life

Article
Ocular Surface Changes Associated with Face Masks in
Healthcare Personnel during COVID-19 Pandemic
Filippo Tatti 1 , Lorenzo Mangoni 1 , Simone Pirodda 1 , Giuseppe Demarinis 1 , Claudio Iovino 1,2 ,
Emanuele Siotto Pintor 1 , Germano Orrù 3 , Luigi Isaia Lecca 4 , Marcello Campagna 4 , Gloria Denotti 5
and Enrico Peiretti 1, *

1 Department of Surgical Sciences, Eye Clinic, University of Cagliari, 09124 Cagliari, Italy
2 Multidisciplinary Department of Medical, Surgical and Dental Sciences, Eye Clinic, University of Campania
Luigi Vanvitelli, 80131 Naples, Italy
3 Molecular Biology Service Lab, Department of Surgical Science, University of Cagliari, 09124 Cagliari, Italy
4 Division of Occupational Medicine, Department of Medical Sciences and Public Health, University of Cagliari,
09042 Monserrato, Italy
5 Department of Surgical Science, Institute of Dentistry, University of Cagliari, 09124 Cagliari, Italy
* Correspondence: [email protected]

Abstract: The aim of this study was to investigate ocular surface changes associated with face mask
(FMs) use of healthcare personnel during the COVID-19 pandemic. We prospectively evaluated
200 eyes of 100 individuals during working hours and 40 eyes of 20 individuals during their rest
days as a control group. Dry eye symptoms were assessed with the Ocular Surface Disease Index
(OSDI) and McMonnies questionnaire. The clinical investigation included the best corrected visual
acuity (BCVA), corneal fluorescein staining (FS), break-up time (BUT), and Schirmer test I before
and after a 7-h work shift with a continuative use of surgical or N95 masks. The control group was
Citation: Tatti, F.; Mangoni, L.;
evaluated similarly twice a day, at 8:00 a.m. and at 3:00 p.m. In the study group, BCVA, FS, BUT,
Pirodda, S.; Demarinis, G.; Iovino, C.;
Siotto Pintor, E.; Orrù, G.; Lecca, L.I.;
and Schirmer test were investigated and there was a significant negative variation at the end of the
Campagna, M.; Denotti, G.; et al. shift. On the contrary, the control group did not show significant variations of any clinical feature.
Ocular Surface Changes Associated Furthermore, no significant changes in clinical parameters were observed during the use of surgical
with Face Masks in Healthcare or N95 masks. In conclusion, FMs continuative use resulted in daily ocular surface modifications
Personnel during COVID-19 specifically in healthcare personnel.
Pandemic. Life 2022, 12, 1491.
https://doi.org/10.3390/ Keywords: COVID-19; face mask; dry eye; mask-associated dry eye
life12101491

Academic Editor: Robert Gabriel

Received: 17 August 2022 1. Introduction

Accepted: 21 September 2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of
Published: 26 September 2022
2019, causing severe pneumonia in a number of patients in Wuhan, China. Subsequently,
Publisher’s Note: MDPI stays neutral it rapidly spread worldwide, leading the World Health Organization (WHO) to declare
with regard to jurisdictional claims in the related disease, named coronavirus disease 2019 (COVID-19), a worldwide pandemic
published maps and institutional affil- by the end of January 2020 [1]. In parallel with the vaccination campaign, proven to be
iations.
effective at preventing not only severe stages of the disease, but also patients’ hospitaliza-
tion and death, the global spread of SARS-CoV-2 led to the adoption of urgent measures
to contain its transmission [2–4]. However, while the imperative use of face masks (FMs)
played a pivotal role in avoiding the forward projection of expelled air and preventing
Copyright: © 2022 by the authors.
airborne virus transmission, several studies reported their significant contribution to the
Licensee MDPI, Basel, Switzerland.
This article is an open access article
worsening of dry eye disease (DED) symptoms [5–8] and, consequently, to the quality of
distributed under the terms and
life (QoL) [9,10]. Indeed, an increased prevalence of ocular surface modifications, ocular
conditions of the Creative Commons discomfort, and ocular surface homeostasis impairment due to prolonged use of FMs dur-
Attribution (CC BY) license (https:// ing the COVID-19 pandemic was highlighted in the most recent literature [11–14]. Indeed,
creativecommons.org/licenses/by/ the air expelled when wearing FMs and directed upwards through the top opening of the
4.0/).

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Life 2022, 12, 1491 2 of 11

mask [15–17] would wrap around the corneal surface, creating conditions that may acceler-
ate corneal tear film evaporation and lead to a new clinical entity called mask-associated
dry eye (MADE) [11,13]. A recent study identified other co-risk factors implicated in
DED pathogenesis in the COVID era including digital devices misuse, unbalanced diet,
insufficient hydration, sleep deprivation, and the psychological repercussions of pandemic
restrictions [5].
Under this light, considering the WHO recommendations on wearing FMs (N95, FFP2,
or FFP3) in healthcare settings [18] and the work shifts of health workers (about 7 h/day in
Italy), these individuals may potentially be at high risk for the occurrence or worsening
of DED. Moreover, even though mask-associated dry eye has already been demonstrated,
information on daily ocular surface modifications still remains unknown. Therefore, the
aim of this study was to investigate FMs-related ocular surface changes in healthcare
personnel, using questionnaires and clinical examinations.

2. Materials and Methods

2.1. Subjects
This was a prospective study conducted at the University Hospital of Cagliari, Italy,
during the COVID-19 pandemic. A total of 100 health workers who wore FMs continuously
during their work shifts were enrolled from April to June 2021, for a total of 200 eyes
examined. The cohort consisted of 56 physicians and 44 nurses from the ophthalmologic,
dermatologic, and occupational health units and the microbiology laboratory. All subjects
were evaluated before work, between 07:00 and 08:00 a.m., and at the end of the shift,
between 2:00 and 3:00 p.m. Each subject confirmed the continuative use of surgical or
FFP2/N95 FMs with ear loops (both made by BYD Precision Manufacture Co. Ltd., Shen-
zhen, China) and allocated into 2 groups based on the FM type used. A control group
of forty eyes of 20 health workers was evaluated twice a day, at 8:00 a.m. and 3:00 p.m.,
during their rest days.
The exclusion criteria included previous ocular surgery, a history of systemic or in-
traocular disease, use of topical therapies during work that could modify the ocular surface,
and smoking habit. The study adhered to the tenets of the Declaration of Helsinki and
the protocol used was approved by the local Institutional Review Board (PG/2021/5479).
Informed consent was obtained before enrolment from all participants.

2.2. Questionnaire
All subjects were evaluated for DED at baseline with the Ocular Surface Disease Index
(OSDI) and the McMonnies questionnaire. The OSDI is a 12-item scale created to assess
subjective dry eye symptoms and the effects of the disease on vision-related activities of
daily living within the previous week. The total OSDI score ranges from 0 to 100 points
and positively correlates with DED severity, with a cut-off value of 13 for a diagnosis
of DED [19]. The McMonnies questionnaire is a 12-item instrument for the screening
of dry eye disease. Each question is individually scored and the McMonnies index is
calculated by individually summing them up (perfect score = 45). The severity of dry eye
symptoms correlates with this index, with 14.5 points as the cut-off value for a diagnosis of
DED [19]. All participants were also asked about other co-risk factors, such as exposure to
air-conditioning and the use of visual display terminals (VDTs) [9].

2.3. Clinical Examination

The clinical tests performed included in sequence best-corrected visual acuity (BCVA),
anterior segment slit-lamp examination, corneal fluorescein staining (FS), tear film break-up
time (BUT), and Schirmer test I. BCVA was measured by using a 3-m Snellen chart and
then converted to LogMAR. FS was evaluated according to the Oxford Grading Scale
(OGS) on the basis of the fluorescein dye staining pattern on an ocular surface [20]. BUT is
evaluated as the timespan, after fluorescein dye application, between a complete blink and
the appearance of the first dry spot on the corneal surface. Schirmer test I is performed by
Life 2022, 12, 1491 3 of 11

folding a Schirmer paper strip at the notch and hooking the folded end over the temporal
one-third of the lower lid margin. The score is then measured as the length of wetting from
the notch after 5 min with the eyes gently closed. In accordance with previous studies, we
considered as pathological a cut-off of 10 s and 10 mm/5 min for BUT and Schirmer test I,
respectively [21].

2.4. Evaluation Method

All subjects were evaluated by 2 independent observers (F.T. and S.P.), who collected
all clinical data as well. The series of examinations lasted approximately 15 to 20 min for
each patient. The concordant data were assumed as final data; conversely, a mean value
was calculated from the discordant data. If one of the observers was not present at the
time of the assessment, the visit was recorded on video and subsequently evaluated by the
second observer.

2.5. Statistical Analysis

Data were recorded on Microsoft Office Excel (Microsoft Corporation, Redmond, WA,
USA) and the statistical analysis was performed using Excel and SPSS Statistics v28.0
(IBM Corp., Armonk, NY, USA). The Kolmogorov–Smirnov test was used to evaluate the
normal distribution for each variable. A paired t-test or Wilcoxon rank sum test was used
to compare continuous variables before and after the shift. A mixed ANOVA was used
to compare continuous variables before and after the shift, with the type of mask used as
the between-subjects factor. Multiple regression analysis was performed to investigate the
relationship between demographic data and parameter changes. Any p-values less than
0.05 were considered statistically significant.

3. Results
A total of 200 eyes of 100 health workers (44 men and 56 women) who wore FMs at
work were included. Their mean age was 44.56 years (±14.87). The study participants were
evaluated by the OSDI and McMonnies questionnaire to make an accurate assessment of
DED. An amount of 33 subjects exceeded the cut-off value of 13 for the OSDI screening,
whereas 19 subjects showed positive results (score > 14.5) on the McMonnies questionnaire.
The control group of 40 eyes of 20 subjects (8 males and 12 females) showed a mean age of
40.9 (±13.93). All demographic data of the study participants are summarized in Table 1.

Table 1. Demographic characteristics of the sample of health workers.

Study Group = 100 Control Group = 20

Parameter Value SD Value SD p
Mean age (years) 44.56 14.87 40.9 13.93 0.069
Sex
M 44 (44%) 8 (40%) 0.74
F 56 (56%) 12 (60%)
Mean OSDI 12.41 12.06 12.57 12.78 0.82
Mean McMonnies 10.22 5.25 11.28 5.38 0.53
Mean VDT use (h) 0.60 0.95 0.7 0.86 0.47
Glasses/eye protection
Yes 49 (49%)
No 51 (51%)
SD, standard deviation; VDT, video display terminal.

3.1. Clinical Parameters

The clinical data collected before and at the end of the work shift are reported in
Table 2. Overall, BCVA, BUT (Figure 1), Schirmer test, and FS showed significantly worse
values at the end of the shift (all p < 0.001).
 Life 2022, 12, x FOR PEER REVIEW 4 of 11

Life 2022, 12, 1491 3.1. Clinical Parameters 4 of 11

The clinical data collected before and at the end of the work shift are reported in Table
2. Overall, BCVA, BUT (Figure 1), Schirmer test, and FS showed significantly worse values
at the
Table end of parameters
2. Clinical the shift (all p < 0.001).
collected before and at the end of the work shift.

Table Parameter
2. Clinical parameters collected
Before Workbefore SD
and at theEnd
endofofWork
the work shift.
SD p-Value
Shift Shift
Parameter Before Work Shift
Mean BCVA (LogMar) 0.05 SD0.09 End 0.07
of Work Shift
0.10 SD<0.001p-value
Mean BCVA (LogMar) Mean Schirmer test value0.05 0.09 0.07 0.10 <0.001
16.16 8.90 14.04 9.70 <0.001
Mean Schirmer test value (mm) (mm) 16.16 8.90 14.04 9.70 <0.001
Mean BUT (seconds) Mean BUT (seconds) 9.15 9.15 2.67
2.67 7.497.49 2.53 2.53<0.001<0.001
Mean FS (OGS) Mean FS (OGS) 0.10 0.10 0.35
0.35 0.510.51 0.66 0.66<0.001<0.001
BCVA, best corrected
BCVA, visual acuity;
best corrected visualBUT, break-up
acuity; BUT,time; FS, Fluorescein
break-up staining;
time; FS, OGS, Oxford
Fluorescein Grading
staining; Scale;
OGS, SD,
Oxford
standard deviation.
Grading Scale; SD, standard deviation.

(a) (b)

(c) (d)
Figure
Figure 1. Variations
1. Variations in break-up
in break-up time
time (BUT)
(BUT) of aof50-year-old
a 50-year-old female
female health
health worker.
worker. Representative
Representative
slit-lamp frames are from a BUT video of case 44. The first examination (08:05) shows (a) (a)
slit-lamp frames are from a BUT video of case 44. The first examination (08:05) shows stable
stable tear
tear
film after blinking at the start of the measurement area and (b) the development of dark
film after blinking at the start of the measurement area and (b) the development of dark areas at 7 s. areas at 7
s. The second examination (14:05) shows (c) stable tear film at the start of the measurement area and
The second examination (14:05) shows (c) stable tear film at the start of the measurement area and
(d) the development of bigger dark areas at 5 s (d).
(d) the development of bigger dark areas at 5 s (d).

There
There were
were notnot
anyany significant
significant differences
differences between
between thethe pre-shift
pre-shift clinical
clinical parameters
parameters
of the control group and the pre-shift clinical parameters of the study group (all (all
of the control group and the pre-shift clinical parameters of the study group p>0.05)
p > 0.05)
(Table
(Table 3). 3).

Table
Table 3. Clinical
3. Clinical parameters
parameters collected
collected at 8:00
at 8:00 a.m. AM
in the in the
control control
group group
and in andgroup.
the study in the study
group.
p-Value
Pre-Shift Parameter Pre-Shift Parameter
Control GroupControl Group Study Group
Study Group p-value
Mean BCVA (LogMar)Mean BCVA (LogMar) 0.025 0.025 0.05
0.05 0.06 0.06
Mean(mm)
Mean Schirmer Test Value Schirmer Test Value (mm)
18.68 18.68 16.16
16.16 0.12 0.12
Mean BUT (seconds) 10.38 9.15 0.24
Mean FS (OGS) 0.15 0.1 0.22
BCVA, best corrected visual acuity; BUT, break-up time; FS, Fluorescein staining; OGS, Oxford Grading Scale; SD,
standard deviation.
Life 2022, 12, 1491 5 of 11

As shown in Table 4, the control group showed no significant variation (p > 0.05) of
any clinical parameter (BCVA, BUT, Schirmer test, and FS).

Table 4. Clinical parameters collected in the control group.

8:00 a.m. 3:00 p.m.

Parameter SD SD p-Value
Examination Examination
Mean BCVA (LogMar) 0.025 0.07 0.01 0.03 0.09
Mean Schirmer Test Value (mm) 18.68 6.94 18.35 7.34 0.69
Mean BUT (seconds) 10.38 3.64 10.25 3.40 0.59
Mean FS (OGS) 0.15 0.36 0.2 0.40 0.15
BCVA, best corrected visual acuity; BUT, break-up time; FS, Fluorescein staining; OGS, Oxford Grading Scale; SD,
standard deviation.

3.2. DED Diagnosis

The BUT test showed a pathological score of 104 and 153 for eyes before and after
work, respectively. With regards to the Schirmer test, it demonstrated an increase in the
number of eyes affected from 50 to 76, before and after the work shift, respectively.
Stratifying the cohort on the basis of BUT and Schirmer test severity and assessing the
data before and after the 7-h shift, significant differences were observed (p < 0.005) with
substantially worse test results at the end of the 7-h shift.
In accordance with the Dry Eye Workshop II guidelines, the presence of dry eye
symptoms (OSDI score ≥ 13) with at least one homeostasis test resulting positive, such as
BUT < 10 s, allows diagnosis of DED [21].
Considering only the shorter BUT of both the eyes of the 33 subjects with a positive
result on OSDI screening (score ≥ 13), a clinical diagnosis of DED could be made for 21
and 32 individuals before and after the work shift, respectively (Table 5).

Table 5. Study group for the dry eye disease assessment. In brackets, tests results are expressed in
mean (±SD).

N. Eyes (Mean Values ± SD)

Schirmer Test Pre-WS Post-WS
N. Eyes: 50 N. eyes: 76
<10 mm/5 m
(4.9 ± 1.56) (4.32 ± 4.51)
N. Eyes: 9 N. Eyes: 39
<5 mm/5 m
(2.44 ± 1.24) (1.56 ± 1.33)
BUT
N. Eyes: 104 N. Eyes: 153
<10 s
(7.01 ± 1.65) (6.17 ± 1.97)
N. Eyes: 95 N. Eyes: 130
5–10s
(7.35 ± 1.26) (6.46 ± 1.79)
N. Eyes: 9 N. Eyes: 23
<5 s
(3.44 ± 0.72) (3.11 ± 0.92)
Subjects
DED symptoms
33
(OSDI score ≥ 13)
Mild (13–22) 17
Moderate (23–32) 8
Severe (33–100) 8
DED Diagnosis
21 32
(OSDI score ≥ 13 and BUT < 10 s)
N., Number of; SD, standard deviation; WS, work shift; DED, dry eye disease; BUT, break-up time.

3.3. Type of Mask

There were no significant differences between the groups in terms of age, gender,
OSDI, McMonnies, time of VDT use, and use of glasses (Table 6).
 21 32
(OSDI score 13 and BUT <10 s)
N., Number of; SD, standard deviation; WS, work shift; DED, dry eye disease; BUT, break-up time

3.3. Type of Mask

Life 2022, 12, 1491 There were no significant differences between the groups in terms of age,6gender,
of 11
OSDI, McMonnies, time of VDT use, and use of glasses (Table 6).

Table 6. Demographic parameters of surgical mask and N95 groups.

Table 6. Demographic parameters of surgical mask and N95 groups.
Surgical Mask Group (n = 40) N95 Group (n = 60) p-value
Surgical Mask Group (n = 40) N95 Group (n = 60) p-Value
Age (y) 45.2 ± 14.7 44.1 ± 15.1 0.72
Gender (M:F) Age (y) 20:20 45.2 ± 14.7 44.1 ± 15.1
24:36 0.72 0.32
Gender (M:F) 20:20 24:36 0.32
OSDI OSDI 9.5 ± 9.2 9.5 ± 9.2 13.8 ± 13.6
13.8 ± 13.6 0.06 0.06
McMonnies McMonnies 9.2 ± 4.7 9.2 ± 4.7 10.9 ± 5.5
10.9 ± 5.5 0.10 0.10
VDT use (h) VDT use (h) 0.7 ± 1.1 0.7 ± 1.1 0.60.6 ± 0.8
± 0.8 0.61 0.61
Wearing glasses Wearing glasses 17 17 34 34 0.16 0.16
VDT, video display terminal.
VDT, video display terminal.

In both groups,
In both therethere
groups, was awas
significant difference
a significant between
difference clinical clinical
between parameters collectedcol-
parameters
before
lected before and after the shift (p < 0.05). We also performed a comparative between
and after the shift (p < 0.05). We also performed a comparative analysis analysis be-
the tween
groupstheforgroups
BCVA,for Schirmer, BUT, andBUT,
BCVA, Schirmer, OGS and
score variance
OGS after working
score variance with FMs
after working with
(Figure 2).
FMs (Figure 2).

(a) (b)

Figure 2. Variations in clinical parameters after continuative use of surgical mask or respirators
Figure
during 2. Variations
work. in clinical
The bar graph parameters
(a,b) illustrates after
values continuative
of clinical use of(Schirmer,
parameters surgical mask or respirators
BUT, BCVA and
during work. The bar graph (a, b) illustrates values of clinical parameters (Schirmer, BUT, BCVA
OGS); bars represent mean values and standard errors (SE) in surgical mask group (green) and N95
and OGS); bars represent mean values and standard errors (SE) in surgical mask group (green) and
group (blue) as indicated in Table 7.
N95 group (blue) as indicated in Table 7.

Table 7. Variations
Table in clinical
7. Variations parameters
in clinical after continuative
parameters use of surgical
after continuative mask or N95
use of surgical maskduring
or N95work.
during
work.
Variations in Clinical Parameters
Schirmer (mm)
Variations
BUT (s)
in Clinical Parameters
BCVA (LogMar) FS (OGS)
FACE
Schirmer (mm) BUT (s) BCVA (LogMar) FS (OGS)
MASK PRE- POST-
SE PRE-
PRE-
SE POST- SE PRE-
POST-
SE
PRE-
SE
POST-
SE
PRE-
SE
POST-
SE
FACE MASK
WS WS WS WS POST- WS PRE- WS POST- WS PRE- WSPOST-
TYPE SE SE SE SE SE SE SE SE
Surgical
TYPE WS WS WS WS WS WS WS WS
16.14 0.94 13.05 1.02 8.86 0.28 7.06 0.25 0.06 0.01 0.07 0.01 0.1 0.04 0.43 0.07
Surgical Mask 16.14 0.94 13.05 1.02 8.86 0.28 7.06 0.25 0.06 0.01 0.07 0.01 0.1 0.04 0.43 0.07
Mask
N95 16.18 0.85 14.70 0.92 9.34 0.26 7.78 0.24 0.04 0.01 0.06 0.01 0.1 0.03 0.55 0.06
N95 16.18 0.85 14.70 0.92 9.34 0.26 7.78 0.24 0.04 0.01 0.06 0.01 0.1 0.03 0.55 0.06
BCVA, best corrected visual acuity; BUT, break-up time; FS, Fluorescein staining; OGS, Oxford Grading Scale; WS,
work shift.

As shown in Table 8, no significant differences between surgical masks and N95s were
observed (p > 0.05).
Life 2022, 12, 1491 7 of 11

Table 8. ANOVA with repeated measures analysis shows a statistically significant difference between
variables pre/post continuative use of FMs (p < 0.001). Face mask type (surgical or respirator) had no
statistically significant effect on clinical parameters after work (p > 0.05).

Test of within-Subjects Effect

Schirmer (mm) BUT (s) BCVA (LogMar) FS (OGS)
SOURCE F (1,198) p-Value F (1,198) p-Value F (1,198) p-Value F (1,198) p-Value
Pre/Post Work Shift 23.54 <0.001 132.60 <0.001 23.60 <0.001 12.16 <0.001
Face Mask Type 0.48 0.49 2.57 0.111 0.69 0.41 2.79 0.96
FMs, face masks; BCVA, best corrected visual acuity; BUT, break-up time; FS, Fluorescein staining; OGS, Oxford
Grading Scale.

3.4. Correlations
Multiple regression analysis between demographic and clinical variations was per-
formed, with weakly significant results (Table 9). The analysis of variance showed that
BCVA variation was negatively correlated with age (R = −0.23; p = 0.0047) and OSDI score
(R = −0.20; p = 0.0303), and positively correlated with male sex (R = 0.15; p = 0.0187). BUT
variation was weakly correlated with time of VDT use (R = −0.08; p = 0.0469). All other
correlations were not significant.

Table 9. Multiple regression analysis between demographic and clinical parameters.

McMonnies VDT Use Wearing

Parameter Age (y) Gender OSDI Score
Score Time (h) Glasses
BCVA Variation −0.23 0.15 −0.20 −0.13 −0.02 −0.07
(LogMar) (p = 0.0047) (p = 0.0187) (p = 0.0303) (p = 0.1333) (p = 0.7077) (p = 0.1975)
Schirmer Test Variation 0.09 −0.11 0.05 0.04 0.12 −0.019
(mm) (p = 0.2212) (p = 0.2265) (p = 0.1921) (p = 0.7628) (p = 0.1543) (p = 0.6985)
−0.08 −0.04 0.02 −0.12 −0.08 0.05
BUT Variation (s)
(p = 0.0833) (p = 0.6917) (p = 0.5643) (p = 0.2213) (p = 0.0469) (p = 0.6157)
−0.11 0.06 0.11 0.08 −0.05 −0.11
FS Variation (OGS)
(p = 0.0859) (p = 0.4098) (p = 0.3813) (p = 0.1472) (p = 0.1970) (p = 0.1019)
Y, years; s, seconds; mm, millimetres; h, hours; BCVA, best corrected visual acuity; BUT, break-up time; VDT,
video display terminal.

4. Discussion
Although the continuative use of FMs is essential to control the COVID-19 pandemic’s
spreading, it may influence ocular surface health owing to air leaking around the mask’s
edge during inhalation and exhalation [8,11,13,14,22].
In our study, we investigated ocular surface changes through standard tests, including
BCVA, BUT, Schirmer test, and FS [9,23]. In particular, we analysed the variations in these
parameters in healthcare workers after a 7-h shift with continuous use of FMs.
Giannaccare et al. suggested that the role of FMs in ocular discomfort symptoms is due
to the rapid tear evaporation caused by air dispersion around the mask [11]. Considering
that DED is clinically divided into two subtypes based on tear production (deficiency of
aqueous or hypo-secretive component) or evaporation (hyper-evaporative) [9], MADE may
be included in the second category.
As previously reported, high airflow may lead to water evaporation from the pre-
corneal tear film with resulting dry spots formation [24–26]. Indeed, a significant decrease
in lower tear meniscus dimensions was shown in subjects with a baseline short BUT (<5 s)
after exposure to an airflow of 1.5 m/s [26]. On the other hand, the airflow effect in healthy
eyes is controversial. Some authors demonstrated an increase of lower tear meniscus
dimensions as a result of reflex sensory loop and tear secretion compensation induced
Life 2022, 12, 1491 8 of 11

by airflow exposure changes in the tear film [26]. In contrast, Wyon et al. reported that
an exposure of the tear film to high air velocity (1.0 m/s) for 30 min led to a significant
decrease in tear stability, as demonstrated by reduced BUT in healthy eyes [24].
Similarly, in our study, there was evidence of significant worsening of the clinical
parameters analysed in the entire cohort after wearing FMS continuously during work and
exposing the tear film to the related abnormal airflow.
Given the need to wear a FM throughout the whole shift, the ocular surface of health-
care workers’ eyes is subjected to limited airflow for a long time. This condition of chronic
ocular surface insult could lead to long-term damage of tear film stability, as demonstrated
by Mastropasqua et al. In fact, the continuative use of FMs for more than 6 h/day during a
3-month follow-up, worsened clinical indicators of ocular surface disease and increased
cellular and molecular inflammation bio-markers [14]. These signs of ocular surface dam-
age, seen in healthy subjects, were more severe in patients with DED even when they used
FMs for less than 6 h/day [14].
The present study shows daily ocular surface changes in healthcare personnel after
continuous use of FMs for about 7 h, and we supposed that these daily changes could lead
to long-term damage, as shown in the previous study by Mastropasqua et al. [14].
It is important to highlight that the analysis of clinical parameters before and after the
work shift could be affected by a physiological modification of tear film during the day,
which has been, however, a particularly controversial area of research [27–31]. Oncel et al.
reported that tear osmolarity does not have diurnal oscillations in normal subjects [27].
Similarly, no significant differences were found between morning and evening BUT mea-
surements or in tear ferning test patterns at different hours of the day [28,29]. On the
contrary, a daily decrease in tear meniscus volume by tear strip meniscometry was reported
by Ayaki et al. [30]. A study by Lira et al. [31] showed that tear film quantity, analysed by
Schirmer measurements and tear meniscus height, is unrelated to the hour it is assessed,
while there is worsening of tear film quality evaluated by BUT and non-invasive BUT. In
those studies [28–31], the authors suggested that the behaviour of tear film is different
between patients with diagnosed DED and normal subjects. This concept was in fact
confirmed by comparing visual function in the two groups. Walker et al. [32] reported that
individuals with dry eyes experience significant diurnal fluctuations in visual function
and in signs and symptoms of their condition due to an increased rate of keratitis in the
evening, whereas previous research on normal subjects demonstrated an increase in visual
function ability from morning to evening [33]. Our results are consistent with this finding,
showing a BCVA worsening at the end of the shift, with wearing FMs possibly playing a
pivotal role in that phenomenon.
Although all FMs are effective at slowing down the frontward flow and the horizontal
distance travelled by aerosol particles [15,16], a surgical mask not fitting the face tightly
causes greater air leakage around the sides during coughing [15], whereas an upward
leakage jet was described for FFP2 masks when it was not possible to seal them properly [15].
In our study, no demographic differences were found between the groups of surgical masks
and N95 wearers. Nevertheless, a worsening of the clinical parameters was found in both
the groups at the end of the work shift that we supposed to be related to an upward
dispersion of exhaled air. We suggest that the mask should be carefully shaped to the nose
to ensure a proper seal and, in turn, to reduce the airflow over the corneal surface.
Although Krolo et al. [12] observed a higher incidence of MADE in women and
subjects with a previous history of DED who wore FMs longer than 3 h/day, in the present
study, we did not find any significant correlation either between parameter variations and
demographic data nor between dry eye assessment (OSDI and McMonnies questionnaire)
and ocular surface changes. However, it is interesting to note that among the 33 healthcare
workers with dry eye symptoms, 21 showed BUT impairment (<10 s) before and after work
while 11 of them showed impairment only after work. Only one individual did not show
any homeostasis biomarker alteration.
Life 2022, 12, 1491 9 of 11

With regards to the use of goggles or eye protection devices, these were reported
to increase periocular humidity and therefore could be a protective measure for dry eye
occurrence or worsening [34,35]. However, in the present study, wearing glasses or eye
protection equipment was found to not influence the variation of parameters explored.
WHO estimates that between 80,000 and 180,000 healthcare workers died from COVID-19
between January 2020 and May 2021, converging to a medium scenario of 115,500 deaths [36].
The severity of the complications related to COVID-19 underlines the importance of using
preventive measures, especially in healthcare settings. Indeed, face masks are an essential
tool for healthcare personnel, and their use is mandatory due to the more concerning
consequences of SARS-COV-2 infection more than dry eye symptoms [18]. Neverthe-
less, long-term mask wearing has created a new problem to cope with, albeit of minor
importance.
Defining an occupational disease involves two main elements: the exposure–effect
relationship between a specific working environment and/or activity and a specific disease
effect, and the fact that these diseases occur among certain groups with a frequency higher
than the average for the rest of the population [37]. Further study is required to define
whether those criteria will be met and affect the magnitude of DED in mask wearers
worldwide.
To our knowledge, the present is the first study to focus on ocular surface daily
modifications in healthcare personnel wearing FMs. The short-term follow-up better
reflects the ocular surface damage induced by only using FMs.
Limitations of the current study include the lack of close monitoring of the subjects
during the work shift to ensure proper mask sealing. In addition, study participants
were asked about the use of video terminals, but no specific environment information
were evaluated: further study should be directed to also investigate humidity levels and
room lighting of each workday environment. Furthermore, the two study arms were
not homogeneous in terms of number: the control group of subjects not using FMs was
smaller than the study group. Another limitation was that we exclusively assessed the FMs’
influence with standard tests of DED, while a tear meniscus analysis with optical coherence
tomography, osmolarity testing, and the identification of pro-inflammatory markers may
add useful information.
Further population studies involving larger cohorts will be required to better define
the actual incidence and severity of DED in mask wearers worldwide. Given the intensive
FMs use, nowadays compulsory in many different settings, and the fact that DED often
remains unrecognized until symptoms are significantly advanced [7], a DED assessment
to evaluate the need for therapy should be performed as part of work health control. On
the other hand, healthcare personnel should observe a series of precautions to reduce the
impact of FMs on their ocular surfaces, such as ensuring the mask to be properly sealed onto
the nose reducing the airflow turbulence towards the eyes. In addition, following simple
healthy lifestyle measures, such as drinking an adequate amount of water to counteract the
drying process, may be appropriate.
Furthermore, considering the difficulty of predicting the duration of anti-pandemic
measures, a longitudinal examination of DED signs and symptoms in healthy subjects and
DED patients would be desirable to evaluate the long-term impact of these measures on
ocular surfaces.

5. Conclusions
FMs are an essential tool for healthcare personnel, and their use is mandatory to
avoid concerning consequences of SARS-COV-2 infection [18]. However, wearing them
for long-term periods, especially in work environments, has created several minor issues,
including modifications to the ocular surface and clinical signs of DED. The worsening of
clinical parameters did not correlate with the type of FM worn. Further studies are required
to avoid potential biases and identify ocular surface inflammation markers.
Life 2022, 12, 1491 10 of 11

Author Contributions: Conceptualization, F.T. and E.P.; formal analysis, F.T. and L.M.; investigation,
F.T. and S.P.; methodology, F.T., L.M., G.D. (Giuseppe Demarinis) and E.P.; supervision, G.O., M.C.,
G.D. (Gloria Denotti) and E.P.; writing—original draft, F.T., L.M. and E.P.; writing—review and
editing, G.D. (Giuseppe Demarinis), E.S.P., C.I., L.I.L. and E.P. All authors have read and agreed to
the published version of the manuscript.
Funding: This research received no specific grant from any public funding agency, or from the
commercial or not-for-profit sector.
Institutional Review Board Statement: The study was approved by the Local Institutional Review
Board on 31 March 2021 (PG/2021/5479). All procedures were performed in accordance with the
principles of the Declaration of Helsinki.
Informed Consent Statement: Written informed consent was obtained from each participant.
Data Availability Statement: Not applicable.
Conflicts of Interest: The authors declare no conflict of interest.

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