Endocrine Experiment 2

Name: Odezza Maon, Arianne Manuel, Ivy Mendoza, Katherine Nolasco, Stephanie Ong, Anhiro Rodriguez,
Marcus Sahagun, Rome Taguic

Exercise 4: Endocrine System Physiology: Activity 2: Plasma Glucose, Insulin, and Diabetes Mellitus Lab Report
Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.
1. Which of the following statements is false?

You correctly answered: d. Insulin is a hormone secreted into the stomach to aid with starch digestion.
2. Which of the following statements is true?

You correctly answered: d. All of these statements are true.
3. A diagnosis of type 1 diabetes mellitus implies that

You correctly answered: b. the pancreas is not producing sufficient insulin.
4. A diagnosis of type 2 diabetes mellitus implies that

You correctly answered: c. the cells of the body are unresponsive to circulating insulin.
5. Glucagon is a hormone
You correctly answered: b. that opposes the action of insulin.
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Experiment Results
Predict Question:
Predict Question: To measure the amount of plasma glucose in a patient sample
Your answer : b. the optical density of the sample will be measured and the glucose concentration will be extrapolated from
the glucose standard curve.
Stop & Think Questions:

Why does the color in the tubes vary?
You correctly answered: c. The tubes contain different amounts of glucose.
Experiment Data:
Tube Optical Density Glucose (mg/deciliter)

Part 1 1 0.30 30
2 0.50 60
3 0.60 90
4 0.80 120
5 1.00 150
Part 2 1 0.73 105
2 0.79 115
3 0.89 131
4 0.83 120
5 0.96 144
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Post-lab Quiz Results
You scored 100% by answering 6 out of 6 questions correctly.
1. A male patient has had successive fasting plasma glucose readings of 115, 110, and 122 mg/dl. The healthcare provider
will inform him that
You correctly answered: c. he appears to have impairment or borderline impairment of insulin-mediated glucose uptake by
his cells.
2. To obtain an accurate spectrophotometric measurement of the glucose concentration in the sample

You correctly answered: d. heparin is added to prevent blood clots.
3. In the spectrophotometric assay used in this experiment, the __________ as the glucose concentration in the sample
increases.
You correctly answered: b. optical density increases
4. A female patient has had successive fasting plasma glucose readings of 130, 140, and 128 mg/dl. The healthcare
provider will inform her that
You correctly answered: a. she has developed diabetes.
5. To maintain plasma glucose homeostasis

You correctly answered: d. insulin-mediated transport of glucose into cells acts as negative feedback when plasma glucose
levels rise.
6. A laboratory technician withdraws a blood sample from a vein in your upper arm knowing that
You correctly answered: b. the plasma glucose concentration will be the same in both the arm vein and the index finger.
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Review Sheet Results
1. What is a glucose standard curve, and why did you need to obtain one for this experiment? Did you correctly predict
how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve?
Your answer:
Glucose standard curve is essential to determine the corresponding amount of glucose for a specific optical density. It is
used to determine the amount of glucose present in each sample. Yes we are able to predict plasma glucose of patients
based on the standard glucose curve.
2. Which patient(s) had glucose reading(s) in the diabetic range? Can you say with certainty whether each of these
patients has type 1 or type 2 diabetes? Why or why not?
Your answer:
Patient 3,4, and 5 are diabetic. We cannot say with certainty if they have type 1 or type 2 diabetes based on this result
alone.
3. Describe the diagnosis for patient 3, who was also pregnant at the time of this assay.
Your answer:
The patient has a gestational diabetes. Blood sugar level usually go back to normal after pregnancy.
4. Which patient(s) had normal glucose reading(s)?

Your answer:
Patient 1 had a normal glucose reading.
5. What are some lifestyle choices these patients with normal plasma glucose readings might recommend to the borderline
impaired patients?
Your answer:
Proper diet and exercise are recommended.
03/28/23 page 4

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Name: Odezza Maon, Arianne Manuel, Ivy Mendoza, Katherine Nolasco, Stephanie Ong, Anhiro Rodriguez,

Marcus Sahagun, Rome Taguic

Pre-lab Quiz Results

1. Which of the following statements is false?

2. Which of the following statements is true?

3. A diagnosis of type 1 diabetes mellitus implies that

4. A diagnosis of type 2 diabetes mellitus implies that

Stop & Think Questions:

Tube Optical Density Glucose (mg/deciliter)

2. To obtain an accurate spectrophotometric measurement of the glucose concentration in the sample

5. To maintain plasma glucose homeostasis

4. Which patient(s) had normal glucose reading(s)?

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