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    CMB Lab 1... M

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    Den Angelica Dungo
    1. The document describes the anatomy and functions of parts of a compound microscope. It lists and defines parts like the oculars, diopter adjustment ring, objective lenses, mechanical stage, condenser system, illuminator, and others. 2. It provides examples of calculating total magnification by multiplying the ocular magnification by the objective magnification. For red blood cells viewed under 40x with a 12.5x ocular, total magnification is 500x. For Euglena viewed under 100x oil immersion with a 5x ocular, total magnification is 500x. 3. The document is a laboratory activity sheet that instructs students to draw and label a microscope, identify microscope

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    CMB Lab 1... M

    Uploaded by

    Den Angelica Dungo
    68 views4 pages
    1. The document describes the anatomy and functions of parts of a compound microscope. It lists and defines parts like the oculars, diopter adjustment ring, objective lenses, mechanical stage, condenser system, illuminator, and others. 2. It provides examples of calculating total magnification by multiplying the ocular magnification by the objective magnification. For red blood cells viewed under 40x with a 12.5x ocular, total magnification is 500x. For Euglena viewed under 100x oil immersion with a 5x ocular, total magnification is 500x. 3. The document is a laboratory activity sheet that instructs students to draw and label a microscope, identify microscope

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    CMB Lab 1...m

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    1. The document describes the anatomy and functions of parts of a compound microscope. It lists and defines parts like the oculars, diopter adjustment ring, objective lenses, mechanical stage, condenser system, illuminator, and others. 2. It provides examples of calculating total magnification by multiplying the ocular magnification by the objective magnification. For red blood cells viewed under 40x with a 12.5x ocular, total magnification is 500x. For Euglena viewed under 100x oil immersion with a 5x ocular, total magnification is 500x. 3. The document is a laboratory activity sheet that instructs students to draw and label a microscope, identify microscope

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    68 views4 pages

    CMB Lab 1... M

    Uploaded by

    Den Angelica Dungo
    1. The document describes the anatomy and functions of parts of a compound microscope. It lists and defines parts like the oculars, diopter adjustment ring, objective lenses, mechanical stage, condenser system, illuminator, and others. 2. It provides examples of calculating total magnification by multiplying the ocular magnification by the objective magnification. For red blood cells viewed under 40x with a 12.5x ocular, total magnification is 500x. For Euglena viewed under 100x oil immersion with a 5x ocular, total magnification is 500x. 3. The document is a laboratory activity sheet that instructs students to draw and label a microscope, identify microscope

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Pangasinan State University

    BAYAMBANG CAMPUS
    Zone VI, Bayambang, Pangasinan

    CELL and MOLECULA BIOLOGY


    Sci 121 Laboratory
    ------------------------------------------------------------------------------------------------------------------------------------------
    Name: Den Angelica Dungo Score:
    Year and Section: BSE SCIENCE II-2 Date:
    Laboratory Activity No. 1
    THE MICROSCOPE

    I. Draw and label a compound microscope.


    Pangasinan State University
    BAYAMBANG CAMPUS
    Zone VI, Bayambang, Pangasinan

    CELL and MOLECULA BIOLOGY


    Sci 121 Laboratory
    ------------------------------------------------------------------------------------------------------------------------------------------
    II. Write the functions of the parts of a compound microscope.
    Anatomy of a microscope
    Oculars
    The oculars have lenses that magnify images 10 times (10x). Inside the right ocular is a pointer
    which can be moved by rotating the ocular. The right ocular is loose, while the left ocular is
    secured in place. This is for Köhler illumination.  The oculars sit in the ocular tubes.

    Diopter Adjustment Ring


    This ring is used to accommodate the fact that both of your eyes may not be focused the same.
    Instructions on how to use this part are given below. This ring is found on both ocular tubes

    Ocular Tube
    The ocular tubes hold the oculars, and can be adjusted for interpupillary distance, the distance
    between your eyes.

    Head
    This part of the microscope contains a delicate prism system which helps to send an image to
    the oculars and your eyes.

    Body
    This part of the microscope houses the revolving nosepiece or Turret and objective lenses.

    Revolving Nosepiece or Turret


    This part of the microscope contains four objectives at various magnifications.

    Objective Lenses
    Your microscope is equipped with four objective lenses with magnifications of 4x, 10x, 40x, and
    100x. The 100x objective is an oil immersion lens. The longer the objective, the more
    magnification it has.

    Arm
    This part of the microscope essentially holds all of the other parts, and is used in the transport
    of the microscope.

    Course Focus Knob


    This knob located on both sides of the microscope allows you to focus your image in the
    microscope.

    Fine Focus Knob


    Pangasinan State University
    BAYAMBANG CAMPUS
    Zone VI, Bayambang, Pangasinan

    CELL and MOLECULA BIOLOGY


    Sci 121 Laboratory
    ------------------------------------------------------------------------------------------------------------------------------------------
    This knob “fine tunes” the focus of your specimen.

    Base
    This part of the microscope holds everything in place, and is used in the transport of the
    microscope.

    Mechanical Stage
    This is where the specimen is placed for observation. The slide holder has a clamp which can
    swing out to hold the slide. The lever which opens the clamp is on the left side of the
    microscope. With a slide in place, it can be moved in the X and Y directions using the stage
    control knobs.

    X Stage Control Knob


    This knob will move a slide in the X-axis (horizontally) on the mechanical stage.

    Y Stage Control Knob


    This knob will move a slide in the Y-axis (vertically) on the mechanical stage.

    Condenser System
    This is a system of lenses which helps to focus light directly on the specimen that is mounted on
    a slide.

    Diaphragm Lever
    This lever is used to control the diameter of the  diaphragm.

    Condenser Focus Knob


    This knob is used to focus light properly on the mounted specimen.

    Field Iris Diaphragm


    This system is used to vary the diameter of the field iris diaphragm, limiting the amount of light
    passing through the condenser system and the specimen.

    Brightness Control Knob


    This knob controls the brightness of the light.

    Illuminator
    Housing a 6 V 20 W halogen bulb within the base of the microscope, this system provides light
    for specimen illumination.
    Pangasinan State University
    BAYAMBANG CAMPUS
    Zone VI, Bayambang, Pangasinan

    CELL and MOLECULA BIOLOGY


    Sci 121 Laboratory
    ------------------------------------------------------------------------------------------------------------------------------------------
    Aperture: The hole in the middle of the stage that allows light from the illuminator to reach the
    specimen.

    On/off switch: This switch on the base of the microscope turns the illuminator off and on.
    Illumination: The light source for a microscope. Older microscopes used mirrors to reflect light
    from an external source up through the bottom of the stage; however, most microscopes now
    use a low-voltage bulb.

    III. Compute for the total magnification. Show your complete solution.
    1. What is the total magnification of red blood cells sample viewed under HPO using an
    ocular with a magnification of 12.5x?

    Total Magnification = Ocular lens power x Objective Lens Power


    =12.5x X 40x
    =500x

    2. Compute for the total magnification of a Eugkena viewed using an ocular with a
    magnification of 5x and under OIO.

    Total Magnification = Ocular lens power x Objective Lens Power


    5x X 100x
    = 500x

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