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    Pure Culture

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    MOHAMAD ALFITROH HARUN
    Bacterial isolation involves removing microbes from their natural environment and growing them as pure cultures in artificial mediums using aseptic techniques to prevent contamination. There are several methods for inoculating bacteria onto different media, such as using an ose needle to puncture upright media or an ose loop to scratch tilted media. After inoculation, the media is incubated at specific temperatures to provide suitable growth conditions for the bacteria. The goal of bacterial isolation is to obtain a pure strain of the target microorganism by diluting and transferring cultures through multiple stages using selective media, with the final isolate being re-isolated to confirm purity. Important considerations for isolation include the microbial properties, origin, suitable growth medium, and methods for planting, incub

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    Pure Culture

    Uploaded by

    MOHAMAD ALFITROH HARUN
    20 views7 pages
    Bacterial isolation involves removing microbes from their natural environment and growing them as pure cultures in artificial mediums using aseptic techniques to prevent contamination. There are several methods for inoculating bacteria onto different media, such as using an ose needle to puncture upright media or an ose loop to scratch tilted media. After inoculation, the media is incubated at specific temperatures to provide suitable growth conditions for the bacteria. The goal of bacterial isolation is to obtain a pure strain of the target microorganism by diluting and transferring cultures through multiple stages using selective media, with the final isolate being re-isolated to confirm purity. Important considerations for isolation include the microbial properties, origin, suitable growth medium, and methods for planting, incub

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    pure culture

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    Bacterial isolation involves removing microbes from their natural environment and growing them as pure cultures in artificial mediums using aseptic techniques to prevent contamination. There are several methods for inoculating bacteria onto different media, such as using an ose needle to puncture upright media or an ose loop to scratch tilted media. After inoculation, the media is incubated at specific temperatures to provide suitable growth conditions for the bacteria. The goal of bacterial isolation is to obtain a pure strain of the target microorganism by diluting and transferring cultures through multiple stages using selective media, with the final isolate being re-isolated to confirm purity. Important considerations for isolation include the microbial properties, origin, suitable growth medium, and methods for planting, incub

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    © All Rights Reserved
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    20 views7 pages

    Pure Culture

    Uploaded by

    MOHAMAD ALFITROH HARUN
    Bacterial isolation involves removing microbes from their natural environment and growing them as pure cultures in artificial mediums using aseptic techniques to prevent contamination. There are several methods for inoculating bacteria onto different media, such as using an ose needle to puncture upright media or an ose loop to scratch tilted media. After inoculation, the media is incubated at specific temperatures to provide suitable growth conditions for the bacteria. The goal of bacterial isolation is to obtain a pure strain of the target microorganism by diluting and transferring cultures through multiple stages using selective media, with the final isolate being re-isolated to confirm purity. Important considerations for isolation include the microbial properties, origin, suitable growth medium, and methods for planting, incub

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    Pure Culture

    mohamad alfitroh harun


    Pure culture/Isolation
    of bacteria
    Bacterial isolation is the taking or removal of microbes from their environment
    in nature and growing them as pure cultures in artificial mediums. Bacteria
    transferred from place to place must use aseptic procedures. Aseptic means
    free from sepsis is a condition contaminated due to other microorganisms.
    Aseptic techniques are especially important when working in contact with
    bacteria. Some of the tools used to carry out this procedure are bunsen and
    laminarair flow. If not executed properly, there is a possibility of contamination
    by other myoorganisms that will interfere with the expected results. Aseptic
    techniques also protect laboratories from bacterial contamination (Singleton
    and Sainsbury, 2006).
    Bacterial
    isolation methods
    There are several methods for inoculating bacteria according to the type of medium
    for which they are intended. In the medium to be upright, a puncture method is
    carried out using an ose needle. On the medium to be tilted, a scratch method is
    carried out using an ose loop. In petridish medium, streak plate method, pour plate
    or spread plate method can be used. After inoculation, an incubation process is
    carried out, which is storing the medium in a tool or container with a certain
    temperature and period certainly, thus creating an environment that provides
    suitable conditions for bacterial growth (Harley and Presscot, 2002). The
    methods for isolating pure cultures of microorganisms according to Pelczar and
    Chan (1986) are: - scratch cup, - stocking cup, -pour cup.
    Principles and
    Purpose of Insulation

    Isolation of a pure strain can be carried out in principle in stages. The first level is free to do manually
    by diluting it as far as possible, often to 10-4 or 10-6.The second level is by media that is selective for
    certain microbes or certain microbes that may still be in the same group. The third level of the colony,
    which seems to be possible, still needs to be re-diluted or re-isolated in order to be more convincing.
    proof that the isolate strains obtained are really pure strains (Mulyono, 1992).The working principle of
    bacterial isolation is quite simple, namely by inoculating a small number of bacteria in a certain
    medium that can constrain bacterial life.
    The purpose of culture transfer is to master the technique of transferring bacterial cultures from one
    container to another aseptically, so that only the expected pure culture grows. This is especially
    important in the early stages of microbial isolation work, especially those derived from culture stocks
    (not from substrates).
    Samples needed for
    isolation
    Before isolating, sampling is first carried out. Various sampling according to the place:
    1. Soil sample
    If the desired microorganism is in the soil, then the way of taking it is adapted to the
    purpose. For example, if what is desired is rhizosphere microorganisms, then samples are
    taken from around the roots near the soil surface.
    2. Water sample
    The retrieval technique is simply to open the lid of the petri dish containing sterile media
    for ±5 minutes.

    According to Jutono (1980), there are several ways to isolate


    microbes. For this isolation, several important things must be
    considered, including:
    1. The properties of the microbial species to be isolated
    2. Place of life or origin of the microbes
    3. Medium for its suitable growth
    4. How to plant such microbes
    5. How to incubate these microbes
    6. How to test that the isolated microbes have been pure cultures
    and are in accordance with what is intended
    THANK YOU...

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