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Standard Conc. (Nmol/l) vs. Absorbance

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Nour Sa'eed
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44 views2 pages

Standard Conc. (Nmol/l) vs. Absorbance

Uploaded by

Nour Sa'eed
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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Part 1

Standard
concentration
(nmol/L) Absorbance
25 0.059
50 0.096
100 0.302
150 0.604
200 0.805
250 1.058

absorbance of
the unknown
sample (X) 0.5

Concentration of
Unknown sample
(X) 131.9130434783 nmol/L

Part 2
Standard
concentration
(nmol/L) Absorbance
25 0.82
50 0.76
100 0.685
200 0.556
250 0.422
300 0.3
400 0.183

absorbance of
the unknown
sample (Y) 0.45

Concentration of
Unknown sample
(Y) 240.7647058824 nmol/L

Standard conc. (nmol/L) vs. Absorbance


1.2 1.058
1 f(x) = 0.0045998904109589 x − 0.106819178082192
0.805
ance

0.8
0.604
0.6 Absorbance
Standard conc. (nmol/L) vs. Absorbance
1.2 1.058
1 f(x) = 0.0045998904109589 x − 0.106819178082192
Absorbance 0.805
0.8
0.604
0.6 Absorbance
Linear (Absorbance)
0.4 0.302

0.2 0.059
0.096

0
0 50 100 150 200 250 300

Standard concentration (nmol/L)

Standard conc. (nmol/L) vs. Absorbance


0.9 0.82
0.8 0.76
f(x) = − 0.00172771384136858 x + 0.859317262830482
0.685
0.7
0.556
Absorbance

0.6
0.5 0.422
Absorbance
0.4 Linear (Absorbance)
0.3
0.3
0.183
0.2
0.1
0
0 50 100 150 200 250 300 350 400 450

Standard concentration (nmol/L)

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