REVIEWER FOR IQC MIDTERM EXAM

1. It is useful for isopycnic and in some cases, rate zonal separations when run time reduction is important.
a. fixed angle rotor b. vertical rotor c. swinging bucket rotor d. all of the choices
2. All of the following are limitations of chemiluminescence, except
a. low intensity light emission c. ultra-sensitive
b. light leaks d. high intensity light emission
3. During the electrolysis of an electrolyte, the number of ions produced is directly proportional to
a. time consumed c. quantity of electricity passed
b. electrochemical equivalent of electrolysis d. mass of electrons
4. It is the most important factor that affects the cumulative strength of bonding between the antigen and antibody.
a. Ka b. hapten c. epitope d. goodness/closeness
5. It is a technique used to determine the concentration of a solution by reacting it with a known volume and
concentration of another solution.
a. chromatography c. distillation
b. gravimetric analysis d. titration
6. What is sample purity used in analytical centrifugation?
a. pure b. relatively pure c. less pure d. clear
7. It is used to locate traces of blood that are not detectable with the naked eye.
a. NADPH b. luminol reactions c. aluminum oxide d. chemiluminometric assays
8. It is the measurement of the difference of half cells which are reduction potential and oxidation potential.
a. electrolysis b. voltaic cell c. cell potential d. electrode
9. Structurally, antibodies are often visualized as Y-shaped molecules, each containing ______ polypeptides.
a. 7 b. 10 c. 4 d. 2
10. Wet chemistry is used for elemental analysis of samples, except
a. chloride b. cyanide c. phenols d. none of the choices
11. This type of ultracentrifugation is based on the density differences of particles measured.
a. differential ultracentrifugation c. preparative ultracentrifugation
b. density gradient ultracentrifugation d. analytic ultracentrifugation
12. In chemiluminescence, the chemical compound that produces the light is __________ by the energy created in the
chemical reaction.
a. contracted b. excited c. processed d. created
13. These refer to substances that do not allow electricity to pass through.
a. insulator b. rubber c. metal d. conductor
14. It activates the complement proteins after antibodies bind to antigens and neutralize foreign invaders.
a. antibodies b. antigen c. immune complexes d. none of the choices
15. Which of the following statements is TRUE about systematic errors.
a. These refers to similarities in variation techniques.
b. These are errors that can easily be pinpointed.
c. These are errors that have inaccurate results.
d. These are errors that are not related to calibration problems.
16. It is designed for gradient centrifugation when there are components in a sample mixture that do not participate in
the gradient.
a. swing bucket rotor c. fixed angle rotor
b. vertical rotor d. near vertical rotor
17. The _________ of a centrifuge is measured in revolutions per minute or rpm.
a. temperature b. speed c. time d. minute
18. Which of the following is an example of chemiluminescence?
a. glow stick b. lamps c. headlights d. electric eels
19. In electrochemistry, what is a reducing agent?
a. It is the species that gets reduced—gained electrons.
b. It is the species that gets oxidized—loses electrons.
c. It is the species that creates electrons.
d. It is the species that destroys electrons.
20. In immunochemistry, antibodies are _________ that are produced by the ____________.
a. lipids, adipose cells c. nucleic acids, hepatocytes
b. proteins, B lymphocytes d. carbohydrates, bone marrow cells
21. In wet chemistry, the following are used to analyze elements and compounds in liquid samples, except
a. titrimetry b. colorimetry c. reflectivity d. gravimetry
22. What type of ultracentrifugation is frequently used for separating particles based on density, isolating and/or
harvesting denser particles for collection in the pellet, ad clarifying particle-containing suspension.
a. analytical ultracentrifugation c. preparative ultracentrifugation
 b. ultracentrifugation d. relative centrifugal force

23. In chemiluminescence, what is the relationship between energy and stability in a chemical reaction?
a. as energy decreases, stability increases c. as energy increases, stability increases
b. as energy decreases, stability decreases d. no relationship between energy and stability
24. How long does it take to measure light in flash-type reactions in chemiluminescence?
a. 10 min b. 10 sec c. 5 sec d. 5 min
25. What are the two types of electrochemical cells?
a. galvanic and voltaic cell c. electron and proton cell
b. galvanic and electrolytic cell d. none of the choices
26. It is an immunochemical method for precipitating a protein antigen from a solution by using an antibody that binds
to the desired protein alone.
a. enzyme-linked immunosorbent assay c. immunohistochemistry
b. immunoelectrophoresis d. immunoprecipitation
27. It is a popular wet chemistry method that relies on a color change as a result of a reaction.
a. volumetric analysis b. titration c. colorimetry d. gravimetry
28. What is the ultracentrifugation speed in rpm?
a. 200, 000 rpm b. 50, 000 rpm c. 300, 000 rpm d. 100, 000 rpm
29. In chemiluminescence, the intensity and color of light produced are not determined by the following, except
a. external light source c. color of the container
b. type of reactants d. none of the choices
30. These are extensively used for determining the pH of solutions, solubility product, equilibrium constant and other
thermodynamic properties.
a. litmus paper b. pH meter c. electrochemical cells d. cathode
31. In immunochemistry, this is a type of immunity that develops with exposure to various antigens.
a. innate immunity b. acquired immunity c. passive immunity d. immune response
32. This can be used to determine the size of a gravitational field or the properties of the matter that caused it to form.
a. Titrimetry b. gravimetry c. colorimetry d. none of the choices
33. All of the following are importance of electrochemistry, except
a. Used to produce different metals
b. Used for processing products of several chemicals
c. Used in the search for cell or tissue antigens
d. All of the choices
34. In immune precipitation methods, it is known as the simplest and least sensitive method.
a. Single diffusion technique c. double diffusion technique
b. Immunoelectrophoresis d. rocket technique
35. What is an electrolytic cell used for?
a. To store electrical energy
b. To convert potential chemical energy into electrical energy
c. To force a non-spontaneous chemical reaction to occur
d. To transfer electrons
36. All of the following are TRUE about ultracentrifuge, except
a. It is able to sediment colloidal and small particles
b. It is used in more facilitated laboratories that perform more advanced operations
c. It can spin as fast as 180, 000 rotations per minute.
d. It separates smaller molecules that cannot be separated from traditional centrifuges.
37. Chemiluminescence releases unstable compounds and produces more stable chemicals through
a. disintegrating b. heating c. interacting d. excitation
38. This law states that the amount of substance produced at each electrode is directly proportional to the quantity of
charge flowing through the cell.
a. Faraday’s Law b. Newton’s Law c. Kepler’s Law d. Boyle’s Law
39. Which of the following does not provide cryoprotection?
a. sucrose b. fructose c. glycerol d. DMSO
40. Which of the following is a measure that cannot be conducted using wet chemistry?
a. length b. temperature c. conductivity d. time