Exam Questions 2022-23
Exam Questions 2022-23
Exam Questions 2022-23
Republic of Tajikistan
Ministry of Education and Sciences of Tajikistan
SEI “Avicenna Tajik State Medical University”
BIOCHEMISTRY DEPARTMENT
EXAM QUESTIONS
ON BIOLOGICAL CHEMISTRY
Exam questions
ENZYMES
41. General information about enzymes and their importance in the vital activity of
organisms. Proenzymes.
42. The chemical nature of enzymes and their general properties. Simple and complex
enzyme.
43. Methods for isolation and purification of enzymes.
44. Relative and absolute specificity of the enzyme.
45. Formation of an active site of enzyme. The main properties of enzymes and the
mechanism of their action.
46. Non-competitive inhibitors, mechanisms of action and their using in medicine. Their
difference from competitive inhibitors.
47. The enzyme ALT, its coenzyme, reactions which catalize by this enzyme. Its normal
level in blood serum and diagnostic value.
48. Nomenclature and classification of enzymes. Their location in the cell.
49. Enzymopathy. Enzymodiagnostics. Enzymotherapy.
50. Use of enzymes as medications in medicine.
51. The effect of temperature on enzyme activity.
52. Competitive inhibition. Their use in medicine.
53. Creatine kinase (CK), its normal level in blood serum and diagnostic value.
54. Allosteric center of the enzyme and its participation in the regulation of enzyme activity.
55. The activity of urinary amylase (diastase) is 128 IU on Volgelmut. Causes and
consequences.
56. Activators and inhibitors of enzyme activity.
57. Enzymes used to diagnose heart diseases.
58. Proenzyme. Factors affecting the activity of enzymes.
59. Oxidoreductase class enzymes, description, structure and function.
60. Indicator enzymes, their representatives and diagnostic significance in medicine.
61. Albinism, its causes and consequences.
62. Enzymo therapy. The main ways of the use of enzymes in medicine.
63. Dependence of the reaction rate on the substrate.
64. Irreversible inhibition. Irreversible inhibitors as drugs.
65. Isoenzymes. The enzyme creatine kinase, its isoenzymes. The diagnostic value of the
determination of this enzyme in medicine.
66. General characteristics of the enzyme (protein) alpha-1-antitrypsin. The cause of
insufficiency and consequences.
67. Regulation of the catalytic activity of enzymes.
68. The dependence of the enzymatic reaction rate on temperature.
69. Multienzymes and isoenzymes. Examples.
VITAMINS
70. The concept of vitamins, their role in metabolism. Provitamins, anti-vitamins.
71. Classification of vitamins, hypovitaminosis, avitaminosis, hypervitaminosis. Their
causes and consequences.
72. Vitamins as antioxidants.
73. Pellagra disease. Causes and symptoms of pellagra.
74. General characteristics of vitamin B6. Coenzymes B6 and their function.
75. Vitamin B1 hypovitaminosis, causes and consequences. Description of this vitamin.
76. Water-soluble vitamins, their coenzymes and function
77. General characteristics of vitamin riboflavin. Its coenzymes and function.
78. Beri-beri disease. Causes and consequences. Characteristics of the vitamin with which
this disease is associated.
79. Exogenous avitaminosis. Causes of hypervitaminosis.
80. General information about vitamin B3. Function of coenzymes B3.
81. The necessity for the use of vitamins after prolonged use of antibiotics. The role of
vitamins in the body.
82. The cause of endogenous Avitaminosis. The biological significance of vitamins.
83. The cause and symptoms of xerophthalmia? Vitamin therapy.
84. Vitamin B2, structure, physico–chemical properties, dietary sources, daily requirement,
biochemical function, signs of hypo- and avitaminosis, use in medicine.
85. Vitamin C, structure, physico–chemical properties, dietary sources, daily requirement,
biochemical function, signs of hypo- and avitaminosis, use in medicine
86. Vitamin P, chemical nature, dietary sources, biochemical function, signs of defficiency,
use in medicine.
87. Vitamin B3 (nicotinamide), structure, physico–chemical properties, dietary sources,
daily requirement, biochemical function, signs of hypo- and avitaminosis, use in
medicine.
88. Vitamin "reproduction" or "antioxidant". Dietary sources, daily requirement, function.
89. General characteristics of vitamin B12, and biorole.
90. General characteristics of vitamin B5 and biorole.
91. Name the organ in which beta-carotene is converted into vitamin A. The chemical and
biological name of this vitamin and function.
92. Why is rickets observed in the absence of sunlight?
93. Iron deficiency Anemia. Causes and consequences.
94. Hypovitaminosis is associated with impaired digestion and fat absorption. Consequences
95. Hypovitaminosis A in the liver diseases. Description of vitamin A.
96. Recommendation of vitamin E intake under adverse conditions. Characteristic of
vitamin E.
97. Causes and consequences of a deficiency of B5 (PP) in the body.
98. General information about vitamin B9. Its biological role.
99. Which vitamins are synthesized by the intestinal microflora. Name these vitamins.
Explain their biological role.
100. General characteristics of vitamin C, biorole, hypovitaminosis.
101. With prolonged use of sulfonamides or antibiotics, a person may occur hypovitaminosis
B9. Cause and consequences. The general characteristic of this vitamin.
102. Provitamins of group D. Active forms of vitamins of group D. The role of renal and
hepatic hydroxylases in their formation.
103. General characteristics of ubiquinone. Biorole of ubiquinone.
104. Vitamins A and D are stored in the body at a level sufficient to maintain metabolism for
several weeks or even months. B complex vitamins should be used much more often.
The reason for this phenomenon. Biorole of vitamins A and D.
105. The main biochemical disorders in rickets, types of rickets, clinical manifestations.
106. The pyruvate dehydrogenase complex is a necessary step in the catabolism of most
monomers in the process of production energy from nutrients. Vitamins involved in the
work of this complex. Their coenzymes.
107. Vitamin-like substances, the concept, differences from vitamins.
108. With hypovitaminosis of which vitamin, three main signs are observed that distinguish
them from a lack of other vitamins. These signs and their explanation.
HORMONES
109. Vasopressin: the place of synthesis, the mechanism of action. A disease that occurs
when it is deficient.
110. High biological activity and the distant action of hormones.
111. Calcitonin, the place of synthesis, the mechanism of action. The lack of this hormone
leads to which diseases and consequences.
112. The mechanism of action of insulin on blood glucose levels. Insulin antagonists.
113. Explain why insulin is administered subcutaneously, but not orally.
114. Characteristics of thyroid hormones.
115. Cytosolic mechanism of hormonеs action.
116. Additional endocrine gland formed during pregnancy and its function.
117. Membrane-intracellular mechanism. Hormones acting on the membrane-intracellular
mechanism.
118. Liberins, statins, chemical nature, representatives, mechanism of regulatory action
119. Increased levels of 17-ketosteroids in urine. The reasons for this situation. The
diagnostic value of its identification.
120. General biological properties of hormones.
121. The body of women has significantly more fat content than men, less muscle
development. What do you think what is the reason?
122. Will the intensity of the synthesis of ACTH and corticosteroids change in a patient who
is injected with glucocorticoids for therapeutic purposes? What is the mechanism?
123. Which fatty acid is the precursor of all prostaglandins? Name the groups of
prostaglandins.
124. Classification of hormones according to chemical structure and biological functions.
125. Target cells and cellular hormone receptors. Structure of the cellular receptor.
126. Glucagon, chemical structure, mechanism of action, effect on metabolism.
127. Regulation by hormones of the mediated (membrane) mechanism of action.
128. The role of hormones in the regulation of the reproductive function of the body.
129. The mechanism of action of aldosterone and its biological role.
130. Thyroxine, chemical structure, mechanism of action, effect on metabolism
131. Cushing's disease, causes and clinical manifestations
132. Тhyroid dysfunction and its consequences.
133. Vasopressin, chemical structure, mechanism of action, effect on metabolism
BIOLOGICAL OXIDATION
134. Biological oxidation, its characteristics.
135. The concept of redox pairs of donor substrates and electron acceptors.
136. Structural organization of mitochondria. The main structural components of
mitochondria are the outer, inner membrane, and mitochondrial matrix. Mitochondrial
function.
137. The location of enzymes in the mitochondria and their function.
138. Dehydrogenases and primary receptors for hydrogen-NAD and flavoproteins, NADH-
dehydrogenases.
139. Cytochromes, cytochromoxidase, structure, biological function, their redox potential.
140. Ubiquinone, structure, biological function, the value of its redox potential
141. The concept of oxidative phosphorylation. The energy output in the respiratory chain
during the transfer of electrons and protons from hydrogen to oxygen.
142. General characteristics of the chemiosmotic hypothesis of oxidative phosphorylation.
143. Disorders of energy metabolism and hypoxic conditions.
144. The role of vitamins B3, B5, B2 and E in biological oxidation.
145. Diagram of the respiratory chain and its explanation.
CARBOHYDRATE METABOLISM
146. Anaerobic breakdown of carbohydrates in the body; its biological significance and its
end products and their fate in the body.
147. Glycolysis and glycogenolysis, their similarities and differences.
148. Glycolysis and alcoholic fermentation, similarities and differences.
149. The fate of lactic acid in the body as a product of the anaerobic breakdown of
carbohydrates. The reaction of the formation of lactic acid from pyruvate. Its enzymes
and coenzymes.
150. Glycogenolysis.The total equation of anaerobic glycogenolysis. Regulation of this
process.
151. Neuro-humoral regulation of the glycolysis process
152. Glucose phosphorylation reaction. The enzyme catalyzes it. Its humoral regulation.
153. Glycolytic oxidoreduction. Enzyme and its coenzyme.
154. The amount of pyruvate molecules formed during the glycolytic breakdown of glucose.
The formula of pyruvate. The amount of ATP is formed during the oxidation of
pyruvate.
155. Decomposition of glycogen in the liver during stress. Neuro- humoral regulation of this
process.
156. The number of pyruvate molecules during the breakdown of two glucose molecules.
Justify the answer. The further fate of Pyruvate in the body.
157. Neuro-humoral regulation of physiological hyperglycemia.
158. The total energy effect of anaerobic glycogenolysis. In what state of the body this
process takes place and its regulation.
159. Determine the number of oxidative reactions in the TCA. Energy effect of this cycle.
160. Dehydrogenation of the substrate and hydrogen oxidation as an energy source for ATP
synthesis.
161. The number of ATP molecules during aerobic glycogenolysis. Stages of aerobic
glycogenolysis.
162. Determine the number of non-oxidative reactions in the TCA cycle. Name them.
163. Oxidative decarboxylation of α-keto acid occurs in the TCA cycle. Enzymes, coenzymes
and vitamins involved in this process. The amount of ATP formed during this reaction.
164. Oxidative decarboxylation of pyruvate as a general pathway of catabolism, sequence of
reactions, structure of the pyruvate dehydrogenase complex.
165. Indicate schematically the sources of glucose synthesis during prolonged physical work.
166. Specify the number of dehydrogenation reactions during the aerobic breakdown of
glucose.
167. Direct glucose oxidation (pentose cycle) and its significance.
168. Uridine cycle of glucose metabolism. The synthesis scheme and the biorole of this
cycle.
169. Digestion and absorption of carbohydrates. Features of parietal digestion.
170. Similarities and differences between the effects of glucagon and adrenaline on
carbohydrate metabolism.
171. In some healthy people, after a sugar load, the glucose content in the blood may fall
below the initial level, which hormone does it depend on?
172. Define glucosuria, specify the renal threshold for glucose and in which conditions it is
observed.
173. List the main biochemical symptoms of diabetes mellitus and their characteristics.
174. Glycosylated hemoglobin. Its change in the blood in diabetes mellitus
175. Causes of acetonemia and acetonuria in diabetes mellitus.
176. Substrate included in the Krebs cycle. The final products of the TCA cycle. The
biological role of the tricarboxylic acid cycle.
177. Basic carbohydrates of animals, content in tissues, physiological role.
178. Substrate phosphorylation. It differs from oxidative phosphorylation.
179. After prolonged physical activity in students developed muscle pain. The reason for this
condition.
180. After the restoration of blood circulation in the damaged tissue, the accumulation of
lactate stops and the rate of glucose consumption decreases. Activation of what process
is caused by these metabolic shifts?
181. When running short distances, in untrained person obsreves muscle hypoxia. Which
metabolite does this lead to the accumulation in the muscles and why? The fate of this
product.
182. Ways of breaking down glycogen in muscles. Chemistry, energy, physiological
significance.
183. Possible ways of converting glucose-6-phosphate.
184. Show the main difference between glycolysis and glycogenolysis.
185. Show the main difference between substrate phosphorylation and oxidative
phosphorylation.
186. The Krebs cycle and its role in energy metabolism.
187. Glycogenosis, its types
188. The metabolism of galactose in the body and the violation of this process.
189. Metabolism of glucose in erythrocytes. Consequences of metabolic disorders in
erythrocytes.
190. Glycogenosis. Gierke's disease.
II Semester
LIPID MTABOLISM