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    Aacc 02-31

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    Елена Старинова
    This document describes a method for measuring the titratable acidity or acid content of nonfat dry milk. The method involves dissolving a sample in water, adding a phenolphthalein indicator, and titrating the solution with a standardized 0.1N sodium hydroxide solution until a faint pink color persists. The ml of sodium hydroxide required is then used to calculate the percentage of acid as lactic acid. The method uses common laboratory equipment and reagents.

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    Aacc 02-31

    Uploaded by

    Елена Старинова
    186 views1 page
    This document describes a method for measuring the titratable acidity or acid content of nonfat dry milk. The method involves dissolving a sample in water, adding a phenolphthalein indicator, and titrating the solution with a standardized 0.1N sodium hydroxide solution until a faint pink color persists. The ml of sodium hydroxide required is then used to calculate the percentage of acid as lactic acid. The method uses common laboratory equipment and reagents.

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    AACC 02-31

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    This document describes a method for measuring the titratable acidity or acid content of nonfat dry milk. The method involves dissolving a sample in water, adding a phenolphthalein indicator, and titrating the solution with a standardized 0.1N sodium hydroxide solution until a faint pink color persists. The ml of sodium hydroxide required is then used to calculate the percentage of acid as lactic acid. The method uses common laboratory equipment and reagents.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    186 views1 page

    Aacc 02-31

    Uploaded by

    Елена Старинова
    This document describes a method for measuring the titratable acidity or acid content of nonfat dry milk. The method involves dissolving a sample in water, adding a phenolphthalein indicator, and titrating the solution with a standardized 0.1N sodium hydroxide solution until a faint pink color persists. The ml of sodium hydroxide required is then used to calculate the percentage of acid as lactic acid. The method uses common laboratory equipment and reagents.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Acidity AACC Method 02-31

    Page 1 of 1

    Titratable Acidity
    Final approval April 13, 1961; Reapproval November 3, 1999

    Objective
    Titratable acidity or acid content (ml) is measured by the amount of 0.1N
    NaOH (ml) required to neutralize an acid solution. The method is applicable to
    nonfat dry milk.

    Apparatus
    1. Buret graduated in 0.1-ml divisions.
    2. Casserole, white glazed porcelain, 50-ml capacity.
    3. Pipet, volumetric, 17.6 ml.

    Reagents
    1. Phenolphthalein indicator, 1%. Prepare by dissolving 1 g phenolphthalein in
    50 ml 95% ethyl alcohol and diluting to 100 ml with water.
    2. NaOH, 0.1N standardized solution (Method 70-70).

    Procedure
    1. Dissolve and disperse 10 g of sample in 100 ml water and mix thoroughly.
    Let stand approximately 1 hr. Stir gently, and then pipet 17.6 ml into porcelain
    casserole. Rinse out same pipet with 17.6 ml water and add this to sample in cas-
    serole.
    2. Add 0.5 ml phenolphthalein indicator and titrate with standardized 0.1N
    NaOH until faint pink color persists for 30 sec. Caution: NaOH is extremely
    caustic. Protect skin and eyes. Add pellets to water and not water to pellets.

    Calculation
    ml 0.1N NaOH required
    Percent acid as lactic acid =
    20

    References
    AOAC International. 1995. Official Methods of Analysis of AOAC International, 16th ed. Method
    947.05. The Association, Arlington, VA.

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