LAS EZ User Manual
LAS EZ User Manual
LAS EZ User Manual
LAS EZ
3.4.0
User Manual
LAS EZ User Manual
All reasonable steps have been taken to ensure that this publication is correct and complete, but should
any user be in doubt about any detail, clarification may be sought from Leica Microsystems, or their
accredited representative. The information in this document is subject to change without notice and
should not be construed as a commitment by Leica Microsystems. Leica Microsystems accepts no
responsibility for any errors that may appear in this document.
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Table of Contents
5
LAS EZ User Manual
Feedback
For help with any problems refer, firstly, to the Please describe the exact configuration you are
TROUBLESHOOTING section or to the section on working with and your software version,
HINTS AND TIPS. including:
§ Software Version number.
Also check the latest FAQ pages at:
www.leica-microsystems.com/education § Computer description (brand name,
operating system, RAM, language, etc).
If you still encounter problems, report any issues
or feedback concerning this software to: § Microscope or camera description.
ez.support@leica-microsystems.com § Description of the exact steps that produce
the error.
§ Screenshot of any error message.
6
Installation
OVERVIEW
§ Check your computer for a High-Speed USB / USB 2.0
port, or available space to add a High-Speed USB
card.
§ If necessary, add a USB card to your computer (PCI
card in a Desktop or PCMCIA card in a laptop).
§ If you already have a Leica microscope or camera
connected to your computer, you will be asked to
disconnect it before software installation.
§ Otherwise make sure the microscope or camera is
not connected.•
§ Install the LAS EZ software.
§ Connect or reconnect your microscope or camera to
your computer with the appropriate USB cables
provided.’
§ Windows will detect the microscope (or camera) and
install the drivers for it.
§ After the driver has been successfully installed, the
microscope or camera should be ready to use.
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LAS EZ User Manual
Before Installing
LAS EZ DVD
8
Software Installation
Installing LAS EZ
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LAS EZ User Manual
10
Hardware Installation
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LAS EZ User Manual
12
Starting LAS EZ
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LAS EZ User Manual
Overview
§ 1: MENU BAR.
§ 2: WORKFLOWS.
§ 3: CONTROL PANELS.
§ 4: VIEWER.
§ 5: THE GALLERY.
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Overview
Menu Bar
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LAS EZ User Manual
Workflows
16
Overview
Control Panels
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LAS EZ User Manual
§ The right-side and largest part of the § In the BROWSE and PROCESS views, the captured
Desktop shows the IMAGE VIEWER and image is displayed in the upper part and thumbnail
GALLERY. images in the GALLERY appear along the lower part.
18
Menu Bar
Menu Bar
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LAS EZ User Manual
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Menu Bar
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LAS EZ User Manual
Output Options
§ SHUTDOWN
(EZ4 D, ICC50 & HD Cameras).
§ MAGNIFICATION
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Output Options
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LAS EZ User Manual
Save Images
24
Output Options
After Capture
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LAS EZ User Manual
Choose from:
§ FACTORY SETTINGS: Resets
the camera to the default
settings.
26
Output Options
Magnification
These settings determine how the captured image At least one magnification must be entered for
is calibrated in LAS EZ. You can only edit them in a calibration to be possible, but any number up
the ACQUIRE workflow. to a maximum of 12 magnifications is allowed.
LAS EZ automatically creates a list of default CALIBRATION UNITS: Select the type of calibration
calibration (magnification) settings for that camera. units used at the time of capture from:
You can edit these default settings in Preferences > § Nanometers (nm).
Magnification. § Microns (µm).
§ Millimeters (mm).
§ Centimeters (cm).
There are two ways to edit the default § Meters (m).
magnification settings for a camera: § Inches (in).
§ Mils (mil).
Measured settings
The default units are millimeters.
Calculated settings
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LAS EZ User Manual
Measured Settings
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Output Options
Calculated Settings
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LAS EZ User Manual
§ CAMERA TOOLBOX:
Tools for image adjustment.
§ EXPOSURE ADJUST:
Manual controls for
optimising the image.
§ WHITE BALANCE:
Controls for optimising the
image White Balance.
§ INPUT OPTIONS:
Microscope settings.
§ CALIBRATION SETTINGS:
Calibration settings.
§ PROCESSING:
Additional processing
applied to the live image.
§ ANNOTATION:
Draw lines on the live image
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Controlling the Live Image
Camera Toolbox
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LAS EZ User Manual
Adjusting Brightness
Continued >>>
32
Controlling the Live Image
Exposure Adjust
§ If AUTO BRIGHTNESS is
enabled, the panel will show
Brightness instead of
Exposure.
§ In this mode both Exposure
and Gain are automatically
adjusted to maintain the
specified Brightness level.
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LAS EZ User Manual
White Balance
It also sets:
Auto Brightness = 75%,
Gamma = 1 and...
Saturation = 100.
34
Controlling the Live Image
It also sets:
Auto Brightness = 75%,
Gamma = 1 and...
Saturation = 100.
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LAS EZ User Manual
Input Options
CAMERA: You can select which camera is active when more than
one camera is connected.
Only one camera can be active at a time.
LAST USED: These are the settings that were in use when LAS
EZ was last used.
You can set the LIVE FORMAT independently from the CAPTURED
FORMAT.
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Controlling the Live Image
2: Click OK
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LAS EZ User Manual
2: Click OK.
38
Controlling the Live Image
SHARPENING:
Select a level (Low, Medium, Hard,
Robust or Off) to apply sharpening to
the live and captured images.
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LAS EZ User Manual
Annotation
Check Show.
40
Controlling the Live Image
Drawing a Line
Check Show.
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LAS EZ User Manual
Acquiring an Image
42
Controlling the Live Image
Recording a Movie
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LAS EZ User Manual
Image Browser
§ FILE INFORMATION.
§ DIRECTORY BROWSER.
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Viewing Captured Images and Movies
File Information
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LAS EZ User Manual
Directory Browser
46
Viewing Captured Images and Movies
Folder Options
RENAME a folder.
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LAS EZ User Manual
Image Viewer
48
Viewing Captured Images and Movies
The TOOL BAR at the side of the image displays the main tools that can be used from the
VIEWER.
§ Paste image.
§ Pan Window.
§ Zoom In.
§ Zoom Out.
§ Click this to scale the image to fit in the available display area.
§ Show the image in its original size. It may be larger than the viewer window.
Note: For movie files, these options are only available when the first frame of the movie is
displayed in the IMAGE VIEWER.
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LAS EZ User Manual
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Viewing Captured Images and Movies
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LAS EZ User Manual
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Viewing Captured Images and Movies
Playing a Movie
Elapsed time.
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LAS EZ User Manual
Image Gallery
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Viewing Captured Images and Movies
Importing Images
Click OK.
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LAS EZ User Manual
Exporting Images
Click OK.
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Processing Captured Images
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LAS EZ User Manual
Annotation
§ INFORMATION.
§ SCALE BAR,
§ LINE,
§ FONTS,
§ ACTIONS,
§ CALIBRATION.
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Processing Captured Images
Information
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LAS EZ User Manual
Scale Bar
If no Calibration is selected
the Units show as
"pp" (pixels).
Select the END STYLE of the
scale bar:
§ None,
§ Short,
§ Medium or
§ Long.
Alternatively choose a defined
length with USER LENGTH and
set the value.
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Processing Captured Images
Line
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LAS EZ User Manual
Edit Line
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Processing Captured Images
Fonts
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LAS EZ User Manual
Actions
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Processing Captured Images
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LAS EZ User Manual
Enhancement
GAMMA.
§ COLOUR.
§ ORIENTATION.
§ CROP AND...
§ CONFIRM CHANGES.
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Processing Captured Images
Brightness
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LAS EZ User Manual
Color
68
Processing Captured Images
Orientation
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LAS EZ User Manual
Crop
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Processing Captured Images
Confirm
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LAS EZ User Manual
Troubleshooting
THE LIVE IMAGE WINDOW IS COMPLETELY GRAY: THE LIVE IMAGE IS TOO DARK:
§ Check that the USB connection from the § Exposure or Gain is too low - increase Exposure
microscope to the computer is connected or Gain or both.
securely. § Gamma is set too high - decrease Gamma.
§ Check that the microscope power cable is § Auto Brightness is selected but the brightness
connected securely and the power is on at the level is too low - increase the Brightness level.
mains and at the microscope. § Check the microscope and lighting settings.
§ Check that the microscope camera drivers (still
and video) have been installed correctly and THE LIVE IMAGE IS NOT IN FOCUS:
that they are visible in the computer Hardware § Check the microscope settings and follow the
Device Manager. instructions in the microscope operating
manual.
THE LIVE IMAGE WINDOW IS COMPLETELY BLACK: § Compare the focus through the eye-piece and
§ Check the light conditions and the microscope compare with the image within LAS. It is
light settings. Ensure that light reaches the possible that they are not identical. As a
camera. temporary work-around, focus using the live
§ The incident lights are faulty or covered by an image within LAS. Contact ez.support.
obstacle. § Check the Zoom dial on the microscope. The
§ Incident light setting has been selected on the focus is automatically adjusted when zooming
microscope or in LAS and transmitted is in or out. It is possible this may be defective.
required. Contact ez.support.
§ The transmitted light box on the microscope is
faulty or has been covered by opaque POOR IMAGE QUALITY:
materials. § Check microscope light settings.
§ Transmitted light setting has been selected on § Check Exposure and Gain settings to avoid
the microscope or in LAS and incident is under- or over-exposure.
required. § Make sure the eyepiece fits snugly around the
§ Exposure or gain is too low - increase Exposure eye so no stray light enters the eyepiece. Cover
or Gain or both. the eyepiece to external light if it is not being
§ Gamma is set too high - decrease Gamma. used.
§ Auto Brightness is selected but the brightness § Check the Gain setting. An Increase in Gain will
level is too low - increase the Brightness level. increase the electronic noise of the microscope
camera. If you reduce Gain you should increase
the Exposure to compensate.
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Hints & Tips
73
-D-
Index
Date 59
Delete 36, 54
-1- Delete a Line 41
Delete Current 36, 39
1:1 48 Description 59
-A- Desktop
Directory
14
46
Discard 66
Actions 64 Distance Line 61
After capture 25 Do Nothing 25
Alt+F4 19
Annotate 58 -E-
Annotation 40, 58
Arrow 61 EC3 22, 31, 34, 37
Auto Brighness 31 Edit 61, 62
Auto Exposure 31 email 48
Auto Save 23 Enhancement 66
Auto White Balance 34, 35 Export 56
AWB 34, 35 Exposure 32
-B- EZ4 D
EZ4 HD
22, 31, 35, 38
34, 38
background 63 -F-
Bar 14
Bitdepth 24 F1 19
Bit-depth 24 F3 19
Brightness 66 F5 19
Browse 44, 46 Faulty 72
-C- Feedback
File 45
6
file format 24
Calculated 29 File information 45
Calibrate 32, 65 file type 24
Calibration 24, 27, 28, 29, 37, 38, 65 Fit 48
Calibration Units 24 Fit to Window 48
Camera 19 Flip 39, 66
Captured Format 36 Folder 23, 46
Centimeters (cm) 27 Font 63
Clear 64 Format 36
Close 19 Frame Rate 73
color 61 Freehand 61
Computer 6 Freehand Line 41
Configuration 36 Full Screen 19
Contrast 66
Control Panel 14, 17 -G-
Copy 48, 54
Crop 66 Gain 32
Customer support 6 Gallery 54
74
Gamma 32, 66 Menu 14, 15
Grayscale 66 Menu Bar 19
Merge 64
-H- Meters (m) 27
Microns (µm) 27
Help 19 Millimeters (mm) 27
Hide 54, 64 Mils (mil) 27
Hint 73 Mono 66
Horizontal 66
Hue 66 -N-
-I- Name 54
Nanometers (nm) 27
IC80 HD 22, 31, 34, 37 Navigate 48
ICC50 22, 31, 34, 37
Illumination 34, 35 -O-
Image Browser 44
Image Name 59 Open 54
Image Output settings 22 Open Image using 25
Image Viewer 14, 18, 48 Open in 25
Import 54, 55 Options 57
In this folder 23 Orientation 66
In this format 24 Output Options 19, 22
Inches (in) 27 overview 14
Information 45
Inicdent 31 -P-
Input options 36
Invert 66 Pan 48
Paste 48
-L- Preferences 28, 29
Print 48
label 61 Process 66
LAN 56 Processing 57
LAS 5
LAS EZ 5 -Q-
Last Used 36
LED 34, 35 Quality 72
Line 61
Line Color 40
-R-
Line Edit 62
Line Only 61 Rename 54
Line Thickness 40 Replace 66
Live Format 36 Reset 32, 34, 35
Load 48 Restore Default 36
-M- -S-
Magnification 27, 28, 29, 37, 38 Saturation 32, 66
Magnifier 48 Save 23, 36, 48
Manual 31, 32 Save Current 36
Measured 28 Save Images 24
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Scale 60
Scale Bar 60
Send To 48
Settings 22
Shading 39
Sharpening 32, 39
Shutdown 26
Standalone 26
Switch Camera 36
System Requirements 6
-T-
text 63
Thumbnail Sizes 54
Time 59
Tip 73
Transmitted 31
Transparent 63
Troubleshoot 72
-U-
Units 24, 27
User Length 60
-V-
Version 19
Vertical 66
-W-
White Balance 34, 35
With this bitdepth 24
With this name 23
Workflow 14, 16
-X-
Xml 56
-Z-
zero padding 24
Zoom 48
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