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    Role of HPTLC in Characterization of Herbal Medicines PDF

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    Role of HPTLC in Characterization of Herbal Medicines PDF

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    Role of HPTLC in Characterization of Herbal Medicines PDF

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    Anchrom HPTLC

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    Modern Analytical Tools used for Characterization of Herbal Medicines

    Market scenario of medicinal plants

    The market for medicinal plants in India stood at Rs. 4.2 billion (US$ 56.6 million) in 2019
    and is expected to increase at a CAGR of 38.5% to Rs. 14 billion (US$ 188.6 million) by
    2026.
    The total world herbal trade is currently assessed at US$ 120 billion. India’s share in the
    global export of herbs and herbal products is low due to unsophisticated agricultural and
    quality control procedures, lack of processing, research & development, standardization in
    products and regulatory framework in the trade of medicinal plants.

    To address this problem it is necessary to standardize medicinal plants and formulations


    prepared from herbal ingredients by using modern analytical techniques e.g.
    Chromatography and spectroscopic techniques.

    What are the challenges in characterizing herbal medicines?

    Herbal medications are difficult to characterize because they are complex mixtures of
    numerous bioactive compounds that vary greatly in composition, quantity, and biological
    activity. Plant species, geographical origin, cultivation methods, harvest timing, storage
    conditions, and processing procedures all contribute to this heterogeneity.

    The introduction of substitutes or the unintentional/intentional addition of confounded


    materials complicates the characterization of herbal medicines.

    Mass spectrometry (MS), Nuclear magnetic resonance spectroscopy (NMR), gas


    chromatography (GC), Fourier transform infrared (FTIR) spectroscopy, Ultra-performance
    liquid chromatography (UPLC), High-performance liquid chromatography (HPLC),
    High-Performance thin layer chromatography (HPTLC), and such chromatographic and
    spectroscopic techniques are used for the characterization of herbal medicines. Of these
    techniques, HPTLC would be the ideal alternative to standardize many characteristics of
    herbal medicines since it is an easy, versatile, sensitive, cost-effective, and non-destructive
    technology that enables high-throughput study of herbal medicines. Its capacity to separate
    and identify a broad spectrum of compounds, even those present in low concentrations,
    makes it a powerful tool for herbal medicine characterization.

    Role Of HPTLC In Characterization Of Herbal Medicines

    High Performance Thin Layer Chromatography (HPTLC) is one of the best chromatographic
    techniques for herbal analysis.

    ​ It is the only chromatographic technique in which we can record the results in the
    form of images/pictures.
    ​ HPTLC is a type of planar chromatography technique and it is a GLP/USP/EP
    compliant chromatographic technique.
    ​ HPTLC is a simple, flexible, cost-effective and fast technique. More than 100 samples
    can be analyzed in a day and report can be generated in approximately 60-90 minutes.
    ​ HPTLC has applications in numerous fields e.g. herbal, food, pharma, cosmetic,
    forensic etc. However, different categories of herbal samples e.g. AYUSH samples,
    Nutraceuticals, dietary supplements can also be analyzed.
    ​ HPTLC has tremendous applications in AYUSH labs, herbal research labs and
    manufacturing units, academic institutes etc.

    What type of characterization studies can be done by HPTLC?

    1. Fingerprint studies are conducted for herbal raw materials or finished product to establish
    the identity of the said material.

    2. The correlation between raw material and finished product can be established by an
    identification test by HPTLC.
    3. Stability studies can also be performed by HPTLC using two approaches: fingerprint
    studies or quantification of phytomarkers present in the formulation.

    4. Quantification of respective phytomarkers and method validation studies can also be


    performed in herbal raw material and finished product for quality testing or optimization
    procedures of related herbal samples.

    5. HPTLC can also be coupled with a mass analyzer for the identification of separated
    analytes. USP Chapter 2251 states the use of the TLC-MS interface for the identification of
    synthetic API in herbal formulations.

    6. HPTLC can also be coupled with bioactivity studies, e.g., antioxidant, antidiabetic,
    antimicrobial, etc. These types of studies help with fast screening for a large number of
    samples in less time and give information about the therapeutic activity of the sample.

    Let us see one example in which you can understand the simultaneous
    identification and quantification of four markers in Withania somnifera
    aerial and root parts by HPTLC:

    Ashwagandha or Withania somnifera also known as ‘Indian ginseng’ is an herb used to calm
    the brain, reduce swelling, lower blood pressure, treat insomnia, boost nutrition and alter the
    immune system.

    We, at Anchrom have developed a simultaneous method for identification and quantification
    of Withanoside – IV, Withanoside – V, Withaferin A and Kaempferol-3-7-di-O-glucoside
    from Withania somnifera aerial parts and root part.
    A combination standard mixture was prepared and along with it the samples of aerial parts
    and root part were prepared and applied on the plate. After development, four respective
    standards were detected after derivatization with anisaldehyde sulphuric acid in white light.

    Following table shows the data for quantitative analysis performed for the samples (aerial
    parts & root part) provided by the customer.

    Sample Kaempferol-3-7-di-O-glu Withanoside – Withanoside – Withaferin


    coside IV V A

    Aerial Parts 0.21% – – 0.43%


    extract

    Root part – 0.146% 0.153% 0.08%


    extract

    Anchrom Enterprises Pvt. Ltd is one of the leaders in HPTLC analysis. Please contact us at
    [email protected] for HPTLC analysis of plant extracts, drugs, ingredients in cosmetics, and
    forensic science.

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