S P E C T R O P H O T O M E T E R

SP-UV1000

USER MANUAL
 Contents
Safety ............................................................. 1

Package Contents ................................................... 1

Unpacking .......................................................... 1

Installation ....................................................... 2

1. Environment Required.................................................................................................. 2

2. Install Spectrophotometer...................................................................................... 2

Overview ........................................................... 2

Symbols ............................................................ 3

Main Specifications ................................................ 3

Description of Appearance and Keys ................................. 4

1. Appearance ......................................................................................................................... 4

2. Keypad ................................................................................................................................... 5

3. Description of Keys .................................................................................................... 5

Functions .......................................................... 6

Getting Started .................................................... 6

Important Guidelines ............................................... 7

General Operating .................................................. 7

Measuring .......................................................... 8

1. Photometry ......................................................................................................................... 8

2. Quantitation..................................................................................................................... 9

3. Utility................................................................................................................................. 9

Troubleshooting ................................................... 15

Repair and Maintenance ............................................ 16

1. Daily Maintain .............................................................................................................. 16

2. Spare Parts Replacement ........................................................................................ 16

I
Warranty .......................................................... 20

Equipment Disposal ................................................ 20

II
Safety
Please follow the guidelines below, and read this manual in its entirety
to ensure safe operation of the unit.

We recommends against the use of SP-UV1000 Spectrophotometer.

 Do not open the device.

 Disconnect the device from the mains supply before
carrying out maintenance work or changing the fuses.
 The inside of the device is a high-voltage area Danger!
 Do not use the device if it is damaged, especially if the
main power cable is in any way damaged or defective.
 Repairs may only be carried out by the service technicians
fromus and authorized contractual partners.
 The device must be connected to a power outlet that has
a protective ground connection.
 If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment
may be impaired.

 Do not allow any liquid to enter into the device.

 Do not operate the device in a hazardous location or
potentially explosive environment.

Package Contents
Description Quantity
Spectrophotometer 1PC
10mm Glass Cuvette 4PCS
10mm QuartzCuvette 2PCS
Power Cord 1PC
User’s Manual 1PC
Dust Cover 1PC

Unpacking
Open the package, according to carefully check the packaging packing list
items, if found inside the packaging are missing or damaged items please

1
contactus and authorized contractual partners.

Installation
1. Environment Required
To ensure the best performance, the following conditions are required:

 The best working temperature range is 16—35℃and the humidity is

45—80%.
 Keep it as far as possible away from the strong magnetic or
electrical fields or any electrical device that may generate
high-frequency fields.
 Set the unit up in an area that is free of dust, corrosive gases
and strong vibrations.
 Remove any obstructions or materials that could hinder the flow
of air under and around the instrument.
 The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.
 Use the appropriate power cord and plug into a grounded outlet.
 If the local voltage is not stable, a voltage regulator is required.
 Be away from direct sunlight.

2. Install Spectrophotometer
Placement
Place the instrument on the stable table carefully.

Install Printer (Printer is Optional Accessories)

Check to confirm instrument power switch is turned off,connectthe
printer’s data cable to the instrument’s parallel port.

Link the Power Cord

Check to confirm instrument power switch is turned off, the power cord
plug into two separate power interface and power supply socket apparatus.

Overview
SP-UV1000 Spectrophotometer is an electrical measure instrument which is
widely used in the laboratories.

 Use Frequency: Intermittence

 Excessive Voltage(Current): No
 Pollution Class: Class 1
2
Symbols
The following chart is an illustrated glossary of the symbols that are
used in this manual.

Caution, Danger!

Caution, High Voltage!

Caution, Hot!

Ground

Fuse

Recycle, this instrument will be called back by the

appointed Electrical Treatment Department or by the
original Manufacturer when wasted.

Main Specifications
 Optical System Single beam
 Wavelength Range 200—1000nm
 Wavelength Accuracy ±2nm
 Wavelength Repeatability 0.8nm
 Photometric Range -0.3—3A, 0—200%T
 Photometric Accuracy ±0.5%T
 Photometric Repeatability 0.3%T
 Spectral Bandwidth 4nm
 Stray Light 0.3%T@220nm&360nm
 Stability ±0.002A/h@500nm
 Work Mode Photometry, Quantitation
 Interface USB, Parallel(printer)
 Power Requirement AC 110/220V, 50/60Hz

3
  Dimensions 490x360x210
 Weight 14kg
 Work Environment 15—35℃,15—70% relative humidity
 StoreEnvironment -10—50℃, 15—70% relative humidity

Description of Appearance and Keys

1. Appearance
Front View

3 1

Back View

8 11

6
5 7 9 10

1 LCD Display 7 USB port

2 Keypad 8 Cover of Fan
3 Lid of Sample Room 9 Power Socket
4 Rod 10 Power Switch
5 LCD Contrast Adjust 11 Cover of Cooling Vents
6 Printer port

4
2. Keypad

3. Description of Keys

SET SET Key: Set Parameters

G O TO ʎ GOTO ʎ Key: Set Wavelength

ZE R O ZERO Key: Blank

P R IN T PRINT Key: Print measuring result

5
 Function Key:Functions according to the screen

UP, DOWN Keys: Scroll menu/data and set Y scale

,

Functions
Photometry
Display results as Abs, %T or Energy.

Quantitation
Use a Standard Sample to establish Standard Curve.

Getting Started
The following chart describes the basic operation of the instrument.

Turn On and Self-check

Switch on the power. Then the
instrument begins to self-check and 20
minutes’ warm up. Self-check includes
the following steps:Turn on lamps →
Check Sensor → Initialize AD → System
position → Get Dark Current → Warm up.
Self-test...

Warm up 20 minutes,
Any key to skip

After warm up, instrument displays Main

Interface. WL: 500.0nm 0.000A

100.0%T
Basic Quantitative
6
Important Guidelines
 Reagents and dilution buffers can cause cauterization and other damage
to health.
 Samples (nucleic acids, proteins, bacteria cultures) can be infectious
and cause serious damage to health.
 During sample preparation, measuring procedures and maintenance and
cleaning work, observe all local laboratory safety precautions (e.g.
wear protective clothing and gloves, use of disinfectant) regarding
the handling of sample material.
 Dispose of measuring solutions and cleaning and disinfectant materials
in accordance with the relevant local laboratory regulations.

General Operating
Select Application

Main interface, press the key (left) to enter into.

Set Wavelength

G O TO ʎ
Test interface, press key to set wavelength, , to modify

wavelength value, then press key (left)to go to wavelength and blank.

WL: 500.0nm
Please enter WL.:
500.0 nm
OK

Set Parameters

SET
Press enter into setup interface, , to select items or

input parameters, (left) to confirm.

Delete the test result and stored data

P R IN T
Test Interface, press the key , then press key , to select

7
“Clear Data, not Print ”, (left)to delete.

Blank

ZE R O
Put the Reference in the light path, press to do blank.

Measure Samples

Put the samples in the light path, press (left) to measure.

Print the test results

P R IN T
Test Interface, press the key , , select “Print, clear

data”, press the key (left) to print.

Store the Standard Curve

After got the Standard Curve by Marked, press , input the file

name and press (left) to save.

Load the Standard Curve

“Quantitative” interface, press , to select “Load Curve”, press

, to choose the curve you want, press (left) to open.

Measuring
1. Photometry
Step 1. StartPhotometry

MainInterface, press key (left) to choose “Basic”.

WL: 500.0nm 0.000A WL: 500.0nm 0.000A

No. WL %T Abs.
100.0%T
Basic Quantitative Test Cancel
8
Step 2. Set Wavelength

G O TO ʎ
Press to set wavelength,press , to input wavelength

value, press (left) to go to wavelength.

Step 3. Blank

ZE R O
Put the Reference in the light path and press to do blank.

Step 4. Measuresamples
Put the sample in the light path, and then the result displays on the

screen automatically, press (left) to record.

WL: 500.0nm 0.000A

No. WL %T Abs.
1 500.0 100.0 0.000
2 500.0 100.0 0.000
Test Cancel

2. Quantitation
Step 1. Start Quantitation

Main Interface, press key (left) to choose “Quantitative”.

WL: 500.0nm 0.000A Create Curve

Load Curve
100.0%T Clear Curve
Basic Quantitative OK Cancel

Step 2. Establishor call Standard Curve

Create Curve WL: 500.0nm

Load Curve Coefficient
Clear Curve Standard Curve
OK Cancel OK Cancel

9
 2 methods to establish Standard Curve:

Method 1: Establish Standard Curve byinputting coefficients

1) Starts establish.Press , to select “Coefficient” , then

press (left) to confirm.

WL: 500.0nm WL: 500.0nm

Coefficient Work WL.:
Standard Curve 500.0 nm
OK Cancel OK Cancel

2) Set wavelength. Press , to input test wavelength value,

press (left) to confirm.

3) Set coefficient Kand B. Press , toinput coefficient K,

press (left), same way set B.

WL: 500.0nm WL: 500.0nm

Work WL.: Coefficient K:
500.0 nm 00000
OK Cancel OK Cancel

Method 2: Establish Standard Curve by using Standard Samples

1) Starts establish.Press , to select “Standard Curve”, then

press (left).

WL: 500.0nm WL: 500.0nm 0.000A

Coefficient
Please insert Blank:
Standard Curve
OK Cancel OK Cancel
10
 G O TO ʎ
2) Set Wavelength.Press to enter to set wavelength, Press ,

to input wavelength value, press (left) to go the

setting value.

3) Blank. Put the Reference in the light path, press (left) to

do blank.

4) Setup number of Standard Samples.Press , toinput the

quantity of standard sample(No more than 9 standard curve.), press

(left) to confirm.

WL: 500.0nm WL: 500.0nm

Coefficient K:
00000 Number: 3

OK Cancel OK Cancel

5) Calibrate Standard Samples. Put the corresponding standard samples

in the light path as the screen indicates,press , to input

the concentration,press (left)to confirm, to finish all the

standard samples.

WL: 500.0nm WL: 500.0nm

Insert 1# Standard.
Number: 3 Input 1# Conc:00000
OK Cancel OK Cancel

Load the Stored Curve

Press , to choose “Load Curve”, press , to choose

the curve, press (left)to confirm.

11
 Create Curve C=KxA+B WL.
Load Curve C=1.000xA+0.2 500.0 <
C=0.02xA+0.32 470.0
Clear Curve
OK Cancel OK Cancel

Press (left)to enter the test mode after building or loading

standard curve.

Step 3. Enterinto MeasuringInterface

Press (left)to enter into the QuantitationMeasuringInterface.

4.000 + WL: 500.0nm 0.000A

+
+ No. WL Abs. Conc.
0.000
-0.100 0.900
r=0.996, C=2.267+1.211
Test Cancel Test Cancel

Step 4. Blank

ZE R O
Put the Reference in the light path, press to do blank.

Step 5. Measure Samples

Put the sample to be tested in the light path, press (left)to

measure. Then the test result will display in the data sheet. Repeat
this step to finish measuring all the samples.

500.0nm 0.000A
No. WL Abs Conc.
1 500.0 0.039 0.078
2 500.0 0.042 0.084
3 500.0 0.041 0.082

3. Utility
SET
Main Interface, press to go into utility setting.
12
 WL: 500.0nm 0.000A Test Mode
D2 Lamp On
100.0%T W Lamp On
Basic Quantitative OK Cancel

Test Mode

Press , to choose “Test Mode”, press (left) to enter,press

, to choose “Abs”, “%T”,“Energy”, press (left) to confirm.

Test Mode Abs

D2 Lamp On %T
W Lamp On Energy
OK Cancel OK Cancel

Turn On/Off D2 Lamp

Press , to choose “D2 Lamp”, then press (left) to enter

into.Press , o choose “On” or “Off”, press (left) to turn

on/off.

Test Mode √D2 Lamp On

D2 Lamp On On √
W Lamp On Off
OK Cancel OK Cancel

Turn On/Off W Lamp

Press , to choose “W Lamp”, then press (left) to enter

into.Press , to choose “On” or “Off”, press (left) to turn

on/off.

13
 Test Mode √W Lamp On
D2 Lamp On On √
W Lamp On Off
OK Cancel OK Cancel

Get Dark Current

Keep the light path without anything blocking, press , to choose

“Dark Current”, then press (left) to resample Dark Current.

Note: During the course, open the lid of the compartment is prohibited.

Dark Current
Reset WL
Get Dark Current!
Version
OK Cancel

Reset Wavelength

Keep the light path without anything blocking, press , to choose

“Reset WL”,then press (left) to reset wavelength.

Dark Current
Reset WL
Calibrating WL!
Version
OK Cancel

About Version

Press , to choose “Version”,press (left) to view version

information,press any key to return.

14
 Dark Current
Reset WL Software: V2.4.2
Version Hardware: V1.2
OK Cancel

Troubleshooting
Review the information in the table below to troubleshoot operating
problems.

Problem Cause Solution

Power cord connection is
Improve connectivity
Power on, no response not reliable
Fuse burning Replace fuse
Warm up is not enough Warm up more time
Sample is not Stable Improve the sample
The concentration of
Diluted sample
sample is too high
Measurement uncertainty
Power Supply Voltage Low
Improve the Power Supply
or not Stable
Lamp damage or lamp life
Replace lamp
maturity
The lid of the
Dark Current Error when
compartment is open Close the lid, restart
self-check
during self-check
Something block the Remove it, calibrate
System Calibrate Failed
Light path again
Power on, back light is
OK, but nothing display Adjust the contrast
Display Contrast problem
on the screen or display potentiometer
is not clear
Cuvettes were
Clean cuvettes
contaminated
Samples were
Improve samples
Measurements inaccurate contaminated
Worse matching of the Improve the matching of
cuvettes the cuvettes
Dark current error Resample dark current

15
Repair and Maintenance
1. Daily Maintain
Check the Compartment

After measurement, the cuvettes with sample solutions should be taken out
of the compartment in time. Or the volatilization of the solution would
make the mirror go moldy. Users must pay more attention to the corrosive
sample and liquid easy to volatilize. Any solution remains in the
compartment should be wiped off immediately.

Surface Clean

The cover of the instrument is with paint. Please use wet towel to wipe
off the drips on the surface immediately. Organic solution is forbidden
to be used to clean the cover. Please wipe off the dirt on the cover timely.

Clean the Cuvettes

After every test or after a solution change, the cuvettes should be cleaned
carefully, or the remains on the surface would cause measuring error.

2. Spare Parts Replacement

Replace the Fuse

Danger! Be sure to switch off the power and unplug the socket
before replacement!

Step 1. Tools preparation

Prepare a 3×75 Flat Blade screwdriver.

Step 2. Switch Off the power supply

Switch off the power supply, and unplug the socket.

Step 3. Take out the Fuse Seat

Take out the fuse seat by the screwdriver.

16
Step 4. Replace a new fuse
Pick out the spare fuse (3.15A/250V) and replace it to the working
position.

Step 5. Reset the fuse seat

Replace the fuse seat in the power socket.

Step 6. Switch on the power

Plug the socket and switch on the power.

Replace Lamps

Hot! Wait 20 minutes before open the lamp chamber after power
off to avoid scald!

Step 1. Tools preparation

Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.

Step 2. Power Off

Switch off the power supply and unplug the socket.

Step 3. Open the cover

Unscrew the 4 screws indicated(Each side with 2 screws)and remove the
cover.

17
Step 4. Open the cover of the light chamber
Unscrew the 2 screws on the light chamber cover and remove it.

Light
chamber

Step 5. Replace the D2 lamp

Unscrew the 2 screws on the D2 Flange (No.1), unplug the connector
in the Power Board (No. 2)and remove the D2 lamp. Draw on the cotton
glove and replace a new lamp. Fix the 2 screws and plug the connector
again.

D2
Lamp
1
2

Step 6. Replace W lamp

Pull out the defected W lamp and draw on the cotton glove. Insert the
new W lamp as deep as possible on the lamp seat. Be sure to keep the

18
 filament in the same direction as the old one face.

W
Lamp Filament

Adjust the position of the W lamp

Switch on the power(the Switch Mirror should be placed to the position

asindicates). Observe the entrance facular, and it should in the center
of the entrance hole.If the facular deviate to Left or Right, then
loosen the No.1 screws in Fig. 5-8 and move the lamp seat to Left or
Right until it focus on the center of the slot. Then fix the screws.
If the facular deviate to Up and Down, then loosen the No.2 screws
and move the lamp seat Up and Down until the facular focus on the center
of the slot. Then fix the No. 2 screws again.

Right Position

Switch
Mirror
2

Step 7. Finish
Reset the cover of the light chamber and fix the screws. Reset the
cover of the instrument and fix the screws. Recover the Pole in the

19
 compartment, then the course finished.

Warranty
Wewarrant that this product will be free from defects in material and
workmanship for a period of one (1) year from date of purchase. If a defect
is present, we will, at its option, repair, replace, or refund the purchase
price of this product at no charge to you, provided it is returned during
the warranty period. This warranty does not apply if the product has been
damaged by accident, abuse, misuse, or misapplication, or from ordinary
wear and tear.

For your protection, items being returned must be insured against possible
damage or loss. This warranty shall be limited to the replacement of
defective products. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN
LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF
MERCHANTABILITY.

Equipment Disposal

This equipment is marked with the crossed out wheeled bin symbol to indicate
that this equipment must not be disposed of with unsorted waste.

Instead it's your responsibility to correctly dispose of your equipment

at lifecycle -end by handling it over to an authorized facility for separate
collection and recycling. It's also your responsibility to decontaminate
the equipment in case of biological, chemical and/or radiological
contamination, so as to protect from health hazards the persons involved
in the disposal and recycling of the equipment.

For more information about where you can drop off your waste of equipment,
please contact your local dealer from whom you originally purchased this

20
equipment.

By doing so, you will help to conserve natural and environmental resources
and you will ensure that your equipment is recycled in a manner that
protects human health.

Thank you!

21
 DLAB Scientific Inc.
Suite R, 5308 Derry Avenue, Agoura Hills, CA91301 California, United States.
Tel: +1-909-742-8958
Fax: +1-909-939-4212
Sales contact: [email protected]
Service contact: [email protected]
www.dlabsci.com