Part 1

Standard
concentration
(nmol/L) Absorbance
25 0.059
50 0.096
100 0.302
150 0.604
200 0.805
250 1.058

absorbance of
the unknown
sample (X) 0.5
Concentration of
Unknown sample
(X) 131.9130435 nmol/L

Part 2
Standard
concentration
(nmol/L) Absorbance
25 0.82
50 0.76
100 0.685
200 0.556
250 0.422
300 0.3
400 0.183

absorbance of
the unknown
sample (Y) 0.45
Concentration of
Unknown sample
(Y) 240.7647059 nmol/L
 Standard conc. (nmol/L) vs. Absorbance
1.2 1.058

1
0.805
Absorbance

0.8 y = 0.0046x - 0.1068

0.604
0.6
Absorbance
0.4 0.302
Linear (Absorbance)
0.2 0.0509.096

0
0 100 200 300

Standard concentration (nmol/L)

Standard conc. (nmol/L) vs. Absorbance

0.9 0.82
0.76
0.8
0.685
0.7
Absorbance

0.556
0.6
0.422 y = -0.0017x + 0.8593
0.5
0.4 0 .3 Absorbance
0.3
0.183 Linear (Absorbance)
0.2
0.1
0
0 100 200 300 400 500

Standard concentration (nmol/L)

Part 3
1. Incubate with an antibody-enzyme complex that binds primary antibody. (3)
2. Coat the surface with antigen and block unoccupied sites with nonspecific
protein. (1)
3. Add substrate, the formation of the colored product indicates the presence
of a specific antigen. (4)
4. Incubate with primary antibody against a specific antigen.(2)