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Assignmnet 6 - Solution

This document discusses the design of a continuous, well-mixed bubble column bioreactor used to convert tyrosine to DOPA using immobilized tyrosinase beads. Spherical beads 3mm in diameter are loaded in the reactor at 0.35 m3/m3. The generalized Thiele modulus and internal effectiveness factor are calculated to be 0.8991 and 0.71 respectively. The external effectiveness factor is 1 since mass transfer is negligible. The dilution rate is calculated to be 4.4366 x 10-5 s-1 and the required reactor volume to treat 25 m3 of tyrosine solution per day is 6.522 m3.

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267 views3 pages

Assignmnet 6 - Solution

This document discusses the design of a continuous, well-mixed bubble column bioreactor used to convert tyrosine to DOPA using immobilized tyrosinase beads. Spherical beads 3mm in diameter are loaded in the reactor at 0.35 m3/m3. The generalized Thiele modulus and internal effectiveness factor are calculated to be 0.8991 and 0.71 respectively. The external effectiveness factor is 1 since mass transfer is negligible. The dilution rate is calculated to be 4.4366 x 10-5 s-1 and the required reactor volume to treat 25 m3 of tyrosine solution per day is 6.522 m3.

Uploaded by

Rita
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© © All Rights Reserved
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Solution: Assignment 6

Bioreactor design and analysis – 2022

A continuous, well-mixed bubble column is used for the conversion of tyrosine to DOPA.
3-mm spherical beads are used to immobilise tyrosinase that catalyses conversion of
tyrosine to DOPA. The Michaelis constant for the immobilised enzyme is 5.76 gmol/m3.
20 gmol/m3 of tyrosine is fed into the reactor with desired conversion to be 97%. The
reactor is loaded with beads at a density of 0.35 m 3/m3; the entire enzyme is retained
within the reactor. The intrinsic Vmax for the immobilized enzyme is 1.285 x 10-2 gmol/s
per m3 beads. The effective diffusivity of tyrosine in the beads is 6.9 x 10-10 m2/ s; external
mass-transfer effects are negligible. Immobilisation stabilises the enzyme so that
deactivation is minimal over the operating period.

Given:
The reaction follows first order kinetics; Thiele modulus for a spherical bead
following first order kinetics is
𝐑 𝐤𝟏
𝛟𝟏 = √
𝟑 𝐃𝐀

Relationship between Generalized Thiele modulus and internal effectiveness


factor for a spherical catalyst following first order kinetics is given as

𝟏
ⴄ𝐢𝐥 = (𝟑𝛟𝟏 𝐜𝐨𝐭𝐡 𝟑𝛟𝟏 − 𝟏)
𝟑𝛟𝟐𝟏

6. Determine Generalized Thiele modulus for the spherical bead.

Diameter = 3 mm = 3 x 10-3 m
Radius R = 1.5 x 10-3 m
Michaelis constant, Km = 5.76 gmol/m3
Intrinsic Vmax = 1.285 x 10-2 gmol/s per m3 beads
Effective diffusivity, DA = 6.9 x 10-10 m2/ s
Vmax 1.285 x 10−2 gmol s −1 m−3
k1 = = = 2.231 x 10−3 s−1
Km 5.76 gmol m−3

R k
ϕ1 = √ 1
3 D A

1.5 ∗ 10−3 m 2.231 x 10−3 s −1


ϕ= √ = 𝟎. 𝟖𝟗𝟗𝟏
3 6.9 x 10−10 m2 s−1

7. Determine the internal effectiveness factor.


1
ⴄil = (3ϕ1 coth 3ϕ1 − 1)
3ϕ12

coth(x) is a hyperbolic function.


To calculate this value in a scientific calculator (Fx-100MS)
- Select hyperbolic function key [hyp] and then select [tan]. You will get the
function of [tanh].
- Now, [coth (x)] = 1/[tanh (x)] ]

1
ⴄil = [3(0.8991) coth 3(0.8991) − 1] = 𝟎. 𝟕𝟏
3(0.8991)2

8. Determine the external effectiveness factor.


Since external mass transfer is negligible, ηel = 1

9. Determine the dilution rate (in terms of s-1)


Initial Substrate, S0 = 20 gmol/m3
Conversion, X = 0.97
S0 − Sf
Conversion, X =
S0
gmol gmol
Sf = S0 − XS0 = S0 (1 − X) = 20 (1 − 0.97) = 0.6
m3 m3

ⴄT = ⴄil ⴄel = (0.71)(1)


Vmax S
V ′ = Actual reaction rate = ⴄT ( )
Km + S
(1.285 x 10−2 gmol s −1 m−3 )(0.6 gmol m−3 )
= 0.71 ( )
(5.76 gmol m−3 ) + (0.6 gmol m−3 )
= 8.607 x 10−4 gmol s−1 m−3

At steady state rate of transfer of substrate reaction rate,

𝐷(𝑆0 − 𝑆𝑓 ) = 𝑉 ′ = 8.607 x 10−4 gmol s−1 m−3

8.607 x 10−4 gmol s−1 m−3 8.607 x 10−4 gmol s−1 m−3
𝐷= = (20−0.6) 𝑔𝑚𝑜𝑙 𝑚−3
(𝑆0 −𝑆𝑓 )

= 4.4366 x 10−5 𝑠 −1

10. Determine the reactor volume (in terms of m3) needed to treat 25 m3 of tyrosine
solution per day.
𝐹 25 𝑚3 𝑠 1 𝑑𝑎𝑦
𝑉= = ∗ −5
∗ = 𝟔. 𝟓𝟐𝟐 𝒎𝟑
𝐷 𝑑𝑎𝑦 4.4366 x 10 24 ∗ 3600 𝑠

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