Kiwi Store SAB V001 Manual

Operating Manual
for
Liconic
Kiwi
Stores
SAB_V001_MANUAL
Important Notice
 2016 LiCONiC AG Liechtenstein, all rights reserved.
The Information contained in this document is subject to change without notice.
Information provided by LiCONiC AG is believed to be accurate and reliable. However, the

user is responsible for the proper and correct use of the product. No license is granted by
acquisition of the product for any patent or patent rights of LiCONiC. If the user does not
follow the instructions given in this manual, LiCONiC does not take any responsibility for
injuries or damages caused by the LiCONiC product.
Contact Address
LiCONiC AG
Industriestrasse 8
FL - 9493 Mauren
Principality of Liechtenstein
Telephone: +423 373 63 39

Telex: +423 373 53 59
E-mail: [email protected]
Internet: www.liconic.com
Before operating the instrument, the user must read and understand this
manual.
Installation and Servicing

Installation, servicing and reinstallation of the instrument shall only be performed by System
Integrators and / or service personnel authorized by LiCONiC AG.
LiCONiC regularly offers Operator Training Courses. We highly recommend the attendance
of such a course prior to working with a LiCONiC Instrument.
StoreX Series V001 2 RKi, 05.02.2016

Table of Contents
Introduction ......................................................................................................................... 6
1. About the Product and this Manual ..............................................................................6
1.1 Intended Use ............................................................................................................................... 6
1.2 Unintended Use ........................................................................................................................... 6
1.3 Product Identification ................................................................................................................... 6
1.4 Scope of Delivery ........................................................................................................................ 6
1.5 General ........................................................................................................................................ 7
1.6 Definitions .................................................................................................................................... 7
1.7 Target User Group....................................................................................................................... 7
1.8 Importance of the Safety Instructions .......................................................................................... 7
1.9 Labels .......................................................................................................................................... 8
1.10 Safety - Basics............................................................................................................................. 8
1.10.1 General Operating Hazards ......................................................................................... 8
1.10.2 Mechanical Hazards .................................................................................................... 8
1.10.3 Electrical Hazards........................................................................................................ 8
1.10.4 Chemical, Biological and Radioactive Hazards .......................................................... 8
1.10.5 Extreme Temperature Hazards .................................................................................... 9
1.10.6 Suffocation Hazard...................................................................................................... 9
1.11 General Safety Regulations......................................................................................................... 9
1.11.1 Legal Requirements..................................................................................................... 9
1.11.2 General Inspection and Maintenance Duties ............................................................. 10
1.11.3 Spare Parts to be used................................................................................................ 10
1.11.4 Modifications............................................................................................................. 10
General................................................................................................................................11
2. The Kiwi Store SAB Series ........................................................................................11
2.1 Kiwi Store SAB .......................................................................................................................... 11
2.2 Kiwi Store SAB Features ........................................................................................................... 11
2.3 Kiwi Store Configuration and Capacity Overview...................................................................... 12
Options ...............................................................................................................................14
3. Kiwi Store Configurations...........................................................................................14
3.1 Kiwi Store SAB ULT .................................................................................................................. 14
4. Climate of LiCONiC Kiwi Stores SAB.........................................................................15
4.1 Overview.................................................................................................................................... 15
4.2 Cooling....................................................................................................................................... 15
4.3 Temperature .............................................................................................................................. 15
4.4 Humidity..................................................................................................................................... 16
5. Backup solutions for Kiwi Stores SAB........................................................................16
5.1 Overview.................................................................................................................................... 16
5.2 Auxiliary Refrigeration DF.......................................................................................................... 17
5.2.1 Auxiliary Refrigeration Kiwi Store SAB DF ............................................................ 17
5.3 Auxiliary Refrigeration ULT........................................................................................................ 17
5.4 CO2 Backup cooling.................................................................................................................. 17
5.5 LN2 Backup cooling................................................................................................................... 18
Identification.......................................................................................................................19
5.6 Handheld 1D Barcode Scanner................................................................................................. 19
5.6.1 Supported Barcode Types.......................................................................................... 19
5.6.2 Barcode Scanner Specifications ................................................................................ 20
5.7 2D Code rack scanner............................................................................................................... 20
5.7.1 Supported 2D code Types ......................................................................................... 20
5.7.2 2D code scanner / reader Specifications.................................................................... 20
6. Kiwi Store SAB Cassettes..........................................................................................22
System Installation.............................................................................................................23
7. KiWi Store SAB installation........................................................................................23
7.1 Customer installation guide ....................................................................................................... 23

7.2 3D-View ..................................................................................................................................... 23
7.3 Top view .................................................................................................................................... 24
7.4 Panel Locations ......................................................................................................................... 25
7.5 General Requirements .............................................................................................................. 26
7.6 Clearance Requirements........................................................................................................... 26
8. Refrigeration ..............................................................................................................26
8.1 Refrigeration Elements Overview .............................................................................................. 26
8.2 Electrical Refrigeration Principles.............................................................................................. 27
8.3 Cold Water Cooler ..................................................................................................................... 27
8.4 Connections Cooler to Cold Store ............................................................................................. 28
8.5 Typical Water Cooler Integration ............................................................................................... 29
8.6 Cooling Water Connection......................................................................................................... 30
8.7 Requirements Cooling Water .................................................................................................... 31
8.8 LN2 Backup Connections .......................................................................................................... 32
8.9 LN2 Requirements..................................................................................................................... 32
9. Electrics.....................................................................................................................33
9.1 Overview.................................................................................................................................... 33
9.2 Electrical Connections Store ..................................................................................................... 33
9.3 Electrical Connections Cooling units ......................................................................................... 34
9.4 Electrical Connections Interface ................................................................................................ 35
9.5 Requirements ............................................................................................................................ 35
9.6 Gassing Installation ................................................................................................................... 36
9.6.1 Kiwi Store Gassing and Backup Options .................................................................. 36
9.6.2 Safety Information..................................................................................................... 36
9.6.3 Installation Safety Recommendations ....................................................................... 38
9.6.4 Gassing System Installation ...................................................................................... 41
9.6.5 Specific Advice for CO2 Carbon Dioxide................................................................. 42
9.6.6 Specific Advice for N2 Nitrogen............................................................................... 44
9.6.7 Principles of Operation of Gassing Options.............................................................. 45
9.6.8 Integrated Gassing Safety Features ........................................................................... 48
9.6.9 Backup Refrigeration installation.............................................................................. 50
9.6.10 Specific Advice for LN2 Liquid Nitrogen................................................................. 51
9.6.11 Refill LN2 Liquid Nitrogen cylinder......................................................................... 52
9.6.12 Refill LCO2 Liquid CO2........................................................................................... 53
Manual Operation ...............................................................................................................54
10. Alert System ..............................................................................................................54
10.1 SAB Alerts ................................................................................................................................. 54
11. Doors.........................................................................................................................54
11.1 User door ................................................................................................................................... 54
12. Cassettes...................................................................................................................55
13. Barcode Reader.........................................................................................................58
13.1 Handheld 1D Scanner for plates and Cryo Boxes..................................................................... 58
13.2 2D Scanner / Reader for tubes.................................................................................................. 58
14. Power-On SAB ..........................................................................................................59
15. Climate Settings.........................................................................................................60
15.1 Temperature Settings ................................................................................................................ 60
15.2 Humidity Settings....................................................................................................................... 60
16. Cooling unit operation................................................................................................60
16.1 Switch on cooling SAB DF......................................................................................................... 60
16.2 Switch on cooling SAB ULT....................................................................................................... 61
16.3 Recovery after Backup activation .............................................................................................. 63
16.3.1 Procedure................................................................................................................... 63
16.4 Service operation of backup cooling ......................................................................................... 64
16.4.1 Procedure................................................................................................................... 64
17. Routine Safety Checks ..............................................................................................65
Software..............................................................................................................................66
18. Sample Management Software..................................................................................66
18.1 Order File................................................................................................................................... 66
18.1.1 Plate retrieval............................................................................................................. 66

18.1.2 Sample retrieval......................................................................................................... 67
18.2 PlateExplorer ............................................................................................................................. 68
18.2.1 Plate content import................................................................................................... 68
18.2.2 Import ........................................................................................................................ 69
18.2.3 Export ........................................................................................................................ 73
18.2.4 Manual Operations .................................................................................................... 78
18.2.5 Layout of the PlateExplorer....................................................................................... 79
Safety Sheets......................................................................................................................81

Introduction
1. About the Product and this Manual
1.1 Intended Use

The LiCONiC Kiwi Store is intended for storage, identification and transfer of
Microplates and Tubes. It is to be applied exclusively in the research field. The
LiCONiC Kiwi Store is intended to work as a stand alone unit or an element in an
automated system.
1.2 Unintended Use

Due to its open architecture, the LiCONiC Kiwi Store is NOT intended for
regulated clinical and diagnostic applications (IvD Directive).
Read the safety instructions in this Manual carefully.
1.3 Product Identification

The Type Plate is located at the rear of the LiCONiC SAB
For future reference, please read the instrument Serial No. on the rear of your
instrument and write it in the provided space.
1.4 Scope of Delivery

• Basic Unit, consisting of a Cassette Lift, an antechamber and the storage
chamber
• Cooling unit for storage chamber
• Cooling unit for antechamber
• Product Documents
• Cables
• User Software and Drivers
• Cassettes (as specified)
• Redundant Cooling (only if ordered)
• LN2 Backup (only if ordered, does not include DEWAR)
Exact delivery scope may differ according to project scope

1.5 General
The LiCONiC Kiwi Store is a complete product for end users. Setup of equipment
is conducted by LiCONiC Engineers or by LiCONiC trained and certified
personnel.
1.6 Definitions
Operator: Any person who uses the equipment for its intended
purpose.
Authorized Engineer Authorized person responsible for installation, initial start

up. Person who carries out service and maintenance
tasks and is therefore contacted in the case of any
problems with the system.
Plate Any SBS-Format sample plate or tube rack.
Cryo Box Storage card board box for cryo tubes
Samples Any individual container containing sample material,

tubes, vials and so on.
1.7 Target User Group

The Kiwi Store has been conceived for applications in the research field.
Instrument operation requires a trained user.
1.8 Importance of the Safety Instructions

This chapter contains general information assuring safe operation of the
instrument. More specific instructions in regard to safety are given throughout this
manual, at the respective points where observation is most important.
Make sure that all Safety Instructions in this publication are strictly followed.

1.9 Labels
Throughout this publication, Warning Notices are to be interpreted as follows:
WARNING !
Indicates the possibility of severe personal injury, loss of life or equipment

damage if instructions are not followed.
CAUTION !
Indicates the possibility of severe equipment damage if instructions are not

followed.
NOTE !
Gives helpful information about the equipment.
DANGER !
May be harmful to your eyes.
1.10 Safety - Basics

1.10.1 General Operating Hazards
Carefully observe the following precautions:
1.10.2 Mechanical Hazards

Keep the housing and safety panels closed and never reach into the instrument
workspace when the unit is in operating mode. Ensure safety of user prior to any
tasks sent to the equipment.
1.10.3 Electrical Hazards

Keep the housing and safety panels closed. Before opening any of the panels
disconnect the power supply to avoid electrical shock.
1.10.4 Chemical, Biological and Radioactive Hazards

All samples and test-kit components must be considered as potentially
hazardous agents. Therefore, a potential risk may arise from the liquids being
handled, such as infectious biological samples, toxic or corrosive chemicals, or
radioactive substances. Strictly apply appropriate safety precautions according to
local, state and federal regulations.
Prior to any first time application, test runs should be made with a neutral liquid to
allow optimization of all liquid handling parameters.
Handling and disposing of waste has to be in accordance with all local, state and
federal environmental, health and safety laws and regulations.

Prior to executing any maintenance task on the instrument or sending it, or parts
of it for repair, the instrument or the parts have to be thoroughly decontaminated.
1.10.5 Extreme Temperature Hazards

All surfaces inside the storage chambers, ante chamber and some parts of the
refrigeration may be considered as potentially dangerous if touched without
sufficient protective clothing. All components externally to the chamber are
protected and are not able to be touched. Only if protective covers are removed
components with extreme cold or heat may be touchable.
Inside the chamber temperatures below zero degree Celsius are present.
Contact to any surfaces with bear skin should be avoided. Protective clothing has
to be worn when manual operations are performed inside the chamber.
1.10.6 Suffocation Hazard

When entering the service area of the Storage chamber a potential risk of
suffocation is present. A maximum of 15 minutes is given to stay in the service
are without a respirator. When entering the service area a O2 monitor has to be
worn under any circumstances. The monitor has to provide at least an adjustable
O2 low limit and an acoustic alert when O2 level is to low.
WARNING !
Inert gases are asphyxiates and will displace oxygen to produce localized
oxygen deficient atmospheres
Inert gases are odorless, colorless and tasteless
Inert gases give no warning and may cause death in few seconds
The Exhaust of the CO2 or LN2 backup cooling option needs to be lead out
of the building otherwise danger of suffocation is immanent
In their liquid state inert gases are extremely cold fluids which can cause
serious burns when in contact with the skin
A cryogenic spill often gives a low lying cloud of vapor that creeps along the
ground
Due to very specific local rules and regulations in different countries it is the
customers / owners responsibility to install suitable oxygen (O2) and carbon
dioxide (CO2) monitor systems which trigger alarms to protect operators in
and around the SAB store.
It is not allowed to work inside the system alone. A second person has to be
outside surveying the work inside and to be in permanent contact with a radio
with the person inside.
1.11 General Safety Regulations

1.11.1 Legal Requirements
Please consult the Manufacturer's Declaration, delivered with each unit by
LiCONiC AG, listing all applied directives and standards.
In Europe, the System Integrator must provide a Conformity Declaration upon

final installation of the entire system.

1.11.2 General Inspection and Maintenance Duties
Only an authorized Engineer shall carry out inspection, maintenance and repair
tasks.
1.11.3 Spare Parts to be used

Only original LiCONiC Spare Parts may be used. If other parts are used during
the normal warranty period, the manufacturer's guarantee may be invalidated.
1.11.4 Modifications
Modifications shall only be carried out by an authorized Engineer. LiCONiC AG
will not accept responsibility for any claim resulting from unauthorized
modification or alteration.

General
2. The Kiwi Store SAB Series
2.1 Kiwi Store SAB
The Kiwi Store SAB is the most compact storage with integrated cassette
handling that covers semi-automated plate and Cryo Box storage needs at –20°C
and –80°C. The Kiwi Store SAB Series offers dry climate storage.
The modular mechanical design in combination with extremely simple commands

eases the use of a Kiwi Store SAB in any environment. A growing number of
accessories are available for the Kiwi Store SAB Series.
2.2 Kiwi Store SAB Features

All Kiwi Store SAB units have compact dimensions. The Kiwi Store SAB has a
user front door, which allows comfortable and easy access to the ante chamber.
The antechamber is the operation space for the manual transfer of racks and
plates from any additional equipment or transport box into the actual storage
area. Exchangeable cassettes make the use of the Kiwi Store SAB extremely
flexible. Cassettes are available for all common plate types and Cryo Boxes.
Storage
Dryer
Antechamber; User
Access to load and
unload material
Main Controller
cabinet
Cooling storage
Front User Door for

access to the ante
chamber
Cooling antechamber

Dimensions for 864/1260 Configuration :
2.3 Kiwi Store Configuration and Capacity Overview

The Family of Kiwi Store SAB is structured by their capacity for storage cassettes
and their climate.
The Kiwi Stores SAB are Stand Alone for manual semi-automated operation.
Capacity, given in Cassettes

Kiwi Store SAB No. of SBS Cassettes Height of Cassettes
864 / 1260 864 1260 mm
864 / 1775 864 1775 mm
864 / 1960 864 1960 mm
1152 / 1260 1152 1260 mm
1152 / 1775 1152 1775 mm
1152 / 1960 1152 1960 mm
These capacities are an excerpt of the most commonly requested sizes. Further
Storage sizes are available on request. All Kiwi Stores can handle and store a
wide range of types of plates and racks with SBS standard format. The
corresponding capacity is only depending of the height of the racks and if the
racks / plates are stored in separate levels or stacked on each other. Special
formats such as cryo boxes and larger sizes are available upon request.
For further systems dimensions please contact LiCONiC AG. These Dimensions
are examples and are not binding.

Options
3. Kiwi Store Configurations
3.1 Kiwi Store SAB ULT

The Kiwi Store SAB is the most compact storage with integrated cassette
handling that covers semi-automated plate and Cryo Box storage needs at –20°C
and –80°C. The Kiwi Store SAB Series offers dry climate storage.
The modular mechanical design in combination with extremely simple commands

eases the use of a Kiwi Store SAB in any environment. A growing number of
accessories are available for the Kiwi Store SAB Series.
All Kiwi Store SAB units have compact dimensions. The Kiwi Store SAB has a
user front door which allows comfortable and easy access to the ante chamber.
The ante chamber is the operation space for the manual transfer of racks and
plates from any additional equipment or transport box into the actual storage
area. Exchangeable cassettes make the use of the Kiwi Store SAB extremely
flexible. Cassettes are available for all common plate types and Cryo Boxes.
Storage
Dryer
Antechamber; User
Access to load and
unload material
Main Controller
cabinet
Cooling storage
Front User Door for

access to the ante
chamber
Cooling antechamber

4. Climate of LiCONiC Kiwi Stores SAB
4.1 Overview
Name Temperature Humidity

DF Deep Freezer -20..00°C 02..45%RH
ULT Ultra low temperature -80°C n.a.
The KiWi SAB DF Stores are designated for the use as compound storage,
whereas the ULT version is mainly for Biobanking use.
4.2 Cooling
Within all units the air in the DF (-20°C) part, is actively dried by forcing the air
through an evaporator. This allows cooling and actively drying the air. Since the
evaporator gets covered with ice after a certain time these units have an
automatic defrost function. The collected water will be exhausted trough a drain.
For DF Storage units this does apply for the complete storage area. In case of
the ULT systems the active drying is done in the user access chamber, the ante
chamber and the service area.
4.3 Temperature
All units are equipped with a Temperature-Sensor. This allows displaying the
temperature inside the storage chamber. A very high quality sensor is used for
this purpose. Quality and accuracy of the sensor is maintained by the Swiss
Federal Office of Metrology and the Swiss Accreditation Service (see
specifications in next chapter).
Due to the limited temperature range this sensor is used in the ULT storage
systems to monitor and control the climate in the service area and the ante
chamber. To monitor all other temperature, such as parameters of cooling and
storage chamber temperature, thermocouples are used.

4.4 Humidity
All units are equipped with an rH-Sensor. This allows displaying the humidity
inside the storage chamber. A very high quality sensor is used for this purpose.
Quality and accuracy of the sensor is maintained by the Swiss Federal Office of
Metrology and the Swiss Accreditation Service.
The Humidity sensors used in the Kiwi Store series are suitable for extremely
high humidity as well as for extreme dryness. They incorporate a humidity and a
temperature sensor allowing a relative humidity (rH) readout.
Specifications
Measuring Range 0..100 % RH
Accuracy@23°C ±1.5 % RH
±1.3 °K Temp.
Resolution 12 Bit / 0.02%
12 Bit / 0.1°C
Sensor PT100 Temperature
C94 Humidity
Adjustment - E2-PROM
5. Backup solutions for Kiwi Stores SAB
5.1 Overview
Name Temperature Principle

Auxiliary Refrigeration DF -20..00°C 2nd Refrigeration
unit
Auxiliary Refrigeration ULT -80°C 2nd Refrigeration
unit
CO2 Backup -20°C Evaporator for
CO2
LN2 Backup -80°C Evaporator for
LN2
The Kiwi Store auxiliary refrigeration units mainly cover the case of a failure of
the main cooling unit whereas the gas backup solutions are made to cover power
outages.

5.2 Auxiliary Refrigeration DF
All Kiwi Store SAB units can be equipped with an auxiliary refrigeration.
5.2.1 Auxiliary Refrigeration Kiwi Store SAB DF

The Kiwi Store SAB have a refrigeration unit placed at the side of the system.
Therefore easily an additional refrigeration unit can be placed next to the main
refrigeration.
To provide the complete redundancy a second evaporator is installed inside of

the storage chamber. The auxiliary refrigeration is equal to the main refrigeration
and therefore can provide the same cooling performance as the main unit.
5.3 Auxiliary Refrigeration ULT

As for the DF units the ULT auxiliary refrigeration solution is realized with a
second refrigeration unit. The main unit is running all the time to maintain the
temperature. The auxiliary refrigeration takes over during failure of the main
cooling unit or to assist the main unit during cool down. The auxiliary cooling unit
is equipped with its own control an monitoring PLC and is therefore completely
independent of the main control.
5.4 CO2 Backup cooling

The CO2 Backup solution is mainly for the use in DF systems to maintain the
temperature during power failure. Furthermore the CO2 option could also be
used for backup on ULT units. The CO2 bottle is connected to a controlled inlet
to the chamber injecting CO2 to maintain cold.
This option includes the tubing and the control. This backup solution is fully
monitored to ensure the temperature to stay at –20°C. Also an exhaust is part of
this option. For ULT unit an adapted regulation is in use to ensure to stay below -
70°C.
If the CO2 backup cooling is coming into action large amounts of CO2 are
released through the options exhaust. Therefore the exhaust needs to be lead
out of the building to not endanger personnel working in the area of the system.
WARNING !
The Exhaust of the CO2 backup cooling option needs to be lead out of the
building otherwise danger of suffocation is immanent
ground

5.5 LN2 Backup cooling
The LN2 Backup solution is intended for the use in ULT systems to maintain the
temperature during power failure. A LN2 Dewar, or alternatively a direct
connection to a large storage tank, is connected to an additional evaporator
specially designed for cooling use with LN2.
This option includes the evaporator, the tubing and the control, the Dewar or the
connection to a storage tank is not included. The size of the Dewar is defined by
the desired backup time and the desired refill interval. This backup solution is
fully monitored to ensure the temperature is maintained. Also an exhaust is part
of this option.
If the LN2 backup cooling is coming into action large amounts of N2-Gas are
released through the options exhaust. Therefore the exhaust needs to be lead
out of the building to not endanger personnel working in the area of the system.
WARNING !
The Exhaust of the LN2 backup cooling option needs to be lead out of the
building otherwise danger of suffocation is immanent
ground

Identification
5.6 Handheld 1D Barcode Scanner
For Identification during import and export a wireless 1D barcode hand scanner is
used. The reader is connected directly to the controlling software and could be
used throughout the storing and retrieving process for rack/plate identification.
The reader does give acoustic feedbacks if the correct/know plate is scanned or
a different signal in case the wrong plate or an unknown plate has been scanned.
5.6.1 Supported Barcode Types

The following default barcode types are enabled :
• EAN 8/EAN 13 / UPC A/UPC E without ADD ON

• Interleaved 2/5 4 - 99 characters
• Standard Code 39 1 - 99 characters
• Code 128 1 - 99 characters
The following barcode types are disabled :
EAN 128, ISBT128, Code 93, Codabar, pharmaceutical codes, MSI, Code 11,
Code 16K, Code 49 and GS1 DataBar

5.6.2 Barcode Scanner Specifications
• Light Source: VLD
• Wavelength: 630 - 680nm
• Output: 0.9mW
• Class: Class II
EN 60825-1 / CDRH 21 CFR 1040
• Max Resolution: 0.076 mm (3 mils)
• Scan Rate: 35 ± 5 scans / sec
5.7 2D Code rack scanner

A 2D code rack scanner can be integrated into the Liconic software. It allows the
reading of 2D Codes of a whole rack at once. To read the 2D code the rack
needs to be placed manually onto the reader.
5.7.1 Supported 2D code Types

The optional 2D code reader recognizes the following 2D code types.
• DataMatrix
5.7.2 2D code scanner / reader Specifications

Ziath DataPaq A6 Rack Scanner (suitable outside of climate)
• Scanning Device: Customized flatbed scanner

• Compatible rack vendors
Thermo Matrix
Thermo Abgene
Thermo Nunc
Remp
Micronic
FluidX
…. And all other 2D coded tubes
• Compatible rack sizes: 96, 48, 24
• Export data to Excel, text, XML, rack images
• Database connectivity: SQL Server, Oracle, MySQL, Postgres, HSQL and
others [v1.23+]
• Fast Scanning — 5 seconds per rack
• Displays damaged/missing tubes
• One-click scan for any size or brand of tube
• Simple installer — no dongles required
• Multiple scanners/multiple configurations
• 110/220 v automatically selected
• Fully integratable into robotics platforms
Ziath DataPaq Express Rack Reader (suitable for placement in low temperature climate)
• Scanning Device: Customized camera reader

• Compatible rack vendors integrated
Thermo Matrix

Thermo Abgene
Thermo Nunc
Remp
Micronic
FluidX
…. And all other 2D coded tubes
• Compatible rack sizes 96, 48, 24
• Export data to Excel, text, XML, rack images
• Database connectivity: SQL Server, Oracle, MySQL, Postgres, HSQL and
others [v1.23+]
• Fast Scanning — 1 second image acquisition
• Displays damaged/missing tubes
• One-click scan for any size or brand of tube
• Simple installer — no dongles required
• Multiple scanners/multiple configurations
• 110/220 v automatically selected
• Fully integratable into robotics platforms
WARNING!
LiCONiC AG is doing there utmost to make sure that real sample location
matches the stores database by using barcode readers / scanners and guiding
SAB users during store and retrieve process.
But due to the semiautomatic nature of the SAB store the user / customer is
finally responsible for sample location by moving them into a particular cassette
and associated cassette level manually.

6. Kiwi Store SAB Cassettes
The total cassette capacity depends on the unit size. An example list of the
number of cassettes for some predefined units are given in the SAB table below :
Kiwi Store SAB No. of SBS Cassettes Height of Cassettes

864 / 1260 864 1260 mm
864 / 1775 864 1775 mm
864 / 1960 864 1960 mm
1152 / 1260 1152 1260 mm
1152 / 1775 1152 1775 mm
1152 / 1960 1152 1960 mm
The SAB cassettes are typically customized to optimize the storage space for the
given application. The cassettes are either designed to hold a specific plate / tube
rack type, cryo box type or the stacking of plates is applied to further optimize
storage capacity.
The Kiwi Store SAB can handle almost every plate in SBS format currently
available on the market. Specific formats can be realized upon request. For
optimum performance LiCONiC offers a variety of cassettes.

System Installation
7. KiWi Store SAB installation
The installation of the KiWi Store SAB series is only performed by Liconic
personnel and in rare cases from thoroughly trained representatives. These
systems need major on site installation and adjustments. In this manual only
preparations necessary to be done by the customer are described.
7.1 Customer installation guide

7.2 3D-View
Dryer
Cold Store
Store
Antechmaber
Cooler
Antechamber
Controller
Logic
Main Backup
Cooler Cooler

7.3 Top view
Insulation
Panels
Cold Store
–80°C
Cooler
Antechamber
400VAC
Main
Cooler
400 VAC
Main
Cooler
400 VAC
Antechamber
Aux. Cooler
400VAC

7.4 Panel Locations
3.2 Electrical
Connection Store
2.4 LN2
inlet and
exhaust
LN2
Store
Backup
Interface
Store Auxiliary
Main Cooling
Cooling
3.3 Electrical Connection

Cooling unit
2.6 Cooling Water

Connection

7.5 General Requirements
Requirement
Details
Ambient Temperature: max. 22 °C

Humidity: < 40%
Room height 4 m (If not specified differently)
Ground cooling 10W/m^2 (Cooling energy loss passed on to the ground)
Floor load Recommended 1'000 kg/m^2 (for smaller load specifications please consult
clearance recommendations with Liconic Applications team, case-by-case
approval required)
Point load 250 kg
Heat dissipation < 2kW
Flatness +/- 1mm per 1m
Accessability Room has to be accessible from 7am - 7pm for installation
Storage A room has to be supplied to lock in all tools and material
Communication RJ45 Connector
Access to Network
7.6 Clearance Requirements
8. Refrigeration
8.1 Refrigeration Elements Overview
Cold Store
–80°C
Main Cooler Backup Cooler

400 VAC LN2 Dewar 2
Backup Cooler
Aux. Cooler
LN2 Dewar 1
400 VAC

8.2 Electrical Refrigeration Principles
1 stage
Condenser
Water Cooler
Inlet
Water Cooler
Outlet
st
1 Stage R404a
nd
2 Stage R508b
8.3 Cold Water Cooler
External
water
Cold water in
typ. ~14°C
Room AC
STC cooler
unit
Liconic STC
Store STC Cold water
return typ. ~30°C

8.4 Connections Cooler to Cold Store
Auxiliary Cooler
Back plane
cold store
LN2 Inlet
LN2 Exhaust Main Cooler
Connections
Main Cooler
Connections
Dryer
Power In
Main Cooler
Main Cooler
Cold store
Power In
aux. Cooler
Aux. Cooler
Connections
auxiliary cooler

8.5 Typical Water Cooler Integration
Cooling water
return line
Cooling water inlet
Water shut-off
valve

8.6 Cooling Water Connection
Refrigeration
cooling water
IN ¾“
Refrigeration
cooling water
OUT ¾”

8.7 Requirements Cooling Water
Requirement
Details
Cooling Media: Water

Water quality: Tap water quality, clear
Suspended solids: None
Conductivity: 50-600 uS/cm
Hardness: < 8° dH
PH: 7.8
CO2 aggressive: None
Iron: < 0.3 mg/L
Manganese: < 0.05 mg/L
Sulfate: < 250 mg/L
Chloride: < 250 mg/L
COD: < 40 mg/L
Bacteria: < 1000 CFU/ml
Pressure Difference: 2.5 bar (p.e.: IN 5 bar; OUT 2.5 bar)
Max. Inlet Pressure: 10 bar
Temperature: IN 5 .. 18 °C
Flow: typ. 3-5 l/min @14°C
Temperature Return: 35°C
Connection fittings: 3/4" male
Individual connections for main and redundant cooling
Mixture of Water with Glycol are possible.
Typical pump used for
cooling loop
Redundant Cooling A backup water cooling is highly recommended.

Experience showed the vitality of the water cooling. If the water cooling is
facing an error both main and redundant cooling units are inhibited to work if
connected to the same circuit.
Therefore the installation of a redundant or fresh water cooling is
recommended.
Water Connections 6x ¾” male

Main: 2x Inlet; 2x Return
Auxiliary: 1x Inlet; 1x Drain/Return
Interface Compartment: 1x Inlet; 1x Return
Auxiliary cooling is highly recommended to be connected to second
(redundant) water circuit or fresh water supply.
Drain Interface Compartment and Storage Dryer may perform regular defrost cycles
Interface Compartment and Storage Dryer are equipped with a drain tube
leading the gathered water out of them.
Solution to remove the water may be foreseen. In the most simple case a
evaporation pan could be used. (Water would evaporate into the room over
time)
More sophisticated solution would be to provide a drain to guide the water out
of the room.

8.8 LN2 Backup Connections
Auxiliary Cooler
Chamber
cold store
Manual shut
off valve
Automatic
LN2 Inlet valve
LN2 Exhaust Main Cooler
8.9 LN2 Requirements

Requirement Details
Media LN2
Refill intervall Depending on DEWAR-Volume
With respect to the Leak rate the DEWAR has to be refilled to maintain the
minimum desired backup time.
Specifications based on Cryotherm Apollo 350:
24 h Back up = Consumption: 209 - 298 L
Refill: about every 28 - 80 days (recommended once a month)
Cooling time Depending on DEWAR-Volume
Specifications based on Cryotherm Apollo 350:
100% filled: typically 28 - 40 h
50% filled: typically 14 - 20 h
LN2 Consumption 7-10 kg/h = 8.7 - 12.4 L/h
Leak rate Depending on DEWAR
Specification based on Cryotherm Apollo 350:
0.5% / Day
Supply Pressure 1.5 bar
Supply Connection ½” SAE Male, Manual Shut Off Valve and automatic Valve after connection
N2-Exhauste Exhaust is necessary. In case of use of backup cooling
N2 will be released into the environment
The Exhaust has to be guided out of the building.
N2-Exhauste Connection ½” Open end copper tubing. A unidirectional valve is installed in the line to
prevent any air leaking into the LN2 Backup circuit

9. Electrics
9.1 Overview
Power 230VAC
Interface unit
Power 230VAC
UPS Store
Power 400VAC
Power 230VAC main cooler
non UPS Store
Power 400VAC
Power 400VAC auxiliary cooler
main cooler
9.2 Electrical Connections Store

9.3 Electrical Connections Cooling units
Communication
Power 400VAC
cooler
Main Power
switch
IEC 60309
3P+N+PE 400V / 16A
¾”

9.4 Electrical Connections Interface
9.5 Requirements
Requirement
Details
2x 380V / 50Hz 3 Phases

Supply Refrigeration Store 2x Fuse: 16A
2x Connector: CEE Type 611, 16A 5-Pol: 3xL, N, G (IEC 60309)
Phase sequence: clock wise
Supply Redundant 1x 380V / 50Hz 3 Phases
Refrigeration Store (optional) 1x Fuse: 16A
1x Connector: CEE Type 611, 16A 5-Pol: 3xL, N, G (IEC 60309)
Phase sequence: clock wise
Power consumption Typically:
Refrigeration Current: 8 - 10 A
Voltage: 380V / 50Hz 3 Phases
Power: 2.5 - 6 kW
Supply Logic Control Unit 230V / 50Hz UPS protection
Store Fuse: 10A
Connector: Type C14, 10A 3-Pol: L, N, G
Supply Dryer Store 230V / 50Hz
Fuse: 10A
Supply Interface 230V / 50Hz
compartment Climate Fuse: 16A
Power consumption Logic Typically:
Control Unit Store Voltage: 230V / 50Hz
Power: < 1 kW
Power consumption Dryer Typically:

Store Voltage: 230V / 50Hz
Power: < 2.5 kW
Power consumption Climate Typically:
Unit Interface Voltage: 230V / 50Hz
Power: < 2 kW

9.6 Gassing Installation
9.6.1 Kiwi Store Gassing and Backup Options
The Kiwi Store series supports a wide selection of gassing and backup options.
Typical properties of some commonly used options are described next. There are
other gassing options available. Note that each gassing and backup option is
linked to specific risks and hazards. It is important to be fully informed about any
specific application before installing the instrument or even running any
application.
9.6.2 Safety Information

General
Commonly used in research medicine are inert gases like: Nitrogen, Argon,
Helium and Carbon Dioxide. Inert gases are used mainly to create and maintain
inert atmospheres for an application or process. The above gases, which
generally don't react with other materials, displace oxygen in order to maintain an
inert, contaminant free atmosphere.
In their liquid form these gases are very cold, (the boiling point of liquid nitrogen
is -196° C) . Such cold liquids are known as cryogenic liquids, (from the Greek,
kryos meaning frost and gene meaning producing). The liquid gases therefore
can be used as backup cooling option in case of power failure.
Major hazards
There are some major hazards associated with these gases
WARNING !
Oxygen is the only gas, which supports life. The normal concentration in the air
that we breathe is approximately 21%. If the oxygen concentration in air
diminishes or, (what amounts to the same) if the concentration of inert gases
increases, a situation is rapidly reached where the hazards of asphyxia become
very great.
The danger occurs as soon as the oxygen levels are lower than 16%. Inert
gases are odorless, colorless and tasteless and are therefore a great deal more
dangerous than other gases such as chlorine, ammonia, or hydrogen sulfide,
which can be detected by their odor, at very low concentrations. The asphyxiating
effect of inert gases occurs without any preceding physiological signals which
could alert the victim. It takes place very rapidly: just a few dozen seconds with
very low oxygen content. Stated clearly "you do not notice that you are passing
out!"
The danger from the inert gases such as nitrogen, argon, helium, etc cannot be
stressed enough!! . Especially in this case of Asphyxia as there are no warning
signs. People also vary considerably in their reactions to oxygen deficiency.

WARNING!
A cryogenic spill often gives a low lying cloud of vapor that creeps along
the ground
When a cryogenic liquid is released into the atmosphere, it evaporates and forms
a dense cloud. As it is very cold it is heavy and hangs close to the ground. So it
will flow into any low lying areas, such as drains or pits. Therefore the first
warning of a cryogenic spill is often given by the presence of a low lying cloud of
vapor that creeps along the ground. These clouds can be dangerous, as their
temperature or composition will be unknown. Visibility within the cloud is very low
with the possibility of falling, perhaps into contact with the cryogenic liquid. If the
cloud is nitrogen or argon then it is very likely that the cloud does not contain
sufficient oxygen to support life. In this case unconsciousness could be
immediate and death could follow.
CAUTION!
When you see a cryogenic spill or a large vapor cloud notify qualified
personnel immediately
Shut off the source of liquid, by a REMOTELY controlled valve
Do not enter a vapor cloud caused by vaporizing cryogenic liquid
DO NOT SMOKE!
When you see a cryogenic spill or a large vapor cloud notify qualified personnel
immediately. Call the Fire Department in the case of a large liquid oxygen spill.
Have them stand by until the spillage is dispersed.
If possible shut off the source of liquid, by a remotely controlled valve if one is
fitted. Do not expose yourself to the hazard, such as high oxygen, combustible or
asphyxiating atmospheres or cryogenic liquid. Do not allow liquid oxygen to enter
drains or sewers. If so advised, shut down any air conditioning or ventilating
systems that may draw vapor or gas from the spill into other locations.
If it is essential to enter a cloud caused by nitrogen or argon (note: never enter

one that could be rich in oxygen), then wear a self contained breathing apparatus
provided you are trained and qualified to do so. A stand-by person, also wearing
breathing apparatus must keep you under surveillance. If the release is oxygen,
then your clothes could be saturated making them extremely flammable. If you
suspect that you have been contaminated with oxygen do not go near any source
of ignition for no less than 15 minutes. Ventilate your clothing; change if possible.
A fire may occur at any time in the oxygen cloud, should it come into contact with
highly combustible material.

General Safety Instructions
When working with inert gases prevent contact of liquid gas, cold vapors, or
"snow" with exposed skin. Prevent entrapment of liquid in closed systems. Use
only in well ventilated areas. Compressed gas cylinders contain gaseous and
liquid gas at extremely high pressure and should be handled with care. Use a
pressure-reducing regulator when connecting to lower pressure piping systems.
Secure cylinders when in use. Never use direct flame to heat a compressed gas
cylinder. Use a check valve to prevent backflow into a storage container. Avoid
dragging, rolling, or sliding the cylinders, even for a short distance. Use a suitable
hand truck. For additional handling recommendations on compressed gas
cylinders, consult the Compressed Gas Association Pamphlet P-1.
CAUTION!
Wear loose fitting gloves of impermeable material such as leather when
working with cold liquid, solid, or vapor
Safety glasses are recommended when handling high-pressure cylinders
and in areas where vapors are discharged
Set-up gas cylinder in a well ventilated area. Keep cylinder away from sources of
heat. Storage should not be in heavy traffic areas to prevent accidental knocking
over or damage from passing or falling objects. Valve caps should remain on
cylinders not connected for use. Segregate full and empty cylinders. Storage
areas should be free of combustible material. Avoid exposure to areas where salt
or other corrosive chemicals are present. Install carbon dioxide cylinders with the
valve end up. See Compressed Gas Association Pamphlet P-1 for additional
recommendations.
9.6.3 Installation Safety Recommendations

Preventive Measures
The very first preventive measure is to inform all persons who handle or who use
inert gases:
• Of the hazard represented by the refraction of oxygen in atmosphere

• Of the preventive means to be employed in handling the gases
• Of procedures to be observed should an accident occur
This information and training should be systematically and periodically reviewed

in order to maintain an awareness with regard to these hazards. In particular,
training in rescue work is fundamental since quickly improvised rescue, without
the respect of a strict procedure, often proves to be ineffective, if not catastrophic
(the rescue worker lacking foresight becomes a second victim). An annual
program of training and rescue drills is recommended.
Ventilation
Depending on the existing ventilation, amount of gas used and size of room
where the unit is installed and operated, ventilation may be required. A local
exhaust may be useful at the point of sources of possible gas vapors. Where low
lying areas are not naturally ventilated vents should be situated to avoid higher
than normal concentration. Hazards to be considered in confined spaces are:

• Limited access and exits
• Increased likelihood of gas accumulation through leaks
• Increased asphyxiation risk
• Fire and explosion hazard
To be considered as confined spaces are the inside rooms of a building,

laboratory rooms, machine pits, culverts, basement trenches for piping. When
working in confined spaces, it is advisable that operators and other personnel are
fully aware of the hazards of oxygen deficiency and adopt the following practices:
• A fully documented permit to work system

• Personal and fixed oxygen analyzers
• Self contained breathing apparatus available for use and all personnel fully
trained in its use
• Personnel harnesses and ropes
• Operation of the "buddy system"
• A system of fresh air ventilation and fume extraction
In the case of permanent presence of personnel in a room where there are risks
of oxygen depletion mixture, ventilation must be maintained with at least 30mJ
per hour per person. The volume of air introduced per hour must not be less than
twice the enclosed space in question. Ventilation should never be carried out with
pure oxygen, but solely with air.
Testing of Oxygen or Gas Content

At present, various types of oxygen analyzers are available, which are often
simple and reliable to operate. Such a monitor should at least have
• A readout of the actual gas concentration

• A settable alarm threshold value
• An alarm output with an acoustic and optical alarm connected
• An error output that shows malfunction of the monitor
Ideally the monitor should be placed so that it can be read inside, as well as from
outside, the room which has gas present. The selection of the type of apparatus
depends on the nature of the work in the place to be monitored (temperature,
multiple detectors, portable equipment, etc). The picture below shows such a
monitor for CO2.

Testing the oxygen content is desirable, although attention must be drawn to the
fact that an analyzer alone is not absolute protection. Such equipment can
always malfunction, unexpectedly be out of adjustment or detectors can be
improperly positioned. Testing of oxygen content should therefore only be
considered as an aid to the detection of a lack of oxygen.
Work Permit
For certain types of work, safety instructions and a special work procedure must
be set up in the form of a work permit
Personnel Protection
Depending on the type of work to be performed, and the layout of the premises,
the decision to provide additional protection for personnel may be taken on the
initiative of the person in charge. Such additional protection may involve:
• The use of notices warning against the presence of a hazard

• The placing of a person on watch outside the work area
• The use of personal atmosphere monitors
• The organization of an alarm system for rapid intervention in case of an
emergency
• The wearing of a harness so that the worker can be easily and rapidly
taken out of an enclosed space in the case of an emergency. Preferably
this harness is connected to a hoist to facilitate removing the victim. It is
very difficult for one man to lift up another in the absence of a hoist.
• The wearing of an autonomous breathing mask, to the exclusion of all
cartridge masks, which are ineffective in a case of lack of oxygen.
• The wearing of protective goggles and gloves when handling liquid
nitrogen to avoid burns
• The wearing of all other means of protection, such as safety shoes,
helmet, etc., depending on the circumstances
Considering the hazards mentioned above, it is essential to provide all those who
handle or use inert gases with all the information and training necessary
regarding safety instructions, means of prevention and procedures to be followed
to avoid accidents, as well as rescue procedures to be implemented in the event
of an accident.

9.6.4 Gassing System Installation
On installations preferably choose pressurized cylinders rather then gas wall
outlets. The use of cylinders will limit the amount of gas being spilled in case of
fatal failure. Whenever a wall outlet installation is chosen, make sure that there is
a remote shut off valve installed outside the room where the gas is used.
Place the cylinder in an easily accessible and visible place next to the system.
Remember that the cylinder will have to be replaced or refilled routinely. As the
operator will have to check the cylinder routinely it should be clearly visible to all
personnel during routine work.
The room should provide a glass door or a window that will allow a good view of
the instrument and gas connection from outside the room. Often major defects
can easily be detected by visible vapor clouds or icy tubes without the risk of
entering a contaminated room.
Before installation make sure that the maximum specified inlet pressure is never
exceeded. Always install a main shut-off valve that will cut gas flow as close to
the source as possible. Secure cylinder when in use.
WARNING!
Gas cylinders are under extremely high pressure that must be reduced
for use with a StoreX incubator
Use a pressure-reducing regulator when connecting to lower pressure
piping system
Never exceed maximum specified inlet pressure
Never connect a Kiwi gassing inlet directly to a pressurized cylinder or
wall outlet
Use a two stage pressure regulator with indicating valves. The high pressure
gauge should have an indicating range of 0 to ~200 bar (0..3000 psi) to monitor
the pressure or the gas source. For CO2 a scale of 0..100 bar (0..2000psi) is
sufficient. The low pressure gauge should have an indicating range of 0..4 bar
(0..60 psi) and will monitor the input pressure to the Kiwi Store. A flow meter is
recommend since it will show correct operation of the system.
Cylinder Pressure High Pressure

Gauge Regulator
Main Shut-off Valve ! Gas Flow Meter
High Pressure Gas Gas Outlet

Cylinder Adjustment Valve
Low Pressure
Regulator
High Pressure
Adjustment Screw
Low Pressure Gauge
Low Pressure pipe

Two-stage pressure regulators are available from gas suppliers or laboratory
accessories suppliers. The picture above shows an example of an installation.
There is a wide variety of different models available. Your equipment may look
different.
The gas inlet of the Kiwi Store is located on the back of the instrument. Attach the
low pressure hose securely to the fitting. Always use the security nut. Never
operate the unit without the security nut being tightly fit.
Security nut
KiWi Store gas inlet
Low pressure pipe
Use 4x6mm PUR, PVC, Neoprene or Nylon tube for the low pressure connection
or in case of liquid nitrogen backup option a cryogenic safety tubing. Do not use
Silicon or natural rubber tubing. Make the low pressure tube long enough to allow
a minimum of movement of the gas cylinder for cleaning and maintenance work.
For longer length of tubing, copper or other metal tubing should be considered.
Foresee flexible tubing next to the instrument.
Be prepared for the following gas consumption values. These values may vary
significantly over time since they depend on the instruments use.
9.6.5 Specific Advice for CO2 Carbon Dioxide

Principals of Operation
CO2 is mainly used as backup cooling for the KIWI DF STORES. CO2 allows to
maintain the temperature at –20°C.
WARNING !
Improper use of CO2 or improper installation of the CO2 operated equipment
may cause death
Read CO2 safety information and installation instructions (See CO2
Installation) carefully. If you are not familiar with the handling of CO2 and / or
safety precautions contact trained personnel.

Properties of Carbon Dioxide
Carbon dioxide is colorless and odorless as gas or liquid. It is stored In
containers under its own vapor pressure. If the pressure is suddenly relieved, the
liquid rapidly cools as it evaporates and sublimes, forming dry ice at -78.5°C (-
109.3°F)
Boiling Point @ 1 atm -78.5°C, -109.3°F

Freezing Point @ 1 atm -56.6°C, 76.0°F
Vapor Pressure @ 20°C 56.5 atm, 831 psi
Solubility in Water @ 20°C, 1 atm 87.8% vol.
Specific Gravity (air =1) @ 20°C, 1 atm 1.53
Health Hazard Data of Carbon Dioxide

Carbon dioxide is a minor but important constituent of the atmosphere, averaging
about 0.03% or 300 ppm by volume. At higher concentrations it affects the
respiratory rate. Additional symptoms are described below.
WARNING !
THRESHOLD LIMIT VALUE TLV = 5’000 ppm
TLV-STEL (Short Term Exposure Limit) = 15’000 ppm (=1.5% vol.)
Carbon dioxide does not support life and may produce immediately hazardous
atmospheres. At a concentration in excess of 1.5%, carbon dioxide may produce
hyperventilation, headaches, visual disturbances, tremor, loss of consciousness
and death. Symptoms of exposure In the concentration ranges of 1.5-5% may be
highly variable, but typical symptoms of carbon dioxide intoxication include the
following:
CO2 Concentration Symptoms

3-6% Headaches, dyspnea, perspiration, breathing increases
to approximately four times normal rate, symptoms of
intoxication become evident, and slight choking may be felt.
6-10% Headache, dyspnea, perspiration, tremors, visual
disturbance, characteristic sharp odor noticeable, very
labored breathing and ringing in the ears, judgment may be
impaired, followed within minutes by loss of consciousness.
Over 10% Unconsciousness occurs more rapidly above 10% level.
Prolonged exposure to high concentrations may eventually
result in death from asphyxiation.
If the concentration of carbon dioxide exceeds 10%, unconsciousness can occur

without warning, preventing self-rescue. At much higher concentrations, carbon
dioxide displaces the oxygen in air below levels necessary to support life.
Persons suffering from the toxic effect of carbon dioxide should be moved to
areas with normal atmosphere. Self contained breathing apparatus may be
necessary to prevent toxic exposure or asphyxiation of rescue workers. Assisted
respiration and supplemental oxygen should be given if the victim is not
breathing. Frozen tissues should be flooded or soaked with tepid water (105-
115F; 41-46C). Do not use hot water. Cryogenic burns which result in blistering
or deeper tissue freezing should be seen promptly by a physician.

9.6.6 Specific Advice for N2 Nitrogen
Properties of Nitrogen
Nitrogen is colorless, odorless and tasteless, as gas or liquid. It is nontoxic and
non-flammable at atmospheric temperatures and pressures. A specific gravity of
0.9669 makes nitrogen slightly lighter than air. Nitrogen is mainly found in the
atmosphere, where it accounts for 78.1% by volume of the air we breath. It is
stored In containers under its own vapor pressure.
Boiling Point @ 1 atm -195.8°C (-320.4°F)

Freezing Point @ 1 atm -209.9°C (-345.8°F)
Vapor Pressure @ 20°C 150 atm
Health Hazard Data of Nitrogen

Nitrogen is inert and does not sustain life. Nitrogen is nontoxic, but may cause
suffocation by displacing the oxygen in air.
Oxygen Concentration Symptoms

21 - 14% (<19.5%) Increasing pulse rate, tiredness, dizziness, drowsiness,
Oxygen excess salivation, diminished mental alertness
14 - 11% Oxygen Muscular coordination slightly disturbed, intellectual
performance becomes difficult, breathing and pulse rate
increased
11 – 8% Oxygen Possibility of headaches, nausea and vomiting, collapse
or loss of consciousness.
< 6% Oxygen: Unconsciousness occurs without warning and so quickly
that the individuals cannot help or protect themselves.
Convulsive movements, possible respiratory collapse
and death or brain damage
On sudden pressure release, liquid nitrogen rapidly cools as it evaporates and

sublimes, forming dry ice at extremely cold temperatures of –196°C (-320°F) at
atmospheric pressure. This can cause severe frost bite.
WARNING !
Nitrogen may cause suffocation by displacing the oxygen in air. Ensure
levels of at least 19.5% Oxygen
On evaporation Nitrogen forms dry ice at extremely cold temperatures of
–196°C (-320°F)
Nitrogen may cause mild skin irritation, moderate eye irritation and possible
gastric distress. Cause of chronic illness is not known.
On vaporization Nitrogen expands by a factor of 700; one liter of liquid nitrogen

becomes 0.7 cubic meter (24.6 cubic feet) of nitrogen gas. This can cause the
explosion of a sealed container, or it can displace oxygen in the room and cause
suffocation without warning. Nitrogen can also become oxygen enriched and
cause ordinarily noncombustible materials to burn rapidly.
In case of an accident evacuate all personnel from the affected area. Persons
suffering from lack of oxygen should be removed to fresh air. If victim is not

breathing, use trained personnel to administer a supplemental oxygen supply and
artificial respiration to support vital functions. If breathing is difficult, administer
oxygen. Victim and rescuers must seek immediate medical attention.
Ventilate area and attempt to shut off the release of gas by tightening the main
valve. If it is not possible to stop the release of gas, allow the gas to be released
there. If leaking from cylinder or its valve, contact your supplier. Monitor area for
a minimum of 19.5% oxygen content before allowing re-entry by personnel.
In case of frostbite, restore tissue to normal body temperature, 37°C (98.6°F),

followed by protection of the injured tissue from further damage and infection.
Immediately place the frostbitten part in warm water (not to exceed 40°C) or wrap
affected parts gently in blankets. Under no circumstances should the water be
over 44°C (112°F), nor should the frozen part be rubbed either before or after re-
warming. Remove or loosen clothing that may constrict blood circulation to the
frozen area. In case of massive exposure, remove clothing while showering the
area with warm water. Encourage victim to gently exercise the affected part while
being warmed. Call a physician. The patient should neither smoke, nor drink
alcohol.
9.6.7 Principles of Operation of Gassing Options

Continuous Flow System
The Continuous Flow System is the simplest implementation of the gassing
option. The use of this solution is limited to low-accuracy applications where the
incubation chamber has to be filled with a specific gas or where a gas inside the
chamber has to be displaced. In some applications pre-mixed gas may be used.
The gas flow is adjusted to a value that generates the specified atmosphere. This
value is determined experimentally by the user. In most cases the low costs of
this solution will quickly be compensated by the costs caused by the excessive
gas consumption and / or additional safety requirements needed in conjunction
with this solution.
A pressure limiter and a flow limiter protect the system from inlet pressure
fluctuations and make it relatively insensitive to the amount of input pressure.
This solution is adequate for very low demanding applications and for non toxic
gases. Note that a significant amount of gas may be emitted to the instrument’s
environment.
Gas Inlet Chamber
Pressure
Exhaust
Limiter
Flow Limiter
Flow Regulator
An exhaust channels the surplus amount of gas to a defined point. The exhaust
is usually connected to the slightly under-pressurized exhaust system of the
building. The flow regulator is combined with a visual flow indicator and a scale
calibrated in l/min.

Open Loop System
The Open Loop System uses a more intelligent system that limits gas flow to the
actual required amount. This solution is suitable for mid-accuracy applications
where the incubation chamber has to be filled with a specific gas or where a gas
has to displaced. In applications where a certain concentration has to be
maintained pre-mixed gases may be used. The advantage of this solution over
the continuous flow solution is its lower gas consumption while still maintaining
low costs.
The picture below explains the functioning of this system. At the gas inlet a
pressure limiter and a flow limiter protect the system from inlet pressure
fluctuations. This also makes the system relatively insensitive to the amount of
input pressure.
Flow Regulator
Chamber
Gas Inlet
Pressure
Limiter
Flow Limiter
Exhaust
Main CPU
Gate Switch
Glass Door Switch
The actual gas flow is controlled by a solenoid. The solenoid is controlled by the
main CPU. By sensing both, glass door switch and Gate switch, the CPU knows
when there is a user access or when the Gate is opened and reacts accordingly.
In fact the CPU holds three values that represent the gas needed to add. The
standby value maintains the gas concentration when there is no access and
compensates for minor leakages of the incubation chamber. The Gate Access
Value compensates the gas loss caused by a Gate access while the User Access
Constant allows recovery after opening the glass door. The CPU internally
calculates the actual amount of gas needed in a specific situation. Since this
system limits the gas consumption to a fairly low level the exhaust does not have
to be connected in most cases.

Closed Loop System
The Closed Loop System is the most advanced system that gives a very high
level of accuracy and maximum flexibility. The gas consumption is limited to the
actual required amount. This solution is suitable for high-accuracy applications
where the incubation chamber has to be filled with specific gas concentrations
that may even vary with time. In addition this solution provides an external and
remotely readable readout of the actual gas concentration inside the incubation
chamber. Gas concentration can be set or altered remotely.
Right at the inlet a pressure limiter protects the successive components against
overpressure, up to ~10 bar. The pressure limiter also makes the gassing option
relatively insensitive to the amount of inlet pressure. A flow limiter also limits the
gas flow to ~10 l/min. A solenoid controls the gas flow to the incubation chamber
in accordance with the gas concentration inside the chamber.
A second tubing system measures the gas concentration inside the incubation
chamber. A pump removes a small amount of gas from the incubation chamber
and feeds it to the gas sensor. A particle filter protects the pump and sensor from
contamination.
Flow Limiter
Solenoid
Pressure
Limiter
Chamber
Gas Inlet
Gas Sensor
Exhaust
Gas Pump
Gate Switch
Measurement
Tube
Glass Door
Display Switch
Particle
Main CPU Filter
The main CPU monitors the opening of the incubation chamber preventing
unwanted gas flow to the surrounding environment. The solenoid is therefore
only opened when the glass door is shut and the Gate has not been opened over
an excessive period of time.

9.6.8 Integrated Gassing Safety Features
There are a number of safety mechanisms implemented in the system application
that protect the user from unwanted exposure to gases. Even in the case of
failure or operation errors these mechanisms can ensure safe shut-off of the gas
inlet. The optimum arrangement should be selected according to the level of
accuracy, danger and specified settling time. The level of safety depends on the
type of gassing option.
The continuous flow system has a minimal of safety functions. The exhaust limits
gas leaking into the surrounding environment. A slight under pressure on the
exhaust will also lower uncontrolled gas flow into the surrounding environment.
The safety elements of the continuous flow system simply limit the gas flow and
protect against overpressure to some extent. This system may not be used in an
higher risk environment.
A significantly higher standard of safety is provided by the open loop. system. In

addition to the basic flow and pressure protection, the gas flow is controlled by
the system. This results in a significantly higher level of safety and in a reduction
of gas consumption.
The above flowchart lists the steps that are implemented to avoid unwanted gas
emissions. As a first step the gas is always shut-off when the glass door is open.
This will eliminate the risk of large amounts of gas exiting when the user door is
opened. The same applies to the Gate. However, because of its smaller size
some gas flow is allowed even when the Gate is open. A Gate timeout is
implemented cutting gas flow when the Gate has been opened for an excessive
period of time. An additional general safety step is the gassing timeout. A gassing
timeout will occur when the solenoid is opened over a limited period of time.
An even greater level of safety can be achieved by the closed loopsystem. By

actually measuring and displaying the gas concentration possible errors or
malfunctions can be detected by the system. If the specified gas concentration is
not reached within a certain time limit the gas flow is shut-off and a error flag is
set. A display will inform the operator as to the correct operation of the system.

The option of remote access to the gas concentration allows a simple tracing of
the gas concentration over long periods of time.
The safety features are summarized in a table given below.
Feature Comment F1 O2 C3
Pressure Limiter Limits internal pressure to some extent + + +
Gas Flow Limiter Limits maximum flow avoiding full gas flow + + +
Exhaust Channels exhaust gas + + -4
Gas Switch Turns gas flow on and off - + +
Door Monitoring Cuts gas flow when door opens - + +
Gate Monitoring Cuts gas flow when Gate is open over a - + +
certain period of time
Gas Flow Timeout Cuts gas when a continuous gas flow occurs - + +
over a long period of time
Gas Flow Lookup Controls gas flow according to system status - + +
Gas Control Controls gas flow according to measured gas - - +
concentration values
Gas Measuring Gas sensor measures actual gas - - +
concentration
Gas Display Gas concentration is displayed to user - - +
Gas Transmission Remote gas concentration readout - - +
WARNING!
Because of the high safety standards maintained within the Kiwi the highest
risk of failure or unwanted gas emission lies in the external installations
1
Continuous Flow System
2
Open Loop System
3
Closed Loop System
4
Available on request

9.6.9 Backup Refrigeration installation
On installations preferably choose pressurized liquid gas storage cylinders rather
then wall outlets. The use of cylinders will limit the amount of gas being spilled in
case of fatal failure. Whenever a wall outlet installation is chosen, make sure that
there is a remote shut off valve installed outside the room where the liquid is
used.
Place the cylinder in an easily accessible and visible place next to the system.
Remember that the cylinder will have to be replaced or refilled routinely. As the
operator will have to check the cylinder routinely it should be clearly visible to all
personnel during routine work.
The room should provide a glass door or a window that will allow a good view of
the instrument and gas connection from outside the room. Often major defects
can easily be detected by visible vapor clouds or icy tubes without the risk of
entering a contaminated room. If this is not possible a oxygen level meter with a
remote alarm needs to be installed creating a acoustic and optical alarm in case
of low oxygen level.
Before installation make sure that the maximum specified inlet pressure is never
exceeded. Always install a main shut-off valve that will cut gas flow as close to
the source as possible. Secure cylinder when in use.
WARNING!
Never operate a damaged liquid gas cylinder
Special pressure-reducing regulator may be required to operate a liquid
gas backup.
Never exceed maximum specified cylinder pressure
Always ensure tight fit of all connections.
Supplied connection for liquid gas backup is a ½” SAE male (outside thread). For
liquid gas cylinder such as Cryogenic cylinders, standard connection hose are
available.
Over Pressure
Shut-off Valve Outlet Release
Connection hose Refill Inlet shut off

valve
Pressure build up
circuit shut off valve
Pressure build up Cylinder Pressure

circuit shut off valve Gauge

9.6.10 Specific Advice for LN2 Liquid Nitrogen
Properties of Nitrogen
Nitrogen is colorless, odorless and tasteless, as gas or liquid. It is nontoxic and
non-flammable at atmospheric temperatures and pressures. A specific gravity of
0.9669 makes nitrogen slightly lighter than air. Nitrogen is mainly found in the
atmosphere, where it accounts for 78.1% by volume of the air we breath. It is
stored In containers under its own vapor pressure.
Boiling Point @ 1 atm -195.8°C (-320.4°F)

Freezing Point @ 1 atm -209.9°C (-345.8°F)
Vapor Pressure @ 20°C 150 atm
Health Hazard Data of Nitrogen

Nitrogen is inert and does not sustain life. Nitrogen is nontoxic, but may cause
suffocation by displacing the oxygen in air.
Oxygen Concentration Symptoms

21 - 14% (<19.5%) Increasing pulse rate, tiredness, dizziness, drowsiness,
Oxygen excess salivation, diminished mental alertness
14 - 11% Oxygen Muscular coordination slightly disturbed, intellectual
performance becomes difficult, breathing and pulse rate
increased
11 – 8% Oxygen Possibility of headaches, nausea and vomiting, collapse
or loss of consciousness.
< 6% Oxygen: Unconsciousness occurs without warning and so quickly
that the individuals cannot help or protect themselves.
Convulsive movements, possible respiratory collapse
and death or brain damage
Spills of liquid Nitrogen have extremely cold temperatures of –196°C (-320°F) at

atmospheric pressure. This can cause severe frost bite.
WARNING !
Nitrogen may cause suffocation by displacing the oxygen in air. Ensure
levels of at least 19.5% Oxygen
Liquid Nitrogen has extremely cold temperatures of –196°C (-320°F)
Contact with liquid Nitrogen may cause skin frost burns and severe eye
damages.
On vaporization Nitrogen expands by a factor of 700; one liter of liquid nitrogen

becomes 0.7 cubic meter (24.6 cubic feet) of nitrogen gas. This can cause the
explosion of a sealed container, or it can displace oxygen in the room and cause
suffocation without warning. Nitrogen can also become oxygen enriched and
cause ordinarily noncombustible materials to burn rapidly.
In case of an accident evacuate all personnel from the affected area. Persons
suffering from lack of oxygen should be removed to fresh air. If victim is not
breathing, use trained personnel to administer a supplemental oxygen supply and

artificial respiration to support vital functions. If breathing is difficult, administer
oxygen. Victim and rescuers must seek immediate medical attention.
Ventilate area and attempt to shut off the release of gas by tightening the main
valve. If it is not possible to stop the release of gas, allow the gas to be released
there. If leaking from cylinder or its valve, contact your supplier. Monitor area for
a minimum of 19.5% oxygen content before allowing re-entry by personnel.
In case of frostbite, restore tissue to normal body temperature, 37°C (98.6°F),

followed by protection of the injured tissue from further damage and infection.
Immediately place the frostbitten part in warm water (not to exceed 40°C) or wrap
affected parts gently in blankets. Under no circumstances should the water be
over 44°C (112°F), nor should the frozen part be rubbed either before or after re-
warming. Remove or loosen clothing that may constrict blood circulation to the
frozen area. In case of massive exposure, remove clothing while showering the
area with warm water. Encourage victim to gently exercise the affected part
while being warmed. Call a physician. The patient should neither smoke, nor
drink alcohol.
9.6.11 Refill LN2 Liquid Nitrogen cylinder

When ever working with liquid Nitrogen use of personal safety equipment is
required. Thermal gloves and protective glasses have to be worn at least.
Refill interval of LN2 cylinder is depending on the required minimum backup time
and the available LN2 cylinder volume. All cryogenic cylinder have a certain
leakage rate which will define the time after which the minimum filling level of the
tank is reached. The filling level is shown by a gauge mounted next to the
connection points on the cylinder. The actual design may vary depending on
cylinder manufacturer.
Before disconnecting the connection hose close the pressure builder valve, the
shut off valve on the cylinder and the shut off valve on the connection point on
the actual storage chamber. Use two suitable spanner to loosen the connection
on the chamber (or on the cylinder depending on refill procedure). Usually the
cylinder is then moved to a refill station and refilled with LN2. Depending on
cylinder manufacturer the process may vary. Please refer to the manufacturers
cylinder manual for further details.
To reconnect the cylinder with the system, proceed in the reverse way to the
disconnecting procedure. Connect back the hose. Open the main shut off valve
on the cylinder, open the shut off valve on the storage chamber and open the
valve on the pressure builder. The pressure builder valve has to stay opened in
order to ensure proper operation of the LN2 backup option.
WARNING!
Wear personnel protection when disconnecting, refilling and reconnecting
LN2 cylinder.
Ensure the shut off valves are closed when disconnecting the cylinder.
Refer to cylinder manufacture manual for exact refill procedure
CAUTION !
Shut off valve of pressure builder has to stay open as long as the cylinder
is connected to the storage.

9.6.12 Refill LCO2 Liquid CO2
The refill procedure of the CO2 backup cooling is a relatively simple process as it
only needs to be performed if the CO2 cylinder is empty. As the CO2 cylinder has
no loss over time it has no regular refill interval.
When replacing a cylinder, make sure the shut off valve on the CO2 cylinder and
on the unit are closed. Use a spanner to disconnect the pressure regulator from
the bottle. Put the new bottle in place and reconnect the pressure regulator.
Open the shut off valve of the cylinder and of the storage.
WARNING!
Ensure the shut off valves are closed when disconnecting the cylinder.

Manual Operation
10. Alert System
The KIWI STORE has a combined acoustic / visual alert system. An alert occurs
when one or more abnormal statuses occur or when interaction is required.
When the system is operating properly there is no alert. The various alerts are
distinguished by the alert signal duration and the pause between the signals.
10.1 SAB Alerts

The storage area of the bigger KIWI STORES does not allow manual access,
therefore the following alerts are defined:
Signal / Pause Alert Comment, Action

(time in seconds)
0.1 / 2.4 No Initialization StoreX not initialized. Press Multi
Function Switch for initialization.
11. Doors
11.1 User door

The user door opens on the left side of the instrument and should be kept closed
when ever possible. A spring loaded mechanism keeps the user door closed.
When door is opened it should be for as short as possible periods.
In order to prevent large amount of humidity introduced into the system, the user
door is heated. Note: Leaving the user door open for extended periods will cause
frost and ice inside the system.
Pull user door handle down
User door
Springloaded Mechanism
The user door is opened by pulling on the left edge or on the handlebar and may
be opened to an angle of almost 180 degrees.

Regularly inspect sealing performance of the user door. The magnetic seal must
keep the user door firmly closed over its whole surface. When the user door is
out of alignment with the user shield it must be re-aligned by an authorized
person. Note that gaps between the magnetic seal and user shield will cause
undesired air exchange and therefore condensation and will degrade the
temperature distribution.
CAUTION !
Use the door handle to open user door
Keep user door closed when it is not being accessed
Keep user door access to a minimum
The seal must firmly close user door
Keep the seal clean
WARNING !
It is dangerous to leave the user door open over long periods of time.
Avoid excessive condensation on the glass door
12. Cassettes
Customized SAB cassettes allow simple and comfortable loading and unloading
of storage samples. Once prepared, the samples can be quickly transferred into
the climate chamber of the Kiwi SAB unit by means of the cassette lifts installed.
There is a wide variety of cassette types available. Make sure that you work with
the proper cassette size that matches the type of plates or Cryo Boxes you are
using.
CAUTION !
Do not add, bend or remove support rails.
If cassettes with different pitch height are used within the same system,
make sure a prior removed cassette is put back in exactly the same position.
Failures being generated like that, will result in destinations not found or
crashes.
Only a trained System Integrator may alter the instrument configuration and
reset the lift accordingly.
The SAB cassettes (see picture below) are open at their front for loading.

To put a plate into the cassette, choose the level and slide the plate along the
rails towards the back of the cassette. Slightly lift the front of the plate to avoid
excessive wear of the retaining nozzles at the front end of the rails. When the
plates are loaded make sure that they are all pushed towards the back of the
cassette. Plates that jut out may cause collision with the handler and may result
in damage to the handler.
Cassettes must be treated with great care. They are aligned within 1mm when
leaving the factory. Misaligned cassettes must be returned for re-alignment.
Misaligned, tilted or bent cassettes may cause handling failures. Special care
must be taken when cassettes are fully loaded with plates.
Never apply force to the cassettes. Do not drop a cassette as mechanical shock
will misalign it. Never lift a cassette by its side walls. This may bend the side walls
of the cassette. Bent side walls cause the plates to block when being loaded or
unloaded.

Cassette Side Wall
The plates / tube racks or Cryo Boxes are presented to the operator by means of
the cassette lifts shown in the pictures below :
CAUTION !
Make sure that ALL plates are pushed to the back of the cassette. Plates
jutting out the front side of the cassette may cause collision with the cassette
lift.
Lift cassettes by their cassette handles only
Do not drop cassettes. Misaligned cassettes can cause handling errors
Do not autoclave cassettes with plastic rails. Use disinfecting solution for
sterilization. All stainless steel cassettes may be autoclaved.
Keep cassette clean. Particles and ice could prevent reliable handling.

13. Barcode Reader
13.1 Handheld 1D Scanner for plates and Cryo Boxes

A handheld Barcode Scanner for plate/rack identification is used on the Kiwi SAB
Store. This one is placed in the antechamber and used when importing/exporting.
The Barcodes must be placed on the shorter side of the plates and must face
towards the open side of the cassette. The barcodes should be parallel to the
plate’s lower edge. The barcode label should be centered both vertically and
horizontally. On higher plates (e.g. deep well plates) place the barcode further
down. Preferably barcode labels should be at the same height independent of the
plate height. Barcode reading reliability is improved when the barcode is not
covered by the plate’s lid.
WARNING !
Laser Class 2. Laser light - do not look into the beam.
DANGER !
May be harmful to your eyes.
Use barcode testing device to verify barcode quality. Quality Class A, B or C are
required. Identification of Class D is not guaranteed. For sufficient print quality
use barcodes printed by thermal transfer or photographic methods. The barcode
label surface should be mat and clean. Do not use yellowed, dirty or damaged
barcodes.
13.2 2D Scanner / Reader for tubes

The 2D Scanner / Reader for tube identification is located in the antechamber
and the loading needs to be done manually before importing / exporting material.
The 2D codes must be placed on the bottom of the tube. The 2D reader operates
either on a scanner or camera based principle. If a camera is used it requires
lighting by short flashes to take pictures. These flashes may be a potential trigger
for people with photosensitive epilepsy.
WARNING !
A few people may experience epileptic seizures when viewing flashing lights
or patterns. Avoid watching the 2D code reading for longer time.
Use barcode testing device to verify barcode quality. The code label surface
should to be mat and clean. Do not use dirty or damaged barcodes.

14. Power-On SAB
The instruments power is activated by the main switch. The instrument is turned
on by pressing the power switch located at the front of the instrument. The
climate portion of the instrument is activated independently. An alarm will sound.
If the green light of the power switch does not turn on, check the fuse in the
mains supply.
Service COM-Port
Power Switch
Multi Function Switch; NC
Indicator Light
Display Temperature;
Dryer Evaporator
Display Temperature;
Service Area
Display Humidity; Service

Area
The alarm indicates that the handling is not activated. In order to activate the
handling, this has to be triggered out of the controlling software.
The temperature inside the climate chamber is displayed on the temperature

display. The temperature display may not be used to set temperature. The
operating temperature is fixed and may not be adjusted at any time.

15. Climate Settings
15.1 Temperature Settings

System Temperature is pre set. Temperature should only be changed by a
qualified system administrator or LiCONiC engineer.
15.2 Humidity Settings

Humidity is kept as low as possible and is not adjustable.
16. Cooling unit operation
16.1 Switch on cooling SAB DF

The cooling unit should stay switched on all the time. All cables should be
connected before start up.
To switch on the cooling unit the main power switch needs to be turned into the
position stating ON. The cooling unit will immediately start on the auxiliary cooling
as the handling is not switched on. As soon as the SAB handling (and therefore
the PLC controlling the climate) is switched on the main cooling will take over.
The key switch has to be in automatic position (see Picture 1).
Status LED Key switch
Main power switch
Picture 1: Cooling unit front view

Main power supply socket
USB communication
RS232 communication
Hardware communication
Thermocouple Terminals
Picture 2: Cooling unit back view
Status LED Status Description

st
Green 1 Cooling stage All conditions are fulfilled to run
operating first cooling stage
Yellow 2nd cooling stage All conditions are fulfilled to run
operating the second cooling stage
Red ERROR One condition is fulfilled to
trigger an error.
CAUTION !
Do not disconnect any cables, except a LiCONiC engineer did advise to
do so.
All cooling units have to be permanently switched on.
The key switch has to stay in automatic position.
Ensure normal conditions (p.e.: Power and Water) before switch on the
cooling units.
16.2 Switch on cooling SAB ULT

The cooling units should stay switched on all the time. Initial power up of cooling
should be performed with switched off handling of store. All cables should be
connected before start up.
To switch on the cooling unit the main power switch needs to be turned into the
position stating ON. The key switch has to be in automatic position (see Picture
3). This has to be performed on both cooling units main and auxiliary.

Status LED Key switch
Main power switch
Picture 3: Cooling unit front view

The first cooling stage of the cooling unit will immediately start to operate. The
green status LED therefore indicates all conditions fulfilled to run first stage. After
a certain time the second cooling stage will kick in. The yellow status LED
therefore indicates all conditions to operate the second stage are fulfilled.
Main power supply socket
USB communication
RS232 communication
Hardware communication
Thermocouple Terminals
Picture 4: Cooling unit back view

In case of an error the red status LED will be lit. If the error conditions are
resolved check the section “Recovery after backup activation” for the procedure
to return in to normal operation.
Status LED Status Description

st
Green 1 Cooling stage All conditions are fulfilled to run
operating first cooling stage
Yellow 2nd cooling stage All conditions are fulfilled to run
operating the second cooling stage
Red ERROR One condition is fulfilled to
trigger an error.
CAUTION !
Do not disconnect any cables, except a LiCONiC engineer did advise to
do so.
All cooling units have to be permanently switched on.
The key switch has to stay in automatic position, for normal operation.
Ensure normal conditions (p.e.: Power and Water) before switch on the
cooling units.

16.3 Recovery after Backup activation
In the case the backup cascade was activated and the system is running on one
of the backup options a recovery might be necessary. The recovery procedure
should only be performed after normal conditions for main cooling are restored.
CAUTION !
Ensure normal conditions being restored to operate the main cooling
Ask for assistance from a LiCONiC engineer in case of insecurity about
normal conditions and performing the recovery procedure.
Observe cooling after recovery. Contact immediately a LiCONiC engineer
in case of repeated stop of cooling after recovery.
16.3.1 Procedure
SAB ULT System running on auxiliary cooling
o Ensure normal operation conditions being restored. Optimal reset point would
be system returned to target operation Temperature.
o Toggle the key switch of main cooling (move key switch out of normal position
and back again)
o Auxiliary cooling should stop now and the red alarm light of main cooling
should switch off
SAB DF System running on auxiliary cooling

o Ensure normal operation conditions being restored. Optimal reset point would
be system returned to target operation Temperature.
o The Error Flag 1506 has to be manually set back with “RS 1506” sent through
the service terminal.
SAB ULT system running on gas backup

o Ensure normal operation conditions being restored. Power and Water for
electric cooling units available.
o Toggle the key switch of aux and main cooling (move key switch out of normal
position and back again)
o Either Main only or both cooling units will operated. When Target operation
Temperature of the Storage is reached toggle the key switch of the main
cooling again.
SAB DF system running on gas backup

o As soon as normal operation conditions are restored the gas backup should
stop operation an electric cooling unit takes over.

16.4 Service operation of backup cooling
In the case it is required to run the backup cooling to maintain the main cooling or
perform modifications on water cooling the backup cooling may be switched on
manually.
CAUTION !
Ensure normal conditions being restored to operate the main cooling
Ask for assistance from a LiCONiC engineer in case of insecurity about
normal conditions and performing the recovery procedure.
Observe cooling after recovery. Contact immediately a LiCONiC engineer
in case of repeated stop of cooling after recovery.
16.4.1 Procedure
STC ULT backup cooling switch to manual operation
o Turn key switch into horizontal position (Manual mode)
o The first stage will switch on immediately. The second stage will kick in after
about 15 minutes (yellow and green light lit)
o When Second stage is operating switch off main cooling
STC ULT restoring normal operation

o Switch back on main cooling. Key switch positioned in in vertical position
o When main cooling is back into normal operation (yellow and green light lit)
switch back key switch of backup cooling into horizontal position.
Contact:
Liconic AG Main: +423 373 63 39
Ralf Kindle (Application/Support) Mobile: +41 79 800 86 42
Cosmas Malin (R&D) Mobile: +41 79 696 95 72
Cornelis Iseli (Cooling expert) Mobile: +41 79 822 56 80

17. Routine Safety Checks
Carefully read all safety instructions before operating the instrument. Be sure that
you fully understand their content. Although the KIWI STORE is intended to be
operated as a remote controlled system, there still are some manual interactions
required for operation. Apart from these operations there are also some very
important safety checks the operator should be familiar with.
The operator should regularly read the display readings and check whether they
match the desired values. Readings like “0.00” or “9.99”, readings that are far
out, instable or that differ over long periods of time could indicate a malfunction of
the instrument or could be caused by faulty settings of the system.
The electrical cables should be routinely inspected for wear, defects or contusion.
There should be no mechanical tension on any cable. Broken connectors should
be replaced immediately. All electrical installations should be kept clean and well
protected from water and humidity.
The same applies to cables and gas tubing. They should be free of mechanical
tension and should show no signs of wear or contusion. Sharp bends should be
avoided under all circumstances. All fittings should be secured. Missing security
nuts must be reinstalled immediately. Any broken pneumatics parts must also be
replaced immediately.
The mechanical appearance of the instrument should be clean and “straight”. All
movements should be smooth and precise. Watch out for unusual noises as
these may indicate misalignments or mechanical wear.
Seals should close the chamber firmly. Check seals by placing a piece of paper
between the seal and door. When trying to pull on the paper, there should be an
even resistance. Check the seal on the glass door, gate and front door.
Condensation, mainly on the front glass door, presents a possible severe danger
as it may lead to water flowing into the electrical parts of the instrument.
Excessive condensation on the glass door will occur when the front door is open
over a long period of time. Furthermore it may also indicate a faulty heater or
high environmental humidity.
Icing on a gassing tube or on a pressurized gas cylinder indicates excessive gas

flow and therefore severe danger. Once icing has been observed leave the room
immediately and call for help. Do not try to locate the source of the problem or try
to fix it. The room may already be contaminated.
WARNING !
Icing on the gas cylinder or other parts of the gassing system indicate
excessive gas flow.
In the case of such icing leave the room immediately and call for help
Do not try to locate the source of the problem or try to fix it

Software
18. Sample Management Software
18.1 Order File

The order file is used to retrieve plates or specific samples from the storage
system. Two types of order files can be used to execute order jobs. The first type
is the plate based export conntaining only 1D of plates to be retrieved. The
second type does only contain the 2D code of the individual sample to be
retrieved.
18.1.1 Plate retrieval

In case of the plate order file, the file is a simple list of 1D barcodes for plate
orders only. These files can be passed onto the system as CSV (Comma
Separated Values) file or as a TXT-File. They can be populated manually by a
user or exported from MS Excel© or a database. Picture 5 below shows an
example of a plate order file.
Picture 5: Sample order file

18.1.2 Sample retrieval
In case of the sample retrieval order file, the file consists of 2D codes. This file
can be passed onto the system as a CSV (Comma Separated Values) file or as a
TXT-File. The file can be populated manually by a user or exported from Excel©
or a database too. Picture 6 below shows an example of a complex order file.
Picture 6: Sample order file

18.2 PlateExplorer
The PlateExplorer is the application to control the SAB storage system. The
application is used for import and export tasks.
The PlateExplorer is a network based application and can run therefore on a

remote computer over the network. Before using the application every user has
to log on. (Picture 7).
Picture 7: The PlateExplorer’s login window
18.2.1 Plate content import

The physical import of plates into the system requires a certain amount of
information being available prior to the storing of the samples. The data for the
samples can be provided in four different ways:
- LIMS integration: A LIMS system does send or provide data of plates going to
be loaded into the system.
- Reading of content: Reading the 1D code and the the 2D codes present on
the plate going to be imported, prior to the import.
- A Combination of above: Is used in cases where it is necessary to confirm the

physical available plate with the preloaded content.
- Specification upon import: The minimum of necessary information can be

provided at the time of import. This functionality is usually used for samples in
container which cannot be read with a 2D reader or where no individual
sample content needs to be stored.
LIMS integration
This is customer specific. In general the data are provided in the background to
the system. The individual plates will be identified with a 1D barcode scanner to
identify which plates are available to be imported at the given time
Scanning of content
With a scanning application the individual plates can be scanned prior to the
import process. The content is read and shown to the user to identify if the read
content does comply with the physical appearance of the plate. Samples
presence, readable and unreadable 2D codes are clearly marked in a visual
display of the plate.
In some cases the scanning has to be performed multiple times with some
cleaning steps in between to get all of the samples correctly identified.

Combination
In some cases the two ways to load content described above have to be
combined. This is namely the case if samples need to be verified upon import. In
this case the content is preloaded to the system but still scanned to confirm the
physical plate does correspond with the expected. If there is a discrepancy
between the physical present to the data the user will be notified. The then
following behavior of the system has to be individually decided.
Definition upon import

If the content of individual plates is not of importance and the plates are only
handled as a whole, the minimal necessary information can be provided up on
the time of import. The most important information is the sample type and the 1D
code. The 1D code can be read with a scanner but the sample type has to be
provided. The sample type is needed to identify the correct storage position
suitable for the particular sample type.
18.2.2 Import
The import of plates is executed within PlateExplorer. Plates which have been
preloaded to the system for being imported can be stored within the system. The
import is triggered by pressing the import button in Plate Explorer:
After pressing the import button the below window is shown. It does display the
preloaded plates waiting for import. The plates are shown on the left.
Picture 8: Import screen 1, preloaded content

The plates to be loaded are selected by ticking the check box. The system will
expect these plates for loading and will not accept any other plate.
Picture 9: Plates selected for import

To confirm the selection of plates the “Find Cassette” button is pressed. The
system will display cassettes suitable for the selected plates. One of the suitable
cassettes is selected.

Picture 10: Cassette selection
After selecting the cassette the view needs to be returned to the Cassette view
tab. This is the representation of the selected cassette. It will display empty and
already occupied levels. The plates for import are moved into an empty position
within the cassette by drag&drop.

Picture 11: Plate set to available free location
With the “Get Cassette” button the placement of the plates is confirmed and the
cassette is presented to the user.
Picture 12: Confirmation of cassette delivery

The plates now are moved manually into the cassette and the loading of the
cassette is confirmed by reading the 1D barcode of the plate. Correctly imported
plates are displayed with a green hook.
Picture 13: Confirmed plate in cassette

After all plates have been successfully loaded to the cassette, the import is
confirmed with a push to the “Confirm Import” button. The cassette is transferred
back to the storage
18.2.3 Export
The export of plates and samples is executed within PlateExplorer. Plates which
have samples on them or are requested as a whole can be exported from the
system. The export is triggered by pressing the export button in Plate Explorer:
After pressing the export button the export is started. Depending on the
integration the plate overview is immediately displayed or the job selection is
opened. In the job selection the job is searched on the computer and sent to the
system. After this also the plate overview is shown.

Picture 14: Selecting a job file
The job overview does display the involved cassettes and plates/samples.

Picture 15. Job Overview
By selecting the individual cassette or the individual the respective location of the
requested sample or plate is displayed.

Picture 16: Display of requested sample
By pushing the button “Get Cassette” the relevant cassette is presented to the
user. With the 1D hand scanner the correct palate is confirmed. If whole plate is
exported this is removed from the system. Are only individual samples are
requested, the sample is removed from the plate and the plate returned to the
cassette. After the export of plates and samples has been finished the export is
finished by pushing the button “Confirm Export”. The plates are removed from the
cassette and the cassette is returned to the storage.

Picture 17: Display of correct plates
Picture 18: Plates removed from system

18.2.4 Manual Operations
In this chapter the functionality accessible to the administrator and the normal
user are described. There is only one difference between user and administrator.
Whilst the user has only access to the assigned area, the administrator has
access to the full store. Therefore the difference will not be mentioned in this part
of the manual anymore.
Get / Set Cassette

The Get and Set Cassette functionality of the PlateExplorer is used to retrieve
and return cassettes into the store manually. To access the commands select a
cassette with a left click and perform a right click. A context menu with the Get
Cassette and the Set Cassette commands are presented.
Picture 19: Cassette context menu

18.2.5 Layout of the PlateExplorer
The main window of the PlateExplorer is subdivided into the Menu bar, a quick
usage button bar, the contents tree on the left and the detailed information view
on the right (see Picture 20). The detailed view on the right can be switched from
a “Detail” view showing information of Partition/Cassette/Plate to a more
graphical expression “View” (see Picture 21)
Picture 20: Main screen detailed view of the PlateExplorer

Picture 21: Main screen graphical view of PlateExplorer

Safety Sheets
Safety guides for the work in the service area
of the LiCONiC SAB-series
(Ralf Kindle, 27.01.2016 REV 1)
Work on this device should only be performed by Liconic Service

Engineers or Service Engineers Certified by Liconic to work on SAB
systems.
Works, that require the regulations:
maintenance(repair, maintenance, inspection), cleaning, hardware changes, disturbance
removal
Behavior on the machine, that requires the regulations:

lean into the service area, get into / out of the service area, detention at the service area
Work on the device should only start following all given

regulations in this document!
Potential danger Protection mechanism
Contact with dangerous samples • Additional to the usage of thermal

protection clothing, please use Nitrile-
gloves or gloves with comparable
safety properties concerning
dangerous materials
• Check skin compatibility towards the
used disposables
Lacking air condition / lack of oxygen • Due to very specific local rules and
(Measurement value < 20,9%) / too high regulations in different countries it is
carbon dioxide levels the customers / owners responsibility
to install suitable oxygen (O2) and
carbon dioxide (CO2) monitor systems
which trigger alarms to protect the
operators around the SAB store.
• For works within the area, a breathing
mask is recommended (no Cartridge-
mask, as this does not help).
• Personal oxygen monitors must be
worn when entering the service area.
Low temperature skin burn injury / freezing • Only work in thermal protection
clothing (thermally protect body, head
and hands). Do not touch or pick up
anything within the -80°C area without
the use of suitable equipment. Never
lean into the -80°C area under the
isolation or step into it. Generally the
work in the service area should be
limited to 15 minutes time.

Pressure by mechanical parts • Before entering the service area,
switch off the device and put a sign,
saying „Do not switch on device!“ on
it. After the work a reset and re-
initialization is necessary. Attention:
Neither the cooling nor the handling
should be switched off longer than 30
minutes!
Physical stress due to narrowness of the • Duration of the work within the area
service area. should be limited to 15 minutes.
Bodily harm by sharp edges or corners (e.g. • Overtired or for different reasons
air condition pipes) unconcentrated persons should not
do maintenance work.
Suffocation / burning by indirect contact with • When LN2 has been introduced into
LN2 the system and the DEWAR needs to
be exchanged, wear protective
clothing.
• In case no exhaust leading the N2
gas out of the room, only enter the
room wearing a breathing mask with
oxygen supply.
• During operation of LN2 backup, do
not touch any piping showing frost
without protective clothing.
Bodily harm while entering the area by • Use suitable equipment for access to
slipping off or falling down service area (save and robust ladder)
Contaminated or destroyed samples or • To avoid a contamination of or by the
probes by the user (product safety and samples, no sharp edge tools should
personnel safety) be used within the service area.
Sample location / Database integrity • By using barcode readers / scanners
and guiding the SAB user Liconic AG
is doing utmost to make sure that real
sample location matches the stores
database but due to the semi -
automatic nature of the SAB store the
user / customer is finally responsible
for sample location by moving them
into a particular cassette and
associated cassette level manually.
General Rules:
1) DO NEVER WORK ALONE WITHIN THE DEVICE. ALWAYS ORGANIZE A
SECOND PERSON AS SAFTEY GUARD ON THE OUTSIDE OF THE DEVICE
THAT IS CONNECTED TO YOU VIA RADIO. FURTHER AN ADMINISTRATIVE
PERSON MUST BE REACHABLE IN A SHORT TIME. BOTH MUST BE 100%
PHYSICALLY FIT.
2) WHILE WORKING IN THE SERVICE AREA, ALWAYS SWITCH ON THE

INTEGRATED LAMP. NEVER WORK IN THE DARK.
3) FOR ALL KINDS OF WORK, CARRY AN OXYGEN MEASUREMENT DEVICE WITH

YOU, HAVING A DISPLAY FOR THE ACTUAL VALUE, AN ADJUSTABLE VALUE
FOR THE LIMIT AND AN OPTICAL/ACCOUSTIC SIGNAL WHEN THIS LIMIT IS
REACHED.

4) ENTERING OR WORKING WITHIN THE -80°C AREA OF THE DEVICE IS
ALLOWED TO TRAINED LiCONiC PERSONNEL ONLY. THE USER SHOULD NOT
WORK WITHIN THIS AREA OR EVEN ENTER IT. OBJECTS FALLEN DOWN OR
THINGS TO BE ALIGNED WITHIN THIS AREA SHOULD ONLY BE PICKED UP OR
MOVED WITH THE HELP OF PROPER TOOLS.
5) IF THERE ARE DOUBTS ABOUT THE FUNCTIONALITY OF THE DEVICE

(FATIGUE, ABRASION OR BREAKING OF PARTS), THE USER SHOULD
IMMEDIATELY REQUEST SERVICE FROM LiCONiC AND THE USER SHOULD
STOP USING THE SYSTEM IMMEDIATELY UNTIL ALL REPAIRED.
6) IN CASE OF A FIRE THE DEVICE MUST NOT BE ENTERED UNDER ALL

CIRCUMSTANCES.
7) EVERY PERSON TAKING PART IN THE WORK ON THE DEVICE SHOULD BE

INFORMED ABOUT THE SAFETY REGULATIONS BEFORE STARTING TO
WORK. ONCE A YEAR AN ACTIVITY PLANNING SHOULD BE MADE WITH ALL
PARTICIPATING PERSONS.

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Operating Manual

The Information contained in this document is subject to change without notice.

Information provided by LiCONiC AG is believed to be accurate and reliable. However, the

Telephone: +423 373 63 39

Installation and Servicing

StoreX Series V001 2 RKi, 05.02.2016

StoreX Series V001 3 RKi, 05.02.2016

StoreX Series V001 4 RKi, 05.02.2016

StoreX Series V001 5 RKi, 05.02.2016

1.1 Intended Use

1.2 Unintended Use

Read the safety instructions in this Manual carefully.

1.3 Product Identification

1.4 Scope of Delivery

Exact delivery scope may differ according to project scope

StoreX Series V001 6 RKi, 05.02.2016

Authorized Engineer Authorized person responsible for installation, initial start

Plate Any SBS-Format sample plate or tube rack.

Cryo Box Storage card board box for cryo tubes

Samples Any individual container containing sample material,

1.7 Target User Group

Instrument operation requires a trained user.

1.8 Importance of the Safety Instructions

StoreX Series V001 7 RKi, 05.02.2016

Indicates the possibility of severe personal injury, loss of life or equipment

Indicates the possibility of severe equipment damage if instructions are not

Gives helpful information about the equipment.

May be harmful to your eyes.

1.10 Safety - Basics

1.10.2 Mechanical Hazards

1.10.3 Electrical Hazards

1.10.4 Chemical, Biological and Radioactive Hazards

StoreX Series V001 8 RKi, 05.02.2016

1.10.5 Extreme Temperature Hazards

1.10.6 Suffocation Hazard

1.11 General Safety Regulations

In Europe, the System Integrator must provide a Conformity Declaration upon

StoreX Series V001 9 RKi, 05.02.2016

1.11.3 Spare Parts to be used

StoreX Series V001 10 RKi, 05.02.2016

The modular mechanical design in combination with extremely simple commands

2.2 Kiwi Store SAB Features

Front User Door for

StoreX Series V001 11 RKi, 05.02.2016

2.3 Kiwi Store Configuration and Capacity Overview

Capacity, given in Cassettes

StoreX Series V001 12 RKi, 05.02.2016

StoreX Series V001 13 RKi, 05.02.2016

3.1 Kiwi Store SAB ULT

The modular mechanical design in combination with extremely simple commands

Front User Door for

StoreX Series V001 14 RKi, 05.02.2016

Name Temperature Humidity

StoreX Series V001 15 RKi, 05.02.2016

5. Backup solutions for Kiwi Stores SAB

Name Temperature Principle

StoreX Series V001 16 RKi, 05.02.2016

5.2.1 Auxiliary Refrigeration Kiwi Store SAB DF

To provide the complete redundancy a second evaporator is installed inside of