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Group 1 (Ultracentrifugation)

The document discusses techniques for separating analyte mixtures including ultracentrifugation and chemiluminescence. Ultracentrifugation uses very high rotational speeds to separate particles of different sizes and densities. It can provide information about macromolecular properties in solution. Chemiluminescence is a chemical reaction that produces light and is often used in clinical and forensic applications to detect substances like hormones, proteins, DNA, and blood.

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Jazyl Tan
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0% found this document useful (0 votes)
86 views9 pages

Group 1 (Ultracentrifugation)

The document discusses techniques for separating analyte mixtures including ultracentrifugation and chemiluminescence. Ultracentrifugation uses very high rotational speeds to separate particles of different sizes and densities. It can provide information about macromolecular properties in solution. Chemiluminescence is a chemical reaction that produces light and is often used in clinical and forensic applications to detect substances like hormones, proteins, DNA, and blood.

Uploaded by

Jazyl Tan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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GROUP 1 (ULTRACENTRIFUGATION)

1. This refers to a technique for separating an analyte mixture based on the centrifugal
force created by a very fast rotation, typically 50,000 rpm to 150,000 rpm.
a. Analytical Ultracentrifugation
b. Ultracentrifugation
c. Preparative ultracentrifugation
d. Relative centrifugal force
2. A method for investigating a wide range of macromolecular properties, such as size,
shape, stoichiometry, and binding properties, all in the actual solution-state environment,
which other orthogonal techniques lack.
a. Analytical Ultracentrifugation
b. Ultracentrifugation
c. Preparative Ultracentrifugation
d. Relative centrifugal force
3. What type of rotors that contain sample tube openings at a fixed angle from the central
axis.
a. Zonal rotors
b. Swinging bucket rotors
c. Fixed-angle rotors
d. Rotor balance
4. The experimental observation and analytical interpretation of the temporal evolution of
dissolved particle spatial distributions in a centrifugal field.
a. Rotors
b. Sedimentation equilibrium
c. Ultracentrifugation
d. Sedimentation velocity
5. The observation and interpretation of solute particle equilibrium distributions in a
centrifugal field.
a. Rotors
b. Sedimentation equilibrium
c. Ultracentrifugation
d. Sedimentation velocity
6. It consists of a ring of individual tube holders mounted on hinges that allow the tubes to
swing outward when the spinning begins.
a. Zonal rotors
b. Swinging bucket rotors
c. Fixed-angle rotors
d. Rotor balance
7. Instead of multiple sample tubes, which are designed to hold one sample of a larger
volume?
a. Zonal rotors
b. Swinging bucket rotors
c. Fixed-angle rotors
d. Rotor balance
8. Who is the colloid chemist who developed the first ultracentrifuge as an analytical tool?
a. Antonin Prandtl
b. Theodor Svedberg
c. Friedrich Miescher
d. Edward Greydon Pickels
9. Frequently used for separating particles based on density, isolating and/or harvesting
denser particles for collection in the pellet, and clarifying particle-containing
suspensions.
a. Analytical Ultracentrifugation
b. Ultracentrifugation
c. Preparative Ultracentrifugation
d. Relative centrifugal force
10. It is determined by two factors: the rotor radius and the rotational speed squared.
Different applications may necessitate different amounts of force.
a. Analytical Ultracentrifugation
b. Ultracentrifugation
c. Preparative Ultracentrifugation
d. Relative centrifugal force

GROUP 2 (CHEMILUMINISCENCE)
1. What substance did Henning Brand find in human urine?
a. Gold
b. Silver
c. Copper
d. Phosphorus

2. Chemiluminescence is often referred to as “cold light.” In chemiluminescence, an atom’s


electron is released from its highest energy excited state into a lower energy ground state.
a. Both are true
b. Both are false.
c. First Statement is True. Second statement is false.
d. First statement is false. Second statement is true.

3. Which is true about chemiluminescence?


1. The transition site is where enthalpy is at its maximum.
2. The chemical reaction occurs because it decreases the stability
3. The chemical reaction occurs because it decreases the energy of the molecules.
4. When chemicals are combined, chemiluminescence takes place and produces light as a
byproduct.
a. 1, 2, 3, 4
b. 2, 3, 4
c. 1, 2, 4
d. 1, 3, 4
4. What is the relationship between energy and stability in a chemical reaction
(chemiluminescence)?
a. As energy decreases, stability increases
b. As energy decreases, stability decreases
c. As energy increases, stability increases
d. There is no relationship between energy and stability.

5. What is the role of energy in chemiluminescence?


a. It is used to stimulate the chemical molecule that generates light
b. It is produced by the chemical reaction that generates light.
c. It is used to break apart the chemical molecules.
d. It is used to create a new chemical compound.

6. How long does it take to measure light in flash-type reactions in chemiluminescence?


a. 10 min
b. 10 sec
c. 5 sec
d. 5 min

7. What is the main disadvantage of chemiluminescence?


a. High cost
b. Slow speed
c. Complicated instrumentation
d. Impurities causing background signal degradation

8. What is the pharmaceutical industry using chemiluminescence technology for?


a. To inspect drugs for impurities
b. To screen biological compounds for contamination.
c. To treat diseases
d. Both a and b

9. What can be measured using chemiluminescence in clinical laboratories?


a. Only DNA
b. Hormones, proteins, and peptides
c. Only proteins
d. None of the above.

10. In what ways is chemiluminescence commonly used in forensic medicine?


a. To detect fingerprints
b. To detect the presence of blood
c. To analyze hair samples through light
d. To analyze DNA

GROUP 3 (ELECTROCHEMISTRY)
1. The study of the correlation between electrical energy and chemical changes is the focus of
the branch of chemistry known as electrochemistry.
A. Electron Energy
B. Electrochemistry
C. Chemistry
D. Physics

2. This is a chemical process in which chemical entities transmit electrons to one another.
A. Chemical Reaction
B. Electron Reaction
C. Redox Reaction
D. Physical Reaction

3. Electrochemical reactions are those in which electric currents are either generated or input.
These responses can be roughly divided into two categories. What are the correct phrases for
these two categories?
A. Production of chemical energy into electrical energy and Conversion of chemical change
by electrical energy.
B. Production of chemical energy into chemical change and Conversion of chemical
change by electrical energy.
C. Production of chemical change by electrical energy and Conversion of chemical energy
into electrical energy.
D. Production of chemical change by chemical change and Conversion of electrical energy
into electrical energy.

4. He is the father of modern electrochemistry and a chemist who co-discovered


electrochemiluminescence and made substantial contributions to the development of scanning
electrochemical microscopy.
A. Alessandro Volta
B. Allen J. Bard
C. Luigi Galvani
D. Michael Faraday

5. Based on the main branches of Electrochemistry


I.Chemical reactions that create electricity such as batteries
II. Chemical reactions that use electricity to produce new compounds

A. First statement is wrong and Second statement is correct


B. First statement is correct and Second statement is wrong
C. Both Correct statement
D. Both Wrong statement

6. This cell type is capable of generating chemical energy from chemical processes utilizing
electrical energy and chemical energy from electrical energy.
A. Electrochemical Cell
B. Galvanic cell
C. Voltaic cell
D. Electrolytics cell
7. Answer if the question is true or false.
The Oxidation keyword is the loss of electrons while the Reduction is the gain of electrons.
A. True
B. False
C. Maybe
D. Partly true

8. What are the two types of electrochemical cells?


A. Galvanic cell and Voltaic cell
B. Galvanic cell and electrolytic cell
C. Electron cell and proton cell
D. None of the above

9. Identify if statements are true or false.


I. CATHODIC REACTION is an electrochemical cell processes that take place at the anode
II. ANODIC REACTION is the reducing processes that take place at the cathode
A. First statement is wrong and Second statement is correct
B. First statement is correct and Second statement is wrong
C. Both statement is Correct
D. Both statement is wrong

10. What are the electrode of REDUCTION: _________ and OXIDATION:_________


A. Anode and Cathode
B. Cathode and Anode
C. Anodic reaction and Cathodic reaction
D. Cathodic reaction and Anodic reaction

GROUP 4 (IMMUNICHEMISTRY)
1. Separate precipitin lines occur in ________ hours.
a. 10-15
b. 18-24
c. 18-22
d. 10-18

2. The following are the results of immunoelectrophoresis, EXCEPT?


a. No reaction if formed precipitate do not exist
b. Presence of elliptical or shaped-like ellipse principate
c. Presence of two individual serum proteins
d. Different antigens (proteins) can be identified

3. Who develop the first smallpox vaccination and the variolation hypothesis that provided
evidence that immune responses might be controlled and that the body's own defense
mechanisms were crucial for sustaining healthy biological structures?
A. Paul Ehrlich
B. Edward Jenner
C. Emil von Behring
D. None of the above

4. In what year did the German scientist Emil von Behring found that serum antibodies might be
used to treat diphtheria and tetanus?
A. 1975
B. 20th century
C. 1790
D. 1890

5. It is an immunochemical method for precipitating a protein antigen from a solution by using


an antibody that binds to the desired protein alone.
a. Enzyme-Linked Immunosorent Assay (ELISA)
b. Immunoelectrophoresis
c. Immunohistochemistry
d. Immunoprecipitation

6. This classification of immunoassay is used to measure the analyte bound site.


a. Competitive Immunoassay
b. Non-competitive Immunoassay
c. Homogeneous Immunoassay
d. Heterogeneous Immunoassay

7. Also known as Immunoglobulin


a. Antibody
b. Hemoglobin
c. Antigen
d. Vitamin C
8. It tells your immune system whether something in your body is harmful or not.
a. Antigen
b. Adipose tissue
c. Antibody
d. Serum

9. The simple colorimetric lateral flow assay, which is used for ______________ and the
detection of a wide range of human and veterinary diseases, as well as food safety and
environmental testing, is one of the most well-known types of immunoassay.
a. HIV testing
b. Newborn screening
c. Pregnancy test
d. Water analysis

10. It is a subfield of chemistry that investigates the elements and operations of the immune
system, particularly the characteristics and relationships between antigen and antibody.
a. Immunocytochemistry
b. Immunohistochemistry
c. Organic chemistry
d. Immunochemistry

GROUP 5 (WET CHEMISTRY)


1. Methods in Wet chemistry which the endpoint is identified through precise measurement.
A. Classic Analysis

B. Gravimetric Analysis

C. Colorimetry

D. Volumetric Analysis

2. Measurement, mixing, and weighing can all be done using wet chemistry. Reagents are
employed in chemical testing to reveal the existence of a particular chemical in a test solution
A. First Statement is True. Second statement is False

B. First Statement is False. Second statement is True

C. Both Statement is True

D. Both Statement is False

3. Wet chemistry typically uses ___________ like beakers and graduated cylinders to keep
materials from being contaminated or interfered with by unintended sources. Additionally,
gasoline, Bunsen burners, and crucibles may be used to evaporate and isolate substances in
their dry forms.
A. Laboratory glasswares

B. Containers

C. Sterile Containers

D. Glass Containers

4. Wet chemistry approaches that rely on analytical instruments rather than manual methods are
referred to as:
A. Classic Chemistry

B. Classical Wet Chemistry

C. Hard Chemistry
D. Instrumental Wet Chemistry

5. A word that describes chemistry that is typically carried out in the liquid phase. it involves the
use of laboratory glassware like beakers and flasks and excludes the use of instruments for
quantitative chemical analysis.
A. Immunochemistry

B. Electrochemistry

C.Wet Chemistry

D. Ultracentrifugation

6. Wet Chemistry is also known as classic chemistry or classical chemistry. The presence of
certain compounds can be ascertained by a technician by observing any unusual interactions
between the solution only.
A. First Statement is True. Second statement is False

B. First Statement is False. Second statement is True

C. Both Statement is True

D. Both Statement is False

7. A process frequently used when a component has a low solvent solubility.


A. Soxhlit Extraction

B. Sochlet Extraction C. Soxlet Extraction


D. Soxhlet Extraction

8. Which of the following is odd:


A. Gasoline

B. Beakers

C. Bunsen Burner D. Crucibles

9. If accuracy or efficiency are priorities, analytical tools may take the role ofmanual processes.
When extreme precision is desired, instrumental wet chemistry is frequently selected.
A. First Statement is True. Second statement is False
B. Fist Statement is False. Second statement is True

C. Both Statement is True

D. Both Statement is False

10. The popular wet chemistry method that relies on a color change as a result of a reaction.
A. Volumetric Analysis

B. Titration

C. Colorimetry

D. Gravimetric Analysis

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