Test Kit Instructions

Quench-GoneTM Organic Modified Test Kit

Product #: QGOM-25 / QGOM-100

Introduction Choosing the Right Test Kit

At LuminUltra, we are committed to providing high LuminUltra provides 6 core test kits for measuring total
quality test kits to anyone that needs fast and reliable microbiological concentration via ATP, each tailored to
results about the microbiological characteristics of any specific applications:
process! Visit www.luminultra.com to learn about the
• Quench-Gone Aqueous (QGATM):
exciting opportunities that our solutions can provide.
For low-solids water-based samples, such as
Whereas traditional microbiological tests require days drinking, cooling and process waters with less than
for feedback and measure only a fraction of the 10% free oil and/or salinity.
microorganisms, 2nd Generation ATP® (Adenosine
• Quench-Gone Organic Modified (QGO-MTM):
Triphosphate) test kits from LuminUltra measure total
For low-solids organic-based samples, such as
microorganisms and provide feedback in minutes!
fuel, bottom waters, metalworking fluids, lubricants,
In this test kit instruction guide, you will learn... oily brine, and oilfield waters with more than 10%
free oil and/or salinity.
• Where this kit can be used;
o QGOM-XLPD is available for difficult-to-
• How 2nd Generation ATP technology works;
filter samples such as polymers.
• How to handle and store components of this kit;
o dQGO-M™ is also available for separate
• How to perform tests; determination of large and small cells.

• How to calculate and interpret results; and • Deposit & Surface Analysis (DSATM):
For measuring attached growth such as biofilm,
• How to contact us.
corrosion products, slimes, and biological filter
media.
• QuenchGone21TM Industrial (QG21ITM):
For high-solids process fluids, including paper
process and other wash waters.
• QuenchGone21 Specialty (QG21STM):
For chemical product testing, such as slurries,
adhesives, paints, and other coatings.
• QuenchGone21 Wastewater (QG21WTM):
For wastewater and bioprocessing samples,
whether influent, bioreactor or effluent. Also
QGO-M Test Kit (QGOM-100C) provides the capability to quantify attached growth
and floc bulking sedimentation processes.

LuminUltra Technologies Ltd. © LuminUltra 2017

520 King Street Date Modified: 30-Jan-2017
Fredericton, New Brunswick, Canada E3B 6G3
Tel: +1-506-459-8777 Page 1 of 8
www.luminultra.com
Test Kit Instructions – Quench-Gone Organic Modified

NOTE: The QGOM-XLPD method is optimized to measure

Where to Use This Test Kit
down to 2 pg ATP/mL.
NOTE: The QGO-M test kit is compliant with ASTM Standard
E 2694 for the measurement of ATP in Metalworking Fluids What Else is Required?
and D 7687 for the measurement of ATP in fuels, fuel/water
mixtures and fuel-associated water. LuminUltra’s test kits contain all of the consumable
The Quench-Gone Organic Modified (QGO-M) test kit materials required to run their specified number of tests
is designed for low-solids organic-based samples. (Defined by the last 2 or 3 digits of the product code).
Using a single analysis, you will be able to quickly To use our test kits, you will require a luminometer.
measure total microbiological concentration in any For optimal functionality, we recommend the
organic sample with a wide detection range. Use PhotonMaster™ Luminometer & Bluetooth Module
QGO-M to detect total microbiological activity in: Equipment Set (EQP-PBM-PAC), which includes a
Carry Case, Micropipettors, PhotonMaster
 Metalworking Fluids  Finished Fuels Luminometer, PhotonMaster Bluetooth Module, and
 Hydrocarbon Diluents  Lubricants Test Tube Racks.

 Crude Oil  Oily Brines

 Oilfield waters  Bottom Water
…and more! In general, the QGO-M test kit is used in
samples having more than 10% free oil and/or salinity
content. For samples with less than 10% free oil
and/or salinity, use the Quench-Gone Aqueous (QGA)
test kit. For samples that are more difficult to filter such
as latex polymers, concrete admixtures, and personal
or home care products, use the QGOM-XLPD method.
PhotonMaster & PBM Equipment Set (EQP-PBM-PAC)
How Does This Test Kit Work?
NOTE: LuminUltra’s test kits can be used with the majority of
LuminUltra’s test kits are based on the measurement photomultiplier tube–based luminometers, including the
of ATP, which is a direct and interference-free indicator Kikkoman Lumitester C-110 or Berthold Junior 9509LB.
of total living biomass. ATP is measured using the Contact LuminUltra to confirm compatibility of your
luminometer.
firefly luciferase assay, where a sample containing
ATP is introduced to a solution containing the enzyme We also recommend LuminUltra Cloud™,
Luciferase, which naturally occurs in the tails of a powerful mobile platform that allows you
fireflies, to produce light. The light is detected in a to calculate, store, and analyze your data
luminometer as Relative Light Units (RLU). with ease via your smartphone, tablet, or
Mg + + personal computer.
ATP + O 2 + luciferin luciferase
 → AMP + PPi + oxyluciferin + light
Through robust analytics, collaboration, and
The QGO-M test kit utilizes a 5-minute filtration-based reporting functions, users and their
analysis to measure a parameter called Cellular ATP colleagues can stay on top of their process
(cATP™). cATP represents ATP from living anytime, anywhere.
microorganisms in suspension in a fluid and therefore
Visit www.luminultra.com for instructions on
is a direct indication of the planktonic population.
how to acquire LuminUltra Cloud.
While QGO-M is optimized to measure down to 0.25
pg ATP/mL using standard procedures and LuminUltra is sensitive to the needs of each individual
equipment, there is essentially no limit to the sensitivity customer. We can supply you with on-site auditing and
of this method. Procedural adaptations can be used to training services, web-based training, and one-on-one
amplify the low-range sensitivity when necessary. consultation to get your process improvement program
Contact LuminUltra for more details on these options. off the ground. Contact us today to learn more!

www.luminultra.com © LuminUltra 2017 Page 2 of 8

 Test Kit Instructions – Quench-Gone Organic Modified

filters are recommended for use in all QGO-M analyses,

Test Kit Contents and Storage
although regular Quench-Gone filters may be used to test
When you receive your test kit, utilize the following less aggressive fluids such as oil-contaminated water.
guidelines for material storage. Note that the presence NOTE: QGOM-XLPD test kit contains LuminaseXL in place of
and quantity of each item listed below will depend on Luminase and additional Dilution Tubes for the pre-dilution
test kit size and type. Avoid freezing of all product step.
components except where noted, and avoid usage of
expired test kit components. General Tips
QGO-M Test Kit Contents & Storage Conditions
• New to 2nd Generation ATP technology? Before
Shelf getting started, consult www.luminultra.com for
Component (LuminUltra P/N) Storage
Life
TM
links to video demonstrations, use guidelines,
Luminase Enzyme & Buffer Vials*
4 to validation guidelines, other product documentation,
(Lu-3mL-FD) 24 mo**
25oC
Luciferase Enzyme Reagent, 3mL and more!
UltraCheck TM 1 Dropper Bottle***
4 to
(UC1-5mL)
25oC
24 mo • Microbiological characteristics of most samples will
1 ng ATP/mL Standard, 5mL
UltraLyse TM 7 Bottle begin to change immediately upon collection. If
4 to
(UL7-125mL)
25oC
24 mo samples cannot be tested within 2 hours of
ATP Extraction Reagent, 125mL
UltraLuteTM (Dilution) Tube, 9mL
collection, store refrigerated (2 to 8oC) and test
4 to
(ULu-9mL-50R) 24 mo within 24 hours of collection. Allow samples to
25oC
ATP Dilution Reagent, 9mL reach ambient temperature prior to testing, and
LumiCleanTM Bottle
4 to perform ATP analyses on the same sample used
(LC-250mL) 24 mo
25oC
Filter Wash Reagent, 250mL for measuring other parameters for reliable
Quench-Gone Organic Syringe Filters, 25/pk interpretation.
- -
(DIS-SFQGO-25)****

20mL Syringe, PP/PP, 25/pk

• Waste reagent can be discarded as general waste.
- -
(DIS-S20-25) Consult MSDS for more information. Contact
60mL Syringe, PP/Neoprene, 5/pk LuminUltra for copies of MSDS.
- -
(DIS-S60-5)
• All materials in this test kit including pipet tips and
100 to 1250µL Blue Pipet Tips, 96/rack test tubes are single-use only. Because ATP and
- -
(DIS-PT1-96R)
microorganisms are present on skin, do not touch
1 to 200µL Yellow Pipet Tips, 96/rack the surface of pipet tips. Ensure that all pipet tips
- -
(DIS-PT01-96R)
and test tubes are clean inside and outside prior to
12x55mm Test Tubes, 50/pk
(DIS-CT1255-50)
- - use. Do not mark on assay tubes as this may
impact light detection by the luminometer.
Disposable Bulb Pipets, 5mL, 25/Pack
- -
(DIS-BP5-25) • Note that the fixed-volume pipets included in the
* Note that the Luminase supplied in QGO-M kits is NOT
PhotonMaster & PBM Equipment Set cannot be
interchangeable with other forms of Luminase (i.e. recalibrated and should be replaced annually.
LuminaseW, Luminase Lite, and LuminaseXL). • Avoid taking multiple luminometer readings on the
** Luminase is manufactured and shipped as a set of vials, same assay. The light output from ATP assays is
one of each freeze-dried pellet and liquid buffer. The stated relatively constant and at a maximum for the first
shelf life reflects the freeze-dried form; store refrigerated for 15-30 seconds after mixing, after which the output
the best possible shelf life. Following rehydration, the will decline.
reagent will be stable for 3 months when refrigerated and 6
months when frozen. • When testing samples that yield low RLU values
(i.e. RLU ATP ≤ 50), it is recommended to account
*** Sufficient UltraCheck 1 and Luminase is included to
perform 1 standardization (Step 1) for every 2 samples for background noise. Simply follow the procedure
tested. without adding any of the ATP-containing sample
into the analysis and record this value as RLU bg .
**** Note that Quench-Gone Organic filters are optimized for
Typical RLU bg values when using a PhotonMaster
compatibility with all organic fluids, including biofuels. These

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Test Kit Instructions – Quench-Gone Organic Modified

or Lumitester C-110 are ≤ 10. If high RLU bg are Step 1 – Calibration

consistently observed, repeat assays in an area
out of direct sunlight or intense lighting. A single The ATP Standard Calibration (ATP1) converts
RLU bg may be used for multiple analyses much luminometer RLU values into actual ATP
like a single UltraCheck 1 RLU (RLU ATP1 ). concentrations. Perform one calibration per day or for
each set of samples analyzed at the same time. Be
Handling Luminase sure that all reagents (especially rehydrated
Luminase) are allowed to reach ambient temperature
• Luminase is manufactured using a process called prior to use.
freeze-drying. This maximizes product stability
PROCEDURE: Holding the bottle vertical, add 2 drops
prior to use. Before using this product, it must first
(100uL) of UltraCheck 1 to a new 12x55mm test tube
be rehydrated by mixing freeze-dried pellet with
(the Assay Tube). Use a new pipet tip to dispense
liquid buffer and then allowed to incubate for at
100µL of Luminase, swirl gently five times,
least 5 minutes. Take care to avoid contamination
immediately insert into the luminometer and measure.
when removing the glass vial stopper.
Record RLU ATP1 .

Luminase Rehydration Process

NOTE: When using the QGOM-XLPD method, substitute
• Rehydrated Luminase can be stored in the Luminase with LuminaseXL.
refrigerator for up to 3 months (or freezer for up to NOTE: If RLU ATP1 ≤ 5,000 using a PhotonMaster or
6 months with unlimited freeze-thaw cycles) Lumitester C-110 rehydrate a new bottle of Luminase for
following rehydration. Always bring cold maximum sensitivity.
rehydrated Luminase to ambient temperature prior NOTE: RLU ATP1 will fall over time for the same batch of
to use, because reaction kinetics will change with Luminase. This is due to decreased luciferase enzyme
temperature. 1 hour is generally sufficient for this activity. When followed, the guideline above ensures that
purpose. there is sufficient activity to meet the specified detection limit.

• Never expose rehydrated Luminase to ≥30oC for

longer than 1-2 hours.
Step 2 – QGO-M cATPTM Analysis
• In general, it is recommended that Luminase only The QGO-M Cellular ATP (cATP) analysis measures
be rehydrated as required. In other words, ATP from living cells only. Perform one cATP analysis
rehydrate on the day of testing rather than in on each sample you wish to test.
advance.
2.1 – MEASURE SAMPLE VOLUME
• Never attempt to partition portions of freeze-dried
Luminase enzyme and/or the supplied buffer into The QGO-M method allows flexibility in the amount of
smaller quantities. sample used for analysis. Use the following table to
select the quantity of sample to be filtered for your
• If you begin utilizing a new bottle of Luminase application. As a general rule of thumb, the greater
during your testing, make sure to collect a new your sample volume, the greater the sensitivity you will
calibration result for that bottle. Alternatively, mix be able to achieve!
multiple bottles of Luminase for all testing at one
time.

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 Test Kit Instructions – Quench-Gone Organic Modified

QGO-M Sample Volume Recommendations 2.2 – SAMPLE FILTRATION

Recommended
Sample Type Slowly push the entire sample volume through the filter
Volume (mL)
and into a plastic waste receptacle at a rate of
Polymers, Admixtures, Personal or
1 (Diluted) * approximately 3-5mL per second. Push the plunger far
Home Care Products
Metalworking Fluids, Fuel enough to filter the sample and stop to ensure that the
1 to 5
Associated Water, Crude Oil filter remains wet. Detach the filter and remove the
Finished Fuel, Lubricants ** 10 to 20 plunger.

Oilfield, Oily Brines 10 to 20

* High solids & complex fluids should be pre-diluted prior to

analysis using the QGOM-XLPD method. This ensures
reliable filtration and recovery of cATP. The recommended
dilution is 1mL sample in a 9mL UltraLute (Dilution) Tube and
subsequent filtration of the entire pre-diluted volume. If
NOTE: If the full volume of sample could not be filtered,
difficulty is encountered pipetting 1mL of the sample, acquire
record the actual volume processed.
wide-bore pipet tips or increase the bore size of supplied tips
using a clean pair of scissors. Refer to the following graphic NOTE: Extremely dry organic fluids may tend to ‘spray’ out of
on pre-dilution instructions. the filter nozzle. If this is observed, reduce the rate of
filtration.
NOTE: Use plastic beakers for waste vessels, not glass
ones. Glass can have an affinity for polar fluids.
NOTE: If you encounter difficulty achieving complete filtration
of your fluid sample, consider using the pre-dilution method
mentioned above or changing to a test kit more suitable for
** When testing finished fuels and lubricants, it is highly the sample type. Contact LuminUltra for recommendations.
recommended that aqueous and organic phases be analyzed
TIP: If increased sensitivity is desired, filter additional sample
separately so as to ensure reliable interpretation. When
by repeating 2.2 and 2.3 using the same syringe and filter.
testing fuels and lubricants, ensure that they meet water
content standards (from ASTM, ISO, or otherwise) prior to
proceeding with the analysis. Diesel fuels should contain ≤ 2.3 – FILTER WASHING
500 ppm water and sediment (per ASTM D 975 and D 7467),
Re-attach the filter to the 20mL syringe barrel and use
while jet fuels should contain ≤ 30 ppm (per ASTM D 1655).
a disposable bulb pipet or micropipettor to add 5mL of
Mix your sample to ensure homogeneity. Remove the LumiClean to the syringe barrel. Pass the LumiClean
plunger from a 20mL syringe and attach a filter. Pour slowly through the filter to dryness and collect into the
the appropriate volume of sample into the barrel of the waste receptacle.
syringe.

TIP: The same 5mL bulb pipet can be used to dispense

TIP: Alternatively, the syringe tip can be immersed into the multiple aliquots of LumiClean at the same time, assuming it
sample and drawn into the barrel before attaching the filter, is kept clean (e.g. stored in its wrapper) between uses.
rather than pouring the sample into the barrel after attaching
the filter. Be sure the syringe tip is clean if this mechanism is 2.4 – FILTER DRYING
used.
Detach the filter from the 20mL syringe barrel, and
attach the filter to a 60mL syringe. While holding the
filter tip over a waste receptacle, push the plunger
slowly through the barrel to dry the filter.

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 Test Kit Instructions – Quench-Gone Organic Modified

ATP/mL) = 0 in calculations, or select a larger volume in Step

2.1 and repeat the analysis.
NOTE: When RLU cATP ≤ 50 on a PhotonMaster or Lumitester
C-110, it is recommended that you measure and subtract
RLU bg from your measurement. When possible, repeat the
test procedure with a larger volume of sample to achieve a
TIP: The same 60mL syringe can be used to dry many filters. higher RLU cATP and greater accuracy.
As a general rule, replace the 60mL syringe for every 20
TIP: If “Scale Over” is returned, repeat the analysis using a
tests performed, so long as the syringe is stored in a clean,
dust-free location in its wrapper when not in use. smaller sample volume in 2.1.

2.5 – EXTRACTION 2.7 – CALCULATIONS

Re-attach the filter to the 20mL syringe barrel. Use the Following completion of QGO-M analyses, RLU values
micropipettor to add 1mL of UltraLyse 7 to the barrel. must be converted to ATP concentrations using the
Pass the UltraLyse 7 slowly through the filter to following calculations. For easy calculations, utilize
dryness and collect in a new 9mL UltraLute (Dilution) LuminUltra Cloud.
Tube. Cap and invert three times to mix. Cellular ATP (cATP) represents the amount of ATP
contained within living cells and is a direct indication of
total living biomass quantity.
RLU cATP 10,000 ( pg ATP )
cATP ( pg ATP / mL ) = ×
RLU ATP1 V Sample (mL )

NOTE: For pre-diluted samples, V sample should be the actual

NOTE: When using the bulk-format version, you must quantity of sample that was filtered. Normally, this will be
dispense your own reagent into a clean test tube. 1mL; however, if only a portion of the diluted sample could be
filtered, V sample should be the volume of initial sample
NOTE: At this point, the contents of the Dilution Tube are
multiplied by the ratio of the actual diluted sample volume
stable at room temperature for up to 4 hours.
filtered and the initial diluted sample volume.
TIP: For longer stability, collect the 1mL extract in a
NOTE: When applicable, subtract RLU bg from RLU cATP prior
separate, empty tube and store between 2-8oC for up to 1
to executing the above calculation.
week prior to dilution.
To communicate results on the same basis as
2.6 – ASSAY traditional culture tests, cATP results are converted
into Microbial Equivalents (ME’s). This is based on the
Using a new pipet tip, transfer 100µL from the
established conversion that 1 E. coli-sized bacteria
UltraLute (Dilution) Tube to a new 12x55mm test
contains 0.001 pg (1 fg) of ATP.
tube (the Assay Tube), and use another new pipet tip
to add 100µL of Luminase, swirl gently five times, 1 ME
cATP (ME / mL ) = cATP ( pg ATP / mL ) ×
immediately insert into the luminometer and measure. 0.001 pg ATP
Record RLU cATP .
NOTE: For more discussion on the quantity of ATP per cell,
visit www.luminultra.com.

Because many traditional culture-based methods

report results in a similar fashion, it is sometimes
convenient to report cATP results in ME/mL using
Scientific Notation (i.e. #.# x 10#) or on a Log 10 format
NOTE: When using the QGOM-XLPD method, substitute for comparison purposes.
Luminase with LuminaseXL.
NOTE: If RLU cATP ≤ 10 on a PhotonMaster or Lumitester C-
110, you are below the low–detection limit. Report cATP (pg

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Test Kit Instructions – Quench-Gone Organic Modified

QGO-M cATP Interpretation Guidelines

Interpretation Guidelines

(pg cATP/mL)

(pg cATP/mL)

(pg cATP/mL)
Good Control

Preventive
Once QGO-M cATP results are calculated, microbial

Corrective
Action

Action
control can be evaluated. ATP-based measurements Application
are extremely sensitive to changes in total microbial
quantity. In general, processes will have the best
microbial control when cATP is minimized. For the
Finished Fuels, Conventional 10 to
easiest interpretation, utilize LuminUltra Cloud. Lubricants*
<10
100
>100

LuminUltra’s ATP test kits can be used to audit Polymers, Admixtures, Personal 100 to
<100 >1000
microbial quantity to reveal differences at different Care, Home Care 1000
process locations in an effort to quickly assess the ‘hot Crude Oil, Fuel Associated
100 to
Water, Oily Brines, Chemical <100 >1,000
spots’ within a process that require more immediate 1,000
Products, Oilfield Waters**
attention. Metalworking Fluids, Fire- 1,000 to
<1,000 >10,000
Retardant Lubricants 10,000
For process control, daily monitoring using ATP test
kits will give you true total microbial quantity * The interpretation guidelines provided above are based on
parameters to trend over time against process samples that meet defined standards (from ASTM, ISO, or
characteristics and performance. otherwise) for water content. For example, diesel fuels
should contain ≤ 500 ppm water and sediment (per ASTM D
When utilizing ATP test kits it is important to remember 975 and D 7467), while jet fuels should contain ≤ 30 ppm
that every process is different. During audits, relative (per ASTM D 1655). If the sample has greater water content
comparisons from point to point are a reliable means to than defined as acceptable by the standard, interpretation
assess your process, while for daily monitoring it is guidelines may vary. Contact LuminUltra for interpretation
important to establish a baseline trend before making advice.
control decisions. To get started, LuminUltra provides ** Oxidizing biocides include chlorine, bromine, ozone, and
the following guidelines in units of pg cATP per mL: others. Given their different mechanism of kill, non-oxidizing
biocides and non-chemical treatments will typically have a
higher residual cATP when processes are well controlled.
Allow at least 1 hour to assess the true efficacy of an
oxidizing biocide and 24 hours for non-oxidizers.
NOTE: These interpretation guidelines are designed for
generic risk management guidance only. Users are
encouraged to establish their own control ranges on which to
base process decisions. LuminUltra and its affiliates do not
accept any liability for any decision or assessment taken or
made as a consequence of using this test kit.

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Test Kit Instructions – Quench-Gone Organic Modified

Ordering Information
• To obtain pricing information, inquire about other
• New to 2nd Generation ATP technology? Start by
products and services, or to place an order,
ordering the PhotonMaster & PBM Equipment Set
contact LuminUltra or your authorized
(Product # EQP-PBM-PAC) and the test kit(s) of
representative.
your choice.
• When reordering materials for testing, it is LuminUltra Technologies Ltd.
preferred to order complete kits. QGO-M is 520 King Street
available in seven formats: Fredericton, New Brunswick
Canada E3B 6G3
Description Part # Tel: +1-506-459-8777
QGO-M, 100 Tests, Complete * QGOM-100C Fax: +1-506-453-9860
QGO-M, 100 Tests, Reagents Only QGOM-100 www.luminultra.com
QGO-M, 100 Tests, Bulk Format ** QGOM-100B
QGOM XLPD, 100 Tests, Complete QGOM-XLPD- To order: [email protected]
*** 100C For all other inquiries: [email protected]
QGOM XLPD, 100 Tests, Reagents QGOM-XLPD-
Only *** 100
• Major credit cards (Visa, MasterCard, AMEX) are
QGO-M, 25 Tests, Complete * QGOM-25C
accepted. Contact LuminUltra by phone to place
QGO-M, 25 Tests, Reagents Only QGOM-25 credit card orders.
* Complete kits include LuminUltra-manufactured
reagents plus all consumables (tips, tubes, filters,
syringes) required to run analysis. If you supply your
own consumables, reagent only kits are available.
** Bulk test kits contain all reagents supplied in bulk • Orders generally ship within 3 business days. You
format and require the user to dispense individual will receive order confirmation via Email.
quantities as required.
Lumitester is a trademark of Kikkoman Corporation. All other
*** QGOM-XLPD contains LuminaseXL instead of trademarks are the property of LuminUltra Technologies Ltd.
Luminase and additional Dilution Tubes.

www.luminultra.com © LuminUltra 2017 Page 8 of 8

 Quick Reference Guide NOTE: Please refer to Test
Kit Instructions during first
Quench-GoneTM Organic Modified Test Kit product use and for
additional details including
Product #: QGOM-25 / QGOM-100 legal statements.

Step 1 - UltraCheckTM 1 Calibration

Perform one UltraCheck 1 calibration per day or per each set of samples analyzed.

NOTE: If RLUATP1 ≤ 5,000 using a PhotonMaster or Lumitester C-110, rehydrate a

new bottle of Luminase for maximum sensitivity.

Step 2 - Cellular ATP (cATPTM) Analysis Calculations

Total Cellular ATP (cATP) Calculation:
2.1 – SELECT SAMPLE VOLUME 2.2 – FILTRATION 2.5 EXTRACTION RLU cATP 10,000 ( pg ATP )
cATP ( pg ATP / mL ) = ×
Determine volume and filter sample. Filter sample. Extract ATP from filter & dilute. RLU ATP1 V Sample (mL )
Microbial Equivalent (ME/mL):
Volume
Sample Type 1 𝑀𝑀𝑀𝑀
(mL) 𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐 (𝑀𝑀𝑀𝑀 � 𝑚𝑚𝑚𝑚) = 𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐 (𝑝𝑝𝑝𝑝 𝐴𝐴𝐴𝐴𝐴𝐴�𝑚𝑚𝑚𝑚) ×
0.001 𝑝𝑝𝑝𝑝 𝐴𝐴𝐴𝐴𝐴𝐴
Polymers, Admixtures, Personal
1 (Diluted)* Interpretation Guidelines
or Home Care Products

Metalworking Fluids, Fuel

1 to 5

(pg cATP/mL)
Good Control
(pg cATP/mL)

(pg cATP/mL)
Preventative
Associated Water, Crude Oil

Corrective
Action

Action
Application
Finished Fuel, Lubricants** 10 to 20 2.3 – FILTER WASHING 2.6 – ASSAY
Wash filter of organic contaminants. Measure ATP concentration.
Oilfield, Oily Brines 10 to 20
Finished Fuels,
< 10 10 to 100 > 100
Conventional Lubricants

Polymers, Admixtures, 100 to

< 100 > 1,000
Personal Care, Home Care 1,000

Crude Oil, Fuel Associated

Water, Oily Brines, 100 to
< 100 > 1,000
*See note in Test Kit Instructions for sample pre-dilution NOTE: If RLUcATP ≤ 10 using a PhotonMaster or Chemical Products, 1,000
recommendations. Lumitester C-110, you are below the low- Oilfield Waters
2.4 FILTER DRYING detection limit.
**When testing finished fuels and lubricants, it is highly Metalworking Fluids, Fire- 1,000 to
recommended that aqueous and organic phases be Dry filter to remove volatile organics. Retardant Lubricants
< 1,000
10,000
> 10,000
NOTE: If RLUcATP ≤ 50 using a PhotonMaster or
analyzed separately so as to ensure reliable interpretation. Lumitester C-110, consider accounting for
When testing fuels and lubricants, ensure that they meet background (RLUbg ). See Test Kit Instructions for
water content standards (from ASTM, ISO, or otherwise) guidance. NOTE: Interpretation Guidelines provided for general guidance.
prior to proceeding with the analysis. Diesel fuels should For best results, establish your own baseline and control levels.
contain < 500 ppm water and sediment (per ASTM D 975
and D 7467), while jet fuels should contain < 30 ppm water
(per ASTM D 1655)

Please refer to Test Kit Instructions for calculations and

Date Modified: 13-Dec-16 © LuminUltra 2017 interpretation advice, or use LuminUltra Cloud.