Bio-Rad Laboratories IMMUNOHEMATOLOGY

DiaCent-12
Service Manual
V5.0-10/2010

The Complete Solution for Safe Transfusion

Identification

Document
DiaCent-12 Service Manual

Version : 5.0 Version Date Comment

: H005241 1.0 First edition

2.0 Second edition

3.0 April 04 Third edition

4.0 25.02.05 Fourth edition,

adaptation of the new
layout
Manufacturer
DiaMed GmbH 4.1 04.05.05 Changes in the spare
parts
1785 Cressier FR
5.0 25.10.10 Brand Change
Switzerland

Tel. +41 (0)26 67 45 111

Fax +41 (0)26 67 45 145

Copyright ©

The reproduction, even partially, of this document is forbidden. No part may be copied in any form, and it
may not be used, edited nor transmitted by any electronic means (photocopy, photography, magnetic
supports or other recording processes), without the written authorization of Bio-Rad. All rights and
particularly reproduction, translation, edition, distribution and also industrial property and recording are
reserved.

Printed in Switzerland

DiaCent-12 Service Manual • © Bio-Rad

 Table of Contents

1 Generalities .........................................................................................................................7
1.1 Appropriate use ...........................................................................................................7
1.2 Glossary .......................................................................................................................8
1.2.1 Persons............................................................................................................8
1.2.2 Product ............................................................................................................9
1.3 Typographical conventions .........................................................................................9
1.3.1 Description ......................................................................................................9
1.3.2 Command ........................................................................................................9
1.3.3 Procedure ........................................................................................................9
1.3.4 Cross reference ............................................................................................10
1.3.5 List of items..................................................................................................10
1.3.6 Troubleshooting.............................................................................................10
1.3.7 Warnings........................................................................................................11
1.3.8 Recommendation and Note.........................................................................12

2 Safety .................................................................................................................................13
2.1 Introduction ................................................................................................................13
2.1.1 Principle.........................................................................................................13
2.1.2 Importance of the safety instructions.........................................................13
2.1.3 Disregarding the safety rules ......................................................................13
2.2 Environmental conditions ..........................................................................................14
2.3 General safety instructions .......................................................................................14
2.3.1 Observations and informations....................................................................15
2.4 Special safety instructions........................................................................................16
2.4.1 Safety labels .................................................................................................16

3 Description ........................................................................................................................17
3.1 General description ...................................................................................................17
3.1.1 Main description ...........................................................................................17
3.1.2 Upper and lower parts of the housing ......................................................18
3.2 Technical data ...........................................................................................................19
3.2.1 Performance data .........................................................................................19
3.2.2 Physical data ................................................................................................19
3.2.3 Suitable sample tubes .................................................................................20
3.2.4 Dimensions and weights..............................................................................20
3.2.5 Environmental conditions .............................................................................20
3.2.6 Emissions ......................................................................................................20

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Table of Contents

4 Commissioning................................................................................................................. 21
4.1 Checking the shipping documents.......................................................................... 21

5 Checks and adjustments ................................................................................................. 23

5.1 Equipment required .................................................................................................. 24
5.1.1 Technical requirements ................................................................................ 24
5.1.2 Cleaning agent ............................................................................................. 24
5.1.3 Protective equipment ................................................................................... 24
5.1.4 Measuring instruments................................................................................. 24
5.2 Checking the power supply..................................................................................... 25
5.2.1 Measuring the primary voltage on the power supply board................... 26
5.2.2 Measuring the secondary voltage on the power supply board .............. 26
5.2.3 Measuring the input voltage on the controller board .............................. 26
5.3 Functional check/Calibration .................................................................................... 27
5.3.1 Checking keyboard and display ................................................................. 27
5.3.2 Checking the lock........................................................................................ 29
5.3.3 Adjusting the vibration detection for centrifuges with SN from 892001001
30
5.3.4 Adjusting the vibration detector for centrifuges with SN up to 892001001
33
5.3.5 Checking nominal speed and centrifugation time .................................... 35
5.3.6 Checking mobility/position of the tube holders ........................................ 39
5.3.7 Checking the motor running time .............................................................. 40

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 Table of Contents

6 Maintenance......................................................................................................................43
6.1 Maintenance plan ......................................................................................................43
6.2 Cleaning and decontamination.................................................................................44
6.3 Disassembly ...............................................................................................................45
6.3.1 Opening the lid and removing the centrifuge head..................................45
6.3.2 Disassembling the centrifuge housing ........................................................46
6.3.3 Separating the upper part from the lower part of the housing ..............47
6.4 Assembly....................................................................................................................48
6.5 Mechanical parts replacement .................................................................................48
6.5.1 Lid hinge .......................................................................................................48
6.5.2 Right hinge....................................................................................................49
6.5.3 Left hinge ......................................................................................................50
6.5.4 Lid..................................................................................................................51
6.5.5 Locking pin ...................................................................................................52
6.5.6 O-ring.............................................................................................................53
6.5.7 Silicone buffer ...............................................................................................53
6.5.8 Shaft adapter ................................................................................................54
6.5.9 V-ring .............................................................................................................55
6.5.10 Rubber feet ...................................................................................................56
6.5.11 Replacing the magnet in the centrifuge head...........................................57
6.6 Electronical and electrical parts replacement .........................................................58
6.6.1 Replacing the IES switch assembly ...........................................................58
6.6.2 Replacing the mains fuses ..........................................................................59
6.6.3 Power supply board.....................................................................................60
6.6.4 Replacing the controller board....................................................................61
6.6.5 Replacing the membrane keyboard ............................................................62
6.6.6 Replacing the LCD board............................................................................63
6.6.7 Replacing the lock .......................................................................................64
6.6.8 24V motor/vibration sensor..........................................................................66

7 Spare parts ........................................................................................................................69

7.1 Spare parts ................................................................................................................69
7.1.1 Use and application .....................................................................................69
7.1.2 Ordering spare parts ....................................................................................69
7.2 Lists of spare parts ..................................................................................................70
7.2.1 Lower part of housing .................................................................................70
7.2.2 Upper section ...............................................................................................72

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Table of Contents

8 Decommissioning............................................................................................................. 77
8.1 Disposal of waste equipment .................................................................................. 77

9 Appendix ........................................................................................................................... 79
9.1 Equipment.................................................................................................................. 79
9.1.1 Standard accessories................................................................................... 79
9.1.2 Special accessories ..................................................................................... 79
9.2 Technical Documentation ......................................................................................... 79
9.2.1 User Manual ................................................................................................. 79
9.2.2 Service Manual (english only) ..................................................................... 79
9.3  Conformity.......................................................................................................... 80
9.4 Documents / Forms.................................................................................................. 80
9.4.1 Reception form............................................................................................. 81
9.4.2 Installation protocol...................................................................................... 82
9.4.3 Decontamination certificate ......................................................................... 83
9.5 Maintenance record .................................................................................................. 85
9.6 Identification plate..................................................................................................... 87
9.7 Block diagram ........................................................................................................... 88

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 Generalities 1

Chapter contents

This chapter contains basic information on the structure of the document, its specifications and on
the documentation.

Preface

This manual is intended for authorized and qualified persons who carry out servicing activities on
the DiaCent-12.

It contains all the information necessary for servicing the following centrifuge.

Type Part Number

DiaCent-12 840000

The DiaCent-12 is normally supplied with a User Manual in the operator language.

The Service Manual is available in English but only to authorized technical staff.

In case of inquiries with the manufacturer or your Bio-Rad representative, always specify the full
type and serial number of the DiaCent-12 to be repaired. You will find this information on the
identification plate on the back panel of the DiaCent-12.

1.1 Appropriate use

The DiaCent-12 may be used only for the centrifugation of blood and blood components contained
in tubes.

It may be used only by authorized personnel in medical laboratories and not in the immediate
vincinity of patients. Use at more than 2000 m above sea level is prohibited.

The centrifugation of materials other than those described in the User Manual and in the Service
Manual is forbidden (e.g. non-approved hazardous substances). Any other application is considered
as improper use of the centrifuge.

For this reason, the information in this Service Manual, in particular the safety instructions, must be
strictly adhered to.

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1 Generalities

1.2 Glossary
The following terms, among others, are used in the present Service Manual.

1.2.1 Persons

Manufacturer

The manufacturer of the DiaCent-12 is :

DiaMed GmbH, CH-1785 Cressier sur Morat, Switzerland.

Operator

The operator is the owner of a centrifuge, both when using it as its owner and when transferring
it to a third-party.

Personnel

The personnel gathers persons who have any kind of activity with the DiaCent-12 and who are
qualified in accordance with the manufacturer’s requirements and who are consequently authorised.

Technical personnel

The term «technical personnel» designates the duly trained persons, who are allowed to perform
specific tasks on the DiaCent-12.

For instance, an electrician is designated as technical personnel for the activities linked to wiring
the DiaCent-12 to the electrical network.

Personnel qualifications

Several kinds of personnel qualifications are required in order to perform the activites related to the
DiaCent-12. These qualifications are described in the corresponding sections of the present Service
Manual.

The personnel qualifications define the minimal requirements which must be met by the authorised
personnel.

Serious injury

A serious injury is an injury (ICAO definition) which is sustained by a person in an accident and
which :

• requires hospitalization for more than 48 hours, commencing within seven days from the date
the injury was received;
• results in a fracture of any bone (except simple fractures of fingers, toes, or nose);
• involves lacerations which cause severe hemorrhage, nerve, muscle or tendon damage;
• involves injury to any internal organ;
• involves second or third degree burns, or any burns affecting more than 5 per cent of the body
surface;
• involves verified exposure to infectious substances or injurious radiation.
Light injury

Any injury not corresponding to the definition of a serious injury is considered as a light injury.

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 Generalities 1

1.2.2 Product

This is the DiaCent-12 distributed by the manufacturer.

Centrifuge

The term “centrifuge” is used for the DiaCent-12 for 100–240 VAC (Part no. 840000) made by the
manufacturer.

1.3 Typographical conventions

The following styles are used in this manual.

1.3.1 Description

This style, use in conjunction with illustration numbers, is preceded with the corresponding
numbers:

Example:

(1) First element

(2) Second element
(3) Etc...

1.3.2 Command

Any software command, button, function key, window, icon, option, tab, checkbox, selection box,
article, menu, tool bar, field and section used in this document is represented by a bold italic font.

Example:

The Exit command allows to quit the software.

1.3.3 Procedure

Each procedure step to be carried out step-by-step by the user is preceded by a letter.

Example:

A. Open the drawer.

B. Put the microplate into position as shown.
C. Close the drawer.
1.3.3.1 Procedure result
A procedure result is shown by the following symbol .
Example:

A. Click on the Parameters button.

The parameter window is displayed.

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1 Generalities

1.3.4 Cross reference

This style is used to help the user find complementary information linked to the current subject.

Example:

 See section “4.2.4 Sensor Board Positioning” on page 4-7.

1.3.5 List of items

This style is used in order to display a list of elements.

Example:

• item 1;
• item 2;
• item 3.

1.3.6 Troubleshooting

The complete description along with the error message, the explanation and the remedy appears
as follows:

Problem
- Explanation, possible cause

 Remedy

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 Generalities 1

1.3.7 Warnings

In relation to the importance of the warning and its related risks, three warning styles are defined.

The safety aspects are used in accordance with the requirements contained in the following norms:

• ANSI Z535.4;
• ISO 3864 and ISO 3864-1:2002.

1.3.7.1 Danger
Used to designate an imminent and dangerous situation which, if not avoided, may lead to death
or serious injury.

The primary risk is given in capital letters below the DANGER symbol. However, mentioning a
specific risk does not exclude the presence of subsidiary risks.

Example:

ELECTROCUTION
Never touch an exposed electrical wire. A contact with an electrical wire may cause an
electrocution.

1.3.7.2 Warning
Used to designate a potentially dangerous situation which, if not avoided, may lead to death or
serious injury.

Example:

Always disconnect the electrical cord before opening the instrument. A contact with an
exposed electrical wire may cause an electrocution.

1.3.7.3 Caution
Used to designate a potentially dangerous situation which, if not avoided, may lead to light injury
or cause equipment damage.

Example:

Do not manipulate a broken mirror with bare hands as this operation may result in
cuts.

The use of the CAUTION sign without the warning triangle means that the only risk consists in
equipment damage.

Example:

The use of other cleaning agents or hard objects may damage the instrument. Do not use other
cleaning agents or products without obtaining first the authorisation of the manufacturer.

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1 Generalities

1.3.8 Recommendation and Note

When complementary information is required and if their non-compliance only leads to minor
inconveniences, recommendations and notes are given.

1.3.8.1 Recommendation
Used to designate a prefered procedure or a recommended practice. Bio-Rad denies any
responsibility in case of non-compliance with the recommendations.

Example:

Check that the instrument is closed before switching it on.

1.3.8.2 Note
Used to accompany a general remark or a purely informative comment.

Example:

The reassembly of the instrument is performed in the opposite order of its disassembling.

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 Safety 2

Chapter contents

This chapter summarizes the general safety instructions necessary for the proper functionning of
the DiaCent-12. They must be observed by personnel operating the centrifuges.

Before any intervention on the DiaCent-12, make sure you have read and understood this
chapter.

2.1 Introduction
2.1.1 Principle

In case of unclear information, please contact the manufacturer or your Bio-Rad dealer.

2.1.2 Importance of the safety instructions

Every safety and protection instruction which can be found in this manual must be adhered to in
order to avoid personnel injuries, property damages or environmental pollution.

In a similar manner, the legal bylaws, the measures in prevention of accidents and for the
protection of the environment, as well as the recognised technical rules aiming at appropriate and
safe working conditions which are applied in the country and at the place of use of the DiaCent-
12 instrument must be adhered to.

2.1.3 Disregarding the safety rules

Disregarding the safety rules, as well as existing legal and technical regulations, may lead to
accidents, property damages or to environmental pollution.

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2 Safety

2.2 Environmental conditions

The DiaCent-12 must not be located near a water tap or any other source of water.

This system may only be used in closed rooms and never in the immediate environment of
patients.

The electrical safety of the DiaCent-12 instrument is only guaranteed if the electrical installation
is conform to the reglementation related to medical-use buildings and laboratories and if this
installation works properly.
The DiaCent-12 may not be used in buildings prone to explosion hazards.

The DiaCent-12 must be kept away from any interference source.

The DiaCent-12 may not be stored under exposure to the direct sunlight, to heat, to dust or to
an excessive humidity (only use the instrument in a clean laboratory environment).

2.3 General safety instructions

ELECTROCUTION
During maintenance operations, when the DiaCent-12 is powered and its cover is removed, the
instrument may not be left without watch.

Maintenance and repairs may only be performed in conformance with the instructions and by
the technical personnel authorised by the manufacturer.

The sole possession of the Service Manual does not allow the personnel to perform any kind of
repair on the DiaCent-12.

The manufacturer offers special training courses for repairing the DiaCent-12. You can obtain
information from your Bio-Rad representative or the manufacturer.

Take into account all the warnings and follow all the instructions displayed on the DiaCent-12
and which are printed in the documentation.

It is mandatory to use the products specified in the present Service Manual to clean the
DiaCent-12. If you plan to use another product, only do so after having obtained the
authorisation of the manufacturer.

The DiaCent-12 may only be connected to appropriate electrical sources given in section “3.2.2
Physical data” on page 3-19.

Using materials other than those defined in the User Manual (unauthorised dangerous goods
for instance) is forbidden. Breaking this rule will be considered by the manufacturer as guilty
negligence.

Never try to use spare parts other than those authorised by the manufacturer of the DiaCent-12.

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 Safety 2

The DiaCent-12 must be used on an appropriate table.

Ensure that the ventilation around the DiaCent-12 is sufficient to avoid any excessive heating. A
space of 300 mm all around the DiaCent-12 must be left clear of any obstacle.

See User Manual, chapter Commissioning for more information about the installation site.

The DiaCent-12 must only be used with accessories approved by the manufacturer (example:
centrifuge head).

Do not operate the centrifuges in the case of damage or switch off immediately. Switch off the
power switch and remove the power plug from the mains! Mark the centrifuge as defective to
prevent further use.

The DiaCent-12 may not be used in combination with other machines. The manufacturer’s
agreement must be obtained before using the DiaCent-12 in conjunction with other equipment.

The centrifuges must not be operated with an open protective lid, as otherwise fluctuations in
the temperature may occur which can affect test results.

Switch off the power switch and remove the power cord from the mains when cleaning the
centrifuge or when replacing the fuse.

Never let any liquid enter the system. In case of liquid spill inside the instrument, act
immediately as follows:

A. Switch off the instrument using the main switch.

B. Unplug the power cord.
C. Dry up the instrument.
D. Clean and decontaminate the instrument.
E. Check all its electrical functions.

For further information, please contact your Bio-Rad dealer or the manufacturer.

2.3.1 Observations and informations

In case of defective operation or any other technical incident for which no remedy is described in
this manual, please contact immediately the manufacturer or your dealer.

Safety measures ordered by the manufacturer are mandatory.

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2 Safety

2.4 Special safety instructions

Safety instructions are inserted at certain points in this manual to indicate special hazards which
occur only in certain situations.

They should be observed in the same way as the general safety instructions contained in this
chapter.

2.4.1 Safety labels

Sign Description

Biohazard

The sticker on top of the housing has the following meaning:

Equipment can be potentially infectious due to the samples or reagents used.

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 Description 3

3.1 General description

3.1.1 Main description

Locking pin

Lid

Centrifuge head

O-ring

Hinge

Shaft adapter
Tub

Membrane
keyboard
V-ring

Housing

Fig. 3-1: Overall view

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3 Description

3.1.2 Upper and lower parts of the housing

Motor

Ground connection

Vibration sensor
(below motor)

Lock assembly

LCD board

Controller
board

Ground connection

Noise suppressor

IES switch
Power supply assembly
board

Fig. 3-2: Upper and lower parts of the housing

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 Description 3

3.2 Technical data

3.2.1 Performance data

Maximum speed ................................................3000 rpm

Speed tolerance.................................................± 1 %
Acceleration........................................................10006 g
Acceleration tolerance .......................................± 1 %
Minimum centrifugation time ............................1 s
Maximum centrifugation time ...........................60 min
Centrifugation time tolerance............................± 1 %

With centrifuge head 12

Number of tubes ...............................................12

Diameter of tubes..............................................10...12 mm
Length of tubes .................................................65...83 mm

With centrifuge head 6

Number of tubes ...............................................6
Diameter of tubes..............................................10...16.5 mm
Length of tubes .................................................80...107.5 mm

3.2.2 Physical data

Mains voltage.....................................................100...240 VAC

Mains frequency ................................................50...60 Hz
Max. power consumption .................................70 VA
Mains fuse for 100 - 240 VAC........................2 A slow blow

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3 Description

3.2.3 Suitable sample tubes

L
3.2.3.1 With centrifuge head 12
Glass or plastic tubes
D
L = ............................................ 65...83 mm
D =............................................ 10...12 mm
3.2.3.2 With centrifuge head 6 D
Glass or plastic tubes

L = ............................................ 80...107.5 mm Fig. 3-3: Suitable sample tubes

D =............................................ 10...16.5 mm

3.2.4 Dimensions and weights

A = width ............................... 26 cm
B = depth ............................... 32 cm
C = height .............................. 18 cm D

D = height with lid open ........ 41 cm

Weight of centrifuge .............. 4.6 kg C
Weight of centr. head 12 ....... 0.2 kg
Weight of centr. head 6 ......... 0.6 kg
B A

Fig. 3-4: Dimensions and weights

3.2.5 Environmental conditions

Ambient temperature Operation .......................15 to +40 °C

Storage ...............................................................-10 to +65 °C

Ambient air humidity

Non-condensing .................................................max. 85 % rh

Altitude above sea level....................................not above 2000 m

3.2.6 Emissions

Noise .................................................................< 70 dB(A)

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 Commissioning 4

4.1 Checking the shipping documents

The following shipping documents should be included with the centrifuge :

• Decontamination certificate
If a decontamination certificate is not included with the centrifuge or if it is suspected that a
proper decontamination has not been made, perform a decontamination.
A reproduction master of this form is contained in the section “9.4 Documents / Forms” on
page 80.

If the centrifuge has been contaminated with samples or reagents, there is a risk of infection.
Observe all applicable safety regulations, wear protective gloves, and carry out a
decontamination as described in section “6.2 Cleaning and decontamination” on page 44.

• Bill of delivery
List of supplied parts
• Problem description
• Maintenance report
The maintenance report contains all performed maintenance procedures, inspections, and
replacements. If a maintenance report is not included with the shipment, you can find a
reproduction master of this form in the section “9.5 Maintenance record” on page 85.

Restore the lab settings before returning the centrifuge.

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4 Commissioning

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 Checks and adjustments 5

Chapter contents

This chapter describes the checks to be performed by authorized qualified personnel for
troubleshooting.

- Observe chapter “2 Safety” as well as all safety instructions contained in this chapter, section
“7 Spare parts” on page 7-45.
- The part numbers for ordering spare parts are contained in the spare parts catalogue.

ELECTRIC SHOCK
Do not touch any metal part if the unit is switched on and the housing is open.
Do not leave units unattended with the power switched on and the housing open.

Use only original spare parts.

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5 Checks and adjustments

5.1 Equipment required

5.1.1 Technical requirements

An electro-technical workshop with all the appropriate technical equipment is a requirement for the
orderly execution of maintenance work.

5.1.2 Cleaning agent

The best liquid cleaning agent is a 70% ethyl alcohol solution.

Other cleaning aids are soft, lint-free cloths, cotton buds or swabs.

Hard objects are not suitable for cleaning purposes.

5.1.3 Protective equipment

Protective gloves suitable for laboratory use.

5.1.4 Measuring instruments

To carry out various checking and adjusting operations you will need an optical tachometer
(stroboscope). The manufacturer has the appropriate equipment in stock. You will also need a
commercial stopwatch.
B
RO
-ST
BO
UR
TO

Fig. 5-1: Stroboscope (left) and stopwatch (right)

For the electrical measurements you will need a multimeter with at least the following measurement
ranges :

• 230/110 VAC;
• 24 VDC;
• 2 A.

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 Checks and adjustments 5

5.2 Checking the power supply

Material required

• multimeter.

ELECTRICAL SHOCK
With the housing opened and the unit switched on, the metal parts carry the mains voltage.
Do not touch any metal parts.

Procedure

A. Open the centrifuge housing as described in section “6.3.2 Disassembling the centrifuge housing”
on page 6-46.

Pin 1

Pin 4

L
(1)
N

(2)

(4)
Pin 3
Pin 1

(3)

Fig. 5-2: Checking the power supply

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5 Checks and adjustments

5.2.1 Measuring the primary voltage on the power supply board

A. Disconnect the connector (1).

B. Switch on the centrifuge.
C. Measure the primary voltage (input) at connector (4) between Pin N and Pin L.

It should be 100 VAC to 240 VAC, depending on the country of use.

D. Switch off the centrifuge.

E. Reconnect the connector.

5.2.2 Measuring the secondary voltage on the power supply board

A. Switch on the centrifuge.

B. Measure the secondary voltage (output) at connector (1) between Pin 1 and Pin 4.

It should be 24 VDC.

C. Switch off the centrifuge.

5.2.3 Measuring the input voltage on the controller board

A. Switch on the centrifuge.

B. Measure the input voltage at connector (3) between Pin 1 and Pin 3.
C. If the input voltage is lower than 24 VDC, adjust the voltage to 24 VDC using potentiometer (2).
D. Switch off the centrifuge.

E. Assemble the centrifuge as described in section “6.4 Assembly” on page 6-48.

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 Checks and adjustments 5

5.3 Functional check/Calibration

The display examples in this chapter are not necessarily identical to the actually displayed
texts.

5.3.1 Checking keyboard and display

DiaCent 12 V5.2b
Press the individual keys one after the other and
check the corresponding display according to
the list below:
P0 2000 rpm 0:15

A. Switch on the centrifuge.

Fig. 5-3: Display

B. Close the lid and press the START/STOP key

(centrifugation will be started in program P0).
** START **

While
Fig. 5-4: Display
the centrifuge is accelerating, the
display continuously shows the speed.
1900 rpm 0:15

During
Fig. 5-5: Display
centrifugation, the nominal time is
counted down in the display.
2000 rpm 0:03

Fig. 5-6: Display

327 rpm STOP

The centrifuge is now coasting to a stop. The
display reads:

327 rpm STOP

Fig. 5-7: Display

P0 2000 rpm 0:15

After 3 to 4 seconds, the lid will open and the
display will show the selected program again.
Fig. 5-8: Display

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5 Checks and adjustments

C. With the lid open, press the START/STOP key

(requires that program P0 is selected).
Display in g

P0 447 g 0:15

or

Display in rpm

P0 2000 rpm 0:15

Fig. 5-9: Display

D. With the lid open, press the SPEED/TIME key

(requires that program P0 is selected)
P0 2000 rpm 0:15

P1 2000 rpm 0:20

P2 2000 rpm 0:30

.
.
.

P9 3000 rpm 0:50

Fig. 5-10: Display

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 Checks and adjustments 5

5.3.2 Checking the lock

A. Close the lid.

B. Switch on the centrifuge.

The lid of the centrifuge will open automatically after about 4 seconds.
C. If packing material is still present in the tub, remove it now.
D. Close the lid again.

It must not be possible to lift the lid.

E. Press the START/STOP key to start the centrifugation.

F. Press the START/STOP key when the program is running.

It should only be possible to open the lid by pressing the START/STOP key after the motor has
come to a standstill.

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5 Checks and adjustments

5.3.3 Adjusting the vibration detection for centrifuges

with SN from 892001001

Corresponds to EPROM version > 5.3

Material required

• 12 water-filled sample tubes, filling level:

about 3/4;
• screwdriver size 0.

5.3.3.1 Checking the tilt sensor

A. Switch on the centrifuge.

The lid of the centrifuge will open

automatically after about 4 seconds.

B. Load the centrifuge head with 4 water-filled

tubes on adjacent positions (centrifuge head 12).
C. Close the lid.
(1)
D. Carefully move the centrifuge over the edge of
the table until you are able to adjust the vibration Fig. 5-11: Checking the tilt sensor
detector with the screwdriver.
E. Turn the potentiometer fully clockwise (seen from
above) (1), in order to adjust the sensitivity of the
vibration detector to a minimum.
F. Press the START/STOP key.

The centrifuge should stop and the following

text will be displayed:

The lid opens and an acoustic signal appears.

Tilt Vibrations !

G. Continue with the adjustment of the vibration

detector as described in section “5.3.3.2 Check the head
Adjusting the vibration detector” on page 5-31.

Fig. 5-12: Display

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 Checks and adjustments 5

5.3.3.2 Adjusting the vibration detector

A. Load the centrifuge head with 2 water-filled
tubes on positions 1 and 6.
B. Switch off the centrifuge.
C. Close the lid.
D. Press both keys simultaneously while switching
on the centrifuge again.

When gently tapping against the centrifuge you

should hear an acoustic signal.

E. Press the START/STOP key.

F. Confirm the setting of “Vib.y-Offset: 0” with the
START/STOP key, too.

The following text will be displayed:

G. Press the START/STOP key. Vibr. sensor adj.

create min Vibr.

pl. press START

Fig. 5-13: Display

The following text will be displayed:

H. While centrifuging, hold the centrifuge with one 3000 rpm 0
hand and adjust the vibration detector to a value
of about 35–40 (the smaller the value the less
sensitive). Check the value when the centrifuge is
Fig. 5-14: Display
positioned completely on the table again.
I. Press the START/STOP key.
J. Switch off the centrifuge and switch it on again.
K. Load the centrifuge head with 2 water-filled
sample tubes on adjacent positions.
L. Select the program P1 (= 2000 rpm) with the
SPEED/TIME key.
M. Close the lid and press the START/STOP key.

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5 Checks and adjustments

3000 rpm 0
The centrifuge should stop at a speed between
1600 rpm and 1900 rpm.
Fig. 5-15: Display

The following text will be displayed:

N. Select the program P5 (= 3000 rpm) with the
SPEED/TIME key.

O. Close the lid and press the START/STOP key.

! Vibrations !
The centrifuge should stop at a speed between
1900 rpm and 2300 rpm.
Fig. 5-16: Display

The following text will be displayed:

P. Repeat the adjustment of the vibration detector
until the correct setting is obtained.
Q. Load the centrifuge head with water-filled sample
tubes on all 12 positions.
R. Check the correct speed using P1 and P5 by
means of a stroboscope.

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 Checks and adjustments 5

5.3.4 Adjusting the vibration detector for centrifuges

with SN up to 892001001

Corresponds to EPROM version < 5.3

Material required

• 12 water-filled sample tube, filling level: to 1

cm below the upper rim;
• screwdriver size 0.

Procedure

A. Switch on the centrifuge.

The lid of the centrifuge will open

automatically after about 4 seconds.

B. Fill the centrifuge head 12 with 11 water-filled

tubes.
C. Close the lid.
D. Carefully move the centrifuge over the edge of
the table until you are able to adjust the vibration
detector with the screwdriver.
E. Select the program “P0 2000 rpm 5:00”.

Programming is described in the User Manual,

section “Setting / changing the pre-selection
programs”.
(1)

F. Turn the potentiometer fully clockwise (seen from Fig. 5-17: Adjusting the vibration detector
above).
G. Press the START/STOP key.

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5 Checks and adjustments

H. Wait until the centrifuge has reached its nominal

speed of 2000 rpm.
I. Turn the potentiometer counterclockwise until the
vibration detector is activated and the centrifuge
starts decelerating.

The following text will be displayed: ! Vibrations !

J. Turn the potentiometer slightly more

counterclockwise. Fig. 5-18: Display
K. After the centrifuge has come to a stop, restart
the centrifugation by pressing the START/STOP
key.
L. Repeat this procedure until the alarm is activated
between 1600 rpm and 2000 rpm.
M. Insert 12 water-filled tubes.
N. Press the START/STOP key.

The centrifuge should reach its nominal speed of 2000 rpm.

If this is not the case, readjust the vibration detector.

O. After adjusting the vibration detector at 2000 rpm, check this setting at 3000 rpm.

The vibration detector should again trip between 1600 rpm and 2000 rpm when using only 11
water-filled tubes.

If this is not the case, readjust the vibration detector.

34/90 DiaCent-12 Service Manual • © Bio-Rad

 Checks and adjustments 5

5.3.5 Checking nominal speed and centrifugation time

Material required

• 12 water-filled sample tube, filling level: to 1

cm below the upper rim;
• optical speed measuring instrument
(stroboscope) (Part No. 0471003);
• stop watch.

5.3.5.1 Checking nominal speed

A. Switch on the centrifuge with the power switch
on the rear panel.

The lid will open.

B. Fill the centrifuge head with the 12 water-filled
tubes.
C. Close the lid.

It should audibly click shut.

D. Place the activated stroboscope on the lid
exactly above the tube holder. Set a speed of
2000 rpm on the stroboscope.

Observe the operating instructions of the

stroboscope. Fig. 5-19: Checking nominal speed

E. Create a program for a speed of 2000 rpm and

a centrifugation time of 5:00 minutes.
Programming is described in the User Manual, P0 2000 rpm 5:00
section “Setting / changing the pre-selection
programs”.
F. Press the START/STOP key.

The following text will be displayed: ** START **

While the centrifuge is accelerating, the display 567 rpm START

continuously shows the speed.

2000 rpm 4:59

Fig. 5-20: Display

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5 Checks and adjustments

G. As soon as the centrifuge has stabilised at

nominal speed, adjust the stroboscope until the
centrifuge head is optically stationary.

The nominal speed of 2000 rpm has been

reached when the timer begins counting down.

H. Compare the speed shown on the stroboscope

with the nominal speed. 45˚

The maximum admissible tolerance is ± 1 % rpm.

I. Note the speed obtained.

J. Make a visual check through the transparent lid
to make sure that all tube holders are fully
swung out to 45 degrees. Fig. 5-21: Tube holders at 45 degrees

K. Enter the test result into the maintenance report.

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 Checks and adjustments 5

5.3.5.2 Checking centrifugation time

A. Switch on the centrifuge with the power switch
on the rear panel.

The lid will open.

B. Fill the centrifuge head with the 12 water-filled
tubes.
C. Close the lid.

It should audibly click shut.

D. Create a program for a speed of 2000 rpm and
a centrifugation time of 5:00 minutes.
Programming is described in the User Manual, P0 2000 rpm 5:00
section “Setting / changing the pre-selection
programs”.
E. Press the START/STOP key.

The following text will be displayed:

** START **

567 rpm START

While the centrifuge is accelerating, the display
continuously shows the speed.

2000 rpm 4:59

Start of centrifugation time

The nominal speed has stabilised as soon as the

display shows the centrifugation time instead of
“START”. 2000 rpm 1:23

F. As soon as the nominal speed of the centrifuge

has stabilised, start the stop watch.
Fig. 5-22: Display

During centrifugation, the display continuously

counts down the remaining centrifugation time.

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5 Checks and adjustments

End of centrifugation time

The nominal time has elapsed as soon as the 1798 rpm STOP
display shows “STOP” instead of the time.

G. As soon as the nominal time has elapsed, stop

the stop watch. Compare the time shown on the
stop watch with the nominal time.

The maximum admissible tolerance is ± 1 %.

Wait please

H. Note the nominal time obtained.

P0 2000 rpm 5:00

Meanwhile the centrifuge will coast to a stop.
The dual-tone alarm will sound, and the
following text will be displayed:
Fig. 5-23: Display

After 3 to 4 seconds, the lid will open and the

display will show the selected program again.

I. Enter the test result into the maintenance report.

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 Checks and adjustments 5

5.3.6 Checking mobility/position of the tube holders

The tube holders are subjected to high forces

during operation. For this reason, they need to
be checked for easy movement and full 45
degree swing angle.

A. Place the centrifuge head into the centrifuge.

B. Manually swing each tube holder through 45°.

Release the tube holder.

The spring action should immediately return it

to its neutral position.
45˚

If it is not possible to move one or several

holder(s) through its/their full 45° swing angle or
if the movement appears sluggish, discontinue
the use of this centrifuge head for centrifugation.

7
8 6
9

To make sure that each tube holder is tested,

10

they are numbered on the centrifuge head.

11

12
2
1

1
C. Enter the test result into the maintenance report.

Fig. 5-24: Checking mobility/position of the tube

holders

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5 Checks and adjustments

5.3.7 Checking the motor running time

A. Switch on the centrifuge.

B. Insert an empty centrifuge head.
C. Close the lid.
D. Switch off the centrifuge.
E. Press the keys START/STOP and SPEED/
TIME at the same time while switching on the
centrifuge again. DiaCent 12

The display shows the following texts one

after the other:
Test program

Tilt Sensor Test

Shake care Cent.

F. Release the keys START/STOP and SPEED/
TIME without shaking the centrifuge.
G. Press the START/STOP key.

The display shows the following texts one

after the other:
Vib. y-Offset 0

Vib. sensor Adj.

Create min. vibr.

pl. press START

Fig. 5-25: Display

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 Checks and adjustments 5

H. Press the START/STOP key twice.

The display shows the following texts one

Motor Cont. Test
after the other:

pl. press START.

I. Press the START/STOP key.

The centrifuge will accelerate to 3000 rpm and

decelerate again after about 4 seconds. The
display shows the following texts one after the Wait please
other:

364 rpm

2948 rpm 0:02

3000 rpm 0:01

3000 rpm 0:00

568 rpm STOP

Wait please

Fig. 5-26: Display

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5 Checks and adjustments

The acceleration and deceleration processes are alternated.

J. Switch off the centrifuge after 2-3 cycles.

42/90 DiaCent-12 Service Manual • © Bio-Rad

 Maintenance 6

Chapter contents

This chapter describes the maintenance procedures to be performed by authorized qualified

personnel for troubleshooting.

- Observe chapter “2 Safety” as well as all safety instructions contained in this chapter.
- The part numbers for ordering spare parts are contained in the spare parts catalogue, section
“7 Spare parts” on page 69.

ELECTRIC SHOCK
Do not touch any metal part if the unit is switched on and the housing is open.
Do not leave units unattended with the power switched on and the housing open.

Use only original spare parts.

6.1 Maintenance plan

The maintenance work described in the maintenance plan below should be carried out by the user
according to the instructions in the maintenance plan and recorded in the maintenance report.

The maintenance steps in the maintenance plan are described in the following chapters. Read
them carefully before starting.

No internal part of the centrifuge may be lubricated nor oiled.

Maintenance plan

Time Activity Carried out by

As required Cleaning Operator

As required Decontamination Operator/
technical support
Weekly Inspecting the seal and lid Operator/
technical support
Weekly Checking the mobility of the sample tube holder Operator/
and springs technical support
Yearly Checking the target speed Technical support
Yearly Checking the centrifugation time Technical support
Yearly Oil the springs of the lid hinges Technical support

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6 Maintenance

6.2 Cleaning and decontamination

If contamination of the centrifuge or centrifuge head occurs, a decontamination procedure must be
performed immediately.

Do not use a cleaning agent which contains chloride or one which crystallises after
drying, for decontamination purposes, as this may damage the centrifuge. If you
intend to use any cleaning agent not listed in the service manual, contact the
manufacturer for approval.
The centrifuge head must not be sterilised.

If the centrifuge has been contaminated with samples or reagents, there is a risk of infection.
Observe all applicable safety regulations and wear protective gloves during the
decontamination.
All cloths, cotton buds, swabs and other material used in the decontamination should be
considered contaminated and thus disposed of.

Material required

• 70% ethanol solution;

• lint-free cloth, cotton bud or swab.

Procedure

A. Remove all components from the tub as described in section “6.3.1 Opening the lid and removing
the centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Disconnect the power plug.
D. Clean the surfaces of the centrifuge head with a lint-free cloth moistened with a 70 % ethanol
solution. The inner surfaces can be cleaned best with cotton buds or swabs moistened with a
70 % ethanol solution.

If the centrifuge head is heavily contaminated (e.g. after breakage of a tube), decontaminate
the centrifuge head by soaking it in a 70 % ethanol solution. Then clean the centrifuge head in
an ultrasonic bath with distilled water if required.

E. Clean the outside of the housing, the tub and the lid (especially the inside face of the lid) with a
lint-free cloth moistened with a 70 % ethanol solution.
F. Allow the centrifuge and centrifuge head sufficient time to dry after decontamination (at least 30
minutes).

Alcohol vapours may accumulate inside the centrifuge if the drying period is too short.
This is a potential fire and explosion hazard
Allow sufficient time for the centrifuge to dry.

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 Maintenance 6

6.3 Disassembly
6.3.1 Opening the lid and removing the centrifuge head

Material required

• hexagon key size 3.

Procedure

A. Switch on the centrifuge.

The lid will open automatically after about 4 seconds.

B. Remove the packing material and the centrifuge head from the tub.

If it is not possible to open the lid in this way, proceed as follows :

A. Switch off the centrifuge

B. Disconnect the power plug. (1)

C. Remove the screw cover (1) over the locking pin.

(2)
D. Loosen and remove the screw (2).
E. Open the lid.
F. Remove the packing material and the centrifuge
head from the tub.
G. Secure the locking pin to the lid with the screw
(2).
H. Reinsert the screw cover (1). Fig. 6-1: Opening the lid

I. After correcting the problem, check the position

of the locking pin and adjust it according to the
instructions in section “6.5.5 Locking pin” on
page 52.

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6 Maintenance

6.3.2 Disassembling the centrifuge housing

Material required

• hexagon key size 5.5.

Open the housing of the centrifuge first. Then separate the upper part from the lower part of
the housing, if required.

6.3.2.1 Opening the centrifuge housing

A. Remove all components from the tub as
described in section “6.3.1 Opening the lid and
removing the centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Disconnect the power plug.
(1)
D. Unscrew and remove the 4 screws (1) in the
bottom of the housing.
E. Lightly lift the upper part of the housing and
place it on the table with the lid pointing
downward. Fig. 6-2: Opening the centrifuge housing

Please observe that the connecting cables are

very short.

Fig. 6-3: Opening the centrifuge housing

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 Maintenance 6

6.3.3 Separating the upper part from the lower part of

the housing (1)

A. Disconnect the ground connector at the motor

and the connectors (1), (2), (3), (4), (5) and (6) on
the controller board.
B. Remove the cable from the cable holder.

(2)
(6)

(5)

(4) (3)

Fig. 6-4: Separating the upper part from the lower

part of the housing

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6 Maintenance

6.4 Assembly

Assembly of the centrifuge is made in the reverse order of disassembly.

Please make sure that all cables and connectors be firmly connected.

Before placing the upper part into the lower part of the housing,

A. Check the routing of the cables (away from the motor, not pinched),
B. Slide the grounding cable below the controller board (do not remove the board) and route the
cable of the vibration detector behind the flat cable.

6.5 Mechanical parts replacement

Spare parts and order numbers are listed in the spare parts catalogue, section “7 Spare parts”
on page 69.

Use original parts only. Otherwise you may cause damage to the centrifuge or put persons at
risk.

6.5.1 Lid hinge

Possible problems

• defective springs;
• defective hinge.
Material required

• hexagon key size 3;

• torx screwdriver T10;
• hinge (Part No. 0241303) with 2 hinge bushings (Part No. 0241310);
• cylindrical pin Inox ø 5 x 60 (Part No. 0241309);
• spring (Part No. 0241314).
Procedure

A. Open the centrifuge housing as described in section “6.3.2 Disassembling the centrifuge housing”
on page 46.
B. Open the centrifuge lid.
C. Remove the 4 screw covers over the hinges.
D. Unscrew and remove the 4 screws and the lid.
E. Place the upper part of the housing on the table with the tub pointing downward.

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 Maintenance 6

6.5.2 Right hinge

F. Unscrew and remove the 4 screws (2) and the 2 mounting plates (1) on the hinge.
G. Pull out the cylindrical pin (3) and remove the hinge with the bearing bushings.

(1)

(2)

(3)

Fig. 6-5: Replacing the lid hinge

H. Insert a new hinge with bearing bushings.

I. Insert the hinge (4) into the upper part of the
housing as shown in the illustration.
J. Secure the hinge with the cylindrical pin, the
mounting plate and the screws (2).

(4)

Fig. 6-6: Replacing the lid hinge

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6 Maintenance

6.5.3 Left hinge

A. Unscrew and remove the 4 screws (2) and the 2 mounting plates (1) on the hinge.
B. Pull out the cylindrical pin (3) and remove the hinge with the bearing bushings and the spring.

(1)

(2)

(3)

Fig. 6-7: Replacing the lid hinge

C. Insert a new spring (4) into the (new) hinge with

bearing bushes (5).
D. Insert the hinge into the upper part of the
housing as shown in the illustration.
(4)
E. Secure the hinge with the cylindrical pin, the
mounting plate and the screws (2). (5)
F. Assemble the centrifuge as described in section
“6.4 Assembly” on page 48.
G. Secure the lid with the screws.
H. Replace the screw covers.
I. Check that the hinges are able to support the
weight of the lid in every position.

Fig. 6-8: Replacing the lid hinge

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 Maintenance 6

6.5.4 Lid

Possible problems

• scratches or cracks in the plexiglas.

Material required

• hexagon key size 3;

• lid (Part No. 0351501).
Procedure

A. Open the centrifuge housing as described in section “6.3.1 Opening the lid and removing the
centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Disconnect the power plug.
D. Remove the 5 screw covers (2) on the outer side of the lid.
E. Unscrew and remove the 5 screws (1) and (3), the locking pin (4) and the lid.

(1)

(2)

(3)
(4)

Fig. 6-9: Replacing the lid

F. Fix the lid with the 4 screws (3).

G. Close the lid.
H. Align the rear edge of the lid with the rear side of the hinge.
I. Secure the lid with the 4 screws (3).
J. Secure the locking pin with the screw (1) and align it as described in section “6.5.5 Locking pin”
on page 52.
K. Replace all screw covers.
L. Check the proper function of the lock as described in section “5.3.2 Checking the lock” on
page 29.

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6 Maintenance

6.5.5 Locking pin

Possible problems

• locking pin bent.

Material required

• hexagon key size 3;

• locking pin (Part No. 0241504).
Procedure

A. Open the centrifuge housing as described in

section “6.3.1 Opening the lid and removing the
centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Remove the screw cover over the locking pin.
D. Unscrew and remove the screw and the locking
pin.
E. Secure a new locking pin with the screw and
align it as shown in the illustration beside.
F. Replace the screw cover.
Fig. 6-10: Replacing the locking pin
G. Check the proper function of the lock as
described in section “5.3.2 Checking the lock” on
page 29.

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 Maintenance 6

6.5.6 O-ring

Possible problems

• cracks in the sealing lip;

• rubber brittle.
Material required

• O-ring (Part No. 0351302).

Procedure

A. Open the centrifuge housing as described in

section “6.3.1 Opening the lid and removing the
centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Remove the O-ring.
D. Place a new O-ring into the groove and use your
thumb to press it fully in.

6.5.7 Silicone buffer

Possible problems

• defective silicone buffer.

Material required

• ethanol;
• lint-free cloth;
(1)
• Loctite 406;
• silicon buffer (Part No. 0271309). Fig. 6-11: Replacing the silicone buffer
Procedure

A. Switch off the centrifuge.

B. Disconnect the power plug.
C. Remove the silicone buffer to be replaced (1).
D. Clean the adhesive surface with ethanol.
E. Glue a new silicone buffer in place.

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6 Maintenance

6.5.8 Shaft adapter

Possible problems

• shaft is stuck (Alarm: “Low tacho”);

• centrifuge head is stuck on shaft adapter.
Material required

• hexagon key size 2;

• shaft adapter (Part No. 0351208).

Procedure

A. Open the centrifuge housing as described in

section “6.3.1 Opening the lid and removing the (1)
centrifuge head” on page 45.
B. Switch off the centrifuge.
C. Remove the V-ring (3) by manually pulling it (2)
upwards.
D. Unscrew and remove the 3 threaded pins (2).
(3)
E. Pull the shaft adapter (1) from the drive shaft.
F. Insert a new shaft adapter (1) and secure it with
the 3 threaded pins (2).
G. Insert the V-ring (3) by manually pushing it over
the shaft adapter, until the sealing lip touches
slightly the raised part of the tub (4).

(4)

Fig. 6-12: Replacing the shaft adapter

Make sure that the drive shaft with the shaft adapter and the V-ring can move freely.

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 Maintenance 6

6.5.9 V-ring

Possible problems:

• defective V-ring.
Material required

• V-ring (Part No. 0351214).

When replacing the protection ring (with the threaded pins M3x8), which was used for serial
numbers up to 8929.20.001, also the axle must be replaced (too short, Part No. 0351208) or the
current axle must be installed higher.

Procedure

A. Open the centrifuge housing as described in

section “6.3.1 Opening the lid and removing the (1)
centrifuge head” on page 45.
(2)
B. Switch off the centrifuge.
C. Remove the V-ring (1) by manually pulling it
upwards.
D. Grease the rubber sealing lip inside (2) using
long-life grease (for example Motorex 2000 NGLI
2).
E. Insert the V-ring (1) by manually pushing it over
the shaft adapter, until the sealing lip touches (3)
slightly the raised part of the tub (3).
Fig. 6-13: Replacing the V-ring

Make sure that the drive shaft with the shaft adapter and the V-ring can move freely.

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6 Maintenance

6.5.10 Rubber feet

Possible problems

• centrifuge position is no longer stable;

• vibrations.
Material required

• 4 feet (Part No. 0211210).

Procedure

A. Open the centrifuge housing as described in

section “6.3.1 Opening the lid and removing the
centrifuge head” on page 45 unless it is secured
with packing material.
B. Switch off the centrifuge.
C. Disconnect the power plug.
D. Place the centrifuge on one side.
(1)
E. Unscrew the feet (1) by hand.
F. Screw in new feet.

Fig. 6-14: Replacing the feet

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 Maintenance 6

6.5.11 Replacing the magnet in the centrifuge head

Possible problems

• centrifuge head sits loosely on the shaft

adapter.
Material required

• ethanol;
• lint-free cloth;
• sticker for head (Part No. 0351606);
• magnet ø 14 x 4 (Part No. 0351608);
• Loctite 406.
Procedure

A. Remove all components from the tub as (1)

described in section “6.3.1 Opening the lid and
removing the centrifuge head” on page 45. (2)

B. Switch off the centrifuge.

C. Remove the sticker (1) on the centrifuge head.
D. Remove the magnet (2) in the centrifuge head.
E. Clean the glue surface with ethanol.
F. Place a new magnet and glue it in place with
Loctite 406.
G. Glue a new sticker in place.
Fig. 6-15: Replacing the magnet in the centrifuge
head

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6 Maintenance

6.6 Electronical and electrical parts replacement

Spare parts and order numbers are listed in the spare parts catalogue, section “7 Spare parts”
on page 69.

Use original parts only. Otherwise you may cause damage to the centrifuge or put persons at
risk.

6.6.1 Replacing the IES switch assembly

Possible problems

• defective power switch;

• defective fuse holder;
• defective line filter.
Material required

• screwdriver size 0;
• IES switch assembly (Part No. 0241214).
Procedure
(1) (2) (3)
A. Open the centrifuge housing as described in
section “6.3.2 Disassembling the centrifuge
housing” on page 46.
B. Disconnect the connectors (1), (2) and (3) and
mark them.
C. Use the screwdriver to press on the 4 tabs (4)
and pull out the IES switch assembly to the
outside.
D. Remove the mains fuses as described in section (4)
“6.6.2 Replacing the mains fuses” on page 59.
E. Insert a new IES switch assembly from the
outside.

Make sure that all tabs lock in place.

Fig. 6-16: Replacing the IES switch assembly
F. Connect the connectors according to their
markings.
G. Insert the mains fuses.
H. Check the primary voltage as described in
section “5.2 Checking the power supply” on
page 25.
I. Assemble the centrifuge as described in section
“6.4 Assembly” on page 48”.

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 Maintenance 6

6.6.2 Replacing the mains fuses

Possible problems

• defective fuses.
Material required

• 2 fuses T 2 A / 250 V (Part No. 0211705) for 100 – 240 VAC.

Never replace the fuses with fuses with a different power rating.

Procedure

A. Switch off the centrifuge.

B. Disconnect the power plug.
C. Use your index finger to press against the
locking mechanism in the centre of the bottom
side of the fuse holder and remove the fuse
holder.
D. Replace the defective mains fuses.
E. Insert the fuse holder and make sure that it
clicks audibly into place.

Fig. 6-17: Replacing the mains fuses

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6 Maintenance

6.6.3 Power supply board

Possible problems

• centrifuge does not work.

Material required

• torx screwdriver T10;

• power supply board (Part No. 0351212).
Procedure

A. Open the centrifuge housing as described in section “6.3.2 Disassembling the centrifuge housing”
on page 46.
B. Disconnect the connectors (2) and (4).
C. Unscrew and remove the 3 screws (1) and the screw (3) and the grounding cable (5).
D. Remove the power supply board.

(1)

Detail X

(3)
(1)

(5)

(2)

X (1)

(4) (3)

Fig. 6-18: Replacing the power supply board

E. Insert a new power supply board and secure it with the 3 screws (1). Make sure that the
grounding cable connected to connector (2) is routed below the power supply board.
F. Secure the grounding cable (5) with the screw (3).
G. Reconnect all connectors.
H. Check the power supply as described in section “5.2 Checking the power supply” on page 25.

60/90 DiaCent-12 Service Manual • © Bio-Rad

 Maintenance 6

6.6.4 Replacing the controller board

Possible problems

• centrifuge does not work;

• motor does not turn;
• centrifugation time not correct;
• speed not correct;
• alarm: “Low tacho” or “High tacho”;
• display does not work properly;
• lid cannot be opened properly;
• monitoring of lid lock does not work (Alarm: “Check the lid”).
Material required

• torx screwdriver T10

• controller board (Part No. 0351202)

Replacing this board will delete all current parameter and program settings.

A. Open the centrifuge housing as described in section “6.3.2 Disassembling the centrifuge housing”
on page 46.
B. Disconnect the connectors (1), (2), (3), (4), (6) and (7).
C. Unscrew and remove the 4 screws (5).
(1) (2) (3) (4)

(5)
(5)

(5)
(5)

(7) (6)

Fig. 6-19: Replacing the controller board

D. Remove the controller board.

E. Insert a new controller board and secure it with the screws.
F. Reconnect all connectors.
G. Assemble the centrifuge as described in section “6.4 Assembly” on page 48.
H. Perform a functional check as described in section “5.3 Functional check/Calibration” on page 27.

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6 Maintenance

6.6.5 Replacing the membrane keyboard

Possible problems

• aging of the adhesive film;

• contact problems;
(1)
• flat cable torn out.
Material required

• knife;
Fig. 6-20: Replacing the membrane keybord
• ethanol;
• lint-free cloth;
• membrane keyboard (Part No. 0351303).

Procedure

A. Open the centrifuge housing as described in

section “6.3.2 Disassembling the centrifuge
housing” on page 46.
B. Disconnect the connector (1) on the LCD board.
C. Use the knife to remove the membrane keyboard
on the front side.
D. Clean the adhesive surface with ethanol.
E. Glue a new membrane keyboard in place. Fig. 6-21: Replacing the membrane keybord

F. Reconnect the connector.

G. Assemble the centrifuge as described in section
“6.4 Assembly” on page 48.
H. Check the function of the keyboard as described
in section “5.3.1 Checking keyboard and display”
on page 27.

62/90 DiaCent-12 Service Manual • © Bio-Rad

 Maintenance 6

6.6.6 Replacing the LCD board

Possible problems

• contact problems;
• reading incomplete.
Material required

• Allen screwdriver size 1;

• LCD board (Part No. 0351304).
Procedure

A. Open the centrifuge housing as described in

section “6.3.2 Disassembling the centrifuge
housing” on page 46.
B. Disconnect the connectors (1) and (2).
(1)
C. Loosen the 2 screws (3) so far that you can
remove the LCD board. (2)

Fig. 6-22: Replacing the LCD board

Please observe that the board is glued in place.

D. Insert a new LCD board according to the

illustration and secure it with the screws.
E. Reconnect the connectors (1) and (2).
F. Assemble the centrifuge as described in section
“6.4 Assembly” on page 48.
G. Check the function of the LSD display as
described in section “5.3.1 Checking keyboard
and display” on page 27.

(3)

Fig. 6-23: Replacing the LCD board

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6 Maintenance

6.6.7 Replacing the lock

Possible problems

• lid can no longer be opened;

• lid can no longer be closed.
Material required

• torx screwdriver T10;

• lock assembly (Part No. 0241401).
Procedure

A. Open the centrifuge housing as described in section “6.3.2 Disassembling the centrifuge housing”
on page 46.
B. Remove the connectors (8) and (9).

Avoid bending the flat cable sharply.

C. Unscrew and remove the 2 screws (7) and the small mounting plate (6).
D. Loosen the 2 screws (1) and (4) until the Inox washers (2) and (5) start to turn.
E. Remove the lock.

(1) (2) (3) (4) (5) (6) (7)

(8)

(9)

Fig. 6-24: Replacing the lock

F. Insert a new lock into the recess and secure it with the screws, the Inox washers and the
mounting plate.

The lever (3) must move freely to ensure proper locking. Therefore, make sure that the Inox
washer (2) does not block the lever.

G. Close the lid.

H. Lightly compress the spring/lever (10).

The lid should now open again.

64/90 DiaCent-12 Service Manual • © Bio-Rad
 Maintenance 6

(10)

Fig. 6-25: Compress the spring / lever

I. Reconnect the connectors.

J. Assemble the centrifuge as described in section “6.4 Assembly” on page 48.
K. Check the function of the lock as described in section “5.3.2 Checking the lock” on page 29.

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6 Maintenance

6.6.8 24V motor/vibration sensor

Possible problems

• motor :
- motor does not turn;
- motor does not accelerate to nominal
speed.
• vibration sensor :
- incorrect torque of motor mounting
screws;
- detection cannot be adjusted properly.

Material required
(1)
• hexagon key size 3;
• side-cutting pliers;
• hexagon key size 2.0;
(2)
• Loctite 406;
• Loctite 243;
• rubber rings (Part No. 0211120); (4) (3)
• for centrifuges up to serial no. 8920035090:
aluminium rings (Part No. 0351305);
• motor 24 V (Part No. 0351213);
• torque spanner (Part No. 0471002);
• 4 cable ties 100 mm (Part No. 0101418);
• vibration sensor (Part No. 0351308);
• 4 screws (Part No. 0351310).

Procedure

A. Open the centrifuge housing as described in

section “6.3.2 Disassembling the centrifuge
Fig. 6-26: Replacing the 24V motor/vibration sensor
housing” on page 46.
B. Remove the V-ring (3) by manually pulling it
upwards.
C. Loosen the 3 threaded pins (2) on the shaft
adapter (1).
D. Pull the shaft adapter from the shaft.
E. Place the upper part of the housing on the table
with the lid open.
F. While holding the motor, unscrew and remove
the 4 screws (4).

66/90 DiaCent-12 Service Manual • © Bio-Rad

 Maintenance 6

G. Remove the motor unit, the vibration sensor, the

2 rubber rings and the 2 aluminium rings if
existing. (5)

H. Unscrew and remove the screw (5), the toothed (6)

washer (6) and the ground connector (7). (7)
I. Check the 2 rubber rings (11) for damage and
replace them if required.
J. Insert the 4 screws into the holes (9) and (10) in
the tub.
K. For centrifuges without raised parts around the 2
holes (10), place the 2 aluminium rings on the 2
screws (10).
L. Use Loctite 406 to glue the 2 rubber rings onto
the aluminium rings or raised parts (10).
M. To replace the vibration sensor (8), place a new
vibration sensor onto the two screws (9).

Fig. 6-27: Replacing the 24V motor/vibration sensor

N. If you do not replace the vibration sensor (8) with

a new part, clean it with ethanol and place it
onto the two screws (9).
O. Secure the ground connector (7) with the toothed
washer (6) and the screw (5) on the new 24 VDC
motor.
(11)
P. Place 1 drop of Loctite 243 into each thread of (8)
the motor.
Q. Insert a new 24 VDC motor.
R. Align the cable at the motor and at the vibration
sensor.
S. Initially tighten the 4 screws (9) and (10) with 25
cNm. (9)
(10)
T. Subsequently tighten the 2 screws (9) that secure
the vibration sensor with 35 cNm.

Fig. 6-28: Replacing the 24V motor/vibration sensor

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6 Maintenance

U. Secure the cables of the vibration sensor and the motor using the cable ties and on the cable
fixation as shown in the illustration below.

Fig. 6-29: Replacing the 24V motor/vibration sensor

V. Connect all connectors.

(1)
W. Secure the shaft adapter (1) with the 3 threaded
pins (2).
X. Insert the V-ring (3) by manually pushing it over
the shaft adapter, until the sealing lip touches (2)
slightly the raised part of the tub.

Make sure that the drive shaft with the shaft (4) (3)
adapter and the V-ring can move freely.

Y. Double-check the torque of the 4 screws (4).

Z. Assemble the centrifuge as described in section
“6.4 Assembly” on page 48.
AA. Perform a functional check as described in
section “5.3.3 Adjusting the vibration detection
for centrifuges with SN from 892001001” on
page 30, section “5.3.5 Checking nominal speed
and centrifugation time” on page 35, section
“5.3.6 Checking mobility/position of the tube
holders” on page 39 and section “5.3.7 Checking
the motor running time” on page 40. Fig. 6-30: Replacing the 24V motor/vibration sensor

68/90 DiaCent-12 Service Manual • © Bio-Rad

 Spare parts 7

7.1 Spare parts

You can order the following spare parts for centrifuges from your Bio-Rad representative or directly
from the manufacturer.

The spare parts catalogue is structured as follows :

• representation of the lower section, with designation and part number;

• representation of the upper section, with designation and part number;
• additional spare parts, with designation and part number.

7.1.1 Use and application

The spare parts catalogue is used exclusively for identifying and ordering spare parts. For
assembling and dismantling purposes it should be used only in connection with the
corresponding instructions contained in the chapter “5 Checks and adjustments”.

7.1.2 Ordering spare parts

To ensure that the spare parts ordering process runs smoothly and to prevent incorrect deliveries,
please specify the following information :

• centrifuge type;
• serial number;
• spare part designation;
• spare part number;
• quantity.

H005241 V5.0-10/2010 69/90

7 Spare parts

7.2 Lists of spare parts

7.2.1 Lower part of housing

7.2.1.1 Electrical socket assembly

Pos. Description Qty Part No.

01 Ferrite 1 028 12 05
02 Toothed washer M4 - bronze 1 021 12 15
03 Screw flat headed M 4 x 8 1 035 12 11
04 Electrical socket 1 024 12 14
05 Electrical cables 240 V 1 035 12 07
06 Fuses T2A for 100 – 240 VAC 1 021 17 05

7.2.1.2 Power supply assembly

Pos. Description Qty Part No.

07 Power supply 60W 1 035 12 12

08 Electrical wiring 24 V 1 035 12 03
09 Screw PT M3 x 10 - convex 1 027 11 14

7.2.1.3 Circuit board assembly

Pos. Description Qty Part No.

10 Screw PT M3 x 10 - convex 1 027 11 14

11 Circuit board 1 035 12 02
12 Flat cable 1 024 12 12

7.2.1.4 Lower frame assembly

Pos. Description Qty Part No.

13 Feet 1 021 12 10
14 Screw M 4 x 12 1 024 13 12
15 Lower frame 1 035 12 01
16 Self-adhesive cable fixation 1 036 12 04

70/90 DiaCent-12 Service Manual • © Bio-Rad

 Spare parts 7

01 02 03 04 05

06

07
16

15

08

14
09

13

12 11 10

Fig. 7-1: Spare parts, lower section

H005241 V5.0-10/2010 71/90

7 Spare parts

7.2.2 Upper section

7.2.2.1 Lid assembly

Pos. Description Qty Part No.

01 Hook assembly 1 024 15 04

02 Screw cover 1 024 15 03
03 Screw M 4 x 14 - cylindrical 1 027 13 11
04 Plexiglas lid 1 035 15 01
05 Screw cover 1 024 15 03
06 Screw M 4 x 20 - cylindrical 1 025 15 07
07 Hinge 1 024 13 03
08 Cushion 1 024 13 10
09 Silicone buffer 1 027 13 09
10 Pin inox ø5 x 60 1 024 13 09
11 Axle holder 1 024 13 08
12 Screw PT M3 x 10 - convex 1 027 11 14
36 Spring for hinge 1 024 13 14

7.2.2.2 Lid lock assembly

Pos. Description Qty Part No.

20 Screw PT M3 x 10 - convex 1 027 11 14

21 Latch holder 1 024 13 05
22 Screw PT M3 x 10 - convex 1 027 11 14
24 Lid lock + 60 mm cable 1 024 14 01
25 Lock connector 1 024 14 02
26 Electrical wiring 24V 1 035 12 03

7.2.2.3 Display assembly

Pos. Description Qty Part No.

23 Washer inox ø 20 x 2 1 024 13 04

27 Screw PT M 2.2 x 6 1 024 13 11
28 Display holder 1 024 13 07
29 Display 1 035 13 04
31 Membrane keyboard 1 035 13 03

72/90 DiaCent-12 Service Manual • © Bio-Rad

 Spare parts 7

01 02

39
38
03
37

36 04

05
35
34 06

33
07
08
09
32
10
11
12

31

13

40

14
30

15
29
16
28
27 17

26 25 24 23 22 21 20 19 18

Fig. 7-2: Spare parts, upper section

H005241 V5.0-10/2010 73/90

7 Spare parts

7.2.2.4 Motor assembly

Pos. Description Qty Part No.

15 Motor brushless 24 VDC 1 035 12 13

16 Rubber washer M4 x 12 1 021 11 20
17 Aluminium ring, only for centrifuges up to serial 1 035 13 05
no. 8920035090
18 Vibration detector 1 035 13 08

7.2.2.5 Centrifuge head and motor axle assembly

Pos. Description Qty Part No.

32 Screws M 5 x 16 - convex 1 035 13 10

33 Sealing V-ring 1 035 12 14
34 Screws M 4 x 6 - w/o head 1 036 13 08
35 Motor axle 1 035 12 08
37 DiaCent-12 head 1 840010
38 Magnet ø 14 x 4 1 035 16 08
39 Sticker 1 035 16 06

7.2.2.6 Upper frame assembly

Pos. Description Qty Part No.

13 Rubber gasket 1 035 13 02

14 Upper frame 1 035 13 01
19 Self-adhesive cable fixation 1 027 12 06
30 Fixation plate, 20x20 mm 1 010 14 17
40 Sticker Biohazard 1 E 900000

74/90 DiaCent-12 Service Manual • © Bio-Rad

 Spare parts 7

01 02

39
38
03
37

36 04

05
35
34 06

33
07
08
09
32
10
11
12

31

13

40

14
30

15
29
16
28
27 17

26 25 24 23 22 21 20 19 18

Fig. 7-3: Spare parts, upper section

H005241 V5.0-10/2010 75/90

7 Spare parts

7.2.2.7 Additional spare parts

Pos. Description Qty Part No.

--- Foam, 1x 2 pces 1 035 17 01

--- Foam, head 1 035 17 02
--- Box DC12 / ID 12 S II 1 VP00607

76/90 DiaCent-12 Service Manual • © Bio-Rad

 Decommissioning 8

8.1 Disposal of waste equipment

This symbol on the identification plate indicates
that this product may not be disposed of with
other waste. For disposal of this product, hand
this instrument to a designated collection point
for the recycling of electrical and electronic
equipment waste.

Contact your local waste disposal service for

more information.

Fig. 8-1: WEEE symbol

H005241 V5.0-10/2010 77/90

8 Decommissioning

78/90 DiaCent-12 Service Manual • © Bio-Rad

 Appendix 9

9.1 Equipment

9.1.1 Standard accessories

Device Qty Part No.

DiaCent-12 for 100–240 VAC 1 840000

Power cord EURO 1 010 15 00
Power cord CH 1 030 13 00
Power cord US 1 010 23 00
Power cord UK 1 031 43 00
User manual, English 1 H009121
Identification sticker 1 E750830

9.1.2 Special accessories

Device Qty Part No.

Stroboscope 1 047 10 03

9.2 Technical Documentation

9.2.1 User Manual

English ................................................................H 009121

German ...............................................................H 009077
Italian ..................................................................H 009078
Spanish...............................................................H 009079
Portuguese .........................................................H 009080
Greek ..................................................................H 009081
Swedish ..............................................................H 009082
Danish.................................................................H 009083
French.................................................................H 009084
Norwegian ..........................................................H 009114
Croatian ..............................................................H 009110

9.2.2 Service Manual (english only)

DiaCent-12 .........................................................H 005241

H005241 V5.0-10/2010 79/90

9 Appendix

9.3  Conformity
The DiaCent-12, which is "IVD" marked on the identification plate, has been designed, constructed
and fabricated in compliance with the following guidelines and standards :

• 98/79/EC;
• 73/23/EEC;
• EN 61326:1997 + A1:1998 + A2:2001;
• EN 61000-3-2:2000;
• EN 61000-3-3:1995 + A1:2001;
• EN 61010-1:2001;
• EN 61010-2-101:2002;
• EN 61010-2-020:1992 + A1:1996.

The EU declaration of conformity which applies is supplied with the centrifuge.

9.4 Documents / Forms

Except for the maintenance table, the official electronic documents can be found on the Bio-
Rad website: under Distributors - Secret Area - Official forms.

80/90 DiaCent-12 Service Manual • © Bio-Rad

 Appendix 9

9.4.1 Reception form

DiaMed GmbH
Help Desk Instruments
1785 Cressier FR - Switzerland
Tel.: +41 26 674 51 60
Fax: +41 26 674 51 18
Email: [email protected]

Reception Form

Applicable for IHD Instruments

Distributor/Subsidiary: Instrument type:

Country: Instrument #:

Date of check:

Yes No Remarks:

Tool check (quantity & quality according to the

packing List)

Visual Check

Functional check (Global initialisation)

Shockwatch intact (relevant instruments)

General remarks:

IMPORTANT!
This form must be returned to the address above within 6 weeks after invoice date.

Date Service engineer's name (print name)

Service engineer's signature

03.24.101 V03.01

H005241 V5.0-10/2010 81/90

9 Appendix

9.4.2 Installation protocol

DiaMed GmbH
Help Desk Instruments
1785 Cressier FR - Switzerland
Tel.: +41 26 674 51 60
Fax: +41 26 674 51 18
Email: [email protected]

Installation protocol
Applicable for IHD Centrifuges and Incubators

Distributor/Subsidiary: Instrument type:

Country: Instrument #:

Installation date:

General Yes No Remarks

Visual check of the Instrument

Main functions

Display

Training

First run test

General remarks:

IMPORTANT
With this document the final customer certifies that this instrument is fully functional, and that the necessary
information was received in order to use the instrument correctly. After being signed by both parties, the
form must be returned to the address above.

Date Service engineer's name (print name)

Customer's stamp & signature Service engineer's signature

03.24.109 V03.01

82/90 DiaCent-12 Service Manual • © Bio-Rad

 Appendix 9

9.4.3 Decontamination certificate

The DiaCent-12 and its accessories must be cleaned and decontaminated before performing any
maintenance, revision task, before taking the instrument out of service and when preparing for
transportation.

You can find the official decontamination certificate on the Bio-Rad website, section
«distributors -secret area - official forms».

Fill in a copy of the decontamination certificate and place it on the DiaCent-12, in a conspicuous
place.

The absence of decontamination certificate may extend the time needed for revision and
maintenance.

Bio-Rad will bill any cleaning and decontamination operation.

The original certificate must remain in the present manual.

When a certificate is required, make a copy and fill in the latter sample.

H005241 V5.0-10/2010 83/90

9 Appendix

DiaMed GmbH
Help Desk Instruments
1785 Cressier FR - Switzerland
Tel.: +41 26 674 51 60
Fax: +41 26 674 51 18
Email: [email protected]

Decontamination Certificate
IHD Instruments

Decontamination
performed by:

Country:

E-mail:

Phone:

I, hereby declare, that the instrument below:

Instrument:

Material No.:

Instrument No.:

has been cleaned and decontaminated according to the

procedure described in the relevant instrument manual, in the
paragraph “maintenance ”.

Cleaning & decontamination performed by:

Date: Signature:

Return to:
DiaMed GmbH, Help Desk Instruments, E-Mail: [email protected] or fax +41 26 674 51 18

03.24.125 V02.01

84/90 DiaCent-12 Service Manual • © Bio-Rad

 Appendix 9

9.5 Maintenance record

The original blank maintenance sheet must remain in the present manual.
When a maintenance record is required, make a copy and fill in the latter sample.

Type Page

Serial number

Maintenance / Defect Action Operator Date

H005241 V5.0-10/2010 85/90

9 Appendix

Maintenance / Defect Action Operator Date

86/90 DiaCent-12 Service Manual • © Bio-Rad

 Appendix 9

9.6 Identification plate

The identification plate on the back of the (1) (2) (3) (4)
centrifuge is used for identification and contains
the following information:

(1) Serial number

(2) Nominal speed
(3) Type
(4) Article number
(5) Mains connection
(voltage, frequency, power consumption)
(6) Mains fuse for 100–240 VAC version
(7) IVD In vitro diagnostic medical device (5) (6) (7) (8)

(8) Date of manufacture (year + month)

Fig. 9-1: Identification plate
Please copy the following information from the
identification plate in the fields below:

Type

Serial number

H005241 V5.0-10/2010 87/90

 9.7

88/90
9 Appendix

F1 Power supply board

L (24 VDC)
Block diagram

TB1
F2 Part No. 035 12 12
N

Fig. 9-2: Block diagram

Electrical wiring 100 – 240 V TB2
Part No. 035 12 07
Power supply

Part No. 024 12 14

Electrical wiring 24 V
Vibration detector Part No. 035 12 03
Part No. 035 13 08

X11
X9
X8
Controller board
Motor Part No. 035 12 02
(24 VDC) X6
X7

x14
X13
Part No. 035 12 13
Lock assembly
Flat cable Part No. 024 14 01
Part No. 024 12 12

LCD board
Part No. 035 13 04

Membrane keyboard
Part No. 035 13 03

DiaCent-12 Service Manual • © Bio-Rad

 Notes

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H005241 V5.0-10/2010 89/90

 Bio-Rad Bio-Rad Laboratories is a multinational manufacturer and distributor
Laboratories of clinical diagnostics and life science research products.

Clinical DiaMed
1785 Cressier FR
Diagnostics Group Switzerland
www.bio-rad.com/immunohematology

© Bio-Rad H005241 V5.0-10/2010