To In-Vitro Antispasmodic Activity of Aqueous Leaves Extract of Coriandrum Sativum (C. Sativum) Linn. On Chicken Ileum

Volume 8, Issue 5, May 2023 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
To In-vitro Antispasmodic Activity of Aqueous

Leaves Extract of Coriandrum sativum (C. sativum)
Linn. on Chicken Ileum
Dr. Panaskar A.N; Dr. Panaskar B. A; Mr. Jadhav A.R. Mr. Godage A.I; Mr. Kale R.P& Ms. Kale S.D
Abstract:- Coriandrum sativum (C. sativum) is a is the ostensibly increased risk of GI illnesses that overlap
medicinal plant traditionally known for its that are connected to reflux. The regurgitation of lower GIT
antispasmodic properties. In this study, we aimed to contents into the upper region of the GIT is referred to as
evaluate the in-vitro antispasmodic activity of an reflux. IBS and functional dyspepsia have both been linked
aqueous extract of C. sativum leaves on the chicken to disturbed motility. The link between functional dyspepsia,
ileum. A tissue bath model was used to determine the ulcerative colitis, and IBD and gastro esophageal reflux
potency of the extract in inhibiting acetylcholine-induced disease (GERD) has been confirmed by reports from various
contractions. The antispasmodic activity of the extract research [4,5].
was compared with that of standard antispasmodic
agents such as atropine. Phytochemical analysis was also Researchers have found that functional dyspepsia,
conducted to identify the active constituents responsible peptic ulcers, and IBS all have fluctuating catecholamine
for the antispasmodic activity. The results showed that levels. Additionally, other studies have discovered that IBS
the aqueous extract of C. sativum leaves exhibited and colitis have disturbed blood viscosity, which causes an
significant antispasmodic activity on the chicken ileum. imbalance in myogenic chemo metric auto regulation and
The extract was found to be more potent than atropine the stagnation of the abdominal circulation [6,7].
in inhibiting acetylcholine-induced contractions. The
phytochemical analysis revealed the presence of II. MATERIALS AND METHODS
alkaloids, flavonoids, and tannins, which may be A. Plant Collection, Authentication and Extraction:
responsible for the antispasmodic activity of the extract.
These findings suggest that C. sativum could be a  Plant material
potential source of antispasmodic agents for the The fresh leaves of corriandrum sativum were collected
treatment of gastrointestinal disorders associated with in and around Atpadi, Maharashtra after the authentication
increased intestinal spasms. by Prof. P. Yadav Department of Botany, Ishwarrao More
Patil Arts, Commerce & Science Mahila Mahavidyalaya,
Keywords:- Antispasmodic activity, Coriandrum sativum, Ekta Nagar Dighanchi Atpadi. A voucher specimen has been
Aqueous Extract.
deposited at the museum of college.
I. INTRODUCTION  Preparation of extract
The majority of the world's youngsters typically Fresh leaves collected were cleaned, and then extracted
complain of gastrointestinal issues, which are prevalent sorts with water by using Soxhlet’s apparatus. Thereafter, the
of complaints. In addition to gastroenteritis and acute extract was concentrated by flash evaporator. The yield
gastrointestinal illnesses, these conditions also include obtained was found to be 18 %. The crude aq. extract was
functional abdominal pain, ulcerative colitis, irritable bowel stored in refrigerator below 100C for further studies.
syndrome (IBS), infantile colic, and constipation. Disorders
B. Isolation of chicken ileum
of the digestive system can result in a lower quality of life
The hunt for alternative tissues for biological testing was
and a higher risk of anxiety and depression [1]. These
spurred by the current limitations on investigations with
conditions are characterized by recurring or persistent
laboratory animals. It was suggested to use tissues from
abdominal pain, which in IBS may be exacerbated by
animals that are typically consumed as food, such as fish,
defecation or relieved by a change in bowel habits [2].Along
sheep, goats, and cattle.[8] In 20 tests, we looked at the ileum
with symptoms like pain, constipation, or diarrhoea, from chicks sacrificed for food as a potential replacement.
abnormalities of intestinal movement are also linked to IBS. Chickens have a lengthy, uniformly sized small intestine.
People who have diarrhoea frequently exhibit symptoms like
Three times as thick as the longitudinal muscles are the
loose, watery stools [3].
circular muscles. Fresh chicken intestine was obtained from
This review provides a thorough explanation of a meat market that was registered with the local government
gastrointestinal disorders, their pathogenesis, the use of and placed in a flask with 500 cc of "chick" solution before
bioactive substances produce from therapeutic plants, and being carried right away to the lab and kept refrigerated.
their antispasmodic potential. We also highlighted a group NaCl 118.4 mm, KCl 4.6 mm, CaCl2 2.0 mm, MgCl2 0.5
of 15 traditional medicinal herbs with a variety of mm, KH2PO4 1.2 mm, NaHCO3 25 mm, glucose 11.1 mm,
compounds and their pharmacological potency diarrhoea, and sucrose 13.2 mm make up the chick solution.
constipation, and indigestion. The first possible explanation
IJISRT23MAY1926 www.ijisrt.com 3698

ISSN No:-2456-2165
C. Anti-Spasmodic Activity Assay Procedure:-  The same approach was then used to record the same
 First, acetylcholine concentration-dependent responses concentration dependent responses of acetylcholine
were recorded using Sherrington's recording drum and a (Ach) for a stock-II solution that included a blend of
frontal writing lever (at doses of 0.1, 0.2, 0.3, 0.4, 0.5, Tyrode's solution and coriandrum sativum extract at a
and 0.6 ml). For proper recording of the reactions in the concentration of 1 mg/ml.
presence of plane Tyrode's solution as stock-I solution,  Last but not least, the same concentration-dependent
contact time of 60 sec. and baseline of 30sec time cycle responses of Ach for a stock-III solution of a mixture of
were used. Tyrode's solution and atropine (as a conventional
antispasmodic drug) were seen.
III. OBSERVATIONS AND RESULTS
Effect of Acetylcholine on excised Chicken ileum reflected an increase in spasmodic activity (response) with an increase in
dose as shown fig.1
Fig.1: Response Curves of Acetylcholine (Black kymograph)
Table 1: Dose Response Relationship Observations of Acetylcholine

Sr. No Drug Dose Response
(cm)
1 0.1ML 0.6CM
2 0.2ML 1CM
3 Acetylcholine 0.3ML 1.5CM
4 0.4ML 1.8CM
Acetylcholine induced spasm followed by treatment of aqueous extract of coriandrum sativum showed prominent
antispasmodic activity as depicted in Fig.2

ISSN No:-2456-2165
Fig. 2: Response Curves of Acetylcholine + Leaves Extract (Black kymograph)
Table 2: Dose Response Relationship Observations of Acetylcholine and Extract

Sr.No Drug Dose Response
(cm)
1 0.1ML 0.3CM
2 0.2ML 0.5CM
3 Acetylcholine+ Extract 0.3ML 0.8CM
4 0.4ML 1CM
Also treatment of aqueous extract of corriandrumsativum showed receptor blocking action (antispasmodic) as that of
standard agent on isolated chicken ileum as shown in Fig. No.3.

ISSN No:-2456-2165
Fig. 3: Response Curves of Leaves Extract (Black kymograph)
Table 3: Dose Response Relationship Observations of Aq. Extract

Sr.No Drug Dose Response
(cm)
1 0.1ML 0.2CM
2 0.2ML 0.5CM
3 Aq. Extract 0.3ML 0.7CM
4 (coriander) 0.4ML 0.9CM
Fig. 4: Comparative dose response relationship of Acetylcholine and aqueous leaves extract of Corriander sativum on excised
chicken ileum

ISSN No:-2456-2165
IV. DISCUSSION [6.] W. Ling, Y. Li, W. Jiang, Y. Sui, and H. L. Zhao,
“Common mechanism of pathogenesis in
According to the findings of the current study, gastrointestinal diseases implied by consistent
acetylcholine (Ach) alone promotes contraction of the efficacy of single Chinese medicine formula: a
excised chicken ileum; however, when given in combination PRISMA-compliant systematic review and
with an aqueous leaf extract of the plant Corriandru metaanalysis,” Medicine, vol. 94, no. 27, article
msativum, a substantial reduction in ileal contraction was e1111, 2015.
seen. This shown that Corriandrum sativum aqueous leaf [7.] K. Mitsuyama, “Therapeutic leukocytapheresis in
extract has strong spasmolytic (anti-spasmodic) effect by inflammatory bowel disease: clinical efficacy and
inhibiting cholinergic receptors. mechanisms of action,” Cytotherapy, vol. 11, no. 2,
pp. 229–237, 2007
V. CONCLUSION [8.] Haranath PS. Kolkata: 2009. ‘Changing Face of
It was determined from all the data collected during Pharmacology’ – Souvenir of 42nd Annual
the current investigation that the corriandrum sativum leaf Conference of Indian Pharmacological Society 10-12
extract in aqueous form displays promising anti-spasmodic December; pp. 43–4.
action. Also It was discovered that corriandrum sativum has [9.] [GoogleScholar]
comparatively less powerful spasmolytic efficacy than
atropine, a common anti-spasmodic drug. Due to the fact
that many anti-spasmodic medications on the market have
side effects like urinary hesitancy, urinary retention,
mydriasis, tachycardia, blurred vision, and hypersensitivity
reactions, Lantana camara, a drug of herbal origin with a
high level of safety and efficacy, may be an effective
replacement for current medications as well as a new
member of the antispasmodic family.
ACKNOWLEDGEMENT
The authors express their sense of gratitude towards

management of Padmini College of Pharmacy, Dighanchi
for providing all obligatory facilities necessary to carry out
present work. Also Principal Dr, A. N. Panaskar, deserve a
special mention for their timely suggestions.
REFERENCES
[1.] D. Anheyer, J. Frawley, A. K. Koch et al., “Herbal

medicines for gastrointestinal disorders in children
and adolescents: a systematic review,” Pediatrics, vol.
139, no. 6, article e20170062, 2017.
[2.] G. Holtmann, A. Shah, and M. Morrison,
“Pathophysiology of functional gastrointestinal
disorders: a holistic overview,” Digestive Diseases,
vol. 35, Supplement 1, pp. 5–13, 2017.
[3.] H. Sadraei, M. Ghanadian, G. Asghari, E. Madadi,
and N. Azali, “Antispasmodic and antidiarrhoeal
activities of 6-(4-hydroxy-3-methoxyphenyl)-
hexanonic acid from Pycno-cyclaspinosaDecne.
exBoiss,” Research in Pharmaceutical Sciences, vol.
9, p. 279, 2014.
[4.] Y.-C. Lee, W. Y. Shau, C. H. Chang, S. T. Chen, M.
S. Lin, and M. S. Lai, “Antidepressant use and the
risk of upper gastrointestinal bleeding in psychiatric
patients:a nationwidecohort study in Taiwan,” Journal
of Clinical Psychopharmacology, vol. 32, no. 4, pp.
518–524, 2012.
[5.] S. S. Yarandi and J. Christie, “Functional dyspepsia
in review: pathophysiology and challenges in the
diagnosis and management due to coexisting
gastroesophageal reflux disease and irritable bowel
syndrome,” Gastroenterology Research and Practice,
vol. 2013, Article ID 351086, 8 pages, 2013

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Volume 8, Issue 5, May 2023 International Journal of Innovative Science and Research Technology

To In-vitro Antispasmodic Activity of Aqueous

IJISRT23MAY1926 www.ijisrt.com 3698

III. OBSERVATIONS AND RESULTS

Fig.1: Response Curves of Acetylcholine (Black kymograph)

Table 1: Dose Response Relationship Observations of Acetylcholine

IJISRT23MAY1926 www.ijisrt.com 3699

Fig. 2: Response Curves of Acetylcholine + Leaves Extract (Black kymograph)

Table 2: Dose Response Relationship Observations of Acetylcholine and Extract

IJISRT23MAY1926 www.ijisrt.com 3700

Fig. 3: Response Curves of Leaves Extract (Black kymograph)

Table 3: Dose Response Relationship Observations of Aq. Extract

IJISRT23MAY1926 www.ijisrt.com 3701

The authors express their sense of gratitude towards

[1.] D. Anheyer, J. Frawley, A. K. Koch et al., “Herbal

IJISRT23MAY1926 www.ijisrt.com 3702

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