Scribd Logo
Upload

Welcome to Scribd!

  • 11 views

    MICROS

    Uploaded by

    Kristine Angie Ramos

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd

    MICROS

    Uploaded by

    Kristine Angie Ramos
    11 views4 pages

    Original Title

    MICROSCOPY

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Download as pdf
    11 views4 pages

    MICROS

    Uploaded by

    Kristine Angie Ramos

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Download as pdf
    of 4
    NAME: RATING: GROUP NO.: DATE: Exercise No. 3 MICROSCOPIC LXAMINATION OF MICROORGANISMS IN THE LIVING STATE Microorganisms are a diverse group that manifest varied morphological features. Their characteristics can be distinguished through their shape, staining properties, colony growths, biochemical reactions, and motility, among others Motility is associated with the organs ‘for locomotion called flagella. Motile bacteria can be observed when living organisms are examined under the microscope using the wet mount method or the Hanging drop technique. ‘The wet mount method involves placing a drop of the fluid with the organisms on a glass slide and covering it with a slip. In the hanging drop technique, a cover slip with the specimen is inverted over a slide with a concavity at the center. Both preparations are then examined under the microscope. True motility should be differentiated from the Brownian movement. True motility is the independent movement of microorganisms from one place to another whereas Brownian movement is produced by currents in liquids that cause the organisms to vibrate and move. IL. OBJECTIVES At the end of the exercise, the students shall have: 1. examined motile organisms from pond water or hay infusions; 2. identified some motile organisms from the ‘samples; and 3. differentiated true motility from Brownian movement Tl. MATERIALS Water samples from pond/hay infusions Glass slides Hanging drop slides/depression slides Cover slips Petroleum jelly Applicator stick Dropper : Incculating loop Alcohol lamp PROCEDURES Wet Mount Preparation 1. On a clean glass slide, place a drop of the sample at the center. 2. Place a cover slip and examine under the LPO. 3. Observe the microorganisms and note their movement. Hanging Drop Technique 1. Place a thin ring of petroleum jelly around the edge of the concavity of a hanging drop slide: 2. Flame-sterilize an inoculating loop and use it to transfer two loopfuls of the water sample at the center of a clean cover slip. 3, Invert the hanging drop slide so that the petroleum-ringed concavity is over the drop of water on the-cover slip. Press the cover slip lightly to seal the sample. 4. Reinvert quickly so that the water sample hangs suspended from the cover slip in the concavity of the slide. 5. Do not allow the sample to drop nor touch the bottom of the concavity. If the water drops, repeat the procedure. 6. Examine under the LPO’and observe the motile organisms, which usually move a considerable distance. IV. ILLUSTRATIONS Organisms observed in: Wet Mount Preparation Hanging Drop Method V. RESULTS AND DISCUSSION ae Se an 1. In the examination of microorganisms in their living state, which is the better method, the wet mourit or the hanging drop? Why? 2. What are the different motile organisms observed in the sample? 3. Differentiate true motility from Brownian movement. Aside from flagella, what other structures enable mic —>- roorganisms to move? 5. What are the basic parts of a flagellum? eee the four arrangements of flagella in bacteria. VI. CONCLUSIONS

    You might also like