavity
 First pistilate flower at early node numbers,
 High Anti-oxidents specially carotinoids.
 Rich in vitamin – A
 High Anti-oxidents specially carotinoids
 Tolerence to low temperature & saline,
 Resistance to pest and disease
Genes responsible for various attributes
 Deep colour fruit with vitamin – A(B gene)
 Mottled light and dark green fruit colour(mldg)
 Herbicide tolerence (T)
 Silver leaf mottling (m)
 Yellow mosaic virus (zym)
 Fruit fly resistance in C.maxima ‗Arka suryamukhi‘ (Fr)
 Gynoceous sex expression on sp – foetidissima (G)
 Green rind from ‗Long Neopotitan‘ (Gr)
 Hard rind – in ornamental gourds(Hr)
 Powdery mildew resistance from C.lundelliana (Pm)
 Umbrella like leaves (uml)
Breeding Methods
76
 INBREEDING:
 Inbreeding along with selfing.
 No inbreeding depression in cucurbita sp
 Even after prolonged selfing.
 Inbreeding applicable for all cucurbits
Hybrid breeding
 1.MANNUAL POLLINATION:
 Maintain several female rows alternate with one male row.
 Male flowers detected and removed from female rows before anthesis.
 Female flowers in female parent row are hand pollinated by male flower of male
parent row.
 USE OF CHEMICALS:
 2-chloroethyl phosponic acid(Ethephon)-
 Sex regulator( suppression of male flower)
 Spray at 2 and 4 leaf stage at 600 ppm is completely supprese male flower in plant.
Interspecific Hybridization
F1 hybrid between 2 sp difficult because such hybrid are sterile.
C. maxima x C. moschata found to be fertile and produce fruit.
MMMM x PPPP-------- MMPP x MM------------- MMP x MM --------- MM P
M=C. moschata
p=C. maxima
SELECTION:
Punjab Chappan Kaddu 1(Inbreeding selection)
Arka Chandan-collection from Rajasthan.
High beta-carotene content.(C.moschata)
Arka Suryamukhi-selection from forign introduction, Resistant to fruit fly(C.maxima)
Pusa Bishwas- selection of line SM-107

77
(C.moschata).
Hybrids
 Pusa alankar- EC-27050 x Sel.IPI-8
 Pusa hybrid – 1: Sel-107 x Sel -124
 transgenic varitiey:
Freedom: Resistant to Zucchini virus
Varieties
CO 1
It was evolved at Tamil Nadu Agricultural University, Coimbatore. A local selection,
with late maturing fruits, medium large size with a characteristic broad proximal end. The
distal end will be slightly tapering to form a tip. Immature fruits are dark green in colour.
After full maturity, they become brownish orange. Each fruit weighs on an average 7-8 kg. It
yields 30 t/ ha in a crop duration 150-160 days.
CO 2
Another selection from a local type with small flat fruits weighing 1.5-2.0 kg each. It
yields about 23-25 tonnes/ha. in a crop duration of 135 days. Suitable for kitchen garden and
family use.
Arka Chandan
It was developed at Indian Institute of Horticultural research, Hessarghatta.
A selection from Rajasthan Collection. Fruits are medium in size each weighing 2.3 kg with
depressed polar ends. Rind colour is light brown with creamy patches at maturity. The crop
duration is 125 days.
Ambili
78
This variety was developed at the College of Horticulture, Kerala Agricultural
University, Vellanikara. It is a pure line selection made from a local cultivar of Thrissur. It is
a vigorously growing spreading variety having flat-round fruits of medium size with shallow
grooves. The fruits are green in colour in the immature stage. Leaves are characterized by
white spots on the upper surface of the lamina. Each fruit weighs on an average of 5-6 kg.
It has got an yield potential of 34 t/ ha.
Solan Badami
It was developed at University of the Horticulture and Forestry, Solan, Himachal
Pradesh. It is a small-fruited variety with a yield potential of 22.5 t/ ha.
Pusa Biswas
A local selection of the line CM 107 at IARI, New Delhi. It has vigorous growth,
darkgreen leaves with white spots including veins. Fruits are light brown with thick golden
yellow flesh and are spherical. Average weight is 5 kg. It matures in 120 days. The potential
yield 20 t/ ha.
Pusa Vikas
It was developed at IARI, New Delhi. It is a small-fruited semi-dwarf to dwarf type.
The fruits are small, round and flat weighing on an average 2.0 kg each, fruit flesh being
yellow. Highly suitable for cultivation in spring-summer season in North India. Its potential
yield is
30 t/ ha.
Cucurbita pepo
Early Yellow Prolific

79
It is a bush type with fruits medium in size and tapering towards the stem end;
skin orange yellow at maturity. It was released by IARI Regional Station at Katrain. Duration
80-90 days.
Australian Green
Fruits are dark green with longitudinal white stripes; 25-30 cm long, very tender at
edible stage. It was also released by IARI Regional Station, Katrain.
Patty Pan
An introduction from USA with disc shaped chalky white fruits, duration 85 days.
Released by IIHR, Hessarghatta, Bangalore.
Punjab Chappan Kaddu
It is an inbred selection from segregating local material of Punjab, developed at
Punjab Agricultural University, Ludhiana. Plants are bush type, foliage thick and erect.
Leaves non-lobed and green without white specks. Fruits green, disc shaped. Average fruit
weight 800 g and a plant bears about 10 fruits. It has a potential yield of 20-22 t/ ha.
Pusa Alankar
It is an F1 hybrid of EC 27050 x Sel-1-PI.8 developed at IARI, Regional Station, Katrain. It is
an early maturing (50 days) variety with uniform dark green skin with light coloured stripes.
Fruits round with slightly tapering towards stem end.

80
Cucurbita pepo( Spoon gourd) Cucurbita pepo( Summer squash)
Cucurbita maxima
Arka Suryamukhi
It is a selection made at IIHR, Hessarghatta from a foreign introduction. Fruits are
small (1 kg) round with flat ends and deep orange skin and pale white stripes just like rays of
sun; flesh firm, orange in colour and with pleasant flavour; Duration 100 days. This variety is
resistant to fruit fly (Daucus cucurbitae).
Cucurbita maxima( winter squash)

81
Questions:
1.What are all the related species of Pumkin?
2. Name some varietes released from TNAU
3. What are the breeding objectives in Pumkin?
5. Name some F1 hybrids of Pumkin
References
ICAR, New Delhi.
Agency, New Delhi.
Agency. New Delhi.
82
Ash gourd
L-11
(Hairy melon, winter melon, ash pumpkin, white pumpkin, wax gourd, white gourd,
petha)
Scientific Name : Benincasa hispida
Chromosome No : 2n = 24
Origin:
Ash gourd probably originated from India.
Distribution:
Southeast Asia, East Asia and South Asia
 Ash gourd is cultivated for its immature as well as mature fruits which are used as a
cooked vegetable and are used in confectionary and ayurvedic medicinal
preparations.
 The delicacy ‗Petha‘ made out of ash gourd is famous all over India.
 A small fruited medicinal ash gourd is also grown in Kerala.
 The famous ayurvedic preparation ‗Kooshmanda rasayana‘ is made of ash gourd
fruits.
 Ash gourd is good for people suffering from nervousness.
Botanical Description:
Habit: Robust, annual, usually monoecious, climbing herb up to several metres long.
Plant: Ash gourd is a crawling or climbing vine. The vines can be several meters long.
Leaves: Alternate, simple, reniform - rounded, 10-25 cm long and as broad, deeply cordate,
upper surface scabrous, lower.
83
Root: Tap root
Stem: Branched, diffuse, having slender tendril.
Stem Anatomy of white gourd:
Inflorescence: Plants monoecious, male flowers solitary, pedicels 5-15 cm long, densely
hispid and villous. Bracts ovate or broadly oblong, 6-15 × 5-10 mm, apex acute.
Calyx: Tubular 12 -15 mm in diameter, densely villose, lobes lanceolate, 8-12 × 3-5 mm.
Corolla: Yellow, lobes 3-6 × 2.5-3.5 cm, both surfaces pubescent.
Androecium: Stamens 3, free, inserted at the calyx tube, filament hispid, 2-3 mm long, base
expanded, anthers 4 mm long, base expanded, anthers 4 mm long, sub- trilobite.
Flowers: Solitary in leaf axils, large, 8-12 cm in diameter, yellow, unisexual, pentamerous,
pedicel densely hispid
Male flowers: 5-12 cm long, 5 stamens, 4 of these in connate pairs.
Female flowers: 3-4 cm long, cylindrical ovary and short style with three curved stigmas.
Fruit: Pepo, the outer layer covered with chalk white easily removable layer of wax, flesh 2-4
cm thick, white, succulent, slightly fragrant, spongy in the middle.
Seeds: Numerous, flat, ovate-elliptic, white, often with marginal rigid.
Floral biology
• Cross pollinated due to monoecious condition
• Male : Female 34:1
• Anthesis – 4.30 – 7.30 am anther dehiscence 3-5 am
• Stigma receptivity – 8 hours before anthesis to 18 hours after anthesis

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• Pollen fertility – 95% at anthesis
MALE AND FEMALE FLOWER
Related Species and Genera:
Benincasa cerifera Savi
B . cylindrica Ser.
B. hispida var .chieh –qua
B.hispida var. hispita
B. pruriens (Parkinson)
B. vacua
Cucurbita hispida Thunb.
Gene symbol:
Sex controlled by two genes.
A-G : Monoecious
A-gg : Gynomonoecious
Aa G : Andromonoecious
Aagg : Hermaphrodite
Ar-1 : Anthracnose resistance to race 1
85
f : Farrowed fruit surface, recessive to smooth

Fo-1 : Dominant gene for resistance to race 1 of Fusarium oxysporum
sp. Niveum.
Fwr : Resistance to fruit fly dominant gene
Pm : Powdery mildew susceptibility
Ag : Tolerant to aphids.
Breeding objective:
1) Earliness
2) High female to male sex ratio.
3) High fruit yield.
4) Resistance to drought and salt stress.
5) Resistance to common diseases like powdery mildew, downy mildew and virus and
the insects like red pumpkin beetle.
6) Tough skinned fruits for long transportation
7) Development of seedless ash gourd.
Breeding Methods:
• Cross pollinated
• Mass selection
• Pedigree method
• Bulk population method
Selection:
• Ash gourd being a highly cross pollinated crop has a high degree of heterozygosity.
• Therefore selfing followed by selection improves the population and helps to develop
a new variety with district feature from the original.
• Different selection methods can be applied for population improved.
• CO 1 & CO 2 developed through selection
Mass Selection:
• The selection of superior plants form the base population and missing of their seeds
for raising the next generation are followed in this method.
• This selection procedure is repeated in uniform growing conditions up to the selection

as a new variety / type.
86
• This method is effective in improving simply inherited and highly heritable

qualitative characters.
Single Plant Selection:
• Single plant selection is a very common method of selection.
• The selfed individually in the next generation for evaluation and maintained by
selfing.
• The advanced progenies do not show loss of vigour due to inbreeding.
• Therefore, homozygosity for the concerned, the best can be attained in the individuals
of the progeny by selfing.
• After necessary evaluation, the best selection can be treated as a new type.
• Several varieties have been developed by single plant selection is ash gourd.
Inbreeding and Selection:
• Inbreeding is the mating of closely related individuals, that is either selfing or sib
mating.
• Individual selection of practiced after attaining maximum uniformity and claimed as a

variety.
Hybridization:
• Hybridization creates new genetic variability in the F2 and subsequent generations

that helps efficient selection of desirable types.
• The hybrid progenies are advanced in the subsequent generations by selfing to

selected desired types.
• Single plant selection by maintaining the pedigree of segregating generation is

applied.
Backcross Breeding:
• Backcross breeding consist of crossing of F1 with one of the parents (Recurrent

parent) followed by selection of genotypes for specific characters.
• One to three backcross may be made till homozygosity is attained. This method is
generally applied to transfer simple inherited characters like resistant and some
morphological traits to the unimproved variety.
87
• Two types of parents are involved in this breeding method: One as a recurrent parent
(high yielding) and the other as a donor parent (Low yield but processing specific
desirable traits).
• This method is commonly used for the development of resistant varieties.
Heterosis Breeding:
• The heterosis for the number of fruits and total yield were found to be 46% and 26%,
respectively.
• High yield of F1 hybrid was contributed by various traits such as early maturity,
longer vine, more fruits per plant, bigger fruit size and higher fruit weight.
• In most of the crosses, all the characters were under the control of duplicated epistasis
indicating high promise for heterosis breeding in ash gourd.
• It is a highly cross- pollinated crop therefore it is necessary to produce inbred lines.
• If varieties / lines are maintained as pure lines by inbreeding, these lines can be used
as inbred lines.
• These inbred lines will be crossed in different mating designs (diallel, line × tester) to
test the specific and general combining abilities of lines.
• Once a good heterosis parental combination has been identified, the hybrid seed can
be easily produced by adopting any one of the following methods.
1) Pinching of staminate flowers before anthesis and head pollination where isolation
distance is not available.
2) Pinching of staminate flowers before anthesis on pistillate parent and insect
pollination where isolation distance is available.
3) Chemical suppression of staminate flowers and insect pollination (at proper isolation).
Mutation Breeding:
 Generally mutations are heterozygous and recessive. Therefore, the mutant
phenotypes are not expressed in the M generation.
 Several chemical mutagens (ethyl methane sulfonate, diethyl sulphate ethyl amine
etc.) may be used for inducing the mutation.
 Besides, physical mutagens such as X- rays, gamma rays and neutrons also may be
used for induction of mutation.
 In ash gourd the development of seedless fruits may be one of the mutation breeding.
Breeding Achievements:
Variability in ash gourd is limited except for size and shape of fruits. The improved varieties
are:
TNAU Ash gourd hybrid Co 1:

88
 It is a F1 hybrid between PAG 3 × CO 2.

 Plants are medium viny.
 Fruits are oblong and medium sized (4-5 kg) in a duration of 120-130 days and
suitable for small families.
 High yielding with an average yield of (91 -82 t/ ha).
CO1:
 Developed by TNAU, fruits are oblong oval shaped.
 This is a local selection variety of Tamilnadu, each fruit weighing 8 to 10 kg with
crop duration 140 to 150 days.
 Yield about 50 to 60 kgs per acre.
CO 2:
 Selection from Coimbatore local variety.
 Fruits are oblong in shape.
 Each fruit weights about 2 to 4 kg with crop duration 120 to 130 days. Yield about 84
tons per acre.
INDU AG 1:
 Round shaped fruits with high flesh thickness, length 24 cm weighing about 4 to 5 kg
each.
 This variety is tolerant to mosaic virus diseases.
Ekalavya:
 Developed by innovative farmer, Shri A.S. Joy of Thrissur, Kerala as a Hybrid.
 This is a Mosaic resistant variety.
 Fruits weigh 12 Kg. Crop duration 140 days. Yield 250 tons per acre.
KAU Local:
 Developed by Kerala Agricultural University, Thrissur, Kerala, Oval shaped fruits
having high flesh thickness, length 45 to 55 cm and weight 6 to 8 kg.
 Yield about 70 tons per acre.
 This variety is tolerant to mosaic diseases.
Kashi Surbhi:
 Fruits oblong, ellipsoid, rind greenish white, flesh white, average fruit weight 10 -12
kg.
 Fruits are suitable for long distance transportation.
 It has yield potential of 600 -700 q / ha.
Pusa Ujawal:
 Developed by IARI, New Delhi.
89
 This is resistance to viral Disease.

 Average yield 40 ton / ha during summer and 43 ton / ha during Khariff season.
Kashi Dhawal (IVAG 502):

 Derived from a local collection, vine length 7.5 -8 m, fruit oblong, flesh white,
thickness 8.5 -8.7 cm, seed arrangement linear, average weight 11-12 kg, crop duration
120 days, yield 550 -600 q / ha.
Kashi Ujawal (IVAG 90):

 Derived from local collection, vine length 7.5 -8 m, fruits round with average weight
of 10 -12 kg fruit flesh white with 7 cm thickness, seed arrangement linear, crop
duration 110 -120 days, yield 55-60 t /ha.
Kashi Surbhi Kashi Dhawal
Kashi Ujwal TNAU Ash gourd Hybrid CO 1

90
KAU Local
Some other varieties of ashgourd
Developing Variety Special features
Institution
APAU, Hyderabad. APAU Shakthi Fruits long and cylindrical, yield 30-35 t/ha in 140-150
days.
UAS, Bangalore. ―Karikumbala‖ Local cultivar where the fruits are covered with ashy
coat.
In addition to above open pollinated varieties, a few F1 hybrids like MAH 1; MHAG
2 etc. are developed under private sector in the country.
Questions :
1.Ashgourd varieties released from IIHR
2. What is the origin of Ashgourd?
4. Ashgourd variety released from TNAU
5. Name some hybrids of Ashgourd.
References
91
WATERMELON
L-12
 Scientific name : Citrullus lanatus
 Family: Cucurbitaceae
 Chromosome No: 2n=2x=22
 Origin :
Primary: Southern Africa
Secondary: China (related species found in India)
 Grown in Middle East, the United States of America, Africa, India, Japan and Europe.
OTHER SPECIES
 Citrullus edulis 2n = 22, 24
 C. naudinus n = 22
 C. colocynthes 2n = 22
 C. fistulosus
 Types : seeded, seedless
 Sizes : ice-box, small, medium, large, giant
 Shapes : round, oval, blocky, elongate
 Rind patterns: gray, narrow stripes, medium stripes, wide stripes, light solid, dark
solid.
 Flesh colours : white, yellow, orange, red
Variations in flesh colour

92
BOTANY
 Plants are viny type, monoecious, with angular stem.
 Leaves are pinnately divided into 3-4 pairs of lobes
 COROLLA: rotate, five lobed, sulphur yellow or chrome yellow.
 cross pollinated crop
 Fruit sizes varies from 1.5kg to 50kg(10-12kg common)
 The greater part of fruit flesh is mostly derived from placentae.
 Edible part : mesocarp.
 Flower : less showy, monoecious cultivars. Pistillate or hermaphrodite flowers occur

every seventh leaf axil intervening axil are staminate flowers.
 Three stamens are at the base of the corolla.
 Andromonoecious
 Controlled and open pollination
FLOWERS
 Anthesis : 5.30 to 6.30 am
 Fruit setting: watermelon does not have flowering peaks or fruiting cycle there is an
inhibitory influenced produced by fruit already set that reduces further fruit setting.
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Important genes :
 a - andromonoecious, recessive to monoceious.
 Ar1-, Ar2- - Anthracnose resistance, resistance to Giomerella cingulata var. orbiculare
 C - canery yellow flesh, dominant to pink.
 d - dotted seed coat, black dotted seed when dominant for r, t and w
 dw-1 - dwarf-1, short internodes, due to fewer shorter cells than normal.
 dw-2 - dwarf-2, short internodes, due to fewer cells.
 e - explosive rind, thin, tender rind, bursting when cut.
 f - furrowed fruit surface, recessive to smooth
 g - light green skin, light green fruit, recessive dark green.
 g s - striped green skin, recessive dark green but dominant to light green skin.
 g o - golden, yellow colour of older leaves and mature fruit.
 gms * - glaborous male sterile, foliage lacking trichomes, male sterile.
 l - long seed, long recessive to medium length of seed, interact with s.
 m - mottled skin, greenish white mottling of fruit skin.
 nl - nonlobbed leaves, leaves lack lobbing, dominance incomplete.
 o - oval fruit
 p - pencilled lines on skin, inconspicuous stripes, recessive to netted fruit
 pm - powdery mildew susceptibility, susceptibility to Sphaerotheca fuliginea
 r - red seed coat , interacts with w and t
 s - short seed, epistatic to l.
 Scr - scarlet red flesh
 s u - suppressor of bitterness, non- bitter fruit
 t - tan seed coat, interacts with r and w
 w - white seed coat, interacts with r and t
 W f - white flesh, dominant to red

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 Y0 - orange flesh
 y - coral red flesh
Variations in seed coat colour seed coat colour
Breeding objectives
 Earliness
 Pistillate flowers at lower node number
 Tough skinned fruits for long distance transportation
 Dark red flesh
 Firm and non fibrous flesh texture
 Black seed
 Proper sugar to acid ratio
 TSS content not less than 10% (Arka Manik – 12-15%)
 Firm flesh
 Fruits with smaller and fewer seeds with attractive deep red flesh
 Intermediate fruit shape between typical long and round ones
 High yield (Arka Muthu – 55 to 60 t/ha)

95
 Resistance to disease ,viz., virus, fusarium wilt (race 0,1,2), anthracnose, gummy stem
blight, powdery mildew
 Resistance to insect (cucumber aphids, fruitfly, cucumber beetle, red pumpkin beetle)
GENETIC RESOURCES
 USA – leader in watermelon breeding.
 The USDA watermelon collection is stored at the Regional Plant Introduction Station,
Griffin, Georgia with the back up collection at the National Seed Storage Laboratory,
fort Collins, Colrado.
 1600 accessions in the collection, 300 heirloom cultivars in NSSL
 Germplasm conserved place :IIVR, Varanasi, NBPGR, New Delhi and few SAUs and
IIHR, bangalore.
BREEDING METHODS
 Pedigree method – single seed descent method
 Backcross breding
 Hybrid breeding
SEEDLES WATERMELON
 It requires :
 production of stable tetraploid
 crossing of tetraploid as female with a diploid parent
 Evaluation and commercialization of triploid hybrids.
TETRAPLOID PRODUCTION
96
 Triploid hybrids produce seedless fruit.
 Seedless cultivars are produce by crossing a tetraploid(2n=4x=44) inbred line as the

female parent with a diploid (2n=2x=22) inbred line as the male parent of hybrids.
 The reciprocal cross (diploid female parent) does not produce seeds.
 The resulting hybrid is a triploid (2n=3x=33).
STEPS
 Stage 1: involves choice of diploid lines to use in tetraploid production.
 Stage 2: production of tetraploid plants.
 Stage 3: tetraploid line development
 Stage 4: evaluation of tetraploids as parents of triploid hybrids.
TRIPLOID EVALUATION
 It‘s similar to evaluation of diploid cultivars.
 Commercial production of elite triploid hybrids is done by hand where labor is

inexpensive or by bee.
 The tetraploid and diploid inbreds are planted together in alternating rows, or in
alternating hills within each row.
 The pollen in triploid male flowers is not viable and female flowers in triploid plants
require viable pollen to set fruit.
COMMERCIAL SEED PRODUCTION
 Through manual pollination
In this method tetraploid and diploid lines are planted in alternate rows or in
alternating hills within each row.
Female buds are capped in evening.
Next morning , freshly opened staminate flowers are collected from diploid male
parent and are used to pollinate the pistillate flowers
Pollinated flowers are again covered, tagged . Fruit can be harvested after 40-50
days.
 Pollination through bees in isolation blocks:
tetraploid and diploid are planted in alternate rows in isolation block.

97
During flowering, all staminate flowers from seed parent are removed for a
period lasting several weeks. Pistillate flower on female are tagged with date.
Fruit harvesting is done after 40-50 days after anthesis.
BREEDING MEHODS
 Highly cross pollinated crop. Supported methods are mass selection, pedigree method
and bulk population.
 Modified backcross breeding: Arka Manik – an advanced generation derivative of

modified backcross between IIHR 21 and crimpson sweet. TSS 12-15%, average fruit
weight 6kg. Triple resistant to Powdery mildew, Downey mildew and anthracnose.
 Introduction : Sugar baby, crimpson sweet and Asahi Yamato and New Hampshire
Midget
 Heterosis breeding: heterosis expression for total yield, early yield, and quality
parameters and exploited along with disease resistance eg., Arka Jyoti - Crimpson
Sweet * IIHR 20
 Polyploid breeding: Application of colchicine (0.2-0.4%) morning and evening to the

growing points of young seedlings over a period of 4 to 6 days and soaking of seeds
solution of colchicine(0.4%) for 24 hour.
 Problems
Tetraploid lines are low in fertility.
The cost of seed is high
Yield of seedless watermelon is low
eg: Pusa Bedana- Tetra2xPusa Rasal
 Interspecific hybridization : C.vulgaris readily crossable with Citrullus colocynthis
VARIETIES
 Caltham gray, Summit, Shipper, White hope – resistance to Fusarium oxy f. sp.
 Fairfax – resistance to Alternaria cucumerina
 Alena is a tetraploid from diploid sugar baby
QUALITATIVE TRAITS
 Example include A for monoecious vs. andromonoecious sex expression, Ar-1 and
Ar-2 for resistance to anthracnose races 1 and 2
98
 C for canary yellow flesh color, dw-1 and dw-2 for dwarf vines, E for non explosive
rind, F for non furrowed fruit surface, Fo-1 for Fusarium wilt resistance, gs for striped
green rind pattern, Go for non golden rind at maturity, M for non mottled fruit skin.
 o for oval rather than elongate fruit shape, Pm for resistance to powdery mildew, s
and 1 for short seeds, Scr for scarlet red flesh, yo for orange flesh, and Y for coral red
flesh.
 Crop development: Crimpson Sweet, Sugarlee, Kengarden has dwarf vines.
 ‗Tri – X- 313‘ F1 3x is seedless.
Varieties Breeding Features

methods
PKM-1 It was developed at Horticultural College and Research
Institute, Periakulam. Fruits are bigger in size with dark
green skin and pinkish red flesh. It yields 36-38 t/ ha.
The duration is 120-135 days.
RAU, Rajasthan
Durgapura Selection from Developed at Agricultural Research Station,
Meetha local cultivar Durgapura, Rajasthan.
Durgapura Selection from Yellow fleshed variety
Kesar local type
Durgapura Lal Sugar baby x K-3 Unlobed leaf marker
566
IIHR varieties
Arka F1 hybrid High yielding, good keeping and transport quality

Aishwarya
Arka Akash - -
Arka Manik IIHR – 21 x Resistant to anthracnose & powdery mildew

Crimpson sweet
F1 hybrid
Arka Jyoti IIHR -20 x The fruits are round in shape. Each fruit weighs on an
Crimpson sweet average 6-8 kg, bright crimson flesh with good texture,
flavour and high sugar content (11-13 B TSS) and low
seed content. The rind is pale green in colour with dark
green stripes. The fruits possess good keeping quality
and transport quality. It is an early variety and produces
about 80-85 tonnes/ha. in 90 days.
Arka Madhura Triploid seedless Suitable for year round production under protected
condition.
Amrut It is an F1 hybrid Fruits are oblong in shape weighing 6-8 kg. The skin is
developed by dark green with blackish shade and flesh is dark red. It
MAHYCO. has an yield potential of
100 t/ ha in 95-100 days.
99
VARIETIES AND HYBRIDS
 Pink fleshed – little baby flower
 Golden fleshed – yellow doll
 Red fleshed – crimson sweet, mickylee
 Super sweet – jubilee
 Yellow flesh – golden midget
 Lemon colored dots surface – moon and star
 Miniature – pixie
 Creamy fleshed –cream of saskatchewan
 Scarlet coloured – sangria (non fibrous flesh)
 Pink colour – starbrite
 Amaranth colored fruit – starlight
 Green stripes streaking across its rind – star ‗n‘ stripes
 Dark green rind – sugarbaby (ice box)
 Summer flavour – Crimson sweet + all sweet
 Sweet favorite – light green patches on a blue green rind, ruby coloured fruit
 Yellow baby – similar to yellow doll.
SEEDLESS MELON
 Extazy
 Bijou – round deep red
 Captivation – red flesh, crimson sweet stripe appearance, deep green rind
 Kingman
 SVO 258 WA
 Citation
 Cut above
100
 Harvest moon
 Sweetpolly
 Traveler – deep red(hollow heart tolerance )
 Triple treat – red , ice box type .
VARIETIES
NEW HAMPSHIRE MIDGET

101
ARKA MUTHU
SUGAR BABY
Questions :
102
1.Watermelon varieties released from IIHR
2. What is the origin of watermelon?
4. Name some triploid seedless varieties of Watermelon
5. Name some F1 hybrids of Watermelon
References
L-13
103
MUSKMELON
Scientific name : Cucumis melo
Chromosome number : 2n=2x=24
Origin : Tropical India
Related species : Cucumis anguina, Cucumis sativus, Cucumis ficifolius.
Importance of the crop:
 The musk melons are annuals with climbing, creeping or trailing vines.
 They contain small amounts of protein, fat, minerals, and vitamins.
 Edible portion: Endocarp.
 The desert types quench thirst and add nutrient content of main diet.
 Non desert type melons are used as vegetables.
Melon Botanical varieties:
Scientific name Common name

Cucumis melo var. cantalupensis Cantaloupe, muskmelon
Cucumis melo var. inodorus Winter melon (casaba, honeydew)
Cucumis melo var. flexuous Snake melon, Armenian cucumber
Cucumis melo var. conomon Oriental pickling melon
Cucumi smelo var. chito Mango melon
Cucumis melo var. dudain Pomegranate melon
Cucumis melo var. momordica Snap melon
Cucumis melo var. agrestis Wild type
Cucumis melo var. utilissimus Serpent melon
Cucumis melo var. reticulatus Persian melon
Cucumis melo var. chicko Garden melon
Cucumis melo var. albida New melon
Cucumis melo var. hime Orange melon
Cucumis melo var. saccharinus Pineapple melon
Cucumismelo var. acidulous Sour melon
Cucumismelo var. tamago Egg melon
104
Floral biology and pollination:
Flower:
 The flowers of muskmelon are smaller and less showy

 They are located in leaf axils, most commonly singly.
 Most cultivars are monoecious (producing pistillate and staminate flowers separately),
but a few older cultivars are andromonoecious (producing staminate and pistillate
flowers)
Floral biology:
 The staminate flowers are clustered.

 The pistillate flowers are solitary on short stout pedicels.
 Campanulate calyx with 5 linear lobes, 5 corolla yellow in colour.
 Staminate flowers bearing 3 free stamens.
 Pistillate flowers bear staminodes, an inferior ovary topped by ashort style.
 Stigma – 3 lobed.
 Ellipsoid ovary.
Anthesis : 5:30 to 6:30 AM
Pollination : Highly cross pollinated crop due to monoecious nature.
Stigma receptivity : 2hrs before and 2 hrs after anthesis
Genetics of Muskmelon:
Gene Character
A-G monoecious
Aagg Hermophrodite
Dcl Resistant to Dacuscucurbitae
Jf Juicy flesh
Cytogenetics:
 Chromosome number : 2n=2x=24

 Somatic chromosome number : 2n= 24
 Polyploids with 2n=48
 Cantaloupe is a polyploid with 2n=48
105
 High yielding and earliness.

 Thick skin and flesh with good consistency.
 Good flavour, attractive outer colour and flesh colour texture.
 Small and negligible hollowness of fruit with high TSS not less than 10%.
 Resistance to powdery mildew and downy mildew, CMV, squash virus, cucumber
green mottle.
 Exploitation of heterosis for yield and quality.
 Attractive round shape / spherical fruit shape.
 Attractive orange / green colour.
 Small seed cavity.
 Tough netted skin.
Breeding methods:
MASS SELECTION:
Arka Jeet, Arka Rajhans and MH1 were developed by single plant selection and later
maintained by mass selection
PEDIGREE METHOD:
Pusa Sharbati - Kutana x American Cantaloupe
Punjab Sunehri - Hara Madhu x Edisto
HETEROSİS BREEDING:
It is observed for
1. Days to fruit harvest
2. Early yield
3. Soluble solid content, sugar content
4. Fruit weight
5. Keeping quality, transportability, fruit flavour
6. Number of nodes, vine length
7. Total yield
POLYPLOID BREEDING:
Attempts have been made to produce triploid seedless muskmeons by crossing

tetraploidwith diploids.
106
MUTATION BREEDING:
Male sterile lines have been developed through mutation breeding. The unity of
mutationbreeding is restricted to creation of variability in musk melon.
BACK CROSSING:
The back cross method was combined with selection for resistance to powdery mildew in
field. A superior cultivar Powdery Mildew Resistant Cantaloupe 45 (PMR45) was released.
INTERSPECIFIC HYBRIDIZATION:
The botanical varieties Cucumis melo are intercrossible. Attempt made to cross with
cucumber hasfailed. Somatic hybidization suggested.
Commonly utilized genetic mechanisms for hybrid development:
Mechanism Remarks
Commercial method All monoecious cucurbits
Emasculation and hand pollination Due to andromonoecious flower structure
Use of monoecious lines Problem due to uneven fruit shape
Use of gynoecious lines
Use of GMS system
Breeding Achievements:
RESISTANT VARIETIES
 Downy mildew : Georgia 47, Gulf stream

 Watermelon virus 1 : B665
 Muskmelon mosaic : Oriental pickling melon
 Aphids : p1371795
 Powdery mildew : campo,jacumba
High yieldingvarieties:
 Arkajeet important local selection from lucknow (IIHR 103).

 ArkaRajhans : PLS from local collection from rajasthan (lIHR 107)
 Hara Madfhu, PusaSharbati, PusaMadhuras, DurgapuraMadhu, Punjab Sunheri,
NDMI and NDM2.
107
Varieties / Hybrids Breeding methods Features
IIHR varieties:
ArkaJeet High TSS
ArkaRajhans Tolerant to powdery mildew
IARI varieties:
PusaMadhuras
PusaSharbati Kutana x PMR – 6 (USA)
IIVR varieties:
KashiMadhu Long storage capacity
Punjab Raseela Phoot x Indian cultivar Moderately resistant to

downy mildew
Punjab Sunheri HisarMadhu x Edisto
HisarMadhur
HisarSaras
HisarMadhu Do not slip stage at maturity
DurgapuraMadhu
MHY – 5 DurgapuraMadhu x Hara

Madhu
Jobner 96-2
F1 Hybrids:
PusaRasraj M – 3 x DurgapuraMadhu Utilization of monoecious

line
Punjab Hybrid MS – 1 x Hara Madhu 1 st F1 Hybrid
MH - 10 Gynoecious line x Indian

cultivar
108
Questions:
1.What are all the botanical varieties of muskmelon?
2. Name some varietes released from IIHR
3. What are the breeding objectives of muskmelon?
5. Name some F1 hybrids of muskmelon
References
ICAR, New Delhi.
Agency, New Delhi.
L-14
CABBAGE
Brassica oleracea var. capitata
Cruciferace (2n = 2x = 18)
Origin:
Modern hard-head cabbage cultivars originated from wild non-heading brassicas

somewhere in the easten Mediterrnean and Asia Minor region.
The species Brassica oleracea is divided into:
 Brassica oleracea var. accephala – Kale and collords

 Brassica oleracea var. fimbrieata – Curly kale
 Brassica oleracea var. botrytis – Cauliflower
 Brassica oleracea var. capitata – Cabbage
 Brassica oleracea var. gemmifera – Brussels sprouts
 Brassica oleracea var. gongylodes – Kohlrabi
109
 Brassica oleracea var. italic – Sprouting broccoli
All these species have the c genome the same number of chromosomes (2n = 2x =18)
and readily cross with each other.
CYTOLOGY:
FLORAL BIOLOGY:
A cabbage flower has four sepals, four petals, six stamens in tetradynamous
condition (two short and four long stamens) and a bicarpellary ovary which is superior
and has a false septum. Ovules are attached on both the side of septum. Two active
nectarines are located between the bases of short stamens and ovary. The buds open under
pressure of rapidly growing petals and become fully expanded in about 12 hrs.
Flowers are slightly protogynous and cabbage is naturally cross pollinated due to
sporophytic self-incompatibility. Pollination is brought about by bees and flies.
Bud pollination is effective to achieve selfing. For cross-pollination flower buds

expected to open within 1-2 days are emasculated and are pollinated immediately with
desired pollen using a brush/ flower stamens.
Bud pollination:
The basic seed of parental inbred lines is obtained by hand selfing at bud stage.
The bud top is removed by tweezers and stripers to expose stigma which is then
pollinated with pollen collected from the same plant/line. The seed production plot of
parental inbred lines is covered with net to avoid contamination by bees or other insects.
Pollen grains are collected afresh from the opened flowers on the same day. The mixed
110
pollen grains are collected from the same line should be used for pollination to avoid
viability depression from continuous selfing. If the bagging isolation is applied to
multiply the basic seeds, the flowering branch is covered with paraffin bag/muslin cloth
bag before the bud opens. The bud size should not be too small or big. Bud pollination
done 2-4 days prior flowering gives the highest seed set.
Propagation of basic seeds of incompatible lines by bud pollination is labour
intensive and costly. In consideration of this disadvantage, the electricity-aided
pollination, wire brush pollination, thermal –aided pollination, CO2 enrichment, ect., have
been suggested. However, each one of these has its limitations and has not been used on
commercial scale. Spraying a solution of 5% common salt has been used to overcome the
self-incompatibility and increase the seed set by scientists in china. This method has been
successful in the propagation of basic seeds (Zhiyuan et al., 1999).
Special consideration for F1 hybrids production plots
 Isolation distance of at lest 2000 m from cauliflower, kohlrabi, broccoli, kale,
Brussels sprouts, ect.,
 Provision of approximately 15 honey bee boxes/ha
 Building-up framework through appropriate staking to prevent lodging
 Control of insect and diseases
 Synchronised flowering of the parental inbreds
 Planting ratio of 1:1 for the parental inbreds
 Although harvested seeds from both parents can be mixed-up, it is better to
harvest seeds from both the inbreds separately to improve seed uniformity
111
The shape of the cabbage head can be classified in three groups:
 Ballhead (round head). This is most common type. It has a round 6-7 in. (15-17
cm) in diameter. It has smooth white-veined leaves forming firm head.
 Conical head (oxheart, sugar loaf). This type has a smaller pointed head.
 Drumhead. This type has large flat head.
 High yield
 Longer staying capacity in field after head formation/ greater field holding
capacity
 Desirable heal weight (1-1.5 kg)
 Early head formation and early maturity
 Storage ability
 Head shape and colour as per preference of consumers (essentially round heads,
light green- green colour)
 Less proportion of outer and wrapper leaves
 Short and narrow cone
 Firm head with short internal stem
 Ability to tolerate frost
 Resistance to diseases (Block rot, Alternaria, Black leg)
 Tolerance to insect (Diamond back moth)
112
BREEDING METHODS:
1. Introduction
2. Mass selection
3. Inbreeding
4. Heterosis breeding
Pusa Ageti:
Tropical type, produces seeds under sub tropical conditions, forms marketable
head at a temperature range of 15-30°C but day temperature should not be above 35°C,
recommended for March and August-October planting in northern plains, head weight
600-1200 g, ready for harvest in 75-90 days after transplanting. Yield 110-330 q/ha.
Pusa Drum Head:
Heads are large (3-4 kg), flat, cover leaves are light green, field resistant to black
leg (Phoma lingam) disease, ready for harvest in 80-90 days after transplanting. Yield
300 q/ha.
Golden Acre:
Plants with short stalk and small frame, heads round, very compact cover leaves
with dark green, cup shaped with prominent veins, ready in 60-75 days after
transplanting. Yield 250 q/ha.
Pusa Mukta:
Plants with short stalk heads flattish round, medium in size, leaves are light green
with slightly wavy margins, resistant to black rot (Xanthomonas campestris), ready for
harvest in 70-75 days after transplanting. Yield 300 q/ha.
KGMR-1
Round, compact, green and covered head with outer leaf crimping, medium waxy,
green and slightly serrated leaves, early in heading (60 days), better staying capacity in
the field after maturity (20 days). Average yield(350-400 q /ha).
Questions :
1. Name some species of Brassica oleracea
2. What are all the breeding methods in cabbage?
3. Name cabbage classification based on types of head

113
4. Name breeding achievements in cabbage
5. What is bud pollination techbique in cabbage
References
Agency. New Delhi.
Singh, P.K., S.K. Dasgupta. and S.K. Tripathi. 2004. Hybrid Vegetable Development.
International Book Distributing Co., New Delhi.
114
L-15
Cauliflower
Brassica oleraceae var botrytis
2n=18
Cruciferae
Botany
Leaves :
Leaves are large entire with wavy margin.
Flowers:
Inflorescence is a Raceme , Flowers are complete and bisexual.
Calyx:
4 sepals
Corolla:
4 petals
Androecium:
Tetradynamous.6 stamens(4 long and 2short).
Gynoecium:
Superior ovary of two joined carpels with a single short stled. Ovules are numerous.
Partial placentation but ovary is divided into two chambers b the development of false septum
Fruit:
Siliqua.The siliqua is typically long , slender and smooth with short conical beak.Seeds
are grayish brown in colour.
Leaves with Wavy margin

Seeds
Breeding Objectives:
High yield
Earliness.
White and compactness.
Absence of malformation
Non bolting, erect leaf orient
Resistant to Black rot and tolerant to diverce condition.
BREEDING METHODS
Introduction
115
Mass selection
Pedigree method
Bulk population method
Introduction:
Improved Japanese is an introduction from Israle.
Heterosis breeding:
F1 hybrids: Inbreds are self incompatiable lines are prodused through bud pollination,
late pollination,electrically stimulated pollination,stigma mutilators.
Inbreeding depression in cauliflower is not significantly higher,
SI lines can also be maintained through clonal propagation.
Inter specific hybridization:
Resorted to generate variability in caulliflower.
Intergeneric crosses:
Cross between cauliflower and radish was fairly successful only
whencauliflower acts as male parent. Failure of resiprocal crosses was due to inabilityof the
radish polle to penetrate the styles of the cauliflower.
Breeding Achievement
For different seasons:
Mid September to mid October:
Pusa Katki, Early Kunwari.

Mid October to Mid November:
Pant Shubra, Synthetic 1, Pusa Deepal
December:
Hissar 1, Pusa synthetic , Pusa Shubra.
Late maturity or snowball type:
Snow ball 16,Pusa Snowball1 and Pusa Snowball 2
Resistant to Pest:
Resistant to stiped flea beetle:

Snowball A
Other hybrids:
Self blanched variety:
Pusa Deepali.
Self blanched off season variety:
Pusa Himjyoti , Hisar -1
Tropical Cauliflower
Arka Kanti
Synthetic Variety:
Pant Gobhi-3
Varietal Description:
Arka Kanti:
 Selection from a local collection from Hazipur, Bihar.

 An early tropical cauliflower variety.
 White, compact curds.
Duration 60 days. Yield 22-25 t/ha.
116
Kashi Kunwari:
States for which Released:Punjab, U.P., Bihar and Jharkhand. Characterstics: This is an early
maturity group variety. Suitable time of sowing is end of June to July under Indian plain
conditions. It can tolerate high rainfall during its vegetative growth. Curds are semidome
type, white compact; fine texture and weight about 300450 g; an yield of 300350 q/ha.
Kashi Agahani:
States for which Released: Recommended for Punjab, U.P., and Jharkhand, notified through
Central Variety Release Committee. Characterstics: Midlate maturity group.
Pusa Deepali
Developed at IARI, New Delhi. Recommended for Northern India particularly Delhi and
Punjab. Early maturing variety, curds compact, self-blanching, white, medium sized and
almost free from riceyness.Curds ready for harvest in late October. Average yield is 12 t/ha.
Early Kunwari Recommended for Haryana, Punjab, and Delhi. Very early variety. Curds
hemispherical with even surface, ready for harvesting from mid September to mid October.
Average yield is 8 t/ha.
117
Punjab Giant-26:
Main season variety. Curds solid, white, medium-sized. Ready for harvesting from mid
November to December. Average yield is 17 t/ha.
Punjab Giant-35
Main season variety. Curds white, compact medium sized. Ready for harvesting from mid
November to December. Average yield is 17t/ha.
Pant Shubhra
Recommended for cultivation in Northern India. Early growing variety. Curds are compact,
slightly conical and creamish white. Ready for harvest in November. Average yield is 20 t/ha.
Pusa Snowball-1
Late maturing variety. Curds very compact, medium in size and snow white in colour. Ready
for harvesting from January to April. Average yield is 25-30 t/ha. Susceptible to black rot.
Sonwball-16
Ideal for cooler climates of North Indian states. Late maturing variety. Curds medium sized,
solid, having attractive white colour. Ready for harvesting from January to March. Average
yield is 25-30 t/ha.
Pusa Early Synthetic
Main season variety. Curds somewhat creamy white to white and compact. Ready for harvest
from mid December to mid January. Average yield is 11 t/ha.
118
Pant Gobhi-2
Early maturing variety. Curds compact, composite and creamy white. Curds ready for
harvesting from November to December. Average yield is 12 t/ha.
Pant Gobhi-3
Early maturing variety. Curds medium sized and solid white. Curds ready for harvest from
October. Average yield is 10 t/ha.
Dania Kalimpong
Commonly grown in eastern parts of India. Late season variety. Curds are medium-large,
compact, attractive and white. Less sensitive to fluctuations of environment. Ready for
harvesting from January to April. Average yield is 25-30 t/ha.
Questions
1. What are all the breeding objectives of cauliflwer?
2. Name some early maturing varieties of cauliflower.
3. What are all the breeding methods in cauliflower
4. What is the mode of pollination of cauliflower ?
5. Name some self blanched varieties of cauliflower
References
1. Rana, M.K. 2011. Breeding and Protection of Vegetables. New India Publishing Agency. New
Delhi.
2.Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of Vegetables, Revised, ICAR,
New Delhi.
119
L-16
CARROT
(Hindi – Gajar)
Daucus carota L.
2n = 2 x= 18
Apiaceae
 16 species in the genus Daucus
 - 2 cultivated are D. carota, D. sativus
 Cultivated types are selection from interspecific cross

between the above two species
 Cultivars are classified into 2 types viz., Asiatic and European
 Carrot is an ancient cool season root vegetable.

 Roots are used for making soups, stews, curries, pies, pickles and for salad purposes.
Sweet preparation ‗gajar halwa‘ prepared out of carrot is delicious and popular
 . Roots are also canned. Carrot roots are rich sources of α and β carotenes (1890 μ
g/100g) and contain sucrose 10 times that of glucose or fructose.
 Carrot leaves are a good source of leaf protein. It is sued as fodder and for preparation
of poultry feeds.Carrot has many medicinal properties.
 It increases quantity of urine and helps in elimination of uric acid. It has cooling effect
and is beneficial for people suffering from gall stones, constipation and heat troubles.
 Purple and black carrots are used for preparation of a beverage called ‗kanji which is a
good appetizer.
 In France, essential oil separated from seeds is used for flavouring liquors and all kinds
of food substitutes.
Origin
 Afghanistan is the primary centres of origin of carrot since a large diversity for
morphological and root characters occur.
120
 Considerable variability for root also exists in India, indicating India also as a centre of
origin.
 Root colour varies from absolutely colourless through light lemon light orange, deep
orange, light purple, deep purple to almost black.
Botany
 Carrot is an annual or biennial herb with an erect or branched stem (30-120 cm
high) arising from a thick fleshy root.
 Leaves are pinnate. Edible portion is the fleshy tap root composed of an outer cortex
(phloem) and inner core (xylem).
Floral Biology
 Inflorescence is compound umbel and is produced during second phase.
 Anthesis in a single umbel is completed in 7-9 days.
 Individual flowers are bisexual with white or yellow petals.
 Andromonoecious – Hermaphrodite flowers – periphery & Centre
 - Male flowers – in between the two
Seeds
 They so called carrot seed is actually a fruit, an indehiscent mericarp, which consists of
a single seed.
 Two mericarps pair to form a single rhizocarp, the real carrot fruit which develops
from a two-loculed ovary.
121
Carrot Inflorescence
Carrot seeds
122
Carrot seed
 The so-called carrot seed is actually a fruit, an indehiscent mericarp containing a

single seed.
 In fact, two mericarps pair to form the schizocarp the true carrot fruit.
Root shape and size
 Generally, the carrot root develops from a secondary thickening of the hypocotyl and
tap root.
 In most cultivars, the root first grows in length and then increases faster in diameter
than in length, so that it becomes thicker with age.
 The increase in diameter is most rapid at the crown and comparatively slows down
towards the tip.
 Many physical and biological factors which cause defects such as cooking, forking
and stubbed off roots through injury or interruption of natural tap root growth may
act during this period of early growth.
 Branching of root is quite common and results from certain hereditary factors:
i) presence of undecomposed organic mater or plant refuse in the soil
ii) from injury to young taproot and
iii) from any impediment to its downward growth.
o Heavy soils develop more mis-shapen roots than lighter soils.
o Temperature also affects markedly the shape of roots.
o The typical shape of the cultivar ‗Chantenay‘ was evident only when grown at
18.3 C. At 13 C, the roots were longer and more slender and at 24 C they
were shorter and thicker.
123
o High temperature and irregular water supply usually causes deep horizontal
depressions on the root surface.
o Excessive moisture following drought often results in white corky

outgrowths, where side roots may develop.
o Under high temperature and low rainfall, the shoulders become comparatively
square or sharply rounded .
Root pigmentation
o White, yellow, orange, red, purple and pink types are known.
o The visual colour being determined by level of total carotenoids present, the
accumulation of specific pigments, and the distribution of pigments between
phloem and xylem.
o In some yellow and purple cultivars, the root colour is due to anthochlor and
anthocyanins.
 The red rooted carrot ‘Kintoki’ contains 150-270 mg/ g total carotenoids of
which about 3 per cent are carotenoids.
 The major pigments in this cultivar are lycopene and B. carotene is present in
small amount.
o A temperature range of 15.5 - 21.1 C is found best for colour development.

In fact, carotene content in the roots is weather dependent, especially in the
period preceding harvest, wet weather resulting in low carotene content.
o Similarly carotene synthesis is greater when sunshine is plentiful and rainfall

is scarce.
Cultivars
Cultivars may be classified on the basis of shape of their roots or on temperature

response
A. Classification based on shape:

124
1) Long rooted: Roots may be 25 cm or more in length, generally tapering, performs best in
light soils.
2) Half-long rooted: Root length does not exceed 20 cm.
i) Roots cylindrical with straight or sloppy shoulders (eg.) Nantes.
ii) Roots tapering with blunt or semi blunt tip (eg.) Chantenay, Imperator
3) Short stump rooted: These are suitable for growing in heavy soils.
i) Heart shaped (eg.) Oxheart
ii) Oval (eg.) Early Scarlet Horn
iii) Round (eg.) French Forcing.
B) Classification based on temperature response to flowering
1) Temperate or European or Biennial types
These require low temperature (4.8-10 C) treatment for flowering to occur. These do
not produce seeds in the plains of India (eg.) Nantes, Chantenay, Imperator etc.
2) Tropical, Asiatic or Oriental or Annual types
These do not require any low temperature treatment for flowering to occur. They
seed freely in the plains of India.
Breeding objectives
High yield
High root weight
Good colour
Shape & texture of root
High sugar and carotene
Resistant to pests and diseases.

125
Breeding Method
• Highly cross pollinated
• Heterozygous – Andromonoecy
- Protandry of perfect flower
- Male sterility
- Self incompatibility
Introduction
Imperator, Danvers, Nantes, Perfection
Mass selection
Spontaneous mutation coupled with selection – Cultivated varieties
Mass selection for root length – high yielding lines
Pusa Kesar – Selection from Nantes x Local Asiatic
Mutation breeding
NEU (Nitroso ethyl urea) – 0.025 to 0.6%
NMU (Nitroso methyl urea) - 0.012%
Male sterile mutants
Polyploidy breeding
• Tetraploids (2n=36) and Octaploids (2n=72)
• Only limited utility
Heterosis breeding
126
Earliness, root length, root diameter, core diameter, carotene and yield
Hybrid development – Male sterility
- Self incompatibility
Male sterility
• Functional male sterility (closed anther mutants)
• Structural male sterility (stamenless mutants)
• Genetic male sterility – controlled by plasma gene ‗S‘ and

chromogenes (a and B) with duplicate gene action
The action of ‗a‘ and ‗B‘ genes with duplicate gene action disappears in the presence
of complementary factors ‗C‘ and ‗D‘.
• To generate variability
Varieties
Pusa Kesar
 It is an improved selection from a cross between Local Red and Nantes Half Long.
Rich in carotene content (3.8 mg/ 100 g). This variety can tolerate higher temperature
and is suitable to grow in early September to mid-November.
Nantes
 Roots are orange coloured, slim, well-shaped, cylindrical with stumped end forming a
small thin tail. It has good qualities like thin skin, fine texture and sweet flesh. This
variety is not suitable for seed production in the plains.
Chantaney
 It is a temperate variety highly suitable for canning and storage. Roots are reddish-
orange with a length of 11.5 -15.0 cm and a diameter of 3-5 cm.
127
Zeno
 It is also a temperate variety. It was popularised in Ooty. It is high yielding and

possesses good quality.
Danvers
 It is a mid-season cultivar suitable for fresh market and processing. The roots are deep
orange in colour with ends either tapering or slightly rounded.
Imperator
 It is a cross between Nantes and Chantaney. This mid-season cultivar is suited for
fresh market. The roots are deep orange coloured, 15.0-17.5 cm long and 2.5-4.5 cm
in diameter.
American Beauty
 The roots are orange with self-coloured core, 17.5 cm long, cylindrical with blunt end.
Ooty 1
 It was developed at Horticultural Research Station, Udhagamandalam of TNAU by

half sib progeny evaluation from a local type.
 The plants are robust in growth.
 The roots are 25 cm long with attractive deep orange colour.
 The yield potential is 45-50 t/ ha in a crop duration of 100-110 days.
 This variety is capable of setting seed profusely in the Nilgiris of Tamil Nadu at 2500
m MSL.
Questions
1. Write the classification of carrot .
2. Name the variety released from TNAU
3. What are all the breeding objectives ?

128
4. What are all the breeding methods in crop improvement of carrot ?
5. What are all the breeding achievements in carrot ?
References
1. Dhillon, B.S., R.K. Tyagi., S. Saxena. & G.J. Randhawa. 2005. Plant Genetic Resources:
Horticultural Crops. Narosa Publ. House., New Delhi.
2. Fageria, M.S., P.S. Arya. & A.K. Choudhary. 2000. Vegetable Crops: Breeding and Seed
Production. Vol. I. Kalyani Publ.,New Delhi.
3. Kumar, N. 2006. Breeding of Horticultural crops: Principles and Pracitices. New
India Publishing Agency, Pitam Pura, NewDelhi.
L-18
BEETROOT
Scientific name : Beta vulgaris

Family : Chenopodiaceae
Chromosome no : 2n=2x=18
 Origin :Table beet is originated from the Mediterranean Europe and
North Africa. Later on it spread all over Europe to western India,
forming a secondary diversity centre in the Near East.
Importance of crop
1. Decreases the risk of obesity, diabetes, and heart diseases.
2. Beets contain an antioxidant known as alpha-lipoic acid, which may help lower
glucose levels, increase insulin sensitivity.
3. It is a rich source manganese, thiamine, riboflavin, vitamin, magnesium,

phosphorus, potassium, zinc and copper.
Beta vulgaris classified into three sub species,
 Beta vulgaris subsp. Adanensis

 Beta vulgaris subsp. maritima
 Beta vulgaris subsp. Vulgaris
Other imporatnt species are,
Beta cicla
Beta crispa
129
Beta esculenta
Beta sulcata
Floral morphology
INFLORESCENCE: Large spike
FLOWER: Bisexual, white, Small, Inconspicuous without corolla, but with green
calyx which becomes thicker and covers the seed completely.
MULTI-GERM SEED:
 Also called beet seed

 Fruit containing usually 2 to 6 seeds.
 Small, kidney shaped and brown
 Anthesis -9.00 am -10.00 am Sepals - 4, Polysepalous, erect.
 Petals - 4, Polypetalous, regular, clawed.
 Gynoecium – 2 Carpels, Syncarpous & Superior ovary
 Androecium - 6 Stamens in two whorls.
 2 outer stamens are short,4 Interior stamens are long.
 Anther dehiscence coincides with the Anthesis.
 Pollen viability – On the day of Anthesis , pollen viable for 60 days.
 The stigma is receptive at the time of Anthesis & continues for four days.
Inflorescence
130
Beet leaves
Seed ball
GENETICS
 Root Colour atleast two genes involved.
Intensity of red colour is influenced by minor genes.
 R_Y_ : Red roots , hypocotyls, petioles

 RrY_ : Yellow roots, petioles and hypocotyls
 R_yy : White roots with red hypocotyls
 Rryy : White roots and yellow hypocotyls
Breeding objectives
 High root yield (Detroit dark red)

 Round to globe shaped roots
 Dark red, uniformly coloured roots
 Improved colour and sweetness
 Uniform root shape
131
 Absence of internal white rings in roots

 Increased betalain concentration
 Slow bolting (Avon early)
 Mono-germ seed (khavskaya)
 Resistance to downy mildew, powdery mildew, cercospora leaf spot, rhizoctonia root
rot.
Breeding Methods
 Most of the garden beet cultivars cultivated today are resultant of mass selection
rather than evolved by means of controlled breeding programme.
 The garden beet is a cross-pollinated crop and it is mostly self-incompatible.
 Self sterility in beet can be overcome by sib-mating.
 By using sib-mating for several generations Ohio Canner, a cultivar with a uniformly
dark red flesh colour, has been developed.
 The garden beet cultivars are biennial, and if young plants are exposed to low
temperature, they initiate flowers prematurely.
 Roots of early bolters are usually smaller in size and are more lignified than roots of
normal plant.
 Avon Early, a selection made from a population of 70-80 Detroit cultivars, has been
developed in the U.K. as a bolting resistant cultivar
 Another recent achievement in beet breeding is the development of monogerm

(unilocular seed ball) seeds.
 Khavskaya, a monogerm cultivar, was bred at the Voronesh Experimental Vegetable

Station (U.S.S.R.) using elite seed plants of the cultivar Bordeaux with monogerm and
digerm fruits.
132
 The breeding programme included several selection cycles and inbreeding.

Khavskaya is a mid-season cultivar with spherical or near spherical root, dark red
flesh and a seed germination percentage of 85.
Classification of cultivars
Beet cultivars are usually classified on the basis of shape of roots.
i. Flat - Flat Egyptian
ii. Short top shaped - Flattened at top and bottom with rounded sides.
Eg., Crosby Egyptian, Early Wonder, Asgrow Wonder.
iii. Rounded or globular - Roots are round or globular in shape.
Eg., Detroit Dark Red, Crimson Globe
Half long - Length is shorter than long types
Eg., Half Long Blood, Winter Keeper.
iv. Long - Roots are long, may grow as much as 40 cm, quite popular in Europe
Eg., Long Dark Blood.
The cultivars recommended for cultivation in India are Detroit Dark Red and Crimson
Globe.
Some of the important varieties are,
FLAT EGYPTIAN:
 Introduction from Germany by Ernst Benary Company.

 Early ,small foliage, flat roots.
 Roots are small sized and suitable for canning.
133
FLAT EGYPTIAN
CROSBY EGYPTIAN
 Introduction
 Rounder and smoother than flat egyptian
 It matures in 55-60 days
 It shows pronounced white zoning in warm weather
 Selection
 Roots are perfectly round with smooth, uniform, deep red skin, flesh dark blood red
with light red zoning
 Maturation in 80 – 100 days
CROSBY EGYPTIAN
CRIMPSON GLOBE
 Introduction
 Roots globular to flattened, medium red with small shoulders, flesh medium,
indistinct zoning
AVON EARLY : Bolting resistant variety
134
KHAVSKAYA : Mid season & monogerm cultivar (unilocular seed ball)

BOLDOR- Smooth, golden beet with yellow flesh and an excellent sweet taste
AVALANCHE-white coloured roots
DETROIT DARK RED
Selection
Roots are perfectly round with smooth, uniform, deep red skin, flesh dark blood red with
light red zoning
Maturation in 80 – 100 days
DETROIT DARK RED CRIMPSON GLOBE
AVON EARLY CYLINDRICA

135
AVALANCHE-white coloured roots
OOTY 1
 Selection from the local type.
 It yields on an average of 31.45t/ha of roots.
 The roots are blood red in colour with thin skin and good quality.
 It can be used as a salad. It is a direct sown crop, which can be harvested in 120-130
days, whereas transplanted crop comes to harvest in 135 to 150 days.
 It grows upto 40 to 52 cm in height.
 It yields 1.5 to 2.3t of seed per hectare.
 It can be grown throughout South-Indian hills.
BOLDOR- Smooth, golden beet with yellow flesh and an excellent sweet taste
Questions
1. Write about the beetroot classification
2. Name the variety suitable for south Indian Hills

136
3. What is the origin of beetroot
4. What are all the breeding objectives in beetroot?
5. What is seed ball ?
References
1. Fageria, M.S., P.S. Arya. & A.K. Choudhary. 2000. Vegetable Crops: Breeding and Seed
Production. Vol. I. Kalyani Publ.,New Delhi.
2. Kumar, N. 2006. Breeding of Horticultural crops: Principles and Pracitices. New
India Publishing Agency, Pitam Pura, NewDelhi.
L-19
RADISH
Botanical Name : Raphanus sativus
Family : Brassicaceae
Origin : Europe (or) Asia
Chromosome no : 2n= 18
 Radish (Mooli) is a quick growing and short duration vegetables crop.
 It has high nutritive value.
 It is a good source of ascorbic acid and trace elements. Pink – skinned radish is
generally richer in ascorbic acid than the white skinned.
 Cultivation of radish both for root and seed is very easy.
 Radish is an ancient vegetable. Inscriptions in pyramids in Egypt showed its existence

about 2000 B.C.
 It is widely grown in almost all states of India. It is most suitable for raising in kitchen
garden (or) home garden.
Origin
 Radish probably originated in Europe and Asia. It has been under extensive
cultivations in Egypt since long.
 It was introduced in England and France in the beginning of 16 th century.
Cultivated radish types

137
R. sativus L. var. radicula,
R . Sativus L. var. niger
R. sativus L. var. mougri
R. sativus L. var. oleifera
R. sativus L. var. radicula, R . Sativus L. var. niger
R. sativus L. var. mougri
Botany
 The edible portion of radish develops from the primary root and hypocotyl the
inflorescence is a typical terminal receme of cruciferae.
 The flowers are small, usually white in colour, sepals (four) are erect and petals (four)
are clawed.
 Radish is cross pollinated due to sporophytic system of self- incompatibility.
 It shows considerable in breeding depression on selfing. It is entomophilous. It is

pollinated mainly by wild honey bees and wild- flower flies. Stigma receptivity is
maintained upto four days after anthesis.
Leaf
138
Attitude : erect, semi- erect (or) horizontal
Length : short, medium (or) long.
Leaf blade
Shape : Narrow- obovate, obovate (or) broad obovate, pointed (or) rounded
Colour : yellowish- green (or) greyish green
Pubescence : Slight, medium (or) strong
Petiole
Anthocyanin coloration : absent (or) present
Intensity of anthocyanin coloration : weak, medium (or) strong.

139
Cytogenetics
Basic and haploid chromosome numbers of radish X=n=9.
Chromosome size range 1.5- 3.5 m
Basic genome of radish has both homologus and non – homologous chromosome.
Basic cytological and phylogenetics relationship, the species of raphanus evolved from a
commom ancestral form with chromosome number 2n= 6 by chromosome duplication.
Breeding objectives
 Early rooting
 High yield ( Arka Nishant- 18- 20 t/ha)
 White, long/stump roots with thin tap root and non- branching habit
 Non pithy roots
 Pungency of roots as per consumers preference
 Slow bolting habit
 Heat tolerance
 Drought resistance
 Wet tolerance
140
 Resistance to alternaria blight, white rust, RMV
 Tolerance to aphids
Breeding Methods
Mass selection
This is practiced in cultivars collected from the farmer‘s fields.
Arka Nishant from IIHR.
Pedigree method
Pusa Himani : Black Radish × Japanese White
Pusa Rashami : Green Top × Desi
 Polyploid breeding
 Polyploids with 2n= 36 produced. No distinct advantages. Two polyploid varieties

have been developed and these yield more than diploids.
 Sofia Delicious (2n=36)
 Semi long Giant (2n= 36)
Variety and varietal character
A. European (or) Temperate Types
1. White lcicle
 The roots are thin and tender and icicle shaped. The skin of roots of this variety is
pure white.
 It is a flavoured variety. It is very short duration (20-25 days) and suitable for
kitchen garden.
Delay in harvesting causes pithiness in roots that reduces quality of roots. It is sown in
oct-nov in plains.
141
White lcicle
2. Scarlet Globe
It is an early variety takes about 25-30 days for development of harvestable

roots, outer skin of roots is scarlet in colour with white tip, and flesh is pure white.
Roots are round in shape.
Scarlet Globe
3. Scarlet Long
It is an early variety (25-30 days). Leaves are 15-20 cm in length and light
green in colour. The roots are long and tapering to a point.
B.Tropical (or) Asiatic types
1. Japanese White
It in introduction from Japan,c.v. ―shiroaguri- kiyO‖ recommended by IARI

Regional Station, Katrain (HP).
The roots are pure white, 20-30 cm (or) so long, blunt at root –end.
142
They are mildly pungent.
It is suitable for Oct- Dec sowing.
Japanese White
2.Pusa Desi
Roots are long (30-35cm) white, tapering with green stem-end variety is
suitable for sowing in early Aug- Oct in two northern plains of India, roots become
ready for harvesting in 45- 50 days
Pusa Desi
3.Pusa Chetki
It is developed by IARI, New Delhi from the seeds collected from Denmark.
Roots are pure white and they are mildly pungent.
The leaves are entire, upright, dark green and slightly lobed.
143
Pusa Chetki
4. Pusa Reshmi
It was developed through recombination breeding, released by IARI, New

Delhi.
The roots are white, long(30-35cm), tapering with green stem end.
The foliage is medium , light green with cut leaves.
Pusa Reshmi
5. Punjab Safed (White 5 × Japanese White)
The roots of this variety are white, tapering, mildly pungent and 30-40cm
long.
The roots are ready for harvesting in 40-45 days.

144
Punjab Safed
6. Kalyani White
The roots are pure white having blunt ends,25-30 cm long. Top growth is
short.
The roots are mildly pungent. This variety is suitable to grow all the year
round except during hot summer.
Roots become ready for harvesting in 45-50 days.
Kalyani White
7. Chinese pink
An introduction identified by Dr. Y.S.Parmar. University of Horticulture

and Forestry, Nauni, Solan (HP).
The roots are 10 -15 cm long pink with white colour towards the tip. The
roots are ready for harvesting in about 50- 55 days.
145
Chinese pink
8. Arka Nishant
This variety was developed from the seed collected from Singapore; and
released byIndian Institute of Horticultural Research, Banglore in 1980.
The roots are mildly pungent, marble white in colour, non –pithy roots of
excellent quality.
Pre mature bolting and root forking are not commonly seen.
Resistance Breeding For Biotic Stress
Asmer Tip Top: To insect Erioischia brassicae
Punjab Safed : To Leaf spot
Nerina : To Mosaic Virus
Summer Wonder: Res. To Black rot
Chinese Rose White: Res. To Downy Mildew
Questions
1. What are all the cultivated radish types?
2. Name some resistant varieties of radish to some biotic stresses.
3. Name some varieties developed from IARI?
4. Name a variety developed from IIHR
5. Name a radish variety with pink skin.
References

Rana, M.K. 2011. Breeding and Protection of Vegetables. New India Publishing Agency.
New Delhi.
146
L-20-21
BIG ONION (or) COMMON ONION
 Scientific Name : Allium cepa

 Chromosome no :2n = 16
 Origin – Pakistan(According to Vavilov)
 Inflorescence: in each flower – 6 stamens, 3 carpels, 6 perianth segment
IMPORTANCE OF THE CROP
 Onion is cool season vegetable
 It is grown for its bulbs which are used as salad, cooked in various ways in all curries,
fried, boiled, used in soup making in pickles and many other purpose
 In India, the most important onion growing states are MH, AP, TN, Bihar and punjab.
 It is a export oriented crop
ORIGIN AND DISTRIBUTION
 The Russian scientist Vavilov reported central Asia is the primary center of origin.
 Western Asia and area around Mediterranean Sea are its secondary centers of origin.
 Now it is distributed throughout the world.
FLORAL BIOLOGY
 The flowering structure of onion is called umbel.
 Umbel is an aggregate of many small inflorescences of 5 to 10 flowers.
 The inflorescence may consists of a few to more than 2000 flowers for umbel
 Anthesis occurs in early morning (6-7 hrs). Anther dehiscence is between 7.00 and
17.00 hr and on next day also with peak between 9.30 and 17.00 hr.
 Pollen fertility is highest on the days of anthesis. Stigma receptivity is also high on the
day of anthesis.
147
148
Onion flowers
Floral Formula: Br ⊕ P3+3 A3+3 G(3)

149
Onion seed head Onion seeds
MODE OF POLLINATION
 Onion is highly cross pollinated crop and pollinators are bees and other insects
 The reason for cross pollination is heteromorphism and male sterility.
Related species
150
Tree onion-bulblets are produced in flowerheads-hybrid between Allium cepa and

Allium fistulosum
Allium canadense (Wild onion)
CHIVE- Allium schoenoprasum

151
Allium porrum (LEEK)
SHALLOT- Allium ascolonicum

(2-3 individual bulbs inside)
WELSH ONION
OBJECTIVES OF BREEDING
 Varieties with improved quality(good shape, colour) and high yield suited to different
agro climatic conditions and better stability
 Varieties resistant to diseases like basal rot and phythium blight etc.
 Develop to short day, high yielding F1 hybrids With longer storage life.
 Development of white onion varieties suitable for dehydration and export.

152
 High yield
 Longer bulb storage life
 Resistance to diseases ( purple blotch, basal rot, stemphyllium blight )
 Resistance to insect pests(thrips)
 Resistance to abiotic stress
 Single centered bulbs especially for fresh market
BREEDING METHODS
MASS SELECTION:
 Arka Kalyan developed from MS from IIHR-45 (moderately resistant to

purple blotch).
 Arka Niketan: developed from MS from IIHR-153(red colour excellent
keeping quality)
PEDIGREE METHOD: Arka Pitambar: Yellow onion.
HETEROSIS: Arka kirthiman:(tolerant to purple blotch)
INTERSPECIFIC HYBRIDIZATION:
 A. cepa var. Cepa X A. fastulosum –welsh onion popular in china and spain
Salient breeding achievements
 IARI: Pusa Red, Pusa Madhushri, Pusa Ratnar, Pusa White Round.
 NHRDF: Agri found Dark Red, Agri found Light Red.
 TNAU: CO1 to CO4-All red
 PAU: S48,Punjab selection-Red
 MPKV:NS-4-1,Baswant 780-Red
Qualitative Genetics
 Inheritance of bulb colour in onion:
 Colour classes: White, Yellow, Red and Brown
 Dominant basic colour factor ‗C‘ is necessary for either red or yellow
colour(Clarke et al 1944).
153
 All the plants with ‗cc‘ have white bulbs regardless of presence of other colour
factors.
 Dominant ‗R‘ with ‗C‘: red bulb colour
 ‗r‘ with ‗C‘: Yellow bulb
 ‗I‘ colour inhibiting factor incompletely dominant over ‗i‘.
 ‗II‘ : White bulb colour regardless of presence or absence of C and R factors.-
Sources of resistance to diseases and insect pests in onion
Diseases/Insect pests
Level of
(causal Name of the variety/line
resistance
organization)
A. Diseases
i) Purple blotch Resistant ‗IHR 56-1‘
(Alternaria porri Moderately ‗Red Creole‘, ‗33-2‘, New Selection
(Ellis) Cliff.) resistant and VL-67
Pusa Red,
VL-1, N-2-4-1, Ph. Sel-3,
Pusa Ratnar and Pb. Sel-5
IHR 25, IHR 471, IHR 500 and Arka
Kalyan
Tolerant Rampur Local, Pusa Red and Patna
Red
ii) Stemphylium Tolerant 40 accessions
blight
(Stemphylium
vesicarium
(Wallroth))
iii) Stemphylium Combined IC 32176, 42900, 47954, 47972-3,
blight + purple tolerance 47997, 48001, 421452-2, 48710,
blotch 48724, 48954, 48903, 49012 and
49121, 48268-1, 48548, 48575
iv) Basal rot Resistant Telagi Red, White large, Poona Red,
(Fusarium Bellary Red, Udaipur 103, Patna Red
oxysporum f.sp. and N-257-9-1
Cepae, Schlect.) IHR 141, IHR 506 and Sel-13-1-1
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B. Insect pest
i) Thrips (Thrips Resistant N-2-4-1, Sel-104, Pusa Ratnar, Sel-
tabaci Lind.) 171, HR Brown & Sel-1202.
No. 544, Kalyanpur Red Round,
Udaipur 103, Safed Gol, No.5,
White, Mathewad-1, Shirwal-2,
White Creole, Kagar-2 & Peth -1
Tolerant Hissar-2, Panipat Local, Rajpur Red,
Onion Karachi, Pusa Ratnar, Bombay
White, N-53, Red & white big round
Lowest Allium fistulosum, BS 956, BS 929,
incidence BS 968 and K-1-7-1,
Ludhiana Selection, N-2-4-1
Hissar-2, 123-7-1, Bombay white
ii) Gram caterpillar Least Sel-102-1, Large red S-76, Verma
(Heliothis preference Giant, S-207, S-243 & S-16 (seed
armigera) crop)
Some important varieties in India and their characters
1.Pusa Red IARI ,New Delhi Red colored bulbs, good storage
2.Pusa Ratnar IARI New Delhi Deep red, round, yield 30 to 40t/ha
3.Arka IIHR, Banglore Good for storage
Nikethan
4.Arka Kalyan IIHR, Moderately resistant to purple blotch
Banglore
5.Arka Bindu IIHR, Banglore Suitable for export
6.Arka Lalima IIHR, Banglore Resistant to purple blotch

7. Pusa white IARI ,New Delhi Suitablefor dehydration
flat
8 .Punjab 48 PAU, Ludhiana Suitable for dehydration
9.Early Grano IARI, New Delhi Yellow onion Good for salad purpose
10.Agri found NHRDF Suitable for kharif
Dark red
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Agri found Dark red
Agri Found Light Red
Pusa Riddhi:
 Single bulb weight ranges from 70.0 -100.0 g.

 Pungent and rich in antioxidant (quercetin107.42 mg/100g).
 Suitable for kharif and crop,, and export.
 Average. Yield: 31.66 t/ha.
156
Pusa Soumya
 First bunching onion variety
 suitable for round the year green onion production.
 least affected by pests and diseases.
 Yield: 26.38 t/ha.
Arka Akshay:
It is a tri-parental synthetic variety with dark Red globe shaped bulbs. Average bulb
weight is 115 g. Yield 45 t/ha in 130 days.
Arka Bheem (Syn-6) :

157
 It is a tri-parental synthetic variety

 Red to pinkish red, elongated globe shaped bulbs
 Average bulb weight is 120 g
 Yield 47t/ha in 130 days.
Arka Sona
Arka Sona has the yellow bulb colour, globe shape, big size of bulb diameter 6.5-7cm,
bulb weight 140.0g, low TSS 100 B, bulb yield 45 t/ha, duration 120 days, suitable
for growing in Rabi season and suitable for export.
Arka Vishwas
Arka Vishwas has the bulb colour dark red, flat globe shape, small size of bulb diameter3-
4cm, bulb weight 40.0g, TSS 160 B, bulb yield 30t/ha, duration 115 days, suitable
for growing in Rabi season and suitable for export.
158
Arka Swadista :
 white onion variety

 bulbs are oval globe in shape,
 TSS 18-20%,
 edible bulb 98%, weight 35-40g,
 suitable for bottle preservation.
 Yield =16-18t/ha in 105 days.
Arka Swadista :
Arka Kirthiman :
A F1 between MS-65 x Sel. 13-1-1 Medium size bulbs with globe shape and firm
texture. Bulbs red in colour with bulb weight 120 -130 g. Tolerance to purple blotch,
basal rot & thrips. Long storage (4-5 months) Suitable for kharif and rabi seasons.
Duration 125-130 days. Yield 47 t/ha.
Arka Kirthiman
Arka Lalima
 F1 hybrid. Suitable for Kharif and Rabi Bulb yield: 50 t/ha

159
Arka Kalyan:
Developed through vigorous mass selection from IIHR-145 Globe shaped bulbs with medium
large size. Deep red coloured outer scales and fleshy succulent internal scales. Average bulb
weight 130-180g. Pungent with TSS 11-13%. Moderately resistant to purple blotch caused by
Altermaria porii Seed yield 8 quintals/ha. Suitable for kharif season. Duration 140 days.
Yield 47 t/ha.
Arka Pitambhar
Developed through pedigree selection from the cross U.D. 102 x IIHR-396. Bulbs
uniform yellow. Medium size (5.2-6.0cm) bulbs with globe shape and thin neck. Mild
pungent with TSS 11% and total sugar 9.81%. Tolerance to purple blotch, basal rot diseases
and thrips. Long storage life (3 months). Suitable for export market Suitable for kharif and
Rabi seasons. Duration 140 days. Yield 35 t/ha.
160
VARIETIES / F1 HYBRIDS FROM NHRDF, Nasik

 Agrifound Dark Red
 Agrifound Light Red
 NHRDF Red
NHRDF Red
VARIETIES / F1 HYBRIDS FROM

IARI, New Delhi
 Pusa Red
 Pusa Ratnar
 Pusa Madhvi
 Pusa White Round
 Pusa White Flat
VARIETIES / F1 HYBRIDS FROM VPKAS, Almora

 VL 1
 VL 3
VARIETIES / F1 HYBRIDS FROM MPKV, Rahuri
— Nasik 53
— N 2-4-1
 Baswant 780
 N 257-9-1
 — Phule Suvarna
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Nasik 53
VARIETIES / F1 HYBRIDS FROM TNAU, Coimbatore
 CO 1
 CO 2
 CO 3
 CO 4
VARIETIES / F1 HYBRIDS FROM RAU,Bikaner
 Udaipur 101
 Udaipur 102
Udaipur 103
Varieties /F1 hybrids from DOGR, Rajagurunagar
Bhima Raj
 Suitable for kharif and late kharif season the states of Maharashtra, Karnataka
and Gujarat.
162
Bhima Red
This variety already recommended for rabi season in Maharashtra and Madhya
Pradesh, is also recommended for release for kharif season in Delhi, Gujarat, Haryana,
Karnataka, Maharashtra, Punjab, Rajasthan and Tamil Nadu. It can also be grown in
late kharif. Maturity is 105-110 DAT during kharif and 110-120 DAT during
late kharif and rabi seasons. The average marketable yield in kharif season is 19-21 t/ha, in
late kharif season is 48-52 t/ha and it is 30-32 t/ha in rabi season.
It can be stored up to 3 months. in rabi.
Bhima Super
A red onion variety from DOGR has been identified for release for kharif season in
Chhattisgarh, Delhi, Gujarat, Haryana, Karnataka, Madhya Pradesh, Maharashtra, Odisha,
Punjab, Rajasthan and Tamil Nadu. It can also be grown in late kharif. It is reported to have
an average yield of 20 - 22 t/ha in kharif and 40 - 45 t/ha in late kharif. Bulbs attain maturity
within 100-105 days after transplanting (DAT) in kharif and 110 -120 DAT in late kharif. It
produces mostly single centered bulbs.
163
Bhima Kiran
 good keeping quality suitable Storage for upto 5 to 6 months
Bhima Shakti
 Very less number of bolters 2.15%
Bhima Shweta :
164
This white onion variety already recommended for rabi, is also recommended for
release for kharif in Chhattisgarh, Gujarat, Karnataka, Madhya Pradesh, Maharashtra, Odisha,
Rajasthan and Tamil Nadu. TSS is around 11-120B and it matures within 110-120 DAT. It
has medium keeping quality and can be stored up to 3 months. Average marketable yield
during kharif season is 18 - 20 t/ha and in rabi is 26-30 t/ha. It is tolerant to thrips
Bhima Shubra
This white onion variety has been recommended for Chhattisgarh, Gujarat, Karnataka,
Madhya Pradesh, Maharashtra, Odisha, Rajasthan and Tamil Nadu for kharif season. It is also
recommended for late kharif in Maharashtra. It matures in 110-115 DAT during kharif and
120-130 DAT in late kharif. TSS is 10-120B. It is a medium storer with a capacity to tolerate
environmental fluctuation. Average marketable yield during kharif is 18 - 20 t/ha and during
late kharif 36-42 t/ha. It has capacity to tolerate environmental fluctuation, hence can be
cultivated in all season
The acceptable Indian onion varieties for dehydration among white flesh onions are
 Bombay White
 No-36-1-3-4 ‗
 Udaipur-102‘,
165
 ‗S-74‘,
 ‗Pb-48‘,
 ‗L-131‘,
 ‗L-124‘,
 ‗L-106‘,
 ‗Pusa White Round‘,
 ‗Pusa White Flat‘, ‗
 N-257-9-1‘ etc.
 Generally, Indian white onion varieties have low TSS (10-14%),

which is not suitable for dehydration.
 After assessing Indian varieties and land races which do not have high TSS, Jain Food
Park Industries, Jalgaon, introduced White Creole, which was further subjected to
selection pressure for high TSS and they developed V-12 variety with TSS range of
15-18%.
 TSS in any variety is a function of genotype, environment and cultural practices.

Long day onion grown under mild climate is high in TSS, whereas, short-day onion
maturing under short winters does not develop high TSS. Internationally, long-day
and intermediate short-day varieties have been developed mostly from USA, Spain,
Israel, Mexico, etc.
Molecular markers for colour improvement in onion
 Bulb colour is one of the important traits in onion (A. cepa L). Three major colours of
white, yellow, red and a variety of other bulb colours such as chartreuse and gold
exist in onion germplasm.
 The bulb colour is due to flavonoid compounds and 54 kinds of flavonoids have been
reported in onion.
 Critical mutations in the chalconeisomerase (CHI) gene causing gold onions were
identified.
 The inactivation of dihydroflavonol 4-reductase (DFR) in the anthocyanin

synthesis pathway was responsible for colour differences between yellow and red
onions, and two recessive alleles of the anthocyanidin synthase (ANS) gene were
responsible for a pink bulb colour .
 Based on mutations in recessive alleles of these two genes PCR based markers were
developed for identification of polymorphisms between pink and red alleles of the
166
ANS gene. Most pink onions were homozygous recessive for the ANS gene
indicating the homozygous recessive. The two pink onions, heterozygous for the
ANS gene, were also heterozygous for the dihydroflavonol 4-reductase (DFR) gene
indicating that the pink colour was produced by incomplete dominance of a red
colour gene over that of yellow colour.
 Functional CAPS markers were developed for two inactive DFR-A alleles, DFR-APS
and DFR-ADEL, for detection of inactive DFR-A alleles responsible for a failure of
anthocyanin production in onions. Of these two alleles, DFR-APS predominantly
occurs in yellow onion cultivars.
Breeding for yellow onion
 Indians do not prefer yellow onion but these find international market in European.
Minimum requirements for export are: bigger sized (>60 mm diameter), less pungent
and single-centered types.
 ―NuMexStarlite‖,a new yellow-onion variety

developed which was
resistant to bolting and the short-day type was
obtained by 5 recurrent selections from TexasGrano502PRR.
 Texas ‗Grano 1015 Y‘, a mildly pungent, sweet, short-day yellow onion variety, was
developed through original, single-bulb selection from Texas Early Grano 951
through 5 generations of selections.
 Similarly, ―Texas Grano1030 Y‖ was developed from F2 selections of Texas Early

Grano 502 x Ben Shemen which is a late maturing mildly pungent short- day onion
variety.
 Only two varieties were developed, viz., Phule Swarna from MPKV, Rahuri and Arka
Pitambar from IIHR, Bangalore and were released at the state / institute level. Yield
of these varieties was comparatively less than in commercial red onion varieties. 12
varieties of onion were assessed during kharif season and found lowest bulb diameter
of 4.2 cm in Arka Pitambar, along with low yields.
Questions
1. Breeding objectives in onion

2. Write about Floral biology of onion
3. Name some varieties developed from IIHR
4. Name some varieties developed from IARI
5. What are all the methods of breeding in onion.
References
167
1. Gupta, S.K. 2008. Plant Breeding : Theory and Techniques , Motilal UK Books of
India (2008).
MULTIPIER (or) AGGREGATUM ONION
Allium cepa var. Aggregatum
Unlike the bellary onion, the propagation of aggregatum onion is through bulblets
(Vegetative propagation).
Breeding objectives
 bulbs with bright red colour
 high yield
 bold bulblets
Varieties
CO 1
It is a clonal selection from CS 450, an onion type introduced from Manachanallur,

suitable for cultivation in all the onion growing districts of Tamil Nadu. It has an yield
potential of 9-10 t/ ha in a crop duration of 85 days. The bulbs are large and pink in colour.
CO 2
It is a selection from germplasm type CS 911. It has an yield potential of 12 t/ ha in a

crop duration of 65-70 days. Bulbs are bigger in size with crimson colour.
CO 3
It is a clonal selection from the open pollinated progenies of CS 450. The crop
duration is 65-70 days. It yields 15-16 t/ ha.
CO 4
168
It is a hybrid derivative of the cross AC 863 x CO 3. The crop duration is 65 days.

It yields 10 t/ ha. Each clump weighs on an average of 90 g. Bulbs are bold in size with
attractive pink colour. The bulbs store well for more than 150 days devoid of sprouting in
well ventilated store rooms.
Agri Found Red
Developed by NHRDF Dindugal. Each clump weighs 65 g with 5-6 bulblets per
clump. The colour is light red. TSS 15-16 brix. It can give 18-20 t/ ha in 65 days.
Arka Ujjwal:
 A multiplier onion variety
 bright dark red bulb color, compound bulb with flat shape
 Bulb size 4-5cm,
 Number of bulblets/bulb 3-5,
 Bulb weight 40-45g,
 TSS 16-18%,
 Dry matter content 14-16% and
 Bulb yield 20-25t/ha in 85 days.
Arka Ujjwal:
COOn 5 (2001)
It is a high yielding variety developed by mass pedigree method of selection. This
variety has the ability of free flowering and seed set throughout Tamil Nadu. It possess high
169
bulb yield 18.9 t/ha (18.8 per cent higher than CO.4) in a crop duration of 90 days. It is free
flowering type with seed setting ability of 250-300 kg/ha and so it is propagated through
seeds. March–July for bulb production and November– January for seed production. It
possesses attractive pink coloured bold size bulbs. The total soluble solids content is 13 per
cent. The pungency principal measured as pyruvic acid is 2.37 µm/g of fresh weight. It is
adaptable to Coimbatore, Trichy, Pudukottai, Nagapattinam, Thanjavur, Tiruvarur, Theni,
Madurai, Namakkal, Cuddalore, Tiruvannamalai, Thoothukudi, Erode and Dharmapuri
districts.
Questions
1. Breeding objectives in onion

2. Write about Floral biology of onion
3. Name some varieties developed from TNAU
4.Name some varieties developed from IIHR
5.What are all the methods of breeding in onion.
References
Gupta, S.K. 2008. Plant Breeding : Theory and Techniques , Motilal UK Books of
India (2008).
GARLIC L-22
Allium sativum L. Family: Alliaceae (2n=16)
Garlic (Allium sativum L.) is the second most widely used cultivated allium after
onion. It is a very hardy herbaceous perennial species, which has been recognized as a
valuable spice for foods and a popular remedy for several ailments and physiological
disorders.
It is a herbaceous annual for bulb production and a biennial for seed production. The
edible underground stem is a composite bulb made of numerous smaller bulbs known as
cloves. If very small cloves are planted or if the growing conditions are poor, single solid
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clove usually called ―round‖ is produced. ―Rounds‖ if planted under favourable conditions
give rise to usual composite bulb.
Many clones of garlic do not produce flower stalks or the inflorescence may be
partially or not at all exerted, its bulbils forming a swelling somewhere within the false stem
one to five centimeter above the bulb.
The typical flavour of garlic is due to the presence of the chemical allein plus diallyl
disulphide. It is a cross pollinated crop. The yield potential depends on the amount of
vegetative growth made before bulbing commences and as soon as the bulbing commences
leaf initiation stops. Generally after 6 to 8 leaves, bulbing commences. At the base of each
leaf sheath, 3-5 cloves will be formed in 4 to 5 leaves depending on the varieties. A total of
12 leaves are produced under normal conditions.
Nutritive composition of fresh by peeled garlic cloves and dehydrated garlic powder is as
follows:
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Nutritive Value of Garlic
Fresh peeled garlic Dehydrated garlic

Particular cloves powder
Moisture (%) 62.80 5.20

Protein (%) 6.30 17.50
Fat (%) 0.10 0.60
Mineral matter (%) 1.00 3.20
Fibre (%) 0.80 1.90
Carbohydrates (%) 29.00 71.40
Energy K. Cal 145.00 --
Calcium (%) 0.03 0.10
Phosphorus (%) 0.30 0.42
Potassium (%) -- 0.70
Magnesium (mg/100 g) 71.00 --
Iron (%) 0.001 0.004
Niacin (%) -- 0.70
Sodium (%) -- 0.01
Copper (mg/100 g) 0.63 --
Manganese (mg/100 g) 0.86 --
Zinc (mg/100 g) 1.93 --
Chromium (mg/100 g) 0.02 --
Vitamin A (IU) 0.40 175.00
Nicotinic acid (mg/100 0.40 --
g)
Vitamin C (mg/100 g) 13.00 12.00
Vitamin B (mg/100 g) 16.00 0.68
Riboflavin B2 (mg/100 0.23 0.08
g)
Thiamin (mg/100 g) 0.06 --
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Healthy garlic bulbs contain allicin, colourless,

odourless and water-soluble amino acids. On crushing the
garlic bulbs the enzyme allinase breaks down into allin to
produce allicin of which the principal ingredient is
odoriferous diallyl disulpide. Garlic contains about 0.1%
volatile oil. The chief constituents of oil are diallyl disulfide
(60% ), diallyl trisulfide ( 20% ), allyl propyl disulfide ( 6%
), a small quantity of diethyl disulfide and probably diallyle
polysulfide. Diallyle disulfide possesses the true garlic
odour. Garlic has been cultivated for thousands of years. It is
the most ancient cultivated vegetables giving pungency of
the genus Allium. Original abode of garlic is said to be
Central Asia and Southern Europe especially Mediterranean region. Some authorities
consider that Allium longicuspis Regael, which is endemic to Central Asia, is the wild
ancestor and spread in ancient times to Mediterrenean region. It is known in Egypt in
Predynastic times, before 3000BC and also to ancient Greeks and Romans. It has long been
grown in India and China. Garlic was carried to the Western hemisphere by the Spanish,
Pourtuguese and French. Garlic was not liked by Romans due to strong odour. It was used in
England as early as first half of the 16th century. The early domestication of garlic took quite
different turn from that of seed propagated leek and onion. Garlic became exclusively
vegetatively propagated by cloves or bulbils. Some cultivars are reported to produce flowers
but there is no seed setting. Garlic cultivars differ in maturity, bulb size, clove, clove size and
number, scale colour, bolting and floweringhabits
VARIETIES
Garlic varieties, Agrifound White, Yamuna Safed, Yamuna Safed 2 and Yamuna Safed 3
have been notified by the Government of India. The varieties developed by NHRDF are
given below:
AgrifoundWhite :
The variety was notified by Govt. of India in 1989 vide notification no. 28(E) dated
13/4/1989. The variety was developed by mass selection from a local collection obtained
from Biharsharif area in Bihar. The bulbs are compact, silvery white with creamy flesh.
Bigger elongated cloves with 20-25 in numbers. Diameter 3.5 to 4.5 cm size index 12-15
173
cm2. The variety is susceptible to purple blotch and stemphylium blight which are common
in the northern parts. TSS 41% dry matter 42.78% and good storer. The yield is 130 q/ha. It is
recommended for cultivation in the areas where there is not much problem of purple blotch or
stemphylium blight in rabi season.
YAMUNA SAFED (G-1)
This variety was notified by Govt. of India in the

year 1991 vide notification no.527 (E) dated
16/8/1989. It was developed by mass selection from a
local collection obtained from Delhi (Azadpur)
market. The bulbs are compact, silvery white skin
with creamy flesh. Diameter 4.0 cm to 4.5 cm. Sickle
shaped cloves, 25-30 in number, size index of bulb
12-15 cm2, diameter of cloves 0.8 to 1.00 cm. The
variety is tolerant to insect pests and diseases like purple blotch, stemphylium blight and
onion thrips. TSS 38%, dry matter 39.5% and good storer. Yield 150-175 q/ha. It is
recommended for cultivation all over the country.
YAMUNA SAFED-2 (G-50)
The variety was notified by Govt. of India in the year 1996 vide notification no.115 (E) dated
10/2/1996. It was developed by mass selection from a local collection obtained from Karnal
area in Haryana. The bulbs are compact attractive white creamy flesh, bulb diameter 3.5-4.0
cm, size index 11-12 cm2,, number of cloves 35-40, diameter 0.75 - 14 cm. Clove size index
1.75-2.5 cm2,, 10 bulb weight 160-240 g, TSS 38-40%, dry matter 40-41%. Average yield
150-200 q/ha. The variety is recommended for Northern India.
The variety has done very well in Northern parts and also in Central parts of India. It was
developed by mass selection technique from a local collection obtained from Dindigul (TN)
in 1990. The leaves are wider than other varieties. Bulbs are creamy white and bigger sized
(5-6cm dia). Size index 27-29cm2, diameter of cloves 1.2-1.5 cm. 15-16 number of cloves
per bulb TSS 38.42%, dry matter 39-43%, medium storer. Average yield 175-200 q/ha. The
174
variety is suitable for export. The variety was notified in the year 1999 vide notification
no.1092 (E) dated 26/10/1999.
AGRIFOUND PARVATI
This variety was developed in 1992 by selection from an exotic collection obtained
from Hongkong market. The variety is long day type and as such is suitable for cultivation in
mid and high hill of Northern states. Bulbs are of bigger size (5-6.5 cm), creamy white colour
with pinkish tinge. Size index 16-72 cm2, diameter of cloves 1.5 to 1.8 cm, 10-16 cloves in
number tolerant to common disease. Average yield 175-225 q/ha, medium storer. Suitable for
export.
The variety has done very well in Zone VI. It was developed by mass selection
technique from a local collection obtained from Jaunpur, Uttar Pradesh in 1988. Leaf wide,
Bulb- compact, attractive creamy white colour, creamy flesh, bulb diameter 4-5 cm, size
index 14-16 cm2, no. of cloves 18-23 per bulb, diameter of cloves 0.75-1.0 cm, cloves size
index 1.8-2.0 cm2, Matures in 165-175 days. Yield 200-250 q/ha. Storage quality is better
than Yamuna Safed-3. Suitable for exports. The variety was notified by Government of India
in 2006 vide notification no. S.O. 597(E) dated April 25,2006.
MEDICINAL VALUE
Garlic is one of the important bulb crops grown and used as a spice or a condiment
throughout India. According to the Unani and Ayurvedic systems as practised in India, garlic
is carminative and is a gastric stimulant and thus help in digestion and absorption of food.
Allicin present in aquous extract of garlic reduces cholesterol concentration in human blood.
The inhalation of garlic oil or garlic juice has generally been recommended by doctors in case
of pulmonary tuberculosis, rheumatism, sterility, impotency, cough and red eyes. Garlic
175
possesses insecticidal action. About 1% garlic extract gives protection against mosquitoes for
8 hours. Extract of garlic along with chilli and ginger has beneficial action against soil
nematodes. Beneficial use of garlic extract has been found against many fungi.
Institutions engaged in Allium Research in India
1. Division of Vegetable Crops, Indian Agricultural Research Institute (IARI),

New Delhi - 110 012.
2. Indian Institute of Horticultural Research (IIHR), 225, Upper Palace Orchards, Bangalore
- 560 080.
3. National Horticultural Research and Development foundation (NHRDF), 2954-E, Kanda

Batata Bhavan, New Bombay, Agra Road, Nasik - 422 001, Maharashtra.
4. Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri - 413 722, Ahmednagar,

Maharashtra.
5. Tamil Nadu Agricultural University (TNAU), Coimbatore - 641 004, Tamil Nadu.
6. Punjab Agricultural University (PAU), Ludhiana - 141 004, Punjab.
7. Haryana Agricultural University (HAU), Hisar - 125 004, Haryana.
8. Rajasthan Agricultural University (RAU), Durgaoura - 302 015, Jaipur, Rajasthan.
9. Dr. Y.S. Parmar University of Horticulture and Forestry (YSPUHF), Nauni, Solan - 173
230, Himachal Pradesh.
10. Division of Crop Improvement, Vivekananda Parvatiya Krishi Anusandhan Shala

(VPKAS), Almore - 263 601, U.P.
11. National Bureau of Plant Genetic Resources (NBPGR), IARI, Campus, New Delhi.
12. JawaharLal Nehru Krishi Viswavidyalaya (JNKV), Jabalpur - 482 004, Madhya Pradesh.
13. Chandra Shekar Azad University of Agriculture and Technology (CSAU), Kalianpur,
Kanpur - 208 002, U.P.
14. G.B. Pant University of Agriculture and Technology (GBPUAT), Pantnagar - 263 145,
District Nainital, U.P.
15. Orissa University of Agriculture and Technology (OUAT), Bhubaneswar - 751 003,
Orissa.
176
Varietal status
Tamil Nadu - i) Mettupalayam Local, (Malai Poondu) and Ecotypes.
ii) Five Month Garlic.
iii) Singapore
iv) Ooty - 1 (HRS, Ooty, TNAU).
Preference : PINK varieties, Medium clove size, Shorter duration.
GUJARAT - Jamnagar - Large cloves
ANDHRA PRADESH - Fawari, Rajella Gaddi
VARIETIES RELEASED BY NHRDF (NASHIK): Agri found white (G41) - Recommended

for Maharashtra
Yamuna Safed (G1) - Recommended for North India
Agri Found Parvati (G313) - Recommended for Hills of North India.
G282 - 16 T/ha; TSS - 41.93 - Suited for export.
HG1, HG6 - Haryana Agricultural University
Questions
1. What is the chromsme number for garlic
2. Name garlic varieties Recommended for Hills of North India.
3. Name a variety suitable for Tamilnadu
4. Name garlic varieties suitable for export
5. Write about the breeding achievement in garlic
References
Kalloo, G. and B.O.Bergh.1993. Genetic improvement of vegetable crops. Elsevier Ltd

177
L-23-24
POTATO
 Botanical Name : Solanum tuberosum
 Family : Solanaceae
 Chromosome no : 2n= 4x= 48 ( Tetraploid)
 Origin : Andean mountains of Bolivia and Peru in South America .
HISTORY :
 Potato was introduced to India by Portuguese in 17 th century.
 The early history of potato in India is obscure till mid 18th century . However potato
continued to be brought in to India by British settlers and grown in backyards of
residences.
 In 1830 onwards potato was commercially grown in higher altitudes of hills in India
(Hooker,1984 and Buck,1925) from where it spread to plains of India
 Cultivated from originated from wild diploid species Solanum leptophytes and the
first domesticated species was Solanum stenotomum.
SPECIES OF POTATO
 Solanum demissum: 2n=72(Resistant to late blight)
 Solanum antiporizii: 2n=48(Resistant to late blight)
 Solanum acaula: 2n=48(Resistant to frost)
 Solanum curtilobum : 2n =24(Resistant to frost)
 Solanum rybinii: 2n=24(resistant to certain viruses and it is a non dormant type)
 Solanum phureja: 2n=24( develop tubers in comparatively hotter regions)
 Solanum chacoense: 2n=24(develop tubers in comparatively hotter region)
Others Species :
 Solanum infundibuliforme : Drought Resistant.
 Solanum jamesii : Resistant to Alternaria leaf spot and

Phytophthora.
 Solanum fendleri : Can be hybridized with wild and cultivated potatoes

178
Botany
Habit :
 Potato is a much branched. The plants are erect when young and thus grow prostrate
to the ground because of leaf form. The above ground shoots are called ‗haulms‘.
Inflorescence :
 It is a monochasial cyme (cymose inflorescence developed in single system of
branching) .
 Flowers are white. Petals are white /cream in colour
Stamens :
 Five in number ( yellow to orange in colour). Anthers are erect and longer than
the filaments with apical dehiscence.
Ovary :
 Ovary is superior ,bicarpillary , style is simple and erect capitates. Stigma is

capitate.
Fruit :
 Fruit is a berry with numerous small seeds. Potato fruit is also called as potato
ball (or) Potato apple (or) Seed apple.
Floral biology
 Anthesis time and closing of flowers varies with varieties and species. Pollen
production is abundant from early morning to 10am.
 Production of fertile pollen is more in diploids.
 Stigma receptivity and anther dehiscence also takes place at the same time.
POLLINATION AND FERTILIZATION:
• Self pollination occurs.
• Pollination takes place either by direct contact of the anthers with the stigma or
through insect, mostly bumble bees.
179
Flower
Variations in flowers of different species of potato
PLANT MORPHOLOGY
ROOTS:
Potato plant may develop from seed or tubers .
Plants grown from seed form a slender tap root with lateral branches.
Plants grown from tubers form adventitious roots at the base of each sprout and, later above
the nodes of the underground part of each stem.
Occasionally, roots may also grow on stolons. In comparison with other crop, the potato root
system is weak. Therefore good soil condition is necessary for potato growing
180
Plants grown from tubers form adventitious roots at the base of each sprout
STOLONS: Morphologically, potato stolons are lateral stems which grow horizontally below
ground from buds of the underground part of stems .stolon may eventually form tubers by
enlargement of their terminal end.
The potato stem system consists of stems, stolons and tubers.
TUBERS: Morphologically, tubers are modified stems and constitute the main storage
organs of the potato plant. The eye of the potato tuber morphologically corresponds to the
nodes of stem. Sprouts grow from the buds in the eyes of a tuber. The color of the sprout is a
distinguishing varietal character.
Stem:
 Stem is generally solid , ribbed or smooth .
Leaves :
 Leaves are compound, pinnate, 10-20 cm long alternately arranged.Terminal

leaflets are larger in most varieties and therefore the shoots lie prostrate to the
ground.
181
PLANT MORPHOLOGY
GROWTH HABIT (herbaceous)
*The growth habit of the potato varies within and between species
Breeding objectives :
1. Breeding for high yield
Yield of tubers is decided by number of tubers and tuber
2. Breeding for varieties having better morphology of tuber
Better morphology of tuber is determined by
a) Eye depth
b) Flesh colour
c) Growth cracks
d) Hollow heart
e) Shape
f) Skin colour
3. Breeding for better quality:
Depends on many factors
a) After cooking blackening
b) Dry matter.
182
c) Enzyme browning.
d) Glycoalkaloid level
e) Reducing sugar content
f) Storage properties
4. Breeding for disease resistance
Early blight, late blight, powdery scab., verticillium wilt, virus diseases.
Resistant source : S.demissum, S.acaule ssp. andigena
5. Breeding for pest resistance
Nematode is the major pest
ssp.andigena - tolerant.
S.verineii resistant to Aphids, Colorado beetle.
GENETIC RESOURCES:
Potato germplasm at CPRI
Ssp andigena :872-from 7 countries
Ssp tuberosum :854- from 32 countries
Wild semiculture sp :250-from 4 countries
Source of resistance
Bacterial wilt : S.microdontum
PVX, PVY : S.acaule
Potato tuber moth : CP 1824,CP 1832
Aphids :S.berthaulti
Group tuberosum germplasm has narrow genetic base because
They are descedent from a very small number of introduction of andegena potato
Blight epidemic of the 1840' s reduces greatly amount of variation present in potato
Group andegena : short adopted and ancestral form of tuberosum has wide variability
183
Resistant / tolerant –biotic and abiotic stress

High starch and protein content .good keeping quality
Responsiveness to fertility applied .
BREEDING METHODS :
1. Introduction :
Earliar varieties viz., Magnum Bonum, Craigs defiance and Up-to-date

Secondary introduction: Hybrid DN-45-Katahdin × president
Kufri kisan is a multicross involving Ekishrozn from japan
2. Clonal selection:
Kufri red from Darjeeling Red Round K-1241 is selection from phulwa.
3. Hybridization and selection :
Flowering problem at Kufri

Kufri Jyothi :selection from A-306× A2814
Kufri Kundan :selection from Ekishran× Katahdin
4. Heterosis breeding:
Heterosis is observed for earliness, tuber size, tuber weight

F1 seed production by hand emasculation and pollination after identification of
good specific combination. Pollen sterility is common.
Inbreeding depression is more. Seed set is poor. Not exploited
Emasculation and Pollination in potato

184
5. Polyploidy breeding:
Potato is basically a polyploid

Haploids -teraploid×diploid
Different species are with different ploidy level
For, resistance breeding , ploidy breeding is must
6. Bridging Species:
 KufriKuber -(S. curtilobum x S. tuberosum) x S. andigenum) (Resistant to Potato leaf

roll )
 A species used in gene transfer from one species to another sexually incompatible
species, bridging species is compatible with both donor and recepients species, it may
be a natural or a synthetic species.
Interspecific hybridization :
1. S. demissum × S. tuberosum
2n=72 2n=48
F1 sterial (univalents)
Bridge species: S .demissum × S. ryberoum
F 1 (fertiel) ×S.tuberosum
Fertile progenies
2. S. acaule × S. tuberosum
F1 sterial (univalents)
S. acaule × S.simplicifolium
F 1 (fertiel) ×S.tuberosum
Fertile progenies
Breeding strategy to obtain 4x hybrid from 4x-2 crosses.
Haploid tuberosum /andegena Cultivated /wild species
(2n=2x=24) female fertility (2n=2x=24) new

adaption genes/alleles
185
Agronomic superior cultivar x

Haploid-species hybrid
(2n= 2x= 48) (2n=2x=24)
Unrelated to haploid
Diplandrous tetraploid hybrid

(2n=4x=48)
Heterosis /allelic diversity
Desirable combination of traits
7. Mutation breeding :
Kufri sindhu (+LB 120 ays), Kufri Chandramukhi(95 days),
Kufri Alankur , Kufri Chamatkar, Kufri Jeevan, Kufri Naveen
Kufri Jyoti, Kufri Moti, Kufri Lauvkar, Kufri Dewa
Kufri Sheetman: (+ Frost )
Hybrid 2236 did well at short and day conditions
HB827, HB841 and HB 858 suited for high temperature
Kufri Badshah : Horizontal resistance to LB
JW 160 -Tolerant to late blight with inter adaptability
H92/621: Tolerant to heat and drought and resistant to mites and leaf hopper
Breeding achivements
 Kufri Sindhuri - Resistant to Lateblight Duration 90 days
 Kufri Swarna - Resistant to golden cyst Nematode.
 Kufri Badshah- Resistant to Potato virus X
 HB821, HB841,HB 858 – Suited to High Temperature.
 JW 160 - Tolerant to late blight
 H92 / 621 - Tolerant to heat and drought and Resistant to mites and Leaf hopper .
BIO TECHNOLOGY:
186
• Potato was ideal material for application of biotechnological method such as anther
culture, somatic hybridization, genetic information , in vitro, ,somaclonal variation
selection and production of transgenic.
• The potato has many pest and pathogens that can reduce yield and overall plant vigor.
The Colorado potato beetle (Leptinotarsa decemlineata) is high destructive pest of
potato in North central and Asia.
• Defoliation by adults and larvae can reduces yield and even result in total tuber loss.
Despite breeding effort, no potato cultivars with demonstrated resistance to Colorado
potato beetle have been released commercially.
• The bacterium Bacillus thuringiensis (Bt) ssp.tenebrionis Berliner, produces a cry 3A

protein that has toxic effects on coleopteran, including Colorado potato beetle.
• The choice of a Bt gene for engineering host plant resistance has multiple advantages.
Bt protein have very specific modes of action.
• Such that a protein with specific toxicity towards coleoptera would not be toxic to
humans.
Crop improvement:
Varieties Breeding Specific features

method
New varieties
Kufri Himalini - Moderate resistant to late blight
Kufri Shaillja - Moderate resistant to late blight
Kufri Girdhari - Highly resistant to late blight
Kufri Himsona - Highly resistant to late blight
Kufri Jyoti Large white oval tubers with white flesh;

resistant to early and late blight. It has an yield
potential of 25 t/ ha in a crop duration of 90-95
days. Suitable for hills in North India.
Early varieties (80-90 days)
Kufri Seedling 4485 x Tubers are oval in shape with white skin.
Chandramukhi Kufri Kuber Suitable for North Indian plains and plateau.
Duration is 90-100 days. It yields 25-27 t/ ha.
Kufri Jawahar Kufri Medium, round-oval, creamy white, fleet eyes

Neelamani X and pale yellow flesh. Crop matures in 80-90
187
Kufri Jyothi days. Average yield is 40 t/ha. Moderately

resistant to late blight. Suitable in intensive-
cropping system
Kufri Laukar Adina X Sarkov Large, round, white, fleet eyes and white flesh.
Able to build up yields rapidly under warmer
climate. Crop matures in 75-80 days. Average
yield is 30 t/ha.
Kufri Sheetman Craigs defiance Resistant To Frost

x phulwa
Kufri Khyati - -
Kufri Surya Kufri Laukar x Heat tolerant variety, field resistant to leaf
LT-1 hoppers
Kufri Ashoka EM/C-1020 x Large, oval-long, white, fleet eyes and white
Allerfruheste flesh. Susceptible to late light. Crop matures in
Gelbe 70-80 days, Average yield is 40 t/ha.
Medium varieties (90-100 days)
Kufri Bahar Kufri Red It has large sized tubers of round to oval
×Kufri Gineke shape. The sprouts will be red in colour. The
skin as well as flesh are white. Though it has a
better yield potential than Kufri Chandra
Mukhi, it is susceptible to major diseases.
Kufri Sutlej Kufri Bahr x Moderately resistant to late blight

Kufri Alankar
Kufri Anand PJ-376 PH/f- Resistant to late blight, tolerant to frost

1430
Kufri Arun Kufri Lalima x Field resistant to late blight; tolerant to frost
MS/82-797
Kufri Pukhraj Craig ' defiance Moderately resistant to late blight

X JEX/B-687
Kufri Pushkar QB/A 9-120 x Field resistant to late blight, moderately

Spatz resistant to phoma and early blight
Kufri Lalima Kufri Red x AG Moderately resistant to early blight and resistant
14 (Wis. X 37) to PVY
Kufri Kanchan SLB/Z-405(a) x Field resistant to late blight

Pimpernel
188
Kufri Chamatkar It possesses round medium sized tubers with

white skin and pale yellow flesh. It is
susceptible to a number of diseases. The
duration is 110-115 days.
Kufri Muthu It has large round to oval shaped tubers with

white skin and flesh. It is suitable to grow in
Nilgiris. The duration is about 100-110 days.
Late varieties (100-110 days)
Kufri Sindhuri Kufri Red x Suitable for dehydrated flakes and canning. It
Kufri Kundan has an yield potential of 35-40 t/ ha in 120 -125
days. The tubers are red in colour hence called
―Sindhuri‖, and round in shape. It is fairly
tolerant to frost.
Kufri Badshah Kufri Jyoti x Moderate resistant to late blight ,early blight an
Kufri Alankar potato virus X
Kufri Dewa It is tolerant to frost and tuber rotting. The

tubers are brownish white with pink tinge near
the eyes. The flesh is white in colour. It is
commonly grown in Uttar Pradesh and Bihar.
Kufri Jeevan The tubers are oval in shape with white skin.
The flesh is pale yellow. It has an yield
potential of 40 t/ ha in 125-130 days. It is
commonly cultivated in Himachal Pradesh.
Processing Variety
Kufri Frysona MP/92-30 x Suitable For Processing To

MP/90-94 French Fries
Kufri Chipsona - MP/91-86 x Resistant To Late Blight, medium Maturing

3 Kufri Chipsona- Variety
2
Kufri Chipsona - MEX.750826 x Chips and flakes

1 MS/78-79
Kufri Chipsona - F-6 x QB/B 92- Chips and flakes

2 4
Kufri Chipsona-4 Atlantic x Early Maturing Resistance To Late Blight

MP/92-35
Kufri Surya Kufri Lauvkar x Heat Tolerant Variety Field Resistance To Leaf
LT-1 Hoppers
189
Kufri Alankar Kufri Anand Kufri Arun
Kufri Ashoka Kufri Badshah Kufri Bahar
Kufri Chamtakar Kufri Chandramukhi Kufri Chipsona 1
KufriChipsona 2 KufriChipsona 3 KufriChipsona 4

190
KufriDewa KufriKuber KufriKuber
Kufri Frysona Kufri Kundan Kufri Lalima
Kufri Laukar Kufri Sheetman Kufri Surya
Kufri Sutlej Kufri Sindhuri Kufri Red

191
Breeding for Biotic Stress
Late blight of Potato S. bulbocastanum , S. demissum , S. stoloniferum , S. sucrense,

S. Verrucosum , , S. chacoense , S. pinnatisectum ,
S.microdontum
Wart S. chacoense , S. sparsifolium , S. spegazzinii

Charcoal rot S. chacoense , S. sparsifolium
Bacterial diseases S.microdontum
Insect pest(Aphids) S. berthaulti
Viral diseases (Potato virus x S. chacoense , S. acaule , S. stoloniferum , S.microdontum
and y)
Potato cyst Nematode S. chacoense , S. sparsifolium
Root Knot nematode S.microdontum , S. vernei , S. spegazzinii
Questions
1. Write down the related species of potato
2. Write down the resistnace sources of potato against biotic stresses
3. Write down the breeding objectives
4. Write in detail about the crop improvement in potato
5. Name some varieties suitable for chips making
References
Bradeen, J.M. and C. Kole . 2011. Genetics, Genomics and breeding of potato.
CRC, Press, Science Publishers, Hampshire.
192
L-25
CASSAVA (TAPIOCA)
(Syn: Tapioca) Manihot esculenta (Crantz)

(2n = 2x = 36)
(Hindi: Mravuli)
Family: Euphorbiaceae
Cassava is the most important starchy root crop grown in the tropics and is mainly cultivated
in southern peninsular India. Introduced during seventeenth century by Portuguese, the crop
played a significant role to overcome food shortage among the low income group of people in
Kerala. Underground tuber is rich in starch and mainly consumed after cooking. Processed
products like chips, sago and vermicelli made of tapioca are also popular in the country.
Being easily digestible, it forms an important ingredient in poultry and cattle-feeds. It is also
widely used for production of industrial alcohol, starch and glucose.
Origin and distribution
Cassava is not known in wild state. North-Eastern Brazil is the centre of origin.
Portuguese distributed the crop from Brazil to countries like Indonesia, Singapore, Malaysia
and India. Nigeria is the major growing country in world accounting for 50% of area and
production. In India crop is cultivated in southern peninsular region, particularly Kerala,
Tamil Nadu and Andhra Pradesh contributing 93% of area and 98% of production in the
country. Kerala accounts for nearly 50% of total area under cassava in India and is mainly
grown as rainfed crop.
Botany
Cassava belongs to family Euphorbiaceae and is diploid (2n=36). Polyploids with
2n=54 and 72 are also available. It is a perennial shrub producing 5-10 cylindrical tubers per
plant. Being a member of family Euphorbiaceae, it produces latex. The stem is woody and
variously branched. Two distinct types are present – one without branching at the top and the
other with spreading nature. Leaves are palmately lobed with 5-9 lobes. Cassava is
monoecious in nature and cross-pollinated. Female flowers are a few in numbers and are
borne in the base of inflorescence and male flowers are borne above. Female flowers open
about 10 days before male flower anthesis. Stigma is receptive from 6.30 a.m. and continues
up to 2.30 p.m.
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Plants when raised from seeds produce typical tap root system. Since crop is mainly
propagated by vegetative means by stem cuttings, numerous adventitious roots develop, of
which a few develop into tubers. Tubers are composed of a thin peridium, white a few
develop into tubers. Tubers are composed of a thin peridium, white or purple cortex known
as rind and central massive flesh rich in starch (25-40%). Bitterness often encountered in a
few varieties and at certain stage is due to a bitter principle cyanogenic glucoside (HCN).
Varieties
Improved varieties developed at Central Tuber Crops Research Institute,

Thiruvananthapuram are as follows:
H 97
It was developed by crossing Manjuvella and Brazilian seedling selection. It has an

yield potential of 25-30 t/ ha in 9½-10 months duration. The tubers have 29 per cent starch.
This variety is tolerant to cassava mosaic disease and drought.
H 226
It is a cross between Ethakka Karuppan x M4. The tubers have sweet flesh encased in
light purple rind and possess good cooking quality. Tubers contain 28 per cent starch. It
comes to maturity in 9½-10 months and yields 30-35 t/ ha.
Sree Visakham (H 1687)
It was developed by crossing AC No. 1501 x S 2313 (Madagascar). The plants are
erect and sparsely branched. Tubers are non-bitter with 28 per cent starch content. The tubers
mature in 10 months. This variety has an yield potential of 33-38 t/ ha.
Sree Sahya (H 2304)

194
It was developed from a multiple cross. Plants are non branching, tubers are medium
in size, non-bitter; starch 28 per cent. Tubers have neck. It yields 35-40 t/ ha in a crop
duration of 10-10½ months.
Sree Prakash (S 856)
It is a selection from a local accession. It yields 35-40 t in 7½ months. The tubers

have good culinary qualities with 30 per cent starch. The mature stem is pinkish with purple
petiole. It is resistant to Cercospora leaf spot and tolerant to drought.
Sree Harsha
This variety was developed at CTCRI, Thiruvananthapuram. It is an improved version

of Sree Sahya (H2304) with plants having top branching. The average yield of tuber is 35 to
40 t/ ha; starch content is 38 per cent. It is an ideal variety for industrial use and poultry feed.
At Tamil Nadu Agricultural University, Coimbatore varieties with better adaptability

to Tamil Nadu conditions have been developed.
CO 1 (ME 7)
It is a clonal selection from a local type collected from Tiruchi District. The Tubers
have whitish brown skin with a cream rind and white flesh. It has an yield potential of 29-30
t/ ha in a crop duration of 8½ -9 months. But it is susceptible to cassava Mosaic Disease.
CO 2 (ME 167)
It is an open pollinated seedling progeny raised from the seeds collected from a
Thiruvarur type of Tanjore District. It is a branching type with compactly arranged tubers
having brown skin, creamy white rind and creamy white flesh. It is suitable for consumption
as well as starch industry. It yields 35-37 tonnes/ha. in crop duration of 8½ months.
CO 3 (ME 120-1)
It was developed at TNAU by selection from OP seeds received from IITA, Ibadan,
Nigeria. Since the plants are heavily branching, periodical removal of sprouts is necessary.
In a crop duration of 8½ months it can yield 42 t/ ha. Outer skin is dark brown with yellowish
195
white rind and white flesh. It is resistant to Cassava Mosaic Disease. Tubers have 35.6 per
cent starch.
MVD 1 (S2371)
This was developed at Tapioca Experiment Station, Mulluvadi of Salem district in

Tamil Nadu. It has a yield potential of 34 t/ ha in 9 months. It is rich in starch (35.6%).
Questions
1. Name the Tapioca varieties developed from TNAU
2. Name the variety released from Tapioca Experiment Station
3. Name some varieties released from CTCRI, Thiruvananthapuram
4. What is the centre of origin for Tapioca?
5. What is the system of breeding of tapioca?
References
1. Kalloo, G. and B.O.Bergh.1993. Genetic improvement of vegetable crops. Elsevier Ltd
2. Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of

Vegetables, Revised, ICAR, New Delhi.
196
L-26
Sweet potato
Ipomoea batatas L.
(Hindi: Shakarkand, Mitha alu)
Convolvulaceae
2n = 90
Sweet potato is cultivated for its sweet root tubers. It is mainly used for human food
after boiling or steaming, baking or frying and also as animal feed. Since roots contain 16%
starch and 4% sugar, it is sued for production of industrial starch, syrup and alcohol.
Sweet potato is a native of tropical America. It is an important tuber crop in tropical and sub-
tropical countries like Africa, China and India. In India, it is grown mainly in Andhra
Pradesh, Assam, Bihar, Tamil Nadu and Orissa.
Botany
Sweet potato is a hexaploid species with 90 somatic chromosomes. It is a perennial herb with
trailing vines and with a cluster of a few medium sized tubers. For cultivation purpose, it is
treated as an annual with duration of 90 to 120 days. Though plants produce viable seeds,
highly heterozygous nature of the crop results in a heterogeneous population. Hence stem
cuttings are used for propagation purpose.Sweet potato has an extensive fibrous root system
both at stem cuttings and at nodes touching soil. Some roots act as storage organs for storing
reserve food. Storage roots may be fusiform, spindle or globular in shape and surface is
smooth. Skin has white, red or light copper colour. Flesh may be white or with different
combinations of orange and red.Leaves are simple, alternate and stipulate. They vary in size
and shape, occasionally in same plant. Shape varies from ovate to cordate, hastate or deeply
lobed and may change on ageing. Leaf shape is an important character for identifying
clones.All clones do not flower and in flowering ones, duration and initiation of flowering
vary. Flowers are axillary and borne solitary or in simple cymes. Flowers are bisexual.
Corolla is attractive and funnel shaped formed by fusion of five petals. Anthesis starts before
dawn and closes by 9-11 a.m. Pollination is entomophilies. Fruit is a capsule with false
septa. Seed coat is hard and impervious to water. Hence, scarification is required for
promoting germination.
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Varieties
Pusa Safed
It was developed at IARI. Tubers are long white, less affected by weevil. It yields 26-
30 t/ ha in 105-120 days.
Pusa Lal
Tubers are long and pinkish red. It yields 20 - 22 t/ ha in 120 days.
Kalmegh
The tubers are round, light brown. It has an yield potential of 26 - 32 t/ ha in a crop
duration of 90-105 days.
CO 1 (IB 3)
It is a clonal selection developed at TNAU, Coimbatore. It is moderately vigorous and

less spreading. The tubers are with light pink skin and white flesh and have a starch content
of 24.2 per cent. It has an yield potential of 28 t/ ha in a crop duration of 135 days.
CO 2 (IB 81)
It is a seedling clone obtained from open pollinated seeds of IB 37 in the germplasm

maintained at Faculty of Horticulture, Tamil Nadu, Agricultural University, Coimbatore. The
tubers are medium sized with light pink skin and white flesh. Tubers are rich in starch (29.5
%). It has an yield potential of 32 t/ha in a crop duration of 110-115 days.
CO 3 (IB 2837)
It is a seedling clone obtained from the seeds of random mating population of IB 75.
The tubers are medium sized with light red skin and orange flesh indicating high carotene
content (13.28 mg/100 g). The starch content is 30.7 per cent and the sugar content is 2.9 per
cent. It has an yield potential of 43.6 t/ha in a crop duration of 105 days.
Varieties which were developed at CTCRI, Thiruvananthapuram are as follows;
H 41
198
It was evolved by crossing Norin (Japanese types) with a local type. It is a semi
spreading type with fusiform tubers having reddish purple skin and white flesh.
It possesses good culinary quality. It yields 20-25 t. ha in a crop duration of 120-125 days.
H 42
It was developed from a cross Kelladumph (indigenous variety) x Triumph (American

variety). Purple coloured tubers have medium thick pink rind and cream flesh. It has an yield
potential of 20-25 t/ ha in 120 - 125 days.
Sree Nandini (76-OP-217)
It is a selection from the open pollinated progenies of the mother parent S 32 from
CTCRI germplasm. It has a semi-spreading plant type with simple entire leaves. Emerging
leaves are light green in colour, tuber skin light cream and flesh white in colour. It has a
good culinary quality. The potential yield is 20-25 t/ ha in 100-105 days.
Sree Vardhini (76-OP-219)
It is an open pollinated seedling selection of the mother plant S 13. It is a semi

spreading type with shallow lobed leaves; the colour of emerging leaf is light brown. Three to
four tubers are produced per vine. The skin colour of the tuber is light pink while the flesh is
light orange in colour indicating high carotene content (1200 IU/g). It has an yield potential
of 20-25 t/ ha in 100-115 days.
Sree Bhadra
Released by CTCRI, Thiruvananthapuram through introduction and evaluation from

IITA, Nigeria; vines are semi-spreading, greenish brown with broad cordate leaves. Tubers
199
are spherical with pink skin and creamy flesh. It yields 25-27 t/ ha. The starch content of the
tubers is 20.2 per cent and carotene content is 972 IU/ g.
Sree Retna
Another variety developed at CTCRI, Thiruvananthapuram from a cross S187 x Sree

Vardhini. The vines are greenish brown in colour. Tubers are spherical with purple skin and
orange flesh. The starch content of the tubers is 23 per cent while the carotene content is
3578IU/ g.
Varsha
It is a high yielding variety of sweet potato. Hybrid, Semi spreading type. Drought
tolerant.Released for konkan region of Maharashtra. Duration 120 days. Yield 17-22 T / Ha.
Questions
1. Name a sweet potato variety developed from TNAU
2. Name some varieties develped from IARI
3. Name some varieties develped from CTCRI

200
4. Name a hybrid of Sweet potato
5. What is the mode of poliination in sweet potato
References
Kalloo, G. and B.O.Bergh.1993. Genetic improvement of vegetable crops. Elsevier Ltd

L-27-28
Bhendi (Okra)
Abelmoschus esculentus (L.) Moench
Malvaceae (2n=72)
Okra originated in tropical and subtropical Africa. Existence of a large number of related
species with wide variability and dominant characters suggest possible role of India as a secondary
centre of origin.
India is the largest producer of okra in the world. It is also used as a vegetable in Brazil, West
Africa and many other countries. In India, major okra growing states are Uttar Pradesh, Bihar
and West Bengal.
Breeding objectives
1. To develop high yielding varieties with dark green, tender, thin, medium long smooth 4-
ridged pods.
2. To evolve varieties resistant to YVM, fungal diseases like fusarium wilt, cercospora leaf
spot, powdery mildew, alternaria leaf spot, fruit rot and anthracnose.
3. To combine resistance to YVM virus with resistance to fruit and shoot borer, white fly,
jassids and root knot nematodes.
4. Suitable ideotype; short plants with more number of nodes with short or, internodal
length; easy snap of fruits from the stalk.
5. Varieties tolerant to abiotic stress especially tolerance to low temperature, excessive rains
saline and alkaline soil.
6. To develop varieties suitable for export market and for processing industry.
Genetic Resources
The major collection is maintained by the NBPGR, New Delhi. IBPGR has
designated NBPGR with global responsibility of base collection of okra. Okra germplasm
endemic to India have been given emphasis for collection and evaluation. The prime object
has been to identify sources of resistance to YVM virus.
Cytogenetics
Successful intra specific hybridization in the species Abelmoschus esculentus (L)
Moench has helped to generate considerable variability in isolating desirable genotypes.
201
There are 30 species under the genus Abelmoschus in the old world and few species
in the new world . A. esculentus is the only species known to be cultivated. Now, it has been
known that A. manihot spp. manihot is also cultivated on a limited scale in the African
Continent.
However, barriers in the interspecific hybrids have restricted the progress to transfer useful
genes from the wild species to the cultivated ones. Cultivated okra (A. esculentus) is
polyploid in nature (2n = 130) .
Interspecific hybrids between A.esculentus (n = 65) with 3 wild species viz.,
A. tetraphyllus Roxle (n = 69), A. manihot Medik (n = 33) and A. manihot L Medik sp.
Manihot (n = 97) were studied . From the pollen sterility, which is one of the isolating
mechanisms, A. tetraphyllus appears to be more closely related to A.esculentus as compared
to two other wild species.
Taxonomy and botany
Cultivated bhendi belonging to Malvaceae was earlier placed under Hibiscus
esculentus L. Since its calyx, corolla and staminal column are fused together and fall down at
anthesis (caducous), it was renamed as Abelmoschus esculentus L.since in Hibiscus, calyx is
persistent. Cultivated bhendi is an annual herb with duration of 90-100 days. Flowers are
bisexual and often cross-pollinated. Time of anthesis is 8.00-10.00 a.m. Dehiscence of
anthers occurs 15-20 minutes after anthesis and is completed in 5-10 minutes. Pollen fertility
is maximum in the period between one hour before and after opening of flower. It takes 2 to 6
hours for fertilization after pollination. Stigma is receptive at opening of flower and hence,
bud pollination is not effective in okra. Fruit is a capsule. Usually fibre development starts
from fifth to sixth day.
Most of cultivated varieties are amphidiploids with 2n=130. A. esculentus is noted for
its chromosome polymorphism and 2n ranges from 72 to 144. It tolerates addition or deletion
of one or a few chromosomes. The chromosome number of A .esculentus is 2n=130 which is
evolved by crossing. A. tuberculatus (2n=58) with A. ficulneus (2n=72). The F1 developed
was subjected to colchicine treatment to make it an amphidiploid. A. esculentus (2n=130).
Similarly, A. caillei, a complex polyploid, was evolved by colchicines treatment of F1 of
cross between A. manihot and A. esculentus.
Four species viz., A. esculentus, A. manihot, A. caillei and A. moschatus include both
cultivated and wild forms.
Various Species of bhendi
Abelmoschus Somatic Wild(w)/Cultivated(c) Distribution
species Chromosome
number
A. angulosus 56 W India and
Srilanka
A.aberculatus 58 W India
A.moschatus 72 WC India, Nepal
and Srilanka
A.ficulneus 72 W India and
Bangaladesh
A.tetraphyllus 138 W India, Nepal
and Srilanka
A.tetraphyllus 138 W India, Nepal
var.pungent and Srilanka
A.crinitus 9 W India and
Srilanka
202
A.callei 196 W India

A.manihot 66 WC India and
Srilanka
A.esculentus 130 C worldwide
A.moschatus A. manihot
.
BREEDING METHODS
Pure-line Selection:
 This is applicable to landraces/cultivars collected from Farmers‘ field, for example,
PusaMakhmali was bred from a material collected from West Bengal. Similarly, Co 1
is a single plant selection from Red Wonder.
Pedigree Method:
 This method is applicable to the segregating generations after hybridization between
desirable promising donors.
 The individual plant selection starts in the F2 generation and continues till F5 or F6.
 For example, Pusa Sawani was developed through this method in an inter-varietal
cross. Punjab Padmini, ParbhaniKranti, P7, Arka Anamika and Arka Abhay are
examples of pedigree selection following interspecific hybridization.
Mutation breeding
 MDU 1 is an Induced Mutant from Pusa Sawani
 Punjab 8 - It is an induced mutant derived from Pusa Sawani treated with 1% EMS.
Heterosis breeding
 Heterosis in okra has been reported for various economic traits, viz. early and late
flowering, plant height, number, weight and size of pods, number of ridges,
marketable and total yield.
 Using hand emasculation and pollination, commercial hybrids are developed. There
are promising hybrids under private sector seed companies in India. The current seed
market of okra in India is approximately 4000 tons for open-pollinated cultivars and
1000 tons for hybrids.
Crop improvement
Yellow vein mosaic virus disease being a serious problem in okra cultivation,
concerted effort was made to develop high yielding as well as YVMV resistant varieties in
the country. With development of YVMV resistant variety, Pusa Sawani, most of the
primitive low yielding local cultivars has become less significant. After break down of
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resistance of Pusa Sawani, research on virus resistance was intensified at various research
centres in India and it resulted in development of a number of YVMV resistant varieties.
Identified promising lines of okra for various attributes
Early flowering IC-128062, 11479,117218, EC-325356,
329370
Long fruiting duration IC-264697, 2647, EC-305749, 306741
Resistant to damping off, Rhizoctonia Red Ghana, Sel-7-1, BH-27, IC12096
solani
Resistant to powdery mildew Nigeria, EC32598, IC8248,
A.tetraphyllus, A.angulosus
Resistant to Cercospora blight Sel-7-1, Round selection, EC-32598,
A.crinitus, A.moschatus A.angulosus
Resistant to Fusarium wilt IS-9273, Pusa Sawani, Pusa Makhmali
Resistant to leaf curl virus A.ficulnes, A.manihot
Resistant to microphomina IC-90186, U-43087, U4365
Resistant to Yellow vein mosaic virus A.manihot, Parbhani Kranti, Punjab
Padmini, IC-1542, ACC-49, NIC-9303A
Resistant to Jassid IC194, Sesswal Local, IIHR21, AE30
Crimson Smooth
Resistant to fruitborer A.tuberculatus, Red -1, Pusa Sawani,
Long green
Resistant to nematode Long green smooth
Tolerant to mite A.angulosus
Varieties
Pusa Sawani
Developed at IARI - fruits - bright green, medium length, 5 ridges; It has got an yield
potential of 8-10 t/ ha in a crop during of 90 - 95 days. This variety can be grown in Kharif
and Rabi. Not suited for summer as it is susceptible to YVM.
Pusa Sawani
MDU 1
Developed at AC & RI Madurai by gamma irradiation of seeds of ‗Pusa Sawani‘.
Fruits - light green with long stylar end. Plants - compact with close arrangement of nodes
also susceptible to YVM. Yield: 10 - 11 t/ ha.
CO1
It is a pure line selection from Hyderabad ‗Red Wonder‘. Fruits - pinkish red. Yield:
12 t/ ha; susceptible to YVM; not very popular among farmers.
Punjab Padmini
Evolved from PAU - Ludhiana, fruits - dark green - 20 g each. It tolerates yellow
vein mosaic to certain extent under field condition.
204
CO 2
F1 hybrid (AE 180 x Pusa Sawani), developed at Department of Horticulture, TNAU.
Fruits - very long - 22 - 25 cm, 7 - 8 ridges and light green. Highly susceptible to YVM and
hence cannot be recommended for summer - suitable for dehydration.
Parbhani Kranti
Marathwada Agricultural University, Parbhani - Maharashtra. Field tolerant to YVM
(more than 5%) recomended for growing during summer season.
Fruits: dark green in colour. Yield: 10 - 12 t/ ha in kharif and 7-8 t/ ha in summer
season. Evolved by back cross method using a wild relative called Abelmoschus manihot.
Arka Abhay (IIHR Sel. 4)
IIHR - Inter specific hybridization using Abelmoschus manihot ssp tetraphyllus
variety etraphyllus as a source of resistance and it exhibits a very high degree of resistant to
YVM. Yield: 10 - 12 t/ ha (summer); 16 - 18 t/ ha.
Fruits - short, dark green in colour and fetch a primium price in the market in summer
season. Duration: 120 - 135 days.
Arka Anamika (IIHR Sel.10)
This is yet another variety developed at IIHR by interspecific hybrid as before. It
shows a high degree of resistance to YVM disease and hence suitable for growing in summer.
Fruits - dark green and lengthier than Arka Abhay. Yield: 12 - 13 t/ ha (Summer); 16 - 18 t/
ha (Kharif); duration: 120 - 135 days.
CO 3 (Hybrid 8)
It is an F1 hybrid of Parbhani Kranti and MDU 1 rlesed from TNAU and has an yield
potential of 16 - 18 t/ha. It is moderately resistant to YVM. Suitable to kharif and summer
season.
Pusa Makhmali:
 It was developed by Plant Introduction Division, IARI, New Delhi, as a result of
selection from the local material collected from West Bengal. It is an early variety.
205
 Pods are smooth, straight, 5-edged, attractive, light green, slender, 15-20 cm long.
The yield potential is 100 q/ha.
Pusa A 4:
 This variety has been released by IARI in 1994 as a substitute for Pusa Sawani. The
plants are dark-green with sparse pigmentation (occasional) on stem and petiole, with
usually single stem having short internodes (2-4 cm). The leaves are broad and
medium lobed.
 The fruits are 5-ridged, attractive dark-green, 12-15 cm long having excellent shelf-
life. It is resistant to YVMV and tolerant to jassids and shoot and fruit borer.
 Green fruit yield during summer season ranges 10-12 tonnes/ ha while during kharif
and late kharif it could give still higher yields. It also responds to pruning to extend
the summer crop for added harvests during kharif season.
Kashi Mohini (VRO 3):
 Developed by IIVR, Varanasi, 110-140 cm tall plant, flowers at 4-5th node during
summer and 5-7th node during rainy season, fruits five ridged, 11 -12 cm long pods,
130-150 q/ha pod yield, tolerant to YVMV under field conditions.
Kashi Pragati (VRO 6):
 Developed by IIVR, Varanasi, 130-175 cm tall plants with average of 2 effective
branches/ plant, flowering at 4th node after 36-38 days after sowing in rainy season,
8-10 cm long pods, 23-25 pods/plant, 150-180 q/ha yield, highly tolerant to YVMO.
Varsha Uphar (HRB 9-2):
 This variety has been developed by Haryana Agricultural University, Hisar from the
cross, Lam Selection 1 x Parbhani Kranti following pedigree selection method. It was
released in 1992 and notified in 1995 by the Central Sub-Committee on Crop
Standards.
 It has resistance to YVMV and field tolerance to leaf hopper and suits to disease
prone rainy as well as disease-free spring- summer season.
 Plants are medium tall (90-120 cm) with short internodes, producing 2-3 branches
each. Petiole is pigmented.
 It takes 40 days to first flowering and 50 days to first picking. Fruit bearing starts
from 4th node. Fruits are smooth, dark-green, attractive with long tapering tip and
measure 18-20 cm on full maturity.
 Average number of seeds per fruit is 55-60. It is a prolific bearer with an average fruit
yield of 10 tonnes/ha.
Hisar Unnat (HRB 55):
 Developed by Haryana Agricultural University, Hisar from the cross, Se.1 2-2 x
ParbhaniKranti, has been released by the Central Variety Release Committee and
notified in 1996. It is resistant to YVMV, early (first picking in 46-47 days) and high
yielding (12-13 tonnes/ha green fruits) variety.
 Plants are medium tall with short internodes producing 3-4 branches each. Foliage is
green, petioles occasionally pigmented.
 Petal base is pigmented on inner side only. Fruits are green, attractive, 5 ridged and
measure 15-16 cm in length on full maturity.
 It is suitable for growing during summer as well as rainy season.
206
Questions
1. Write down the breeding objectives for okra

2. Name some varieties developed from IIVR, Varanasi
3. Name some varieties resistant to YVMV
4. Name a pink fruited variety
5. Write in detail about the breeding methods for crop improvement in okra.
References

207
L-29
Moringa
(Moringa oleifera Lam.)
(Hindi : Seeng, Sahgan)
Moringaceae
2n = 22
Moringa/Drumstick is grown for its nutrient rich tender, pods, leaves and flowers
which are used for culinary preparations. Fruits are rich in vitamin C (120 mg/100g),
carotene (110 mg), phosphorus (110 mg) and minerals like magnesium (28 mg), potassium
(259 mg), sulphur (137 mg) and chlorine (423 mg). The crop is grown in most homesteads
in India. Tender leaves and flowers are comparable to that of colocasia in vitamins and
minerals and have great role for combating malnutrition of urban and rural masses. Certain
morigna types principally grown for its foliage are reported from West Indies. Drumstick
roots are good substitute for horse radish. Root, bark and seed have many industrial uses
also.
Originated in South West India, drumstick became a popular vegetable in South
Indian states. The crop is widely distributed in India, Sri Lanka, Pakistan, Singapore,
Malaysia, Cuba, Jamaica and Egypt.
Botany
Drumstick is a small or medium sized perennial tree of about 10 m height with fragile
and corky stem. The leaves are usually tri-pinnate with elliptic leaflets. Pods are pendulous
and length ranges from 20 cm to 100 cm. Seeds are trigonous with wings on angles.
Flowers are produced on current season growth on large and erect panicles or monocladial
208
cyme. Flowers were yellowish creamy white and sweet smelling. Individual flowers are
bisexual, zygomorphic and pedicellate.
Calyx and corolla consist of five sepals and petals. Androecium also has five stamens
alternating with five stamindodes. Gynoecium has a superior, one celled and three carpelled
ovary containing many ovules on parietal placentation. Stigma is truncate.
Flowering in drumstick varies from place to place and is greatly influenced by rain,
temperature, humidity, wind, soil temperature, soil moisture
etc. Under South Indian condition, one or two distinct peak
periods of flowering noticed. Peak period of flowering in
central parts of Kerala is December-January while in southern
part it is February-March and July-August with maximum
flowering in February-March. Under Coimbatore and
Bangalore conditions, flowering seasons are March-May and
July-September respectively. Anthesis continues throughout
the day. Two anthesis peaks i.e., 2.00 p.m and 4.00 a.m. are
noticed at Thiruvannthapuram. In most parts of Tamil Nadu,
flowering is from 4.30 a.m. to 6.30 p.m.
In southern part of Kerala, stigma becomes receptive one day prior to flower opening
and continues with maximum receptivity on the day of opening and a sudden decline
thereafter.
Two common species

209
• Moringa oleifera
• Moringa concanensis
M. oleifera
• Leaves usually tripinnate, leaflets 12-18 mm long,
• Petioles yellow (or) white without red streaks and the tree is medium sized.
M. concanensis
• Bipinnate leaves, leaflets 15-30 mm long, petals with red streaks (or) reddish at base and the
tree is large.
Breeding objectives
• Breeding varieties with dwarf stature.
• Varieties suitable for leaf purpose
• Development of high yielding types
• Breeding varieties with more seed and oil content
• Development of types resistant to pest and diseases
Breeding Methods
• Mass selection
210
• Selection of types starts with open pollination.
• Select one line with the highest potential and test in various conditions and various sites.
• Then, go for control pollination.
• PKM-1 annual moringa was released through this method.
• Hybridization
• Annual moringa PKM- 2 is a hybrid derivative from the cross between MP31 and MP28.
• PKM 2exhibits 48% increase in yield over PKM1.
• Mutation breeding
• Very little work has been carried out in annual moringa through mutation breeding.
Varieties / cultivars
A number of local cultivars are known by the place of their cultivation. Details of local
cultivars are given:
 Jaffna moringa - a perennial type which bares 60-90 cm long pods with soft flesh
and good taste.
 Chavakacheri muringa - a perennial type producing 90-120 cm long pods.
 Chemmuringa- This perennial type flowers throughout year and bears red tipped
fruits.
 Yazhpanam muringa – same as Jaffna type
 Pal muringai – Pods having thicker pulp and better taste

211
 Puna muringa– Thinner fruits.
 Kodikal muringa– produces short pods of 15-20 cm long and is sued as support for
betel vine plants. Propagated by seeds.
There are only a few named varieties and the details are given below:
KM-1 (Kudumianmalai 1)– Bushy variety propagated through seeds. Plants come to bear 6
months after planting and can be rationed for 2-3 years. Productivity 400-500 fruits / year.
Developed at Anna Pannai, Kudumianmalai of Pudukottai.
PKM – 1 – This ―seed moringa‖, propagated through seeds is developed at Horticultural
Research Station, TNAU, Periyakulam. Plants grow to a height of 4-6 m and come to flower
in 90-100 days after planting. The first harvest starts 160-170 days after planting and on an
average each tree bears 200-225 fruits / year. Pods are 65-70 cm long with 6.3 cm girth and
150 g weight. Fruits are green coloured and highly pulpy.
PKM 2– This ―seed moringa‖, propagated through seeds, is also developed at Horticultural
Research Station, TNAU, Periyakulam. Pods are extra long (125-130 cm), pulpy and suitable
for homestreads.
212
Dhanraj– This is also an annual drumstick propgated through seeds and is evolved at KRC
College of agriculture, UAS, Arabhavi, Karnataka.
Crop improvement programmes in the Department of Olericulture, Kerala Agricultural
University, Vellanikkara resulted in the development of three promising perennial drumstick
clones viz., MO 70, MO 95 and MO 44 and one annual seed drumstick, AD 4.
Questions
1. Name some moringa varieties developed from HCRI, Periyakulam
2. Name some seed moringa varieties
3. Name some local cultivars in moringa
4. What are all the breeding methods for moringa
5. Write in detail about the breeding achievements in moringa
References

213
L-30
Peas
INTRODUCTION
Scientific name : Pisum sativum
Common names : Matar (Hindi, Nepali) Pea; split pea, garden pea.
Family : Fabaceae
Chromosome number:2n=14
ORIGIN AND DISTRIBUTION
 The Mediterranean region, western and central Asia and Ethiopia have been
indicated as centers of origin.
 Recently the Food and Agriculture Organization (FAO) designated Ethiopia
and western Asia as centers of diversity, with secondary centers in southern
Asia and the Mediterranean region.
 Archaeological evidence of the use of peas dating from 8000 BC has been
found in the Fertile Crescent.
 The first cultivation of peas appears to have been in western Asia, from where
it spread to Europe, China and India.
Species:
 Ancestor of Pisum sativum –Pisum elatius
 Garden peas - P. sativum ssp. hortense Asch. & Graebn.,
 Field peas - P. sativum ssp. arvense (L.) Poir.,
 Edible podded peas - P. sativum ssp. Macrocarpon
 Early dwarf pea -P. sativum var humile
Types of peas
Garden peas :
 Garden peas have rounded pods that are usually slightly curved in shape
with a smooth texture and vibrant green colour.
 Inside of them are green, rounded pea seeds that are sweet and starchy in
taste.
Snow pea and Snap pea:
 Snow peas are flatter than garden peas and are not fully opaque. Snap peas, a
cross between the garden and snow pea, have plump pods with a crisp, snappy
texture.
214
 The pods of both snow peas and snap peas are edible, and both feature a
slightly sweeter and cooler taste than the garden pea.
Snow Peas Snap Peas
BOTANY
Habit:
Pea is an annual herbaceous plant or leguminous crop.
Roots:
Plants have a taproot system with nodules on the surface.
Stem:
Stems are hollow, slender, succulent and ridged.
Leaves:
It bears pinnately compound leaves with three pairs of leaflets and the terminal one is
modified into a branched tendril.
At the base of the petiole, a large pair of stipules or bracts is found, and they cover the
petioles in such a way that the leaves appear to be sessile.
Seeds:
Seeds are globose or angled, smooth or wrinkled, whitish, grey, green, or brownish.
Floral Biology
 Exclusively self pollinated crop.

 Favoured by cleistogamy.
 Stigma is receptive several days prior to anthesis – protogynous in nature.
 Pollen remains viable from the time of dehiscence of anthers until several days
thereafter.
 Bud pollination is effective.
215
Peas flower
BREEDING OBJECTIVES
 Early maturity
 Pod characteristics
 Dark green pods
 Bold seed size
 Shelling percentage
 Pod yield
 Suitability for processing
 Resistance to disease
 Resistance to insect
216
 Resistance to abiotic stress

BREEDING METHODS
 The breeding procedure adopted for pea improvement include,
 Introduction : Bonneville (USA)
 Individual plant selection( P. 88)

 Pedigree method
 Single seed descent method of selection
 Bulk method of selection
 Recurrent selection
 Hybridization( Jawahar Matar 1, 2, 3 and 4)
 Backcrossing
 Line breeding
 Resistance breeding: Powdery mildew: JP. 83, JP.71, JP.4, PRS 4
Improvement of pea by breeding has been undertaken at PAU(Ludhiyana),

HAU(Hisar), GBPUAT(Pantnagar), JNKV(Jabalpur), CSAU(Kanpur),
Dr.YSPUHF(Solan), IARI(Delhi), PDVR(Varansi), NDUAT(Faizabad) and
VL(Almora).
Inheritance of qualitative characters:
Gene Character
Plant height
cry Influences internode length and plant
height along with
la and le
la Internode length and plant height along
with cry and le
le Internode length and plant height along
with cry and la
Colour
a Absence of anthocyanin :
dominant allele for anthocyanin
production in plant, flower and
seed
Ch-l Plant light yellowish green
d Green leaf axil : D dependent on A for
manifestation of colour
217
pa Dark green immature seed and foliage

vm Effect to similar to pa
Inflorescence , Number of flowers
fn With fna determines number of
flower on the inflorescence
:greatly influenced by
environment
fna With fn determines number of flowers on
the
inflorescence, greatly influenced by
environment
Branching
fr With fru determines number of basal
branches
fru With fr determines number of basal
branches
ram Increases number of branches
Leaves and stipules
af Leaflets converted into tendrils
lat Double leaflet and stipule area
tac Tendrils present on acacia leaves
tl Leaves with extra leaflets and no tendrils
Pods
it Increases pod with 25 per cent
Bt Apex of pod blunt
Com Affects curvature of pods
n Pod Wall thick
Dpo Pods tough and leathery : readily dehisce
at maturity
P Reduces or eliminates
sclerenchymatous membrane on
inner pod walls
V Same as p
gp Young pod yellow
pu With A Pur pod colour purple
pur With A Pu pod colour purple
218
Varieties
Pea cultivars grown in different parts of the world exhibit wide variation in
height of stem, branching, pod size, seeds per pod, shelling percentage, smoothness of
seeds (smooth / wrinkled) etc. The cultivars / varieties are grouped based on various
characters as given below:
Based on maturity period
 Early types – green pods will be ready for harvest by 65 days after sowing.
 Mid season types – pods will be ready for harvest by 85-90 days after sowing.
 Late main season types – pods will be ready for harvest by 110 days after sowing
 Based on height of plant
 Bush or dwarf types
 Medium tall
 Tall
Usually dwarf types are early and mid season types are medium tall. Late types are
tall and require support.
Varietal Achievement
Cultivar Parentage Salient features Institution
Early Group
Arkel Introduction Early season variety introduced from England IARI, New
from England Dwarf plants bearing double pods at lower nodes Delhi
and single at upper nodes. Pods 8.8 cm long and
sickle shaped. Suitable for fresh market and
dehydration. Susceptible to collar rot at high
temperature. Yield 7.5 t/ha in 50-55 days.
Pusa Pragati - Yield 7 t/ha in 60-65 days. IARI, New

Delhi
Jawahar T19 x Early Early season variety developed through selection JNKVV,
Matar3 from cross between T 19 x Early Badger. First Jabalpur
badger
picking in 50 DAS, Pods 7 cm long, light green
and round oval / ovules.
T19 x Little
JM4 Yield 8 t/ha in 55-60 days. JNKVV,
Marvel Jabalpur
219
Pant Matar2 Early Badger x Yield 6 t/ha in 55-60 days. GBPUAT,

IP3 Pant nagar
Hisar Harit Selection from Pods large sickle shaped and single or double. HAU, Hisar
cross between Yield 9 t/ha.
Bonneville x P
23.
Kashi Mukti Pedigree powdery mildew resistant variety developed IIVR,

selection from through Yield 110-120 q/ha Varanasi
the cross No. 7
x PM-5.
Kashi Pedigree Plant height is 47-51 cm, first flower 32 IIVR,

Nandini selection from DAS, bears 7-8 pods per plant. Pods are 8-9 Varanasi
the cross P cm long, attractive, length 8-9 cm, well
1542 x VT-2- filled with 8-9 seeds, shelling percentage
1. 47-48, yield 110-120 q/ha. It is tolerant to
leaf mine and pod borer
Matar _ Early season dwarf variety. Tolerant to high PAU,

Ageta 6 temperature. Yield 6 t/ha with 44.67% shelling Ludhiana
percentage. Seeds smooth and green.
Mid season and late group
Bonneville Introduction Mid season variety introduced from USA. Medium IARI, New
from USA tall plants bearing double pods. Pods more than 9 Delhi
cm long. Yield 8.5 t/ha. Seeds green and wrinkled.
Lincoln Introduction Early season variety introduced from France. IARI, New
from USA Medium tall plants bearing double pods of 8-9 cm Delhi
length and sickle shaped. Mature seeds wrinkled.
First picking 85-90 days after sowing (DAS). Yield
68-10 t/ha.
Jawahar T19x Greater Mid season dwarf variety with big, attractive green, JNKVV,
Matar 1 8-9 cm long pods containing 8-10 sweet green Jabalpur
Progress
ovules
Pods dark green, big, curved with 8-10 sweet JNKVV,
Jawahar Russian 2 x
ovules, wrinkle seeded, susceptible to powdery Jabalpur
Matar 2 Greater
mildew.
Progress
Pant - Medium maturity, ready for harvest by 70-80 DAS. GBPUAT,

Uphar(IP3) Flowers white, Pods round. Seeds wrinkled,
Susceptible to powdery mildew. Resistant to stem Pantnagar
fly. Yield 10 t/ha.
Punjab 88(P Pusa 2x Early season variety. Pods dark green, long (8-10 PAU,
cm) and slightly curved. Days to first harvest –
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88) Morrasis 55 100. Yield 15 t/ha with 47% shelling percentage. Ludhiana
VL Matar 3 Old Sugar x Plants determinate. White flowers, straight and VL, Almora
Early double podded. Length – 6.8 cm. First picking is
Wrinkled 100 DAS. Yield 10 t/ha.
Dwarf 2-2-
Mithi Phali - Yield 11-12 t/ha in 90 days. PAU,

Ludhiana
JP 19 - Yield 10-11 t/ha in 90 days. JNKVV,

Jabalpur
Ooty-1 Dwarf variety having a yield potential of 11.9 t/ha in TNAU,

90 days. Resistant to white fly. Coimbatore
Breeding for Disease Resistance
Disease Resistance Source Institution
Powdery mildew Jawahar Pea 83 JNKVV, Jabalpur

(Erysiphe polygoni)
JP4(JM 6), PRS4, FC 1
Fusarium wilt (Fusarium Kalanagini Local cultivar

oxysporum f. sp. JNKVV, Jabalpur
JP179
IARI, New Delhi
Pisi) PushaVipasha
Rust JP.Batri Brown3 JNKVV, Jabalpur

(Uromyces fabae) Jp.Batri brown4
Aschochayta blight Kinnauri Local cultivar

(Aschochayta pisi)
Bean yellow mosaic Bonneville IARI, New Delhi

virus
Breeding for resistance to insect pests
Pest Resistance Variety Institution
Leaf miner (Phaytomyza LMR-4,LMR-10,LMR- HAU, Hisar

articormis) 20
Bruchus JP 9 ,JP 179, JP JP Batri JNKVV, Jabalpur

(Collosobruchus Brown 3, JP Batri
221
chinensis) Brown 4
Multiple disease and pest resistance
Pest/Diseases Resistance source Institution
Highly resistance to JP 179 JNKVV, Jabalpur

powdery mildew, tolerant
rust ,resistance to
Fusarium
wilt, Bruchus, leaf miner
Resistance to powdery JP 9 JNKVV, Jabalpur

mildew, bruches
Resistance to powdery JP 501 JNKVV, Jabalpur

mildew, wilt, bruches
Resistance to rust and Arka Karthik, Arka IIHR, Bangalore

powdery mildew Sampoorna
Rondo – High Yielding Variety
Kashi Nandhini Kashi Mukti
MUTATION BREEDING
Mutants:
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Afila:
Leaflets are converted into tendrils.
Acacia:
 Tendrils are converted into leaflets.
 The induced mutations developed are the early flowering
46 C and JP 829 (Flowering from 4th to 6th node),
Fascinated mutants, R701, R710, JP 625,JP 67,251 A, 997,999 and others.
Breeding for processing qualities
 Dehydration, canning and freezing are the most common processing method of peas.
 Large sized wrinkled and dark green peas like Arkel are suitable for dehydration, for
canning, both round and wrinkled seeded varieties like T19 and Bonneville can be
used and for freezing wrinkled seeds.
Biotechnology:
 There are several reports on micropropogation of peas by tissue culture using apical
meristems of seedling, immature and mature leaves, axillary buds primary scales and
cotyledons.
 Plants could be regenerated by protoplasm through somatic embryogenesis.
 For genetic transformation Agrobectarium tumefenciens and micro projectile had been
used.
 Production of transgenic plants in peas has also been reported.
 Recently detailed genetic maps have been assembled in eight genera of the fabaceae,
including Phaseolus, Pisum,Vicia and Vigna.
 There is a common gene order across at least 40 per cent of the lentil and pea
genomes and conservation with many chromosomal regions in Vicia.
 Studies are in progression comparative genomics and legumes.
 Detailed genetic map has been developed for pea.
 Genes of interest have been located and closely linked markers have been identified.
 Marker assisted selection procedure using RFLP, RAPD and isozymes have been
established for pea venation mosaic virus, pea seed borne mosaic virus, and powdery
mildew resistance.
Questions
1. What are all the relative species of Peas?
2. Write about the floral biology of peas
3. Write in detail about the breeding objectives for peas
4. Write in detail about the resistant breeding in peas
5. Name a variety developed from TNAU
References
223
L-31 FRENCH BEANS

Phaseolus vulgaris L.
Fabaceae
2n = 22
Common Name: Snap bean, String bean, Kidney bean, Haricot bean, Fresh bean
Hindi: Vilaiti sem
The French bean (syn. Kidney bean, haricot bean, snap bean, navy bean) is one of the most
important leguminous vegetables. It is grown for the tender vegetable, shelled green beans and dry
beans (rajmah) as a pulse.
Origin : South mexico and Central America
There are four cultivated species in the new world:
Phaseolus vulgaris : The common, haricot, navy, French or snap bean.
P.coccineus : The runner or scarlet runner bean.
P.lunatus :The Lima (large-seeded), sieva (small-seeded), butter

or Madagascar bean.
P.acutifolius var. latifolius : The tepary bean
All the species are diploid with 2n = 2x = 22. P.coccineus ( Scarlet runner bean) is generally
cross-pollinated and the other three species are self-pollinating with only a small amount of cross-
pollination.
Ancestor of French bean: Phaseolus aborigineus
•Potato bean - Pachyrrhizus tuberosus .Tuber is used as vegetables
 P.aborigineus in North West Argentina as the wild progetor of French beans
Based on growth habit it can be classified into
pole type, semi pole type and bushy type,
1.Indeterminate - 17-35 internodes
2.Indeterminate - Semi climber 14-30 internodes

224
3.Indeterminate - Bush 13-12
4.Determinare climber - 12-15
5.Determinate bush
Classification of cultivars
The French bean cultivars are classified as follows.
Dwarf or bush types
Green pods Wax pods
String Stringless String Stringless
Masterpiece Processor Nil Cherokee Wax

Green Ruler Golden Ruler
(Honma and Bedford, 1976) (Honma and Cash, 1979)
225
Climbing (pole) types
Green pods Wax pods
String Stringless String Stringless
Tender and True Blue Lake Nil Kentucky Wonder

Onatra Wax
(Stolk, 1980)
There is a large number of French bean cultivars.
1. Snap beans - for vegetable pods.
2. Green-shell beans - used in the green-shelled condition.
3. Dry-shell beans - used in the dry state (field beans).
Each group is again divided into climbing (pole) and dwarf (bush) types.
Some of the green-shell bean cultivars are Low‘s Champion, French Horticultural, Dwarf
Horticultural, Brilliant, Flash and Green Lime Light which are bush type cultivars. Pole type
cultivars are London Horticultural and Red Cranberry.
Some of the important snap beans cultivars are as follows.
There are three types of bush beans, namely, flat, oval and round types.
226
The flat types are Bountiful, Plentiful, Green Ruler, Golden Ruler and Romano.
The oval types include Pusa Parvati, Contender, (disease-tolerant, green and prolific bearer),
Spartan Arrow, Premier, Tendergreen, King Green, etc.
The pole types include Blue Lake, Kentucky Wonder and Phenomenal Long Podded. Most of
the local types native to North-Eastern Region of India are pole types.
Tender crop and Cascade are popular processing cultivars. However, the latest
concept in the U.K. is to go in for cultivars with short pods so that they can be processed
whole.
FLORAL BIOLOGY
• Flower: ( Inflorescence) Racemose
• Anthesis: 11:30am-12pm
• Anther dehiscence: 88%
• Stigma receptivity: 10 hrs after anthesis
• Fruit is usualIy a Legume or pod
Crop improvement
The French bean improvement is being carried out in some Research Institutes and
Agricultural Universities in India.
This has led to the identification/evolution of cultivars like VL Boni (Almora); UPF-191,
UPF-204, etc., (Pantnagar); SVM (an interspecific cross) from Solan; Sel.2, Sel.5 and Sel.9
(Bangalore) and Pusa Parvati from Indian Agricultural Research Institute, New Delhi, India.
All the research institutes excepting Indian Council of Agricultural Research Complex,
Shillong are involved in evolving bush bean lines.
The work in North-Eastern region of India is towards evolving pole bean cultivars. Because
of high rainfall, farmers prefer pole type as the fruiting height is much above the ground level thus
preventing the pods from being infected with pathogens from soil.
Besides, there are many local pole types being grown which can be improved for specific
traits like yield and quality.
Breeding objectives
227
1. High yield
2. Quality for fresh market and sloughing free cultivars for canning industry
3. Short pods for whole pod processing.
4. Resistance to pests and diseases.
5. Exploitation of hybrid vigour by induction of male sterility
P.vulgaris Phaseolus coccineus

228
White Tepary Beans
Botany
French bean has tap root system with poor nodule formation. Leaves are trifoliate.
Though a self-pollinated crop, French bean offers wide variability for plant growth (bushy /
climbing), colour of pod (green / waxy coloured), cross section of pod (flat / oval / round),
pliability (stringed / string less) etc.
229
METHODS OF BREEDING
1.Introduction: Contender, Kentucky wonder
2. Pure line selection : Arka komal, (sel -9), APLS from IIHR-60 collection IIHR-220,PLS
from hungary collection. Arka Bold + rust and bacterial blight.
3.Pedigree method : IIHR-909 pod selection from Blue crop x Contender (Arka Suvidha)
Mutation breeding:
Pusa Parvati derived from a mutant waxy type EC1906, X-ray seeds were exposed.
 Early bushy
 Resistance to anthracnose.
Varieties
There are specific varieties for snap bean purpose, dry bean purpose and for processing.
Processing varieties are very popular in the USA. A brief description of improved varieties is
given below:
Developing Variety Breeding Special features

institution Method
IIHR, Arka Komal* Introduced Bush type.Pods straight, flat, and green with large
Bangalore. bushy brown seeds. Good transport and keeping quality.
variety from Yield 19 t/ha and 3 t/ha seed in 65-70 days.
Afghanistan.
Arka Plants bushy and photosensitive. Pods straight and
Suvidha* oval, light green, stringless and fleshy. Yield 19
(IIHR 909) t/ha in 70 days.
IARI Contender Plants bushy with pink flowers. Pods green, round
Regional long and stringless. Tolerant to mosaic and
Station, powdery mildew. Yield 20 t/ha.
Katrain
Pusa Parvati Developed Plants bushy with pink flowers. Pod green, round
through long. Resistant to mosaic and powdery mildew.
230
irradiation Yield 22-25 t/ha.

followed by
selection
from wax
podded
variety EC
1906.
Pusa Pole variety with medium sized (14 cm long)
Himalatha round, meaty, stringless pods with an average
yield of 26 t/ha.
VPKAS, VL Boni 1* Dwarf variety with white flowers. Pods round,
Almora light green, stringless and fleshy. First harvest 45-
60 DAS. Yield 10-11 t/ha.
Tamil Nadu Ooty-1 Moderately resistant to leaf spot, anthracnose and
Agricultural pod borer. Yield 10-11 t/ha.
University
TKD1 A pole type suitable for growing in hills. Pods
long, flat with low fibre. Yield 5-6 t/ha in 90-100
days.
KKL 1 A pole type suitable for growing in hills. Pods
Moringa bean long (28 cm) with low fibre. Seeds white and flat.
Yield 7 t of pods and 3 t. of grains/ha.
YCD1 Selection Bushy dual purpose variety suitable
from the for kharif season. Pods slightly flat, 15 cm long.
accession PV Seeds dark purple. Tolerant to root rot, rust,
24. yellow mosaic and anthracnose. Yield 9.75 t. of
pods or 6.3 of grains / ha in 105 days.
NDAU&T, NDVP 8* Mid season variety with 10 t/ha
Faizabad,
UP.
NDVP 10* Mid season variety with 10 t/ha.
CBPUA&T, Pant Plants bushy and dwarf with concentrated fruiting.
Pantnagar Anupama* Moderately resistant to bean mosaic and angular
(UPF 191) leaf spot. Yield 9 t/ha.
MPKV, Phule Pods 9-10 cm long, flat, light green. Tolerant to
Rahuri Surekha anthracnose, yellow mosaic and wilt disease.
Yield 15 t/ha.
* Varieties released / identified by AICRP (Vegetables)
231
In addition to the above improved varieties, cultivars like Kentucky Wonder (pole
type with long, flat and stringless pods), Premier, Giant Stringless, Bountiful etc. are also
very popular among farmers.
Contender Kentucky Wonder
PUSA PARVATI
DOLICHOS BEAN
INTRODUCTION
 Dolichos lablab (L.syn. Lablab purpureus)
 Family : Leguminoaceae
 Chromosome no. : 2n = 20, 22, 24
 Origin : Asian
 Spread throughout the tropical and temperate region of Asia, Africa and America.
 Ancestral species : Dolichos purpureus.
 It is leguminous pod vegetable.
GENETICS
 Number of pods per plant, number of seeds per pod and 00 seed weight are highly
heritable and highly correlated with yield.
 Pigmentation of vegetation and flower: monogenic where purple dominant to white.
 Pod colour : Monogenic where green dominant to light green.

232
 Pod character : Flat pod dominant to swollen pod. Fp/Fp
 Seed colour : Monogenic where chocolate (dark brown) is dominant over brown.
MORPHOLOGY
FLOWER COLOUR
POD COLOUR
POLE TYPE
233
BUSH TYPE
SEED COLOUR AND SHAPE
BREEDING OBJECTIVES
 Bushy habit
 High yielder eg Arka Prasidhi 37t/ ha @ 120 d
 Early yielder
 Photo insensitive eg. Konkon Bushan
 High sugar to polysaccharide ratio

234
 Less fiber (string less)
 Fleshy pod pericarp
BREEDING METHODS
 Highly self pollinated crop.

 Pure line selection and back cross:
 Arka Jay (sel-1): Developed from back cross and pure line selection from cross
Hebbal Avare * IIHR 93. Photo insensitive and tolerant to heat and drought.
 Arka Vijay (sel-2): Developed from back cross and pure line selection from cross
Hebbal Avare * IIHR93. Photo intensive and tolerant to heat and drought.
 Bulk population method: Bred DL 2539 and released as CO1 Mochai which is early,
high yielder and drought tolerant
 Pedigree method:
CO 9 (bush type) * Yanaikathu (pandal type)
F6 Colt 22
Named as CO 11 Avarai
 Heterosis breeding
 Hand pollination and emasculation
 Use of protruded stigma
 Gametocides use eg. FW 450
 CO5*DL3196: High yield and better pod quality
 6023 A * 6010 : High ascorbic acid content.
MUTATION BREEDING
 DL 244 (garden bean)* DL 3196 (field bean)

235
CO 6
Seeds were gamma irradiated
In M2 isolated Mutant 3 which is high yielder than CO9 and released as CO10 Avarai
ACHIEVEMENTS
 Pusa Early Prolific : IARI
 Rewa : High protein content (25.00%)
 CO 1: Selection from cross between var. typicus and var. lignosus. It is early and high
protein and fat content.
 CO2 (DL 250)
 CO 3 (DL 269)
 CO 4 (DL 453)
 CO 5 (DL 692)
VARIETIES OF INDIAN BEAN
Pole types Bush types
Pusa Early Prolific, Pusa Sem-2, Arka Jay, Hebbal Avare-3 * IHR99
Pusa Sem-3
Arka Sambhram Arka Vijay, Hebbal Avare-3 * Pusa Early
Prolific
Arka Soumya, Avare-3 * Web-1 Konkan Bhusan (DPLD-1): Hebbal
Arka Komal, Arka bold, Arka Amogh CO-8: CO-5*DL-3169
Kashi Harittima
Rajni, KDB-403,KDB-405
Dasarawal , deepaliwal Mutant variety
JDL-79,JDL-53 CO9- spontaneous mutant MS-98678

236
CO1-selection from HD18 of Hiser CO10: X ray induced mutant of CO6
CO2-selection from Chinna avarai CO11: CO9 x white yanai kathu
CO3-selection from yanaikathu avarai CO12: CO9 x CO4
CO4-selection from sivappu avarai
CO5- selection from kozhikkal avarai
LIMA BEAN
(Syn: Double Bean) (Phaseolus lunatus L.) (2n = 22)(Hindi : Lobia)
Dixie Speckled Butter Peas - Phaseolus lunatus
Christmas Lima Beans - Phaseolus lunatus
Hopi Yellow Lima Beans - "Sikyahatiko" - Phaseolus lunatus
Lime bean is a cool season vegetable requiring dry and cool climate with an average rainfall
of 50-62.5 cm. Compared to other legumes, it is a long duration crop and is retained in field
for 9 months. Lima bean is an important crop in Maharashtra.
237
BROAD BEAN
(Vicia faba L.) (2n = 12, 14)
(Hindi: Bakla)
Broad bean, also known as faba bean or horse bean, is the only bean, sown in autumn and is
grown as a winter crop in high elevations. It is a hardy plant and withstands low temperature
as low as 4oC. The crop is widely cultivated in Latin America and is grown in India in a
limited scale in northern states having low temperature. It tolerates salinity to a certain
extent. Broad bean issued as tender bean, green shelled bean, and dry bean and as cattle feed.
Plants are more or less vine like and grow erect without branching to a height of 60-125 cm.
Stem is square in cross section and flowers are pollinated by insects. Pods are borne in
upright clusters of 5 or more in axils of leaves. Green pods grow parallel to stem. It is about
15 cm long and 2 cm wide with slightly round in cross section with 5 or more beans.
Varieties
Masterpiece White Long Pod, Masterpiece Green Long Pod, Imperial White Windsor and
238
Imperial Green Windsor are some of the introduced varieties. Jawahar Selection 73-31 is an
improved selection from Madhya Pradesh. A few selections made at Bihar are BR-1 (black
seeded) and BR-2 (yellow seeded). M/s.Suttan Seeds developed a dwarf type ‗Suttan White
Seeded‘.
Anasazi Beans(Phaseolus vulgaris)
Bolita Beans (Phaseolus vulgaris)
Purple Beans (Phaseolus vulgaris)
Hopi Black Pinto Beans (Phaseolus vulgaris)
Questions
239
1. Write in detail about the crop imprvement in French bean

2. Write in detail about the crop imprvement in Broad bean
3. Write in detail about the crop imprvement in Dolichos bean
4. Write in detail about the crop imprvement in Lima Bean
5. Write in detail about the classification of french bean cultivars
References
1.Kumar, N. 2006. Breeding of Horticultural crops: Principles and Pracitices. New India
Publishing Agency, Pitam Pura, NewDelhi.
2.Kalloo, G. and B.O.Bergh.1993. Genetic improvement of vegetable crops. Elsevier Ltd
3. Peter, K.V. and T. Pradeepkumar. 2008. Genetics and Breeding of Vegetables, Revised, ICAR,
New Delhi.
L-32
Amaranthus
Amaranthus sp. (2n = 32, 34, 64) - (Hindi: Chaulai)
Amaranthaceae
Amaranthus is the most common leaf vegetable grown in Kerala and Tamil Nadu.
Leaves and succulent stem are good sources of iron (38.5 mg/100g), calcium (350-400
mg/100g), vitamin A and vitamin C. Absorption of calcium from amaranth is however poor.
The iron availability is only about 15.2 – 53.6% of total iron. High oxalate content (1-2 %)
and nitrate (1.8-8.8 g/ kg dry matter) levels are reported from leaves of various species. Short
duration, quick response to manures and fertilizers, high yield, easiness in cultivation and
availability of diverse types suited to specific agro-climatic situations make it a favourite crop
of farmers to fit in any cropping systems. Both leaf and grain types play a vital role to
combat malnutrition of poor people.
Centres of diversity for amaranth are Central and South America, India, South East Asia with
secondary centres of diversity in West and East Africa. Leaf amaranth is a native of
240
India.Taxonomists recognize two sections in Amaranthus, viz., Amaranthus and
Blitopsis, Section Amaranthus, includes important grain types where the inflorescence is
terminal. Section Blitopsis includes leaf types and flowers are borne in clusters in leaf axils.
Majority of leaf cultivars grown in India belong to Amaranthus tricolor. Major species found
in India are:
A. tricolor (Syn) A. gangeticus,
A. mangostanus, A. polygonoides cultivated - leaf type
A dubius cultivated -leaf type
A. blitum (Syn: A. lividus) cultivated - leaf type
A. tristis wild - leaf type
A. viridis wild - leaf type
A. spinosus cultivated - grain type
A. cruentus cultivated - grain type
A. caudatus cultivated - grain type
A. viridis
241
A. tristis A. blitum A. dubius
A. spinosus A. cruentus
242
A. caudatus
Botany
Amaranth is an annual herb with erect growth and scarce to profuse branching habit.
Stem is succulent and green or purple or mixed shades of these two. Leaf is simple, alternate,
with obviate to lanceolate shape. Leaf colour is green or red or with different shades of
above. Flowers are borne terminally and in axils of leaves in clusters. Basic unit of
inflorescence is called as glomerule. Flowers are small, unisexual and monoecious. Most of
cultivated types are monoecious. Proportion of male and female flowers varies in an
inflorescence. Each glomerule consists of a staminate flower and a number of pistillate
flowers. The extent of cross pollination is governed by proportion of male and female
flowers in an inflorescence and position of inflorescence in plant. Percentage of male flowers
in a glomerule is 0.5 in grain types and 10.25 in leaf types. According to Pal and Khoshoo
(1978),leaf amaranths are predominantly self pollinated due to presence of a large number of
male flowers per glomerule, terminal inflorescence and development of axillary glomerules.
Grain types favour cross pollination.

243
Stigma of pistillate flower is receptive several days prior to opening of staminate
flowers in an inflorescence. Wind help in transfer of pollen grains from male flowers of a
glomerule / inflorescence / plant to another glomerule / inflorescence / plant. But grain
species with colorful inflorescence are occasionally visited by bees (Khoshoo and Pal,
1970).Chromosome number varies with species in amaranth. The diploid species have 2n=32
or 34. A tricolor is with 2n=34 white A. cruentus and A. tristis have 2n=32. The tetraploid
species, A dubius has 2n=64.
Breeding objectives
Breeding for low anti-nutrient factors – low oxalate and low nitrate lines
Breeding methods
Mass selection – common method
CO 2 – from local – A. tricolor

244
CO 1 – A. dubius - Leafy types - green

CO 3 – A. tricolor var. tristis
CO 4 – A. hypochondriacus – Grain type
• To generate variability
• To transfer desirable characters to cultivated spp.
Polyploid breeding
• A. dubius – natural polyploid – allopolyploidy
- 2n = 16 + 16
• A. blitum – Colchicine induced tetraploids – larger

and numerous leaves – longer vegetative cycle
• A. dubius - Seeds and seedlings – treated with
colchicine
- Polyploidy – reduction of plant height,

leaf size and inflorescence length.
- Pollen and seed sterility
Hybridization and selection
Monoecious
A. spinosus – upper part of panicle – male flowers -

topping for emasculation. Lower part with female
flowers are bagged
A. tricolor, A. lividus, A. dubius – Emasculation tedius
• Intermixed nature of male & female flowers within same glomerule

• Emasculation incomplete – due to subsequent opening of male flowers
Anthesis – 8.30 – 9.30 am
• Pollination is repeated for 3 consecutive days – all female flowers are not
receptive on the same day
245
Crossing – enclosing a part of ripe inflorescence of male parent in the bag with
emasculated inflorescence
Varieties
Cultivated leaf amaranth varieties and cultivars differ in size, shape and colour of leaves and
stem, position of inflorescence etc. and belong to different species. A brief description of
improved varieties developed by different Research Institutes is given below:
1) Tamil Nadu Agricultural University, Coimbatore-3
This tetraploid variety was developed by selection from ("local
CO. l germplasm. Stem and leaves are dark green; leaf-stem ratio is
(A. dubius) 2.0; inflorescence terminal and axillary; lacks initial vigour but
makes rapid growth after 30 days; suitable for late harvest;
resistant to Rhizoctonia leaf blight; green yield 8.0 t/ha; seed
yield 1.5 t/ha.
CO.2 Stem and leaves green, leaves lanceolate and slightly elongate,
(A. tricolor) leaf-stem ratio 1.8; suited for early harvest; yield 10.78 t/ha.
CO.3 This is specifically suited for clipping of tender greens and is
(A. tristis) locally known as ‗Araikeera' in Tamil. Leaves are small and
green; stem is slender and tender. First clipping is possible in 20
days after sowing. Nearly 10 clippings can be taken over a
period of 90 days. Due to very high leaf-stem ratio, cooking
quality and taste are excellent. Special care is required in land
preparation for the variety.
CO. 4 This grain type makes rapid vegetative growth within a period
(A.hypochondriacus) of 20-25 days. Plants are dwarf; grain yield 2.0-2.5 t/ha in 80-90
246
days.
CO.5 Leaves double coloured with Green and pink and is free from
(A. tricolor) fibre. It gives a rosette growth in early stages and first harvest is
possible in 25 days; yield 40 t/ha in 55 days.
Sirukeerai (A. polygonoides) is a traditional cultivar in Tamil Nadu, suited for uprooting at
25 days after sowing; leaves are small, ovate with blunt bifurcated tip and have long petiole;
collar region is dark pink and at leaf axil a miniature branch initiates.
2) IARI, New Delhi
Pusa Chotti Chaulai (A. blitum): Plants dwarf with succulent, small and green leaves;
responds well to cutting.
Pusa Badi Chaulai (A. tricolor): Plants tall and stem thick with large green leaves; responds
to cutting.
Pusa Kirti (A. blitum): Green leaved variety with green and thick stem; leaf lamina broad
ovate; ready for harvest in 30-35 days and extends up to 70-85 days; yield 55 t/ha;
specifically suited for summer.

247
Pusa Kiran (A. tricolor): This is developed by natural crossing between A. tricolor and A.
tristis and has more characteristics of A. tricolor. Leaves are glossy green with broad ovate
lamina; leaf-stem ratio is 1.0:4.6; yield 35 t/ha in 70-75 days; suited for kharif season.
Pusa Lal Chaulai (A. tricolor): Upper surface of leaves are deep red and lower surface
purplish red; yield 45-49 t/ha in 4 harvests.
3) IIHR, Bangalore
Arka Suguana (A. tricolor): A multicut variety with broad green leaves. First picking starts
in 24 days after sowing and continue up to 90 days. Moderately resistant to white rust. Yield
17-18 t/ha.
Arka Arunima (A. tricolor):A multicult variety with broad dark purple leaves. First picking
starts in 30 days after sowing and two subsequent cuttings at 10-12 days interval. Yield 27
t/ha.
Questions
248
1. Which is the centre of origin of Amaranthus ?
2. Write down the breeding objectives for amaranthus
3. Write down the breeding methods for amaranthus
4. Write down the relative species of amaranthus
5. Write in detail about the breeding achievements in amaranthus
References
 Kalloo, G. and B.O.Bergh.1993. Genetic improvement of vegetable crops. Elsevier Ltd

 Saubhik , D. 2016. Amaranthus-A Promising Crop of Future Hardcover , Springer
Publications.
Issue of patenting, PPVFR Act L-33
Patents represent a contract between an inventor and society. By granting time-limited
market exclusivity, patents create the potential for inventors to generate high returns on
successful innovations. In exchange, the inventor provides a complete description of the
invention so that others may build on the technology to create improvements or other
breakthrough discoveries. Patent protection of intellectual property is particularly important
to inventors in the biotechnology field because of the relatively high fixed cost of research
and the ease with which discoveries may be copied. By attracting investment capital for
research, patent protection increases the pace of innovation, thus benefiting society.
The Patent Act, 1970 specifies what are not considered inventions under Section 3.
Section 3(b) of the Act earlier recited: ‘What are not inventions – an invention the primary or
intended use of which would be contrary to law or morality or injurious to public health;‘
249
This definition was brief and the words tapered the scope of the section. An amendment was
brought in 2002, to accommodate the TRIPS regulations. The amendment resulted in a new
and more descriptive definition of the section. The section now reads –
‘3(b) an invention the primary or intended use or commercial exploitation of which
could be contrary to public order or morality or which causes serious prejudice to
human, animal or plant life or health or to the environment;’ [Is not invention]
The Amendments:
TRIPS mainly aimed at excluding subject matter relating to medical methods, or those
contrary to public order and morality, and subject matter covering plants and animals, and
essentially biological processes from being patentable subject matter.
The amendments were hence made in 2002 to bring under the Act, the regulations of TRIPS
and to extend the scope of the Act to protect plants, animals and human beings alike. Along
with Section 3(b), Sections 3(h), 3(i), 3(j) and Section 3(p) were amended with a similar point
of view.
According to Section 3(h), methods of agriculture or horticulture are not patented. Further,
Section 3(i) prohibits patenting process used in medicine and treatments of human beings and
animals, both either as part of treatment and for increasing their economic value or the
economic value of their products.
Section 3(j) includes further provisions to prevent patenting of plants and animals as a whole
or in part thereof. The Section further prevents patenting of ‗biological processes for
production or propagation of plants and animals’. However, micro-organisms are patentable
subject matter.
Section 3(p) prohibits patenting of traditional knowledge.
Coming to the point where these sections are considered eco-friendly, it is important to note
that all the above mentioned sections have a direct impact on the protection of bio diversity.
250
The debate on Technology V. Bio diversity will always appear in the front row, and as
always, a check on the use of technology is the only supportive argument.
The Seed Act 1966, the Bio-diversity Act, 2002, The Protection of Plant Variety and
Farmer‘s Rights Act, 2001 are all laws brought forward to support the cause. For example,
the Section 6 of the Biodiversity Act says- ‗Application for intellectual property rights not to
be made without approval of the National Biodiversity Authority‘.
The Laws:
Laws that are in contrary to public order and morality have always faced criticism. Article 19
of the Indian Constitution holds any Act violating or hindering the operation of law an
offence. In other words the main aim of any law is to bring order and to protect the moral and
social values in the society.
The patent laws emphasize the need for novelty, non-obviousness, utility and the presence of
a human intervention, when it comes to biological matter.
Plants and Patents:
Though ―Bio-Diversity‖ is an ‗all-inclusive‘ word, it is more often used as a synonym for
―Plants and Animals‖
Inventions that drastically affect both Plants and Animals are not patentable under the Section
3(b).
This however is not accepted without arguments that for more than one reason, people would
want better technology. The need is as simple as the explanation for livelihood. Man depends
on plants and animals in numerous ways and inventions that help better are always welcome.
Be it the chemical preparation of weedicides and insecticides or the enormous saws that bring
down gigantic trees, man wants it all. For example, US7520118 and EP1583434 are patents
that are granted to machines that cut down plants. Genetically modified crops are also a big
hit in the market.

251
In R.W.Emerson‘s words, a weed is a plant whose virtues have not yet been discovered.
However, the general perceptions of weeds outweigh the good points about them. Where on
one side one may argue about soil stability, a farmer would argue about the reduced crop
quality.
Monsanto, an agricultural company tried to claim protection to its ―Method for producing a
transgeneric plant (with increased stress tolerance)‖ the application was rejected protection
by India‘s Patent Office, and the same was upheld by the Intellectual Property Appellate
Board on the grounds that the invention lacked inventive step.
The Protection of Plant Variety and Farmers Right Act, 2001 (PPVFR Act) is
an Act of the Parliament of India that was enacted to provide for the establishment of an
effective system for protection of plant varieties, the rights of farmers and plant breeders, and
to encourage the development and cultivation of new varieties of plants. This act received the
assent of the President of India on the 30 October 2001.
The PPV&FR Act, 2001 was enacted to grant intellectual property rights to plant breeders,
researchers and farmers who have developed any new or extant plant varieties. The
Intellectual Property Right granted under PPV&FR Act, 2001 is a dual right – one is for the
variety and the other is for the denomination assigned to it by the breeder. The rights granted
under this Act are heritable and assignable and only registration of a plant variety confers the
right. Essentially Derived Varieties (EDV) can also be registered under this Act and it may be
new or extant. Farmers are entitled to save, use, sow, re-sow, exchange or sell their farm
produce including seed of a registered variety in an unbranded manner. Farmers' varieties are
eligible for registration and farmers are totally exempted from payment of any fee in any
proceedings under this Act. The period of protection for field crops is 15 years and for trees
and vines is 18 years and for notified varieties it is 15 years from the date of notification
under section 5 of Seeds Act, 1966. Annual fee has to be paid every year for maintaining the
252
registration and renewal fee has to be paid for the extended period of registration. Farmers
can claim for compensation if the registered variety fails to provide expected performance
under given conditions. The rights granted under this Act are exclusive right to produce, sell,
market, distribute, import and export the variety. Civil and criminal remedies are provided for
enforcement of breeders' rights and provisions relating to benefit sharing and compulsory
licence in case registered variety is not made available to the public at reasonable price are
provided. Compensation is also provided for village or rural communities if any registered
variety has been developed using any variety in whose evolution such village or local
community has contributed significantly. The procedural details and modes of implementing
this Act are provided in PPV&FR Rules, 2003. In the present article, we are discussing some
of the important legal provisions of this Act along with some of the case studies.
The Protection of Plant Varieties and Farmers' Rights Authority also confers Plant
Genome Savior "Farmer Reward" and "Farmer Recognition" to the farmers engaged in
the conservation of genetic resources of landraces and wild relatives of economic plants and
their improvement through selection and preservation and the material so selected and
preserved has been used as donors of gene in varieties registerable under the PPV&FR Act,
2001 (53 of 2001) Upto 10 rewards and 20 recognitions (consisting of a citation, memento
and cash prize) are conferred in a year.
Questions
1. What is patending?
2. What is the issue of patending?
3. What is PPVFR act?
4. Explain Plant Genome Savior "Farmer Reward" and "Farmer Recognition".
5. Expand EDV
References
253
1. Elizabeth, V. 2007. Law of plant Varieties protection, Eastern Book Company.
2. National Research Council. 1993. Global Dimensions of Intellectual Property
Rights in Science and Technology. Washington, DC: The National Academies
Press. https://doi.org/10.17226/2054
Marker Assisted Breeding and QTL L-34
A molecular marker is a DNA sequence that is readily detected and whose

inheritance can easily be monitored. The use of molecular markers is based on
naturally occurring DNA polymorphism, which forms the basis for designing
strategies to exploit for applied purposes.
Marker Assisted Breeding is a method of selecting desirable individuals in

a breeding scheme based on DNA molecular marker patterns instead of, or in addition
to, their trait values. A tool that can help plant breeders select more efficiently for
desirable traits.
Recently, the DNA markers have become the marker of choice for the study of
crop genetic diversity has changed the plant biotechnology. Techniques are being
developed to more precisely, quickly and cheaply assess genetic variation. With the
use of molecular markers technique, it is now possible to hasten the transfer of
desirable genes among varieties and to introgress novel genes from related wild
species. Polygenic characters which were previously very difficult to analyse using
traditional plant breeding methods, would now be easily tagged using molecular
markers. It would also be possible to establish genetic relationships between sexually
incompatible crop plants.
Techniques in assisting selction of desirable charcters involves the use of

molecular markers such as Random Amplified Polymorphic DNAs ( RAPDs),
Restriction Fragment Length Polymorphisms( RFLPs), microsatellites and PCR-
based DNA markers sch as Sequence Characterized Amplified Regions( SCARs),
Sequence Tagged Sites( STs) and Inter- Simple Sequence Repeat amplification(
254
ISSR), Amplified Fragment Length Polymorphic DNA‘s ( AFLPs) and Amplicon

Length Polymorphisms( ALPs) using F2 and back-cross populations, near –isogenic
lines, doubled haploids and recombinant inbred lined.
Identification of molecular markers linked to disease resistance genes

facilitates marker-assisted selection( MAS) for achieving gene combinations in
breeding programs. Various molecular marker systems such as RFLP, RAPD, ISSR,
AFLP and microsatellites( SSRs) have been widely used to tag resistance genes in all
the crops.
Identification of markers linked to disease resistance in Tomato
CROP PATHOGEN GENE IDENTIFIED TYPE OF MARKER

Tomato Meloidogyne incognita Mi RAP
Cladosporium fulvum Cf9 D
Leveillula taurica Lv AFL
Phytophthora infestans Ph P
Verticillium dahliae 2 RFLP,
Yellow leaf curl virus Ve RAPD RFLP
Ty1 RAP
D
RFL
P
 Genetic transformation in tomato by the vector

Agrobacterium tumefaciens, electroporation and direct gene transfer.
 Possible to produce somatic hybrids between S. lycopersicum and L.
peruvianum by protoplast fusion in tissue culture.
 Molecular genetic markers like RFLP, RAPD are used.
 Genetic engineering utilization for development of transgenic
tomato for disease and insect pest resistance and slow or delayed fruit ripening
tomato.
 Bt genes transgenic tomato – first developed and tested by
Monsanto in 1989.
 Transgenics with insecticidal properties, like delta-
endotoxin of Bacillus thuringiensis (Bt) protienase
inhibitors
 In India, gene cry AC was introduced into tomato to produce
transgenic resistant to Helicoverpa armigera.
 In India, Parker, Bonus, VFN-8 possesses resistance against
255
H. armigera
 Genotype 122775 was found resistant against H. armigera

 Bt genes (Gui ai, G42Aa (cry AB)) are being utilized to develop
transgenics with resistance to fruit borer.
 Coat protein(cp) from the viral genome TMV( Tobacco mosaic
virus) and CMV(cucumber mosaic virus).
 Genetic mapping and QTL (quantitative trait loci) analysis by
RFLP technology.
 ( fw 2.2) - One of the major genes in tomato domestication.
 Recently genetically engineered male sterility
system have been developed in tomato Cytoplasm(L.
peruvianum) and L. pennelii
Genetic engineering in Capsicum
The coat protein (CP) gene of cucumber mosaic virus (CMV) was cloned from a
Chinese CMV isolate, the CMV promoter and NOS terminator added and the gene construct
was transformed into both sweet pepper and tomato plants to confer resistance to CMV.
Safety assessments of these genetically modified (GM) plants were conducted. It was found
that these two GM products showed no genotoxicity.
Molecular markers for colour improvement in onion
 Bulb colour is one of the important traits in onion (A. cepa L). Three major colours of
white, yellow, red and a variety of other bulb colours such as chartreuse and gold
exist in onion germplasm.
 The bulb colour is due to flavonoid compounds and 54 kinds of flavonoids have been
reported in onion.
 Critical mutations in the chalconeisomerase (CHI) gene causing gold onions were
identified.
 The inactivation of dihydroflavonol 4-reductase (DFR) in the anthocyanin

synthesis pathway was responsible for colour differences between yellow and red
onions, and two recessive alleles of the anthocyanidin synthase (ANS) gene were
responsible for a pink bulb colour .
 Based on mutations in recessive alleles of these two genes PCR based markers were
developed for identification of polymorphisms between pink and red alleles of the
ANS gene. Most pink onions were homozygous recessive for the ANS gene
indicating the homozygous recessive. The two pink onions, heterozygous for the
ANS gene, were also heterozygous for the dihydroflavonol 4-reductase (DFR) gene
indicating that the pink colour was produced by incomplete dominance of a red
colour gene over that of yellow colour.
256
 Functional CAPS markers were developed for two inactive DFR-A alleles, DFR-APS
and DFR-ADEL, for detection of inactive DFR-A alleles responsible for a failure of
anthocyanin production in onions. Of these two alleles, DFR-APS predominantly
occurs in yellow onion cultivars.
Questions
1. What is QTL?
2. What is MAB and MAS?
3. Write in detail about identification of markers linked to disease resistance in

Tomato.
4. Write in detail about molecular markers for colour improvement in onion.
5. Write in detail about molecular markers?
References
1. Boopathi and N Manikanda.2013. Genetic Mapping and Marker Assisted

Selection:Basics, Practice and Benefits Springer Nature Switzerland AG. Springer is
part of Springer Nature.
2. Singh, B.D. and Singh, A.K . 2015. Marker-Assisted Plant Breeding: Principles
and Practices Springer Nature Switzerland AG. Springer is part of Springer Nature .

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VSC 611 BREEDING OF VEGETABLE

Course Content Prepared by

Dr. T.Uma Maheswari

To impart knowledge on principles and practices of breeding of

Origin- botany- taxonomy- cytogenetics- genetics- breeding

Unit I Breeding of solanaceous vegetables

Tomato, brinjal, chilli and sweet pepper.

Unit II Breeding of cucurbitaceous vegetables

Unit III Cool season vegetables

Cabbage, cauliflower, carrot, beet root and radish.

Unit IV Bulb and tuber crops

Onion, garlic, potato, tapioca and sweet potato.

Unit V Greens and beans

Okra, moringa, peas, beans and amaranthus.

Modes of pollination and reproduction- pollen morphology and

Origin- botany and taxonomy- genetics- cytogenetics- plant

1. Study of pollination mechanisms, pollen morphology and viability

Solanum pimpinellifolium S.cheesmaniae S.habrochaites

All these species of Solanum and a few others have either

Solanum lycopersicum var pyriforme Pear shaped tomato

No fruit formation a,f,hl,

Light green foliage Lg

Non ripening Nor

3) HYBRIDIZATION( Heterosis breeding)

 Arka Vardan: IHR-550-3 X IHR-932

 Arka Shreshta: 15-SBSB X IHR-1614

4) BACK CROSS METHOD

5) HYBRIDIZATION FOLLOWED BY PEDIGREE SELECTION

 Include disease and pest resistance.

DISEASE RESISTANCE SOURCE RESISTANT VARIETIES

Buckeye rot S. pimpinellifolium Early Selection,KT-10,

Late Blight S. pimpinellifolium Ottawa 30, Ottawa 31,

Include environmental conditions such as;

 Low and high temperature

A few species tolerant to abiotic stresses are

S. cheesamanii Salt tolerance, heat tolerance for

Abiotic stress resistant varieties

Pusa Sadabahar Fruit set at both low (6o C) and high

Sabour Suphala Salt tolerant at seed germination stage

Arka Vikas Tolerant to moisture stress

VARIETY ORGANIZATIO PARENTAGE FEATURES

HS 101 HAU , Hisar Sel. 2-3 X An exotic A very promising

Arka Saurabh IIHR , Bangalore Selection from a Semi determinate,

Pant Bahar GBPUA&T.Patna Bushy branched,

Century Century-12, Maitri, Rishi

Karnataka, Mangla, Vaishali,

MTH-1, MTH-2, MTH-6, MTH-15, MTH-

NS-386, NS-815, Summerset, Cross

Nath NA-501, NA-601

Nijjar NH-15, NH-25, NH-38

Sandoz Learika, Rasika, Avinash 11

Sungrow Arjuna, Krishna, Bhim

Sutton Sutton Grom, Prolific

Beejo Sheetal BSS-39, BSS-20, BSS-40, BSS-90

Arka Abha Arka Abhijit

Arka Raskshak Arka Samrat

Arka Saurab Arka Vikas

TNAU Tomato Hybrid CO3 PKM 1

Technology of production of hybrid seed