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    Questions Rna Extraction Sample and Purify Mrna From Pigs

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    Ivory Mae Verona Andaya
    This document contains a series of multiple choice questions about extracting RNA from pig tissue samples. Specifically, it covers topics like what mRNA is, why tissues are stored in liquid nitrogen, the components of cell RNA content and lysates, measuring RNA purity and quality using a spectrophotometer, and visualizing intact RNA on a gel. The overall goal appears to be testing understanding of the RNA extraction process from biological samples.

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    Questions Rna Extraction Sample and Purify Mrna From Pigs

    Uploaded by

    Ivory Mae Verona Andaya
    221 views2 pages
    This document contains a series of multiple choice questions about extracting RNA from pig tissue samples. Specifically, it covers topics like what mRNA is, why tissues are stored in liquid nitrogen, the components of cell RNA content and lysates, measuring RNA purity and quality using a spectrophotometer, and visualizing intact RNA on a gel. The overall goal appears to be testing understanding of the RNA extraction process from biological samples.

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    Questions-rna-extraction-sample-and-purify-mrna-from-pigs (1)

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    This document contains a series of multiple choice questions about extracting RNA from pig tissue samples. Specifically, it covers topics like what mRNA is, why tissues are stored in liquid nitrogen, the components of cell RNA content and lysates, measuring RNA purity and quality using a spectrophotometer, and visualizing intact RNA on a gel. The overall goal appears to be testing understanding of the RNA extraction process from biological samples.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    221 views2 pages

    Questions Rna Extraction Sample and Purify Mrna From Pigs

    Uploaded by

    Ivory Mae Verona Andaya
    This document contains a series of multiple choice questions about extracting RNA from pig tissue samples. Specifically, it covers topics like what mRNA is, why tissues are stored in liquid nitrogen, the components of cell RNA content and lysates, measuring RNA purity and quality using a spectrophotometer, and visualizing intact RNA on a gel. The overall goal appears to be testing understanding of the RNA extraction process from biological samples.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    RNA Extraction: Sample and purify mRNA from

    pigs
    Question 1: What word fits in the gap?

    When a gene is expressed ... is produced.


    ✓ mRNA
    – siRNA
    – a signal
    – a complementary DNA strand

    Question 2: All genes are expressed in all the cells. Is this statement true?
    ✓ No, only genes that are relevant for that particular cell are expressed
    – No, only half of them are expressed in all the cells
    – No, all genes are only expressed in the young cells and then the expression level decreases
    – That's true

    Question 3: If mRNAM R N Ay from gene A is very abundant in the fat tissue from the obese pig
    and rare in the fat tissue from the lean pig it shows us that .... ?
    ✓ Gene A is more expressed in obese animals
    – No conclusion can be drawn from this data
    – Gene expression is random
    – Gene A is less expressed in obese animals

    Question 4: Why do we keep tissue samples for RNA work in liquid nitrogen (-196oC)?
    ✓ To preserve the RNA, which easily degrades at room temperature
    – To deactivate the RNA
    – To preserve the tissue structure
    – To keep the fat tissue from melting

    Question 5: The RNA content in a cell ....


    ✓ Includes both coding mRNA and several types of non-coding RNA
    – Includes only coding mRNA
    – Includes only mRNA and tRNA
    – Is restricted to mRNA

    Question 6: Before we start, do you know where is most of the mRNAM R N Ay located?
    ✓ In the cytoplasm
    – In the nucleus
    – In the cellular membrane
    – In the mitochondria

    Question 7: The lysis buffer used for RNA extraction contains ...
    ✓ Guanidinium isothiocyanate and phenol
    – None of these answers
    – Isopropanol
    – Chloroform

    Question 8: What does the lysate consist of?


    ✓ RNA, DNA, proteins and cellular debris
    – Intact cells
    – RNA and proteins

    Page 1
    – RNA, DNA, proteins

    Question 9: What do you measure at 230, 260 and 280 nm, respectively?
    ✓ Chemicals, RNA/DNA and proteins
    – RNA at all wavelength
    – Proteins, DNA and RNA
    – DNA, proteins and RNA

    Question 10: A 260/280260 to 280 ratio should be between 1.8 and 2.1. What does it mean if we
    have a 260/280260 to 280 ratio that is lower than 1.8?
    ✓ There are a lot of proteins in the RNA sample
    – The RNA sample is very clean
    – The RNA sample is very degraded
    – There are some chemical remnants e.g. phenol in the RNA sample

    Question 11: What will a spectrophotometer measure?


    ✓ Purity and quantity
    – Purity and integrity
    – Only purity
    – Purity, integrity and quantity

    Question 12: What should you see in the gel if the RNA is intact?
    ✓ Two bands corresponding to ribosomal RNAs (rRNAs)
    – A smear
    – One band corresponding to all mRNAs
    – Two bands corresponding to ribosomal RNas (rRNAs) and a third band corresponding to
    mRNAs

    Question 13: Why do we want to specifically extract the mRNAM R N Ay only from the total RNA?
    ✓ Because mRNA represents the genes that are expressed and will be translated into proteins
    – Because mRNA represents all the other types of RNA
    – Because mRNA is located only in the nucleus
    – Because mRNA is the most abundant RNA and hence it is easy to sequence

    Question 14: What is the common sequence that all mRNAM R N Ay share?
    ✓ polyA
    – polyG
    – polyT
    – polyC

    Page 2

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