Service NEW Manual 2010 daVINCI Quattro

VERSION 6.
10a
SERVICE MANUAL
Complement to DYNEX service manual
This manual is intended for bioMérieux staff only.

4501 - 2053 - C - en - 2010/04 — P/N 4501 - 2053
bioMérieux S.A. 69280 Marcy l'Etoile / France

RCS Lyon 673 620 399
Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90
http://www.biomerieux.com
REVISIONS
The list of revisions below summarizes replacements or additional pages in your

DAVINCI® Quattro™ Service Manual.
Date of Page(s)
Version Modification
printing modified
A (6.02) 2006/05 Creation – BTL 0328222 All
B (6.08) 2008/10 Update All
C (6.10a) 2010/04 Update related to software version 6.10a All
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DAVINCI Quattro™ Service Manual Revisions - 1
TABLE OF CONTENTS
1 System Composition
1.1 DAVINCI QUATTRO (art. code 200408) ..............................................................................................1-2
1.2 Sample Racks for long or short tubes...................................................................................................1-7
1.3 Control bottles ......................................................................................................................................1-7
1.4 Caps for Control bottles ........................................................................................................................1-8
1.5 Reagent bottles ....................................................................................................................................1-8
1.6 Deep well plates ...................................................................................................................................1-8
1.7 Deep weel dilution strips.......................................................................................................................1-9
1.8 Sample tips...........................................................................................................................................1-9
1.9 Reagent tips .......................................................................................................................................1-10
1.10 Strip plates (Break-4)..........................................................................................................................1-10
2 Instrument Installation
2.1 Prequisites............................................................................................................................................2-1
2.2 Physical installation of the instrument...................................................................................................2-3
2.3 Instrument Check / Setup .....................................................................................................................2-8
2.3.1 Check / Setup the Date & Time and Time properties and the printer page size .....................2-8
2.3.2 Startup the instrument & Print the Self Test............................................................................2-9
2.3.3 Language selection (optional).................................................................................................2-9
2.3.4 Print the workspace calibration data and the module coordinates..........................................2-9
2.3.5 User administration (optional)...............................................................................................2-10
2.3.6 Setup Laboratory Information to appear at the top of the result reports ...............................2-11
2.3.7 Setup the Sample Identification Module ...............................................................................2-11
2.3.8 Install the assay protocols (depending on the customer needs) ...........................................2-13
2.3.9 Setup the correct sample tubes and caddies........................................................................2-13
2.4 Mechanical tests .................................................................................................................................2-16
2.4.1 Set the deepwell dilution plate or deepwell dilution strips .....................................................2-16
2.4.2 Run the Tip Alignment Test ..................................................................................................2-17
2.4.3 Run the Sample Rack Pickup & Vertical Barcode Scanner Test ..........................................2-19
2.4.4 Run the Plate Movement Test ..............................................................................................2-20
2.4.5 Run the Horizontal Barcode Scanner Test ...........................................................................2-22
2.5 Instrument qualification.......................................................................................................................2-23
2.5.1 Dprog manual entry ..............................................................................................................2-23
2.5.2 Reader Verification ...............................................................................................................2-23
2.5.3 Calibration plate....................................................................................................................2-32
2.5.4 Pipette volume calibration.....................................................................................................2-34
2.5.5 High volume Air Gap check ..................................................................................................2-45
2.5.6 Pipettor Verification ..............................................................................................................2-45
2.5.7 Wash head offsets ................................................................................................................2-50
2.5.8 Washer Pump calibration A, B, C, D.....................................................................................2-58
2.5.9 Washer Verification ..............................................................................................................2-66
2.5.10 Incubator verification ............................................................................................................2-70
2.5.11 Qualification results ..............................................................................................................2-78
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DAVINCI Quattro™ Service Manual I-1
Table of Contents
2.6 Burn-in ................................................................................................................................................2-78

2.7 ESP setup...........................................................................................................................................2-80
2.8 LIS-LINK configuration set up (optional) .............................................................................................2-85
2.9 Enabling the LIS communication in THE DAVINCI QUATTRO software (optional).............................2-91
3 Software Items
3.1 Windows XP Logon ..............................................................................................................................3-1
3.2 davinci quattro LOG ON .......................................................................................................................3-1
3.3 File structure.........................................................................................................................................3-2
3.4 Database ..............................................................................................................................................3-4
3.5 Dead volumes table ............................................................................................................................3-25
3.6 Application software - software menus ...............................................................................................3-26
3.7 Cleaning up the hard disk ...................................................................................................................3-31
3.8 Backup................................................................................................................................................3-31
3.9 Log File...............................................................................................................................................3-31
3.10 Auto Error Recovery ...........................................................................................................................3-34
3.11 Engineering mode...............................................................................................................................3-37
3.12 Some other functions..........................................................................................................................3-39
3.12.1 Data editor ............................................................................................................................3-39
3.12.2 Setup the Timeline Settings..................................................................................................3-41
3.12.3 Create shortcut buttons ........................................................................................................3-43
4 Start Up & SelF Test
5 Manual Control
6 Hardware & Troubleshooting & Disassembling

6.1 Incubator...............................................................................................................................................6-2
6.2 Reader..................................................................................................................................................6-5
6.3 Washer ...............................................................................................................................................6-13
6.4 Drawer ................................................................................................................................................6-25
6.5 Barcode Scanner Module (SID module) .............................................................................................6-27
6.6 EPOD .................................................................................................................................................6-28
6.7 Pipette Module....................................................................................................................................6-33
6.8 Removal of the system covers............................................................................................................6-38
6.8.1 Top Cover Assembly ............................................................................................................6-38
6.8.2 Rear Cover Assembly...........................................................................................................6-38
6.8.3 Blue Cover Assembly ...........................................................................................................6-39
6.8.4 Right side panel....................................................................................................................6-40
6.9 XYZ drive............................................................................................................................................6-41
6.10 Other parts..........................................................................................................................................6-57
7 Calibration & Verification

7.1 Pipettor .................................................................................................................................................7-1
7.1.1 Pipette collar calibration..........................................................................................................7-1
7.1.2 Calibration of the E sensor positions ......................................................................................7-2
7.1.3 Workspace calibration ............................................................................................................7-3
7.1.4 Drawer Calibration ..................................................................................................................7-8
7.1.5 Module calibration ..................................................................................................................7-9
7.2 Washer ...............................................................................................................................................7-14
7.2.1 Calibration of the Vacuum Sensor ........................................................................................7-14
7.2.2 Calibration of the Dispense Pump Pressure .........................................................................7-15
7.3 Barcode Scanner ................................................................................................................................7-18
7.3.1 Selection of the barcode label type.......................................................................................7-18
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I-2 DAVINCI Quattro™ Service Manual
Table of Contents
7.3.2 Verification of the sample barcode scanner..........................................................................7-20

7.3.3 Verification plate barcode scanner alignment .......................................................................7-20
7.3.4 Adjustment of the sample / plate barcode scanner...............................................................7-21
8 ESP (Electronic Signature Pipetting)

8.1 ESP description ....................................................................................................................................8-1
8.2 Software limitations...............................................................................................................................8-5
9 Firmware Upgrade
9.1 Installation of Flash Program ................................................................................................................9-1
9.2 Procedure to flash the firmware ............................................................................................................9-2
9.2.1 Flash the Firmware of the Communication (EPOD) or Pipette modules. ................................9-2
9.2.2 Flash the Firmware of the Incubator modules.........................................................................9-2
9.2.3 Flash the Firmware of the Drawer / SID, Reader, Washer or XY board modules. ..................9-3
9.3 Firmware troubleshooting .....................................................................................................................9-5
10 Preventive Maintenance
10.1 Operator maintenance ........................................................................................................................10-1
10.2 Service Preventive maintenance ........................................................................................................10-2
11 Spare parts list

11.1 Spare Parts.........................................................................................................................................11-1
11.2 Tools...................................................................................................................................................11-3
11.3 Accessories ........................................................................................................................................11-4
11.4 Others.................................................................................................................................................11-4
12 Repair & Cleaning & Decontamination Procedure

12.1 Repair procedure for instrument modules...........................................................................................12-1
12.2 Cleaning .............................................................................................................................................12-2
12.3 Decontamination.................................................................................................................................12-2
13 Running an assay with a LIS connection

13.1 Principle..............................................................................................................................................13-1
13.2 Running an assay with a LIS connection ............................................................................................13-2
14 Known problems / resolution
15 Addendum: Pipetting qualification
16 Appendix: Installation of the IVT Logger Software
NOTE: Screenshots, figures, and messages are given for information

purposes only.
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DAVINCI Quattro™ Service Manual I-3
1 SYSTEM COMPOSITION
DAVINCI QUATTRO Computer +

200408 Keyboard +Mouse Printer Monitor
7 X Sample Racks, 100 mm tubes (1 pcs) 7 X Sample Racks, 75/80 mm tubes (1 pcs)
200414 OR 200415
Control Bottles (344 pcs) Caps, Control bottles (2000 pcs)

272240 272241
Reagent Bottles (24 pcs) Reagent tips (4 x 108 pcs)

200413 200411
Sample tips (4 x 108 pcs) Strip plates (100 pcs)

200410 278303
Diagnotic Kit Application Notes User CD

280103 200426
Deepwell dilution plates (10 pcs) Deepwell dilution strips (250 pcs) (optional)
200412 (optional) OR 200429
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DAVINCI QUATTRO™ Service Manual 1-1
System Composition
DAVINCI QUATTRO (art. code 200408)
1.1 DAVINCI QUATTRO (ART. CODE 200408)
The complete list is available on the GCS portal.
DAVINCI QUATTRO Unit (reader with 405, 450, 620 and 690 nm filters)
RS 232 cable, PC - QUATTRO
Cleaning Wire, dispense needles , 0.018” , 2 pcs (art. code 45688572)
Cleaning Wire, aspirate needles , 0.040” , 2 pcs (art. code 45688571)
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1-2 DAVINCI Quattro™ Service Manual
System Composition
Deepwell dilution strip Holder, 2 pcs (art. Code 7513093643)
Deepwell dilution plate holder, 2 pcs (art. Code 7513060542)
Control Rack (art. code 45688578)
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DAVINCI Quattro™ Service Manual 1-3
System Composition
Reagent rack (art. code 7513060541)
Tip funnel (art. Code 7513060543)
Caddy, sample racks (art. code 45688575)
Plastic Tips, sample, set of 4 x 108 (art. code 200410)
Plastic Tips, reagent, set of 4 x 108 (art. code 200411)
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System Composition
Reagent bottle with cap, set of 24 (art. code 200413)
Purge trays, 5 pcs (art. code 45688570)
Plate carrier, 4 pcs (art. code 45688577)
Tray, washer module (dynex code 13001790)
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System Composition
Head assembly, 8 way (art. code 45688526)
Power Cord, 220 V , European (dynex code 575100600)
Hex Driver, 4 mm (dynex code 42000360)
Key, door (art. code 45688576)
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System Composition
Sample Racks for long or short tubes
1.2 SAMPLE RACKS FOR LONG OR SHORT TUBES
Sample Rack, 75 mm tubes, 14 positions , 7 pcs (art. code 200415)
Rack with spacer

Tube length until 80 mm
Sample Rack, 100 mm tubes, 14 positions , 7 pcs (art. code

200414)
Rack without spacer
1.3 CONTROL BOTTLES
Control bottle, set of 344 (art. code 272240)
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System Composition
Caps for Control bottles
1.4 CAPS FOR CONTROL BOTTLES
Cap, Control bottle, set of 2000 (art. code 272241)
1.5 REAGENT BOTTLES
Reagent bottle with cap, set of 24 (art. code 200413)
1.6 DEEP WELL PLATES
Deep well plate, 10 pcs (art. code 200412)
Used in combination with a Base Plate, deep well plate (article code 7513060542).
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System Composition
Deep weel dilution strips
1.7 DEEP WEEL DILUTION STRIPS
Deep well dilution strips, 250 pcs (art. code 200429)
Used in combination with the deepwell dilution strip holder (article code
7513093643).
1.8 SAMPLE TIPS
Plastic Tips, sample, set of 4 x 108 (art. code 200410)
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System Composition
Reagent tips
1.9 REAGENT TIPS
Plastic Tips, reagent, set of 4 x 108 (art. code 200411)
1.10 STRIP PLATES (BREAK-4)
Strip plates, set of 100 (art. code 278303)
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2 I N S T R U M E N T I N S TA L L AT I O N
2.1 PREQUISITES
• Tool Set containing :

− Screwdrivers
− Wrench nº 13, 14, 16, 24
− Bubble level
− Weighing scale or volume measurement cylinders (10 ml & 50 ml)
• Needed tools:
bMx code Description

45688570 LOW-SIDED VESSEL (50 ml)
45688563 CALIBRATION PLATE, MODULE
45688564 COLLAR ALIGNMENT PLATE CARRIER
280103 DIAGNOSTIC KIT (LIQUIDS)
45688569 FIXTURE, WASHER ALIGNMENT
45688567 FIXTURE, WORKSPACE CALIBRATION
284119 INCUBATOR VERIFICATION TOOL
45688568 READER VERIFICATION PLATE
278303 STRIP PLATES (BREAK 4) (100 PCS)
NOTE: Always check the bMx code on the GCS portal to use the correct
products.
• To calculate the verification results, the engineer needs a computer

equipped with MS excel 2000 or higher.
• The Dprog Excel file is necessary for the instrument qualification (available
from the GCS Portal).
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Instrument Installation
Prequisites
• Check that the firmware versions are the following or higher:
Firmware Version
Communication module CM 1.02
Absorbance reader AM 1.21
Drawer module DM 2.20
Washer module WM 1.15
Incubator module IM 2.09
Pipette module PM 1.05
XY module XY 1.02
Sample ID module SM 2.20
− If necessary, update the firmware.
CAUTION! After any firmware update, run the self test again and make a
new printout.
The DAVINCI QUATTRO is shipped in a wooden crate (1300 W x 1130 D x 1090 H).
It’s weight will be about 200 kg. The crate is palleted. A fork lift or some form of
pallet jack is required to move the crate.
9/16” bolts
The following steps should be followed and appropriate notes and printouts have to
be attached to the checklist as evidence that the procedures have been followed
during installation. A copy of this record can be made and left with the instrument
after installation.
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Physical installation of the instrument
2.2 PHYSICAL INSTALLATION OF THE

INSTRUMENT
1. Have 4 people ready to lift the DAVINCI QUATTRO.

2. If needed, have a cart ready to transport the instrument from the uncrating
area to the laboratory. It must be capable of carrying 125 kg.
3. Using a wrench (nº 14 - 9/16”), remove the five bolts from around the base of
the crate.
4. At least two people, using the handles in the crate, should raise the crate top
straight up to clear the instrument. Set the crate top to the side.
5. Using a wrench (nº 13 - 1/2”), remove the two bolts from the Removable
Front Support Assembly. Set the Removable Front Support Assembly to the
side.
½” bolts
6. The instrument is ready to be lifted onto the cart or the workbench. Do not
pick up the unit by the outside skins/cover. Hand support the unit from
bottom frame. Slide the instrument out the front side of the crate. Do not pick
unit straight up, it will damage the support legs. Once the unit is out of the
back support area then the unit can be picked up.
7. When the instrument has been installed onto the workbench, remove the
transport protections. Remove the plastic bag from the instrument. Inspect
for any visible damage.
8. Open the blue cover slowly. Pull the pink foam on left side straight out.
9. Remove the accessory box on right side by tilting forward and sliding box
out.
10. Remove the arm pink support and box bottom support.
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11. Remove the pink arm support in the washer module.
12. Remove the long white cover support from the front.
13. Using a bubble level, ensure that the system is level on the bench. If
necessary, level can be achieved by loosening the nut (wrench Nº 24) at the
top of the screwed-in legs and turning the legs (wrench nº 16) to make them
shorter or longer. After adjusting the legs, tighten the nuts again.
14. Verify that all modules are in place and secured.
15. Open the accessory box and check the contents (see Sales Package List).
16. Insert the washer module plate carrier into the washer module by securing
the two clamps on the bottom to the two railings that protrude from the rear
of the washer cavity. Try not to bend the railings or stress them too much. It
may be necessary to move the lever arm to the eject position to seat the
plate carrier on the railings.
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17. Fit the dispense and aspirate tubing to the wash head. It is easier to attach
the small diameter dispense tubing first, then the larger aspirate tubing.
Mount the washer head onto the two pins on the carrier-arm in the washer.
The wash head is leveled with the screw on the top.
18. Secure the tubing in the associated clips so that they are out of the way and
don’t drag across the tops of plates. Ensure the smaller diameter tubing is
properly inserted into the pinch valve.
19. Rinse the wash buffer bottles with DI water and then fill them about halfway
with DI water. Tighten the lids. Handle the wash buffer bottles carefully.
20. Slide the wash buffer bottles into their slots, fasten the quick-disconnects,
plug in the electrical connections and insert the tubing into the pinch valve.
21. Connect the two waste bottle-tubing fittings. Ensure that the cap is tight.
Be aware that the

edges of these black
tubing holders are
very sharp.
You may remove black

parts.
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22. Install all the accessories in the workspace; sample racks, reagent tray,
control rack, funnel, plastic tips, deep well plates, tips, ….
23. Install the computer, monitor, mouse and printer.

24. Connect the RS-232 cable from the instrument to COM 1 on the computer.
25. Important ! Check for the correct fuse rate:
220 V - 240 V : slow blow 3.2 A - 250 V (5 x 20 mm)
100 V - 120 V : slow blow 6.3 A - 250 V (5 x 20 mm)
Note: The 6.3 A fuse is not included in

the Sales Package.
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26. Plug the power cord into the instrument and into the standard outlet.
NOTE: In case a UPS is necessary, it is recommended to use a 1500 VA

system + computer.
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Instrument Check / Setup
2.3 INSTRUMENT CHECK / SETUP
2.3.1 Check / Setup the Date & Time and Time properties
and the printer page size
1. Set the correct Date & Time and Time Zone in Windows XP
The computer is delivered with the Time Zone GMT + 01:00.
• Switch on the computer.
• Log as "super" administrator for Windows.
• Select Start - Settings - Control Panel - Date & Time to make any
changes.
2. Set the correct Printer Page Size (default is "letter")

Ensure the printer is switched on. From the Windows desktop screen, select Start -
Settings - Printer and Faxes. Select the printer and properties. Select the “Printing
Preferences” button.
e.g. you can change “letter” by “A4”. If the wrong paper size setting is used, results
on the report printout might be missing.
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2.3.2 Startup the instrument & Print the Self Test

CAUTION! The instrument check /set up must be done in Engineering mode
(see page 3-37). This mode must be de-activated at the end of the
service.
• Power on the instrument.
• Log as "super" administrator for Windows and DAVINCI QUATTRO
Software.
• Start the DAVINCI QUATTRO Software in Engineering mode.
The PC and the instrument will “connect” and a self-test procedure takes 60-80
seconds. The arm will move during this time, so the cover must be closed, or the
safety key inserted and safety precautions observed.
When the tests are complete, the test results will appear on the monitor. Examine
the results to make sure all modules passed the self-tests. Use the print command
to print a copy of the initial self-test results (3 pages). The data can also be saved to
a file in the folder :
C: \ Program Files \ bioMérieux \ DAVINCI QUATTRO Program \ Selftests \
filename.tst
2.3.3 Language selection (optional)

Refer to the DAVINCI Quattro Computer Installation procedure booklet.
2.3.4 Print the workspace calibration data and the module

coordinates
From the “Tools” pull-down menu, select “Configure System”. Then, select
“Coordinate Calibration”. A screen will appear and show all the XYZ module / drawer
coordinates.
Use the “Print” button to make a printout of the workspace calibration data and the
module coordinates. Preserve this initial copy of the settings in case recalibration of
modules is necessary.
Note: The “Print” button is only present in Engineering mode.
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2.3.5 User administration (optional)

To create a user account:
• From the main menu, select “Tools” – “Edit User Access”.
• To add new users, click “Add”.

• Enter the user name and click “OK”.
• The edit User dialog box is displayed.
− Enter as user description e.g. operator. This is optional.

− Enter as password and confirm the password.
− Set the access mode “Operator” (user) or “Administrator” (lab
manager).
− Click Save.
NOTE: The password is case sensitive (The user name is not case
sensitive).
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2.3.6 Setup Laboratory Information to appear at the top of

the result reports
• Select Tools - Options from the main menu and then select Laboratory
Information tab to enter laboratory demographics to appear at the top of
the results printouts.
• Enter the laboratory name, address, telephone number and fax number
and then click “OK”.
2.3.7 Setup the Sample Identification Module

A form with the specific X, Y, and Z coordinates for optimal scanning is included with
every SID (every instrument), see next page. These values should be entered into
the QUATTRO Software during initial installation. These values will replace the
default values used in the software. For the plate scanner the default settings can be
used (8816, 1385, 2700 - 538).
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1 SMF1698
8440 1260 750
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2.3.8 Install the assay protocols (depending on the

customer needs)
The files have to be copied to the folder:
C :\ Program Files \ bioMérieux \ DAVINCI QUATTRO Program \ Assays
2.3.9 Setup the correct sample tubes and caddies

Ensure that the tubes used for the samples are exactly the ones the system is
configured for (3 default settings are present in the database). If the value has to be
adjusted, create a new sample tube and a new caddy. Especially, the internal
diameter needs special attention. The sample tube might be tapered. The lowest
measured internal diameter must be used.
Use sample racks with spacer (art code 200415) for sample tubes
with a height of 80 mm or less.
Use sample racks without spacer (art code 200414) for sample
tubes with a height of more than 80 mm.
NOTE: It is not possible to permanently modify the bioMérieux predefined
sample tubes and sample tube caddies. After restarting the database,
the original predefined settings will be given.
1. Create new sample tube (optional)
Tools -Edit Databases -Tubes Database
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2. Create new sample tube caddy

Tools -Edit Databases -Sample Racks Database
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Select the new sample tube
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Mechanical tests
2.4 MECHANICAL TESTS
2.4.1 Set the deepwell dilution plate or deepwell dilution

strips
Before using the Cycle-Test-2 protocol, the DAVINCI QUATTRO must be set to
deepwell dilution plate or deepwell dilution strips. Otherwise, crash will happen !!!
− Click on the drop-down menu located below the WashDisp button.

− Select "Settings" and then "Runtime Options…".
− Select the proper "Dilution Plate", 1ml dilution plate (deep well plate) or
dilution strip plate (deep well dilution strips) according the customer needs.
− Click OK.
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Mechanical tests
2.4.2 Run the Tip Alignment Test

This test will assure that the arm is correctly picking up tips from all tip racks. The
installer should watch as the test runs to assure that the pipette is centering
correctly and smoothly picking up pipette tips.
− Install the 2 deep well plates.
− Install reagent tips (41) and sample tips (4 x 108).
− Ensure no sample tubes are loaded because the tip will not center on
the tubes. (If a sample tube is present, the tip might hit the tube.)
− Remove any control bottle from the control rack because the tip will not
center on the bottles.
− To start the test, select “System Manual Control” from the “Tools” pull-
down menu.
− Select “Yes” to continue when a warning appears concerning the use of
manual control.
− Click on “Testing” and choose “Tip alignment tests”. Select “Do It”.
− The following screen appears. The test depth can be selected between
0 and 30.
Use the default setting of 10. Tip types, Tip racks and Target racks
need not be changed.
The settings have no influence on the test. Select “Go”.
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Mechanical tests
− The following screen appears. Load 41 Reagent Tips in Reagent Tip

Rack 1 (only 4 tips are used).
Press the button to continue.
− Load 108 sample Tips in Sample Tip Rack 1, 2, 3 and 4 (only 4 tips are
used).
Press the button to continue.
Ensure the tips are correctly picked up. Ensure the tip is centered in the deep well
plates or strips. The tip will not be centered to sample tubes and control vials.
− Recalibrate the workspace, if necessary.
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Mechanical tests
2.4.3 Run the Sample Rack Pickup & Vertical Barcode

Scanner Test
Perform the following tests to assure that the arm is correctly picking up sample
racks and the SID is functioning.
down menu.
manual control.
− Click on “Testing” and choose “Sample rack scanning tests”. Select “Do
It”.
− Select any or all of the Sample racks (cycles to complete = 1). Select
“Go”.
The machine will sequentially pick up all racks and move them in front of the vertical
barcode reader. The readings are not displayed and no printout will be generated.
Visually watch and listen for smooth operation of the pick-up of the racks.
The scanned Sample ID’s can also be written to a file by activating the checkbox:
C: \ Program Files \ bioMerieux \ DAVINCI QUATTRO Program \ Sample ID Cycles \
Cycle0000001.txt
NOTE: The option “Write Engineering Test Results Document” is only active
when logged on in engineering mode.
If you observe rack pickup problems, recalibrate the workspace

(see 7.1.3).
If you observe barcode reading problems,
− verify if the type of barcode has been enabled (see 7.3.1).
− adjust the scan position (see 7.3.4).
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Mechanical tests
2.4.4 Run the Plate Movement Test

This test is performed to assure that the instrument arm is correctly picking up all
plate carriers and placing them precisely into the various modules and drawer
locations.
While performing the test, observe and listen as the arm picks up and places
carriers for any indications that the positions are not correctly set.
down menu.
manual control.
− Click on “Testing” and choose “Plate movement”. Select “Do It”.
− Enter the number of times to perform the Cycle Count and select “Go”.
At least 12 cycles should be chosen for a complete run. All modules will
be tested 2 times.
e.g. 12 cycles
Cycle 1 : pos 1 => inc 1 => pos 2
Cycle 5 : pos 1 => reader => pos 2
Cycle 6 : pos 2 => washer => pos 3
Cycle 11 : pos 3 => reader => pos 4
Cycle 12 : pos 4 => washer => pos 1
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Mechanical tests
Each cycle involves the movement of the plate from the ambient drawer to another
module and back. Each movement should be smooth (i.e. no jerking, hesitation or
interference). Calibrate the modules if the movement is not smooth.
− Place a plate carrier in the front left drawer position (position 1).
− Close the cover. The testing will start.

− Repeat this test for the 3 other plate carriers. That means 48 cycles (4 x12
cycles) will be performed.
NOTE: If the cover is not closed, an alarm will be generated and the following
error message will be displayed :
To mute this alarm and to continue the testing, select “Recovery Options” -
“Try to move the z-motor to 3240 again”. Select “Recovery Options” and
“Try to move the z-motor to 3240 again” again. After selecting this option twice, the
testing will continue.
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Mechanical tests
2.4.5 Run the Horizontal Barcode Scanner Test

− To start the test, select “System Manual Control” from the “Tools” pull-down
menu.
manual control.
− Select “Drawer” and “Eject”. Select “Do It”.
− Load a plate carrier with a barcoded microplate (long side of microplate) in
position 1.
− Click on “Testing” and choose “Plate scanning tests”. Select “Do It”.
− Select Cycle Plate 1. Select “Go”.
The machine will pick up the plate and move them in front of the horizontal barcode
reader. The readings are not displayed and no printout will be generated.
The scanned Plate ID can be written to a file by activating the checkbox :

C: \ Program Files \ bioMérieux \ DAVINCI QUATTRO Program \ Plate ID Cycles \
Cycle0000001.txt
If you observe barcode reading problems,

− verify if the type of barcode has been enabled (see 7.3.1).
− adjust the scan position (see 7.3.4).
®
Instrument qualification
2.5 INSTRUMENT QUALIFICATION
2.5.1 Dprog manual entry

• Enter all the required data about the diagnostic kit and the OPSYS plate
(blue cells).
Note: The required calibration data is on the OPSYS calibration certificate.
Dates must be entered in following date formats : DD/MM/YYYY or MM/YYYY.
Diagnostic kit
OPSYS plate
Check that these values are the

values set in the DAVINCI
QUATTRO. This operation must be
done when performing the Default
parameters setting (see page 2-35).
2.5.2 Reader Verification

There are no calibrations to perform on the reader.
The filter setting must match with the filters installed into the reader.
After changing the lamp, it might be possible that the selftest fails.
To fix this problem the lamp position has to be changed. To find the
correct lamp position check the reader outside the instrument by
means of the Extension Cable Tool (art code 45688562). Change the
position until the selftest is OK.
To verify the reader module, 2 tests can be performed :

OPSYS (Reader Verification Plate test)
C2C (Channel to Channel variation test)
IMPORTANT! Shut down (!! Not restart) the QUATTRO and computer and restart
both systems (if not, OD's of the verification plate can not be
stored).
®
2.5.2.1 OPSYS (Reader verification plate test)

Prerequisite:
− OPSYS tool (art code 45688568)
This package contains also a diskette and an operating manual. These 2 parts can
be discard because we do not use them anymore. Use the following procedure to
verify the reader.
Ensure the OPSYS plate is installed flat and in the correct orientation in the plate
carrier. Ensure that rows in row A and H are unobstructed and the plate glass is
clean. Make sure the filters are free of dust, oils, scratches and contamination.
Never touch the surface of the optical filter media. Keep the plate away from direct
sunlight.
• Select: File -> New -> Worklist

• Select: Add Assays using a new batch of samples then press OK
• Select: New plate -> Select the D4_UK_OPSYS_2_QP_02.asy in the list
and press the Open button.
• Click with the right mouse button on the first position of the sample ID list
• Select 96 samples.
• Press the Auto Assign Sample ID button and then press OK.
• Start the run by pressing the green triangle button.
• Press the ‘Fast Forward’ button.
• When the Lot Specific Data and Runtime Variables screen appears, press
the green ‘V’ button.
• When the Load Sample Rack screen appears, press the green ‘V’ button.
• Load the OPSYS calibration plate on the correct drawer position enter a
plate identifier in the entry box and then press the green ‘V’ button
• Check waste containers if necessary empty them and press OK.
• After the run is finished the results file can be found in the following folder
C:\Progam Files\bioMérieux\DAVINCI QUATTRO Program\WFV
®
• The file that was generated must be imported in Excel as follows:

• Start the Excel program
• Select File Open (*.* all files)
• Use the Browse function to select the file to be imported
• Select Open and use the following settings in the Text Import Wizard.
• Select Delimited -> Next
• Select Tab, Comma, and Space then press the Finish button.
• Copy Cells A1 until M360
• Paste these cells in the ‘OPSYS’ sheet of the Dprog excel file starting
at cell A1.
• Check the 'Results' sheet of the Dprog excel file: no red cells.
®
Example of the OPSYS verification file:
®
®
®
Cleaning of the OPSYS Plate

A DRY source of compressed air may be used to remove the dust from the filters. If
it’s not sufficient, use a clean swab or optical cloth moistened with spectrograde
methanol. Let the plate dry for 45 minutes in open airspace before continuing (no
sunlight !).
What kind of checks are performed ?
C1 C2 C3 C4 C5 C6 C7 C8 C9 C10
S1 S1 S1 S4 S4 S4
S2 S2 S2 S5 S5 S5
S3 S3 S3 S6 S6 S6
S7 S7 S7 S10 S10 S10
S8 S8 S8 S11 S11 S11
S9 S9 S9 S12 S12 S12
T1 T1 T2 T2 T3 T3 T4 T4 T5 T5 T6 T6
When the reader verification test fails, the complete reader module has to be
replaced.
Plate recalibration and certification

Annual re-certification of your verification plate is recommended. The calibration due
date is on the plate and on the certificate of calibration. The plate has to be sent to
Logistics France for recalibration. It will take about 6 weeks before the plate will be
returned. Request first a Return Authorization Reference (document CF00142).
The re-certification process uses dedicated, traceable standards and
instrumentation at Dynex. These standards are periodically and rigorously
characterized by both the National Institute of Standards (NIST) in the United States
and the National Physics Laboratory in the U.K. (NPL) to ensure the quality of our
services and your results.
®
2.5.2.1.1 C2C (Channel to channel variation test)
To check the channel to channel variation of the reader, run the C2C protocol.
Prerequisite:
1 reagent bottle with 23 ml wash buffer
1 Break-4 empty plate
CAUTION! When loading liquids, make sure that no air bubbles are present in
the bottles and tubes.

• Select: New plate -> Select the D4_UK_C2C_2_QP_02.asy in the list and
press the Open button.
• Load a clean and Break-4 empty plate on the correct drawer position enter
a plate identifier in the entry box and then press the green ‘V’ button
• Load the requested reagent containers with sufficient reagent and tips.
C:\Progam Files\bioMérieux\DAVINCI QUATTRO Program\Text
• Paste these cells in the ‘C2C’ sheet of the Dprog Excel file starting at
cell A1.
®
Example of the C2C verification file:
®
2.5.3 Calibration plate

Prerequisite:
Reagent bottle with 15 ml wash buffer
Low density solution (12 ml)
To be able to calculate the results for the sample and reagent verification, a
reference plate is needed.
• Prepare the reference plate. Use a calibrated pipette to fill a Break-4 plate
with 80 µl low density in row 4-5-6, 160 µl in row 7-8-9 and 240 µl in row
10-11-12. Leave row 1-2-3 empty.
1 2 3 4 5 6 7 8 9 10 11 12
A 80 80 80 160 160 160 240 240 240
B 80 80 80 160 160 160 240 240 240
C 80 80 80 160 160 160 240 240 240
D 80 80 80 160 160 160 240 240 240
E 80 80 80 160 160 160 240 240 240
F 80 80 80 160 160 160 240 240 240
G 80 80 80 160 160 160 240 240 240
H 80 80 80 160 160 160 240 240 240

• Select: New plate -> Select the D4_UK_DRP_2_QP_02.asy in the list and
• Load carefully the reference plate prepared at first step on the correct
drawer position, enter a plate identifier in the entry box and then press the
green ‘V’ button.
®

• Paste these cells in the ‘DRP’ sheet of the Dprog Excel file starting at
cell A1.
If so, redo the calibration plate.
Example of the DRP verification file :
®
2.5.4 Pipette volume calibration

NOTE: The pipette calibration is not mandatory in case of preventive
maintenance (mandatory for installation).
2.5.4.1 Principle
The manufacturer is using special tools (Artel reader, software and liquids) to
calibrate the delivered volumes of the pipette. The procedure takes several hours
and can not be done in the field. However, we encountered that the volume
dispensed by the calibrated pipette is 10 % lower than expected. The manufacturer
is using a calibration method based on water.
For serum, plasma or reagents the volume is a little bit lower (approximately 10 %).
Most of the pipette modules are using the same default calibration factors. These
values can be entered manually. To compensate for the lower volume, the
calibration factors have to be changed. This must be done during the installation of
the instrument. After entering the correct values, a pipette verification procedure
must be performed. When the pipettor verification fails, the calibration factors need
to be adapted and the pipettor verification has to repeated.
Example : Calibration curve Sample Dispensing Single Shot
Calibration Curve
200.000
Actual Volume
150.000
100.000
50.000
0
5 10 20 30 50 100 150 200
Target Volum e
®
2.5.4.2 Default parameters setting

1. From the main menu select Tools – Calibrate Pipette. The following screen
appears.
2. Enter the coordinates X=4000, Y=1000 and Z=2500 and press the button
“Show XYZ”.
The pipettor will move to this position.
3. Click on "Next".
4. Enter the "Actual Volumes" in the following screens. To do,
− Select the target volume.
− Click on "Edit" and press "OK".
− Then, the "Actual Volume" must be set to ** NOT CALIBRATED **
NOTE: To add or remove volumes, click on the "Add" or "Remove" button.
The choice of the Calibration Liquid is not important.
− When all "Actual Volumes" have been set to "NOT CALIBRATED", click on
"Next".
Sample tip volumes for single shot
®
Reagent tip volumes for single shot
Sample tip volumes for multiple shots

The multiple shot function for samples is not used. That means we do not have to
change these settings. Press “Next” to continue.
®
Reagent tip volumes for multiple shots
5. When having entered the previous screens, the following screen appears.
A. Change the "Number of cycles to average each volume" from the default
5 to just 1.
B. Tick the box to "Enter values manually".
C. Untick the box to "Remind the user to open / close the Artel lid".
This button is only

A available in
engineering mode.
You will be asked
to enter your name
and serial number
of the system.
C
B
6. Press “Finish” to continue. Now, you will be requested to enter the following
calibration factors for all sample and reagent volumes in single shot and
multiple shot. This should be done one by one.
®
7. Enter the volume and press "OK".
®
Calibration Volumes:
• Sample tip volumes for single shot
5 µl 3.007
10 µl 7.351
20 µl 15.554
30 µl 24.641
50 µl 41.910
100 µl 86.024
150 µl 129.573
200 µl 174.715
• Reagent tip for single shot
25 µl 18.574
35 µl 26.907
55 µl 44.035
100 µl 83.466
150 µl 126.730
200 µl 172.106
250 µl 217.296
300 µl 261.188
• Sample tip for multiple shot (not used on QUATTRO)
We do not use the multiple shot function for sample tips. Therefore, we are not
going to change the default values.
For parallel sampling we are using single shot. The disadvantage is that it takes
some more time. The pipettor is moving with the same tip, two times to the sample
tube.
75 µl 25.850 (normalized factor = 0.967)

300 µl 42.749 (normalized factor = 1.170) (= factor of 50 µl)
®
• Reagent tip for multiple shot
Example : 125 µl 27.241 (normalized factor = 0.918)
125 µl is the volume still present in the tip when dispensing 25 µl. The dispensed
volume is measured and entered in the screen e.g. 27.241 µl. The normalized factor
is calculated by the software and displayed on the screen e.g. 25 divided by 27.241
= 0.918.
volume in the tip measured volume @ 25 µl

8. After entering all values, the system will store the values into the EEprom in
the pipette module. Wait. After a few seconds, the main screen appears
again.
®
2.5.4.3 Sample calibration
CAUTION! Before launching the calibration, make sure that the calibration
factors have been set to default values in the DAVINCI QUATTRO (see
Default parameters setting on page 2-35).
Prerequisite:
1 sample tube with medium density solution
1 control bottle 2 ml low density
1 control bottle 2 ml medium density
1 reagent bottle with 25 ml wash buffer
To check the Pipette sample volume calibration,run the Calib protocol:

• Select: New plate -> Select the D4_UK_Calib_2_QP_02.asy in the list and
• Select 1 sample.
• When the Load Sample Rack screen appears load the sample (medium
density) and press the green ‘V’ button.
• Load a Break-4 empty plate on the correct drawer position enter a plate
identifier in the entry box and then press the green ‘V’ button
• Paste these cells in the ‘Calib’ sheet of the Dprog Excel file starting at
cell.
• Check the accuracy and precision for Calib in the sheet ‘Results’ in the
Dprog excel file.
®
If one accuracy or precision is not met (1 yellow cell at least), enter all new
calibration values according to the values indicated in the sheet (see chapter 15 -
Addendum: Pipetting qualification).
IMPORTANT! Never repeat the calibration.
Values entered in Dprog manual entry and calibration factors

entered in the DAVINCI QUATTRO must be the same before
performing the calibration.
Example of the calib verification file:
2.5.4.4 Reagent calibration (optional)

See chapter 15 - Addendum: Pipetting qualification.
®
In engineering mode you have the possibility to print the results (see next pages).
From the print screen the values can also be saved to a text file.
bioMérieux Test Results
Date: Friday, April 14, 2006
User Name: Smith

Product: DAVINCI QUATTRO
Product Serial Number: 1113
Pipette calibration data
Serial number: 1PMB2277

Last date pipette calibration updated: Friday, April 14, 2006
Back lash compensation steps: 5
Dispense blow out steps: 100
Aspirate air gap steps < 150 µl: 30
Aspirate air gap steps >= 150 µl: 150
**** Single shot dispense calibration data ****
Single shot dispense sample tip data

Requested volume (µl) Actual volume (µl)
5 3.007
10 7.351
20 15.554
30 24.641
50 41.910
100 86.024
150 129.573
200 174.715
Single shot dispense reagent tip data

Requested volume (µl) Actual volume (µl)
25 18.574
35 26.907
55 44.035
100 83.466
150 126.730
200 172.106
250 217.296
300 261.188
®
**** Multiple shot dispense calibration data ****
Sample tip aspirate volume: 300 µl

Reagent tip aspirate volume: 1200 µl
Dispense volume: 25 µl
First shot dispense volume: 50 µl
Sample tip buffer volume: 50 µl
Reagent tip buffer volume: 100 µl
Muliple shot dispense sample tip data

Requested tip volume(µl) Adjusted tip volume(µl) Normalised factor
75 70.721 0.967
200 192.101 0.980
225 216.874 0.995
250 241.388 0.985
300 300.000 1.170
Muliple shot dispense reagent tip data

Requested tip volume(µl) Adjusted tip volume(µl) Normalised factor
125 203.697 0.918
250 318.433 0.924
350 410.128 0.919
450 501.161 0.911
550 592.770 0.925
700 730.283 0.917
900 914.337 0.929
1050 1054.754 0.948
NOTE: From software version 6.10a onwards, a new feature is availble under
Tools in Engineering mode, "Calibrate irregular Bottles". This
function is not used by bioMérieux.
®
2.5.5 High volume Air Gap check

• Select “System Manual Control” in “Tools” area
• A message informs you that Manual Control is advanced feature, click
“Yes”
• Select “Testing” and “Dispense Test” and click on “Do it”.
The following screen appears.
• Check that the values are:

Low = 30
High = 63
• Click on "Finish".
2.5.6 Pipettor Verification

To check if the pipettor is dispensing the correct sample and reagent volumes, a
pipettor verification is performed.
2.5.6.1 Sample Verification (single shot)

Prerequisite:
1 sample with medium density
2 controls with low density (4 ml + 2 ml)
1 control with medium density (2 ml)
1 reagent buffer (23 ml)
To check the sample, run the SampVer protocol:

• Select: New plate -> Select the D4_UK_SampVer_2_QP_01.asy in the list
• Select 1 sample.
®

• When the Load Sample Rack screen appears load the sample (medium
• Paste these cells in the ‘SampVer’ sheet of the Dprog Excel filestarting
at cell A1.
®
Example of the sample verification file:
®
2.5.6.2 Reagent Verification (multilple shot)
Prerequisite:
1 reagent with low density (10 ml)
1 reagent with medium density (2 ml)
1 reagent wash buffer reagent (17 ml)
To check the reagent, run the ReagVer protocol:

• Select: New plate -> Select the D4_UK_ReagVer_2_QP_03.asy in the list
• Paste these cells in the ‘ReagVer’ sheet of the Dprog Excel filestarting
at cell A1.
• Check the 'Results' sheet of the Dprog excel file: no red cells. If the results
are not met, see chapter 15 - Addendum: Pipetting qualification.
®
Example of reagent verification file :
®
2.5.7 Wash head offsets

This calibration procedure will level the manifold in the horizontal plane and center
the wash head needles in the center of the well by define the X offset for the arm
and the Y offset for the tray. The procedure will also define the bottom position of
the plate with a correction for the left (strip 1) and right side (strip 12) of the plate
(respectively Z1 and Z12 offsets).
The offset range is from 0 to 100. Negative offset values are not allowed. This can
be critical for the z values. Change the Z home sensor position, if obtaining negative
values.
Material required: Washer Alignment Fixture (art. no 45688569)
1. Restart the computer and the QUATTRO system. Start the Washer
Calibration software (Start\Programs\Dynex Technologies\dsx Washer
Calibration)
®
All fields must be entered before you can continue. Most information is only useful
for the printout (only density is important when calibrating the bottles).
− Enter your name as Operator Name.

− Enter the room temperature in °C. This will be used to calculate the
water density. The program automatically calculates water density
based on the specified room temperature. However, this option is NOT
implemented in the current software version.
− When a cylinder is used to measure the volume enter a “x” for the
Balance serial number and the Balance Calibration Expiration Date.
− The serial numbers of the bottles can be found on the Instrument
Configuration Report delivered with the instrument. (when not available,
enter “x”).
− To change the Fluid and Fluid density, click the Custom button.
− When using a balance and water (DI water), use the default density of
0.9982 g/ml.
− When using a balance and wash buffer, enter 1.006 g/ml for density.
− When using a cylinder to measure the dispensed volumes, always
enter 1 g/ml for density.
− The default setting for the communication port is Com 1. Do not
change.
− The default setting for “Aspiration start X position” is “Well Center”. Do
not change.
− The file save path must not be changed.
− Ensure the instrument is on before pressing the OK button.
2. After pressing OK, the following screen appears. Perform the displayed steps
and press OK.
®
3. The washer calibration and verification screen appears.
NOTE: Remark : When the program is started and before any verification
activities have occurred, the words "Not Tested" or “Not calibrated”
will appear. After verification or calibration this text will change to
"Pass" or "Fail" or “Calibrated”, depending on the outcome of the
action.
All test results will be logged and may be viewed, printed or saved.
When starting the washer software again, the status will again be
reset to "Not Tested" or “Not calibrated”. This is normal. It does not
mean that the pump has not been calibrated.
4. Select “Calibrate Head Offsets“ from the washer calibration menu. The
following screen appears. Insert the washer alignment fixture with the two
alignment grooves to the top and on the left and press OK.
®
5. The wash head moves to the first strip position. Ensure the head is level to
the jig (screw on top of the manifold). Press OK when done.
Screw
6. The following screen appears. Adjust the Z position to define the Z1 offset
position
Press OK when the needles are touching the top of the test jig.
When pressing DOWN, the number of

Z steps will increase.
Z1 offset = Z1 top of jig - 35
Screen shows the actual Z1 position of

the arm.
This value must be at least 35.
®
7. The wash head moves to strip position 12. Ensure the head is level to the jig
(screw on top of the manifold). Press OK when done.
8. The following screen appears. Adjust the Z position of the wash head to
define the Z12 offset position. Press OK when the needles are touching the
top of the test jig.
When pressing DOWN, the number of

Z steps will increase.
Z1 offset = Z1 top of jig - 35
Screen shows the actual Z12 position

of the arm.
®
9. The wash head moves to strip position 6 to define the Y offset of the tray.
The following screen appears. Press OK when the aspirate pin is centered
over the groove.
When pressing IN, the number of Y steps

will decrease.
Y offset = 30 - Y center position
Screen shows the actual Y position of the tray.
10. The wash head will stay to strip position 6 to define the X offset of the tray.
The following screen appears. Press OK when the needles are centered over
the left groove.
When pressing LEFT, the number of Y

steps will decrease.
X offset = X center position - 820
Screen shows the actual X position of the arm.
®
11. The new offsets will be displayed. Press OK to continue
NOTE: a Z value lower than 35 steps results

in a negative value. The software
does not accept a negative value for
the offset.
When having a negative offset, the
distance between the z home position
and the top of the tool plate has to be
increased. This can be done by
changing the position of the z-sensor
(2 screws) or by lowering the block
that activates the z-sensor (2
screws).
An easy and successful solution is to
remove some material from the block
by means of a knife. A small part
might be enough.
12. Press “Yes” to save the new offsets to the EEPROM on the washer main
board.
13. Remove the calibration tool and press OK to continue.
14. You will be informed that the home offset calibration is complete. Press OK.
®
Verify Head Offsets
After calibrating the offsets, we recommend to run the “Verify Head Offsets”.
1. Select “Verify Head Offsets“ from the washer calibration menu. The following
screen appears. Insert the washer alignment fixture upside down and press
OK.
2. The wash head needles must move above the calibration plate without
touching.
When finished the following screen appears. Press Yes or No.
3. Remove the calibration plate.
4. You will be inform that the verification is complete. Press OK.
You can always view results, print or save the reports.
CAUTION! After the new offset values are saved to the EEPROM, the
instrument must be powered off and back on to use those new
values.
®
2.5.8 Washer Pump calibration A, B, C, D
The washer will dispense fluid into a low sided vessel (art. code 45688570) and the
operator weighs, or measures the volume of the fluid.
The program converts weight to volume. Two calibration points are used to generate
a linear equation that describes the calibration of a bottle. The calibration values for
a bottle include the pump speed, the slope and intercept of the line describing the
calibration.
When a balance is not available, you can also use a cylinder to measure the
dispensed volume.
The washer module will move the dispense head over to the middle of the fluid
reservoir, dispense, move slightly left and then return to dispense more fluid. All in
all, five fluid dispenses occur for each of the two calibration points in order to
minimize dispense-to-dispense errors.
The dispense speed is fixed at 190. The time that the pinch valve is open is
calibrated.
Each bottle has to be calibrated although only 1 pump is used. Due to a difference in
tubing length between the bottle and the pump, we observed that the dispensed
volume for bottle D is little bit higher (shorter distance). The influence of a different
pinch valve for each location is negligible.
Volume
(µl)
474 1794 Time (ms)
®
Prerequisite:
− Calibrated gravimetric balance with 0.01g resolution (minimum range:
0.01 - 100.00g) or a 10 ml cylinder and a 50 ml cylinder to measure the
dispensed volumes.
− Deionized water or wash buffer.
− A low-sided vessel capable of containing approximately 50ml of fluid
without spillage (5 vessels are included in the sales package).
Procedure to calibrate a pump (bottle)

1. Start the Washer Calibration software. The following screen appears.
All fields must be entered before you can continue. Most information is only useful
for the printout (only fluid density is important).
− Enter your name as Operator Name.
− Enter the room temperature in °C. This will be used to calculate the
water density. The program automatically calculates water density
based on the specified room temperature. This option is NOT
implemented in the current software version.
− When a cylinder is used to measure the volume enter a “x” for the
Balance serial number and the Balance Calibration Expiration Date.
− The serial numbers of the bottles can be found on the Instrument
Configuration Report delivered with the instrument. (when not available,
enter “x”).
− To change the Fluid and Fluid density, click the Custom button.
®
• When using a balance and water (DI water), use the default density of
0.9982 g/ml.
• When using a balance and wash buffer, enter 1.006 g/ml for density.
• When using a cylinder to measure the dispensed volumes, enter 1 g/ml for
density.
− The default setting for the communication port is COM 1. Do not

change.
− The default setting for “Aspiration start X position” is “Well Center”. Do
not change.
− The file save path must not be changed.
− Ensure the instrument is on before pressing the OK button.
2. After pressing OK, the following screen appears. Perform the displayed steps
and press OK.
®
The washer calibration and verification screen appears.

3. Select the button “Calibrate Bottles” from the washer calibration window.
4. Enter the letter corresponding to the pump to calibrate and press OK.
5. Select “Yes” to prime the washer. Prime the washer until no air bubbles are
present in the fluid line between the dispense bottle and the wash head.
Starting with empty tubing, it’s necessary to prime the washer at least 10
times to get rid of the air. Press the “No” button when the priming is fine.
6. Perform the instruction on the screen. When a cylinder is used to measure

the volume, just put the low-sided vessel in a plate carrier in the washer tray.
Press OK.
®
7. When “Perform the first dispense ?” is presented, answer “Yes”.
8. Press OK to start dispensing. The fluid will be dispensed in 5 steps. A low

volume will be dispensed.
9. When finished, remove the low-sided vessel and press OK.
10. Enter the net weight in grams and press OK. When using a cylinder, enter
the volume in ml e.g. 8.8
Note: To enter a volume with a decimal, always use the full stop to create the decimal
(never use a comma!).
®
11. Follow the instructions provided by the program and press OK.
12. To start the second dispense step, press Yes.
13. Press OK to start dispensing. The fluid will be dispensed in 5 steps. A high
volume will be dispensed.
14. When finished, remove the low-sided vessel and press OK.
15. Enter the dispensed volume and press OK. When using a cylinder, enter the
volume in ml e.g. 35.9
Note: To enter a volume with a decimal, always use the full stop to create the decimal
(never use a comma!).
®
16. The program will calculate and display the slope and intercept values.
Select OK to continue.
• The slope must be between 0 and 6.5 (typically 1.5 to 4.5 but if your
values are out of this range, it may indicate a pump weakness).
• The intercept must be between 0 and 512 (typically 20 to 70 but if your
values are out of this range, it may indicate a pump weakness).
17. Save the values to EEPROM when the new values are in range. Select Yes.
18. Repeat the pump calibration for bottle B, C and D.

Due to a difference in tubing length between pump and bottle, we observed
that the delivered volume for bottle D (shorter tubing) is a little bit higher than
for bottle A. Therefore, it’s recommended to calibrate all 4 bottles in stead of
copying the values to the other bottles.
NOTE: After new slope / intercept values for any pump are saved to the
EEPROM, the instrument must be powered off and back on to use
those values before any additional testing is performed on that pump.
− By pressing the “View results” button, the information can be displayed.
− By pressing the “Print” button, the information can be printed.
− By pressing the “Save” button the information can be saved to a file
1WMA1867_CalBottles.txt. in the folder C:\ Program Files \ DSX Washer Calibration
®
Example of printout:
Operator Name : Marc

Room Temperature (°C) : 20
Balance Serial Number : x
Balance Calibration Expiration Date : x
Bottle A Serial Number : 2836214
Bottle B Serial Number : 2836215
Bottle C Serial Number : 2836216
Bottle D Serial Number : 2836217
Signature __________________________________________
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16/12/2005 11:47:57
DSX Washer Module Pump Calibration Procedure : 1 x 8 Manifold, Pump : A
DSX SN : 1EPA1871, Washer Module SN : 1WMA1867, Washer Firmware: WM1.15
Formulas :
y=mx+b (m=slope, b=intercept, y=time, x=volume)
m=(y2-y1)/(x2-x1) and b=y1-m*x1
Washbuffer density @ 20°C = 1.0000g/ml
g to µl conversion : [ weight(g) * 1000µl/ml ] / [#DispensePins * density(g/ml) ]
Original Slope = 2.2 Original Intercept = 34
First dispense ran pump at speed 190
Second dispense ran pump at speed 190
Target Volume1 = 200µl, Time1 = 0474msec
Target Volume2 = 800µl, Time2 = 1794msec
In Process Results :
Reported Weight1 (for 5 replicate dispenses) = 8.75g
Reported Weight2 (for 5 replicate dispenses) = 35.90g
Actual Volume1 = 218.8µl/well
Actual Volume2 = 897.5µl/well
Calculated Calibration Values:

Pump Speed: 190 - Normal Speed (This value is constant.)
Calculated Slope : 1.9 (Acceptable range: 0 < slope <= 6.5)
Calculated Intercept :58 (Acceptable range: 0 < intercept <= 512)
CALIBRATION VALUES to be saved in memory :
Slope = 38 (= calculated decimal slope x 20 because the saved value must be an integer)
Intercept = 58
Calibration Result : COMPLETE (new calibration values saved)
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2.5.9 Washer Verification

2.5.9.1 Residual volume (WRV)
Prerequisite:
Reagent bottle with high density solution (12 ml)
Distilled water in the required wash container
Washbuffer (240µL/well) in 2 reagent bottles (24 ml + 4 ml).
This test must be performed with WRV protocol:

• Select: New plate -> Select the D4_UK_WRV_2_QP_03.asy in the list and
• Load the requested washbuffer/reagent containers with sufficient reagent
and tips.
• Paste these cells in the ‘WRV’ sheet of the Dprog Excel filestarting at
cell A1.
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Example of the WRV verification file:
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2.5.9.2 Fill Verification (WFV_X)

This test must be performed for all 4 bottles with WFV_A, WFV_B, WFV_C and
WFV_D protocols.
Each well will be filled with 240 µl.
Prerequisite:
Low Density Color solution (400 ml for each line)
4 protocols in the Assay directory
There are 4 protocols (X= A, B, C or D) to perform.

• Select: New plate -> Select the D4_UK_WFV_X_2_QP_02.asy in the list
• Paste these cells in the ‘WFVX’ sheet of the Dprog Excel filestarting at
cell A1.
This procedure must be applied for each line A, B, C and D.
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Example of the WFVX verification file:
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2.5.10 Incubator verification

Prerequisite:
IVT ref 284119 : Incubation Verification Tool with its RS232 serial
cable
Logger software installed on an external PC if using DC7100.
A. IVT initialization
• Switch on the IVT.
• Connect IVT to the RS232 serial cable provided : mind connection
correspondence between the 2 red points on connectors.
• Connect the RS232 serial cable to the COM4 port of the PC.
• Start LOGSOFT to launch the Logger software.
• Make sure that COM4 is selected in File/Preferences.
Note: Depending on some configurations, this message may appear. Click “OK”.
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The following window is displayed :

• connect Serial cable to another port and choose the other corresponding
port below, and click “Retry”.
• If connexion is available between PC and IVT, the following Logger

Software screen will appear : Select “Logger” and click on “Launch.
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• Make sure the Description field is complete in the following window:
• Click on “Start”, and wait for following message :
• Unplug the RS232 serial cable from the IVT.

• Click “OK”.
• Exit from Logger Software.
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B. Load IVT in the incubator

• Insert IVT in a plate support.
• Open Da VINCI QUATTRO Program with operator logon (instrument must
be ON)
• Select “System Manual Control” in “Tools” area
• A message informs you that Manual Control is advanced feature, click
“Yes”
• Place IVT inside each incubator to register a temperature incubation cycle
for each :
for example for incubator 1:
1. Select “Incubator 1” and “Eject” and click on “Do it”.
2. Place the Plate support with IVT on incubator tray.

3. Keep “Incubator 1” selected and select “Move to heater”, then click on “Do it”.
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4. Select “Advanced” and “Send a command(s)”, then click on “Do it”.
5. Type I0HTR1370 and click “Send” to set incubator 1 bottom heater (1 : plate
heater) to 37.0 deg.
6. Type I0HTR2370 and click “Send” to set incubator 1 top heater (2 : cover
heater) to 37.0 deg.
7. Count 30 minutes for the IVT to reach at temperature and stabilize.

8. Type I0HTR1250 and click “Send” to switch off the incubator 1 bottom
heater.
9. Type I0HTR2250 and click “Send” to switch off the incubator 1 top heater.
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10. Click on “Close”.

11. Select “Incubator 1” and “Eject” and click on “Do it”.
12. Remove IVT from incubator 1 tray.
13. Keep “Incubator 1” selected and select “Move to heater”, then click on “Do it”.
14. Let IVT at ambient temperature for 15 minutes.
• Repeat operations from 1. to 14. Incubator 3 Incubator 4

for each incubator:
Incubator 1 Incubator 2
Incubator 1 Incubator2 Incubator 3 Incubator 4

Set I0HTR1370 I1HTR1370 I2HTR1370 I3HTR1370
temperature to I0HTR2370 I1HTR2370 I2HTR2370 I3vTR2370
37°C
Set I0HTR1250 I1HTR1250 I2HTR1250 I3HTR1250
temperature to I0HTR2250 I1HTR2250 I2HTR2250 I3HTR2250
25°C
After having performed operations from 1. to 14. for each of 4 incubators :

• remove IVT from QUATTRO instrument and extract IVT from plate
support,
• click on “Close” to exit from “Manual Control.
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C. Measurement recovering
• Connect the IVT to the PC with RS232 serial cable (COM4).
• Start the Logger Software.
• Select “Logger” and click on “Readout”.
IMPORTANT! Do not click “OK” immediately as following message is displayed.
• Unplug the RS232 serial cable from the IVT.

• Click “OK”.
• Save the record file in the folder you need.
• Temperature registered profile is displayed with date of test and serial
number of IVT used.
D. Switch off IVT
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E. Acceptance criteria
Measured temperature must be inside 37°C +/- 2°C after a stabilization time of 20
minutes.
37 °C 37 +/- 2°C
20 min
For a sequence of 4 incubators :
If criteria are not met, replace the defective incubator.
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Burn-in
2.5.11 Qualification results

After having performed all the verification tests, check the Excel datasheet
"Dprog_Vxx.xls" and more particularly the "Results" Sheet.
NO RED CELLS should be obtained (Green "Pass" results).
2.6 BURN-IN
The burn-in (Cycle Test-2) is a tool in case of mechanical failure (see page 2-16).
Prerequisite:
Wash bottle A : 400 ml distilled water
Reagent bottle: 20 ml distilled water (note : do not fill bottles above
collar)
Reagent bottle: 10 ml low density (note : do not fill bottles above
collar)
Controls: 1 ml low density (note : do not fill bottles above the collar)
Deep Well Plate or deep well dilution strips
Reagent Tips
Full box of sample Tips
8 sample tubes filled with 2 ml low density
To check the Burn-in, run the Cycle Test-2 protocol:

• Set the deepwell dilution plate or deepwell dilution strips (see page 2-16).
• Select: Add Assays using a new batch of samples then press OK.
• Select: New plate -> Select the D4_UK_Cycle Test-2_IP_01.asy in the
list and press the Open button.
• When the Load Sample Rack screen appears, load the 8 samples (low
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Burn-in
• When the test is complete, click the red square button to end the
test.
• The assay must complete without mechanical error. Save the data file.
Choose “Save Data as” under the “File” menu.
• Remove the used microplate.
Should a failure occur during the run, fix the corresponding module.
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ESP setup
2.7 ESP SETUP
Prerequisite:
• 75 patient samples – not clotted and not foamy
With the instrument connected select “Tools” and “Edit ESP
Detection Database”.
From the ESP detection database window select

“Run New Test …”.
Enter the fluid name: serum plasma
Enter volume in µl
Dispense / Aspirate profile : select

always 4
Enter the number of samples :

value 75. The first tube to define the
gain is not included.
Select the dispense location :

The aspirated sample will be
The first tube is used to define the dispensed into a microplate.
gain.
The first sample will be aspirated and
dispensed 5 times into the select Select sample caddy definition
waste bottle (reagent or control
bottle).
The Fluid Name must be identical for all volumes to be able to run a worklist
containing assays with different sample volumes. We recommend to use the name :
serum plasma
When all values are entered, press OK.

The system will prompt you to load the samples.
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ESP setup
The system will then prompt you to load the Sample Tips and
microplate to run the test.
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ESP setup
To set the gain of the sensor (dispense gain / aspirate gain) for a specific volume,
the system will aspirate and dispense fluid from the first test sample. 5
measurements will be performed starting with a maximum gain of 256. When no
curve can be defined (ADC out of range), the next measurement will be done with
half of the previous gain (128, 64, 32, 16). After 5 measurements the gain for the
aspirate step and the dispense step has been defined. This gain will be modified
(increased or decreased a little bit) so that the curve values are in a specific
range. When after aspirating no curve can be obtained, the sample will not be
dispensed. This can occur when the gain is too high e.g. with first sample.
The system will then aspirate and dispense the required test volume to the
microplate.
When all samples are dispensed into the microplate, the user has
the possibility to mark some samples as invalid. Select the sample
and press the “Mark As Invalid” button. The results of these invalid
samples will be skipped to define the ESP curve.
Press the “Close” button to continue. The following message

appears.
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ESP setup
Press the “OK” button. The pipettor arm will be initialized and the
results will be viewed.
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ESP setup
Press the “OK” button.
Set the correct Detection Threshold. As default this value is set to

0.01. The Threshold value can be changed by pressing the “Edit
Threshold” button.
We recommend to use the following Threshold settings:

• For volumes ≥ 30 µl : 0.001
• For volumes < 30 µl : 0.01 (less sensitive to avoid false clot errors)
Repeat this procedure for 10, 30 and 50 µl at least (some volumes
could be required by the customer).
When pressing the “Close” button you will be asked to save the
results (if not done). (the “Save” button can also be used)
Once all required dispense volumes are set up you can select in the
assay that you wish to use the ESP detection database and select
from the drop down box the correct curve.
The ESP database “ESPdetection.DB” is present in the folder
C: \ Program Files \ bioMérieux \ DAVINCI QUATTRO Program. However, to view
the ESP data, the instrument needs to be connected to the computer and the
instrument must be initialized.
Can we copy the ESP database from one instrument to another ? No, it is not
recommended because the volume curves are system related. Differences in tubing,
pressure sensor, pipettor assy, might have influence on the curve. So, each
instrument needs to be taught during installation.
WARNING! DEACTIVATE THE ENGINEERING MODE BEFORE ENDING THE

INSTALLATION;
To do so, remove the "/eng" from the shortcut.
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LIS-LINK configuration set up (optional)
2.8 LIS-LINK CONFIGURATION SET UP (OPTIONAL)
• System Options
Data Path : C:\ Document Settings \ User \ My Document

(directory where Revelation DSX places result data for the LIS-Link application)
Machine Name : We recommend using the DAVINCI QUATTRO Serial Number.

(It is not required for communication. This is the instrument identifier included in the
ASTM Header record.)
Local Computer Name : If using TCP / IP, this is the computer name on the LAN
(This is the LIS identifier included in the ASTM Header record.)
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• Communications
Select Serial settings as required by host

(If using TCP / IP, enter a Port Number and the LIS Host Name. We recommend
using the default port 13000.)
• Customized Defaults
Leave - Results are not automatically tagged for transmission to the LIS.
Send - Results are automatically tagged for transmission to the LIS.
The usual situation is to send results to the LIS. If “Leave” is selected, the user will
have to manually flag each individual record for transmission. By using “Send” as
the default, all results are pre-flagged and only the results that will not be sent have
to be manually selected. It’s normally less work to set this selection to “Send”.
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• Aborted Assays
Read Notes. Self-explanatory.

If sample IDs have been read or test orders input to the LIS database, what should
be done with that data if the assay is aborted ? (Delete - Run again - Leave)
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• Query Style
Query Mode, for ALL available Sample Ids at one time.

This is the query that is sent to the LIS Host
H|\^&|||QUATTRO||||||||1|20040408145049
Q|1|ALL||||||||||O
L|1|N
Query Mode, for Sample IDs scanned.

This is the query that is sent to the LIS Host
H|\^&|||QUATTRO||||||||1|20070410164827
Q|1|0008182||||||||||O
Q|2|0009830||||||||||O
Q|3|0009857||||||||||O
L|1|N
0008182, 0009830 and 0009857 are the scanned Sample IDs.
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• Name Mapping
1. Enter assay name used by Host (e.g. HIV) in “LIS Name” box.
2. Enter assay name used by QUATTRO (e.g. HIVAgAb.asy).
It’s important to include the asy extension.
3. Select the results you want transmitted to the Host
For each selection a result record will be sent to HOST.
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• Vendor
Two DLL files were installed with LIS-Link. They are located in :
C:\ Program Files \ Common Files \ Dynex Technologies \ LIS-Link
− Vendor1.dll
− defaultven.dll (= ASTM)
From the list of available Vendors, select the one you want (normally use
defaultven.dll), and click the green “Set Active” checkmark.
Default Protocol (Deaultven.dll)

H|\^&|||QUATTRO||||||||1|20040408145049|
P|1||dsx123||Peter
O|1|dsx123||^^^hiv
O|2|dsx123||^^^hbsag The Default Protocol Vendor requires the same
Sample ID number in the Patient and Order
P|2||dsx12345678||Henry
records.
O|1|dsx12345678||^^^hiv The Default Protocol uses Manufacturing
O|2|dsx12345678||^^^hcv records to report errors.
L|1|N
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Enabling the LIS communication in THE DAVINCI QUATTRO software (optional)
2.9 ENABLING THE LIS COMMUNICATION IN THE

DAVINCI QUATTRO SOFTWARE (OPTIONAL)
• Start the system.

• Start the DAVINCI QUATTRO Software.
• In the main menu, select "Tools", "Configure system".
The "Setup DAVINCI QUATTRO" window is displayed.

• Select the "LIS" tab.
• Tick the "Enable LIS support" checkbox.
• Select appropriate options in the "Reprocessing Options" area.
• Click on "OK".
.
The default setup is shown in the example below.
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Enabling the LIS communication in THE DAVINCI QUATTRO software (optional)
DAVINCI QUATTRO Installation Checklist
Item Done Printout

Physical installation of the instrument -
Install DAVINCI QUATTRO Software v 6.10a -
1 Check / Setup the Date & Time and Time properties and the -
printer page size
2 Startup the instrument & Print the Self Test Y
3 Language selection (optional) -
4 Print the workspace calibration data and the module Y
coordinates
5 User administration (optional) -
6 Setup Laboratory Information to appear at the top of the result -
reports
7 Setup the Sample Identification Module -
8 Install the assay protocols (depending on the customer needs) -
9 Setup the correct sample tubes and caddies -
Mechanical tests
10 Set the deepwell dilution plate or deepwell dilution strips -
11 Run the Tip Alignment Test -
12 Run the Sample Rack Pickup & Vertical Barcode Scanner Test -
13 Run the Plate Movement Test -
14 Run the Horizontal Barcode Scanner Test -
15 Dprog manual entry Y
16 Reader Verification Y
17 Calibration plate -
18 Pipette volume calibration -
19 High volume Air Gap check -
20 Pipettor Verification Y
21 Wash head offsets -
22 Washer Pump calibration A, B, C, D -
23 Washer Verification Y
24 Incubator verification Y
25 Burn-in Y
26 ESP setup -
27 Engineering mode (close the service) -
Notes / Comments: ……………………………………………………………………………..
……………………………………………………………………………..
Performed by _____________ Date _________ Instrument Serial Number _________
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3 S O F T WA R E I T E M S
3.1 WINDOWS XP LOGON
After switching on the computer, a logon screen appears.

As default 3 accounts are available :
• Super -password: bmxall (administrator rights) ONLY FOR SERVICE

ENGINEER
• Labman - password: labman(administrator rights)
• User -password: user (user rights)
NOTE: To install software, you have to be logged on with administrator

rights.
3.2 DAVINCI QUATTRO LOG ON
• Super ONLY FOR SERVICE ENGINEER

Password similar to Windows
• Admin (administrator rights)
• User (user rights)
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Software Items
File structure
3.3 FILE STRUCTURE
The QUATTRO software can be found in the folder :

C: \ Program Files \ bioMerieux \ DAVINCI QUATTRO Program
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Software Items
File structure
Software directories
Assays : this folder contains the assay files

AutoErrorRecovery : this folder contains 1 file ; default.aer (the error recovery
steps).
bioMerieux Text : contains the MECS PE export files when the function is
enabled (in this case no dat file will be generated in the Plates folder)
Curves : contains the saved assay curves
Levey Jennings : the Levey Jennings file
Logs : log files - each time the software is started a new log file is created
Plates : dat files containing the OD values (encrypted file - data editor to edit the
files)
Plates ID Cycles : text files containing the scanned plate ID’s
Report : pdf files containing the plates and workspace layout
Sample ID Cycles : text files containing the scanned sample ID’s
Selftests : contains the saved selftest files (e.g. Test1.tst)
SID : sid files containing the sample ID’s of the run
Sounds : sound files used for errors, warnings, …
Text : the result reports in Ascii format (txt - txp) when export function is enabled.
WFV : Verify Read data files with the OD values (Ascii format)
Worklists : the saved templates (wlt) create buttons e.g. Washer Clean,
Worklist HIV
The software creates two files when a software crash occurs. In this context, a crash
refers only to the situation where WindowsXP shuts down the Quattro software.
These files contain technical details of the crash and record the state of the
computer when the crash occurred. When returned to Dynex, software engineers
can examine these files using specialized debugging software and other utilities that
will help identify the line of code where the condition that caused the crash can often
be identified. These files are highly technical, and casual examination of these files
will probably not be useful to the support technician.
revel_crashXXX.dmp and revel_crashXXX.log
The XXX represents a number 001, 002, 003, …, etc. The numbers begin
automatically with 001 and increment as files accumulate. The Quattro software will
continue to accumulate these files until they are deleted manually. Each crash event
will produce a pair of files with the same number designation. After the system has
been in use awhile, it is not unusual to see an accumulation of 5-10 pairs of these
files. The service engineer may routinely clear these files if desired. They will not
cause problems if they remain.
Usually, only the last pair of files is necessary. You may check the creation date of
the files and select the pair that was created when the specific crash event occurred.
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Software Items
Database
3.4 DATABASE
The DAVINCI QUATTRO software is using a local SQL Server called

DYNEXLOCAL.
All bottle data, tube data, rack data, fluid data, passwords and user settings are
stored in the database. The patient results are not stored in the database.
This Microsoft SQL Server 2000 Desktop Engine (MSDE 2000) supports several
databases. (SQL = Structured Query Language)
The SQL Server 2000 database is using 2 types of files (secondary data files are not
used):
• Primary data files :
The primary data file is the starting point of the database and points to the
other files in the database. Every database has one primary data file. The
recommended file name extension for primary data files is .mdf.
• Log files :
Log files hold all of the log information used to recover the database.
There must be at least one log file for each database, although there can
be more than one.
The recommended file name extension for log files is .ldf.
During the software installation you will be requested to install the database
software.
The 4 system databases will be installed in the folder C:\ Program Files \ Common
Files \
Dynex Technologies \ Databases \ MSQL$DYNEXLOCAL \ Data. The master
database contains all the default settings for tips, plates, bottles, … and also the
default login account “admin”.
• master.mdf
mastlog.ldf
• model.mdf
modellog.ldf
• msdbdata.mdf
msdblog.ldf
• tempdb.mdf
templog.ldf
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Software Items
Database
• master
The master database records all of the system level information for a SQL Server system.
It records all login accounts and all system configuration settings. master is the database that
records the existence of all other databases, including the location of the database files.
master records the initialization information for SQL Server.
• tempdb
tempdb holds all temporary tables and temporary stored procedures. It also fills any other
temporary storage needs such as work tables generated by SQL Server. tempdb is a global
resource; the temporary tables and stored procedures for all users connected to the system are
stored there. tempdb is re-created every time SQL Server is started so the system starts with a
clean copy of the database. Because temporary tables and stored procedures are dropped
automatically on disconnect, and no connections are active when the system is shut down,
there is never anything in tempdb to be saved from one session of SQL Server to another.
By default, tempdb autogrows as needed while SQL Server is running. Unlike other databases,
however, it is reset to its initial size each time the database engine is started.
• model
The model database is used as the template for all databases created on a system. When a
CREATE DATABASE statement is issued, the first part of the database is created by copying in
the contents of the model database, then the remainder of the new database is filled with
empty pages. Because tempdb is created every time SQL Server is started, the model
database must always exist on a SQL Server system.
• msdb
The msdb database is used by SQL Server Agent for scheduling alerts and jobs, and recording
operators.
The first time the QUATTRO software is started, a new database

(RevelationDSX_Data.mdf) will be created. The template is copied from the model
database. When an assay is opened or started, the specific assay settings are
saved into this new database file.
• RevelationDSX_Data.mdf
RevelationDSX_Log.ldf
This RevelationDSX database contains several tables. Each table contains one
specific setting:
• Tips Database (only viewable in engineering mode)
• Bottles Database (only viewable in engineering mode)
• Barcode Script Database (only viewable in engineering mode)
• Tubes Database
• Plates Database
• Pipette Fluid Database
• Washer Fluids Database
• Dilutions Database
• Sample Racks Database
New created users with password are also saved into the RevelationDSX_Data.mdf
database file.
®
Software Items
Database
The default settings are managed by the startup code of the QUATTRO software.
Every time the software starts, the default settings are rewritten to the database.
You are able to delete the default settings. But, when restarting the software the
default settings are reloaded.
NOTE: By removing the DAVINCI QUATTRO software or Microsoft SQL
Server Desktop engine both files ; RevelationDSX_Data.mdf and
RevelationDSX_Log.ldf will not be deleted. This database can be
deleted (e.g. when user names and passwords are lost) by following
this procedure :
1. The database service has to be stopped first before you can delete the 2
files.
Select Start - Settings - Control Panel - Administrative Tools - Services -
MSSQL$DYNEXLOCAL. Press the “Stop” button to stop this service.
The service status will change from ‘started’ to ‘stopped’.
or
The database services can also be stopped by selecting the icon in the right
bottom corner and selecting the “Stop” button.
2. Delete both files ; RevelationDSX_Data.mdf and RevelationDSX_Log.ldf in

the folder
C:\ Program Files \ Common Files \ Dynex Technologies \ Databases \
MSQL$DYNEXLOCAL \ Data
Next time when the QUATTRO software is started, the two files will be automatically
generated and the database is filled with the default settings from the model
database. All the settings previous loaded by the installation of the assays and the
previous created users / passwords are deleted. The login screen does no longer
appear until new users are created. By opening or starting an assay the new
database will be refilled with the specific settings.
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Software Items
Database
Tips database (only accessible in engineering mode)
− Reagent tip
− Sample tip
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Software Items
Database
Bottles database (only accessible in engineering mode)
− Reagent bottle
− Reagent bottle 200µl (Tip submersion 4.5 mm for dispensing volumes
between 101 – 200µl)
− bioMérieux control bottle
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Software Items
Database
Dead volume bioMérieux control bottle

For the bottle definition in the application software there are three possibilities for the
bottom shape: Flat, U Shape and V Shape. For the bMx control bottle the most
suitable bottom shape will be a flat one. By use of Internal Diameter, Detect Stop
Height and Tip Submersion the application software calculates the dead volume of
the bioMérieux control bottle (visible during loading).
hdv = det stop + tip sub (1)
where det stop = detect stop height (= 2 mm) and tip sub = tip submersion (= 1 mm)
Tip submersion
of 2 mm
Detect Stop
Dead Height of 1 mm
volume
Bottom bottle
Thus, the height of the dead volume is 3 mm and the Internal Diameter is 14 mm.
The calculated dead volume will be then:
Vdead = π ⋅ r 2 ⋅ hdv = π ⋅ 7 2 ⋅ 3 ≈ 461.81 µl (2)
where hdv = height of dead volume and r = bottle internal radius.
Since the bioMérieux control bottle has a small kick, the actual dead volume will be
larger than what is calculated by the application software.
When the software instructs to load control fluid during the load process of an assay
protocol,
it asks to load the required control fluid for the assay + the calculated dead volume
of the bioMérieux control bottle. The required control fluid for the assay consists of
the programmed target volume in the assay protocol + the extra fluid that is asked to
the pipette module + some extra fluid to be absolutely on the safe side (13/14 µl).
Therefore, enough fluid will be present in the bioMérieux control bottle if the volume
is loaded that is asked by the application software.
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Software Items
Database
Actual volume that can be aspirated from a bMx control bottle
The height of the bottle where the vertical wall is not tapered is :
h⊥ = h 2 − t − S 2 = 32.0 − 0.7 − 0.7 = 30.6 mm (3)
where h2 is the height of the bottle where the vertical wall is not tapered including
space between bottom and table and bottom thickness, t is the space between
bottom and table and S2 is the bottom thickness. Instead of 29 mm the typical
value for h2 was used, which is 32 mm.
According to the first equation the height of the dead volume is 3 mm. Therefore, the
height of the liquid column that can be aspirated is :
hasp = h⊥ − hdv = 30.6 − 3 = 27.6 mm (4)
where h⊥ = the height of bottle where the vertical wall is not tapered
hdv = the height of the dead volume
The actual volume that can be aspirated with proper liquid level tracking will be then
:
Vasp = π ⋅ r 2 ⋅ hasp = π ⋅ 7 2 ⋅ 27.6 ≈ 4249 µl (5)
where r = bottle internal radius and hasp = height of liquid column that can be
aspirated
Maximum load volume bMx control bottle

The Neck Height is the height from the top of the bottle where no fluid is
allowed. When the pipette module detects the liquid level in this section an
error message is generated. Then the operator should remove the control
fluid from the neck prior to continue.
The Neck Height has been set to 12 mm (h1 - h2). where h1 is the total height
of the bottle and h2 is the height of the bottle where the vertical wall is not
tapered including space between bottom and table and bottom thickness.
Instead of 29 mm the typical value for h2 was used, which is 32 mm.
The Maximum load volume can be calculated by use of the outcome of
equation 2, which results in 4711 µl. Therefore the Maximum load volume has
been set at 4710 µl in the Define Bottle dialog box.
®
Software Items
Database
1. Bottle height
2. Internal diameter
3. External diameter
4. Neck height 4
5. Detect stop height + Tip submersion
6. Maximum Load Volume
7. Bottom Thickness
6
2
1
Dual detect
The option Do Dual Detect has been enabled in the Runtime Options.
Dual detect divides the level detection phase into 2 zones, the upper zone and the
lower zone. Dual detect was created for users who use more than 50% of the
volume in the tube. The reason this was done is because the system is a little faster
if it does not need to pull the syringe plunger all the way up to detect liquid in the
upper zone. In case the liquid is accidentally in the lower zone, the syringe will be
pulled up again
If you use less than 50%, you should leave this option unchecked. This is also
faster. The syringe is pulled up completely to perform the liquid detection in one
step. There is enough air inside the syringe to perform a liquid detection on an
almost empty tube.
Dual detect splits the level detection phase into 2 zones, the upper zone of a bottle
and the lower zone.
2383 = 1st Detect start 3032 = 2nd Detect start

3082 = 1st Detect stop 3782 = 2nd Detect stop
®
Software Items
Database
Generation of bubbles on samples, controls and reagents

It’s important that the samples, the controls and the reagents are placed on the
instrument without bubbles or foam on the surface. However, we observed that
during processing DAVINCI QUATTRO generates bubbles in samples, controls and
reagents. This might cause lower dispense volumes in the wells, which can result in
incorrect test results.
1. Samples
Bubbles are generated during the liquid detection and the “kiss-off”.
The liquid detection is only performed the first time the sample is aspirated from the
tube. During the detection the system is blowing air into the sample liquid.
The “kiss-off” occurs when a used tip is called to make a new sample aspiration
(when dispensing samples in parallel mode, single shot – multiple shot is not
allowed for samples). It ejects any remaining liquid from inside the tip before
beginning the aspiration. The purpose ofthis operation is to prevent a small droplet
of liquid from flying up and contaminating the spigot. During this process the system
is again blowing air into the sample liquid.
Despite the generation of bubbles in the sample liquid, the R&D study showed that
as the original liquid level is correctly tracked, the tip does not dispense these
bubbles in the wells. Therefore, make sure that the settings of the used sample
tubes are correctly defined in the Tubes Database (Tools - Edit Databases - Tubes
Database) to avoid incorrect liquid tracking. Especially, the internal diameter of the
tube is very critical and needs special attention. A small deviation might be enough
to have incorrect liquid tracking. Most sample tubes are tapered. The lowest
measured internal diameter (mostly just above the U-part) must be used in the tubes
database.
NOTE: It is not possible to permanently modify the bioMérieux predefined
sample tubes and sample tube caddies. After restarting the database,
the original predefined settings will be given. In case one of the
predefined sample tube settings can’t be used, a new sample tube
and a new sample tube caddy have to be created.
2. Controls
Bubbles are generated during the liquid detection and the “kiss-off”.
The liquid detection is only performed the first time the control is aspirated. During
the detection the system is blowing air into the control liquid.
The “kiss-off” occurs when a used tip is called to make a new control aspiration. It
ejects any remaining liquid from inside the tip before beginning the aspiration. The
purpose of this operation is to prevent a small droplet of liquid from flying up and
contaminating the spigot. During this process the system is again blowing air into
the control liquid.
To avoid the kiss-off we strongly recommend activating “Always use new tip:” for all
Controls in the Pipette Samples/Standards/Controls step of the assay protocols.
You have to instruct your customers, if the same controls are used in a next run, first
remove still existing bubbles.
®
Software Items
Database
3. Reagents
Because reagents are dispensed in multi-shot mode, the pipette will aspirate an
excess of fluid. It will immediately perform a “pre-shot” of the fluid back into the
bottle. It will then go on to dispense multiple aliquots as required, followed by a
“post-shot” of excess liquid back into the bottle. Both the pre- and post-shot
dispense can cause bubbles.
Some reagents with protein or detergents can produce quite a layer of foam that can
interfere with future liquid level detection operations. The bubbles may be seen as
the liquid level with the consequence of dispensing air.
Bubbles are also generated during the liquid detection.
To keep the pipette tip immersed in fluid when it is performing an aspiration, there is
a process in the software that can force a Liquid Level Detection to occur. The
accuracy of dispenses have been defined at 3.3 %. Each time it dispense an aliquot,
it adds 3.3% of that volume to the tally. When the accumulated total reaches a
volume that is 50 % of the fluid volume defined by the tip submersion depth, the
software will force another Liquid Level Detection to make sure the tip remains
submersed to the required depth.
To obtain a safe operation, we have to exclude more than 1 Liquid Level Detection
on the reagent bottle.
Resolution :
We have to avoid more than 1 reagent tip is used to dispense

reagent to the plate. By setting the tip submersion to 2.5 mm, only 1
tip will be used if the reagent volume is not larger than 100 µl.
NOTE: For assays using 200 µl diluent or conjugate (e.g. Chagatek, Ortho
HCV), a new reagent bottle “Reagent bottle 200 µl” with a tip
submersion of 4.5 mm has been defined. The dead volume will
increase from 1.27 ml to 1.97 ml. For these reagents 1 ml extra
reagent must be loaded than indicated on the screen to avoid the
system is dispensing the last wells of the plate in single shot mode.
We also have to avoid the situation that a reagent container, filled
with diluent or conjugate, is used for more than 1 plate. When the
reagent is used for a second plate, a new tip is taken and a new
Liquid Level Detection will be performed.
• When programming assays, use unique names for the conjugate and the
diluent. This means that the same pipette fluid name may not be used in
another assay protocol. Most all conjugates are unique. But, for some
predilution assays, the diluent might be identical. For these assays, use
different names. Check the installed protocols.
®
Software Items
Database
• To be able to run an assay on more than 1 plate (e.g. 2 HIV plates), the
original assay protocol and the pipette fluids have to be copied to an A, B,
C & D version where protocol A is using diluent A and conjugate A, protocol
B is using diluent B and conjugate B, protocol C is using diluent C and
conjugate C and protocol D is using diluent D and conjugate D. In this way
each plate will use its own reagent bottle for diluent and conjugate.
The creation of different versions is not necessary when your customer is

not running more than 1 plate of the same assay simultaneously and it’s
also not necessary for assays, which are not having a diluent and
conjugate step.
Why only for diluent and conjugate ?

Diluent and conjugate are fluids, which cause more bubbles than TMB / UP or
sulphuric acid. Therefore, we consider this measure not required for TMB / UP and
sulphuric acid.
Displayed volume to load

DISPLAYED VOLUME = ( DISPENSED VOLUME (wells) + DEAD VOLUME ) x
1.033
WARNING! For processing predilution assays on DAVINCI QUATTRO, we

recommend to contact GCS. We can help you to find the correct
system settings.
®
Software Items
Database
Barcodes Scripts Database (only accessible in engineering mode)
®
Software Items
Database
Tubes Database
− 13x75 mm sample tube
®
Software Items
Database
Plates database
− 1 ml dilution plate
− 1 ml dilution strips
− Break-4
− Break-4 increased dispense height
(to be used for assays with accuspheres, the TP recombinant assay,
the HBsAg Ultra, a-HBc, …)
®
Software Items
Database
Define Washer Settings
Actual measurements are from a table that the plate is resting on (= 0) to the bottom
of the aspirate pins of the wash head.
These offset values can be used for all kind of microplates.
Top of well height

Default this value is 14.5 mm, which originates from the Height of the Plate.
However, after each dispensing the aspiration pins move to this height while they
are aspirating. An optimum value was found by increasing the top of the well height
by 1.6 mm resulting in a height of 16.10 mm.
Dispense height
Default this value is 1 mm above the Top of well height. An optimum value was
found by increasing the Dispense height by 0.6 mm resulting in a height of 16.10
mm.
Aspirate height
Default this value is 1 mm above the bottom of the well and is therefore defined by
Height in Plate Characteristics and Height in Well Characteristics of the Define Plate
dialog box. An optimum value was found by decreasing the Aspirate height by 0.7
mm resulting in a height of 3.90 mm, which equals to a distance of 0.3 mm above
the bottom of the wells.
®
Software Items
Database
Sweep height
Raises the aspiration pins on so that the aspiration pins can be moved back and
forth in the well while the fluid is being aspirated. Default this value is 2 mm above
the bottom of the well and is therefore defined by Height in Plate Characteristics and
Height in Well Characteristics of the Define Plate dialog box.
An optimum value was found by decreasing the Sweep height by 0.7 mm resulting
in a height of 4.90 mm, which equals to a distance of 1.3 mm above the bottom of
the wells
Allow bottom washing

Not used in our Elisa assays.
Sweep stroke
Specifies the amount of deflection the aspiration pins move from the center of the
well.
The well diameter of the Break-4 is 6.4 mm. By default this value is 3 mm.
An optimum value was found by decreasing the Sweep stroke to 2.5 mm.
Plates with accuspheres

A tip on the pipette module of DAVINCI QUATTRO always dispenses at a height
that is 3.5 mm above the target liquid level (registry key setting “MPdispheight = 56”
- 56 motor steps - 3.5 mm).
When there are accuspheres in the wells of the microplate and the pipette module
has to dispense small volumes the tip will be too close to the accuspheres, which
can cause splashing. Of the assays that are going to be run on DAVINCI QUATTRO
the following ones contain accuspheres:
− Vironostika HIV Uni-Form II plus O
− Vironostika HIV Uni-Form II Ag/Ab
These assays start with a 100 µl dispense step (sample / control or specimen
diluent).
The application software calculates the target liquid level and then adds 2 mm as
dispense height for the tip.
The application software assumes a cylindrically shaped well and therefore

calculates a target liquid level of 3.1 mm from the bottom of the well.
The tip then dispenses from a height of 3.1 mm + 3.5 mm = 6.6 mm.
In the accusphere Microplates of these assays, it has been described that the
maximum diameter of the accuspheres is 4.75 mm. It has been decided that the tip
on the pipette moduleshould dispense also at a height of 3.5 mm above the
accusphere, which is 8.25 mm above the bottom of the well. The maximum diameter
of the accusphere is 4.75 mm and this is 1.65 mm (4.75 - 3.1) larger than the liquid
level in the well for 100 µl. Therefore a plate file must be created with a well height
that is 1.65 mm ( 1.7 mm) smaller. This has been done in Well Characteristics of
the Define Plate dialog box.
®
Software Items
Database
When the imaginary height of the well has been decreased it is necessary that the
height of the well is still the same for the washer.
The software has automatically adjusted the Offset of Aspirate height and Sweep
height with 1.7 mm downwards to keep the Actual Aspirate height and Sweep height
respectively 3.90 mm and 4.90 mm. In this way the washer shall use the actual well
height and the pipette module the imaginary well height.
NOTE: It is necessary to create a new name for the second plate file
because else conflicts will occur in the system database of the
application software. When a validated bMx protocol has been run
without accuspheres and afterwards a validated bMx protocol with
accuspheres is loaded, the question appears on the screen if the
operator wants to use the plate file of the database version or the
imported version (protocol that is loaded). When the operator
chooses for the database version the accuspheres will get pierced !
2
8
5 4 1
7 6
1. Plate Height
2. Well Height
3. Internal Diameter
4. Top of Well Height (washer setting)
5. Dispense Height (washer setting)
6. Aspirate Height (washer setting)
7. Sweep Height (washer setting)
8. Sweep Stroke (washer setting)
®
Software Items
Database
Disposable tip
Well
4
3
2
1
1 Liquid level at 3.1 mm for 100 µl
2 Maximum diameter accusphere is 4.75 mm
3 Dispense height above target level is 3.5 mm
4 Dispense height above accusphere set at 3.5 mm
NOTE: For some assays e.g. HBsAg Ultra and TP you have to use the plate
setting with the increased dispense height to avoid carry-over while
dispensing the conjugate.
®
Software Items
Database
Pipette Fluids database
− Example Diluent
Meniscus offset for some reagents to correct the detected volume
Required reagent volume Meniscus offset

per well (µl)
25 0
50 150
100 500
>100 500 (maximum)
Washer Fluids Database
− WashBuf
®
Software Items
Database
Dilution Database
This database is empty. This is an example when adding a dilution step.
Note: Before defining a dilution, a diluent fluid must be present in the pipette fluid
database. If not, the software is crashing when adding a dilution step.
Therefore, an “Example Diluent” is always present in the database when
starting the software.
®
Software Items
Database
Sample Racks Database
− 13x75 mm sample tube caddy

®
Software Items
Dead volumes table
Users database
Default :
− Username : admin
− Password : no password
3.5 DEAD VOLUMES TABLE
Sample tube 13x75 mm : ± 160 µl

Sample tube 16x100 mm : ± 3 ml (tip length 75 mm)
Reagent bottle 1.27ml
Reagent bottle 200 µl 1.97ml
bMx control bottle : ± 470 µl (462 µl)
Wash buffer container : 270 ml
Deep Well Plate / Strips : between 50 and 75 µl
®
Software Items
Application software - software menus
3.6 APPLICATION SOFTWARE - SOFTWARE

MENUS
− Main menu (administrator logon) - Normal mode
®
Software Items
− Main menu (operator logon) - Normal mode
The operator with operator rights has no access to the following items :
− Edit User Access
− Edit Databases
− Edit ESP Detection Database
− Calibrate Pipette
− No access to assay programming screen, assays can not be created
and modified.
− Main menu (administrator rights) - Engineering mode
®
Software Items
− Assay Programming screen (only available with administrator logon) -

normal mode
®
Software Items
− Data screen
Available when the plate has been calculated or when opening a data file.
The options that are available depend on the assay protocol used to obtain the
results.
®
Software Items
− User Contacter
Double-click the DAVINCI QUATTRO Program User Contacter icon in the windows
status bar.
You can edit the settings by selecting the available message and by clicking “Edit
Contact Details”.
You can select Visual Alarm - Audible Alarm - Email Message. As default visual
alarm and audible alarm has been enabled and email message has been disabled
for all events.
By selecting the alarm type you can set :
− for the visual alarm : the foreground / background color
− for the audible alarm : the sound type and the repeat cycle
®
Software Items
Cleaning up the hard disk
3.7 CLEANING UP THE HARD DISK
It is recommended to delete the log files and the "bioMérieux Text" files during
preventive maintenance.
Folder: "Log" and "bioMérieux Text".
3.8 BACKUP
On regular base the customer can make a backup of the patient results. The OD
results are stored in the folder C:\ Program Files \ bioMérieux \ DAVINCI QUATTRO
Program \ Plates.
The Sample ID’s are stored in the folder C:\ Program Files \ bioMérieux \ DAVINCI
QUATTRO Program \ SID.
The dat files and the sid files can be copied to a CD or DVD. This can be done from
the windows explore screen by dragging all the files to the CD or DVD.
3.9 LOG FILE
The communication between computer and modules will be logged in a file. When
DAVINCI QUATTRO Software is started a new log file will be created storing all
these commands. The log file is saved in the folder : C:\ Program Files \ bioMérieux
\ DAVINCI QUATTRO Program \ Logs.
This file can be useful to investigate a hardware problem. The description of the
commands can be found in the manual “Appendix Manual Control Codes”. However,
this manual is not up-to-date. Some commands are missing.
Log files can be opened in notepad or workpad. Another possibility is to use the
viewer ViewLog.exe. With the viewer you can select between “view all events” or
“exclude commands”.
The viewer can be found in the folder C:\ Program Files \ bioMérieux \ DAVINCI
QUATTRO Program.
Example : (initialization of the instrument)
®
Software Items
Log File
4 16:46:22 7-9-2008 DAVINCI QUATTRO Program Version 6.10a started.

4 16:46:22 7-9-2008 Running on Microsoft Windows XP Professional Service Pack 2 (Build 2600)
8000 16:46:25 7-9-2008 User logged in: admin
2 16:46:25 >> "C0STS" (Timeout = 20, ID = 128)
2 16:46:25 << "OKY" (ID = 128)
2 16:46:25 >> "C0VER" (Timeout = 20, ID = 129)
2 16:46:25 << "VERCM 1.01" (ID = 129)
2 16:46:25 >> "C0SER" (Timeout = 20, ID = 130)
2 16:46:25 << "SER1EPA2162" (ID = 130)
2 16:46:25 >> "C0MAP" (Timeout = 30, ID = 131)
2 16:46:25 << "MAP D0S0I1I3A0W0I2I0 P0X0 " (ID = 131)
2 16:46:25 >> "P0SER" (Timeout = 20, ID = 132)
2 16:46:26 << "SER1PMB2277" (ID = 132)
2 16:46:26 >> "C0GPS" (Timeout = 20, ID = 133)
2 16:46:26 << "GPS 00" (ID = 133)
2 16:46:26 >> "X0LCK1" (Timeout = 20, ID = 134)
2 16:46:26 << "LCK1" (ID = 134)
2 16:46:26 >> "X0SMHYY" (Timeout = 20, ID = 135)
2 16:46:26 << "SMHYY" (ID = 135)
2 16:46:26 >> "P0SMHYXX" (Timeout = 20, ID = 136)
2 16:46:26 << "SMHYYY" (ID = 136)
2 16:46:26 >> "W0SER" (Timeout = 25, ID = 137)
2 16:46:26 >> "A0GSC" (Timeout = 20, ID = 138)
2 16:46:26 << "GSC0000" (ID = 138)
2 16:46:26 >> "A0SER" (Timeout = 25, ID = 139)
2 16:46:26 >> "I0SER" (Timeout = 25, ID = 140)
2 16:46:26 >> "I1SER" (Timeout = 25, ID = 141)
2 16:46:26 >> "I2SER" (Timeout = 25, ID = 142)
2 16:46:26 >> "I3SER" (Timeout = 25, ID = 143)
2 16:46:26 >> "S0GSC" (Timeout = 20, ID = 144)
2 16:46:26 << "GSC00000" (ID = 144)
2 16:46:26 >> "S0SER" (Timeout = 25, ID = 145)
2 16:46:26 >> "P0SER" (Timeout = 25, ID = 146)
2 16:46:26 << "SER1WMB2184" (ID = 137)
2 16:46:26 << "SER1AXA2197 " (ID = 139)
2 16:46:26 << "SER1IMA4114 " (ID = 140)
2 16:46:26 << "SER1IMA4115 " (ID = 141)
2 16:46:26 << "SER1IMA4210 " (ID = 142)
2 16:46:26 << "SER1IMA4112 " (ID = 143)
2 16:46:26 >> "W0VER" (Timeout = 25, ID = 147)
2 16:46:26 >> "A0VER" (Timeout = 25, ID = 148)
2 16:46:26 >> "I0VER" (Timeout = 25, ID = 149)
2 16:46:26 >> "I1VER" (Timeout = 25, ID = 150)
2 16:46:26 >> "I2VER" (Timeout = 25, ID = 151)
2 16:46:26 >> "I3VER" (Timeout = 25, ID = 152)
2 6:46:27 << "SER1PMB2277" (ID = 146)
®
Software Items
Log File
2 16:46:27 << "SER1SMF1799" (ID = 145)

2 16:46:27 << "VERWM1.15" (ID = 147)
2 16:46:27 >> "P0VER" (Timeout = 25, ID = 153)
2 16:46:27 << "VERAM v1.21" (ID = 148)
2 16:46:27 >> "S0VER" (Timeout = 25, ID = 154)
2 16:46:27 >> "W0STS" (Timeout = 90, ID = 155)
2 16:46:27 >> "A0DSX" (Timeout = 20, ID = 156)
2 16:46:27 << "VERIM v2.06" (ID = 149)
2 16:46:27 << "VERIM v2.06" (ID = 150)
2 16:46:27 >> "I0STSa" (Timeout = 25, ID = 157)
2 16:46:27 >> "I1STSa" (Timeout = 25, ID = 158)
2 16:46:27 << "VERIM v2.06" (ID = 151)
2 16:46:27 << "VERIM v2.06" (ID = 152)
2 16:46:27 >> "I2STSa" (Timeout = 25, ID = 159)
2 16:46:27 >> "I3STSa" (Timeout = 25, ID = 160)
2 16:46:27 << "VERPM 1.05" (ID = 153)
2 16:46:27 << "VERSM 2.20" (ID = 154)
2 16:46:27 << "DSXe" (ID = 156)
2 16:46:27 >> "P0GER" (Timeout = 20, ID = 161)
2 16:46:27 >> "S0GRS" (Timeout = 20, ID = 162)
2 16:46:27 >> "A0STSa" (Timeout = 120, ID = 163)
2 16:46:27 << "GER 255" (ID = 161)
2 16:46:27 << "GRSN" (ID = 162)
2 16:46:27 >> "P0GRS" (Timeout = 20, ID = 164)
2 16:46:27 >> "S0STS" (Timeout = 30, ID = 165)
2 16:46:27 << "GRSN" (ID = 164)
2 16:46:27 >> "P0SHRZ040008000015" (Timeout = 20, ID = 166)
2 16:46:27 << "SHRZ040008000015" (ID = 166)
2 16:46:27 >> "P0SRPZ040030000030S" (Timeout = 20, ID = 167)
2 16:46:27 << "SRPZ040030000030S" (ID = 167)
2 16:46:28 >> "P0SRPZ040070000065T" (Timeout = 20, ID = 168)
2 16:46:28 << "SRPZ040070000065T" (ID = 168)
2 16:46:28 << "OKY" (ID = 155)
2 16:46:28 >> "P0SRPE024620000030" (Timeout = 20, ID = 169)
2 16:46:28 >> "W0SHS" (Timeout = 20, ID = 170)
2 16:46:28 << "SRPE024620000030" (ID = 169)
2 16:46:28 << "SHS0108" (ID = 170)
2 16:46:28 >> "P0STS" (Timeout = 45, ID = 171)
2 16:46:28 >> "W0SAS" (Timeout = 20, ID = 172)
2 16:46:28 << "SAS400" (ID = 172)
2 16:46:28 >> "W0SDP4" (Timeout = 20, ID = 173)
2 16:46:28 << "SDP4" (ID = 173)
2 16:46:28 << "OKY" (ID = 165)
2 16:46:28 >> "W0SPPA008" (Timeout = 20, ID = 174)
2 16:46:28 >> "S0GVB" (Timeout = 5, ID = 175)
2 16:46:28 << "SPP190 048 066" (ID = 174)
2 16:46:28 >> "W0SPPA016" (Timeout = 20, ID = 176)
®
Software Items
Auto Error Recovery
3.10 AUTO ERROR RECOVERY
The folder C: \ Program Files \ bioMerieux \ DAVINCI QUATTRO Program \

AutoErrorRecovery contains the file default.aer. This file contains the steps the
system has to be executed when an error occurs.
Some examples :
[GetTip.PPickUpTip] In case a tip is not present, the system will

try to pickup another tip.
ERRNTN=TryAnotherTip (repeat : maximum 3 times)
[RwellFillVerify] In case the well fill verification failed, the
system will continue the run.
WellFillVerify=AbortWellFillVerifyFailedWells
[Retry2 GetTip.PPickUpTip] after 3 times the system will try again 3
times to pickup a tip
ERRNTN=TryAnotherTip
[ERRNTN=TryAnotherTip after 6 times the system will try again
3 times to pickup a tip.
Retry3 GetTip.PPickUpTip]
[PDetectFluidSurface] when no liquid can be detected, the system
will try again with a new tip
ERRDEN=DetectAgainWithNewTip
Procedure to generate more auto recovery programs
− When the error occurred, select the checkbox “Learn error recovery”.
®
Software Items
Auto Error Recovery
− Press the “Recovery Options >>” button.

− Select the correct step(s) the system has to be performed to solve the
problem.
− Press the “More Generally” or “More Specifically” button and then press
the “Learn” button.
This new learning process will be added to the default.aer file.
WARNING! We strongly recommend not adding extra auto error recovery

steps. To avoid wrong system operation, it’s better that the file is
not changed.
When new auto error recovery steps are needed, please contact
GCS.
®
Software Items
Auto Error Recovery
The default.aer file contains the following settings :
[PMoveZTo]
ERRSTR=SelftestPipette
[REjectPlate]
ERRP3=Retry
ERRP4=Retry
[Retry1 REjectPlate]
ERRP3=Retry
ERRP4=Retry
[Retry2 REjectPlate]
ERRP3=Retry
ERRP4=Retry
[IEjectPlate]
ERRP3=Retry
ERRP4=Retry
[Retry1 IEjectPlate]
ERRP3=Retry
ERRP4=Retry
[Retry2 IEjectPlate]
ERRP3=Retry
ERRP4=Retry
[PDetectFluidSurface]
ProcessTiterWells.GetSample.AspirateFromFluidHolder.ERRDEN=DetectAgainWithNewTip
PipetteSamplesToTiterWellsByOrder.ProcessTiterWells.GetSample.AspirateFromFluidHolde
r.ERRDEN=DetectAgainWithNewTip
[GetTip.PPickUpTip]
[Retry1 GetTip.PPickUpTip]
[RWellFillVerify]
WellFillVerify=AbortWellFillVerifyFailedWells
ERRNTN=TryAnotherTipRack
[Retry5 GetTip]
RunOutOfTips=LoadMoreTips
®
Software Items
Engineering mode
3.11 ENGINEERING MODE
To start the QUATTRO software in Engineering Mode, use the shortcut in the
"QUATTRO Tools" folder available on the Windows desktop ("super" account).
IMPORTANT! Always close this mode WHEN ENDING THE SERVICE.

The engineering mode is used to install the instrument and gives extra option:
Workspace calibration data and module coordinates can be printed or

saved to a file
(see below) => “Print” button available.
NOTE: The report can also be saved as RTF file. This file can be opened in
wordpad.
Access to Tip and Bottle database.
Extra shortcuts in the folder Tools : Plate cycle test, Tips alignment
test, Workspace calibration , Result settings, Print settings, User name
& Serial number, View last test results.
Printout Workspace calibration and module coordinates
bioMérieux Test Results
Font, size and title

Date : Thursday, September 15, 2005 can be edited with
“Result settings”
User Name : Marc
Product : DAVINCI QUATTRO
Can be edited with “User name & Serial
Product Serial Number : 1DXB0819
number”
Workspace calibration data

Workspace home offsets;
X home offset : 220 (13.2 mm)
Y home offset : 74 (4.6 mm)
Z home offset : 114 (7.1 mm)
Workspace pillar positions;
®
Software Items
Engineering mode
Front left pillar

X = 49 (2.9 mm, Expected : 3.0 mm, error : 0.1 mm)
Y = 3525 (220.3 mm, Expected : 220.0 mm, error : 0.3 mm)
Z = 3629 (226.8 mm, Expected : 227.0 mm, error : 0.2 mm)
Back right pillar;

Drawer calibration hole location;

Module plate coordinate calibration data;

AM Reader
Serial number : 1AXA1900
X = 1680, Y = 1360, Z = 290
Front left drawer

Serial number : 1ADA1857
X = 6520, Y = 3695, Z = 3570
Front right drawer

X = 8980, Y = 3685, Z = 3575
Back left drawer

X = 6525, Y = 1525, Z = 3570
Back right drawer

X = 8985, Y = 1525, Z = 3570
Washer
Serial number : 1WMA1867
X = 2125, Y = 65, Z = 3280
Incubator 1
Serial number : 1IMA3395
X = 6105, Y = 1350, Z = 2210
Incubator 2
X = 11455, Y = 1355, Z = 2225
Incubator 3
X = 6110, Y = 1340, Z = 260
Incubator 4
X = 11450, Y = 1355, Z = 255
®
Software Items
Some other functions
3.12 SOME OTHER FUNCTIONS
3.12.1 Data editor

To edit the patient data (OD values) the data editor can be activated. However, this
function may only be used in special controlled circumstances. By default this
function is not activated.
WARNING! The Data editor can be activated in special circumstances. It is a

tool created to check the data reduction of NON-BIOMERIEUX
protocols.
Plate reports generated with Data Editor activated are "flagged"
==> Manuall edited wells: ...".
To activate the data editor,
• log as "super".
The data editor MUST BE deactivated before end of service!
On the Windows desktop,

• click on the "QUATTRO Tools" folder.
This folder contains 2 shortcuts:
− to activate.
and
− to deactivate.
®
Software Items
How to use the data editor ?
A plate must be loaded in the DAVINCI QUATTRO software in order to activate the
‘Data’ menu item with the Data Editor present. Select from the DAVINCI QUATTRO
program File - Open - Plates.
Select a plate file. From the Data Folder, select Data Editor.
Manually edited wells” will be indicated. A new plate file will be created
“xxxxx.edited.dat”.
®
Software Items
3.12.2 Setup the Timeline Settings
Setup the Runtime Options
Ensure the following settings are present.

Dilution plate:
• 1 ml dilution plate
(deep well dilution plate)
• dilution strip plate
(deep well dilution strips)
The dispense and aspirate

pipette profile has to be set
to 4.
Low value : low speed

High value : high speed
With Optimize Timeline enabled, the software will use a different algorithm and try to
reorder scheduled plates and pipetting steps such that the total runtime of a worklist
is reduced. It’s used when some plates have already been scheduled and new
plates are scheduled. The software will try to reorder the previous scheduled plates.
If unchecked, this optimization is not done.
®
Software Items
Dual detect divides the level detection phase into 2 zones, the upper zone of a bottle
and the lower zone. This allows taller bottles and tubes to be level detected at any
liquid level whether the bottle is full or near empty. It should always be left on as
turning it off will prevent low volumes from being detected in tall bottles and tubes.
If you use less than 50%, you should leave this option unchecked. This is also
faster.
The syringe is pulled up completely to perform the liquid detection in one step.
There is
enough air inside the syringe to perform a liquid detection on an almost empty tube.
Setup the Error Recovery
Edit Timeline Symbols
®
Software Items
3.12.3 Create shortcut buttons
For the QUATTRO Software 6.10, 3 shortcut buttons are installed.

These buttons are used to perform 3 maintenance assays.
To start a run from a button, the button is linked to a worklist file.
®
Software Items
To Create a new button
It might be useful to create extra buttons (maximum 8 buttons).
e.g. a button “Routine” containing the HIV + HBSAG + HCV assay
− From the main Program, select Tools - Options
(Ensure this option is enabled. The buttons remain invisible if no buttons are
defined.)
− Select the ‘Add’ button.

− In the screen ‘Button Text’ enter the text for the button e.g. Routine
− Press the ‘Select’ button to link a worklist file to the button.
The worklist file must be created before.
®
4 S TA R T U P & S E L F T E S T
Note: Use the Engineering mode for service.

• Power on the instrument and the computer.
• Logon to Windows XP with the correct user name and password.
(see also paragraph 4.1)
• Start the DAVINCI QUATTRO Software by a double mouse click on the
icon.
The PC and the instrument will “connect”. The database is prepared and a self-test
procedure that takes 60-80 seconds will commence. All modules are tested, the
horizontal and vertical barcode scanner are set up and the cover lock is tested. The
arm will move during this time, so the cover must be closed, or the safety key
inserted and safety precautions observed. When the tests are complete, the test
results will appear on the monitor.
The print command can be used to print a copy of the initial self test
results (3 pages).
An example of printout can be found at the end of this chapter.
The < File > - < Save As … > buttons can be used to save the
results to a file in the folder C: \ Program Files \ bioMerieux \
DAVINCI QUATTRO Program \ Selftests
The following actions are performed during the self test :

− Test of communication ports
− Test of Power Supply 12 - 24 - 48 Volt (GPS)
− Serial number of the modules is sent to the PC
− Firmware version of modules is sent to the PC
− Self test of each module (important for reader)
− Set motor speed settings (X, Y, Z, E, P motor)
− ESP settings are sent to PC (ESP database)
− Pipettor parameters are sent to PC (air gap, cal date, …)
®
Start Up & SelF Test
− Drawer coordinates and sensor offsets drawer are sent to PC

− Barcode type settings are sent to barcode scanners
(reading coordinates are saved in the software - not in instrument)
− Washer parameters are sent to PC
Reader test performed during the self test
• PLATE MOTOR TEST - This option is responsible for verifying the

functionality of the plate motor, front sensor and the rear home sensor.
This will be accomplished by checking the distance between the 2
sensors.
• FILTER MOTOR TEST OPTION - This option is responsible for verifying
the functionality of the filter motor and the filter wheel home sensor. This
will be accomplished by rotating the filter wheel at least one complete
rotation and checking the filter wheel index position.
• BACKGROUND LIGHT LEVEL TEST OPTION - This option is responsible
for verifying the light tight soundness of the reader and the accuracy of the
plate carrier dark read position. This will be accomplished by turning the
lamp off, verifying that all 12 photodiodes read between 520,000 < counts
< 525,000, turning the lamp on, moving the plate carrier into the dark read
position and verifying that all 12 photodiodes read between 520,000 <
counts < 525,000.
• LAMP VOLTAGE TEST OPTION - This option is responsible for verifying
the functionality of the lamp. It is accomplished by turning the lamp on and
reading the voltage drop across the lamp (the lamp A/D counts must be
less than 760).
• LAMP MARGIN TEST OPTION - This option is responsible for verifying
that the system will perform under degraded power conditions. This is
accomplished by moving to the filter 1 position and adjusting the lamp duty
cycle to maximum and ensuring the minimum channel reads
200,000 counts above the dark reading.
• LAMP CALIBRATION TEST OPTION - This option is responsible for
calibrating the lamp duty cycle at each installed filter wavelength. This is
accomplished by assigning all lamp duty cycles an arbitrary number
moving to each installed filter position and adjusting the lamp duty cycle
until the minimum channel reads 170,000 counts above the dark reading
and the maximum channel reads less than 275,000 counts above the dark
reading.
• EEPROM TEST OPTION - This option is responsible for verifying the
functionality of the EEPROM. This is accomplished by reading the entire
EEPROM and comparing the data to the EEPROM mirror values.
®
Incubator test performed during the self test
• PLATE MOTOR TEST OPTION - This option is responsible for verifying

the functionality of the plate motor, front sensor and the rear home sensor.
This will be accomplished by checking the distance between the 2 sensors
(380-400 steps).
• TEMPERATURE SENSOR TEST - This is accomplished by reading the
plate sensor and cover sensor and determining if the temperature is within
the valid temperature range.
• EEPROM TEST OPTION - This option is responsible for verifying the
functionality of the EEPROM. This is accomplished by reading the entire
EEPROM and comparing the data to the EEPROM mirror values.
Washer test performed during the self test
• HOME THE MOTORS (the manifold arm will move to the home position)
Drawer test performed during the self test
• HOME THE DRAWER MOTOR

• TEST THE DRAWER MOVEMENT TO DEFINE THE POSITION OF THE
SENSORS
The eject position is 9 mm from the out position
• EEPROM TEST - This option is responsible for verifying the functionality
of the EEPROM. This is accomplished by reading the entire EEPROM and
comparing the data to the EEPROM mirror values.
• TEST THE DRAWER TEMPERATURE SENSOR - This is accomplished
by reading the ambient sensor and determining if the temperature is within
the valid temperature range.
®
Pipette test performed during the self test
• HOME THE MOTORS - Move the Eject motor, Z motor, Pipette Motor to
the home position.
• TEST FOR TIP / PLATE PRESENCE
• TEST THE Z ENCODER
• TEST MAXIMUM P steps (at least 4300 steps)
• INITIALIZE THE PRESSURE ARRAY
XY module test
• HOME THE MOTORS (X and Y).
Sample ID module test performed during the self test
• TEST THE COMMUNICATION

• EEPROM TEST - This option is responsible for verifying the functionality
of the EEPROM. This is accomplished by reading the entire EEPROM and
comparing the data to the EEPROM mirror values.
At the end of the seft-test,

• Print the selt-test report using the "Print" command (see the example of
report on the following page.
®
Test1 Printed on 11/11/2007 at 1:13:43 PM Page 1 of 3

__________________________________________________________________________________________
Date: Tuesday, May 30, 2008

Time: 1:13 PM
Comms
Serial No. 1EPA1871

Version No. CM 1.02
ALL TESTS PASSED
AM Reader
Serial No. 1AXA1900

Version No. AM v1.21
ALL TESTS PASSED
AM Reader settings:
Filter 1: 405
Filter 2: 450
Filter 3: 620
Filter 4: 690
Filter 5: Not fitted
Filter 6: Not fitted
Drawer
Serial No. 1ADA1857

Version No. DM 2.20
ALL TESTS PASSED
Drawer settings:
Out to eject offset: 9.00 mm

Ramp profile: Initial: 246, Final: 3500, Acceleration: 25
Calibration Hole Location; X: 7731, Y: 1631, Z: 3423
Washer
Serial No. 1WMA1867

Version No. WM 1.15
ALL TESTS PASSED
Washer settings:
Number of wash head rows: 8
Number of wash head columns: 1
Number of dispense pumps: 4 (mistake only 1 dispense pump)
®

__________________________________________________________________________________________
Incubator 1
Serial No. 1IMA3395

Version No. IM v2.09
ALL TESTS PASSED
Incubator 2
Serial No. 1IMA3345

ALL TESTS PASSED
Incubator 3
Serial No. 1IMA3381

ALL TESTS PASSED
Incubator 4
Serial No. 1IMA3365

ALL TESTS PASSED
Pipette
Serial No. 1PMA2127

Version No. PM 1.05
ALL TESTS PASSED
Pipette settings:
Z Ramp home profile: Initial: 400, Final: 800, Acceleration: 15
Z Ramp slow profile: Initial: 400, Final: 3000, Acceleration: 30
Z Ramp fast profile: Initial: 400, Final: 7000, Acceleration: 65
E Home Ramp profile: Initial: 100, Final: 300, Acceleration: 6
E Ramp profile: Initial: 246, Final: 2000, Acceleration: 30
P Home Ramp profile: Initial: 200, Final: 2500, Acceleration: 30
P Micro liters per P Step: 0.319186
Fluid level detection gain pressure sensor setting: 300
Fluid level detection; Sample tip adjust offset: -6, Reagent tip adjust offset: -9, Undetectable region: 17
steps
Fluid level detection profile: Initial: 500, Final: 3000, Acceleration: 80, declaration: 250, Stop: 800
Maximum P steps: 4433
Low volume air gap steps: 30, High volume air gap steps: 63
Low volume air gap limit: 150 µl
®

__________________________________________________________________________________________
Home offsets, Z: 134, P: 30, E: 30

Backlash steps: 5, Blow out steps: 100
Maximum Aspirate volume: 1162.8 µl
Single shot sample tip volume calibration readings = 8
Single shot reagent tip volume calibration readings = 8
Multiple shot sample tip volume calibration readings = 5
Multiple shot reagent tip volume calibration readings = 8
Calibration date: Tuesday, September 11, 2008
XY Module
Serial No. 3904701475

Version No. XY 1.02
ALL TESTS PASSED
XY Module settings:
X Ramp home profile; Initial: 100, Final: 1000, Steps: 60
X Ramp slow profile; Initial: 100, Final: 5000, Steps: 450
X Ramp fast profile; Initial: 150, Final: 8500, Steps: 450
Y Ramp home profile; Initial: 100, Final: 1000, Steps: 60
Y Ramp slow profile; Initial: 100, Final: 3500, Steps: 350
Y Ramp fast profile; Initial: 100, Final: 6500, Steps: 350
Home Offsets; X: 206, Y: 70
Sample ID Module
Serial No. 1SMD1570

Version No. SM 2.20
ALL TESTS PASSED
®
5 MANUAL CONTROL
Access to the manual control

To access the manual control, use the following commands:
QUATTRO software Tools System Manual Control
A dialog box is displayed.
1. Click Yes to use the manual control.
The "Manual control" window is displayed.

2. Select the item to be controlled manually in the area on the left.
3. The possible actions are displayed in the area on the right.
4. Select the action and click on "Do it".
The figure above shows an example for the Reader.
®
Manual Control
Each function works as described above.
®
Manual Control
®
Manual Control
Dispense Test -Pipette Calibration settings (e.g. air gap)
From the Main Menu, select Tools - System Manual Control - Testing - Dispense
Test. The following screen appears.
This screen is only important to set the correct Pipette Calibration settings. It’s
important that the High volume air gap is set to 63 steps (= 20 µl).
To change the air gap volume :

− Enter “63” for “High vol. Air”.
− Press the button “Send values now”.
− Press “ Finish”.
If the aspirated volume in the tip is higher than or equal to 150 µl, the air gap is 20 µl
(63 steps) now. If the aspirated volume in the tip is lower than 150 µl, the air gap is
10 µl (30 steps).
®
Manual Control
Send Commands
From the Main Menu, select Tools - System Manual Control - Advanced - Send
command(s). The following screen appears.
The Command Direct screen allows you to send commands to the hardware
modules.
The commands can be used for troubleshooting and verification. An overview of all
the commands can be found in the appendix “Manual Control Codes”.
NOTE: Due to software updates the list is no longer complete; some

commands are not used and other commands are missing.
Module identifier :
D0 Drawer Module
S0 Barcode Scanner Module
I0 / I1 / I2 / I3 Incubator Modules 1-2-3-4
NOTE: If one of the modules does not answer, the 3 remaining modules will
be numbered as 0, 1 and 2.
W0 Washer Module
A0 Reader Module
X0 XY Controller Module
P0 Pipette Module
C0 Communication Module
CAUTION! The commands are a powerful tool. Some commands could

drive components in the module past their normal range. Other
commands may erase the memory of the device, resulting in a
loss of set points and calibration settings.
®
Manual Control
Some useful commands
Pipette
• To check the plate detect sensor in the pipette : P0RPS
Reply : P0RPS1 (plate present - flag in sensor) – P0RPS0 (no plate

present - flag out sensor)
To lock and unlock a plate carrier : P0PLK0 (unlock) – P0PLK1 (lock)

A plate carrier can be installed manually.
• To check the tip present sensor in the pipette :

P0RTSY (move to tip sensor to check tip presence)
P0RTSN (read sensor only)
Reply : P0RTS1 (tip detected - flag out sensor) - P0RTS0 (no tip detected
- flag in sensor)
A tip can be installed manually.
When using the command RTSY, the E-motor will move to the Tip sensor. If a tip is
present the flag will not reach the sensor, because the movement is blocked by the
plastic tip.
Incubator
If all the incubators are functioning, x=0 for incubator 1 - x=1 for incubator 2 - x=2 for
incubator 3 - x=3 for incubator 4:
• To switch on the incubator heaters:

IxHTR1370 (incubator bottom heater (1) to 37.0 deg. (370) (= Plate
Heater)
IxHTR2370 (incubator top heater (2) to 37.0 deg. (370) (= Cover Heater)
• To read the temperature : IxTMP1 (for bottom) – IxTMP2 (for top)
Reply : IxTMP137.0 (bottom at 37.0°C)
Reader
• To perform the selftest of the reader : A0STSa (to perform all 8 tests)
• To switch the lamp on / off : A0LMP1 (lamp on) - A0LMP0 (lamp off)
It’s difficult to see if the lamp is on or off (low intensity).
®
Manual Control
EPOD (Communication board)
• To check if all modules are present : C0MAP
Reply : MAP D0 S0 I1 I3 A0 W0 I2 I0 P0 X0
• To get the module calibration coordinates : C0SCDnn
nn Module nn Module
01 Reader 09 Incubator 3
02 Drawer, front left 10 Incubator 4
03 Drawer, front right 11 not used
04 Drawer, back left 12 not used
05 Drawer, back right 13 not used
06 Washer 14 not used
07 Incubator 1 15 not used
08 Incubator 2 16 not used
Reply : C0SCDnnxxxxxyyyyyzzzzzppis
Examples:
C0SCD01 01690 01330 00275 07 0 1AXA1900
C0SCD02 06540 03685 03565 03 0 1ADA1857
C0SCD03 09010 03680 03565 03 1 1ADA1857
C0SCD04 06540 01525 03565 03 2 1ADA1857
C0SCD05 09000 01525 03565 03 3 1ADA1857
C0SCD06 02120 00065 03255 08 0 1WMA1867
C0SCD07 06110 01345 02210 10 0 1IMA3395
C0SCD08 11460 01355 02210 05 0 1IMA3345
C0SCD09 06110 01325 00240 09 0 1IMA3381
C0SCD10 11450 01335 00250 06 0 1IMA3365
pp = port number
i = module number
s = serial number module
• To check the status of the power supply 12 V – 24 V – 48 V : C0GPS
Reply : C0GPS00 (all ok)
®
Manual Control
EPOD (XY board)
• To view the workspace calibration coordinates : X0CAL
Reply : X0CAL XXXXX YYYYY ZZZZZ xxxxx yyyyy zzzzz
Reply : CAL 12744 00238 03628 00050 03527 03623
XXXXX = back-right X-axis

YYYYY = back-right Y-axis
ZZZZZ = back-right Z-axis
xxxxx = front-left X-axis

yyyyy = front-left Y-axis
zzzzz = front-left Z-axis
• To view the home offsets (back left X-axis – back left Y-axis) : X0SHO
Reply : SHOxxxyyy
NOTE: Z home offset is stored in the pipettor.
Washer Module
• Check the dispense valve : W0VAL0500 (open for 500 ms) - reply OKY
• Check Waste sensor : W0LVA - reply OKY (empty) - ERRLVW (waste full)
• Check sensors in bottles : W0LVD - reply e.g. LVB (B empty) - OKY (level
in all bottles OK)
• Check status Vacuum switch : W0LVV - reply OKY (vacuum ok) -
ERRVAC (low vacuum)
• Get the home offset X,Y,Z of the washer : W0SHO - reply : W0SHO 015
030 037 026
Drawer
• To get the ambient temperature : D0TMP – reply D0TMP023.45 (for 23.45

°C)
• To get the drawer calibration coordinates :
− X coordinate : D0EEPR20 - reply EEPR20xxxxx

− Y coordinate : D0EEPR21 - reply EEPR21yyyyy
− Z coordinate : D0EEPR22 - reply EEPR22zzzzz
®
6 H A R D WA R E & TROUBLESHOOTING &
DISASSEMBLING
After installing a new module, the system will detect the new module during the self
test. The system is checking on the serial numbers of the modules. The software will
guide you automatically to the module calibration screen. The X, Y, Z coordinates of
the new module are set to 0, 0, 0.
Overview of actions to be done after module replacement :
MODULE CALIBRATION VERIFICATION

- Plate movement Test
Incubator - Module Plate Calibration
- Incubator verification
- Insert Plate Carrier and
Manifold
- Module Plate Calibration
Washer - Washer Verification
- Wash head offsets
- Washer Pump calibration A, B,
C, D
- Install filters
Reader - Enter Filter Settings
- Reader Verification
- Drawer Calibration - Plate movement Test
Drawer
- Pipette collar calibration
- E sensor calibration
- Workspace Calibration
- Tip Alignment Test
Pipette - Drawer Calibration
- Pipettor Verification
- All Module Plate Calibrations
- Read sample ID’s
- Pipette volume calibration
- ESP setup
- Read sample ID’s
Barcode Scanner - Sample Rack Scan Position
- Read plate ID
Communication PCB - All Module Plate Calibrations - Plate movement Test
- Workspace Calibration
Note: Due to a new workspace
calibration it's recommended - Tip Alignment Test
XY Drive PCB
to repeat the drawer - Plate movement Test
calibration and to check all
module plate calibrations.
®
Hardware & Troubleshooting & Disassembling
Incubator
6.1 INCUBATOR
Incubator Specifications
Number of 4
incubators
Temperature range RT + 7°C to 50° C

Temperature ± 1° C
Accuracy
Shaking >15 Hz periodic or continuous
Top (Cover) - Heater A

High speed warming : less than 1 min at 37 °C - overshoot 40.5 °C
Bottom (Plate) heater – Heater B

Low speed warming : ± 5 min to reach the set point 37.0 °C - no overshoot
• Both heaters (5.5 Ω) are heated at the same temperature.

• Temperature sensor in both heaters.
• 2 plate sensors :
− home sensor (flag - SP 45688549). The home position is also the
heat position.
− eject sensor (optical reflective - non SP) to drive the plate carrier to
the full extended position.
The distance in motor steps between the home and eject sensor must be between
380 and 400. This will be checked during the self test.
®
Incubator
• There is some degree of control over the shake speed used by the
incubators. A set of registry settings may be used to control the shaking. It
is imperative that the end user validates their assays with the final shake
settings selected.
Troubleshooting :
− damaged heater pads
− defective Incubator PCB
Under rare circumstances the connector on the heater flex cable from the plate
carrier may heat up and eventually singe the board causing temperature related
problems. The plate carrier movement causes the connector to move causing
resistance, which in turn will heat up the connector and eventually fail.
Resolution : Secure the cable to prevent movement of the connector. tions below.
Cut two Foam Tape. Peel the backing off of one of the foam tape strips and place it
along the edge of the slot in the chassis, centered under the heater flex cable. Place
the flex cable against the foam tape and apply the other piece of foam tape directly
on top of the existing piece, securing the flex cable between the foam strips. See
Figure.
®
Incubator
Field repair : The following parts can be replaced in the field :
• Incubator module
Remove the Incubator Module from the system by rotating the two Allen
screws counter clockwise ¼ turn and pulling the module from the system.
Install the new module and perform the plate alignment.
• Position Home sensor
The position home sensor (SP 45688549) is mounted on the base as
shown in the figure and is removed by unscrewing the two M3 x 6mm
Socket Cap screws and lock washers.
All other incubator problems (e.g. damaged heater pads, Incubator PCB, defective
eject sensor, plate carrier, belt, motor, ... ) can only be solved by replacing the
complete incubator module. The defective module must be returned to Dynex for
repair.
Special tools and Dynex Test Software are needed to repair the module.
Why no more field repair ?
− To install the adhesive heater foil an alignment jig is needed.

− After the replacement of the heater pads or the PCB, the temperature
needs to be calibrated with a calibrated thermometer and the settings
have to be stored into the PCB with the Dynex Test Software.
− Replacement of the eject sensor requires calibration using Dynex Test
Software.
®
Reader
6.2 READER
1. Lamp 6. Lenses
2. Heat Filter 7. Optic Stops
3. Lens 8. Microplate
4. Filter 9. Photodiode
5. Optic Fibers 10. Reference Diode
Reader Specifications
Photometric 0.000 to 3.000 OD

Range
Spectral 405 nm to 690 nm
Range
Precision ±0.010 OD at 0.000 to 0.500 OD
<1% CV at 0.501 to 2.000 OD
<1.5% at 2.001 to 2.500 OD
Linearity ±1% at 0.000 to 2.500 OD
≤1.5% at >2.500 OD
Accuracy ±0.01 OD or 2.5% (0.000 to 3.000 OD)
whichever is greater
#
Read Time <10 seconds, single wavelength
#
<20 seconds, dual wavelength
®
Reader
• Tungsten halogen lamp 6 Volts - 20 Watt (lifetime ± 3 years)

• Optical Fiber
• 12 channels + reference channel (J11 cable) to regulate the lamp intensity
• Filter wheel with 405 - 450 - 620 - 690 nm filters Position 1 is used to
perform the self test. The 405nm filter is the worst case and stresses the
system to the maximum. If the self test passes with the 405 then all should
be good as far as the lamp circuitry is concerned. For that reason the 405
nm filter MUST BE INSTALLED ON POSITION 1.
• 2 empty positions
• 4 optical sensors :
− Home Sensor (flag - SP 45688549). The home position is used to
perform the 100 % Transmission blanking.
− Eject Sensor (optical reflective) (non SP). To drive the plate carrier
to the full extended position.
− Plate In Sensor (flag - SP 45688549). To ensure that the plate
carrier has been retracted within the unit so that the DSX arm can
move without the possibility of collision.
− Filter wheel home sensor (switch – non SP)
The distance in motor steps between the home and eject sensor must be between
380 and 400. This will be checked during the selftest.
Other filters can be ordered :

410,415,420,490,510,520,530,540,550,560,570,585,590,595,600,610,630,650,655
nm
The reader measures the amount of light (T) that is transmitted through the sample.
Sample - 0% MIN (dark)

T = ------------------------------------------------ OD = Log 1 / T
100 % (blanking) - 0% MIN (dark)
ADC : 20 bit (1 048 576 counts)
®
Reader
Lamp PWM : The duty cycle is defined during the self test at 405 nm. This duty
cycle is used for all filters. The 100% light value and dark values are checked
against limits before each plate read to ensure each read is valid. If the 100% value
is out the lamp will be adjusted to bring it back within the limits if possible. If it is not
possible then you will get a lamp related error.
When a Plate Read command is received, the following actions occur :
− The lamp is turned ON and start a 3 second timer.

− The required filter is selected.
− The plate is retracted towards the HOME position.
− At the end of the 3 sec time period, take 100% transmission readings
on all channels (= blanking) (duty cycle 405 nm filter defined during
selftest). The 100% light value is checked against limits. If the 100%
value is out the lamp duty cycle will be adjusted to bring it back within
the limits if possible.
− The plate is moved to the DARK position (a number of motor steps of
the home sensor) and dark readings on all channels are taken. The
lamp is ON while the rear edge of the plate carrier is covering all the
light.
− The plate is moved to row A and wells 1 through 12 are ready.
− The plate is moved to each remaining row, all wells in each row are
read.
− When the plate read routine is completed, the plate is ejected and the
plate data is transmitted to the program.
®
Reader
Troubleshooting : Self test fails (bad lamp - focus problem (adjust lamp position))
Margin test fails

This is one of the errors we have frequently encountered with the daVINCI Quattro
instruments. During the selftest of the reader the lamp duty cycle is set to maximum
(= 140). The minimum channel must read 200,000 counts above the dark reading at
filter position 1 (405 nm).
The test might fail because :

− There is too much dust on the optics. Clean the lamp, the filters and the
lenses.
− Degraded lamp (low light intensity). Replace the lamp.
− Bad lamp position. Change the lamp position.
− Damaged 405 nm filter. Replace the 405 nm filter (LPD = 140).
The duty cycle is an indication for the status of the reader optics. Dust or any other
lightblockage will increase the duty cycle. By sending the command A0LPD, the
value of the duty cycle will be returned. Normal value 85 - maximum value 140.
When the LPD value is 140, a lamp margin failure will be displayed.
LAMP CALIBRATION - This option is responsible for calibrating the lamp duty cycle
for filter position 1 (405 nm). This is accomplished by assigning the lamp duty cycle
an arbitrary number, moving to filter position 1 and adjusting the lamp duty cycle
until the minimum channel reads 170,000 counts above the dark reading and the
maximum channel reads less than 275,000 counts above the dark reading.
®
Reader
Field repair : The following parts can be replaced :
− Reader Module
Remove the Reader Module from the system by rotating the two Allen screws
counter clockwise ¼ turn and pulling the module from the system.
The new module will come with the following filters: 405, 450, 490 and 620 nm.
Probably, some filters from the defective module (e.g. 690 nm) must be tranferred to
the new module. Reinstall the module. Enter the correct filter settings in the setup
screen and perform the pipettor plate alignment.
− Plate Home Sensor & Plate In Sensor

• The plate home sensor is removed by unscrewing the two M3 x 6mm
socket cap screws and two M3 lock washers.
• Removal of the Plate front sensor requires removing the lower slide
block and then removing the two M3 x 6mm socket cap screws and
two M3 lock washers.
• Place the sensors into the XY Base.

• Fasten the home sensor with 2 M3 x 6mm socket cap screws and 2
M3 lock washers.
• Fasten the Plate front sensor with 2 M3 x 6mm socket cap screws and
2 M3 lock washers and reinstall lower slide block.
®
Reader
− Fan
• Remove the fan from the optics cube by removing the 4 M3 x 25mm flat
head screws that secure it to the cube (see figure).
• Carefully cut away the RTV around the fan wires and remove the
connector from the wires.
• Pull the fan out of the cube.
• Mount the fan in the optics cube using the four M3 x 25mm flat head
screws and Loctite 242.
• Install the fan to blow into the cube with the wire going out the access hole
on the right. Attach the 2 mm 2-pin connector to fan wires using 2 contact
crimps.
Pin 1 connects to the Black wire and Pin 2 connects to the red wire. The
connector has a small notch that indicates the position of Pin 1. The wires
should be twisted 1 turn per inch before termination. The final length of the
terminated wires should be 5.25 inches.
− Lamp
• Remove the Reader Module from the system by rotating the two Allen
• Remove the Filter Access panel on the rear of the Reader Module to
expose the optical assembly. The bulb is mounted in the upper portion of
the optical assembly.
CAUTION! WEAR POWDER FREE GLOVES WHEN THE LAMP IS BEING

REPLACED. GLOVES ARE NECESSARY TO PREVENT SKIN
OILS FROM DAMAGING THE LAMP.
• Grasp the lamp with plastic tweezers and pull the lamp out of its
receptacle.
®
Reader
• Grasp the lamp tines of the new lamp with a pair of needle nose pliers and
align the tines with the corresponding holes in the lamp socket as shown
in the figure and firmly insert the lamp into the socket.
NOTE: If you see any fingerprints or foreign material on the lamp, remove
them using a lint-free cloth saturated with methanol.
• Reinstall the Filter Access Panel and reinstall the Reader Module in the
system.
• Run the selftest. When it fails, adjust the lamp position. To adjust the lamp
position a power extension cable might be useful to avoid the reader has
to be installed several times. (the low level command STSa can be used).
− Filters
• Remove the Reader Module from the system by rotating the two Allen
• Remove the Filter Access Panel on the rear of the Reader Module to
expose the optical assembly. The filters are mounted on the filter wheel as
shown in the figure.
• Locate the filter that is to be removed. Firmly grasp the exterior filter
housing with a pair of needle nose pliers and pull the filter out of the
spring-loaded slot.
Note: The filter 405 nm must be installed on position 1.
®
Reader
• To install a new filter, firmly grasp the exterior filter housing with a pair of
needle nose pliers and push the filter wheel into the spring-loaded slot.
• Reinstall the filter access panel and reinstall the Reader Module in the
system.
• When adding a filter, the new wavelength has also to be entered in the
software. Go to DAVINCI QUATTRO Software - Tools - Configure system
- Absorbance Reader. Enter the new wavelength.
• Run the selftest.
NOTE: The bottom groove of the filter must be firmly seated in the filter
wheel.
If the groove is aligned with the spring on the filter wheel, the filter has
been installed incorrectly and will result in invalid instrument
performance.
All other reader problems can only be solved by replacing the complete reader
module. The defective module must be returned for repair. Special tools and
software
(Dynex test software) are needed to repair this module.
• After the replacement of the PCB, the module needs to be calibrated and
tested using the Dynex Test Software.
• Replacement of the eject sensor requires calibration using Dynex Test
Software.
• Replacement of the optics, fiber, plate carrier, motor, belt needs special
calibration tools and Dynex Test Software.
®
Washer
6.3 WASHER
Washer Specifications
Manifold 8-way wash head

configuration
Programmable 50 – 999 µl
volumes
Wash 4 wash bottles at 2.0
containers L, with level-sensing
Waste 8 L with waste full
Container sensor
Residual wash <3 µl per well with a
volume sweep in a flat-bottom
plate
Dispense ≤5% CV (with 300 µl in
precision a 96 well plate)
• 8 aspirate and dispense needles

• Different types of microplates can be used (different wash settings stored
in plate settings)
• Wash head can be moved in X, Z and plate carrier can be moved in Y
direction
• 2 microswitches to detect the X-Z home position
• 2 Optical sensor (flag - non SP) to detect the Y home position and plate
out position.
• Dispense volume (50-999 µl) : timing pinch valve
• Sweep option (normal / super sweep) :
− No sweep : aspiration only in center of well)

− Normal sweep : aspiration left, right, middle of well
− Super sweep : aspiration on 6 positions in the well (not used)
Sweep stroke (e.g. 3 mm)
Sweep height / sweep stroke plate setting (plate database)

Selection of the use of a sweep assay setting
• Main Washer PCB Board & Daughter PCB (controls the XYZ drives,
sensors, pinch valve)
®
Washer
®
Washer
Troubleshooting :
− X / Z - microswitch can break (! Transport)

− Pinch valves
− Noisy vacuum pump : maintenance kit for pump available with seals,
valves, …
− The dispense pump is not priming empty lines.
See the adjustment procedure 7.2.2.
− Washer module
• Remove the manifold, tubing and plate carrier.
• Remove the Washer Module from the system by rotating the two Allen
• Reinstall the module, plate carrier, tubing and manifold.
• Perform the pipettor plate alignment, the pump calibration for the 4 bottles,
the calibration of the wash head offsets.
− Pinch valve, washer

• Remove the Valve Mounting Assembly, which is mounted to the front
cover of the Washer Module (see figure).
• To remove the assembly, remove the four M3 Nyloc Nuts and M3
washers.
®
Washer
• Remove the Ring Terminal by removing the M4 Nyloc Nut, M4 flat washer
and M4 External tooth washer (see figure).
• Withdraw the pinch valve thru the access hole in the front cover (see
figure).
• Disconnect the Pinch Valve Harness.

• Install the new pinch valve in reverse order.
®
Washer
− Wash X Microswitch
• The X-Drive Microswitch is mounted on the left plate. To remove the
microswitch, remove the two M2 x 12mm Pozi Panhead screws, which
mount the microswitch to the plate..
• Unsolder the two wires from the existing

switch and re-solder them to the replacement
switch.
• Secure the switch using the two screws
removed in Step 1.
Note: When you reconnect the wires, use
good soldering technique and make
certain that the junction is protected
via shrink-wrap.
− Wash Z Microswitch
• The Z Microswitch is a mounted on the Z-drive assembly in the upper left
corner.
• To replace the Switch, disconnect the two M2 x 12 mm Pozi Pan screws.
• Remove the solder the wires from the switch and re-solder to the new
switch.
• Secure the switch using the two screws removed in Step 1. Push the
switch upwards when tightening the screws.
®
Washer
− Wash head
• The wash head can be removed from the washer by lifting the manifold
and disconnecting the tubes.
• When a new wash head is installed, adjust the horizontal position of the
needles by the screw on top of the manifold.
− Vacuum switch
• The vacuum switch is connected to the left side of the unit as shown in the
figure. To have access to the vacuum switch, remove the reader module.
• To remove the vacuum switch disconnect the silicon tubing from port A on
the vacuum switch, disconnect the wires, and remove the switch.
• Attach the silicon tubing to port “A” on the new vacuum switch.
• Recalibrate the new vacuum switch. During calibration, the waste bottle
must be empty and the washer module with the wash head must be
present.
®
Washer
− Vacuum filter, in-line

• The vacuum filter is connected to the left side of the unit as shown in the
figure. To have access to the vacuum filter, remove the reader module.
• Disconnect the vacuum filter from the tubing.
• Attach the Vacuum Filter between the other end of the tubing and the free
end of the tubing from the front extrusion. Ensure that the direction of the
arrow on the vacuum filter is away from the vacuum switch as shown in
the picture.
− Vacuum Trap
• The vacuum trap is connected to the left side of the unit as shown in the
figure.
• Open the left side panel by removing the 4 screws.
• Disconnect the vacuum trap from the tubing.
• Reinstall a new vacuum trap
®
Washer
− Dispense pump
• The dispense pump is connected to the left side of the unit as shown in
the figure.
• Open the left side panel by removing the 4 screws.
• Disconnect the dispense pump from the tubing.
• Disconnect the power connector.
• Reinstall the new pump and calibrate the pump for the 4 bottles.
− Aspirate pump
• The Aspirate Pump Assembly is located at the bottom of the system as
shown in the figure. To remove the pump assembly, unscrew the four M4
x 8 SKT cap screws.
• Disconnect the tubing and remove the pump from the housing.
• When replacing the pump, secure the screws with Loctite 222. Ensure that
the wires and the tubing from the straight barb fitting on the pump are
passed thru the grommet.
®
Washer
− Silencer, Noise reducer

• The Silencer is located at the bottom of the system near the aspirate
pump.
To remove the silencer, unscrew the four M4 x 8 SKT cap screws.
• Disconnect the tubing and remove the silencer from the housing.
• When replacing the silencer, secure the screws with Loctite 222.
− Vacuum relief valve
• Open the front extension (needs some power - be careful for your fingers).
Front
extension
• Disconnect the red vacuum relief valve from the tubing.

• Reinstall a new valve.
®
Washer
− Pinch solenoid valve, wash bottle
• Open the front extension (needs some power - be careful for your fingers).
Front
extension
• Remove the coil by removing the plug connector and the washer.
• Disconnect the cables.
• To remove the pinching device, unscrew the two screws.
• After installing a new pinch valve, recalibrate the bottle for this pinch valve.
®
Washer
− Other parts that can be replaced very easily
Air Filter
Spring, plate carrier washer
Container, waste liquid
Container, washbuffer
Tubing
Coupling, elbow, wash bottle
Coupling, female large, waste
Coupling, female small, waste
Coupling, large elbow, waste
Coupling, small elbow, waste
Fitting, left, waste container
Fitting, right, waste container
O-Ring, fitting, waste container
Connector, 4 pole female socket
®
Washer
After the replacement of the main PCB and Y sensors, the module needs to be
calibrated and tested using the Dynex Test Software.
The pumps
− KNF Dispense Pump : type PML5094 - NF30 (micro membrane pump -
12V DC)
− Rietschle Thomas Vacuum Pump : type 107CDC20 D

(Diaphragm pump with 12 V DC motor)
• Red wire: always 12 V

• Black wire: floating – the line is connected to ground to start the pump
®
Drawer
6.4 DRAWER
3 optical sensors (flag - SP 45688549) : Home - Mid - Out
• Home = plate is fully inside.

• MID = The plate is half out. Access to front plates. Plates are still locked.
• OUT = The plate is fully out. Access to all plates. But, plates are locked.
Eject position = 9 mm from out position. Plates are unlocked.
Temperature sensing :
Sensor at the rear of the module. But, sensor is calibrated at the middle of the
drawer using the Dynex Test software. That means temp sensor and the PCB can
not be replaced in the field.
Temp. Sensor PCB
Calibration point
Troubleshooting :
− only 1 guiding rail
NOTE: For both modules, drawer and barcode scanner, the PCB and the
firmware are identical. There is a command to set the operating mode
of the firmware in drawer (DM) or scanner (SM) mode. See page 6-11
“Appendix Manual Control Codes”.
The command "SOMS" will set your module to Scanner mode. The
module name will become S0 or S1 depending on the number of
modules present in the system. The command "SOMD" will set your
module to Drawer mode. The module name will become D0 or D1
depending on the number of modules present in the system.
e.g. If your scanner is set into drawer mode, the C0MAP command
will show you the following modules : D0 D1 I1 I3 A0 W0 I2 I0 P0 X0
To fix this problem, enter the command D1SOMS.
®
Drawer
− Drawer assembly
Remove the Drawer Module from the system by rotating the two Allen screws
counter clockwise ¼ turn and pulling the module from the system.
After installing a new module calibrate the drawer and the plate coordinates.
− Sensors
• Unfasten the sensor by removing the two socket head screws and lock
washers.
• Disconnect the sensor cable from the ambient drawer main circuit board.
• Remove the cable through the access hole, noting the position of the
access hole. Note where the cable is secured with a tie wrap.
• Secure the new sensor into position, using the two socket head screws
and lock washers.
• Route the connector through the access hole noted in Step 2.
• Replace any tie wraps.
• Plug the connector into the PCB connector.
All other drawer problems can only be solved by replacing the complete drawer
module. The defective module must be returned for repair.
Special tools and software (Dynex test software) are needed to repair this module.
®
Barcode Scanner Module (SID module)
6.5 BARCODE SCANNER MODULE (SID MODULE)
− Vertical Laser Class II barcode scanner for scanning the sample tubes
− Horizontal LED CCD barcode scanner for scanning the microplates
Configuration
Default setup available:
EAN-8
EAN-13
IATA (not for vertical scanner)
MSI / Plessey
UPC-A
UPC-E
WPC (not for vertical scanner)
2 of 5
Codabar
Code 39
Code 93
Code 128
• Sample Rack Scan Position must be entered during the installation. The
manufacturer has defined the X, Y, Z scanning positions.
A document with the correct coordinates is included with the instrument /
module.
• For the Plate Scan Position the default settings can be used.
− Barcode scanner assembly
• Remove the Barcode scanner assembly from the system by rotating the
two Allen screws counter clockwise ¼ turn and pulling the module from the
system.
• After installing a new module enter the new coordinates for the sample
rack position in the DAVINCI QUATTRO Software (form with coordinates
included with the scanner).
®
EPOD
6.6 EPOD
This module supplies the power to the system (Power supply), performs the
communication between PC and the various modules (Communication PCB) and
contains the XY Drive PCB.
The workspace calibration is stored on the XY Drive PCB and the module plate
coordinates are stored on the Communication PCB.
Power Supply
V1 = 12.2 ± 0.1 VDC

V2 = 24.6 ± 0.1 VDC
V3 = 23.8 ± 0.1 VDC
V3 V2 V1
Brown Orange Red
The calibration of the power supply has been done by the manufacturer. No
adjustment is necessary at installation.
Each voltage V1, V2 and V3 can be adjusted by a potentiometer. The adjustment is
done without load. The voltage is measured at the power supply.
Communication PCB RS232 connected to J3 of Communication PCB

RS232 connected to J3 of Communication PCB
XY Drive PCB
®
EPOD
Field repair : The following parts can be replaced : Fan, Power Supply, XY Drive
PCB, Communication PCB and Communication PCB Firmware.
If needed, other parts like capacitor, inlet switch, inlet filter can be ordered after
contacting GCS.
To replace components, the EPOD Assembly has to be removed first.
• Remove the line power cord and the communications cable to the
personal computer from the EPOD.
• Remove the drawer module.
• Remove the racks from the right side of the workspace and then remove
the plate on the right rear side of the workspace.
• Push the EPOD from the rear to release it from the back plane board.
Considerable force is required to release the connectors from the socket in
the back plane board.
• Once the EPOD is freed from the back plane board, continue pushing it
toward the front of the unit and angle it up to remove it from the system. It
may be necessary to open the front extension to allow enough room for
the EPOD to be removed.
− Fan
The fan and fan grill are fitted on the EPOD housing.
• The fan is secured using four M3 X 35mm Pan Head screws with four M3
Flat washers and four M3 Nyloc Nuts. The red wire from the fan is
attached to V3 positive (+) and the black wire from the fan is attached to
V3 negative (-) on the power supply.
• When reinstalling the fan, feed the wires of the fan through 175mm of
Shrink Wrap Jacket.
®
EPOD
− Power supply
• Open the EPOD assy
• Remove the four M4 X 6mm screws and four flat head washers that attach
the power supply to the enclosure.
• Remove the Power Supply Unit (PSU) filter harness to the AC input
terminals
(see figure) of the power supply as follows :
− Blue wire ring terminal to the Neutral terminal on the PSU
− Brown wire ring terminal to the Live terminal on the PSU
− Green/yellow ring terminal to Earth terminal on the PSU
®
EPOD
• Remove the backplane harness and PSU harness from the DC output
terminals of the Power Supply Unit (see figure).
• To reinstall a new power supply, connect the Back Plane harness, PSU
harness and DC output terminals as follows :
1. Attach two double red wires of harness to V1 Positive (1 ring terminal to each
screw).
2. Attach black wires of harness to V1 Negative (double wires on the left and
single wire on the right).
3. Attach double orange wires of harness with the short blue harness, and
yellow wire end of harness to V2 Positive.
4. Attach double grey wires of harness and small lug end of blue wire to V2
Negative.
5. Attach double brown wires of harness and red wire from fan to V3 Positive.
6. Attach other end of the short blue harness and black wire from fan to V3
Negative.
7. The single yellow wire from the backplane harness and the free end of the
yellow arness with the diode should be attached to the positive (+) terminal
of the capacitor. When the backplane harness is installed, make certain that
it is secured in a manner so that is not in contact with Crystal (X1) on the
Communications PCB.
8. The free end of the single blue wire harness with the large lug should be
attached to the negative (-) terminal of the capacitor. Use two terminal
screws and two lock washers supplied with the capacitor (the lugs are placed
on top of the lock washers, tighten to 12 in/lbs with a torque driver.
• Place the PSU in the enclosure and use the four M4 flat washers and four
M4 x 6 button head screws to attach the PSU to the body. Use Loctite 222
to secure the screws.
NOTE: Before closing the Power Supply Unit, make certain that the ground
wires from the filter, the power input and the power supply filter
harness are firmly connected to the ground.
®
EPOD
• XY Drive PCB
The XY Drive PCB is used in the movement of the X motor and the Y motor
(either motor can be driven individually or both can be driven together). The board
is mounted on top of the communications board as shown in the figure.
• To remove the XY Drive PCB,

remove the four M3 X 6mm cap
head screws and four M3 washers.
• When replacing the board, partially
tighten the screws and then align
the board.
• Tighten the four screws.
• Readjust the workspace calibration.
• Communication Board
The Communication PCB receives information from the host computer and transmits
it to the various devices on the DAVINCI QUATTRO (e.g. the incubator).
In addition, the communication board receives information from each of the various
devices and transmits it to the host computer.
• Remove the XY Drive PCB as described above.

• Remove the four standoffs from the communications board as shown in
the figure.
• Remove the four M3 Nyloc Nuts and the four M3 plastic washers.
• Disconnect the three communication cables that connect the
communications board to the right side panel.
• When replacing the board, partially

tighten the screws and then align
the board.
• Tighten the screws
• Readjust the module coordinates.
®
Pipette Module
6.7 PIPETTE MODULE
Pressure
Sensor
− The tubing to the syringe is always dry.

− 3 sensors to check the Eject - motor position :
− Home
− To pickup the plate carrier
− To pickup a plastic tip
− 1 sensor for the home position of the P-motor
Troubleshooting
− Sticky collar problem
• Clean bearing with alcohol solution. The collar should fall down by
gravity
• If cleaning doesn’t help , replace the module
− Bad pipettor PCB, E-motor (eject) or P-motor (pipettor) problem

• replace pipette module
LED flashing = pipette module OK
®
Pipette Module
Field repair
− Pipette module
• Remove the Pipette Cover by removing the five M3 x 6 BHCS screws

and five
M3 shakeproof washers (see figure). Be aware that a new spare
module is coming without pipette cover.
• Remove the cable from the pipette controlelr Board (see figure). You
need a lot of force to remove the connectors. Be careful not to damage
the connector and the cable.
®
Pipette Module
• Remove the Pipette Assembly from the Z-Drive Assembly by removing

the two M3 x 12 SHCS screws that attach the Pipette Assembly to the
Drive Assembly.
The block is pinned, so rocking the pipette from left to right will frequently aid in
removing the module from the Z mount.
®
Pipette Module
Alignment of the pipette
The pipette module must be aligned on site when replacing the pipette
module, by loosening the 2 adjustment screws, accessed through the
controller board.
To align the pipette to the base plate, the Pipette alignment fixture (45688566) is
necessary. The Pipette base plate should be fitted on the jig as shown in the figure.
The Pipette alignment fixture should be positioned on the workspace in the reagent
rack location, using the fool-proofing.
®
Pipette Module
To align the pipette

• If Front/back alignment is needed (unusual), loosen the four screws on
the right hand side for adjustment. NB : to be able to do so, the covers
must be removed.
• Loosen the two screws, accessed through the controller board , for
Right/left adjustment.
• Gently move the X arm, Y arm, and the pipette until the pipette is
inserted flat inside the jig. The pipette base plate should fit perfectly in
the jig before proceeding to the next step.
• Tighten the two clamps to secure the pipette firmly to the jig.
• Remove each of the two adjustment screws (one at a time), then re-
install and tighten each screw using Loctite 222.
• Release the clamps, and ensure that the pipette bottom plate is
parallel to the fixture. If the pipette bottom plate is not parallel to the
fixture, readjust as described above.
• Remove the fixture from the system.
After installation of a new pipette assembly: align the pipette (see above), perform the
pipette collar calibration, the E-sensor calibration, the workspace calibration, the drawer
calibration, the plate alignments, enter the volume calibration factors and the ESP
curves (if used). Repeat the pipettor verification.
®
Removal of the system covers
6.8 REMOVAL OF THE SYSTEM COVERS
6.8.1 Top Cover Assembly
• Remove the seven M5 x 10mm BHCS screws that attach it to the

system.
• Remove the cover.
6.8.2 Rear Cover Assembly
• To be able to remove the rear cover, the top cover must be removed
first
(see procedure above).
• Remove the eight M5 X 10mm BHCS screws that attach the back
cover to the system.
• Disconnect the fan cables from J25 and J26 on the back plane of the
system while removing the cover.
®
6.8.3 Blue Cover Assembly
• Place the cover assembly in the open position (the gas springs are fully
extended in this position).
• Remove the three M8 X 25mm screws that connect the cover to the hinge
assembly on each side of the cover.
• Spread the cover assembly slightly to clear the hinge assemblies, then lift
the cover off of the unit (2 persons are needed).
To Reinstall the Cover Assembly :
• Hold the cover in the open position while spreading the cover assembly
slightly to clear the hinge assemblies.
• On one side of the cover assembly, align the Outer Cover Disc with the
holes in the cover. Loosely install three M8 X 25mm BHCS screws and
three M8 flat washers. Repeat for the other side.
• Gently push the cover up as high as it will go. Hold in this position while
tightening the six screws.
• If there is a gap between the cover assembly and the front extrusion when
the cover is closed, repeat Step 3, then check again. Ensure that the cover
lock operates properly after the installation.
®
6.8.4 Right side panel
• Remove the top cover and the rear cover.

• Remove the blue cover assembly.
• Remove the right cable cover by removing the four M4x8 BHCS screws
(see figure).
• Remove the three M6 X 12mm BHCS screws that attach the right side
panel from the top of the base plate (two in front, one in rear).
• Remove the M6X25mm screw and M6 flat washer from the upright
extrusion spring nut.
®
XYZ drive
6.9 XYZ DRIVE
Flexi cable X-Drive 34 way Ribon flat cable

45688523
XY Drive PCB
Y-Transition PCB X-Transition PCB
(in EPOD)
45688541 45688539
45688540
Flexi cable Y-Drive

45688524
Z-Transition PCB
45688542
Flexi cable Z-Drive

45688525
Pipette Assy
4568
The Belt Tension
The tension of a belt can be adjusted by using a tension meter (example: Brecoflex
Model SM4). However, some experience to measure the belt tension is required.
Get the average by measuring the tension 10 times.
Specifications :
X = 25 ± 1 Hz
The tension is measured in the middle of the non-carriage side of the belt.
The position of the arm can be either end (completely left or right).
Calibration procedure page 294.
Y = 60 ± 5 Hz
The tension is measured with the carriage furthest away from the Y-motor
(= front position). The cable bracket must be removed for measurement.
Z = 95 ± 5 Hz
The tension is measure with the carriage (pipette) held in the full-up position.
®
XYZ drive
Troubleshooting
The transport blocks after an init. Normally, the pipettor has to move
to the right front corner. Replace the Y- transition board.
X-Belt tension
Spring : lubricate
Grounding : Grounding of the power switch / base of pipette < 2.5 Ω
Field repair
− Z-Drive assembly
• The Z-Drive Assembly is attached to the Y-Drive Assembly with four

M4 x 10 cap head screws and M4 flat washers.
• When the Z-Drive Assembly is reattached to the Y-Drive Assembly,

make certain that the Z-Drive Assembly is held tightly against the
datum edge. After the screws are tightened, secure them with Loctite
222.
• After installation of a new Z-drive assembly perform the workspace
calibration, the Drawer calibration and plate alignments.
®
XYZ drive
− Z-drive spring
• Remove the lower cover for the Z-Drive (refer to the Replacing of the
Pipette).
• Remove the 4 M 3 x 30 flat head screws that attach the cover to the
mechanism.
• Remove the two M3 x 12 Posi screws.
NOTE: When reassembling the Spring Assembly housing, rotate the cover
eight times counter-clockwise before attaching the screws.
• Remove the spring Retainer Assembly from the Drive.
CAUTION! When removing the spring from the housing, be aware that the
spring will expand rapidly and may cause injury.
NOTE: When reinstalling the spring assembly, make sure that the eye lines
up with the groove in the shaft and take care to ensure that the spring
remains inside the housing.
The Z-Drive Assembly
• Carefully remove the Rotary Spring and place a new one on the
housing as shown in the figure, allowing the retainer to slide off while
pushing the spring into the housing fully. Discard the retainer.
®
XYZ drive
• Move the Z drive block to the end of the rail closest to the motor.
Ensure it remains there while winding the spring.
• Place the spring assembly onto the Z assembly, ensuring that the eye
lines up with the slot in the Z idler shaft and being careful not to push
the spring out of the housing. Orient the housing so that the two screw
holes on the side of the housing are facing towards the end of the Z
assembly extrusion.
• Attach the cover to the housing using two M3x12 flat head screws.
• Rotate the spring assembly eight complete revolutions counter-
clockwise
(as observed from the spring assembly). Secure the assembly to the
extrusion using four M3x30 flat head screws.
NOTE: Ensure that the assembly is square and flush with the extrusion and
that the two tapped holes in the housing are on the bottom when
completed.
− Bearing ball, Y drive

The Idler Pulley is made from two flanged bearings as shown in the figure.
To remove the bearing, unscrew the M3 x 6 cap head screw and flat washer.
When replacing the bearings, use Loctite 222 and secure the screw.
The Y Idler pulley is made of 2 bearings.
®
XYZ drive
− Replacement of the encoders
Hex key 1 mm – encoder in open position – turn motor position until the screw can
be released.
− 1 point : open (removal position)

− 2 points : closed (operating position)
®
XYZ drive
− Motors
X-Motor
• To have access to the X-Axis motor, remove the top cover, the rear
cover, the blue system cover and the right side cover of the system
(see procedure 6.8).
• Remove the cover of the EMC box (X transition board) - 4 screws.
• Disconnect the X-motor connector J1 from the X-transition board.
• Remove the three M4 x 16 SKT cap screws that attach the motor to
the motor mount. The motor axis is positioned ± 7 mm inside the
bearing (under the pulley). The motor can be lifted by moving the
motor from left to right and puling.
After a while the motor axis is coming outside the bearing.
• Remove the X-belt from the pulley and remove the motor from the
system.
• Remove the X-motor Pulley from the old motor by removing the 2
setscrews. You need some force to remove the pulley from the axis.
Be careful not to damage the pulley.
• Fit the pulley to the new X-motor using new M4X6 Setscrews and
Loctite 222. Discard the old setscrews. The distance between the
pulley and the motor base must be 3.8 mm.
• Reinstall the X-belt and the motor. Secure the screws using Loctite
222. Ensure that the motor wires are facing to the rear of the system.
• Connect the X-motor connector J1 to the X-transition board and close
the EMC box.
• Reinstall the covers.
®
XYZ drive
Y-Motor
• To be able to have access to the Y-motor, remove the top cover and
the rear cover of the system (see procedure 7.8).
• The Y-Axis motor is attached to the Y-Axis extrusion via two M3 x 8
mm cap head screws and two M3 x 10 mm cap head screws as shown
in the figure.
Remove the 4 screws.
• Disconnect the connector from the encoder.
• Remove the cover of the EMC box (Y transition board) - 3 screws.
• Disconnect the Y-motor connector from the Y-transition board.
• Remove the Y-belt from the pulley and remove the motor from the
system.
• Remove the encoder from the old motor and place it on the new motor
(See procedure). Ensure that the connector is facing to the right of the
system.
• Remove the Y-motor Pulley from the old motor.
• Fit the pulley to the new Y-motor using 2 new M4X6 Setscrew and
• Reinstall the Y-belt and the motor. Secure the screws using Loctite
222.
Ensure that the motor wires are facing to the left of the system.
• Connect the Y-motor connector to the Y-transition board and close the
EMC box.
• Connect the encoder.
• Reinstall the cover
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XYZ drive
Z-Motor
• To have access to the motor, remove the 3 z-drive covers (top, mid
and bottom).
• Disconnect the flexi cable from the top of the Z-transition board. Be
careful not to damage the connector.
• To remove the Z-motor assembly, unscrew the four M3 x 30 Flat head
screws.
• Disconnect the Z-motor connector from the Z transition board.
• Remove the Z-belt from the pulley and remove the motor from the
system.
• Remove the Z-motor Pulley from the old motor by removing the 2
setscrews.
• Fit the pulley to the new Z-motor using new M4X6 Setscrews and
• Reinstall the Z-belt.
• When reattaching the motor, use Loctite 222 on the screws and ensure
that the wires are facing to the bottom of the system.
• Connect the motor connector to the Z-transition board.
• Connect the flexi cable to the top of the Z-transition board.
• Reinstall the covers.
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XYZ drive
NOTE: The Drive Motor Pulleys and Idler Pulley must be properly positioned
on their respective shafts. Otherwise, the X-, Y-, or Z- Drive Belt may
contact the edge of one or both pulleys during operation.
• Observe the Drive Motor Pulley while manually moving the drive back and
forth along the drive axis.
• If the drive belt contacts the edge of the Drive Motor Pulley while the drive
is moved back and forth, stop at the point where the drive belt begins to
contact the edge of the pulley. Then, loosen the two M4 x 6mm cone
screws that secure the Drive Motor Pulley to the shaft, slide the pulley 2
mm away from the edge of the belt, and retighten the two screws.
• Perform an Arm self test by selecting “DSX Manual Control” from the
Tools menu, responding “Yes” to the prompt, and then selecting “Arm” in
the left panel and “Self-Test the XY” or “Self-Test the Z” (as appropriate) in
the right panel. Then, select “Do It” to begin the test. During the test,
observe the drive belt to determine if it rides near the center of the pulley
through its travel.
• Repeat steps 2 and 3 until the belt rides near the center of the pulley. If
the results are satisfactory, replace the M4 x 6mm cone screws with new
screws one at a time, replacing and tightening the first screw before the
second is replaced. Use Loctite 222 on the new M4 x 6mm cone screws.
• Repeat Steps 1 through 4 for the Idler Pulley.
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XYZ drive
− PCBs
X-transition PCB
• Remove the top cover, the rear cover, the blue system cover and the
right side cover (see procedure 6.8).
• The X-transition board is mounted in the EMC box on the left side of
the system.
• Remove the EMC box cover (4 screws).
• Remove the two M3X6 Cap Screws and washers that secure the heat
sink on the underside of the X-transition PCB to the right frame panel.
• Disconnect the solenoid cable from J9 on the X-transition PCB.
• Remove the four M3 Nylock nuts that secure the X transition PCB to
the standoffs.
• Reinstall the new board.
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XYZ drive
Y-transition PCB
• Remove the system top cover (see procedure 6.8).
• The Y-transition board is mounted in the EMC box.
• Remove the EMC box cover (3 screws).
• Disconnect the motor cable from J1, the encoder harness from J6 and
Y-Axis Optical Interrupter from J5.
• Remove the 2 M4 Nylock Nuts and M4 Nylon Washers that attach the
board to the EMC box.
• Remove the IC Mounting Plates to the Y extrusion using 2 M3 x 8
button head screws.
• When you replace the board, slide insulation between the motor driver
IC plates and the Y extrusion and secure the IC mounting plates to the
extrusion and assemble in reverse order.
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XYZ drive
Z-transition PCB
• Remove the Z-axis top, lower and mid cover.
• The Z-transition board is attached to the Z Flexi Cable Cover.
• Remove the two M3 x 8 button head screws and Nylon shoulder
washers.
• Remove the two M3 x 6 button head screws that hold the board to the
standoffs.
• Disconnect all connectors.
NOTE: When replacing the board, make certain that the insulation pads are
placed between the Drive IC blocks and the flexi cable cover.
®
XYZ drive
− X-belt
• Loosen (by one-half turn only) the four screws that secure the
adjustable block to the X carriage.
• Remove the adjustment screw completely.

• Loosen the adjustable block and the fixed block enough to free the
ends of the belt.
To Replace the X-Drive Belt :

• Fit the Fixed X Belt Block with the belt to the end of the datum face.
Ensure that the belt is properly located and flush with the end of the
block. Use 4 M3 x 12 skt cap screws; apply Loctite 222 and secure the
screws.
NOTE: The threaded hole must face to the left.
®
XYZ drive
• Route the belt around the pulleys, then fit the Adjustable X Belt Block
to the left end of the datum block. Make sure that the belt is properly
located and flush with the end of the block and affix using 4 M3 x 12
skt cap screws, 4 M3 Washers and Loctite 222.
• Fit the adjustment screw through the adjustable block and into the
fixed block.
• Tighten the X-Belt according to the following procedure.
To tighten the belt and check the belt tension :

• Position a Tension Meter (example: Brecoflex Model SM4) over the
rear section of the X belt as shown in the figure.
NOTE: The sensor must be 3 to 6 mm above the belt and perpendicular to
the belt.
• Tap the belt to cause it to oscillate.

NOTE: Note : The belt must only oscillate in the horizontal plane. If the belt
twists, the readings will not be correct.
• While the belt is oscillating and the sensor is above the belt, press and
hold the red button on the tension meter.
• Record the resulting tension reading.
• Repeat the above steps until 10 valid readings are recorded.
• If the resulting average is not within 25 ± 1 Hz, adjust the adjustment
screw in quarter turn increments until the proper tension is read. Then,
repeat the above steps to record ten new readings and calculate the
average.
• Tighten the four screws in the adjustable block. Then, double-check the
belt tension.
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XYZ drive
− Flexi cables
Flexi cable, X-drive

To have access to the flexi cable, remove the top and rear cover.
Flexi cable, Y-drive

To have access to the flexi cable, remove the Y-drive cover.
Flexi cable, Z-drive

To have access to the flex cable, remove the Z-drive covers (top, mid, bottom cover)
and the pipette cover.
Conductive tape will be needed to install the new flex cables.
− Optical Home Sensors
X-Drive
Remove the top cover of the system. The X Opto interrupter is mounted near the
X-axis idler pulley as shown in the figure. It is removed by removing two M3 x 25
button head screws and M3 shakeproof washers.
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XYZ drive
Y-Drive
The Y Opto interrupter is mounted as shown in the figure. To remove the Y sensor,
remove the Y-drive cover and remove the two screws. When you replace the
sensor, make certain that the Y-Flag travels through the center of the sensor.
Z-Drive
The Z Opto interrupter Assembly is mounted on the Z Sensor Plate.
To remove the sensor assembly, remove the Z drive covers, remove the two M3X6
Cap Head Screws and two M3 Shakeproof washers. When you replace the
assembly, ensure that the Z sensor Flag passes through the middle of the sensor.
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Other parts
6.10 OTHER PARTS
• Replacing of the 2 fans on the rear cover
− Remove the top cover and the rear cover to have access to the fans (see
procedure 6.8).
− Disconnect the fan cables from J25 and J26 on the back plane of the system.
− Unscrew the four M3 hex nuts and four M8 shake proof washers on the fan.
− When reinstalling the fan, it should be installed so that the air is forced out of the
interior of the system and the wires are toward the rear.
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Other parts
• Replacing of the interlock switch
The interlock switch is used to prevent operation of the machine unless the cover is
closed or the override key is inserted. The switch is located adjacent to the right
hinge.
− Remove the top cover, the rear cover, the blue system cover and
the right side panel (see procedure 6.8).
− Remove the harness terminals after noting their location.
− Depress the mounting tabs on the sides of the switch to remove it
from the bracket.
• Replacing of the hinges and gas damping spring
These parts are not available as regular spare part. Contact GCS for more
information when these parts need to be replaced. To replace the gas damping
spring a special tool is needed.
Procedure :
Install the spring first on the lower mounting screw and screw the nut a little bit so
that the spring can’t fall down anymore. Now you have to compress the spring so
that it can be installed on the upper mounting screw. By using the washer calibration
tool plate it’s a little bit easier to compress the spring and to put it on the screw. Be
careful, do not bend the spring. If the spring is in the correct position, tighten both
nuts.
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7 C A L I B R AT I O N & V E R I F I C AT I O N
7.1 PIPETTOR
7.1.1 Pipette collar calibration

1. From the software main menu select “Tools”, “System Manual Controls”, and
choose “Drawer”, “Eject“ and click “Do It”.
2. Once the Drawer is ejected, place the pipette collar alignment fixture into the
front-left position. Select “Plate movement”, “Get the Plate from Drawer 1”
and click “Do It”.
3. Observe the pick up of the carrier. Make sure the carrier doesn’t twist when
clamped by the pipette. If it does, then the pipette collar position needs to be
adjusted (see picture).
4. Loosen the 2 small Allen screws (key 1.5 mm) and change the position of the
collar.
5. Tighten the screws. Be careful not to over tighten the screws.
6. After the adjustment repeat the carrier pickup until no skewing is apparent.
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Calibration & Verification
Pipettor
7.1.2 Calibration of the E sensor positions
When the pipette has problems to pickup / eject the plate carrier or the plastic tip,
this calibration procedure might be needed. It defines the position of the plate
sensor and the tip sensor.
The values are used to define if a tip or plate is present.
From the Main Menu, select Tools - Manual Control - Arm - Calibrate E sensor
positions.
The calibration will be done automatically. The following command is performed :
P0SSP - Reply SSP P481,558 T666,743
− Plate sensor position : 481 - 558 (motor steps from home position)
− Tip sensor position : 666 - 743 (motor steps from home position)
The values are stored in the pipette module.
®
Pipettor
7.1.3 Workspace calibration
The workspace calibration is performed to ensure that the coordinates of the

workspace are properly defined :
sample rack pickup

sample positions
control positions
reagent positions
reagent and sample tip pickup
tip release
The workspace calibration will also set the 3 motor encoders to zero. That means
that the workspace calibration has influence on the drawer calibration and the
module plate calibration.The coordinates of the drawer calibration and the plate
calibrations are related to the zero position. A new workspace calibration can result
in a new zero position. So, it’s recommended to repeat the Drawer calibration and to
check at least the module plate calibration. Especially, a deviation in the Y
coordinate can result in plate pickup failures.
The calibrated coordinates are stored on the XY PCB in the EPOD (not on the
computer).
The workspace file (bioMérieux Workspace.wsf) contains all the coordinates of the
sample / reagent / control positions, the sample rack pick up positions, the sample /
reagent tip positions, the deep well plate positions in relation to expected workspace
calibration settings.
This workspace file is defined on a “golden standard instrument” and is the same for
all the instruments.
The computer will calculate a correction factor by comparing the expected

workspace calibration settings (position 2 & 3 on the golden standard) with the
calibrated workspace settings (position 2 & 3 on your instrument). Position 1 is used
to set all the 3 motor encoders to zero.
When the computer has to send a command to the pipettor module to move to a
certain position, the computer will use the correction factor to adapt the fixed
coordinates from the workspace file.
®
Pipettor
To perform the workspace calibration :
1. Remove all components and racks from the workspace area and place the
Calibration Jig (part number WAFIX-003) onto the workspace at the left-hand
end. Make sure that the holes and pins align. The pillars on the plate should
be in the back-left and front-left corners of the workspace area on the
instrument, as shown in the figure.
2. Start the QUATTRO software, select “Tools” and then select “Configure
System”.
Select the “Coordinate Calibration” tab of the Setup System dialog box.
®
Pipettor
3. Press the “Calibrate Workspace” button to start the Workspace Calibration

procedure. The pipettor will move to the home position (left / top) and the
following screen appears.
4. Follow the on-screen instructions to calibrate the workspace. Manually move

the Pipette module to the rear pillar and check that the spigot of the pipette
fits into the divot on the post. Select OK when the arm is in position.
Note: The pipette spigot must enter

the alignment hole vertically
(directly overhead) without any
left-to-right or front-to-back side
movement. Move the x-arm or
y-arm assemblies if necessary
and push on the pipette only
from the top. Check for correct
alignment several times and
hold the pipette spigot in the
alignment hole (without
compressing the spigot spring)
while pressing "OK".
5. The program will alert you that the arm is going to move. Press OK when
ready.
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Pipettor
6. The following screen appears. Manually move the Pipette module to the front
left pillar and check that the spigot of the pipette fits into the divot on the
post. Select OK when the arm is in position.
ready.
8. The program will move the XYZ arm over to the right-hand end of the
instrument to record the position of the right-back pillar. Remove the
Calibration Jig and place it as shown in the next picture.
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Pipettor
9. The following screen appears. Manually move the Pipette module to the back
right pillar and check that the spigot of the pipette fits into the divot on the
post. Select OK when the arm is in position.
ready.
11. This will save the XYZ coordinates to the XY PCB.
NOTE: After defining the front left position and after defining the back right
position, the software will compare the calibrated settings with the
expected settings.
When the difference is too high for one of the coordinates, one of the
following messages will be displayed. Continue or cancel the
calibration and restart the workspace calibration. If not, the wrong
settings will be saved.
®
Pipettor
7.1.4 Drawer Calibration
The drawer calibration locates all of the XYZ positions of the wells of all four plate
positions.
It is performed if a new drawer module is installed, if the tip does not center
correctly, or if the tip does not go to the proper dispense height.
1. Start the DAVINCI QUATTRO software, select “Tools” and then select
“Configure System”.
Select the “Coordinate Calibration” tab of the Setup System dialog box.
2. Press the “Calibrate Drawer” button to start the Drawer Calibration

procedure. Follow the on-screen instructions to calibrate the drawer. The
pipette must be manually moved to the hole in the center of the drawer. The
X, Y, Z coordinate will be saved. Check for correct alignment several times.
Hold the pipette spigot in the alignment hole (without compressing the spigot
spring) while selecting OK.
Example :
X = 7759
Y = 1619
Z = 3420
The coordinates are stored on the Drawer PCB (not on the computer).
®
Pipettor
7.1.5 Module calibration
7.1.5.1 Rough calibration method

Module calibration is done to make sure that the coordinates for each module is
properly defined so that the pipette arm can move the plates as appropriate. The
present position of each module is indicated in the Module Calibration region of the
Setup System dialog box - Coordinate Calibration Tab.
1. Start the QUATTRO software, select “Tools” and then select “Configure
System”.
Select the “Coordinate Calibration” tab of the Setup DAVINCI QUATTRO
dialog box .
2. Select the module to calibrate by left clicking on it and then select “Calibrate
Modules”. The program will prompt you to perform the next steps. The
following is an example using the left front drawer, but all the modules are
calibrated in the same manner.
®
Pipettor
3. Select the “Front Left Drawer” and press “Calibrate Modules”. The drawer will
eject and the arm will position itself over the drawer. The program will prompt
you to insert the Calibration Plate in the front left drawer.
4. Insert the calibration jig and click OK. The drawer will retract and eject again
to ensure proper positioning. The program will prompt you to “Please move
the arm to the front left drawer calibration hole and click OK when done”.
5. Manually move the arm and pipette to the hole of the calibration fixture.
When moving the arm you should always grasp the points at which the arm
and pipette are secured to prevent bending / flexing of the arms. You should
also be cautious not to bump or move the drawer out of its proper position.
Carefully position the tip of the pipette spigot until it fits into the hole of the
Calibration Plate. While holding the pipette in the calibration hole, press OK
when complete. The program will prompt you to “Please remove the
Calibration Plate from the Front left drawer”. Press OK when done.
The drawer will move to the in position.
IMPORTANT! This process must be repeated for every module as required.
The plate coordinates are saved on the Communication PCB in the EPOD
(not on the computer).
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Pipettor
7.1.5.2 Fine tuning using the manual adjustment method

The manual adjustment of a module may need to be performed using a standard
plate carrier if the unit dose not put and get the plates properly. Prior to starting this,
the user should put a standard carrier into the module that requires a manual
adjustment.
1. Start DAVINCI Quattro program, select “Tools” and then select “Configure
System”.
Select the “Coordinate Calibration” tab of the Set-up System dialog box.
2. Select the module to manually adjust by left clicking on it with the mouse and
then select “Manual Adjustment”.
The front left drawer will be used as an example. The other modules would
be adjusted the same way.
®
Pipettor
3. Select “Get Plate” while observing the positioning of the pipette plate clamp
in relation to the carrier. The pipette plate clamp area should pass freely and
evenly into the carrier in each X, Y and Z-axis. If the pipette clamp is not
properly positioned the coordinates can manually be changed by highlighting
the coordinate value in the manual adjustment dialog box and increasing or
decreasing it to realign the pipette clamp. The process may take several
attempts until the optimal results are achieved.
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Pipettor
4. When finished select OK to save the values.

(If you make any changes and exit without selecting OK the values will not
be saved.)
The values in the manual adjustment Dialog Box for the three axes represent
approximately 0.1 mm in travel. The exact Y & Z step count multiplication factor is
0.0625 and the X is 0.0600. The home position for the X is the farthest left position
or 0, the Y is farthest inward position or zero and the Z is the up most position or
zero.
For example the value reported in the figure for Z is equivalent to the step count
(3560), multiplied by 0.0625 would provide the distance in millimeters from the Z
home position
(= dividing the number of motor steps by 16).
Depending on the particular situation you can either increase or decrease the
number to achieve the optimal position. Try small increases or decreases around 5
until you gain a thorough understanding.
After calibrating the workspace, drawer or modules perform the Cycle Test
and the Tip Check Test.
®
Washer
7.2 WASHER
7.2.1 Calibration of the Vacuum Sensor
CAUTION! Ensure the waste bottle is empty and the wash head is present and
connected.
• Remove the reader module to have access to the vacuum switch.

• Connect manometer and voltmeter as depicted below. The manometer is
connected in-line with the vacuum switch using a tee and 2 short lengths
of tubing. To do so, remove the vacuum switch (it’s not necessary to
unscrew the 2 allen screws completely).
• Turn on the instrument and initiate the software.

• Choose “Manual Control” from the Tools menu.
• Choose “Advanced” - “Send Command(s)”, type W0STS and click “Send”, this will
self-test the washer. Next type W0APCY and click “Send”, this will turn on the
aspirate pump. Turn on both meters and ensure the waste bottle cap is tight. The
manometer should read a vacuum of 0.60 psi (31 mmHg), or higher (normally around
1.15 psi). If not, stop and inspect for leaks.
• Reduce the vacuum by pinching off the vacuum supply tubing (this is the left tubing of
the waste bottle) until the manometer reads steady vacuum of 0.43 - 0.47 psi
(22,24 - 24,31 mmHg). The voltage should switch from 0 Vdc to 5 Vdc at this point.
If not, tweak the vacuum sensor adjustment screw so that it switches at this setting
(clockwise to lower set-point - 1/8 turn = 0.05 psi).
• When complete type W0SHT and click “Send”, this will turn off the pump.
®
Washer
7.2.2 Calibration of the Dispense Pump Pressure
This calibration is done by the pump manufacturer. It needs not to be done in the
field. However, when the dispense pump can not prime empty lines, the pump can
be calibrated according to this procedure.
Tools / Parts needed:

Smart manometer (ref. code 399129-901 or locally)
Wrench nº 13
4 mm allen wrench
Silicon tubing (3 mm ID) - 1 meter
T-connector
Phillips screwdriver
Checking the Pressure Setting

1. Remove the left side panel (4 screws). Remove the KNF Dispense Pump
Mount with pump from the left side enclosure (2 screws).
®
Washer
2. Attach a manometer (positive pressure) to the dispense pump. See diagram

below.
3. Ensure Wash Container A is filled with liquid (washbuffer or distilled water).

In the daVINCI QUATTRO software, go to Manual Control - Advanced -
Send command(s).
Prime line A by using the command W0PURA9999. This command will
switch on the dispense pump and the aspirate pump. It will prime channel A
with 9999 µl per needle.
After priming type the command W0SHT to switch off the aspirate pump.
NOTE: Take caution as the fluid will want to travel up the tubing to the
manometer and this may damage the manometer. You will want to
pinch the silicon tubing to the manometer when the pump is first
turned on and then slowly let it go. Place the manometer higher than
the pump.
To prime the line if the dispense pump is not working at all, you can
connect a large syringe to the outlet of the dispense pump. Using the
syringe, you can support the dispense pump in sucking up the liquid
from the container.
4. When the system is primed properly, ensure the manometer and the tubing
are connected according the diagram above. Turn the dispense pump on
using the command W0DPCA250. The dispense pump will run continuously
with maximum speed. The pinch valve at the wash head will stay closed. The
pressure inside the lines is monitored by the manometer.
5. The PSI reading should be between 3.5 and 4.235 PSI. If it needs adjusting,
see instructions below on how to change the pressure setting.
6. Turn the dispense pump off by sending the command W0DPCA000.
Do not leave the pump on for a long time.
7. Reinstall the dispense pump mount into the left side enclosure. Reinstall the
left panel.
®
Washer
Changing the pressure setting

1. Using a wrench (nº 13), loosen the 13 mm silver lock nut at the top of the
dispense pump.
2. With the 4 mm allen wrench, rotate the top screw clockwise until the
manometer reads approximately 4 PSI (turning clockwise will increase the
pressure).
3. Tighten the lock nut.
®
Barcode Scanner
7.3 BARCODE SCANNER
7.3.1 Selection of the barcode label type

Before verifying and adjusting the horizontal and vertical barcode scanner, ensure
the used barcode labels are enabled.
− With DAVINCI QUATTRO Software open, go to Tools - Configure
System - Sample ID.
− Select the available Barcode Scanners :
• Horizontal Barcode Scanner (plate barcode scanner)
− Select the tab of barcode label type and verify if this type is enabled.
®
Barcode Scanner
• Vertical Barcode Scanner (sample barcode scanner)
− Select the tab of barcode label type and verify if this type is enabled.
®
Barcode Scanner
7.3.2 Verification of the sample barcode scanner

• Insert bar-coded sample tubes in the first and seventh sample rack.
• With DAVINCI QUATTRO Software open, go to Tools and System
Manual Control.
• From the left hand list select Sample ID.
• From the right hand list select Scan Sample Rack 1 and click on Do It.
• Once that is complete select Scan Sample Rack 7 and click on Do It.
If the barcodes were read, the rack will be put back in the caddy.
The results are not displayed.
If an error occurs during scanning, you will get a System Sample ID
Error. Verify that the barcode is facing the SID and then select
Recovery Options and choose Try to Rescan this Barcode. You
may also choose the Move This Barcode Slightly and Try to
Rescan. If the error persists, select Recovery Option and choose
Skip Reading This Barcode and continue.
The system has a search algorithm that will move the rack back and forth to locate
the barcode. If all sample barcodes cannot be read verify if the type of barcode label
is enabled. If using a correct barcode label then an adjustment must be made.
7.3.3 Verification plate barcode scanner alignment

• With DAVINCI QUATTRO Software open, go to Tools and System
Manual Control.
• From the left hand list select Drawer.
• From the right hand list select Eject.
• Insert bar-coded plates into the first and fourth positions, ensuring the
barcodes are on the long side of the plate, facing the SID Module.
• Still in manual control, select Sample ID.
• Choose Scan X Plate in Drawer 1 (X is the location of the barcode on the
plate. Left, Center or Right.) Click on Do It.
• After the first plate is complete, Choose Scan X Plate in Drawer 4 and
click on Do It.
If the barcode was read, the plate will be put back in Drawer 1. The
result is not displayed.
If the barcode is not read, verify that the barcode is facing the
barcode scanner and then select Recovery Options choose Move
This Barcode Slightly (the plate will move some steps downwards)
and Try to Rescan. If the barcode is still not read, take note of
where the plate/barcode are lined up with the SID and make
adjustments.
®
Barcode Scanner
7.3.4 Adjustment of the sample / plate barcode scanner

The sample barcode scanner has been adjusted by the manufacturer. The correct
settings can be found on a form included with the instrument (scanner).
For the plate barcode scanner the default settings can be used.
In case the settings are not giving the correct readings, perform the following
adjustments.
a) Select “Tools”, “Configure System”, click on the “Sample ID” tab.
b) Record the default Sample Rack Scan Position, Plate Scan Position and L/R Pos
Offset numbers. Next make any adjustment in either X, Y or Z.
− By decreasing the Y value the beam length in the Vertical direction
will decrease, by increasing the beam it will lengthen and loose
focus.
− Decreasing the Z value will raise the reading position of the rack,
increasing the number will lower it.
− Increasing the X value will move the rack to the right, decreasing it
will move it towards the left.
In most cases the Y and Z values for the sample barcode scanner will provide the
broadest coverage and an adjustment in the X will be all that’s needed.
The Plate Scan position is adjusted in the same manner with the likely adjustment
being in the Z direction. The L/R position offset determines the number of steps to
move off center to read plate barcodes that are placed to the left or right.
Default = 538 steps (538 x 0.0600 = is 32.28 mm out the center)
Once the adjustment is made repeat the verification steps.
®
8 E S P ( E L E C T R O N I C S I G N AT U R E P I P E T T I N G )
8.1 ESP DESCRIPTION
The pipetting system of the DAVINCI QUATTRO instrument includes ESP software
for automatic detection of clots, foam, bubbles or low volume when pipetting
samples.
ESP contains an aspirate check and a dispense check.
The detection is done with a pressure sensor, which gives an analogue signal
dependant upon the pressure inside the barrel of the pipette.
The pipetting signature observed when pipetting the sample, is compared to nominal
pipetting signatures obtained during system configuration. If the pipetting signatures
does not fall within the normal range, the system reports an error.
The emphasis has been put on identifying good valid samples rather than bad
samples.
A sample may fail but nothing has been done to identify why it has failed.
The performance of ESP is reliant on consistent viscosities of sample fluids, which

may or may not work reliably in routine use and is dependant on consistent sample
batch characteristics.
75 samples are needed for teaching.
The minimum sample volume for ESP is 5 µl. However, a fail rate of 10 % has been
observed for 10 µl. For the customer it might be not convnient to use ESP with
volumes lower than 50 µl.
A curve must be defined for each sample volume.
The pressure is measured 3 times per µl of samples.
ESP will report a volume failure when the volume is 9% to 13.55% lower. There is
no overlap in curves for 50 µl and 100 µl.
The pressure curve depends on tubing / sensor / type of sample : serum - plasma /
volume / viscosity of the sample / dispense profile (use always 4).
®
ESP (Electronic Signature Pipetting)
ESP description
The shape of the pressure curve during aspiration is shown below.
A : sample volume in steps
B : varies with final speed, tip

and sample volume
C : varies with viscosity, ramp

rate and tip size
D : varies with density
Assuming that only the sample volume changes, A and B will change. This will bring
an obvious change in the area under the curve. This shape is modified with an
oscillatory waveform caused by the spring damper effect of the air column. The
shape and probably area will be modified further by any transients caused by
bubbles or clots interfering with the flow of medium into the pipette tip. Given that
the shape and area for a given sample size are known then any changes from this
ideal pattern can be detected.
The following information is sent by the pipette module and are used to define
several algorithms to evaluate the sample :
Area : The pipette pressure is measured 3 times per µl of sample. The area under
the curve, the integral, can be calculated. In order to remove any contamination of
the result by any bias of the signal towards positive or negative pressure (an offset),
the first pressure reading is subtracted from all subsequent ones. The area value of
7 digits is sent by the pipette module.
Curve Length : This is the perimeter length of the curve (6 digits). It can easily be
calculated by adding the absolute differences between consecutive pressure
readings.
Area Peak : For a curve there is a maximum pressure point theoretically as the
deceleration ramp starts. Any bias has been removed. (3 digits)
Pressure before air gap (4 digits) and pressure after the air gap (4 digits) is also
defined during the aspirate step.
The final column pressure (4 digits) is the first pressure before dispensing.
NOTE: When viewing the ESP detection data, the “area length” is also
displayed.
This value is a calculation of the Area divided by the curve length.
This value is not sent by the pipette module. The ratio varies
proportionally to the shape of the area.
®
ESP description
The signal from the pressure sensor as received by the ADC is from 0 to 5 volts.
Where 2.5 volts represents zero pressure, while those above and below represent
positive and negative pressure respectively. The output from the pressure sensor
has been filtered, amplified and level changed to achieve this. The gain can be
dynamically changed under software control. The gain depends on the volume and
is defined during the ESP calibration. The gain will be lower when the volume is
higher. The aspirate needs a higher gain than the dispense.
The ADC gives a 10 bit output allowing values from 0 to 1023. The half (511)
represents zero pressure. In order to protect the pressure sensor from excess
internal pressure, the pipette is stopped in its movement if the ADC output exceeds
1000 or reduces to 0.
The ESP calibration can be de-sensitized by increasing the threshold value. A

correct set up can greatly reduce the amount of false ESP errors.
An ESP error (e.g. clot) might result that the sample (+ clot) will be dispensed back
into the sample tube rather than risk dragging the clot all around the workspace.
examples
®
ESP description
Examples :
10 µl good sample
Aspirate : ASP0276,000507825400028704060389
These values are sent from the pipettor to the computer to evaluate the sample.
0276 = Motor steps, position of P motor.
0005078 = The summation of all pressure readings during the aspirate. The pressure
is measured 3 times per µl sample (in this example 30 times). This value is
called the area.
254 = The minimum / maximum pressure during the aspirate. This is called the
area peak.
000287 = The summation of the absolute difference between consecutive pressure
readings for all pressure readings during the aspirate. This is called the
curve length.
0406 = The pressure before the air gap move.
0389 = The pressure after the air gap move.
Dispense : DIS0100,00271673090007080444
0027167 = The summation of all pressure readings during the dispense. The pressure
is measured 3 times per µl sample (in this example 30x). This value is
called the area.
309 = The minimum / maximum pressure during the dispense. This is called the
area peak.
readings for all pressure readings during the dispense. This is called the
curve length.
0444 = The first pressure reading.
Examples :
10 µl foam sample
Aspirate : ASP0276,000091604400017304670413
These values are sent from the pipettor to the computer to evaluate the sample.
0000916 = The summation of all pressure readings during the aspirate. The pressure
is measured 3 times per µl sample (in this example 30 times). This value is
called the area.
044 = The minimum / maximum pressure during the aspirate. This is called the
area peak.
readings for all pressure readings during the aspirate. This is called the
curve length.
0467 = The pressure before the air gap move.
0413 = The pressure after the air gap move.
®
Software limitations
8.2 SOFTWARE LIMITATIONS
• Low volume samples (e.g. 10 µl), the fail rate is about 10%.
• The ESP database file is needed to link an assay to the ESP curve in
demo mode.
®
9 F I R M WA R E U P G R A D E
9.1 INSTALLATION OF FLASH PROGRAM
Unzip the DSXFlashSetup.zip file.

Install the program by selecting the DSXFlashSetup.exe file.
Follow the instructions on the screens (Click Next - Next - Next -
Next - Finish) as shown below.
®
Firmware Upgrade
Procedure to flash the firmware
9.2 PROCEDURE TO FLASH THE FIRMWARE
The following modules must be flashed:

Module Minimal
Version
Communication module 1.02
Absorbance reader 1.21
Drawer/SID module 2.20
Washer module 1.15
Incubator module 2.09
Pipette module 1.05
XY module 1.02
IMPORTANT! Check for the files latest update on the GCS portal.
9.2.1 Flash the Firmware of the Communication (EPOD) or

Pipette modules.
NOTE: these two firmware are downloaded into the modules thanks to Auto-
executable programs. The procedure below applies to both .exe files.
• Download the current “Communication FW Installer” or “Pipette FW
Installer” file from GCS portal.
• Copy these files onto a computer attached to the DaVINCI QUATTRO
instrument.
• From Windows Explorer, run the appropriate Installer program
(Communication or Pipette).
• Wait until the operation is completed
9.2.2 Flash the Firmware of the Incubator modules.

NOTE: It is mandatory that all Incubators modules are holding the same
firmware versions, when installed together in a DAVINCI QUATTRO.
When replacing one module, pay attention that this rule is applied.
This firmware is downloaded into the incubators modules thanks to an

Auto-executable program.
• Download from GCS portal the current file called “Incubator FW Installer”.
• Copy this file onto a computer attached to the DaVINCI QUATTRO
instrument.
• From Windows Explorer, run the Incubator FW Installer program.
• When prompted, select the incubator which firmware needs to be updated
in the pull down list.
• Wait until the operation is completed.
®
Firmware Upgrade
9.2.3 Flash the Firmware of the Drawer / SID, Reader,

Washer or XY board modules.
• From a computer attached to the DaVINCI QUATTRO instrument, run the
current “DaVINCI QUATTRO DSXFlashSetup” program.
The window below appears :
• In the “Select Module to Upgrade” pull down list, select the module to be
updated, among the bold ones below :
port 00 C0 (communication module DO NOT SELECT)
port 01 -
port 02 -
port 03 D0 (drawer)
port 04 S0 (barcode scanner) =SID
port 05 I1 (incubator 2 DO NOT SELECT)
port 07 A0 (reader)
port 08 W0 (washer)
port 11 -
port 12 -
port 13 P0 (pipette DO NOT SELECT)
port 14 X0 (XY module)
port 15 -
Note: The firmware of the modules flagged DO NOT SELECT can be updated thanks to
executable files, see sections 9.2.1 and 9.2.2.
®
Firmware Upgrade
WARNING! The module selected by default is the Communication module.

Whenever another firmware is downloaded into this module, the
communication will be lost, and the only way out is to replace the
chip in the EPOD with S/P ref. 4568858400, in order to restore the
communication with the computer.
• Select the correct “Upgrade Filename” e.g. FW Reader AM1v21 (Reader
Firmware version 1.21).
• The module location is “Attached to DSX” and the COM port is usually
“COM1” (default selection).
• Select the “Program” button to start the upgrade. The boot program is
loaded (few seconds) and then the firmware is downloaded (5 minutes).
• Once the update is completed, you are prompted to recycle the power of
the DaVINCI QUATTRO instrument. Once you have recycled the power,
click “OK” in window below :
• When back in the “DSX Flash Updater” window, click “Close” (or proceed
to next update).
®
Firmware Upgrade
Firmware troubleshooting
9.3 FIRMWARE TROUBLESHOOTING
When downloading firmware to a module, you have always to select the correct
module. However, by mistake it can be possible that the wrong module was
selected.
e.g. drawer firmware was downloaded to the reader
This is not a problem. In this case you can always download the reader firmware to
the reader again.
But, be careful. When the wrong firmware was downloaded to the Communication
PCB, the communication between the instrument and computer will be lost after the
download. Downloading firmware is no longer possible.
What to do when the wrong firmware was downloaded to the communication

PCB.
The IC component containing the firmware is the same for all modules. Only the
firmware is different. So, replacing the firmware component will resolve the problem.
1. You can order the IC, firmware for the communication PCB – spare part
reference 45688584
2. Cheaper and faster, use a second instrument and downloading the
communication firmware to one of the other modules. Preferable, choose
incubator 1 (= I0).
Select the communication firmware file and select "port 10 - I0" as module
to upgrade.
After the download, remove the IC firmware component from the incubator
PCB.
Install it on the communication PCB.
Change the label.
®
Firmware Upgrade
Firmware troubleshooting
Afterwards, you have to install the IC coming from the communication PCB
(instrument nº 1) into the Incubator module (instrument nº 2) and you have to
download the incubator firmware to this module. The incubator module will not be
recognized by the flash program when selecting the “MAP” button. But, this
unrecognized module is located on Port 10. This is the normal location for incubator
1 (= I0). To program the incubator firmware, select port 10.
NOTE: Remember when one of the incubators is not recognized e.g. in this
case incubator 1, incubator 2 becomes I0, incubator 3 becomes I1
and incubator 4 becomes I2. When pressing the MAP button, you will
found I0, I1 and I2.
Troubleshooting : You can use also this method when you can not download the
firmware to a module for any reason.
e.g. downloading new firmware to the washer is not possible
download the washer firmware to incubator 1 and replace the IC’s
®
10 PREVENTIVE MAINTENANCE
10.1 OPERATOR MAINTENANCE
The operator maintenance is summed up in the table below. If necessary, refer to

the Operator Manual, Chapter 8.
Description of Maintenance in the Operator Manual Page
General information about maintenance
Required Materials 8-2
Maintenance Schedule 8-3
Operator Maintenance Actions
Daily Maintenance 8-4
Bi-Weekly Maintenance 8-7
Monthly Maintenance 8-8
Maintenance as Required 8-8
®
Preventive Maintenance
Service Preventive maintenance
10.2 SERVICE PREVENTIVE MAINTENANCE
It’s recommended to perform the preventive maintenance on a six month basis.

Additionally to the tools needed for installation and service of the instrument (see
chapter 2 - Instrument Installation), the following items are needed to perform
preventive maintenance.
• Non-Abrasive Cleaner
• Isopropyl Alcohol 70 % + Mucasol 3% solution or Extran
• Foam or cotton tip swabs
• Loctite 222
• Consumables to run the Cycle Test - 2 :
− 2x 35 ml Bottles low density solution
− 1 L Distilled water
− 1x Break-4 plate
− 1x Deep well plate or 12x Deep well dilution strips
− 20x Control bottles
− 2x Reagent bottles
− 108x Sample tips and 41x Reagent tips
− 8x Sample tubes
Tools needed:
− Metric Allent kit set 1.5-6 mm
− Voltmeter
− Smart manometer (ref. code 399129-901 or locally)
Spare part Article code Number of pieces
Dispense Tubing Maintenance Kit 45688530 1
Aspirate Tubing Maintenance Kit 94568852 1
Air filter, outlet 45688581 1
Air filter, reader 7513060517 1
Every 3 years the lamp in the reader module must be replaced:

Lamp, 6 V - 20 W 45688527 1
Some other parts might be needed during preventive maintenance:

Dispense Tubing Maintenance Kit 45688509 1
Setscrew 45688550 1
Metal clip with dimple 45688586 1
Metal clip without dimple 45688585 1
®
Preventive Maintenance procedure :
1. Power up the DAVINCI QUATTRO and start the DAVINCI QUATTRO

Software. View the self-test and ensure all tests pass within specifications.
Exit the software, switch off the power to the instrument and unplug the
power cord.
2. Remove all consumables and racks from the workspace. Empty the waste
bottle and tip tray into a suitable Biohazard collection vessel.
3. Decontaminate using 70% or greater Isopropyl alcohol and spray all
surfaces, plate carriers, pipette spigot, module plate carriers.
4. Clean all surfaces using an all-purpose cleaner.
5. Remove the Reader module and place on flat, safe surface. Remove the
filter cover in the back of the module. Using plastic tweezers, remove each
filter one at a time and inspect for clouding and scratches. Clean with a lent-
free cloth. Install the filter back into the filter wheel before removing the next
filter. Moisture-free air can be used to remove dust. Use a lent-free cloth to
remove dust from the bulb and lens under the bulb. Do not touch the bulb
with bare hands. After all filters are checked, install the filter cover. Reinstall
the reader module.
6. Remove the Covers from the X-Axis, Y-Axis, Z-Axis and Pipette.
• Visually inspect each of the drives for wear and excessive build up of
debris. Clean as required. Make note on service report if large amounts
of debris are found near any pulleys.
• Ensure the z-drive counter-balance spring winds / unwinds without
binding.
• Inspect the grounding tape and ensure it is tightly stuck in place. Replace
as required.
• Check the tightness of the X, Y and Z pulleys setscrews. If the setscrew
can be tightened, discard the screw and replace with a new setscrew
using loctite 222.
7. Using a digital multimeter, measure the resistance between the Pipette

bottom plate (cover mounting holes) and the EPOD module ground pin. The
reading should be lower than 2.5 Ω. If not, the grounding tape on the X, Y
and Z cables should be checked for proper adhesion. Replace the tape, if
needed.
Resistance = ____________ Ω (less than 2.5 Ω)
®
8. Inspect the Pipette Collar Mechanism for wear and debris between Tip Body
and Clamp Body. Clean the Pipette collar Mechanism using the cotton-tipped
applicators and isopropyl alcohol. Do not apply alcohol to the lead screws of
the two motors.
To clean the tip body, move the E motor down by means of the following command :
P0MMAE01000
®
9. Clean the dispense tip. If dirty, use a small cleaning wire to clean it out.
10. Reinstall the covers for the X, Y and Z drives and the Pipette module. Install
the Z-arm middle cover first, ensuring the ferrite core is still next to the
connector at the top of the Z-transition PCB when the cover is in place.
11. Remove and insert all modules again to have good contacts.
12. Inspect the spring clips of the 4 microplate holders for damage. Replace,
repair as necessary.
− 45688586 metal clip with dimple

− 45688585 metal clip without
dimple
®
13. Remove the 8 way wash head, submerge it in 70% or greater Isopropyl
alcohol and clean both aspirate and dispense pins with cleaning wires. Then
remove the four cleaning port setscrews on the wash head. Flush with DI
water. When finished, install the four setscrews. Do not over-torque these
screws because the wash head can be cracked easily.
Cleaning
port screws
14. Using the tubing diagrams on the following pages, assemble the replacement
tubing for both dispense and aspirate tubing. Leave the 4 reducer fittings, 42
mm PVC tubing and elbow fittings off the dispense lines until assembled in
the front extrusion.
Tubing T1 (aspirate) is normally not replaced during the PM can be used for the
waste container
15. Using the newly-assembled tubing, replace the existing tubing and reconnect
it exactly as removed. Removing the Washer module will help facilitate the
replacement of the vacuum switch tubing. Use existing clips to secure tubing
and secure it is not pinched or compressed in any way that would impede
flow. Replace any unsecured or damaged clips. Reinstall the Washer module
if removed. Reinstall all panels and ensure no tubes are pinched off.
®
Aspirate Tubing Maintenance Instructions
1. Replace the tubing and related parts using the kit and the diagram below.
2. For units with a vacuum trap, do the following:
a. Cut the 300 mm Tubing (T1) to 220 mm.
b. Switch the positions of the 220 mm Tubing (T1) and the 650 mm Tubing (T2).
c. Connect the Vacuum Trap in the position of the Straight Fitting (F1).
d. Discard the Straight Fitting (F1).
Since July 2006 the red vacuum relief valve is

removed from the aspiration tubing maintenance kit.
®
Dispense Tubing Maintenance Instructions
Instructions : Replace the tubing and related parts using the kit and the diagram
below.
®
16. Replace the air filter (outlet aspirate pump).

17. Inspect all o-rings on the bottle fittings, replacing any that are cracked or
deteriorated. Apply silicone grease to the o-rings and ensure the fittings are
easily fitted into the bottles.
18. Remove the reader module to be able to adjust the vacuum switch in the
next step. Reconnect the power cord. Switch the power on to the instrument
and start the DAVINCI QUATTRO Software.
19. Select “Tools” – “System Manual Control” and perform the following
operations :
• Connect manometer and multimeter as depicted below. The manometer is
connected in-line with the vacuum switch using a tee and 2 short lengths
of tubing. To do so, remove the vacuum switch (it’s not necessary to
unscrew the 2 allen screws completely).
• Choose “Advanced” - “Send Command(s)”, type W0STS and click “Send”,

this will self-test the washer. Next type W0APCY and click “Send”, this will
turn on the aspirate pump. Turn on both meters and ensure the waste
bottle cap is tight. The manometer should read a vacuum of 0.80 psi (31
mmHg), or higher (normally around 1.15 psi). If not, stop and inspect for
leaks.
Record this maximum pressure value _______ (> 0.80 psi / 31 mmHg).
• Reduce the vacuum by pinching off the vacuum supply tubing (left tubing
waste bottle) until the manometer reads steady vacuum of 0.43 - 0.47 psi
(22,24 - 24,31 mmHg).
The voltage should switch from 0 Vdc to 5 Vdc at this point. If not, tweak
the vacuum sensor adjustment screw so that it switches at this setting
(clockwise to lower set-point - 1/8 turn = 0.05 psi). Record the pressure
value (trigger point) _______ (0.43 - 0.47 psi).
• When complete type W0SHT and click “Send”, this will turn off the pump.
®
• Switch off the instrument. Disconnect the manometer and multimeter and
reassemble the tubing and sensor back into the system. Visually check
that the vacuum sensor is tightly secured to the sidewall. Check for kinks
or pinching of the tubing.
20. Re-install the reader module.

21. Do a final inspection to ensure everything is re-assembled.
22. Switch on the power to the instrument and start the DAVINCI QUATTRO
Software. Print the self-test report and check for failures. If any errors are
present stop and correct the problem.
23. Check all modules for the latest firmware version. Update, if needed.
24. From the software main menu select “Tools”, “System Manual Controls”, and
choose “Drawer”, “Eject“ and click “Do It”. Once the Drawer is ejected, place
the pipette collar alignment fixture into the front-left position. Select “Plate
movement”, “Get the Plate from Drawer 1” and click “Do It”. Observe the pick
up of the carrier, make sure it doesn’t twist as it is clamped by the pipette. If it
does, then the pipette collar needs to be adjusted. If adjustments should be
made, then repeat the above until no skewing is apparent.
25. Remove sample tubes and the control rack otherwise the tips might hit the
tubes and bottles during the next test. Select “Tools”, “System Manual
Controls”, “Testing”, and “Tip Alignment Test” and click “Do It”. Observe the
movement and ensure the tip is properly positioned into each area. A
workspace calibration needs to be done if there is a problem with the tip
pickup. Then, run the Tip Alignment test again. Exit the program. (if a
workspace calibration is performed, the drawer calibration must be repeated
and all module coordinates must be rechecked)
®
26. Run the WASHDISP assay to fill a microtitre plate with 300 µl DI water to run
the Plate Movement test. Check the dispensed volume. Clean the wash head
if necessary.
27. Select “Tools”, “System Manual Controls”, “Testing”, and “Plate
Movement”.Click “Do It”. Enter cycle count of 12 and place a carrier with the
filled microtitre plate into the #1 ambient drawer position. While running the
test, watch the plate pick-up at each position. If necessary, choose “Tools”,
“Configure System” and manually adjust the module for proper pick-up.
28. Perform the Instrument qualification.
NOTE: During the verification, check for leaks in the installed dispense
tubing. Check and make sure that the head is not leaking from the
cleaning ports.
29. Reinstall the cover lock key and ensure the lock works. From the software
select “Tools”, “System Manual Controls”, “Arm”, “Lock cover” and “unlock
cover”. If it is not working properly adjust the cover position. Loose the 6
screws of the cover and push the cover up. Then, tighten the 6 screws.
Repeat the test.
30. Remove all consumables used for the test and clean the workspace.
31. Delete all the log files from the folder
C:\ Program Files \ bioMérieux \ DAVINCI QUATTRO Program \ Logs
NOTE: Whenever a complete verification is required, run the Burn in and

ensure results indicate a passed value.
®
DAVINCI QUATTRO Preventive Maintenance Checklist
Item Done Printout

MAINTENANCE
Clean and decontaminate the system. -
Clean the filters in the Reader. -
Check the X, Y, Z movement. -
Measure the grounding of the pipettor : Resistance = ______ Ω (R < 2.5 Ω) -
Clean the pipette Collar mechanism and the dispense tip. -
Inspect the metal spring clips of the 4 plate carriers for damage. -
Clean the wash head. -
Replace the aspirate tubing. -
Replace the dispense tubing. -
Replace the air filter (outlet aspirate pump). -
Vacuum switch : check the trigger point (calibrate if needed)
Maximum Pressure = _________ (higher than 0.80 psi) -
Pressure (trigger point) = __________ (0.43 - 0.47 psi)
Check the DAVINCI QUATTRO Self Test Results. Y
Check all modules for the latest firmware version. -
Check the cover lock.
Check the Pipette Collar alignment. -
Delete the log files and the bioMérieux text files from the hard disk (see with the
user).
-
MECHANICAL TESTS A
Run the Tip Alignment test. -
Run the Plate Movement test (36 cycles – microplate filles with 300 µl). -
QUALIFICATION TESTS
Perform the Reader Verification (OPSYS + C2C).
Perform the Calibration Plate DRP.
Perform the Pipettor Verification.
Perform the Pipette volume calibration (if pipettor verification is not conform).
Calibrate the Wash Head Offsets.
Perform the Washer Verification (dispense volume + residual volume).
Perform the Washer Pump calibration (if washer verification is not conform).)
Run the Incubator Verification (INC 1 – INC 2 – INC 3 – INC 4). Y
Compile results in Dprog Y
Close the Engineering mode -
........................................................................................................................
Notes / Comments :
........................................................................................................................
Performed by: ............................... Date: ................. Instrument Serial Number ......................................

11 S PA R E PA R T S L I S T
Local stock 1-5 : The number of parts that is recommended to have on stock when
having installed 1 to 5 instruments.
11.1 SPARE PARTS
ART. NO. DESCRIPTION REPAIRABLE LOCAL STOCK 1-5
45688581 AIR FILTER, OUTLET NO 1
7513060517 AIR FILTER, READER NO 5
45688502 ASSY, BARCODE SCANNER YES
7513060540 ASSY, BLUE COVER NO
45688501 ASSY, DRAWER YES 1
7513060603 ASSY, EPOD NO
45688503 ASSY, INCUBATOR YES 1
45688504 ASSY, PIPETTE YES 1
45688500 ASSY, READER YES 1
45688505 ASSY, WASHER YES 1
45688506 ASSY, Z DRIVE NO
45688507 BEARING-BALL, Y DRIVE NO
45688508 BELT, X DRIVE NO
200418 COMPUTER + KEYBOARD + MOUSE NO
45688509 CONDUCTIVE TAPE NO 1
45688510 CONNECTOR, 4 POLE FEMALE SOCKET NO 1
45688511 COUPLING, ELBOW, WASH BOTTLE (4X) NO 1
45688582 COUPLING, FEMALE, LARGE, WASTE NO 1
45688583 COUPLING, FEMALE, SMALL, WASTE NO 1
45688512 COUPLING, LARGE ELBOW, WASTE NO 1
45688513 COUPLING, SMALL ELBOW, WASTE NO 1
45688514 ENCODER FOR X-Y-Z DRIVE MOTOR NO
45688515 FAN, EPOD - 24V NO
45688516 FAN, READER MODULE NO
45688517 FAN, SYSTEM NO
45688518 FILTER, 405 NM NO
45688519 FILTER, 450 NM NO
45688520 FILTER, 490 NM NO
4568858700 FILTER, 570 NM NO
®
Spare parts list
Spare Parts
45688580 FILTER, 610 NM NO
45688521 FILTER, 620 NM NO
45688522 FILTER, 690 NM NO
7438023167 FITTING, LEFT, WASTE CONTAINER NO 1
7438023168 FITTING, RIGHT, WASTE CONTAINER NO 1
45688523 FLEXI CABLE, X DRIVE NO
45688524 FLEXI CABLE, Y DRIVE NO
45688525 FLEXI CABLE, Z DRIVE NO
7513060518 GAS DAMPING SPRING, COVER NO 2
45688526 HEAD ASSEMBLY, 8-WAY, WASHER NO
4568858400 IC, FIRMWARE COMMUNICATION PCB NO 1
45688527 LAMP, 6V - 20W NO 1
45688528 MAINTENANCE KIT, ASP PUMP NO
45688529 MAINTENANCE KIT, ASP TUBING NO 1
45688530 MAINTENANCE KIT, DISP TUBING NO 1
4568858500 METAL CLIP WITHOUT DIMPLE, PLATE CARRIER NO 10
4568858600 METAL CLIP WITH DIMPLE, PLATE CARRIER NO 10
45688531 MICROSWITCH, WASH X / WASH Z NO 1
93443 MONITOR, 17 INCH TFT NO
45688534 MOTOR, X DRIVE NO
45688535 MOTOR, Y DRIVE NO
45688536 MOTOR, Z DRIVE NO
7438023166 O-RING, FITTING, WASTE CONTAINER NO 2
7513060602 PCB, BACK LANE NO
45688538 PCB, COMMUNICATION NO 1
45688539 PCB, X TRANSITION NO
45688540 PCB, X/Y DRIVE NO 1
45688541 PCB, Y TRANSITION NO
45688542 PCB, Z TRANSITION NO
45688543 PINCH VALVE, WASHBUFFER BOTTLE NO 1
45688544 PINCH VALVE, WASHER NO 2
45688545 POWER SUPPLY, 600 W NO
99223 PRINTER, LEXMARK E352DN, 220 V NO
99393 PRINTER, LEXMARK E352DN, 110 V NO
45688547 PUMP, DISPENSE (TYPE NF 30) NO 1
45688548 PUMP, VACUUM, 12VDC NO
45688549 SENSOR, OPTICAL NO 2
45688550 SETSCREW, M4X6 NO 1
®
Spare parts list
Tools
45688551 SILENCER, VACUUM PUMP NO
45688552 SPRING, COUNTER BALANCE, PIPETTE NO
45688553 SPRING, PLATE CARRIER WASHER NO 1
45688554 SWITCH, COVER INTERLOCK NO
45688555 VACUUM FILTER, IN-LINE NO 1
45688556 VACUUM RELIEF VALVE NO 1
45688557 VACUUM SWITCH NO 1
45688558 VACUUM TRAP NO 1
45688559 X HOME SENSOR, X DRIVE NO
45688560 Y HOME SENSOR, Y DRIVE NO
45688561 Z HOME SENSOR, Z DRIVE NO
11.2 TOOLS
ART.NO. DESCRIPTION REPAIRABLE LOCAL STOCK 1-5
45688562 CABLE, EXTENSION, READER NO 1
45688563 CALIBRATION PLATE, MODULE NO 1
45688564 COLLAR ALIGNMENT PLATE CARRIER NO 1
45688565 CLEANING REAMER, PIPETTE (6X) NO 1
280103 DIAGNOSTIC KIT (LIQUIDS) NO 1
45688566 FIXTURE, PIPETTE ALIGNMENT NO 1
45688569 FIXTURE, WASHER ALIGNMENT NO 1
45688567 FIXTURE, WORKSPACE CALIBRATION NO 1
284119 INCUBATOR VERIFICATION TOOL Calibration 1
45688568 READER VERIFICATION PLATE Calibration 1
45688579 DYNEX SERVICE MANUAL NO
45688570 TOOL, LOW-SIDED VESSEL (5X) NO 1
278303 UNCOATED MICROPLATE (100 PCS) NO 1
45688571 WIRE, CLEANING, ASP NEEDLES NO 1
45688572 WIRE, CLEANING, DISP NEEDLES NO 1
399129-901 SMART MANOMETER YES 1
®
Spare parts list
Accessories
11.3 ACCESSORIES
ART.NO. DESCRIPTION REPAIRABLE LOCAL STOCK 1-5
7513060542 BASE PLATE, DEEPWELL NO
272240 BOTTLE, CONTROL (344X) NO
45688575 CADDY, SAMPLE RACKS NO
272241 CAP, CONTROL BOTTLE (2000X) NO
45688573 CONTAINER, WASHBUFFER NO
45688574 CONTAINER, LIQUID WASTE NO
200412 DEEP WELL PLATE (50X) NO
200429 DEEP WELL STRIPS (250X) NO
45688576 KEY, DOOR NO
45688577 MICROPLATE HOLDER NO
45688578 RACK, CONTROL NO
200413 REAGENT BOTTLE WITH CAP (24X) NO
7513060541 REAGENT RACK NO
200411 REAGENT TIP, 1100 µL (4 X 108) NO
200415 SAMPLE RACK, 75 MM TUBES (1X) – WITH SPACER NO
SAMPLE RACK, 100 MM TUBES (1X) – WITHOUT NO

200414
SPACER
200410 SAMPLE TIP, 300 µL (4 X 108) NO
7513060543 TIP FUNNEL NO
11.4 OTHERS
Tool, Tension Meter (order locally)
Brecoflex Model SM4
®
12 R E PA I R & C L E A N I N G & D E C O N TA M I N AT I O N
PROCEDURE
The system is constructed from materials that are resistant to chemical attack.
Spills should be cleaned up as soon as possible. The service engineer should clean and
decontaminate the system before servicing.
IMPORTANT! Decontamination is mandatory when the system or a module is returned

to bioMérieux France, e.g. for repair.
12.1 REPAIR PROCEDURE FOR INSTRUMENT MODULES
Only the following modules can be returned for repair ; reader, drawer, barcode scanner,
incubator, pipette and washer. The IVT and OPSYS tools must be returned for
recalibration on a regular basis.
Fill out the form CFO0142 in use and send this document to bioMérieux
France, Global Supply Chain to receive the bioMérieux Return
Authorization Reference and a new module. The correct fax number can
be found on the document. The fax number can be found on the
document.
− For bioMérieux subsidiaries there is no warranty.
− For distributors there is a warranty period of 1 Year.
After receiving the CFO0142 form Logistics France will send you a “new
module” from stock. Keep in mind that the modules on stock might be
looking as “being used”. There is no difference between new manufactured
modules and repaired modules.
After receiving the Return Authorization Reference from France, fill out the
"Repair Sheet” (see next pages). Modules without such a form or without a
decontamination certificate will not be accepted !! (Do not forget to indicate
the bioMérieux Return Authorization Reference. Leave the Dynex Return
Authorization Number blank.)
Indicate the fault condition clearly on this paper.
Send the repair sheet to Global Supply Chain, bioMérieux France.
Send the defective module to the bioMérieux central warehouse.
NOTE: This procedure means that the defective module will immediately be
replaced by another module. You have not to wait until the module has
been repaired by Dynex.
®
Repair & Cleaning & Decontamination Procedure
Cleaning
IMPORTANT! Due to some local custom rules in some countries (e.g. Brazil) a module
can only be sent for repair without high export costs, if the same module
(same serial number) is returned after repair. These countries have to wait ±
2 months until the module has been repaired. For the pipette assembly it
will take even 4 months. So, these countries have to organize a larger local
stock to service the instruments.
12.2 CLEANING
Clean all external surfaces with a cloth moistened with a mild laboratory detergent. If
necessary, dilute the detergent according to the manufacturer’s instructions before using.
12.3 DECONTAMINATION
Wipe all surfaces, plate carriers, pipette spigot, module plate carriers with a cloth
moistened with a disinfectant cleaner (e.g. Mucasol or Extran) or a 70 % (by volume)
solution of Isopropyl alcohol.
The manifold of the washer needs to be decontaminated very well. The manifold can be
soaked in a bottle of disinfectant cleaner (e.g. Mucasol or Extran) for 30 minutes.
®
Repair & Cleaning & Decontamination Procedure
Decontamination
®
13 RUNNING AN A S S AY WITH A LIS
CONNECTION
13.1 PRINCIPLE
The LIS - Link software (version 1.06) based on the ASTM standard, is included with
the instrument. The software and documentation can also be downloaded from the
GCS Portal.
PC HOST
Network TCP / IP or Serial RS-232
You have to use barcode Sample ID’s scanned by the QUATTRO.

The HOST has to send a joblist to the QUATTRO after the HOST
received the query from the QUATTRO (bi-directional). Results
transfer only is not possible.
®
Running an assay with a LIS connection
13.2 RUNNING AN ASSAY WITH A LIS CONNECTION
Once the installation and the configuration are complete follow these procedures to
process test runs.
1. Create a new worklist.
NOTE: Make sure scanning and using LIS are selected during the worklist
creation.
2. Press OK. Select the last sample and Load the Sample Caddy. Then, scan
the sample ID’s.
®
3. After scanning the sample IDs, a query will be sent to the HOST. The HOST
will send the joblist.
4. After the Quattro has received the joblist, automatically the next screen
appears. Press “New Plate” and select the assay(s) to be run. Automatically,
the samples will be flagged for the correct assays.
5. Press OK to start the run. Perform required operations, loading fluids, etc…
6. Allow the test runs to go to completion.
7. Review the plate data.
®
8. Start the LIS-Link software (the password is “dynex”).
The Assay Status can be :

Pending (test not started)
Queued (test running)
Completed (test finished)
Errored (test aborted)
9. Click on the name of the assay that was run and review all results returned
by the QUATTRO software.
NOTE: Make sure Send is selected for the results to be transmitted to the
LIS host computer.
®
10. After the review is complete, transmit the results to the host computer.
Operations - Commit Operations - Send Data To LIS Host
After transmitting the results, the data is automatically deleted from the LIS
database. It’s not possible to resend the results.
Example :
H|\^&|||QUATTRO||||||||1|20070419170930
P|1||0009805
O|1|0009805||^^^HIV|R|20070419165339
R|1|^^^HIV|0.764|OD||RW||F||||20070419170930|1DXC1113
R|1|^^^HIV|React|||TH||F||||20070419170930|1DXC1113
R|1|^^^HIV|7.346|||FR||F||||20070419170930|1DXC1113
P|2||0009859
O|1|0009859||^^^HIV|R|20070419165339
R|1|^^^HIV|0.003|OD||RW||F||||20070419170930|1DXC1113
R|1|^^^HIV|-|||TH||F||||20070419170930|1DXC1113
R|1|^^^HIV|0.029|||FR||F||||20070419170930|1DXC1113
L|1|N
Reprocessed Plate Data (RW) can only be sent to LIS if the information is still into
the LIS database.
NOTE: By using the LIS database viewer, data still present in the database
can be deleted.
The QUATTRO LIS database “lims.mdb” is a MS Access database stored in the

folder C:\ Program Files \ Common Files \ Dynex Technologies \ Databases
The outgoing query and the incoming joblist is stored into the log file
“LogDebugActions.txt”.
This log file is stored in the folder C:\ Program Files \ Common Files \ Dynex
Technologies \ LIS-LINK
®
14 KNOWN PROBLEMS / RESOLUTION
In this chapter the known problems and the cause will be listed.
Problems Resolution
Incubators: temperature problem (out − Replacement of the module.

of range/ high/ low).
The temperature is increasing to − Securing the cable of the lower
50° C and more after some time. heating plate might resolve the
problem.
− Incubator Firmware will warn the
operator during the self test.
The Reader Self Test fails on the lamp − Check the lamp duty cycle. See page
margin 6-7. Clean the optics and filter
405 nm.
− If the LDP is higher than 120 after
cleaning, replace the 405 nm filter.
− This problem occurs in countries with
high humidity.
− A modification kit is also available to
avoid dust enters the optic box.
Note: In countries with high humidity,

we observed that the 405 nm
filter is blocking more and more
light after some time. This
degradation of the filter will result
in a lamp margin failure during
the self test. Ensure a 405 nm
filter is always available as spare
part.
Ghosting: Readers and Incubators − Rebooting usually corrects the

not always recognized on startup. problem. Use the C0MAP command
to check the availability of the
modules. Remove and re-install
modules which are not recognized. If
the problem appears again, replace
the module.
When starting a new worklist and − Replace the pipettor assy.
after selecting the Load Sample
Caddy button, the "Load Sample − Strange software errors (e.g run time
Caddy 1" screen does not appear. No errors are mostly related to a
error message was logged and the defective pipettor assy.
selftest passed.
®
Known problems / resolution
Washer z-movement error. − Replace the washer module.

− You have to train the operators that
it’s not allowed to move the washer
manifold by hand when the system is
powered.
The reagent tip is not released by the − A particular batch of reagent tips was
pipettor. giving problems with releasing the
tip. Due to incorrect dimensions of
the tip, the tip could not be released.
− Replace the batch of reagent tips.
Note: In some cases replacement of

the pipettor assy was decreasing
the number of failures. Some
pipettors were also not having
the correct spigot dimensions.
The combination of the “wrong”
pipettor and the “wrong” reagent
tip was giving the problems.
This problem is resolved now

(since end 2007). The
production of the tips has been
improved and Dynex is checking
the tips. However, it’s still
possible that you have some old
batches of reagent tips.
Carry-over problems with HBsAg − Check the system settings.

Ultra or TP.
− Software version 6.10a:
− The shake parameters and the
protocols (on correct aspirate /
dispense speed settings and correct
plate selection) have been corrected.
The airgap should be set to 63.
− If all settings are correct, replace the
pipettor assy.
HCV controls out of range. − Ensure the operator is mixing the
conjugate.
Out of the box problems (e.g. aspirate − Replace the defective parts.
pump is not working, washer is not
working, firmware can not be
updated, …).
The Pipettor might crash when the − Select the correct option. Repeat the
WFV failed and the operator selects reading when the pipettor is busy
the wrong error recovery option. with another step.
®
Known problems / resolution
No sample liquid is dispensed and no − This can be avoided by using ESP.

error is reported by the system.
Note: A false liquid detection might

occur when the tip inlet is partly
blocked.
Advice : Do not re-use tips. It can result in
a partly blocked inlet of the tip.
No reagent or control liquid is ESP is not working for reagents and
dispensed and no error is reported by controls.
the system.
− Ensure the operator knows about the
correct liquid handling.
− Air bubbles on the surface of the
liquid can result in wrong liquid
detections.
− See the AS Training documentation.
− To investigate this problem, send the
log files to GCS for further
investigation.
®
15 A D D E N D U M : P I P E T T I N G Q U A L I F I C AT I O N
NOTE: Pipetting qualification includes verification, and possibly calibration

only if verification is not OK. Exceptionally during installation, the
sample pipetting qualification needs systematical calibration.
WARNING! Text files issued from daVINCI QUATTRO software are using dots
as decimal delimiter. After using Assistant conversion for Text
data, when pasting values into Excel sheets, you may need to
convert this decimal delimiter into a comma, depending on EXCEL
settings.
0.620
For 96 cells, search « . » and replace by « , »
1. Sample pipette qualification

2 cases:
• Preventive or curative maintenance: FSE will run a Sample Verification.
(D4_UK_SampVer_2_QP_01.asy ). If Sample Verification is not conform,
FSE must adjust (Sample) single shot Calibration Values.
• Quattro installation: FSE will run directly a Calibration protocol
(D4_UK_Calib_2_QP_02.asy)
®
Addendum: Pipetting qualification
S AM P L E s i n g l e s h o t a d j u s t m e n t
Preventive or curative maintenance Quattro installation
Volume SampVer is not conform

verification in Dprog Excel file / ‘Results’ sheet
In Quattro software, set single shot see SM §2.5.4.2 Default parameters setting
Calibration Values to default values
Computation
of
new factors
values Run a ‘D4_UK_Calib_2_QP_02.asy’ protocol
Paste data results from generated .txt file

into Dprog Excel file / ‘Calib’ sheet see SM §2.5.4.3 Calibration run
If at least one Accuracy

No
cell is in yellow in 3.1
(Sample) Pipette
calibration (CALIB)
Yes
Set Calibration Volumes to Calibration

New Calibration values Values in
computed in Dprog /“Results” Quattro
sheet software
Run a ‘D4_UK_SampVer_2_QP_01.asy’ protocol
Final Volume Paste data from generated .txt file into Dprog / see SM §2.5.6.1 sample
verification ‘SampVer’ sheet verification (single shot)
Check in Dprog / ‘Results’ sheet that SampVer

results are conform (all green)
®
2. Reagent pipette qualification
In all case, firstly, Reagent Verification must be performed. If results are OK, the
Reagent pipette qualification is finished. If not, follow the procedure below.
REAGENT multishot adjustment
ReagVer is not conform

Volume
in Dprog Excel file / ‘Results’ sheet
verification
see SM §2.5.4.2 Default parameters setting

In Quattro software, set all multishot
Calibration Values to 25µL (Normalised
factors =1)
Computation
of
new factors Run worklist "multi25_2plates.wlt"
values
see below : “how to run worklist
Paste data results from the 2 generated.txt "multi25_2plates.wlt" ?”
files into Dprog / ‘multi25_1(optional)’ and
‘multi25_2(optional)’ sheets
Set Calibration
Volumes to New
Calibration values
Setting of
computed in
Calibration
‘Pipette reagent cal
factors
(optional)’ sheet
Calibration
values
(Quattro
software)
Run another
D4_UK_ReagVer_2_QP_03.asy protocol
see SM §2.5.6.2 Reagent

Final Volume Paste data from generated.txt file on Dprog / verification (multiple shot)
verification ‘second ReagentVer’ sheet
Check inDprog / ‘Results’ sheet that second

ReagVer results are conform (all green)
®
Example of ‘Results’ sheet :
How to run a worklist "multi25_2plates.wlt" ? :

• Click on File, new, worklist
• Then, click on “OK” in sample batch selection, with Add assays using a
new batch of samples selected
• Click on “Load template” button :
Load Template
• Double click on “multi25_2plates.wlt”
®
On following screen, 2 multi25 plates are declared :
• Click with the right mouse button on the first position of the test column of
<New plate 1>
• Select # of Samples From Here
• Select 96 samples
• Click with the right mouse button on the first position of the test column of
<New plate 2>
• Select 96 samples
• When the Load Sample Rack screen appears, the green ‘V’ button.
• Load a Break-4 empty plate on the correct drawer position, enter a plate
identifier in the entry box and then press the green ‘V’ button.
• Load a second Break-4 empty plate on the correct drawer position, enter a
plate identifier in the entry box and then press the green ‘V’ button.
• After each of the 2 plates is finished, the results file can be found in two
.txt files in folder C:\ProgramFiles\bioMérieux\DA VINCI QUATTRO
Program \Text.
• The two files that were generated must be imported in Excel as follows:
• Start the Excel program, and for each of the two files :
• Select File Open (*.* all files).
• Use the Browse function to select the file to be imported.
®
• Select Delimited -> Next.

• Copy Cells A1 until M40.
• Paste these cells in the corresponding sheet of the Dprog Excel sheet
starting at cell A1 : ‘multi25_1 (optional)’ sheet and ‘multi25_2
(optional)’ sheet.
®
16 A P P E N D I X : I N S TA L L AT I O N O F T H E I V T
L O G G E R S O F T WA R E
• Unzip the file IVT Logger Software.zip. This file can be downloaded from
the GCS Portal.
• Select the Setup.exe file to start the software installation.
• Click Next.
• Click Next.
• Click Next.
®
Appendix: Installation of the IVT Logger Software
The Logger software will be installed on the C drive under the folder OMEGA.
• Click Next.
• Click Finish.
®
Appendix: Installation of the IVT Logger Software
When starting the Logger software for the first time, the following message may
appear.
• Press OK.
This message will no longer appear.
®

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VERSION 6.

This manual is intended for bioMérieux staff only.

bioMérieux S.A. 69280 Marcy l'Etoile / France

The list of revisions below summarizes replacements or additional pages in your

A (6.02) 2006/05 Creation – BTL 0328222 All

B (6.08) 2008/10 Update All

C (6.10a) 2010/04 Update related to software version 6.10a All

2.6 Burn-in ................................................................................................................................................2-78

4 Start Up & SelF Test

6 Hardware & Troubleshooting & Disassembling

7 Calibration & Verification

7.3.2 Verification of the sample barcode scanner..........................................................................7-20

8 ESP (Electronic Signature Pipetting)

11 Spare parts list

12 Repair & Cleaning & Decontamination Procedure

13 Running an assay with a LIS connection

14 Known problems / resolution

15 Addendum: Pipetting qualification

16 Appendix: Installation of the IVT Logger Software

NOTE: Screenshots, figures, and messages are given for information

DAVINCI QUATTRO Computer +

Control Bottles (344 pcs) Caps, Control bottles (2000 pcs)

Reagent Bottles (24 pcs) Reagent tips (4 x 108 pcs)

Sample tips (4 x 108 pcs) Strip plates (100 pcs)

Diagnotic Kit Application Notes User CD

1.1 DAVINCI QUATTRO (ART. CODE 200408)

The complete list is available on the GCS portal.

RS 232 cable, PC - QUATTRO

Cleaning Wire, dispense needles , 0.018” , 2 pcs (art. code 45688572)

Cleaning Wire, aspirate needles , 0.040” , 2 pcs (art. code 45688571)

Deepwell dilution strip Holder, 2 pcs (art. Code 7513093643)

Deepwell dilution plate holder, 2 pcs (art. Code 7513060542)

Control Rack (art. code 45688578)

Reagent rack (art. code 7513060541)

Tip funnel (art. Code 7513060543)

Caddy, sample racks (art. code 45688575)

Plastic Tips, sample, set of 4 x 108 (art. code 200410)

Plastic Tips, reagent, set of 4 x 108 (art. code 200411)

Reagent bottle with cap, set of 24 (art. code 200413)

Purge trays, 5 pcs (art. code 45688570)

Plate carrier, 4 pcs (art. code 45688577)

Tray, washer module (dynex code 13001790)

Head assembly, 8 way (art. code 45688526)

Power Cord, 220 V , European (dynex code 575100600)

Hex Driver, 4 mm (dynex code 42000360)

Key, door (art. code 45688576)

1.2 SAMPLE RACKS FOR LONG OR SHORT TUBES

Sample Rack, 75 mm tubes, 14 positions , 7 pcs (art. code 200415)

Rack with spacer

Sample Rack, 100 mm tubes, 14 positions , 7 pcs (art. code

Rack without spacer

1.3 CONTROL BOTTLES

Control bottle, set of 344 (art. code 272240)

1.4 CAPS FOR CONTROL BOTTLES

Cap, Control bottle, set of 2000 (art. code 272241)

1.5 REAGENT BOTTLES

Reagent bottle with cap, set of 24 (art. code 200413)

1.6 DEEP WELL PLATES

Deep well plate, 10 pcs (art. code 200412)

1.7 DEEP WEEL DILUTION STRIPS

• Select File Open (. all files)